PMC:1255741 / 52725-54106 JSONTXT

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For exogeneous expression of GFPGluR-B, the mouse line TgOCN1 was generated: an AseI fragment of plasmid pnlacZ/GFPGluR-B was injected into the pronucleus of oocytes obtained from DBA1/C57Bl/6 F1 hybrids. Positive founders were backcrossed into C57Bl/6 for further analysis. Plasmid pnlacZ/GFPGluR-B was constructed from pnlacZ/GFPGluR-A [87] by replacing GluR-A cDNA with the rat cDNA for GluR-B. 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