PMC:1310901 / 19754-20782 JSONTXT

{"project":"bionlp-st-ge-2016-reference-eval","denotations":[{"id":"T10135","span":{"begin":479,"end":488},"obj":"Regulation"},{"id":"T10136","span":{"begin":516,"end":524},"obj":"Gene_expression"},{"id":"T10137","span":{"begin":626,"end":634},"obj":"Gene_expression"},{"id":"T10138","span":{"begin":684,"end":694},"obj":"Regulation"},{"id":"T10139","span":{"begin":725,"end":733},"obj":"Gene_expression"},{"id":"T10140","span":{"begin":27,"end":32},"obj":"Protein"},{"id":"T10141","span":{"begin":489,"end":497},"obj":"Entity"},{"id":"T10142","span":{"begin":698,"end":706},"obj":"Entity"},{"id":"T10143","span":{"begin":781,"end":792},"obj":"Regulation"},{"id":"T10144","span":{"begin":151,"end":156},"obj":"Protein"},{"id":"T10145","span":{"begin":214,"end":219},"obj":"Protein"},{"id":"T10146","span":{"begin":450,"end":455},"obj":"Protein"},{"id":"T10147","span":{"begin":510,"end":515},"obj":"Protein"},{"id":"T10148","span":{"begin":620,"end":625},"obj":"Protein"},{"id":"T10149","span":{"begin":719,"end":724},"obj":"Protein"},{"id":"T10150","span":{"begin":844,"end":848},"obj":"Protein"},{"id":"T10151","span":{"begin":1013,"end":1018},"obj":"Protein"}],"relations":[{"id":"R8404","pred":"themeOf","subj":"T10141","obj":"T10135"},{"id":"R8405","pred":"partOf","subj":"T10141","obj":"T10146"},{"id":"R8406","pred":"themeOf","subj":"T10142","obj":"T10138"},{"id":"R8407","pred":"partOf","subj":"T10142","obj":"T10149"},{"id":"R8408","pred":"themeOf","subj":"T10147","obj":"T10136"},{"id":"R8409","pred":"themeOf","subj":"T10148","obj":"T10137"},{"id":"R8410","pred":"themeOf","subj":"T10149","obj":"T10139"},{"id":"R8411","pred":"themeOf","subj":"T10150","obj":"T10143"}],"text":"In vitro methylation of an IRF-4 promoter-reporter construct decreases its activity\nTo provide evidence for a direct effect of methylational status on IRF-4 promoter activity we performed reporter gene assays with IRF-4 promoter constructs before and after their in vitro methylation. A complete methylation of these constructs was checked via restriction assays with methylation-sensitive endonucleases (Figure 5A). Intriguingly, methylation of the IRF-4 promoter significantly decreased promoter activity in IRF-4-positive SD-1 cells by 85.0% (Figure 5B). The silencing effect of CpG methylation was not restricted to IRF-4-positive cells, since in vitro methylation led to a 92.9% abrogation of promoter activity in IRF-4-negative Jurkat cells (Figure 5C). In contrast, control methylation of a reporter construct with a different promoter (FasL) as well as an empty vector had no effect on the reporter activity (data not shown). These data proved a direct association between methylation and activity of the IRF-4 promoter."}

{"project":"events-check-again","denotations":[{"id":"T10105","span":{"begin":27,"end":32},"obj":"Protein"},{"id":"T10106","span":{"begin":151,"end":156},"obj":"Protein"},{"id":"T10107","span":{"begin":214,"end":219},"obj":"Protein"},{"id":"T10108","span":{"begin":450,"end":455},"obj":"Protein"},{"id":"T10109","span":{"begin":479,"end":488},"obj":"Negative_regulation"},{"id":"T10110","span":{"begin":489,"end":497},"obj":"Entity"},{"id":"T10111","span":{"begin":510,"end":515},"obj":"Protein"},{"id":"T10112","span":{"begin":516,"end":524},"obj":"Gene_expression"},{"id":"T10113","span":{"begin":620,"end":625},"obj":"Protein"},{"id":"T10114","span":{"begin":626,"end":634},"obj":"Gene_expression"},{"id":"T10115","span":{"begin":684,"end":694},"obj":"Negative_regulation"},{"id":"T10116","span":{"begin":698,"end":706},"obj":"Entity"},{"id":"T10117","span":{"begin":719,"end":724},"obj":"Protein"},{"id":"T10118","span":{"begin":725,"end":733},"obj":"Gene_expression"},{"id":"T10119","span":{"begin":844,"end":848},"obj":"Protein"},{"id":"T10120","span":{"begin":1013,"end":1018},"obj":"Protein"}],"relations":[{"id":"R8393","pred":"themeOf","subj":"T10110","obj":"T10109"},{"id":"R8394","pred":"partOf","subj":"T10110","obj":"T10108"},{"id":"R8395","pred":"themeOf","subj":"T10111","obj":"T10112"},{"id":"R8396","pred":"themeOf","subj":"T10113","obj":"T10114"},{"id":"R8397","pred":"themeOf","subj":"T10116","obj":"T10115"},{"id":"R8398","pred":"partOf","subj":"T10116","obj":"T10117"},{"id":"R8399","pred":"themeOf","subj":"T10117","obj":"T10118"}],"attributes":[{"id":"M217","pred":"Negation","subj":"T10118","obj":"true"}],"text":"In vitro methylation of an IRF-4 promoter-reporter construct decreases its activity\nTo provide evidence for a direct effect of methylational status on IRF-4 promoter activity we performed reporter gene assays with IRF-4 promoter constructs before and after their in vitro methylation. A complete methylation of these constructs was checked via restriction assays with methylation-sensitive endonucleases (Figure 5A). Intriguingly, methylation of the IRF-4 promoter significantly decreased promoter activity in IRF-4-positive SD-1 cells by 85.0% (Figure 5B). The silencing effect of CpG methylation was not restricted to IRF-4-positive cells, since in vitro methylation led to a 92.9% abrogation of promoter activity in IRF-4-negative Jurkat cells (Figure 5C). In contrast, control methylation of a reporter construct with a different promoter (FasL) as well as an empty vector had no effect on the reporter activity (data not shown). These data proved a direct association between methylation and activity of the IRF-4 promoter."}

{"project":"GO-CC","denotations":[{"id":"T10054","span":{"begin":530,"end":535},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T10055","span":{"begin":635,"end":640},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T10056","span":{"begin":741,"end":746},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"In vitro methylation of an IRF-4 promoter-reporter construct decreases its activity\nTo provide evidence for a direct effect of methylational status on IRF-4 promoter activity we performed reporter gene assays with IRF-4 promoter constructs before and after their in vitro methylation. A complete methylation of these constructs was checked via restriction assays with methylation-sensitive endonucleases (Figure 5A). Intriguingly, methylation of the IRF-4 promoter significantly decreased promoter activity in IRF-4-positive SD-1 cells by 85.0% (Figure 5B). The silencing effect of CpG methylation was not restricted to IRF-4-positive cells, since in vitro methylation led to a 92.9% abrogation of promoter activity in IRF-4-negative Jurkat cells (Figure 5C). In contrast, control methylation of a reporter construct with a different promoter (FasL) as well as an empty vector had no effect on the reporter activity (data not shown). These data proved a direct association between methylation and activity of the IRF-4 promoter."}

