Id |
Subject |
Object |
Predicate |
Lexical cue |
T1758 |
23-33 |
NN |
denotes |
Generation |
T1759 |
34-36 |
IN |
denotes |
of |
T1760 |
37-41 |
NN |
denotes |
Mtf1 |
T1761 |
63-67 |
NNS |
denotes |
mice |
T1762 |
42-53 |
JJ |
denotes |
conditional |
T1763 |
54-62 |
NN |
denotes |
knockout |
T1764 |
68-71 |
CC |
denotes |
and |
T1765 |
72-77 |
NN |
denotes |
liver |
T1766 |
78-86 |
JJ |
denotes |
specific |
T1767 |
77-78 |
HYPH |
denotes |
- |
T1768 |
87-95 |
NN |
denotes |
deletion |
T1769 |
95-200 |
sentence |
denotes |
Mtf1 conditional knockout mice were generated in collaboration with Dr Michael Leviten (San Carlos, CA). |
T1770 |
96-100 |
NN |
denotes |
Mtf1 |
T1771 |
122-126 |
NNS |
denotes |
mice |
T1772 |
101-112 |
JJ |
denotes |
conditional |
T1773 |
113-121 |
NN |
denotes |
knockout |
T1774 |
132-141 |
VBN |
denotes |
generated |
T1775 |
127-131 |
VBD |
denotes |
were |
T1776 |
142-144 |
IN |
denotes |
in |
T1777 |
145-158 |
NN |
denotes |
collaboration |
T1778 |
159-163 |
IN |
denotes |
with |
T1779 |
164-166 |
NNP |
denotes |
Dr |
T1780 |
175-182 |
NNP |
denotes |
Leviten |
T1781 |
167-174 |
NNP |
denotes |
Michael |
T1782 |
183-184 |
-LRB- |
denotes |
( |
T1783 |
188-194 |
NNP |
denotes |
Carlos |
T1784 |
184-187 |
NNP |
denotes |
San |
T1785 |
194-196 |
, |
denotes |
, |
T1786 |
196-198 |
NNP |
denotes |
CA |
T1787 |
198-199 |
-RRB- |
denotes |
) |
T1788 |
199-200 |
. |
denotes |
. |
T1789 |
200-349 |
sentence |
denotes |
Two genomic clones containing exons 3 to 6 of Mtf1 were used to construct a gene targeting vector for homologous recombination (Supplementary Data). |
T1790 |
201-204 |
CD |
denotes |
Two |
T1791 |
213-219 |
NNS |
denotes |
clones |
T1792 |
205-212 |
JJ |
denotes |
genomic |
T1793 |
257-261 |
VBN |
denotes |
used |
T1794 |
220-230 |
VBG |
denotes |
containing |
T1795 |
231-236 |
NNS |
denotes |
exons |
T1796 |
237-238 |
CD |
denotes |
3 |
T1797 |
239-241 |
IN |
denotes |
to |
T1798 |
242-243 |
CD |
denotes |
6 |
T1799 |
244-246 |
IN |
denotes |
of |
T1800 |
247-251 |
NN |
denotes |
Mtf1 |
T1801 |
252-256 |
VBD |
denotes |
were |
T1802 |
262-264 |
TO |
denotes |
to |
T1803 |
265-274 |
VB |
denotes |
construct |
T1804 |
275-276 |
DT |
denotes |
a |
T1805 |
292-298 |
NN |
denotes |
vector |
T1806 |
277-281 |
NN |
denotes |
gene |
T1807 |
282-291 |
NN |
denotes |
targeting |
T1808 |
299-302 |
IN |
denotes |
for |
T1809 |
303-313 |
JJ |
denotes |
homologous |
T1810 |
314-327 |
NN |
denotes |
recombination |
T1811 |
328-329 |
-LRB- |
denotes |
( |
T1812 |
343-347 |
NNS |
denotes |
Data |
T1813 |
329-342 |
JJ |
denotes |
Supplementary |
T1814 |
347-348 |
-RRB- |
denotes |
) |
T1815 |
348-349 |
. |
denotes |
. |
T1816 |
349-532 |
sentence |
denotes |
A neomycin resistance cassette (PGK-neo) flanked by two loxP sites was cloned into the SacI site 5′ of exon 3 of Mtf1, the third loxP site was cloned into the ScaI site 3′ of exon 4. |
T1817 |
350-351 |
DT |
denotes |
A |
T1818 |
372-380 |
NN |
denotes |
cassette |
T1819 |
352-360 |
NN |
denotes |
neomycin |
T1820 |
361-371 |
NN |
denotes |
resistance |
T1821 |
421-427 |
VBN |
denotes |
cloned |
T1822 |
381-382 |
-LRB- |
denotes |
( |
T1823 |
382-385 |
NN |
denotes |
PGK |
T1824 |
386-389 |
NN |
denotes |
neo |
T1825 |
385-386 |
HYPH |
denotes |
- |
T1826 |
389-390 |
-RRB- |
denotes |
) |
T1827 |
391-398 |
VBN |
denotes |
flanked |
T1828 |
399-401 |
IN |
denotes |
by |
T1829 |
402-405 |
CD |
denotes |
two |
T1830 |
411-416 |
NNS |
denotes |
sites |
T1831 |
406-410 |
NN |
denotes |
loxP |
T1832 |
417-420 |
VBD |
denotes |
was |
T1833 |
493-499 |
VBN |
denotes |
cloned |
T1834 |
428-432 |
IN |
denotes |
into |
T1835 |
433-436 |
DT |
denotes |
the |
T1836 |
442-446 |
NN |
denotes |
site |
T1837 |
437-441 |
NN |
denotes |
SacI |
T1838 |
447-448 |
CD |
denotes |
5 |
T1839 |
448-449 |
SYM |
denotes |
′ |
T1840 |
450-452 |
IN |
denotes |
of |
T1841 |
453-457 |
NN |
denotes |
exon |
T1842 |
458-459 |
CD |
denotes |
3 |
T1843 |
460-462 |
IN |
denotes |
of |
T1844 |
463-467 |
NN |
denotes |
Mtf1 |
T1845 |
467-469 |
, |
denotes |
, |
T1846 |
469-472 |
DT |
denotes |
the |
T1847 |
484-488 |
NN |
denotes |
site |
T1848 |
473-478 |
JJ |
denotes |
third |
T1849 |
479-483 |
NN |
denotes |
loxP |
T1850 |
489-492 |
VBD |
denotes |
was |
T1851 |
500-504 |
IN |
denotes |
into |
T1852 |
505-508 |
DT |
denotes |
the |
T1853 |
514-518 |
NN |
denotes |
site |
T1854 |
509-513 |
NN |
denotes |
ScaI |
T1855 |
519-520 |
CD |
denotes |
3 |
T1856 |
520-521 |
SYM |
denotes |
′ |
T1857 |
522-524 |
IN |
denotes |
of |
T1858 |
525-529 |
NN |
denotes |
exon |
T1859 |
530-531 |
CD |
denotes |
4 |
T1860 |
531-532 |
. |
denotes |
. |
T1861 |
532-609 |
sentence |
denotes |
A thymidine kinase (TK) cassette was inserted in the HpaI site 3′ of exon 6. |
T1862 |
533-534 |
DT |
denotes |
A |
T1863 |
557-565 |
NN |
denotes |
cassette |
T1864 |
535-544 |
NN |
denotes |
thymidine |
T1865 |
545-551 |
NN |
denotes |
kinase |
T1866 |
552-553 |
-LRB- |
denotes |
( |
T1867 |
553-555 |
NN |
denotes |
TK |
T1868 |
555-556 |
-RRB- |
denotes |
) |
T1869 |
570-578 |
VBN |
denotes |
inserted |
T1870 |
566-569 |
VBD |
denotes |
was |
T1871 |
579-581 |
IN |
denotes |
in |
T1872 |
582-585 |
DT |
denotes |
the |
T1873 |
591-595 |
NN |
denotes |
site |
T1874 |
586-590 |
NN |
denotes |
HpaI |
T1875 |
596-597 |
CD |
denotes |
3 |
T1876 |
597-598 |
SYM |
denotes |
′ |
T1877 |
599-601 |
IN |
denotes |
of |
T1878 |
602-606 |
NN |
denotes |
exon |
T1879 |
607-608 |
CD |
denotes |
6 |
T1880 |
608-609 |
. |
denotes |
. |
T1881 |
609-802 |
sentence |
denotes |
129 ES cells were electroporated with the linearized targeting vector, selected in the presence of G418 and FIAU, and screened for correct integration events by PCR and Southern blot analysis. |
T1882 |
610-613 |
CD |
denotes |
129 |
T1883 |
617-622 |
NNS |
denotes |
cells |
T1884 |
614-616 |
NN |
denotes |
ES |
T1885 |
628-642 |
VBN |
denotes |
electroporated |
T1886 |
623-627 |
VBD |
denotes |
were |
T1887 |
643-647 |
IN |
denotes |
with |
T1888 |
648-651 |
DT |
denotes |
the |
T1889 |
673-679 |
NN |
denotes |
vector |
T1890 |
652-662 |
VBN |
denotes |
linearized |
T1891 |
663-672 |
NN |
denotes |
targeting |
T1892 |
679-681 |
, |
denotes |
, |
T1893 |
681-689 |
VBN |
denotes |
selected |
T1894 |
690-692 |
IN |
denotes |
in |
T1895 |
693-696 |
DT |
denotes |
the |
T1896 |
697-705 |
NN |
denotes |
presence |
T1897 |
706-708 |
IN |
denotes |
of |
T1898 |
709-713 |
NN |
denotes |
G418 |
T1899 |
714-717 |
CC |
denotes |
and |
T1900 |
718-722 |
NN |
