PMC:1283364 / 5655-5972 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/1283364","sourcedb":"PMC","sourceid":"1283364","source_url":"http://www.ncbi.nlm.nih.gov/pmc/1283364","text":"PrP.XhoI vector (also known as pPrPpE1/E2,3sal [15]) with the tetracycline-responsive promoter of pTetSplice (Life Technologies, Rockville, Maryland, United States), and then ligating mouse APP with a humanized Aβ domain (mo/huAPP695) cDNA into the new vector. We began by cloning the tetracycline-responsive promoter","tracks":[{"project":"craft-sa-dev","denotations":[{"id":"T1564","span":{"begin":9,"end":15},"obj":"NN"},{"id":"T1566","span":{"begin":16,"end":17},"obj":"-LRB-"},{"id":"T1567","span":{"begin":22,"end":27},"obj":"VBN"},{"id":"T1568","span":{"begin":17,"end":21},"obj":"RB"},{"id":"T1569","span":{"begin":28,"end":30},"obj":"IN"},{"id":"T1570","span":{"begin":31,"end":38},"obj":"NN"},{"id":"T1571","span":{"begin":42,"end":46},"obj":"NN"},{"id":"T1572","span":{"begin":38,"end":39},"obj":"HYPH"},{"id":"T1573","span":{"begin":39,"end":41},"obj":"NN"},{"id":"T1574","span":{"begin":41,"end":42},"obj":","},{"id":"T1575","span":{"begin":47,"end":48},"obj":"-LRB-"},{"id":"T1576","span":{"begin":48,"end":50},"obj":"CD"},{"id":"T1577","span":{"begin":50,"end":51},"obj":"-RRB-"},{"id":"T1578","span":{"begin":51,"end":52},"obj":"-RRB-"},{"id":"T1579","span":{"begin":53,"end":57},"obj":"IN"},{"id":"T1580","span":{"begin":58,"end":61},"obj":"DT"},{"id":"T1581","span":{"begin":86,"end":94},"obj":"NN"},{"id":"T1582","span":{"begin":62,"end":74},"obj":"NN"},{"id":"T1583","span":{"begin":75,"end":85},"obj":"JJ"},{"id":"T1584","span":{"begin":74,"end":75},"obj":"HYPH"},{"id":"T1585","span":{"begin":95,"end":97},"obj":"IN"},{"id":"T1586","span":{"begin":98,"end":108},"obj":"NN"},{"id":"T1587","span":{"begin":109,"end":110},"obj":"-LRB-"},{"id":"T1588","span":{"begin":115,"end":127},"obj":"NNP"},{"id":"T1589","span":{"begin":110,"end":114},"obj":"NNP"},{"id":"T1590","span":{"begin":127,"end":129},"obj":","},{"id":"T1591","span":{"begin":129,"end":138},"obj":"NNP"},{"id":"T1592","span":{"begin":138,"end":140},"obj":","},{"id":"T1593","span":{"begin":140,"end":148},"obj":"NNP"},{"id":"T1594","span":{"begin":148,"end":150},"obj":","},{"id":"T1595","span":{"begin":150,"end":156},"obj":"NNP"},{"id":"T1596","span":{"begin":157,"end":163},"obj":"NNP"},{"id":"T1597","span":{"begin":163,"end":164},"obj":"-RRB-"},{"id":"T1598","span":{"begin":164,"end":166},"obj":","},{"id":"T1599","span":{"begin":166,"end":169},"obj":"CC"},{"id":"T1600","span":{"begin":170,"end":174},"obj":"RB"},{"id":"T1601","span":{"begin":175,"end":183},"obj":"VBG"},{"id":"T1602","span":{"begin":184,"end":189},"obj":"NN"},{"id":"T1603","span":{"begin":190,"end":193},"obj":"NN"},{"id":"T1604","span":{"begin":194,"end":198},"obj":"IN"},{"id":"T1605","span":{"begin":199,"end":200},"obj":"DT"},{"id":"T1606","span":{"begin":214,"end":220},"obj":"NN"},{"id":"T1607","span":{"begin":201