{"project":"sentences","denotations":[{"id":"T9690","span":{"begin":0,"end":83},"obj":"Sentence"},{"id":"T9691","span":{"begin":84,"end":284},"obj":"Sentence"},{"id":"T9692","span":{"begin":285,"end":416},"obj":"Sentence"},{"id":"T9693","span":{"begin":417,"end":557},"obj":"Sentence"},{"id":"T9694","span":{"begin":558,"end":759},"obj":"Sentence"},{"id":"T9695","span":{"begin":760,"end":933},"obj":"Sentence"},{"id":"T9696","span":{"begin":934,"end":1028},"obj":"Sentence"},{"id":"T141","span":{"begin":0,"end":83},"obj":"Sentence"},{"id":"T142","span":{"begin":84,"end":284},"obj":"Sentence"},{"id":"T143","span":{"begin":285,"end":416},"obj":"Sentence"},{"id":"T144","span":{"begin":417,"end":557},"obj":"Sentence"},{"id":"T145","span":{"begin":558,"end":759},"obj":"Sentence"},{"id":"T146","span":{"begin":760,"end":933},"obj":"Sentence"},{"id":"T147","span":{"begin":934,"end":1028},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"In vitro methylation of an IRF-4 promoter-reporter construct decreases its activity\nTo provide evidence for a direct effect of methylational status on IRF-4 promoter activity we performed reporter gene assays with IRF-4 promoter constructs before and after their in vitro methylation. A complete methylation of these constructs was checked via restriction assays with methylation-sensitive endonucleases (Figure 5A). Intriguingly, methylation of the IRF-4 promoter significantly decreased promoter activity in IRF-4-positive SD-1 cells by 85.0% (Figure 5B). The silencing effect of CpG methylation was not restricted to IRF-4-positive cells, since in vitro methylation led to a 92.9% abrogation of promoter activity in IRF-4-negative Jurkat cells (Figure 5C). In contrast, control methylation of a reporter construct with a different promoter (FasL) as well as an empty vector had no effect on the reporter activity (data not shown). These data proved a direct association between methylation and activity of the IRF-4 promoter."}

{"project":"pmc-enju-pas","denotations":[{"id":"T9706","span":{"begin":0,"end":2},"obj":"FW"},{"id":"T9707","span":{"begin":3,"end":8},"obj":"FW"},{"id":"T9708","span":{"begin":9,"end":20},"obj":"NN"},{"id":"T9709","span":{"begin":21,"end":23},"obj":"IN"},{"id":"T9710","span":{"begin":24,"end":26},"obj":"DT"},{"id":"T9711","span":{"begin":27,"end":32},"obj":"NN"},{"id":"T9712","span":{"begin":33,"end":50},"obj":"JJ"},{"id":"T9713","span":{"begin":51,"end":60},"obj":"NN"},{"id":"T9714","span":{"begin":61,"end":70},"obj":"VB"},{"id":"T9715","span":{"begin":71,"end":74},"obj":"PRP-DOLLAR-"},{"id":"T9716","span":{"begin":75,"end":83},"obj":"NN"},{"id":"T9717","span":{"begin":84,"end":86},"obj":"TO"},{"id":"T9718","span":{"begin":87,"end":94},"obj":"VB"},{"id":"T9719","span":{"begin":95,"end":103},"obj":"NN"},{"id":"T9720","span":{"begin":104,"end":107},"obj":"IN"},{"id":"T9721","span":{"begin":108,"end":109},"obj":"DT"},{"id":"T9722","span":{"begin":110,"end":116},"obj":"JJ"},{"id":"T9723","span":{"begin":117,"end":123},"obj":"NN"},{"id":"T9724","span":{"begin":124,"end":126},"obj":"IN"},{"id":"T9725","span":{"begin":127,"end":140},"obj":"JJ"},{"id":"T9726","span":{"begin":141,"end":147},"obj":"NN"},{"id":"T9727","span":{"begin":148,"end":150},"obj":"IN"},{"id":"T9728","span":{"begin":151,"end":156},"obj":"NN"},{"id":"T9729","span":{"begin":157,"end":165},"obj":"NN"},{"id":"T9730","span":{"begin":166,"end":174},"obj":"NN"},{"id":"T9731","span":{"begin":175,"end":177},"obj":"PRP"},{"id":"T9732","span":{"begin":178,"end":187},"obj":"VB"},{"id":"T9733","span":{"begin":188,"end":196},"obj":"NN"},{"id":"T9734","span":{"begin":197,"end":201},"obj":"NN"},{"id":"T9735","span":{"begin":202,"end":208},"obj":"NN"},{"id":"T9736","span":{"begin":209,"end":213},"obj":"IN"},{"id":"T9737","span":{"begin":214,"end":219},"obj":"NN"},{"id":"T9738","span":{"begin":220,"end":228},"obj":"NN"},{"id":"T9739","span":{"begin":229,"end":239},"obj":"NN"},{"id":"T9740","span":{"begin":240,"end":246},"obj":"IN"},{"id":"T9741","span":{"begin":247,"end":250},"obj":"CC"},{"id":"T9742","span":{"begin":251,"end":256},"obj":"IN"},{"id":"T9743","span":{"begin":257,"end":262},"obj":"PRP-DOLLAR-"},{"id":"T9744","span":{"begin":263,"end":265},"obj":"FW"},{"id":"T9745","span":{"begin":266,"end":271},"obj":"FW"},{"id":"T9746","span":{"begin":272,"end":283},"obj":"NN"},{"id":"T9747","span":{"begin":285,"end":286},"obj":"DT"},{"id":"T9748","span":{"begin":287,"end":295},"obj":"JJ"},{"id":"T9749","span":{"begin":296,"end":307},"obj":"NN"},{"id":"T9750","span":{"begin":308,"end":310},"obj":"IN"},{"id":"T9751","span":{"begin":311,"end":316},"obj":"DT"},{"id":"T9752","span":{"begin":317,"end":327},"obj":"NN"},{"id":"T9753","span":{"begin":328,"end":331},"obj":"VB"},{"id":"T9754","span":{"begin":332,"end":339},"obj":"VB"},{"id":"T9755","span":{"begin":340,"end":343},"obj":"IN"},{"id":"T9756","span":{"begin":344,"end":355},"obj":"NN"},{"id":"T9757","span":{"begin":356,"end":362},"obj":"NN"},{"id":"T9758","span":{"begin":363,"end":367},"obj":"IN"},{"id":"T9759","span":{"begin":368,"end":389},"obj":"JJ"},{"id":"T9760","span":{"begin":390,"end":403},"obj":"NN"},{"id":"T9761","span":{"begin":404,"end":405},"obj":"-LRB-"},{"id":"T9762","span":{"begin":405,"end":411},"obj":"NN"},{"id":"T9763","span":{"begin":412,"end":414},"obj":"NN"},{"id":"T9764","span":{"begin":414,"end":415},"obj":"-RRB-"},{"id":"T9765","span":{"begin":417,"end":429},"obj":"RB"},{"id":"T9766","span":{"begin":429,"end":430},"obj":"-COMMA-"},{"id":"T9767","span":{"begin":431,"end":442},"obj":"NN"},{"id":"T9768","span":{"begin":443,"end":445},"obj":"IN"},{"id":"T9769","span":{"begin":446,"end":449},"obj":"DT"},{"id":"T9770","span":{"begin":450,"end":455},"obj":"NN"},{"id":"T9771","span":{"begin":456,"end":464},"obj":"NN"},{"id":"T9772","span":{"begin":465,"end":478},"obj":"RB"},{"id":"T9773","span":{"begin":479,"end":488},"obj":"VB"},{"id":"T9774","span":{"begin":489,"end":497},"obj":"NN"},{"id":"T9775","span":{"begin":498,"end":506},"obj":"NN"},{"id":"T9776","span":{"begin":507,"end":509},"obj":"IN"},{"id":"T9777","span":{"begin":510,"end":524},"obj":"JJ"},{"id":"T9778","span":{"begin":525,"end":529},"obj":"NN"},{"id":"T9779","span":{"begin":530,"end":535},"obj":"NN"},{"id":"T9780","span":{"begin":536,"end":538},"obj":"IN"},{"id":"T9781","span":{"begin":539,"end":543},"obj":"CD"},{"id":"T9782","span":{"begin":543,"end":544},"obj":"NN"},{"id":"T9783","span":{"begin":545,"end":546},"obj":"-LRB-"},{"id":"T9784","span":{"begin":546,"end":552},"obj":"NN"},{"id":"T9785","span":{"begin":553,"end":555},"obj":"NN"},{"id":"T9786","span":{"begin":555,"end":556},"obj":"-RRB-"},{"id":"T9787","span":{"begin":558,"end":561},"obj":"DT"},{"id":"T9788","span":{"begin":562,"end":571},"obj":"JJ"},{"id":"T9789","span":{"begin":572,"end":578},"obj":"NN"},{"id":"T9790","span":{"begin":579,"end":581},"obj":"IN"},{"id":"T9791","span":{"begin":582,"end":585},"obj":"NN"},{"id":"T9792","span":{"begin":586,"end":597},"obj":"NN"},{"id":"T9793","span":{"begin":598,"end":601},"obj":"VB"},{"id":"T9794","span":{"begin":602,"end":605},"obj":"RB"},{"id":"T9795","span":{"begin":606,"end":616},"obj":"JJ"},{"id":"T9796","span":{"begin":617,"end":619},"obj":"TO"},{"id":"T9797","span":{"begin":620,"end":634},"obj":"JJ"},{"id":"T9798","span":{"begin":635,"end":640},"obj":"NN"},{"id":"T9799","span":{"begin":640,"end":641},"obj":"-COMMA-"},{"id":"T9800","span":{"begin":642,"end":647},"obj":"IN"},{"id":"T9801","span":{"begin":648,"end":650},"obj":"FW"},{"id":"T9802","span":{"begin":651,"end":656},"obj":"FW"},{"id":"T9803","span":{"begin":657,"end":668},"obj":"NN"},{"id":"T9804","span":{"begin":669,"end":672},"obj":"VB"},{"id":"T9805","span":{"begin":673,"end":675},"obj":"TO"},{"id":"T9806","span":{"begin":676,"end":677},"obj":"DT"},{"id":"T9807","span":{"begin":678,"end":682},"obj":"CD"},{"id":"T9808","span":{"begin":682,"end":683},"obj":"NN"},{"id":"T9809","span":{"begin":684,"end":694},"obj":"NN"},{"id":"T9810","span":{"begin":695,"end":697},"obj":"IN"},{"id":"T9811","span":{"begin":698,"end":706},"obj":"NN"},{"id":"T9812","span":{"begin":707,"end":715},"obj":"NN"},{"id":"T9813","span":{"begin":716,"end":718},"obj":"IN"},{"id":"T9814","span":{"begin":719,"end":733},"obj":"JJ"},{"id":"T9815","span":{"begin":734,"end":740},"obj":"NN"},{"id":"T9816","span":{"begin":741,"end":746},"obj":"NN"},{"id":"T9817","