denotes |
FIAU |
T1901 |
722-724 |
, |
denotes |
, |
T1902 |
724-727 |
CC |
denotes |
and |
T1903 |
728-736 |
VBN |
denotes |
screened |
T1904 |
737-740 |
IN |
denotes |
for |
T1905 |
741-748 |
JJ |
denotes |
correct |
T1906 |
761-767 |
NNS |
denotes |
events |
T1907 |
749-760 |
NN |
denotes |
integration |
T1908 |
768-770 |
IN |
denotes |
by |
T1909 |
771-774 |
NN |
denotes |
PCR |
T1910 |
793-801 |
NN |
denotes |
analysis |
T1911 |
775-778 |
CC |
denotes |
and |
T1912 |
779-787 |
NNP |
denotes |
Southern |
T1913 |
788-792 |
NN |
denotes |
blot |
T1914 |
801-802 |
. |
denotes |
. |
T1915 |
802-1025 |
sentence |
denotes |
Transient expression of Cre recombinase led to removal of the PGK-neo cassette, and mice carrying the modified Mtf1loxP allele were generated by injection of positive clones into C57Bl/6 blastocysts and subsequent crosses. |
T1916 |
803-812 |
JJ |
denotes |
Transient |
T1917 |
813-823 |
NN |
denotes |
expression |
T1918 |
843-846 |
VBD |
denotes |
led |
T1919 |
824-826 |
IN |
denotes |
of |
T1920 |
827-830 |
NN |
denotes |
Cre |
T1921 |
831-842 |
NN |
denotes |
recombinase |
T1922 |
847-849 |
IN |
denotes |
to |
T1923 |
850-857 |
NN |
denotes |
removal |
T1924 |
858-860 |
IN |
denotes |
of |
T1925 |
861-864 |
DT |
denotes |
the |
T1926 |
873-881 |
NN |
denotes |
cassette |
T1927 |
865-868 |
NN |
denotes |
PGK |
T1928 |
869-872 |
NN |
denotes |
neo |
T1929 |
868-869 |
HYPH |
denotes |
- |
T1930 |
881-883 |
, |
denotes |
, |
T1931 |
883-886 |
CC |
denotes |
and |
T1932 |
887-891 |
NNS |
denotes |
mice |
T1933 |
935-944 |
VBN |
denotes |
generated |
T1934 |
892-900 |
VBG |
denotes |
carrying |
T1935 |
901-904 |
DT |
denotes |
the |
T1936 |
923-929 |
NN |
denotes |
allele |
T1937 |
905-913 |
VBN |
denotes |
modified |
T1938 |
914-922 |
NN |
denotes |
Mtf1loxP |
T1939 |
930-934 |
VBD |
denotes |
were |
T1940 |
945-947 |
IN |
denotes |
by |
T1941 |
948-957 |
NN |
denotes |
injection |
T1942 |
958-960 |
IN |
denotes |
of |
T1943 |
961-969 |
JJ |
denotes |
positive |
T1944 |
970-976 |
NNS |
denotes |
clones |
T1945 |
977-981 |
IN |
denotes |
into |
T1946 |
982-987 |
NN |
denotes |
C57Bl |
T1947 |
990-1001 |
NNS |
denotes |
blastocysts |
T1948 |
987-988 |
HYPH |
denotes |
/ |
T1949 |
988-989 |
CD |
denotes |
6 |
T1950 |
1002-1005 |
CC |
denotes |
and |
T1951 |
1006-1016 |
JJ |
denotes |
subsequent |
T1952 |
1017-1024 |
NNS |
denotes |
crosses |
T1953 |
1024-1025 |
. |
denotes |
. |
T1955 |
1026-1036 |
JJ |
denotes |
Homozygous |
T1956 |
1058-1065 |
NNS |
denotes |
animals |
T1957 |
1037-1048 |
JJ |
denotes |
conditional |
T1958 |
1049-1057 |
NN |
denotes |
knockout |
T1959 |
1087-1094 |
VBN |
denotes |
crossed |
T1960 |
1066-1067 |
-LRB- |
denotes |
( |
T1961 |
1067-1075 |
NN |
denotes |
Mtf1loxP |
T1962 |
1076-1080 |
NN |
denotes |
loxP |
T1963 |
1075-1076 |
HYPH |
denotes |
/ |
T1964 |
1080-1081 |
-RRB- |
denotes |
) |
T1965 |
1082-1086 |
VBD |
denotes |
were |
T1966 |
1095-1099 |
IN |
denotes |
with |
T1967 |
1100-1103 |
DT |
denotes |
the |
T1968 |
1131-1135 |
NN |
denotes |
line |
T2099 |
1233-1234 |
. |
denotes |
. |
T2100 |
1234-1488 |
sentence |
denotes |
The mice were genotyped by PCR using the following primers (Microsynth): Cre recombinase: 5′-CTATCCAGCAACATTTGGGCCAGC-3′; 5′-CCAGGTTACGGATATAGTTCATGAC-3′, Mtf1loxP or wild-type allele: 5′-CACACCCAGTTTGTGTATGTCTTC-3′; 5′-CAGTCACAAGCAAATTACCAAACACTGCC-3′. |
T2101 |
1235-1238 |
DT |
denotes |
The |
T2102 |
1239-1243 |
NNS |
denotes |
mice |
T2103 |
1249-1258 |
VBN |
denotes |
genotyped |
T2104 |
1244-1248 |
VBD |
denotes |
were |
T2105 |
1259-1261 |
IN |
denotes |
by |
T2106 |
1262-1265 |
NN |
denotes |
PCR |
T2107 |
1266-1271 |
VBG |
denotes |
using |
T2108 |
1272-1275 |
DT |
denotes |
the |
T2109 |
1286-1293 |
NNS |
denotes |
primers |
T2110 |
1276-1285 |
VBG |
denotes |
following |
T2111 |
1294-1295 |
-LRB- |
denotes |
( |
T2112 |
1295-1305 |
NNP |
denotes |
Microsynth |
T2113 |
1305-1306 |
-RRB- |
denotes |
) |
T2114 |
1306-1308 |
: |
denotes |
: |
T2115 |
1308-1311 |
NN |
denotes |
Cre |
T2116 |
1312-1323 |
NN |
denotes |
recombinase |
T2117 |
1360-1385 |
NN |
denotes |
CCAGGTTACGGATATAGTTCATGAC |
T2118 |
1323-1325 |
: |
denotes |
: |
T2119 |
1325-1326 |
CD |
denotes |
5 |
T2120 |
1328-1352 |
NN |
denotes |
CTATCCAGCAACATTTGGGCCAGC |
T2121 |
1326-1327 |
SYM |
denotes |
′ |
T2122 |
1327-1328 |
HYPH |
denotes |
- |
T2123 |
1352-1353 |
HYPH |
denotes |
- |
T2124 |
1353-1354 |
CD |
denotes |
3 |
T2125 |
1354-1355 |
SYM |
denotes |
′ |
T2126 |
1355-1356 |
: |
denotes |
; |
T2127 |
1357-1358 |
CD |
denotes |
5 |
T2128 |
1358-1359 |
SYM |
denotes |
′ |
T2129 |
1359-1360 |
HYPH |
denotes |
- |
T2130 |
1455-1484 |
NN |
denotes |
CAGTCACAAGCAAATTACCAAACACTGCC |
T2131 |
1385-1386 |
HYPH |
denotes |
- |
T2132 |
1386-1387 |
CD |
denotes |
3 |
T2133 |
1387-1388 |
SYM |
denotes |
′ |
T2134 |
1388-1390 |
, |
denotes |
, |
T2135 |
1390-1398 |
NN |
denotes |
Mtf1loxP |
T2136 |
1399-1401 |
CC |
denotes |
or |
T2137 |
1402-1406 |
JJ |
denotes |
wild |
T2138 |
1407-1411 |
NN |
denotes |
type |
T2139 |
1406-1407 |
HYPH |
denotes |
- |
T2140 |
1412-1418 |
NN |
denotes |
allele |
T2141 |
1418-1420 |
: |
denotes |
: |
T2142 |
1420-1421 |
CD |
denotes |
5 |
T2143 |
1423-1447 |
NN |
denotes |
CACACCCAGTTTGTGTATGTCTTC |
T2144 |
1421-1422 |
SYM |
denotes |
′ |
T2145 |
1422-1423 |
HYPH |
denotes |
- |
T2146 |
1447-1448 |
HYPH |
denotes |
- |
T2147 |
1448-1449 |
CD |
denotes |
3 |
T2148 |
1449-1450 |
SYM |
denotes |
′ |
T2149 |
1450-1451 |
: |
denotes |
; |
T2150 |
1452-1453 |
CD |
denotes |
5 |
T2151 |
1453-1454 |
SYM |
denotes |
′ |
T2152 |
1454-1455 |
HYPH |
denotes |
- |
T2153 |
1484-1485 |
HYPH |
denotes |
- |
T2154 |
1485-1486 |
CD |
denotes |
3 |
T2155 |
1486-1487 |
SYM |
denotes |
′ |
T2156 |
1487-1488 |
. |
denotes |
. |
T2531 |
2321-2331 |
NN |
denotes |
Microarray |
T2532 |
2332-2340 |
NN |
denotes |
analysis |
T2533 |
2341-2344 |
CC |
denotes |
and |
T2534 |
2345-2349 |
NNS |
denotes |
data |
T2535 |
2350-2360 |
NN |
denotes |
processing |
T2536 |
2360-2586 |
sentence |
denotes |
Total RNA was isolated from liver tissue of pI–pC-induced, mock- or cadmium-treated Mtf1Mx-cre and Mtf1loxP mice (n = 3 per genotype and respective treatment; all male) essentially as described by Chomczynski and Sacchi (27). |
T2537 |
2361-2366 |
JJ |
denotes |
Total |
T2538 |
2367-2370 |
NN |
denotes |
RNA |
T2539 |
2375-2383 |
VBN |
denotes |
isolated |
T2540 |
2371-2374 |
VBD |
denotes |
was |
T2541 |
2384-2388 |
IN |
denotes |
from |
T2542 |
2389-2394 |
NN |
denotes |