,"end":210},"obj":"JJ"},{"id":"T1608","span":{"begin":211,"end":213},"obj":"NN"},{"id":"T1609","span":{"begin":235,"end":239},"obj":"NN"},{"id":"T1610","span":{"begin":221,"end":222},"obj":"-LRB-"},{"id":"T1611","span":{"begin":222,"end":224},"obj":"NN"},{"id":"T1612","span":{"begin":225,"end":233},"obj":"NN"},{"id":"T1613","span":{"begin":224,"end":225},"obj":"HYPH"},{"id":"T1614","span":{"begin":233,"end":234},"obj":"-RRB-"},{"id":"T1615","span":{"begin":240,"end":244},"obj":"IN"},{"id":"T1616","span":{"begin":245,"end":248},"obj":"DT"},{"id":"T1617","span":{"begin":253,"end":259},"obj":"NN"},{"id":"T1618","span":{"begin":249,"end":252},"obj":"JJ"},{"id":"T1619","span":{"begin":259,"end":260},"obj":"."},{"id":"T1621","span":{"begin":261,"end":263},"obj":"PRP"},{"id":"T1622","span":{"begin":264,"end":269},"obj":"VBD"},{"id":"T1623","span":{"begin":270,"end":272},"obj":"IN"},{"id":"T1624","span":{"begin":273,"end":280},"obj":"VBG"},{"id":"T1625","span":{"begin":281,"end":284},"obj":"DT"},{"id":"T1626","span":{"begin":309,"end":317},"obj":"NN"},{"id":"T1627","span":{"begin":285,"end":297},"obj":"NN"},{"id":"T1628","span":{"begin":298,"end":308},"obj":"JJ"},{"id":"T1629","span":{"begin":297,"end":298},"obj":"HYPH"}],"relations":[{"id":"R943","pred":"punct","subj":"T1566","obj":"T1567"},{"id":"R945","pred":"advmod","subj":"T1568","obj":"T1567"},{"id":"R946","pred":"prep","subj":"T1569","obj":"T1567"},{"id":"R947","pred":"nmod","subj":"T1570","obj":"T1571"},{"id":"R948","pred":"pobj","subj":"T1571","obj":"T1569"},{"id":"R949","pred":"punct","subj":"T1572","obj":"T1571"},{"id":"R950","pred":"nmod","subj":"T1573","obj":"T1571"},{"id":"R951","pred":"punct","subj":"T1574","obj":"T1571"},{"id":"R952","pred":"punct","subj":"T1575","obj":"T1576"},{"id":"R953","pred":"parataxis","subj":"T1576","obj":"T1567"},{"id":"R954","pred":"punct","subj":"T1577","obj":"T1576"},{"id":"R955","pred":"punct","subj":"T1578","obj":"T1567"},{"id":"R957","pred":"det","subj":"T1580","obj":"T1581"},{"id":"R958","pred":"pobj","subj":"T1581","obj":"T1579"},{"id":"R959","pred":"npadvmod","subj":"T1582","obj":"T1583"},{"id":"R960","pred":"amod","subj":"T1583","obj":"T1581"},{"id":"R961","pred":"punct","subj":"T1584","obj":"T1583"},{"id":"R962","pred":"prep","subj":"T1585","obj":"T1581"},{"id":"R963","pred":"pobj","subj":"T1586","obj":"T1585"},{"id":"R964","pred":"punct","subj":"T1587","obj":"T1588"},{"id":"R965","pred":"parataxis","subj":"T1588","obj":"T1586"},{"id":"R966","pred":"compound","subj":"T1589","obj":"T1588"},{"id":"R967","pred":"punct","subj":"T1590","obj":"T1588"},{"id":"R968","pred":"npadvmod","subj":"T1591","obj":"T1588"},{"id":"R969","pred":"punct","subj":"T1592","obj":"T1588"},{"id":"R970","pred":"npadvmod","subj":"T1593","obj":"T1588"},{"id":"R971","pred":"punct","subj":"T1594","obj":"T1588"},{"id":"R972","pred":"compound","subj":"T1595","obj":"T1596"},{"id":"R973","pred":"npadvmod","subj":"T1596","obj":"T1588"},{"id":"R974","pred":"punct","subj":"T1597","obj":"T1588"},{"id":"R977","pred":"advmod","subj":"T1600","obj":"T1601"},{"id":"R979","pred":"compound","subj":"T1602","obj":"T1603"},{"id":"R980","pred":"dobj","subj":"T1603","obj":"T1601"},{"id":"R981","pred":"prep","subj":"T1604","obj":"T1601"},{"id":"R982","pred":"det","subj":"T1605","obj":"T1606"},{"id":"R983","pred":"nmod","subj":"T1606","obj":"T1609"},{"id":"R984","pred":"amod","subj":"T1607","obj":"T1606"},{"id":"R985","pred":"nmod","subj":"T1608","obj":"T1606"},{"id":"R986","pred":"pobj","subj":"T1609","obj":"T1604"},{"id":"R987","pred":"punct","subj":"T1610","obj":"T1606"},{"id":"R988","pred":"compound","subj":"T1611","obj":"T1612"},{"id":"R989","pred":"appos","subj":"T1612","obj":"T1606"},{"id":"R990","pred":"punct","subj":"T1613","obj":"T1612"},{"id":"R991","pred":"punct","subj":"T1614","obj":"T1609"},{"id":"R992","pred":"prep","subj":"T1615","obj":"T1601"},{"id":"R993","pred":"det","subj":"T1616","obj":"T1617"},{"id":"R994","pred":"pobj","subj":"T1617","obj":"T1615"},{"id":"R995","pred":"amod","subj":"T1618","obj":"T1617"},{"id":"R997","pred":"nsubj","subj":"T1621","obj":"T1622"},{"id":"R998","pred":"prep","subj":"T1623","obj":"T1622"},{"id":"R999","pred":"pcomp","subj":"T1624","obj":"T1623"},{"id":"R1000","pred":"det","subj":"T1625","obj":"T1626"},{"id":"R1001","pred":"dobj","subj":"T1626","obj":"T1624"},{"id":"R1002","pred":"npadvmod","subj":"T1627","obj":"T1628"},{"id":"R1003","pred":"amod","subj":"T1628","obj":"T1626"},{"id":"R1004","pred":"punct","subj":"T1629","obj":"T1628"}],"attributes":[{"subj":"T1564","pred":"source","obj":"craft-sa-dev"},{"subj":"T1566","pred":"source","obj":"craft-sa-dev"},{"subj":"T1567","pred":"source","obj":"craft-sa-dev"},{"subj":"T1568","pred":"source","obj":"craft-sa-dev"},{"subj":"T1569","pred":"source","obj":"craft-sa-dev"},{"subj":"T1570","pred":"source","obj":"craft-sa-dev"},{"subj":"T1571","pred":"source","obj":"craft-sa-dev"},{"subj":"T1572","pred":"source","obj":"craft-sa-dev"},{"subj":"T1573","pred":"source","obj":"craft-sa-dev"},{"subj":"T1574","pred":"source","obj":"craft-sa-dev"},{"subj":"T1575","pred":"source","obj":"craft-sa-dev"},{"subj":"T1576","pred":"source","obj":"craft-sa-dev"},{"subj":"T1577","pred":"source","obj":"craft-sa-dev"},{"subj":"T1578","pred":"source","obj":"craft-sa-dev"},{"subj":"T1579","pred":"source","obj":"craft-sa-dev"},{"subj":"T1580","pred":"source","obj":"craft-sa-dev"},{"subj":"T1581","pred":"source","obj":"craft-sa-dev"},{"subj":"T1582","pred":"source","obj":"craft-sa-dev"},{"subj":"T1583","pred":"source","obj":"craft-sa-dev"},{"subj":"T1584","pred":"source","obj":"craft-sa-dev"},{"subj":"T1585","pred":"source","obj":"craft-sa-dev"},{"subj":"T1586","pred":"source","obj":"craft-sa-dev"},{"subj":"T1587","pred":"source","obj":"craft-sa-dev"},{"subj":"T1588","pred":"source","obj":"craft-sa-dev"},{"subj":"T1589","pred":"source","obj":"craft-sa-dev"},{"subj":"T1590","pred":"source","obj":"craft-sa-dev