span":{"begin":747,"end":748},"obj":"-LRB-"},{"id":"T9818","span":{"begin":748,"end":754},"obj":"NN"},{"id":"T9819","span":{"begin":755,"end":757},"obj":"NN"},{"id":"T9820","span":{"begin":757,"end":758},"obj":"-RRB-"},{"id":"T9821","span":{"begin":760,"end":762},"obj":"IN"},{"id":"T9822","span":{"begin":763,"end":771},"obj":"NN"},{"id":"T9823","span":{"begin":771,"end":772},"obj":"-COMMA-"},{"id":"T9824","span":{"begin":773,"end":780},"obj":"NN"},{"id":"T9825","span":{"begin":781,"end":792},"obj":"NN"},{"id":"T9826","span":{"begin":793,"end":795},"obj":"IN"},{"id":"T9827","span":{"begin":796,"end":797},"obj":"DT"},{"id":"T9828","span":{"begin":798,"end":806},"obj":"NN"},{"id":"T9829","span":{"begin":807,"end":816},"obj":"NN"},{"id":"T9830","span":{"begin":817,"end":821},"obj":"IN"},{"id":"T9831","span":{"begin":822,"end":823},"obj":"DT"},{"id":"T9832","span":{"begin":824,"end":833},"obj":"JJ"},{"id":"T9833","span":{"begin":834,"end":842},"obj":"NN"},{"id":"T9834","span":{"begin":843,"end":844},"obj":"-LRB-"},{"id":"T9835","span":{"begin":844,"end":848},"obj":"NN"},{"id":"T9836","span":{"begin":848,"end":849},"obj":"-RRB-"},{"id":"T9837","span":{"begin":850,"end":852},"obj":"RB"},{"id":"T9838","span":{"begin":853,"end":857},"obj":"RB"},{"id":"T9839","span":{"begin":858,"end":860},"obj":"IN"},{"id":"T9840","span":{"begin":861,"end":863},"obj":"DT"},{"id":"T9841","span":{"begin":864,"end":869},"obj":"JJ"},{"id":"T9842","span":{"begin":870,"end":876},"obj":"NN"},{"id":"T9843","span":{"begin":877,"end":880},"obj":"VB"},{"id":"T9844","span":{"begin":881,"end":883},"obj":"DT"},{"id":"T9845","span":{"begin":884,"end":890},"obj":"NN"},{"id":"T9846","span":{"begin":891,"end":893},"obj":"IN"},{"id":"T9847","span":{"begin":894,"end":897},"obj":"DT"},{"id":"T9848","span":{"begin":898,"end":906},"obj":"NN"},{"id":"T9849","span":{"begin":907,"end":915},"obj":"NN"},{"id":"T9850","span":{"begin":916,"end":917},"obj":"-LRB-"},{"id":"T9851","span":{"begin":917,"end":921},"obj":"NN"},{"id":"T9852","span":{"begin":922,"end":925},"obj":"RB"},{"id":"T9853","span":{"begin":926,"end":931},"obj":"VB"},{"id":"T9854","span":{"begin":931,"end":932},"obj":"-RRB-"},{"id":"T9855","span":{"begin":934,"end":939},"obj":"DT"},{"id":"T9856","span":{"begin":940,"end":944},"obj":"NN"},{"id":"T9857","span":{"begin":945,"end":951},"obj":"VB"},{"id":"T9858","span":{"begin":952,"end":953},"obj":"DT"},{"id":"T9859","span":{"begin":954,"end":960},"obj":"JJ"},{"id":"T9860","span":{"begin":961,"end":972},"obj":"NN"},{"id":"T9861","span":{"begin":973,"end":980},"obj":"IN"},{"id":"T9862","span":{"begin":981,"end":992},"obj":"NN"},{"id":"T9863","span":{"begin":993,"end":996},"obj":"CC"},{"id":"T9864","span":{"begin":997,"end":1005},"obj":"NN"},{"id":"T9865","span":{"begin":1006,"end":1008},"obj":"IN"},{"id":"T9866","span":{"begin":1009,"end":1012},"obj":"DT"},{"id":"T9867","span":{"begin":1013,"end":1018},"obj":"NN"},{"id":"T9868","span":{"begin":1019,"end":1027},"obj":"NN"}],"relations":[{"id":"R8042","pred":"arg1Of","subj":"T9707","obj":"T9706"},{"id":"R8043","pred":"arg1Of","subj":"T9708","obj":"T9707"},{"id":"R8044","pred":"arg1Of","subj":"T9708","obj":"T9709"},{"id":"R8045","pred":"arg1Of","subj":"T9708","obj":"T9714"},{"id":"R8046","pred":"arg2Of","subj":"T9713","obj":"T9709"},{"id":"R8047","pred":"arg1Of","subj":"T9713","obj":"T9710"},{"id":"R8048","pred":"arg1Of","subj":"T9713","obj":"T9711"},{"id":"R8049","pred":"arg1Of","subj":"T9713","obj":"T9712"},{"id":"R8050","pred":"arg2Of","subj":"T9716","obj":"T9714"},{"id":"R8051","pred":"arg1Of","subj":"T9716","obj":"T9715"},{"id":"R8052","pred":"arg1Of","subj":"T9718","obj":"T9717"},{"id":"R8053","pred":"arg2Of","subj":"T9719","obj":"T9718"},{"id":"R8054","pred":"arg1Of","subj":"T9719","obj":"T9720"},{"id":"R8055","pred":"arg2Of","subj":"T9723","obj":"T9720"},{"id":"R8056","pred":"arg1Of","subj":"T9723","obj":"T9721"},{"id":"R8057","pred":"arg1Of","subj":"T9723","obj":"T9722"},{"id":"R8058","pred":"arg1Of","subj":"T9723","obj":"T9724"},{"id":"R8059","pred":"arg1Of","subj":"T9723","obj":"T9727"},{"id":"R8060","pred":"arg2Of","subj":"T9726","obj":"T9724"},{"id":"R8061","pred":"arg1Of","subj":"T9726","obj":"T9725"},{"id":"R8062","pred":"arg2Of","subj":"T9730","obj":"T9727"},{"id":"R8063","pred":"arg1Of","subj":"T9730","obj":"T9728"},{"id":"R8064","pred":"arg1Of","subj":"T9730","obj":"T9729"},{"id":"R8065","pred":"arg1Of","subj":"T9731","obj":"T9718"},{"id":"R8066","pred":"arg1Of","subj":"T9731","obj":"T9732"},{"id":"R8067","pred":"modOf","subj":"T9732","obj":"T9717"},{"id":"R8068","pred":"arg1Of","subj":"T9732","obj":"T9740"},{"id":"R8069","pred":"arg1Of","subj":"T9732","obj":"T9742"},{"id":"R8070","pred":"arg2Of","subj":"T9735","obj":"T9732"},{"id":"R8071","pred":"arg1Of","subj":"T9735","obj":"T9733"},{"id":"R8072","pred":"arg1Of","subj":"T9735","obj":"T9734"},{"id":"R8073","pred":"arg1Of","subj":"T9735","obj":"T9736"},{"id":"R8074","pred":"arg2Of","subj":"T9739","obj":"T9736"},{"id":"R8075","pred":"arg1Of","subj":"T9739","obj":"T9737"},{"id":"R8076","pred":"arg1Of","subj":"T9739","obj":"T9738"},{"id":"R8077","pred":"arg1Of","subj":"T9740","obj":"T9741"},{"id":"R8078","pred":"arg2Of","subj":"T9742","obj":"T9741"},{"id":"R8079","pred":"arg1Of","subj":"T9745","obj":"T9744"},{"id":"R8080","pred":"arg2Of","subj":"T9746","obj":"T9740"},{"id":"R8081","pred":"arg2Of","subj":"T9746","obj":"T9742"},{"id":"R8082","pred":"arg1Of","subj":"T9746","obj":"T9743"},{"id":"R8083","pred":"arg1Of","subj":"T9746","obj":"T9745"},{"id":"R8084","pred":"arg1Of","subj":"T9749","obj":"T9747"},{"id":"R8085","pred":"arg1Of","subj":"T9749","obj":"T9748"},{"id":"R8086","pred":"arg1Of","subj":"T9749","obj":"T9750"},{"id":"R8087","pred":"arg1Of","subj":"T9749","obj":"T9753"},{"id":"R8088","pred":"arg2Of","subj":"T9749","obj":"T9754"},{"id":"R8089","pred":"arg2Of","subj":"T9752","obj":"T9750"},{"id":"R8090","pred":"arg1Of","subj":"T9752","obj":"T9751"},{"id":"R8091","pred":"arg2Of","subj":"T9754","obj":"T9753"},{"id":"R8092","pred":"arg1Of","subj":"T9754","obj":"T9755"},{"id":"R8093","pred":"arg2Of","subj":"T9757","obj":"T9755"},{"id":"R8094","pred":"arg1Of","subj":"T9757","obj":"T9756"},{"id":"R8095","pred":"arg1Of","subj":"T9757","obj":"T9758"},{"id":"R8096","pred":"arg2Of","subj":"T9760","obj":"T9758"},{"id":"R8097","pred":"arg1Of","subj":"T9760","obj":"T9759"},{"id":"R8098","pred":"arg1Of","subj":"T9760","obj":"T9761"},{"id":"R8099","pred":"arg2Of","subj":"T9763","obj":"T9761"},{"id":"R8100","pred":"arg1Of","subj":"T9763","obj":"T9762"},{"id":"R8101","pred":"arg3Of","subj":"T9764","obj":"T9761"},{"id":"R8102","pred":"arg1Of","subj":"T9767","obj":"T9768"},{"id":"R8103","pred":"arg1Of","subj":"T9767","obj":"T9773"},{"id":"R8104","pred":"arg2Of","subj":"T9771","obj":"T9768"},{"id":"R8105","pred":"arg1Of","subj":"T9771","obj":"T9769"},{"id":"R8106","pred":"arg1Of","subj":"T9771","obj":"T9770"},{"id":"R8107","pred":"arg1Of","subj":"T9773","obj":"T9765"},{"id":"R8108","pred":"arg1Of","subj":"T9773","obj":"T9766"},{"id":"R8109","pred":"arg1Of","subj":"T9773","obj":"T9772"},{"id":"R8110","pred":"arg1Of","subj":"T9773","obj":"T9780"},{"id":"R8111","pred":"arg2Of","subj":"T9775","obj":"T9773"},{"id":"R8112","pred":"arg1Of","subj":"T9775","obj":"T9774"},{"id":"R8113","pred":"arg1Of","subj":"T9775","obj":"T9776"},{"id":"R8114","pred":"arg2Of","subj":"T9779","obj":"T9776"},{"id":"R8115","pred":"arg1Of","subj":"T9779","obj":"T9777"},{"id":"R8116","pred":"arg1Of","subj":"T9779","obj":"T9778"},{"id":"R8117","pred":"arg2Of","subj":"T9782","obj":"T9780"},{"id":"R8118","pred":