liver |
T2543 |
2395-2401 |
NN |
denotes |
tissue |
T2544 |
2402-2404 |
IN |
denotes |
of |
T2545 |
2405-2407 |
NN |
denotes |
pI |
T2546 |
2408-2410 |
NN |
denotes |
pC |
T2547 |
2407-2408 |
HYPH |
denotes |
– |
T2548 |
2411-2418 |
VBN |
denotes |
induced |
T2549 |
2410-2411 |
HYPH |
denotes |
- |
T2550 |
2469-2473 |
NNS |
denotes |
mice |
T2551 |
2418-2420 |
, |
denotes |
, |
T2552 |
2420-2424 |
JJ |
denotes |
mock |
T2553 |
2437-2444 |
VBN |
denotes |
treated |
T2554 |
2424-2425 |
HYPH |
denotes |
- |
T2555 |
2426-2428 |
CC |
denotes |
or |
T2556 |
2429-2436 |
NN |
denotes |
cadmium |
T2557 |
2436-2437 |
HYPH |
denotes |
- |
T2558 |
2445-2451 |
NN |
denotes |
Mtf1Mx |
T2559 |
2452-2455 |
NN |
denotes |
cre |
T2560 |
2451-2452 |
HYPH |
denotes |
- |
T2561 |
2456-2459 |
CC |
denotes |
and |
T2562 |
2460-2468 |
NN |
denotes |
Mtf1loxP |
T2563 |
2474-2475 |
-LRB- |
denotes |
( |
T2564 |
2524-2528 |
JJ |
denotes |
male |
T2565 |
2475-2476 |
NN |
denotes |
n |
T2566 |
2479-2480 |
CD |
denotes |
3 |
T2567 |
2477-2478 |
SYM |
denotes |
= |
T2568 |
2481-2484 |
IN |
denotes |
per |
T2569 |
2485-2493 |
NN |
denotes |
genotype |
T2570 |
2494-2497 |
CC |
denotes |
and |
T2571 |
2498-2508 |
JJ |
denotes |
respective |
T2572 |
2509-2518 |
NN |
denotes |
treatment |
T2573 |
2518-2519 |
: |
denotes |
; |
T2574 |
2520-2523 |
DT |
denotes |
all |
T2575 |
2528-2529 |
-RRB- |
denotes |
) |
T2576 |
2530-2541 |
RB |
denotes |
essentially |
T2577 |
2545-2554 |
VBN |
denotes |
described |
T2578 |
2542-2544 |
IN |
denotes |
as |
T2579 |
2555-2557 |
IN |
denotes |
by |
T2580 |
2558-2569 |
NNP |
denotes |
Chomczynski |
T2581 |
2570-2573 |
CC |
denotes |
and |
T2582 |
2574-2580 |
NNP |
denotes |
Sacchi |
T2583 |
2581-2582 |
-LRB- |
denotes |
( |
T2584 |
2582-2584 |
CD |
denotes |
27 |
T2585 |
2584-2585 |
-RRB- |
denotes |
) |
T2586 |
2585-2586 |
. |
denotes |
. |
T2587 |
2586-2811 |
sentence |
denotes |
Gene expression analysis was performed in the Functional Genomics Center Zurich using GeneChip® Mouse Genome 430 2.0 Arrays (Affymetrix) according to the manufacturer's instructions and the following reagents and conditions. |
T2588 |
2587-2591 |
NN |
denotes |
Gene |
T2589 |
2592-2602 |
NN |
denotes |
expression |
T2590 |
2603-2611 |
NN |
denotes |
analysis |
T2591 |
2616-2625 |
VBN |
denotes |
performed |
T2592 |
2612-2615 |
VBD |
denotes |
was |
T2593 |
2626-2628 |
IN |
denotes |
in |
T2594 |
2629-2632 |
DT |
denotes |
the |
T2595 |
2653-2659 |
NNP |
denotes |
Center |
T2596 |
2633-2643 |
NNP |
denotes |
Functional |
T2597 |
2644-2652 |
NNP |
denotes |
Genomics |
T2598 |
2660-2666 |
NNP |
denotes |
Zurich |
T2599 |
2667-2672 |
VBG |
denotes |
using |
T2600 |
2673-2682 |
NNP |
denotes |
GeneChip® |
T2601 |
2704-2710 |
NNS |
denotes |
Arrays |
T2602 |
2683-2688 |
NNP |
denotes |
Mouse |
T2603 |
2689-2695 |
NNP |
denotes |
Genome |
T2604 |
2696-2699 |
CD |
denotes |
430 |
T2605 |
2700-2703 |
CD |
denotes |
2.0 |
T2606 |
2711-2712 |
-LRB- |
denotes |
( |
T2607 |
2712-2722 |
NNP |
denotes |
Affymetrix |
T2608 |
2722-2723 |
-RRB- |
denotes |
) |
T2609 |
2724-2733 |
VBG |
denotes |
according |
T2610 |
2734-2736 |
IN |
denotes |
to |
T2611 |
2737-2740 |
DT |
denotes |
the |
T2612 |
2741-2753 |
NN |
denotes |
manufacturer |
T2613 |
2756-2768 |
NNS |
denotes |
instructions |
T2614 |
2753-2755 |
POS |
denotes |
's |
T2615 |
2769-2772 |
CC |
denotes |
and |
T2616 |
2773-2776 |
DT |
denotes |
the |
T2617 |
2787-2795 |
NNS |
denotes |
reagents |
T2618 |
2777-2786 |
VBG |
denotes |
following |
T2619 |
2796-2799 |
CC |
denotes |
and |
T2620 |
2800-2810 |
NNS |
denotes |
conditions |
T2621 |
2810-2811 |
. |
denotes |
. |
T2622 |
2811-2922 |
sentence |
denotes |
cDNA was synthesized with SuperScript™ Double-Stranded cDNA Synthesis Kit (Invitrogen), using 15 µg total RNA. |
T2623 |
2812-2816 |
NN |
denotes |
cDNA |
T2624 |
2821-2832 |
VBN |
denotes |
synthesized |
T2625 |
2817-2820 |
VBD |
denotes |
was |
T2626 |
2833-2837 |
IN |
denotes |
with |
T2627 |
2838-2849 |
NNP |
denotes |
SuperScript |
T2628 |
2882-2885 |
NN |
denotes |
Kit |
T2629 |
2849-2850 |
SYM |
denotes |
™ |
T2630 |
2851-2857 |
JJ |
denotes |
Double |
T2631 |
2858-2866 |
VBN |
denotes |
Stranded |
T2632 |
2857-2858 |
HYPH |
denotes |
- |
T2633 |
2867-2871 |
NN |
denotes |
cDNA |
T2634 |
2872-2881 |
NN |
denotes |
Synthesis |
T2635 |
2886-2887 |
-LRB- |
denotes |
( |
T2636 |
2887-2897 |
NNP |
denotes |
Invitrogen |
T2637 |
2897-2898 |
-RRB- |
denotes |
) |
T2638 |
2898-2900 |
, |
denotes |
, |
T2639 |
2900-2905 |
VBG |
denotes |
using |
T2640 |
2906-2908 |
CD |
denotes |
15 |
T2641 |
2909-2911 |
NN |
denotes |
µg |
T2642 |
2918-2921 |
NN |
denotes |
RNA |
T2643 |
2912-2917 |
JJ |
denotes |
total |
T2644 |
2921-2922 |
. |
denotes |
. |
T2645 |
2922-3051 |
sentence |
denotes |
In vitro transcription was performed with BioArray™ High Yield™ RNA Transcript Labeling Kit (Enzo) and 3.5 to 6 µg of each cDNA. |
T2646 |
2923-2925 |
FW |
denotes |
In |
T2647 |
2926-2931 |
FW |
denotes |
vitro |
T2648 |
2932-2945 |
NN |
denotes |
transcription |
T2649 |
2950-2959 |
VBN |
denotes |
performed |
T2650 |
2946-2949 |
VBD |
denotes |
was |
T2651 |
2960-2964 |
IN |
denotes |
with |
T2652 |
2965-2973 |
NNP |
denotes |
BioArray |
T2653 |
3011-3014 |
NN |
denotes |
Kit |
T2654 |
2973-2974 |
SYM |
denotes |
™ |
T2655 |
2975-2979 |
NNP |
denotes |
High |
T2656 |
2980-2985 |
NNP |
denotes |
Yield |
T2657 |
2985-2986 |
SYM |
denotes |
™ |
T2658 |
2987-2990 |
NN |
denotes |
RNA |
T2659 |
2991-3001 |
NN |
denotes |
Transcript |
T2660 |
3002-3010 |
NN |
denotes |
Labeling |
T2661 |
3015-3016 |
-LRB- |
denotes |
( |
T2662 |
3016-3020 |
NNP |
denotes |
Enzo |
T2663 |
3020-3021 |
-RRB- |
denotes |
) |
T2664 |
3022-3025 |
CC |
denotes |
and |
T2665 |
3026-3029 |
CD |
denotes |
3.5 |
T2666 |
3033-3034 |
CD |
denotes |
6 |
T2667 |
3030-3032 |
IN |
denotes |
to |
T2668 |
3035-3037 |
NN |
denotes |
µg |
T2669 |
3038-3040 |
IN |
denotes |
of |
T2670 |
3041-3045 |
DT |
denotes |
each |
T2671 |
3046-3050 |
NN |
denotes |
cDNA |
T2672 |
3050-3051 |
. |
denotes |
. |
T2673 |
3051-3152 |
sentence |
denotes |
Clean-up of both cDNA and cRNA samples was done using GeneChip® Sample Clean-up Module (Affymetrix). |
T2674 |
3052-3057 |
NN |
denotes |
Clean |
T2675 |
3058-3060 |
NN |
denotes |
up |
T2676 |
3057-3058 |
HYPH |
denotes |
- |
T2677 |
3095-3099 |
VBN |
denotes |
done |
T2678 |
3061-3063 |
IN |
denotes |
of |
T2679 |
3064-3068 |
CC |
denotes |
both |
T2680 |
3069-3073 |
NN |
denotes |
cDNA |
T2681 |
3083-3090 |
NNS |