"},{"subj":"T1591","pred":"source","obj":"craft-sa-dev"},{"subj":"T1592","pred":"source","obj":"craft-sa-dev"},{"subj":"T1593","pred":"source","obj":"craft-sa-dev"},{"subj":"T1594","pred":"source","obj":"craft-sa-dev"},{"subj":"T1595","pred":"source","obj":"craft-sa-dev"},{"subj":"T1596","pred":"source","obj":"craft-sa-dev"},{"subj":"T1597","pred":"source","obj":"craft-sa-dev"},{"subj":"T1598","pred":"source","obj":"craft-sa-dev"},{"subj":"T1599","pred":"source","obj":"craft-sa-dev"},{"subj":"T1600","pred":"source","obj":"craft-sa-dev"},{"subj":"T1601","pred":"source","obj":"craft-sa-dev"},{"subj":"T1602","pred":"source","obj":"craft-sa-dev"},{"subj":"T1603","pred":"source","obj":"craft-sa-dev"},{"subj":"T1604","pred":"source","obj":"craft-sa-dev"},{"subj":"T1605","pred":"source","obj":"craft-sa-dev"},{"subj":"T1606","pred":"source","obj":"craft-sa-dev"},{"subj":"T1607","pred":"source","obj":"craft-sa-dev"},{"subj":"T1608","pred":"source","obj":"craft-sa-dev"},{"subj":"T1609","pred":"source","obj":"craft-sa-dev"},{"subj":"T1610","pred":"source","obj":"craft-sa-dev"},{"subj":"T1611","pred":"source","obj":"craft-sa-dev"},{"subj":"T1612","pred":"source","obj":"craft-sa-dev"},{"subj":"T1613","pred":"source","obj":"craft-sa-dev"},{"subj":"T1614","pred":"source","obj":"craft-sa-dev"},{"subj":"T1615","pred":"source","obj":"craft-sa-dev"},{"subj":"T1616","pred":"source","obj":"craft-sa-dev"},{"subj":"T1617","pred":"source","obj":"craft-sa-dev"},{"subj":"T1618","pred":"source","obj":"craft-sa-dev"},{"subj":"T1619","pred":"source","obj":"craft-sa-dev"},{"subj":"T1621","pred":"source","obj":"craft-sa-dev"},{"subj":"T1622","pred":"source","obj":"craft-sa-dev"},{"subj":"T1623","pred":"source","obj":"craft-sa-dev"},{"subj":"T1624","pred":"source","obj":"craft-sa-dev"},{"subj":"T1625","pred":"source","obj":"craft-sa-dev"},{"subj":"T1626","pred":"source","obj":"craft-sa-dev"},{"subj":"T1627","pred":"source","obj":"craft-sa-dev"},{"subj":"T1628","pred":"source","obj":"craft-sa-dev"},{"subj":"T1629","pred":"source","obj":"craft-sa-dev"}]},{"project":"2_test","denotations":[{"id":"16279840-9117892-86242754","span":{"begin":48,"end":50},"obj":"9117892"},{"id":"T80824","span":{"begin":48,"end":50},"obj":"9117892"}],"attributes":[{"subj":"16279840-9117892-86242754","pred":"source","obj":"2_test"},{"subj":"T80824","pred":"source","obj":"2_test"}]},{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T1446","span":{"begin":9,"end":15},"obj":"SO_EXT:0000440"},{"id":"T1447","span":{"begin":62,"end":74},"obj":"CHEBI:27902"},{"id":"T1448","span":{"begin":86,"end":94},"obj":"SO_EXT:0000167"},{"id":"T1449","span":{"begin":175,"end":183},"obj":"GO:0006266"},{"id":"T1450","span":{"begin":184,"end":189},"obj":"NCBITaxon:10088"},{"id":"T1451","span":{"begin":190,"end":193},"obj":"PR_EXT:000004168"},{"id":"T1452","span":{"begin":211,"end":213},"obj":"CHEBI_PR_EXT:amyloid_beta"},{"id":"T1453","span":{"begin":214,"end":220},"obj":"SO_EXT:0000417