"arg1Of","subj":"T9782","obj":"T9781"},{"id":"R8119","pred":"arg1Of","subj":"T9782","obj":"T9783"},{"id":"R8120","pred":"arg2Of","subj":"T9785","obj":"T9783"},{"id":"R8121","pred":"arg1Of","subj":"T9785","obj":"T9784"},{"id":"R8122","pred":"arg3Of","subj":"T9786","obj":"T9783"},{"id":"R8123","pred":"arg1Of","subj":"T9789","obj":"T9787"},{"id":"R8124","pred":"arg1Of","subj":"T9789","obj":"T9788"},{"id":"R8125","pred":"arg1Of","subj":"T9789","obj":"T9790"},{"id":"R8126","pred":"arg1Of","subj":"T9789","obj":"T9793"},{"id":"R8127","pred":"arg1Of","subj":"T9789","obj":"T9795"},{"id":"R8128","pred":"arg2Of","subj":"T9792","obj":"T9790"},{"id":"R8129","pred":"arg1Of","subj":"T9792","obj":"T9791"},{"id":"R8130","pred":"arg1Of","subj":"T9793","obj":"T9794"},{"id":"R8131","pred":"arg1Of","subj":"T9793","obj":"T9799"},{"id":"R8132","pred":"arg1Of","subj":"T9793","obj":"T9800"},{"id":"R8133","pred":"arg2Of","subj":"T9795","obj":"T9793"},{"id":"R8134","pred":"arg1Of","subj":"T9795","obj":"T9796"},{"id":"R8135","pred":"arg2Of","subj":"T9798","obj":"T9796"},{"id":"R8136","pred":"arg1Of","subj":"T9798","obj":"T9797"},{"id":"R8137","pred":"arg1Of","subj":"T9802","obj":"T9801"},{"id":"R8138","pred":"arg1Of","subj":"T9803","obj":"T9802"},{"id":"R8139","pred":"arg1Of","subj":"T9803","obj":"T9804"},{"id":"R8140","pred":"arg2Of","subj":"T9804","obj":"T9800"},{"id":"R8141","pred":"arg1Of","subj":"T9804","obj":"T9805"},{"id":"R8142","pred":"arg1Of","subj":"T9807","obj":"T9808"},{"id":"R8143","pred":"arg2Of","subj":"T9809","obj":"T9805"},{"id":"R8144","pred":"arg1Of","subj":"T9809","obj":"T9806"},{"id":"R8145","pred":"arg1Of","subj":"T9809","obj":"T9807"},{"id":"R8146","pred":"arg1Of","subj":"T9809","obj":"T9810"},{"id":"R8147","pred":"arg1Of","subj":"T9809","obj":"T9813"},{"id":"R8148","pred":"arg2Of","subj":"T9812","obj":"T9810"},{"id":"R8149","pred":"arg1Of","subj":"T9812","obj":"T9811"},{"id":"R8150","pred":"arg2Of","subj":"T9816","obj":"T9813"},{"id":"R8151","pred":"arg1Of","subj":"T9816","obj":"T9814"},{"id":"R8152","pred":"arg1Of","subj":"T9816","obj":"T9815"},{"id":"R8153","pred":"arg1Of","subj":"T9816","obj":"T9817"},{"id":"R8154","pred":"arg2Of","subj":"T9819","obj":"T9817"},{"id":"R8155","pred":"arg1Of","subj":"T9819","obj":"T9818"},{"id":"R8156","pred":"arg3Of","subj":"T9820","obj":"T9817"},{"id":"R8157","pred":"arg2Of","subj":"T9822","obj":"T9821"},{"id":"R8158","pred":"arg1Of","subj":"T9825","obj":"T9824"},{"id":"R8159","pred":"arg1Of","subj":"T9825","obj":"T9826"},{"id":"R8160","pred":"arg1Of","subj":"T9825","obj":"T9830"},{"id":"R8161","pred":"arg1Of","subj":"T9825","obj":"T9843"},{"id":"R8162","pred":"arg2Of","subj":"T9829","obj":"T9826"},{"id":"R8163","pred":"arg1Of","subj":"T9829","obj":"T9827"},{"id":"R8164","pred":"arg1Of","subj":"T9829","obj":"T9828"},{"id":"R8165","pred":"arg1Of","subj":"T9833","obj":"T9831"},{"id":"R8166","pred":"arg1Of","subj":"T9833","obj":"T9832"},{"id":"R8167","pred":"arg1Of","subj":"T9833","obj":"T9834"},{"id":"R8168","pred":"arg1Of","subj":"T9833","obj":"T9839"},{"id":"R8169","pred":"arg2Of","subj":"T9835","obj":"T9834"},{"id":"R8170","pred":"arg3Of","subj":"T9836","obj":"T9834"},{"id":"R8171","pred":"arg2Of","subj":"T9839","obj":"T9830"},{"id":"R8172","pred":"arg1Of","subj":"T9839","obj":"T9837"},{"id":"R8173","pred":"arg1Of","subj":"T9839","obj":"T9838"},{"id":"R8174","pred":"arg2Of","subj":"T9842","obj":"T9839"},{"id":"R8175","pred":"arg1Of","subj":"T9842","obj":"T9840"},{"id":"R8176","pred":"arg1Of","subj":"T9842","obj":"T9841"},{"id":"R8177","pred":"arg1Of","subj":"T9843","obj":"T9821"},{"id":"R8178","pred":"arg1Of","subj":"T9843","obj":"T9823"},{"id":"R8179","pred":"arg2Of","subj":"T9845","obj":"T9843"},{"id":"R8180","pred":"arg1Of","subj":"T9845","obj":"T9844"},{"id":"R8181","pred":"arg1Of","subj":"T9845","obj":"T9846"},{"id":"R8182","pred":"arg2Of","subj":"T9849","obj":"T9846"},{"id":"R8183","pred":"arg1Of","subj":"T9849","obj":"T9847"},{"id":"R8184","pred":"arg1Of","subj":"T9849","obj":"T9848"},{"id":"R8185","pred":"arg1Of","subj":"T9849","obj":"T9850"},{"id":"R8186","pred":"arg2Of","subj":"T9851","obj":"T9850"},{"id":"R8187","pred":"arg2Of","subj":"T9851","obj":"T9853"},{"id":"R8188","pred":"arg1Of","subj":"T9853","obj":"T9852"},{"id":"R8189","pred":"arg3Of","subj":"T9854","obj":"T9850"},{"id":"R8190","pred":"arg1Of","subj":"T9856","obj":"T9855"},{"id":"R8191","pred":"arg1Of","subj":"T9856","obj":"T9857"},{"id":"R8192","pred":"arg2Of","subj":"T9860","obj":"T9857"},{"id":"R8193","pred":"arg1Of","subj":"T9860","obj":"T9858"},{"id":"R8194","pred":"arg1Of","subj":"T9860","obj":"T9859"},{"id":"R8195","pred":"arg1Of","subj":"T9860","obj":"T9861"},{"id":"R8196","pred":"arg1Of","subj":"T9862","obj":"T9863"},{"id":"R8197","pred":"arg2Of","subj":"T9863","obj":"T9861"},{"id":"R8198","pred":"arg1Of","subj":"T9863","obj":"T9865"},{"id":"R8199","pred":"arg2Of","subj":"T9864","obj":"T9863"},{"id":"R8200","pred":"arg2Of","subj":"T9868","obj":"T9865"},{"id":"R8201","pred":"arg1Of","subj":"T9868","obj":"T9866"},{"id":"R8202","pred":"arg1Of","subj":"T9868","obj":"T9867"}],"namespaces":[{"prefix":"_base","uri":"http://kmcs.nii.ac.jp/enju/"}],"text":"In vitro methylation of an IRF-4 promoter-reporter construct decreases its activity\nTo provide evidence for a direct effect of methylational status on IRF-4 promoter activity we performed reporter gene assays with IRF-4 promoter constructs before and after their in vitro methylation. A complete methylation of these constructs was checked via restriction assays with methylation-sensitive endonucleases (Figure 5A). Intriguingly, methylation of the IRF-4 promoter significantly decreased promoter activity in IRF-4-positive SD-1 cells by 85.0% (Figure 5B). The silencing effect of CpG methylation was not restricted to IRF-4-positive cells, since in vitro methylation led to a 92.9% abrogation of promoter activity in IRF-4-negative Jurkat cells (Figure 5C). In contrast, control methylation of a reporter construct with a different promoter (FasL) as well as an empty vector had no effect on the reporter activity (data not shown). These data proved a direct association between methylation and activity of the IRF-4 promoter."}

{"project":"bionlp-st-ge-2016-spacy-parsed","denotations":[{"id":"T9878","span":{"begin":0,"end":2},"obj":"IN"},{"id":"T9879","span":{"begin":3,"end":8},"obj":"NN"},{"id":"T9880","span":{"begin":9,"end":20},"obj":"NN"},{"id":"T9881","span":{"begin":21,"end":23},"obj":"IN"},{"id":"T9882","span":{"begin":24,"end":26},"obj":"DT"},{"id":"T9883","span":{"begin":27,"end":32},"obj":"JJ"},{"id":"T9884","span":{"begin":33,"end":50},"obj":"NN"},{"id":"T9885","span":{"begin":51,"end":60},"obj":"VBP"},{"id":"T9886","span":{"begin":61,"end":70},"obj":"VBZ"},{"id":"T9887","span":{"begin":71,"end":74},"obj":"PRP$"},{"id":"T9888","span":{"begin":75,"end":83},"obj":"NN"},{"id":"T9889","span":{"begin":84,"end":86},"obj":"TO"},{"id":"T9890","span":{"begin":87,"end":94},"obj":"VB"},{"id":"T9891","span":{"begin":95,"end":103},"obj":"NN"},{"id":"T9892","span":{"begin":104,"end":107},"obj":"IN"},{"id":"T9893","span":{"begin":108,"end":109},"obj":"DT"},{"id":"T9894","span":{"begin":110,"end":116},"obj":"JJ"},{"id":"T9913","span":{"begin":247,"end":250},"obj":"CC"},{"id":"T9914","span":{"begin":251,"end":256},"obj":"IN"},{"id":"T9915","span":{"begin":257,"end":262},"obj":"PRP$"},{"id":"T9916","span":{"begin":263,"end":265},"obj":"IN"},{"id":"T9917","span":{"begin":266,"end":271},"obj":"NN"},{"id":"T9918","span":{"begin":272,"end":283},"obj":"NN"},{"id":"T9919","span":{"begin":283,"end":284},"obj":"."