denotes |
samples |
T2682 |
3074-3077 |
CC |
denotes |
and |
T2683 |
3078-3082 |
NN |
denotes |
cRNA |
T2684 |
3091-3094 |
VBD |
denotes |
was |
T2685 |
3100-3105 |
VBG |
denotes |
using |
T2686 |
3106-3115 |
NNP |
denotes |
GeneChip® |
T2687 |
3132-3138 |
NNP |
denotes |
Module |
T2688 |
3116-3122 |
NNP |
denotes |
Sample |
T2689 |
3123-3128 |
NNP |
denotes |
Clean |
T2690 |
3129-3131 |
NNP |
denotes |
up |
T2691 |
3128-3129 |
HYPH |
denotes |
- |
T2692 |
3139-3140 |
-LRB- |
denotes |
( |
T2693 |
3140-3150 |
NNP |
denotes |
Affymetrix |
T2694 |
3150-3151 |
-RRB- |
denotes |
) |
T2695 |
3151-3152 |
. |
denotes |
. |
T2696 |
3152-3270 |
sentence |
denotes |
For the automated washing and staining in the Affymetrix fluidics station 450, the protocol EukGE-Ws2v4_450 was used. |
T2697 |
3153-3156 |
IN |
denotes |
For |
T2698 |
3265-3269 |
VBN |
denotes |
used |
T2699 |
3157-3160 |
DT |
denotes |
the |
T2700 |
3171-3178 |
NN |
denotes |
washing |
T2701 |
3161-3170 |
VBN |
denotes |
automated |
T2702 |
3179-3182 |
CC |
denotes |
and |
T2703 |
3183-3191 |
NN |
denotes |
staining |
T2704 |
3192-3194 |
IN |
denotes |
in |
T2705 |
3195-3198 |
DT |
denotes |
the |
T2706 |
3219-3226 |
NN |
denotes |
station |
T2707 |
3199-3209 |
NNP |
denotes |
Affymetrix |
T2708 |
3210-3218 |
NN |
denotes |
fluidics |
T2709 |
3227-3230 |
CD |
denotes |
450 |
T2710 |
3230-3232 |
, |
denotes |
, |
T2711 |
3232-3235 |
DT |
denotes |
the |
T2712 |
3251-3260 |
NN |
denotes |
Ws2v4_450 |
T2713 |
3236-3244 |
NN |
denotes |
protocol |
T2714 |
3245-3250 |
NN |
denotes |
EukGE |
T2715 |
3250-3251 |
HYPH |
denotes |
- |
T2716 |
3261-3264 |
VBD |
denotes |
was |
T2717 |
3269-3270 |
. |
denotes |
. |
T2718 |
3270-3337 |
sentence |
denotes |
The probe arrays were scanned with the Affymetrix GS 3000 scanner. |
T2719 |
3271-3274 |
DT |
denotes |
The |
T2720 |
3281-3287 |
NNS |
denotes |
arrays |
T2721 |
3275-3280 |
NN |
denotes |
probe |
T2722 |
3293-3300 |
VBN |
denotes |
scanned |
T2723 |
3288-3292 |
VBD |
denotes |
were |
T2724 |
3301-3305 |
IN |
denotes |
with |
T2725 |
3306-3309 |
DT |
denotes |
the |
T2726 |
3329-3336 |
NN |
denotes |
scanner |
T2727 |
3310-3320 |
NNP |
denotes |
Affymetrix |
T2728 |
3321-3323 |
NNP |
denotes |
GS |
T2729 |
3324-3328 |
CD |
denotes |
3000 |
T2730 |
3336-3337 |
. |
denotes |
. |
T2731 |
3337-3409 |
sentence |
denotes |
Raw data are available at ArrayExpress (, accession number E-MEXP-438). |
T2732 |
3338-3341 |
JJ |
denotes |
Raw |
T2733 |
3342-3346 |
NNS |
denotes |
data |
T2734 |
3347-3350 |
VBP |
denotes |
are |
T2735 |
3351-3360 |
JJ |
denotes |
available |
T2736 |
3361-3363 |
IN |
denotes |
at |
T2737 |
3364-3376 |
NNP |
denotes |
ArrayExpress |
T2738 |
3377-3378 |
-LRB- |
denotes |
( |
T2739 |
3399-3403 |
NN |
denotes |
MEXP |
T2740 |
3378-3380 |
, |
denotes |
, |
T2741 |
3380-3389 |
NN |
denotes |
accession |
T2742 |
3390-3396 |
NN |
denotes |
number |
T2743 |
3397-3398 |
NN |
denotes |
E |
T2744 |
3398-3399 |
HYPH |
denotes |
- |
T2745 |
3403-3404 |
HYPH |
denotes |
- |
T2746 |
3404-3407 |
CD |
denotes |
438 |
T2747 |
3407-3408 |
-RRB- |
denotes |
) |
T2748 |
3408-3409 |
. |
denotes |
. |
T2749 |
3409-3526 |
sentence |
denotes |
Data analysis was performed with GeneSpring 6.1 software (Silicon Genetics), applying a significance level P ≤ 0.05. |
T2750 |
3410-3414 |
NNS |
denotes |
Data |
T2751 |
3415-3423 |
NN |
denotes |
analysis |
T2752 |
3428-3437 |
VBN |
denotes |
performed |
T2753 |
3424-3427 |
VBD |
denotes |
was |
T2754 |
3438-3442 |
IN |
denotes |
with |
T2755 |
3443-3453 |
NNP |
denotes |
GeneSpring |
T2756 |
3458-3466 |
NN |
denotes |
software |
T2757 |
3454-3457 |
CD |
denotes |
6.1 |
T2758 |
3467-3468 |
-LRB- |
denotes |
( |
T2759 |
3476-3484 |
NNP |
denotes |
Genetics |
T2760 |
3468-3475 |
NNP |
denotes |
Silicon |
T2761 |
3484-3485 |
-RRB- |
denotes |
) |
T2762 |
3485-3487 |
, |
denotes |
, |
T2763 |
3487-3495 |
VBG |
denotes |
applying |
T2764 |
3496-3497 |
DT |
denotes |
a |
T2765 |
3511-3516 |
NN |
denotes |
level |
T2766 |
3498-3510 |
NN |
denotes |
significance |
T2767 |
3517-3518 |
NN |
denotes |
P |
T2768 |
3521-3525 |
CD |
denotes |
0.05 |
T2769 |
3519-3520 |
SYM |
denotes |
≤ |
T2770 |
3525-3526 |
. |
denotes |
. |
T2771 |
3526-3643 |
sentence |
denotes |
Furthermore, multiple testing correction was used in addition to obtain cadmium-responsive, MTF-1-independent genes. |
T2772 |
3527-3538 |
RB |
denotes |
Furthermore |
T2773 |
3572-3576 |
VBN |
denotes |
used |
T2774 |
3538-3540 |
, |
denotes |
, |
T2775 |
3540-3548 |
JJ |
denotes |
multiple |
T2776 |
3557-3567 |
NN |
denotes |
correction |
T2777 |
3549-3556 |
NN |
denotes |
testing |
T2778 |
3568-3571 |
VBD |
denotes |
was |
T2779 |
3577-3579 |
IN |
denotes |
in |
T2780 |
3580-3588 |
NN |
denotes |
addition |
T2781 |
3589-3591 |
TO |
denotes |
to |
T2782 |
3592-3598 |
VB |
denotes |
obtain |
T2783 |
3599-3606 |
NN |
denotes |
cadmium |
T2784 |
3607-3617 |
JJ |
denotes |
responsive |
T2785 |
3606-3607 |
HYPH |
denotes |
- |
T2786 |
3637-3642 |
NNS |
denotes |
genes |
T2787 |
3617-3619 |
, |
denotes |
, |
T2788 |
3619-3622 |
NN |
denotes |
MTF |
T2789 |
3625-3636 |
JJ |
denotes |
independent |
T2790 |
3622-3623 |
HYPH |
denotes |
- |
T2791 |
3623-3624 |
CD |
denotes |
1 |
T2792 |
3624-3625 |
HYPH |
denotes |
- |
T2793 |
3642-3643 |
. |
denotes |
. |
T2794 |
3643-3832 |
sentence |
denotes |
Genes were considered to be differentially expressed if there was at least a 2-fold difference in expression levels of the compared experimental groups (genotype and respective treatment). |
T2795 |
3644-3649 |
NNS |
denotes |
Genes |
T2796 |
3655-3665 |
VBN |
denotes |
considered |
T2797 |
3650-3654 |
VBD |
denotes |
were |
T2798 |
3666-3668 |
TO |
denotes |
to |
T2799 |
3687-3696 |
VBN |
denotes |
expressed |
T2800 |
3669-3671 |
VB |
denotes |
be |
T2801 |
3672-3686 |
RB |
denotes |
differentially |
T2802 |
3697-3699 |
IN |
denotes |
if |
T2803 |
3706-3709 |
VBD |
denotes |
was |
T2804 |
3700-3705 |
EX |
denotes |
there |
T2805 |
3710-3712 |
RB |
denotes |
at |
T2806 |
3713-3718 |
RBS |
denotes |
least |
T2807 |
3721-3727 |
JJ |
denotes |
2-fold |
T2808 |
3719-3720 |
DT |
denotes |
a |
T2809 |
3728-3738 |
NN |
denotes |
difference |
T2810 |
3739-3741 |
IN |
denotes |
in |
T2811 |
3742-3752 |
NN |
denotes |
expression |
T2812 |
3753-3759 |
NNS |
denotes |
levels |
T2813 |
3760-3762 |
IN |
denotes |
of |
T2814 |
3763-3766 |
DT |
denotes |
the |
T2815 |
3789-3795 |
NNS |
denotes |
groups |
T2816 |
3767-3775 |
VBN |
denotes |
compared |
T2817 |