"},{"id":"T1454","span":{"begin":222,"end":224},"obj":"NCBITaxon:10088"},{"id":"T1455","span":{"begin":235,"end":239},"obj":"SO_EXT:cDNA"},{"id":"T1456","span":{"begin":253,"end":259},"obj":"SO_EXT:0000440"},{"id":"T1457","span":{"begin":273,"end":280},"obj":"SO_EXT:sequence_cloning_process"},{"id":"T1458","span":{"begin":285,"end":297},"obj":"CHEBI:27902"},{"id":"T1459","span":{"begin":309,"end":317},"obj":"SO_EXT:0000167"}],"attributes":[{"subj":"T1446","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T1447","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T1448","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T1449","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T1450","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T1451","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T1452","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T1453","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T1454","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T1455","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T1456","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T1457","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T1458","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T1459","pred":"source","obj":"craft-ca-core-ex-dev"}]},{"project":"craft-ca-core-dev","denotations":[{"id":"T1368","span":{"begin":9,"end":15},"obj":"SO:0000440"},{"id":"T1369","span":{"begin":62,"end":74},"obj":"CHEBI:27902"},{"id":"T1370","span":{"begin":86,"end":94},"obj":"SO:0000167"},{"id":"T1371","span":{"begin":175,"end":183},"obj":"GO:0006266"},{"id":"T1372","span":{"begin":184,"end":189},"obj":"NCBITaxon:10088"},{"id":"T1373","span":{"begin":190,"end":193},"obj":"PR:000004168"},{"id":"T1374","span":{"begin":214,"end":220},"obj":"SO:0000417"},{"id":"T1375","span":{"begin":222,"end":224},"obj":"NCBITaxon:10088"},{"id":"T1376","span":{"begin":253,"end":259},"obj":"SO:0000440"},{"id":"T1377","span":{"begin":285,"end":297},"obj":"CHEBI:27902"},{"id":"T1378","span":{"begin":309,"end":317},"obj":"SO:0000167"}],"attributes":[{"subj":"T1368","pred":"source","obj":"craft-ca-core-dev"},{"subj":"T1369","pred":"source","obj":"craft-ca-core-dev"},{"subj":"T1370","pred":"source","obj":"craft-ca-core-dev"},{"subj":"T1371","pred":"source","obj":"craft-ca-core-dev"},{"subj":"T1372","pred":"source","obj":"craft-ca-core-dev"},{"subj":"T1373","pred":"source","obj":"craft-ca-core-dev"},{"subj":"T1374","pred":"source","obj":"craft-ca-core-dev"},{"subj":"T1375","pred":"source","obj":"craft-ca-core-dev"},{"subj":"T1376","pred":"source","obj":"craft-ca-core-dev"},{"subj":"T1377","pred":"source","obj":"craft-ca-core-dev"},{"subj":"T1378","pred":"source","obj":"craft-ca-core-dev"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"craft-sa-dev","color":"#b193ec","default":true},{"id":"2_test","color":"#93ec97"},{"id":"craft-ca-core-ex-dev","color":"#ec93a8"},{"id":"craft-ca-core-dev","color":"#93c2ec"}]}]}}