},{"id":"T9920","span":{"begin":285,"end":286},"obj":"DT"},{"id":"T9921","span":{"begin":287,"end":295},"obj":"JJ"},{"id":"T9922","span":{"begin":296,"end":307},"obj":"NN"},{"id":"T9923","span":{"begin":308,"end":310},"obj":"IN"},{"id":"T9924","span":{"begin":311,"end":316},"obj":"DT"},{"id":"T9925","span":{"begin":317,"end":327},"obj":"NNS"},{"id":"T9926","span":{"begin":328,"end":331},"obj":"VBD"},{"id":"T9927","span":{"begin":332,"end":339},"obj":"VBN"},{"id":"T9928","span":{"begin":340,"end":343},"obj":"IN"},{"id":"T9929","span":{"begin":344,"end":355},"obj":"NN"},{"id":"T9930","span":{"begin":356,"end":362},"obj":"NNS"},{"id":"T9931","span":{"begin":363,"end":367},"obj":"IN"},{"id":"T9932","span":{"begin":368,"end":389},"obj":"JJ"},{"id":"T9933","span":{"begin":390,"end":403},"obj":"NNS"},{"id":"T9934","span":{"begin":404,"end":405},"obj":"-LRB-"},{"id":"T9935","span":{"begin":405,"end":411},"obj":"NN"},{"id":"T9936","span":{"begin":412,"end":414},"obj":"NN"},{"id":"T9937","span":{"begin":414,"end":415},"obj":"-RRB-"},{"id":"T9938","span":{"begin":415,"end":416},"obj":"."},{"id":"T9939","span":{"begin":417,"end":429},"obj":"RB"},{"id":"T9940","span":{"begin":429,"end":430},"obj":","},{"id":"T9941","span":{"begin":431,"end":442},"obj":"NN"},{"id":"T9942","span":{"begin":443,"end":445},"obj":"IN"},{"id":"T9943","span":{"begin":446,"end":449},"obj":"DT"},{"id":"T9944","span":{"begin":450,"end":455},"obj":"NN"},{"id":"T9945","span":{"begin":456,"end":464},"obj":"NN"},{"id":"T9946","span":{"begin":465,"end":478},"obj":"RB"},{"id":"T9947","span":{"begin":479,"end":488},"obj":"VBD"},{"id":"T9948","span":{"begin":489,"end":497},"obj":"NN"},{"id":"T9949","span":{"begin":498,"end":506},"obj":"NN"},{"id":"T9950","span":{"begin":507,"end":509},"obj":"IN"},{"id":"T9951","span":{"begin":510,"end":524},"obj":"JJ"},{"id":"T9952","span":{"begin":525,"end":529},"obj":"NN"},{"id":"T9953","span":{"begin":530,"end":535},"obj":"NNS"},{"id":"T9954","span":{"begin":536,"end":538},"obj":"IN"},{"id":"T9955","span":{"begin":539,"end":543},"obj":"CD"},{"id":"T9956","span":{"begin":543,"end":544},"obj":"NN"},{"id":"T9957","span":{"begin":545,"end":546},"obj":"-LRB-"},{"id":"T9958","span":{"begin":546,"end":552},"obj":"NN"},{"id":"T9959","span":{"begin":553,"end":555},"obj":"NN"},{"id":"T9960","span":{"begin":555,"end":556},"obj":"-RRB-"},{"id":"T9961","span":{"begin":556,"end":557},"obj":"."},{"id":"T9962","span":{"begin":558,"end":561},"obj":"DT"},{"id":"T9963","span":{"begin":562,"end":571},"obj":"VBG"},{"id":"T9964","span":{"begin":572,"end":578},"obj":"NN"},{"id":"T9965","span":{"begin":579,"end":581},"obj":"IN"},{"id":"T9966","span":{"begin":582,"end":585},"obj":"NNP"},{"id":"T9967","span":{"begin":586,"end":597},"obj":"NN"},{"id":"T9968","span":{"begin":598,"end":601},"obj":"VBD"},{"id":"T9969","span":{"begin":602,"end":605},"obj":"RB"},{"id":"T9970","span":{"begin":606,"end":616},"obj":"VBN"},{"id":"T9971","span":{"begin":617,"end":619},"obj":"TO"},{"id":"T9972","span":{"begin":620,"end":634},"obj":"JJ"},{"id":"T9973","span":{"begin":635,"end":640},"obj":"NNS"},{"id":"T9974","span":{"begin":640,"end":641},"obj":","},{"id":"T9975","span":{"begin":642,"end":647},"obj":"IN"},{"id":"T9976","span":{"begin":648,"end":650},"obj":"IN"},{"id":"T9977","span":{"begin":651,"end":656},"obj":"NN"},{"id":"T9978","span":{"begin":657,"end":668},"obj":"NN"},{"id":"T9979","span":{"begin":669,"end":672},"obj":"VBD"},{"id":"T9980","span":{"begin":673,"end":675},"obj":"TO"},{"id":"T9981","span":{"begin":676,"end":677},"obj":"DT"},{"id":"T9982","span":{"begin":678,"end":682},"obj":"CD"},{"id":"T9983","span":{"begin":682,"end":683},"obj":"NN"},{"id":"T9984","span":{"begin":684,"end":694},"obj":"NN"},{"id":"T9985","span":{"begin":695,"end":697},"obj":"IN"},{"id":"T9986","span":{"begin":698,"end":706},"obj":"NN"},{"id":"T9987","span":{"begin":707,"end":715},"obj":"NN"},{"id":"T9988","span":{"begin":716,"end":718},"obj":"IN"},{"id":"T9989","span":{"begin":719,"end":733},"obj":"JJ"},{"id":"T9990","span":{"begin":734,"end":740},"obj":"NNP"},{"id":"T9991","span":{"begin":741,"end":746},"obj":"NNS"},{"id":"T9992","span":{"begin":747,"end":748},"obj":"-LRB-"},{"id":"T9993","span":{"begin":748,"end":754},"obj":"NN"},{"id":"T9994","span":{"begin":755,"end":757},"obj":"NN"},{"id":"T9995","span":{"begin":757,"end":758},"obj":"-RRB-"},{"id":"T9996","span":{"begin":758,"end":759},"obj":"."},{"id":"T9997","span":{"begin":760,"end":762},"obj":"IN"},{"id":"T9998","span":{"begin":763,"end":771},"obj":"NN"},{"id":"T9999","span":{"begin":771,"end":772},"obj":","},{"id":"T10000","span":{"begin":773,"end":780},"obj":"NN"},{"id":"T10001","span":{"begin":781,"end":792},"obj":"NN"},{"id":"T10002","span":{"begin":793,"end":795},"obj":"IN"},{"id":"T10003","span":{"begin":796,"end":797},"obj":"DT"},{"id":"T10004","span":{"begin":798,"end":806},"obj":"NN"},{"id":"T10005","span":{"begin":807,"end":816},"obj":"VBP"},{"id":"T10006","span":{"begin":817,"end":821},"obj":"IN"},{"id":"T10007","span":{"begin":822,"end":823},"obj":"DT"},{"id":"T10008","span":{"begin":824,"end":833},"obj":"JJ"},{"id":"T10009","span":{"begin":834,"end":842},"obj":"NN"},{"id":"T10010","span":{"begin":843,"end":844},"obj":"-LRB-"},{"id":"T10011","span":{"begin":844,"end":848},"obj":"NNP"},{"id":"T10012","span":{"begin":848,"end":849},"obj":"-RRB-"},{"id":"T10013","span":{"begin":850,"end":852},"obj":"RB"},{"id":"T10014","span":{"begin":853,"end":857},"obj":"RB"},{"id":"T10015","span":{"begin":858,"end":860},"obj":"IN"},{"id":"T10016","span":{"begin":861,"end":863},"obj":"DT"},{"id":"T10017","span":{"begin":864,"end":869},"obj":"JJ"},{"id":"T10018","span":{"begin":870,"end":876},"obj":"NN"},{"id":"T10019","span":{"begin":877,"end":880},"obj":"VBD"},{"id":"T10020","span":{"begin":881,"end":883},"obj":"DT"},{"id":"T10021","span":{"begin":884,"end":890},"obj":"NN"},{"id":"T10022","span":{"begin":891,"end":893},"obj":"IN"},{"id":"T10023","span":{"begin":894,"end":897},"obj":"DT"},{"id":"T10024","span":{"begin":898,"end":906},"obj":"NN"},{"id":"T10025","span":{"begin":907,"end":915},"obj":"NN"},{"id":"T10026","span":{"begin":916,"end":917},"obj":"-LRB-"},{"id":"T10027","span":{"begin":917,"end":921},"obj":"NNS"},{"id":"T10028","span":{"begin":922,"end":925},"obj":"RB"},{"id":"T10029","span":{"begin":926,"end":931},"obj":"VBN"},{"id":"T10030","span":{"begin":931,"end":932},"obj":"-RRB-"},{"id":"T10031","span":{"begin":932,"end":933},"obj":"."},{"id":"T10032","span":{"begin":934,"end":939},"obj":"DT"},{"id":"T10033","span":{"begin":940,"end":944},"obj":"NNS"},{"id":"T10034","span":{"begin":945,"end":951},"obj":"VBD"},{"id":"T10035","span":{"begin":952,"end":953},"obj":"DT"},{"id":"T10036","span":{"begin":954,"end":960},"obj":"JJ"},{"id":"T10037","span":{"begin":961,"end":972},"obj":"NN"},{"id":"T10038","span":{"begin":973,"end":980},"obj":"IN"},{"id":"T10039","span":{"begin":981,"end":992},"obj":"NN"},{"id":"T10040","span":{"begin":993,"end":996},"obj":"CC"},{"id":"T10041","span":{"begin":997,"end":1005},"obj":"NN"},{"id":"T10042","span":{"begin":1006,"end":1008},"obj":"IN"},{"id":"T10043","span":{"begin":1009,"end":1012},"obj":"DT"},{"id":"T10044","span":{"begin":1013,"end":1018},"obj":"NN"},{"id":"T10045","span":{"begin":1019,"end":1027},"obj":"NN"},{"id":"T10046","span":{"begin":1027,"end":1028},"obj":"."