3776-3788 |
JJ |
denotes |
experimental |
T2818 |
3796-3797 |
-LRB- |
denotes |
( |
T2819 |
3797-3805 |
NN |
denotes |
genotype |
T2820 |
3806-3809 |
CC |
denotes |
and |
T2821 |
3810-3820 |
JJ |
denotes |
respective |
T2822 |
3821-3830 |
NN |
denotes |
treatment |
T2823 |
3830-3831 |
-RRB- |
denotes |
) |
T2824 |
3831-3832 |
. |
denotes |
. |
T2825 |
3832-4017 |
sentence |
denotes |
The result was considered reliable if signal values for the respective gene were scored ‘present’ at least for all mice in one experimental group or for two mice in each of two groups. |
T2826 |
3833-3836 |
DT |
denotes |
The |
T2827 |
3837-3843 |
NN |
denotes |
result |
T2828 |
3848-3858 |
VBN |
denotes |
considered |
T2829 |
3844-3847 |
VBD |
denotes |
was |
T2830 |
3859-3867 |
JJ |
denotes |
reliable |
T2831 |
3868-3870 |
IN |
denotes |
if |
T2832 |
3914-3920 |
VBN |
denotes |
scored |
T2833 |
3871-3877 |
NN |
denotes |
signal |
T2834 |
3878-3884 |
NNS |
denotes |
values |
T2835 |
3885-3888 |
IN |
denotes |
for |
T2836 |
3889-3892 |
DT |
denotes |
the |
T2837 |
3904-3908 |
NN |
denotes |
gene |
T2838 |
3893-3903 |
JJ |
denotes |
respective |
T2839 |
3909-3913 |
VBD |
denotes |
were |
T2840 |
3921-3922 |
`` |
denotes |
‘ |
T2841 |
3922-3929 |
JJ |
denotes |
present |
T2842 |
3929-3930 |
'' |
denotes |
’ |
T2843 |
3931-3933 |
RB |
denotes |
at |
T2844 |
3934-3939 |
RBS |
denotes |
least |
T2845 |
3940-3943 |
IN |
denotes |
for |
T2846 |
3944-3947 |
DT |
denotes |
all |
T2847 |
3948-3952 |
NNS |
denotes |
mice |
T2848 |
3953-3955 |
IN |
denotes |
in |
T2849 |
3956-3959 |
CD |
denotes |
one |
T2850 |
3973-3978 |
NN |
denotes |
group |
T2851 |
3960-3972 |
JJ |
denotes |
experimental |
T2852 |
3979-3981 |
CC |
denotes |
or |
T2853 |
3982-3985 |
IN |
denotes |
for |
T2854 |
3986-3989 |
CD |
denotes |
two |
T2855 |
3990-3994 |
NNS |
denotes |
mice |
T2856 |
3995-3997 |
IN |
denotes |
in |
T2857 |
3998-4002 |
DT |
denotes |
each |
T2858 |
4003-4005 |
IN |
denotes |
of |
T2859 |
4006-4009 |
CD |
denotes |
two |
T2860 |
4010-4016 |
NNS |
denotes |
groups |
T2861 |
4016-4017 |
. |
denotes |
. |
T2862 |
4017-4280 |
sentence |
denotes |
To screen for the presence of the MRE core consensus sequence TGCRCNC in the promoter region, the upstream sequences of the respective genes were obtained from the University of California Santa Cruz (UCSC) Genome Browser Database (; October/November 2004) (28). |
T2863 |
4018-4020 |
TO |
denotes |
To |
T2864 |
4021-4027 |
VB |
denotes |
screen |
T2865 |
4164-4172 |
VBN |
denotes |
obtained |
T2866 |
4028-4031 |
IN |
denotes |
for |
T2867 |
4032-4035 |
DT |
denotes |
the |
T2868 |
4036-4044 |
NN |
denotes |
presence |
T2869 |
4045-4047 |
IN |
denotes |
of |
T2870 |
4048-4051 |
DT |
denotes |
the |
T2871 |
4071-4079 |
NN |
denotes |
sequence |
T2872 |
4052-4055 |
NN |
denotes |
MRE |
T2873 |
4056-4060 |
NN |
denotes |
core |
T2874 |
4061-4070 |
NN |
denotes |
consensus |
T2875 |
4080-4087 |
NN |
denotes |
TGCRCNC |
T2876 |
4088-4090 |
IN |
denotes |
in |
T2877 |
4091-4094 |
DT |
denotes |
the |
T2878 |
4104-4110 |
NN |
denotes |
region |
T2879 |
4095-4103 |
NN |
denotes |
promoter |
T2880 |
4110-4112 |
, |
denotes |
, |
T2881 |
4112-4115 |
DT |
denotes |
the |
T2882 |
4125-4134 |
NNS |
denotes |
sequences |
T2883 |
4116-4124 |
JJ |
denotes |
upstream |
T2884 |
4135-4137 |
IN |
denotes |
of |
T2885 |
4138-4141 |
DT |
denotes |
the |
T2886 |
4153-4158 |
NNS |
denotes |
genes |
T2887 |
4142-4152 |
JJ |
denotes |
respective |
T2888 |
4159-4163 |
VBD |
denotes |
were |
T2889 |
4173-4177 |
IN |
denotes |
from |
T2890 |
4178-4181 |
DT |
denotes |
the |
T2891 |
4240-4248 |
NNP |
denotes |
Database |
T2892 |
4182-4192 |
NNP |
denotes |
University |
T2893 |
4193-4195 |
IN |
denotes |
of |
T2894 |
4196-4206 |
NNP |
denotes |
California |
T2895 |
4213-4217 |
NNP |
denotes |
Cruz |
T2896 |
4207-4212 |
NNP |
denotes |
Santa |
T2897 |
4218-4219 |
-LRB- |
denotes |
( |
T2898 |
4219-4223 |
NNP |
denotes |
UCSC |
T2899 |
4223-4224 |
-RRB- |
denotes |
) |
T2900 |
4225-4231 |
NNP |
denotes |
Genome |
T2901 |
4232-4239 |
NNP |
denotes |
Browser |
T2902 |
4249-4250 |
-LRB- |
denotes |
( |
T2903 |
4260-4268 |
NNP |
denotes |
November |
T2904 |
4250-4251 |
: |
denotes |
; |
T2905 |
4252-4259 |
NNP |
denotes |
October |
T2906 |
4259-4260 |
SYM |
denotes |
/ |
T2907 |
4269-4273 |
CD |
denotes |
2004 |
T2908 |
4273-4274 |
-RRB- |
denotes |
) |
T2909 |
4275-4276 |
-LRB- |
denotes |
( |
T2910 |
4276-4278 |
CD |
denotes |
28 |
T2911 |
4278-4279 |
-RRB- |
denotes |
) |
T2912 |
4279-4280 |
. |
denotes |
. |
T3025 |
4696-4697 |
SYM |
denotes |
′ |
T3026 |
4697-4698 |
HYPH |
denotes |
- |
T3351 |
5514-5529 |
JJ |
denotes |
Electrophoretic |
T3352 |
5539-5544 |
NN |
denotes |
shift |
T3353 |
5530-5538 |
NN |
denotes |
mobility |
T3354 |
5545-5550 |
NN |
denotes |
assay |
T3355 |
5551-5552 |
-LRB- |
denotes |
( |
T3356 |
5552-5556 |
NN |
denotes |
EMSA |
T3357 |
5556-5557 |
-RRB- |
denotes |
) |
T3358 |
5557-5761 |
sentence |
denotes |
Protein was extracted from liver tissue with T-PER™ Tissue Protein Extraction Reagent (Pierce) according to the manufacturer's instructions, using a 1:10 ratio of mouse tissue (mg) to T-PER reagent (µl). |
T3359 |
5558-5565 |
NN |
denotes |
Protein |
T3360 |
5570-5579 |
VBN |
denotes |
extracted |
T3361 |
5566-5569 |
VBD |
denotes |
was |
T3362 |
5580-5584 |
IN |
denotes |
from |
T3363 |
5585-5590 |
NN |
denotes |
liver |
T3364 |
5591-5597 |
NN |
denotes |
tissue |
T3365 |
5598-5602 |
IN |
denotes |
with |
T3366 |
5603-5604 |
NN |
denotes |
T |
T3367 |
5605-5608 |
NN |
denotes |
PER |
T3368 |
5604-5605 |
HYPH |
denotes |
- |
T3369 |
5608-5609 |
SYM |
denotes |
™ |
T3370 |
5610-5616 |
NN |
denotes |
Tissue |
T3371 |
5617-5624 |
NN |
denotes |
Protein |
T3372 |
5625-5635 |
NN |
denotes |
Extraction |
T3373 |
5636-5643 |
NN |
denotes |
Reagent |
T3374 |
5644-5645 |
-LRB- |
denotes |
( |
T3375 |
5645-5651 |
NNP |
denotes |
Pierce |
T3376 |
5651-5652 |
-RRB- |
denotes |
) |
T3377 |
5653-5662 |
VBG |
denotes |
according |
T3378 |
5663-5665 |
IN |
denotes |
to |
T3379 |
5666-5669 |
DT |
denotes |
the |
T3380 |
5670-5682 |
NN |
denotes |
manufacturer |
T3381 |
5685-5697 |
NNS |
denotes |
instructions |
T3382 |
5682-5684 |
POS |
denotes |
's |
T3383 |
5697-5699 |
, |
denotes |
, |
T3384 |
5699-5704 |
VBG |
denotes |
using |
T3385 |
5705-5706 |
DT |
denotes |
a |
T3386 |
5712-5717 |
NN |
denotes |
ratio |
T3387 |
5707-5708 |
CD |
denotes |
1 |
T3388 |
5708-5709 |
SYM |
denotes |
: |
T3389 |
5709-5711 |
CD |
denotes |