},{"id":"T9895","span":{"begin":117,"end":123},"obj":"NN"},{"id":"T9896","span":{"begin":124,"end":126},"obj":"IN"},{"id":"T9897","span":{"begin":127,"end":140},"obj":"JJ"},{"id":"T9898","span":{"begin":141,"end":147},"obj":"NN"},{"id":"T9899","span":{"begin":148,"end":150},"obj":"IN"},{"id":"T9900","span":{"begin":151,"end":156},"obj":"NN"},{"id":"T9901","span":{"begin":157,"end":165},"obj":"NN"},{"id":"T9902","span":{"begin":166,"end":174},"obj":"NN"},{"id":"T9903","span":{"begin":175,"end":177},"obj":"PRP"},{"id":"T9904","span":{"begin":178,"end":187},"obj":"VBD"},{"id":"T9905","span":{"begin":188,"end":196},"obj":"NN"},{"id":"T9906","span":{"begin":197,"end":201},"obj":"NN"},{"id":"T9907","span":{"begin":202,"end":208},"obj":"NNS"},{"id":"T9908","span":{"begin":209,"end":213},"obj":"IN"},{"id":"T9909","span":{"begin":214,"end":219},"obj":"JJ"},{"id":"T9910","span":{"begin":220,"end":228},"obj":"NN"},{"id":"T9911","span":{"begin":229,"end":239},"obj":"NNS"},{"id":"T9912","span":{"begin":240,"end":246},"obj":"IN"}],"relations":[{"id":"R8203","pred":"prep","subj":"T9878","obj":"T9886"},{"id":"R8204","pred":"amod","subj":"T9879","obj":"T9880"},{"id":"R8205","pred":"pobj","subj":"T9880","obj":"T9878"},{"id":"R8206","pred":"prep","subj":"T9881","obj":"T9880"},{"id":"R8207","pred":"det","subj":"T9882","obj":"T9884"},{"id":"R8208","pred":"amod","subj":"T9883","obj":"T9884"},{"id":"R8209","pred":"pobj","subj":"T9884","obj":"T9881"},{"id":"R8210","pred":"nsubj","subj":"T9885","obj":"T9886"},{"id":"R8211","pred":"ROOT","subj":"T9886","obj":"T9886"},{"id":"R8212","pred":"poss","subj":"T9887","obj":"T9888"},{"id":"R8213","pred":"dobj","subj":"T9888","obj":"T9886"},{"id":"R8214","pred":"aux","subj":"T9889","obj":"T9890"},{"id":"R8215","pred":"advcl","subj":"T9890","obj":"T9886"},{"id":"R8216","pred":"dobj","subj":"T9891","obj":"T9890"},{"id":"R8217","pred":"prep","subj":"T9892","obj":"T9891"},{"id":"R8218","pred":"det","subj":"T9893","obj":"T9895"},{"id":"R8219","pred":"amod","subj":"T9894","obj":"T9895"},{"id":"R8220","pred":"pobj","subj":"T9895","obj":"T9892"},{"id":"R8221","pred":"prep","subj":"T9896","obj":"T9895"},{"id":"R8222","pred":"amod","subj":"T9897","obj":"T9898"},{"id":"R8223","pred":"pobj","subj":"T9898","obj":"T9896"},{"id":"R8224","pred":"prep","subj":"T9899","obj":"T9898"},{"id":"R8225","pred":"compound","subj":"T9900","obj":"T9902"},{"id":"R8226","pred":"compound","subj":"T9901","obj":"T9902"},{"id":"R8227","pred":"pobj","subj":"T9902","obj":"T9899"},{"id":"R8228","pred":"nsubj","subj":"T9903","obj":"T9904"},{"id":"R8229","pred":"ROOT","subj":"T9904","obj":"T9904"},{"id":"R8230","pred":"compound","subj":"T9905","obj":"T9907"},{"id":"R8231","pred":"compound","subj":"T9906","obj":"T9907"},{"id":"R8232","pred":"dobj","subj":"T9907","obj":"T9904"},{"id":"R8233","pred":"prep","subj":"T9908","obj":"T9907"},{"id":"R8234","pred":"amod","subj":"T9909","obj":"T9911"},{"id":"R8235","pred":"compound","subj":"T9910","obj":"T9911"},{"id":"R8236","pred":"pobj","subj":"T9911","obj":"T9908"},{"id":"R8237","pred":"advmod","subj":"T9912","obj":"T9904"},{"id":"R8238","pred":"cc","subj":"T9913","obj":"T9912"},{"id":"R8239","pred":"conj","subj":"T9914","obj":"T9912"},{"id":"R8240","pred":"poss","subj":"T9915","obj":"T9916"},{"id":"R8241","pred":"prep","subj":"T9916","obj":"T9914"},{"id":"R8242","pred":"amod","subj":"T9917","obj":"T9918"},{"id":"R8243","pred":"pobj","subj":"T9918","obj":"T9916"},{"id":"R8244","pred":"punct","subj":"T9919","obj":"T9904"},{"id":"R8245","pred":"det","subj":"T9920","obj":"T9922"},{"id":"R8246","pred":"amod","subj":"T9921","obj":"T9922"},{"id":"R8247","pred":"nsubjpass","subj":"T9922","obj":"T9927"},{"id":"R8248","pred":"prep","subj":"T9923","obj":"T9922"},{"id":"R8249","pred":"det","subj":"T9924","obj":"T9925"},{"id":"R8250","pred":"pobj","subj":"T9925","obj":"T9923"},{"id":"R8251","pred":"auxpass","subj":"T9926","obj":"T9927"},{"id":"R8252","pred":"ROOT","subj":"T9927","obj":"T9927"},{"id":"R8253","pred":"prep","subj":"T9928","obj":"T9927"},{"id":"R8254","pred":"compound","subj":"T9929","obj":"T9930"},{"id":"R8255","pred":"pobj","subj":"T9930","obj":"T9928"},{"id":"R8256","pred":"prep","subj":"T9931","obj":"T9930"},{"id":"R8257","pred":"amod","subj":"T9932","obj":"T9933"},{"id":"R8258","pred":"pobj","subj":"T9933","obj":"T9931"},{"id":"R8259","pred":"punct","subj":"T9934","obj":"T9936"},{"id":"R8260","pred":"compound","subj":"T9935","obj":"T9936"},{"id":"R8261","pred":"appos","subj":"T9936","obj":"T9933"},{"id":"R8262","pred":"punct","subj":"T9937","obj":"T9936"},{"id":"R8263","pred":"punct","subj":"T9938","obj":"T9927"},{"id":"R8264","pred":"advmod","subj":"T9939","obj":"T9947"},{"id":"R8265","pred":"punct","subj":"T9940","obj":"T9947"},{"id":"R8266","pred":"nsubj","subj":"T9941","obj":"T9947"},{"id":"R8267","pred":"prep","subj":"T9942","obj":"T9941"},{"id":"R8268","pred":"det","subj":"T9943","obj":"T9945"},{"id":"R8269","pred":"compound","subj":"T9944","obj":"T9945"},{"id":"R8270","pred":"pobj","subj":"T9945","obj":"T9942"},{"id":"R8271","pred":"advmod","subj":"T9946","obj":"T9947"},{"id":"R8272","pred":"ROOT","subj":"T9947","obj":"T9947"},{"id":"R8273","pred":"compound","subj":"T9948","obj":"T9949"},{"id":"R8274","pred":"dobj","subj":"T9949","obj":"T9947"},{"id":"R8275","pred":"prep","subj":"T9950","obj":"T9949"},{"id":"R8276","pred":"amod","subj":"T9951","obj":"T9953"},{"id":"R8277","pred":"compound","subj":"T9952","obj":"T9953"},{"id":"R8278","pred":"pobj","subj":"T9953","obj":"T9950"},{"id":"R8279","pred":"prep","subj":"T9954","obj":"T9947"},{"id":"R8280","pred":"nummod","subj":"T9955","obj":"T9956"},{"id":"R8281","pred":"pobj","subj":"T9956","obj":"T9954"},{"id":"R8282","pred":"punct","subj":"T9957","obj":"T9959"},{"id":"R8283","pred":"compound","subj":"T9958","obj":"T9959"},{"id":"R8284","pred":"appos","subj":"T9959","obj":"T9956"},{"id":"R8285","pred":"punct","subj":"T9960","obj":"T9959"},{"id":"R8286","pred":"punct","subj":"T9961","obj":"T9947"},{"id":"R8287","pred":"det","subj":"T9962","obj":"T9964"},{"id":"R8288","pred":"amod","subj":"T9963","obj":"T9964"},{"id":"R8289","pred":"nsubjpass","subj":"T9964","obj":"T9970"},{"id":"R8290","pred":"prep","subj":"T9965","obj":"T9964"},{"id":"R8291","pred":"compound","subj":"T9966","obj":"T9967"},{"id":"R8292","pred":"pobj","subj":"T9967","obj":"T9965"},{"id":"R8293","pred":"auxpass","subj":"T9968","obj":"T9970"},{"id":"R8294","pred":"neg","subj":"T9969","obj":"T9970"},{"id":"R8295","pred":"ROOT","subj":"T9970","obj":"T9970"},{"id":"R8296","pred":"prep","subj":"T9971","obj":"T9970"},{"id":"R8297","pred":"amod","subj":"T9972","obj":"T9973"},{"id":"R8298","pred":"pobj","subj":"T9973","obj":"T9971"},{"id":"R8299","pred":"punct","subj":"T9974","obj":"T9970"},{"id":"R8300","pred":"mark","subj":"T9975","obj":"T9979"},{"id":"R8301","pred":"prep","subj":"T9976","obj":"T9979"},{"id":"R8302","pred":"amod","subj":"T9977","obj":"T9978"},{"id":"R8303","pred":"pobj","subj":"T9978","obj":"T9976"},{"id":"R8304","pred":"advcl","subj":"T9979","obj":"T9970"},{"id":"R8305","pred":"prep","subj":"T9980","obj":"T9979"},{"id":"R8306","pred":"det","subj":"T9981","obj":"T9984"},{"id":"R8307","pred":"nummod","subj":"T9982","obj":"T9983"},{"id":"R8308","pred":"compound","subj":"T9983","obj":"T9984"},{"id":"R8309","pred":"pobj","subj":"T9984","obj":"T9980"},{"id":"R8310","pred":"prep","subj":"T9985","obj":"T9984"},{"id":"R8311","pred":"compound","subj":"T9986","obj":"T9987"},{"id":"R8312","pred":"pobj","subj":"T9987","obj":"T9985"},{"id":"R8313","pred":"prep","subj":"T9988","obj":"T9987"},{"id":"R8314","pred":"amod","subj":"T9989","obj":"T9991"},{"id":"R8315","pred":"compound","subj":"T9990","obj":"T9991"},{"id":"R8316","pred":"pobj","subj":"T9991","obj":"T9988"},{"id":"R8317","pred":"punct","subj":"T9992","obj":"T9994"},{"id":"R8318","pred":"compound","subj":"T9993","obj":"T9994"},{"id":"R8319","pred":"appos","subj":"T9994","obj":"T9987"},{"id":"R8320","pred":"punct","subj":"T9995","obj":"T9994"},{"id":"R8321","pred":"punct","subj":"T9996","obj":"T9970"},{"id":"R8322","pred":"prep","subj":"T9997","obj":"T10005"},{"id":"R8323","pred":"pobj","subj":"T9998","obj":"T9997"},{"id":"R8324","pred":"punct","subj":"T9999","obj":"T10005"},{"id":"R8325","pred":"nsubj","subj":"T10000","obj":"T10005"},{"id":"R8326","pred":"dobj","subj":"T10001","obj":"T10000"},{"id":"R8327","pred":"prep","subj":"T10002","obj":"T10000"},{"id":"R8328","pred":"det","subj":"T10003","obj":"T10004"},{"id":"R8329","pred":"pobj","subj":"T10004","obj":"T10002"},{"id":"R8330","pred":"ROOT","subj":"T10005","obj":"T10005"},{"id":"R8331","pred":"prep","subj":"T10006","obj":"T10005"},{"id":"R8332","pred":"det","subj":"T10007","obj":"T10009"},{"id":"R8333","pred":"amod","subj":"T10008","obj":"T10009"},{"id":"R8334","pred":"pobj","subj":"T10009","obj":"T10006"},{"id":"R8335","pred":"punct","subj":"T10010","obj":"T10009"},{"id":"R8336","pred":"appos","subj":"T10011","obj":"T10009"},{"id":"R8337","pred":"punct","subj":"T10012","obj":"T10009"},{"id":"R8338","pred":"advmod","subj":"T10013","obj":"T10015"},{"id":"R8339","pred":"advmod","subj":"T10014","obj":"T10015"},{"id":"R8340","pred":"cc","subj":"T10015","obj":"T10009"},{"id":"R8341","pred":"det","subj":"T10016","obj":"T10018"},{"id":"R8342","pred":"amod","subj":"T10017","obj":"T10018"},{"id":"R8343","pred":"conj","subj":"T10018","obj":"T10009"},{"id":"R8344","pred":"conj","subj":"T10019","obj":"T10005"},{"id":"R8345","pred":"det","subj":"T10020","obj":"T10021"},{"id":"R8346","pred":"dobj","subj":"T10021","obj":"T10019"},{"id":"R8347","pred":"prep","subj":"T10022","obj":"T10021"},{"id":"R8348","pred":"det","subj":"T10023","obj":"T10025"},{"id":"R8349","pred":"compound","subj":"T10024","obj":"T10025"},{"id":"R8350","pred":"pobj","subj":"T10025","obj":"T10022"},{"id":"R8351","pred":"punct","subj":"T10026","obj":"T10027"},{"id":"R8352","pred":"appos","subj":"T10027","obj":"T10025"},{"id":"R8353","pred":"neg","subj":"T10028","obj":"T10029"},{"id":"R8354","pred":"acl","subj":"T10029","obj":"T10027"},{"id":"R8355","pred":"punct","subj":"T10030","obj":"T10027"},{"id":"R8356","pred":"punct","subj":"T10031","obj":"T10005"},{"id":"R8357","pred":"det","subj":"T10032","obj":"T10033"},{"id":"R8358","pred":"nsubj","subj":"T10033","obj":"T10034"},{"id":"R8359","pred":"ROOT","subj":"T10034","obj":"T10034"},{"id":"R8360","pred":"det","subj":"T10035","obj":"T10037"},{"id":"R8361","pred":"amod","subj":"T10036","obj":"T10037"},{"id":"R8362","pred":"dobj","subj":"T10037","obj":"T10034"},{"id":"R8363","pred":"prep","subj":"T10038","obj":"T10037"},{"id":"R8364","pred":"pobj","subj":"T10039","obj":"T10038"},{"id":"R8365","pred":"cc","subj":"T10040","obj":"T10039"},{"id":"R8366","pred":"conj","subj":"T10041","obj":"T10039"},{"id":"R8367","pred":"prep","subj":"T10042","obj":"T10039"},{"id":"R8368","pred":"det","subj":"T10043","obj":"T10045"},{"id":"R8369","pred":"compound","subj":"T10044","obj":"T10045"},{"id":"R8370","pred":"pobj","subj":"T10045","obj":"T10042"},{"id":"R8371","pred":"punct","subj":"T10046","obj":"T10034"}],"text":"In vitro methylation of an IRF-4 promoter-reporter construct decreases its activity\nTo provide evidence for a direct effect of methylational status on IRF-4 promoter activity we performed reporter gene assays with IRF-4 promoter constructs before and after their in vitro methylation. A complete methylation of these constructs was checked via restriction assays with methylation-sensitive endonucleases (Figure 5A). Intriguingly, methylation of the IRF-4 promoter significantly decreased promoter activity in IRF-4-positive SD-1 cells by 85.0% (Figure 5B). The silencing effect of CpG methylation was not restricted to IRF-4-positive cells, since in vitro methylation led to a 92.9% abrogation of promoter activity in IRF-4-negative Jurkat cells (Figure 5C). In contrast, control methylation of a reporter construct with a different promoter (FasL) as well as an empty vector had no effect on the reporter activity (data not shown). These data proved a direct association between methylation and activity of the IRF-4 promoter."}

{"project":"GO-BP","denotations":[{"id":"T9697","span":{"begin":9,"end":20},"obj":"http://purl.obolibrary.org/obo/GO_0032259"},{"id":"T9698","span":{"begin":272,"end":283},"obj":"http://purl.obolibrary.org/obo/GO_0032259"},{"id":"T9699","span":{"begin":296,"end":307},"obj":"http://purl.obolibrary.org/obo/GO_0032259"},{"id":"T9700","span":{"begin":368,"end":379},"obj":"http://purl.obolibrary.org/obo/GO_0032259"},{"id":"T9701","span":{"begin":431,"end":442},"obj":"http://purl.obolibrary.org/obo/GO_0032259"},{"id":"T9702","span":{"begin":586,"end":597},"obj":"http://purl.obolibrary.org/obo/GO_0032259"},{"id":"T9703","span":{"begin":657,"end":668},"obj":"http://purl.obolibrary.org/obo/GO_0032259"},{"id":"T9704","span":{"begin":781,"end":792},"obj":"http://purl.obolibrary.org/obo/GO_0032259"},{"id":"T9705","span":{"begin":981,"end":992},"obj":"http://purl.obolibrary.org/obo/GO_0032259"}],"text":"In vitro methylation of an IRF-4 promoter-reporter construct decreases its activity\nTo provide evidence for a direct effect of methylational status on IRF-4 promoter activity we performed reporter gene assays with IRF-4 promoter constructs before and after their in vitro methylation. A complete methylation of these constructs was checked via restriction assays with methylation-sensitive endonucleases (Figure 5A). Intriguingly, methylation of the IRF-4 promoter significantly decreased promoter activity in IRF-4-positive SD-1 cells by 85.0% (Figure 5B). The silencing effect of CpG methylation was not restricted to IRF-4-positive cells, since in vitro methylation led to a 92.9% abrogation of promoter activity in IRF-4-negative Jurkat cells (Figure 5C). In contrast, control methylation of a reporter construct with a different promoter (FasL) as well as an empty vector had no effect on the reporter activity (data not shown). These data proved a direct association between methylation and activity of the IRF-4 promoter."}

{"project":"bionlp-st-ge-2016-reference-tees","denotations":[{"id":"T10121","span":{"begin":27,"end":60},"obj":"Protein"},{"id":"T10122","span":{"begin":61,"end":70},"obj":"Negative_regulation"},{"id":"T10123","span":{"begin":151,"end":165},"obj":"Protein"},{"id":"T10124","span":{"begin":450,"end":464},"obj":"Protein"},{"id":"T10125","span":{"begin":510,"end":515},"obj":"Protein"},{"id":"T10126","span":{"begin":489,"end":497},"obj":"Entity"},{"id":"T10127","span":{"begin":516,"end":524},"obj":"Gene_expression"},{"id":"T10128","span":{"begin":620,"end":625},"obj":"Protein"},{"id":"T10129","span":{"begin":719,"end":724},"obj":"Protein"},{"id":"T10130","span":{"begin":626,"end":634},"obj":"Gene_expression"},{"id":"T10131","span":{"begin":698,"end":706},"obj":"Entity"},{"id":"T10132","span":{"begin":725,"end":733},"obj":"Gene_expression"},{"id":"T10133","span":{"begin":844,"end":848},"obj":"Protein"},{"id":"T10134","span":{"begin":1013,"end":1027},"obj":"Protein"}],"relations":[{"id":"R8400","pred":"themeOf","subj":"T10121","obj":"T10122"},{"id":"R8401","pred":"themeOf","subj":"T10125","obj":"T10127"},{"id":"R8402","pred":"themeOf","subj":"T10128","obj":"T10130"},{"id":"R8403","pred":"themeOf","subj":"T10129","obj":"T10132"}],"text":"In vitro methylation of an IRF-4 promoter-reporter construct decreases its activity\nTo provide evidence for a direct effect of methylational status on IRF-4 promoter activity we performed reporter gene assays with IRF-4 promoter constructs before and after their in vitro methylation. A complete methylation of these constructs was checked via restriction assays with methylation-sensitive endonucleases (Figure 5A). Intriguingly, methylation of the IRF-4 promoter significantly decreased promoter activity in IRF-4-positive SD-1 cells by 85.0% (Figure 5B). The silencing effect of CpG methylation was not restricted to IRF-4-positive cells, since in vitro methylation led to a 92.9% abrogation of promoter activity in IRF-4-negative Jurkat cells (Figure 5C). In contrast, control methylation of a reporter construct with a different promoter (FasL) as well as an empty vector had no effect on the reporter activity (data not shown). These data proved a direct association between methylation and activity of the IRF-4 promoter."}