10 |
T3390 |
5718-5720 |
IN |
denotes |
of |
T3391 |
5721-5726 |
NN |
denotes |
mouse |
T3392 |
5727-5733 |
NN |
denotes |
tissue |
T3393 |
5734-5735 |
-LRB- |
denotes |
( |
T3394 |
5735-5737 |
NN |
denotes |
mg |
T3395 |
5737-5738 |
-RRB- |
denotes |
) |
T3396 |
5739-5741 |
IN |
denotes |
to |
T3397 |
5742-5743 |
NN |
denotes |
T |
T3398 |
5744-5747 |
NN |
denotes |
PER |
T3399 |
5743-5744 |
HYPH |
denotes |
- |
T3400 |
5748-5755 |
NN |
denotes |
reagent |
T3401 |
5756-5757 |
-LRB- |
denotes |
( |
T3402 |
5757-5759 |
NN |
denotes |
µl |
T3403 |
5759-5760 |
-RRB- |
denotes |
) |
T3404 |
5760-5761 |
. |
denotes |
. |
T3405 |
5761-5822 |
sentence |
denotes |
EMSA was essentially performed as previously described (10). |
T3406 |
5762-5766 |
NN |
denotes |
EMSA |
T3407 |
5783-5792 |
VBN |
denotes |
performed |
T3408 |
5767-5770 |
VBD |
denotes |
was |
T3409 |
5771-5782 |
RB |
denotes |
essentially |
T3410 |
5793-5795 |
IN |
denotes |
as |
T3411 |
5807-5816 |
VBN |
denotes |
described |
T3412 |
5796-5806 |
RB |
denotes |
previously |
T3413 |
5817-5818 |
-LRB- |
denotes |
( |
T3414 |
5818-5820 |
CD |
denotes |
10 |
T3415 |
5820-5821 |
-RRB- |
denotes |
) |
T3416 |
5821-5822 |
. |
denotes |
. |
T3417 |
5822-5975 |
sentence |
denotes |
All binding reactions were carried out by incubating 2–5 fmol 32P-end-labeled double-stranded oligonucleotides with 100 to 130 µg liver protein extract. |
T3418 |
5823-5826 |
DT |
denotes |
All |
T3419 |
5835-5844 |
NNS |
denotes |
reactions |
T3420 |
5827-5834 |
NN |
denotes |
binding |
T3421 |
5850-5857 |
VBN |
denotes |
carried |
T3422 |
5845-5849 |
VBD |
denotes |
were |
T3423 |
5858-5861 |
RP |
denotes |
out |
T3424 |
5862-5864 |
IN |
denotes |
by |
T3425 |
5865-5875 |
VBG |
denotes |
incubating |
T3426 |
5876-5877 |
CD |
denotes |
2 |
T3427 |
5878-5879 |
CD |
denotes |
5 |
T3428 |
5877-5878 |
SYM |
denotes |
– |
T3429 |
5880-5884 |
NN |
denotes |
fmol |
T3430 |
5917-5933 |
NNS |
denotes |
oligonucleotides |
T3431 |
5885-5888 |
NN |
denotes |
32P |
T3432 |
5893-5900 |
VBN |
denotes |
labeled |
T3433 |
5888-5889 |
HYPH |
denotes |
- |
T3434 |
5889-5892 |
NN |
denotes |
end |
T3435 |
5892-5893 |
HYPH |
denotes |
- |
T3436 |
5901-5907 |
JJ |
denotes |
double |
T3437 |
5908-5916 |
VBN |
denotes |
stranded |
T3438 |
5907-5908 |
HYPH |
denotes |
- |
T3439 |
5934-5938 |
IN |
denotes |
with |
T3440 |
5939-5942 |
CD |
denotes |
100 |
T3441 |
5946-5949 |
CD |
denotes |
130 |
T3442 |
5943-5945 |
IN |
denotes |
to |
T3443 |
5950-5952 |
NN |
denotes |
µg |
T3444 |
5967-5974 |
NN |
denotes |
extract |
T3445 |
5953-5958 |
NN |
denotes |
liver |
T3446 |
5959-5966 |
NN |
denotes |
protein |
T3447 |
5974-5975 |
. |
denotes |
. |
T3448 |
5975-6117 |
sentence |
denotes |
For competition experiments, 5 pmol of unlabeled competitor oligonucleotide was added to the binding reaction before addition of the extract. |
T3449 |
5976-5979 |
IN |
denotes |
For |
T3450 |
6056-6061 |
VBN |
denotes |
added |
T3451 |
5980-5991 |
NN |
denotes |
competition |
T3452 |
5992-6003 |
NNS |
denotes |
experiments |
T3453 |
6003-6005 |
, |
denotes |
, |
T3454 |
6005-6006 |
CD |
denotes |
5 |
T3455 |
6007-6011 |
NN |
denotes |
pmol |
T3456 |
6012-6014 |
IN |
denotes |
of |
T3457 |
6015-6024 |
JJ |
denotes |
unlabeled |
T3458 |
6036-6051 |
NN |
denotes |
oligonucleotide |
T3459 |
6025-6035 |
NN |
denotes |
competitor |
T3460 |
6052-6055 |
VBD |
denotes |
was |
T3461 |
6062-6064 |
IN |
denotes |
to |
T3462 |
6065-6068 |
DT |
denotes |
the |
T3463 |
6077-6085 |
NN |
denotes |
reaction |
T3464 |
6069-6076 |
NN |
denotes |
binding |
T3465 |
6086-6092 |
IN |
denotes |
before |
T3466 |
6093-6101 |
NN |
denotes |
addition |
T3467 |
6102-6104 |
IN |
denotes |
of |
T3468 |
6105-6108 |
DT |
denotes |
the |
T3469 |
6109-6116 |
NN |
denotes |
extract |
T3470 |
6116-6117 |
. |
denotes |
. |
T3471 |
6117-6189 |
sentence |
denotes |
All EMSA gels were developed using PhosphorImager (Molecular Dynamics). |
T3472 |
6118-6121 |
DT |
denotes |
All |
T3473 |
6127-6131 |
NNS |
denotes |
gels |
T3474 |
6122-6126 |
NN |
denotes |
EMSA |
T3475 |
6137-6146 |
VBN |
denotes |
developed |
T3476 |
6132-6136 |
VBD |
denotes |
were |
T3477 |
6147-6152 |
VBG |
denotes |
using |
T3478 |
6153-6167 |
NNP |
denotes |
PhosphorImager |
T3479 |
6168-6169 |
-LRB- |
denotes |
( |
T3480 |
6179-6187 |
NNP |
denotes |
Dynamics |
T3481 |
6169-6178 |
NNP |
denotes |
Molecular |
T3482 |
6187-6188 |
-RRB- |
denotes |
) |
T3483 |
6188-6189 |
. |
denotes |
. |
T3484 |
6189-7651 |
sentence |
denotes |
The following oligonucleotides were annealed and used for the reactions: Csrp1 MRE1: 5′-GGAAACAAAACGGCGCGCACTCCGGCGC-3′; 5′-GGCTGCGC CGGAGTGCGCGCCGTTTTGT-3′, Csrp1 MRE2: 5′-TGTTGTGGTGCAGTGTGCAAAGCCTAC-3′; 5′-ACCAGTAGGCTTTGCACACTGCACCAC-3′, Csrp1 MRE3: 5′-GAGATCGCCATAGGGTGCAAAGAGAAG-3′; 5′-GTGACTTCTCTTTGCACCCTATGGCGA-3′, Csrp1 MRE4: 5′-TGTCTTATTCTGGAGTGCAAGTTAGTC-3′; 5′-AGGGGACTAACTTGCACTCCAGAATAA-3′, Gal4: 5′-TCCGGAGGACTGTCCTCCGG-3′; 5′-GCCGGAGGACAGTCCTCCGG-3′, MREd [MRE derived from mouse Mt1 promoter (10)]: 5′-CGAGGGAGCTCTGCACTCCGCCCGAAAAGTG-3′; 5′-TCGACACTTTTCGGGCGGAGTGCAGAGCTCCCTCGAGCT-3′, MRE-s [synthetic MRE consensus sequence (10)]: 5′-CGAGGGAGCTCTGCACACGGCCCGAAAAGTG-3′; 5′-TCGACACTTTTCGGGCCGTGTGCAGAGCTCCCTCGAGCT-3′, Ndrg1 MRE1: 5′-CAGCCCAGGCAGGGTGCAGCACGAG-3′; 5′-CCGCCTCGTGCTGCACCCTGCCTGG-3′, Ndrg1 MRE2: 5′-CACACGTTCGCTGCACACGCCGCGG-3′; 5′-GGGACCGCGGCGTGTGCAGCGAACG-3′, Ndrg1 MRE3,4: 5′-GGAGTCCTTATGCACACGCGCACGAGCGCGCACGGGCAC-3′; 5′-TGGTGTGCCCGTGCGCGCTCGTGCGCGTGTGCATAAGGAC-3′, Sepw1 MRE1: 5′-GAGGCAGTCGGCTGTGCGCACGGCCCCACGCTC-3′; 5′-CTCTGAGCGTGGGGCCGTGCGCACAGCCGACTGC-3′, Sepw1 MRE2: 5′-ATGGTTTTGGGGGTGCGCAGGGGGTCTG-3′; 5′-CGACAGACCCCCTGCGCACCCCCAAAAC-3′, Slc39a10 MRE1: 5′-GAATACACGACTGGGTGCAGCCGGGGTTTGG-3′; 5′-GGTACCAAACCCCGGCTGCACCCAGTCGTGTA-3′, Slc39a10 MRE2: 5′-GCGGAGAGGAGATGCACACGGCACTCG-3′; 5′-CACTCGAGTGCCGTGTGCATCTCCTCT-3′, Specificity protein 1 (Sp1) binding sequence (10): 5′-CGAGGCCCCGCCCAG-3′; 5′-TCGACTGGGCGGGGCCTCGAGct-3′. |
T3485 |
6190-6193 |
DT |
denotes |
The |
T3486 |
6204-6220 |
NNS |
denotes |
oligonucleotides |
T3487 |
6194-6203 |
VBG |
denotes |
following |
T3488 |
6226-6234 |
VBN |
denotes |
annealed |
T3489 |
6221-6225 |
VBD |
denotes |
were |
T3490 |
6235-6238 |
CC |
denotes |
and |
T3491 |
6239-6243 |
VBN |
denotes |
used |
T3492 |
6244-6247 |
IN |
denotes |
for |
T3493 |
6248-6251 |
DT |
denotes |
the |
T3494 |
6252-6261 |
NNS |
denotes |
reactions |
T3495 |
6261-6263 |
: |
denotes |
: |
T3496 |
6263-6268 |
NN |
denotes |
Csrp1 |
T3497 |
6269-6273 |
NN |
denotes |
MRE1 |
T3498 |
6273-6275 |
: |
denotes |
: |
T3499 |
6275-6276 |
CD |
denotes |
5 |
T3500 |
6278-6306 |
NN |
denotes |
GGAAACAAAACGGCGCGCACTCCGGCGC |
T3501 |
6276-6277 |
SYM |
denotes |
′ |
T3502 |
6277-6278 |
HYPH |
denotes |
- |
T3503 |
6306-6307 |
HYPH |
denotes |
- |
T3504 |
6307-6308 |
CD |
denotes |
3 |
T3505 |
6308-6309 |
SYM |
denotes |
′ |
T3506 |
6309-6310 |
: |
denotes |
; |
T3507 |
6311-6312 |
CD |
denotes |
5 |
T3508 |
6323-6343 |
NN |
denotes |
CGGAGTGCGCGCCGTTTTGT |
T3509 |
6312-6313 |
SYM |
denotes |
′ |
T3510 |
6313-6314 |
HYPH |
denotes |
- |
T3511 |
6314-6322 |
NN |
denotes |
GGCTGCGC |
T3512 |
6343-6344 |
HYPH |
denotes |
- |
T3513 |
6344-6345 |
CD |
denotes |
3 |
T3514 |
6345-6346 |
SYM |
denotes |
′ |
T3515 |
6346-6348 |
, |
denotes |
, |
T3516 |
6348-6353 |
NN |
denotes |
Csrp1 |
T3517 |
6354-6358 |
NN |
denotes |
MRE2 |
T3518 |
6358-6360 |
: |
denotes |
: |
T3519 |
6360-6361 |
CD |
denotes |
5 |
T3520 |
6363-6390 |
NN |
denotes |
TGTTGTGGTGCAGTGTGCAAAGCCTAC |
T3521 |
6361-6362 |
SYM |
denotes |
′ |
T3522 |
6362-6363 |
HYPH |
denotes |
- |
T3523 |
6390-6391 |
HYPH |
denotes |
- |
T3524 |
6391-6392 |
CD |
denotes |
3 |
T3525 |
6392-6393 |
SYM |
denotes |
′ |
T3526 |
6393-6394 |
: |
denotes |
; |
T3527 |
6395-6396 |
CD |
denotes |
5 |
T3528 |
6398-6425 |
NN |
denotes |
ACCAGTAGGCTTTGCACACTGCACCAC |
T3529 |
6396-6397 |
SYM |
denotes |
′ |
T3530 |
6397-6398 |
HYPH |
denotes |
- |
T3531 |
6425-6426 |
HYPH |
denotes |
- |
T3532 |
6426-6427 |
CD |
denotes |
3 |
T3533 |
6427-6428 |
SYM |
denotes |
′ |
T3534 |
6428-6430 |
, |
denotes |
, |
T3535 |
6430-6435 |
NN |
denotes |
Csrp1 |
T3536 |
6436-6440 |
NN |
denotes |
MRE3 |
T3537 |
6440-6442 |
: |
denotes |
: |
T3538 |
6442-6443 |
CD |
denotes |
5 |
T3539 |
6445-6472 |
NN |
denotes |
GAGATCGCCATAGGGTGCAAAGAGAAG |
T3540 |
6443-6444 |
SYM |
denotes |
′ |
T3541 |
6444-6445 |
HYPH |
denotes |
- |
T3542 |
6472-6473 |
HYPH |
denotes |
- |
T3543 |
6473-6474 |
CD |
denotes |
3 |
T3544 |
6474-6475 |
SYM |
denotes |
′ |
T3545 |
6475-6476 |
: |
denotes |
; |
T3546 |
6477-6478 |
CD |
denotes |
5 |
T3547 |
6480-6507 |
NN |
denotes |
GTGACTTCTCTTTGCACCCTATGGCGA |
T3548 |
6478-6479 |
SYM |
denotes |
′ |
T3549 |
6479-6480 |
HYPH |
denotes |
- |
T3550 |
6507-6508 |
HYPH |
denotes |
- |
T3551 |
6508-6509 |
CD |
denotes |
3 |
T3552 |
6509-6510 |
SYM |
denotes |
′ |
T3553 |
6510-6512 |
, |
denotes |
, |
T3554 |
6512-6517 |
NN |
denotes |
Csrp1 |
T3555 |
6518-6522 |
NN |
denotes |
MRE4 |
T3556 |
6522-6524 |
: |
denotes |
: |
T3557 |
6524-6525 |
CD |
denotes |
5 |
T3558 |
6527-6554 |
NN |
denotes |
TGTCTTATTCTGGAGTGCAAGTTAGTC |
T3559 |
6525-6526 |
SYM |
denotes |
′ |
T3560 |
6526-6527 |
HYPH |
denotes |
- |
T3561 |
6554-6555 |
HYPH |
denotes |
- |
T3562 |
6555-6556 |
CD |
denotes |
3 |
T3563 |
6556-6557 |
SYM |
denotes |
′ |
T3564 |
6557-6558 |
: |
denotes |
; |
T3565 |
6559-6560 |
CD |
denotes |
5 |
T3566 |
6562-6589 |
NN |
denotes |
AGGGGACTAACTTGCACTCCAGAATAA |
T3567 |
6560-6561 |
SYM |
denotes |
′ |
T3568 |
6561-6562 |
HYPH |
denotes |
- |
T3569 |
6589-6590 |
HYPH |
denotes |
- |
T3570 |
6590-6591 |
CD |
denotes |
3 |
T3571 |
6591-6592 |
SYM |
denotes |
′ |
T3572 |
6592-6594 |
, |
denotes |
, |
T3573 |
6594-6598 |
NN |
denotes |
Gal4 |
T3574 |
6598-6600 |
: |
denotes |
: |
T3575 |
6600-6601 |
CD |
denotes |
5 |
T3576 |
6603-6623 |
NN |
denotes |
TCCGGAGGACTGTCCTCCGG |
T3577 |
6601-6602 |
SYM |
denotes |
′ |
T3578 |
6602-6603 |
HYPH |
denotes |
- |
T3579 |
6623-6624 |
HYPH |
denotes |
- |
T3580 |
6624-6625 |
CD |
denotes |
3 |
T3581 |
6625-6626 |
SYM |
denotes |
′ |
T3582 |
6626-6627 |
: |
denotes |
; |
T3583 |
6628-6629 |
CD |
denotes |
5 |
T3584 |
6631-6651 |
NN |
denotes |
GCCGGAGGACAGTCCTCCGG |
T3585 |
6629-6630 |
SYM |
denotes |
′ |
T3586 |
6630-6631 |
HYPH |
denotes |
- |
T3587 |
6651-6652 |
HYPH |
denotes |
- |
T3588 |
6652-6653 |
CD |
denotes |
3 |
T3589 |
6653-6654 |
SYM |
denotes |
′ |
T3590 |
6654-6656 |
, |
denotes |
, |
T3591 |
6656-6660 |
NN |
denotes |
MREd |
T3592 |
6661-6662 |
-LRB- |
denotes |
[ |
T3593 |
6662-6665 |
NN |
denotes |
MRE |
T3594 |
6666-6673 |
VBN |
denotes |
derived |
T3595 |
6674-6678 |
IN |
denotes |
from |
T3596 |
6679-6684 |
NN |
denotes |
mouse |
T3597 |
6689-6697 |
NN |
denotes |
promoter |
T3598 |
6685-6688 |
NN |
denotes |
Mt1 |
T3599 |
6698-6699 |
-LRB- |
denotes |
( |
T3600 |
6699-6701 |
CD |
denotes |
10 |
T3601 |
6701-6702 |
-RRB- |
denotes |
) |
T3602 |
6702-6703 |
-RRB- |
denotes |
] |
T3603 |
6703-6705 |
: |
denotes |
: |
T3604 |
6705-6706 |
CD |
denotes |
5 |
T3605 |
6708-6739 |
NN |
denotes |
CGAGGGAGCTCTGCACTCCGCCCGAAAAGTG |
T3606 |
6706-6707 |
SYM |
denotes |
′ |
T3607 |
6707-6708 |
HYPH |
denotes |
- |
T3608 |
6739-6740 |
HYPH |
denotes |
- |
T3609 |
6740-6741 |
CD |
denotes |
3 |
T3610 |
6741-6742 |
SYM |
denotes |
′ |
T3611 |
6742-6743 |
: |
denotes |
; |
T3612 |
6744-6745 |
CD |
denotes |
5 |
T3613 |
6747-6786 |
NN |
denotes |
TCGACACTTTTCGGGCGGAGTGCAGAGCTCCCTCGAGCT |
T3614 |
6745-6746 |
SYM |
denotes |
′ |
T3615 |
6746-6747 |
HYPH |
denotes |
- |
T3616 |
6786-6787 |
HYPH |
denotes |
- |
T3617 |
6787-6788 |
CD |
denotes |
3 |
T3618 |
6788-6789 |
SYM |
denotes |
′ |
T3619 |
6789-6791 |
, |
denotes |
, |
T3620 |
6791-6794 |
NN |
denotes |
MRE |
T3621 |
6795-6796 |
NN |
denotes |
s |
T3622 |
6794-6795 |
HYPH |
denotes |
- |
T3623 |
6797-6798 |
-LRB- |
denotes |
[ |
T3624 |
6798-6807 |
JJ |
denotes |
synthetic |
T3625 |
6822-6830 |
NN |
denotes |
sequence |
T3626 |
6808-6811 |
NN |
denotes |
MRE |
T3627 |
6812-6821 |
NN |
denotes |
consensus |
T3628 |
6831-6832 |
-LRB- |
denotes |
( |
T3629 |
6832-6834 |
CD |
denotes |
10 |
T3630 |
6834-6835 |
-RRB- |
denotes |
) |
T3631 |
6835-6836 |
-RRB- |
denotes |
] |
T3632 |
6836-6838 |
: |
denotes |
: |
T3633 |
6838-6839 |
CD |
denotes |
5 |
T3634 |
6841-6872 |
NN |
denotes |
CGAGGGAGCTCTGCACACGGCCCGAAAAGTG |
T3635 |
6839-6840 |
SYM |
denotes |
′ |
T3636 |
6840-6841 |
HYPH |
denotes |
- |
T3637 |
6872-6873 |
HYPH |
denotes |
- |
T3638 |
6873-6874 |
CD |
denotes |
3 |
T3639 |
6874-6875 |
SYM |
denotes |
′ |
T3640 |
6875-6876 |
: |
denotes |
; |
T3641 |
6877-6878 |
CD |
denotes |
5 |
T3642 |
6880-6919 |
NN |
denotes |
TCGACACTTTTCGGGCCGTGTGCAGAGCTCCCTCGAGCT |
T3643 |
6878-6879 |
SYM |
denotes |
′ |
T3644 |
6879-6880 |
HYPH |
denotes |
- |
T3645 |
6919-6920 |
HYPH |
denotes |
- |
T3646 |
6920-6921 |
CD |
denotes |
3 |
T3647 |
6921-6922 |
SYM |
denotes |
′ |
T3648 |
6922-6924 |
, |
denotes |
, |
T3649 |
6924-6929 |
NN |
denotes |
Ndrg1 |
T3650 |