{"project":"bionlp-st-ge-2016-reference","denotations":[{"id":"T9682","span":{"begin":620,"end":625},"obj":"Protein"},{"id":"T9683","span":{"begin":626,"end":634},"obj":"Gene_expression"},{"id":"T9684","span":{"begin":684,"end":694},"obj":"Negative_regulation"},{"id":"T9685","span":{"begin":698,"end":706},"obj":"Entity"},{"id":"T9686","span":{"begin":719,"end":724},"obj":"Protein"},{"id":"T9687","span":{"begin":725,"end":733},"obj":"Gene_expression"},{"id":"T9688","span":{"begin":844,"end":848},"obj":"Protein"},{"id":"T9689","span":{"begin":1013,"end":1018},"obj":"Protein"},{"id":"T9674","span":{"begin":27,"end":32},"obj":"Protein"},{"id":"T9675","span":{"begin":151,"end":156},"obj":"Protein"},{"id":"T9676","span":{"begin":214,"end":219},"obj":"Protein"},{"id":"T9677","span":{"begin":450,"end":455},"obj":"Protein"},{"id":"T9678","span":{"begin":479,"end":488},"obj":"Negative_regulation"},{"id":"T9679","span":{"begin":489,"end":497},"obj":"Entity"},{"id":"T9680","span":{"begin":510,"end":515},"obj":"Protein"},{"id":"T9681","span":{"begin":516,"end":524},"obj":"Gene_expression"}],"relations":[{"id":"R8035","pred":"themeOf","subj":"T9679","obj":"T9678"},{"id":"R8036","pred":"partOf","subj":"T9679","obj":"T9677"},{"id":"R8037","pred":"themeOf","subj":"T9680","obj":"T9681"},{"id":"R8038","pred":"themeOf","subj":"T9682","obj":"T9683"},{"id":"R8039","pred":"themeOf","subj":"T9685","obj":"T9684"},{"id":"R8040","pred":"partOf","subj":"T9685","obj":"T9686"},{"id":"R8041","pred":"themeOf","subj":"T9686","obj":"T9687"}],"attributes":[{"id":"M213","pred":"Negation","subj":"T9687","obj":"true"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"In vitro methylation of an IRF-4 promoter-reporter construct decreases its activity\nTo provide evidence for a direct effect of methylational status on IRF-4 promoter activity we performed reporter gene assays with IRF-4 promoter constructs before and after their in vitro methylation. A complete methylation of these constructs was checked via restriction assays with methylation-sensitive endonucleases (Figure 5A). Intriguingly, methylation of the IRF-4 promoter significantly decreased promoter activity in IRF-4-positive SD-1 cells by 85.0% (Figure 5B). The silencing effect of CpG methylation was not restricted to IRF-4-positive cells, since in vitro methylation led to a 92.9% abrogation of promoter activity in IRF-4-negative Jurkat cells (Figure 5C). In contrast, control methylation of a reporter construct with a different promoter (FasL) as well as an empty vector had no effect on the reporter activity (data not shown). These data proved a direct association between methylation and activity of the IRF-4 promoter."}

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T9869","span":{"begin":27,"end":32},"obj":"Q15306"},{"id":"T9870","span":{"begin":151,"end":156},"obj":"Q15306"},{"id":"T9871","span":{"begin":214,"end":219},"obj":"Q15306"},{"id":"T9872","span":{"begin":450,"end":455},"obj":"Q15306"},{"id":"T9873","span":{"begin":510,"end":515},"obj":"Q15306"},{"id":"T9874","span":{"begin":620,"end":625},"obj":"Q15306"},{"id":"T9875","span":{"begin":719,"end":724},"obj":"Q15306"},{"id":"T9876","span":{"begin":844,"end":848},"obj":"P48023"},{"id":"T9877","span":{"begin":1013,"end":1018},"obj":"Q15306"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"In vitro methylation of an IRF-4 promoter-reporter construct decreases its activity\nTo provide evidence for a direct effect of methylational status on IRF-4 promoter activity we performed reporter gene assays with IRF-4 promoter constructs before and after their in vitro methylation. A complete methylation of these constructs was checked via restriction assays with methylation-sensitive endonucleases (Figure 5A). Intriguingly, methylation of the IRF-4 promoter significantly decreased promoter activity in IRF-4-positive SD-1 cells by 85.0% (Figure 5B). The silencing effect of CpG methylation was not restricted to IRF-4-positive cells, since in vitro methylation led to a 92.9% abrogation of promoter activity in IRF-4-negative Jurkat cells (Figure 5C). In contrast, control methylation of a reporter construct with a different promoter (FasL) as well as an empty vector had no effect on the reporter activity (data not shown). These data proved a direct association between methylation and activity of the IRF-4 promoter."}

{"project":"test2","denotations":[{"id":"T9660","span":{"begin":27,"end":32},"obj":"Protein"},{"id":"T9661","span":{"begin":151,"end":156},"obj":"Protein"},{"id":"T9662","span":{"begin":214,"end":219},"obj":"Protein"},{"id":"T9663","span":{"begin":450,"end":455},"obj":"Protein"},{"id":"T9664","span":{"begin":479,"end":488},"obj":"Negative_regulation"},{"id":"T9665","span":{"begin":510,"end":515},"obj":"Protein"},{"id":"T9666","span":{"begin":516,"end":524},"obj":"Gene_expression"},{"id":"T9667","span":{"begin":620,"end":625},"obj":"Protein"},{"id":"T9668","span":{"begin":626,"end":634},"obj":"Gene_expression"},{"id":"T9669","span":{"begin":719,"end":724},"obj":"Protein"},{"id":"T9670","span":{"begin":725,"end":733},"obj":"Gene_expression"},{"id":"T9671","span":{"begin":844,"end":848},"obj":"Protein"},{"id":"T9672","span":{"begin":961,"end":972},"obj":"Binding"},{"id":"T9673","span":{"begin":1013,"end":1018},"obj":"Protein"}],"relations":[{"id":"R8032","pred":"themeOf","subj":"T9665","obj":"T9666"},{"id":"R8033","pred":"themeOf","subj":"T9667","obj":"T9668"},{"id":"R8034","pred":"themeOf","subj":"T9669","obj":"T9670"}],"attributes":[{"id":"M212","pred":"Negation","subj":"T9664","obj":"true"}],"text":"In vitro methylation of an IRF-4 promoter-reporter construct decreases its activity\nTo provide evidence for a direct effect of methylational status on IRF-4 promoter activity we performed reporter gene assays with IRF-4 promoter constructs before and after their in vitro methylation. A complete methylation of these constructs was checked via restriction assays with methylation-sensitive endonucleases (Figure 5A). Intriguingly, methylation of the IRF-4 promoter significantly decreased promoter activity in IRF-4-positive SD-1 cells by 85.0% (Figure 5B). The silencing effect of CpG methylation was not restricted to IRF-4-positive cells, since in vitro methylation led to a 92.9% abrogation of promoter activity in IRF-4-negative Jurkat cells (Figure 5C). In contrast, control methylation of a reporter construct with a different promoter (FasL) as well as an empty vector had no effect on the reporter activity (data not shown). These data proved a direct association between methylation and activity of the IRF-4 promoter."}