6930-6934 |
NN |
denotes |
MRE1 |
T3651 |
6934-6936 |
: |
denotes |
: |
T3652 |
6936-6937 |
CD |
denotes |
5 |
T3653 |
6939-6964 |
NN |
denotes |
CAGCCCAGGCAGGGTGCAGCACGAG |
T3654 |
6937-6938 |
SYM |
denotes |
′ |
T3655 |
6938-6939 |
HYPH |
denotes |
- |
T3656 |
6964-6965 |
HYPH |
denotes |
- |
T3657 |
6965-6966 |
CD |
denotes |
3 |
T3658 |
6966-6967 |
SYM |
denotes |
′ |
T3659 |
6967-6968 |
: |
denotes |
; |
T3660 |
6969-6970 |
CD |
denotes |
5 |
T3661 |
6972-6997 |
NN |
denotes |
CCGCCTCGTGCTGCACCCTGCCTGG |
T3662 |
6970-6971 |
SYM |
denotes |
′ |
T3663 |
6971-6972 |
HYPH |
denotes |
- |
T3664 |
6997-6998 |
HYPH |
denotes |
- |
T3665 |
6998-6999 |
CD |
denotes |
3 |
T3666 |
6999-7000 |
SYM |
denotes |
′ |
T3667 |
7000-7002 |
, |
denotes |
, |
T3668 |
7002-7007 |
NN |
denotes |
Ndrg1 |
T3669 |
7008-7012 |
NN |
denotes |
MRE2 |
T3670 |
7012-7014 |
: |
denotes |
: |
T3671 |
7014-7015 |
CD |
denotes |
5 |
T3672 |
7017-7042 |
NN |
denotes |
CACACGTTCGCTGCACACGCCGCGG |
T3673 |
7015-7016 |
SYM |
denotes |
′ |
T3674 |
7016-7017 |
HYPH |
denotes |
- |
T3675 |
7042-7043 |
HYPH |
denotes |
- |
T3676 |
7043-7044 |
CD |
denotes |
3 |
T3677 |
7044-7045 |
SYM |
denotes |
′ |
T3678 |
7045-7046 |
: |
denotes |
; |
T3679 |
7047-7048 |
CD |
denotes |
5 |
T3680 |
7050-7075 |
NN |
denotes |
GGGACCGCGGCGTGTGCAGCGAACG |
T3681 |
7048-7049 |
SYM |
denotes |
′ |
T3682 |
7049-7050 |
HYPH |
denotes |
- |
T3683 |
7075-7076 |
HYPH |
denotes |
- |
T3684 |
7076-7077 |
CD |
denotes |
3 |
T3685 |
7077-7078 |
SYM |
denotes |
′ |
T3686 |
7078-7080 |
, |
denotes |
, |
T3687 |
7080-7085 |
NN |
denotes |
Ndrg1 |
T3688 |
7086-7090 |
NN |
denotes |
MRE3 |
T3689 |
7090-7091 |
, |
denotes |
, |
T3690 |
7091-7092 |
CD |
denotes |
4 |
T3691 |
7092-7094 |
: |
denotes |
: |
T3692 |
7094-7095 |
CD |
denotes |
5 |
T3693 |
7097-7136 |
NN |
denotes |
GGAGTCCTTATGCACACGCGCACGAGCGCGCACGGGCAC |
T3694 |
7095-7096 |
SYM |
denotes |
′ |
T3695 |
7096-7097 |
HYPH |
denotes |
- |
T3696 |
7136-7137 |
HYPH |
denotes |
- |
T3697 |
7137-7138 |
CD |
denotes |
3 |
T3698 |
7138-7139 |
SYM |
denotes |
′ |
T3699 |
7139-7140 |
: |
denotes |
; |
T3700 |
7141-7142 |
CD |
denotes |
5 |
T3701 |
7144-7184 |
NN |
denotes |
TGGTGTGCCCGTGCGCGCTCGTGCGCGTGTGCATAAGGAC |
T3702 |
7142-7143 |
SYM |
denotes |
′ |
T3703 |
7143-7144 |
HYPH |
denotes |
- |
T3704 |
7184-7185 |
HYPH |
denotes |
- |
T3705 |
7185-7186 |
CD |
denotes |
3 |
T3706 |
7186-7187 |
SYM |
denotes |
′ |
T3707 |
7187-7189 |
, |
denotes |
, |
T3708 |
7189-7194 |
NN |
denotes |
Sepw1 |
T3709 |
7195-7199 |
NN |
denotes |
MRE1 |
T3710 |
7199-7201 |
: |
denotes |
: |
T3711 |
7201-7202 |
CD |
denotes |
5 |
T3712 |
7204-7237 |
NN |
denotes |
GAGGCAGTCGGCTGTGCGCACGGCCCCACGCTC |
T3713 |
7202-7203 |
SYM |
denotes |
′ |
T3714 |
7203-7204 |
HYPH |
denotes |
- |
T3715 |
7237-7238 |
HYPH |
denotes |
- |
T3716 |
7238-7239 |
CD |
denotes |
3 |
T3717 |
7239-7240 |
SYM |
denotes |
′ |
T3718 |
7240-7241 |
: |
denotes |
; |
T3719 |
7242-7243 |
CD |
denotes |
5 |
T3720 |
7245-7279 |
NN |
denotes |
CTCTGAGCGTGGGGCCGTGCGCACAGCCGACTGC |
T3721 |
7243-7244 |
SYM |
denotes |
′ |
T3722 |
7244-7245 |
HYPH |
denotes |
- |
T3723 |
7279-7280 |
HYPH |
denotes |
- |
T3724 |
7280-7281 |
CD |
denotes |
3 |
T3725 |
7281-7282 |
SYM |
denotes |
′ |
T3726 |
7282-7284 |
, |
denotes |
, |
T3727 |
7284-7289 |
NN |
denotes |
Sepw1 |
T3728 |
7290-7294 |
NN |
denotes |
MRE2 |
T3729 |
7294-7296 |
: |
denotes |
: |
T3730 |
7296-7297 |
CD |
denotes |
5 |
T3731 |
7299-7327 |
NN |
denotes |
ATGGTTTTGGGGGTGCGCAGGGGGTCTG |
T3732 |
7297-7298 |
SYM |
denotes |
′ |
T3733 |
7298-7299 |
HYPH |
denotes |
- |
T3734 |
7327-7328 |
HYPH |
denotes |
- |
T3735 |
7328-7329 |
CD |
denotes |
3 |
T3736 |
7329-7330 |
SYM |
denotes |
′ |
T3737 |
7330-7331 |
: |
denotes |
; |
T3738 |
7332-7333 |
CD |
denotes |
5 |
T3739 |
7335-7363 |
NN |
denotes |
CGACAGACCCCCTGCGCACCCCCAAAAC |
T3740 |
7333-7334 |
SYM |
denotes |
′ |
T3741 |
7334-7335 |
HYPH |
denotes |
- |
T3742 |
7363-7364 |
HYPH |
denotes |
- |
T3743 |
7364-7365 |
CD |
denotes |
3 |
T3744 |
7365-7366 |
SYM |
denotes |
′ |
T3745 |
7366-7368 |
, |
denotes |
, |
T3746 |
7368-7376 |
NN |
denotes |
Slc39a10 |
T3747 |
7377-7381 |
NN |
denotes |
MRE1 |
T3748 |
7381-7383 |
: |
denotes |
: |
T3749 |
7383-7384 |
CD |
denotes |
5 |
T3750 |
7386-7417 |
NN |
denotes |
GAATACACGACTGGGTGCAGCCGGGGTTTGG |
T3751 |
7384-7385 |
SYM |
denotes |
′ |
T3752 |
7385-7386 |
HYPH |
denotes |
- |
T3753 |
7417-7418 |
HYPH |
denotes |
- |
T3754 |
7418-7419 |
CD |
denotes |
3 |
T3755 |
7419-7420 |
SYM |
denotes |
′ |
T3756 |
7420-7421 |
: |
denotes |
; |
T3757 |
7422-7423 |
CD |
denotes |
5 |
T3758 |
7425-7457 |
NN |
denotes |
GGTACCAAACCCCGGCTGCACCCAGTCGTGTA |
T3759 |
7423-7424 |
SYM |
denotes |
′ |
T3760 |
7424-7425 |
HYPH |
denotes |
- |
T3761 |
7457-7458 |
HYPH |
denotes |
- |
T3762 |
7458-7459 |
CD |
denotes |
3 |
T3763 |
7459-7460 |
SYM |
denotes |
′ |
T3764 |
7460-7462 |
, |
denotes |
, |
T3765 |
7462-7470 |
NN |
denotes |
Slc39a10 |
T3766 |
7471-7475 |
NN |
denotes |
MRE2 |
T3767 |
7475-7477 |
: |
denotes |
: |
T3768 |
7477-7478 |
CD |
denotes |
5 |
T3769 |
7480-7507 |
NN |
denotes |
GCGGAGAGGAGATGCACACGGCACTCG |
T3770 |
7478-7479 |
SYM |
denotes |
′ |
T3771 |
7479-7480 |
HYPH |
denotes |
- |
T3772 |
7507-7508 |
HYPH |
denotes |
- |
T3773 |
7508-7509 |
CD |
denotes |
3 |
T3774 |
7509-7510 |
SYM |
denotes |
′ |
T3775 |
7510-7511 |
: |
denotes |
; |
T3776 |
7512-7513 |
CD |
denotes |
5 |
T3777 |
7515-7542 |
NN |
denotes |
CACTCGAGTGCCGTGTGCATCTCCTCT |
T3778 |
7513-7514 |
SYM |
denotes |
′ |
T3779 |
7514-7515 |
HYPH |
denotes |
- |
T3780 |
7542-7543 |
HYPH |
denotes |
- |
T3781 |
7543-7544 |
CD |
denotes |
3 |
T3782 |
7544-7545 |
SYM |
denotes |
′ |
T3783 |
7545-7547 |
, |
denotes |
, |
T3784 |
7547-7558 |
NN |
denotes |
Specificity |
T3785 |
7559-7566 |
NN |
denotes |
protein |
T3786 |
7583-7591 |
NN |
denotes |
sequence |
T3787 |
7567-7568 |
CD |
denotes |
1 |
T3788 |
7569-7570 |
-LRB- |
denotes |
( |
T3789 |
7570-7573 |
NN |
denotes |
Sp1 |
T3790 |
7573-7574 |
-RRB- |
denotes |
) |
T3791 |
7575-7582 |
NN |
denotes |
binding |
T3792 |
7592-7593 |
-LRB- |
denotes |
( |
T3793 |
7593-7595 |
CD |
denotes |
10 |
T3794 |
7595-7596 |
-RRB- |
denotes |
) |
T3795 |
7596-7598 |
: |
denotes |
: |
T3796 |
7598-7599 |
CD |
denotes |
5 |
T3797 |
7601-7616 |
NN |
denotes |
CGAGGCCCCGCCCAG |
T3798 |
7599-7600 |
SYM |
denotes |
′ |
T3799 |
7600-7601 |
HYPH |
denotes |
- |
T3800 |
7616-7617 |
HYPH |
denotes |
- |
T3801 |
7617-7618 |
CD |
denotes |
3 |
T3802 |
7618-7619 |
SYM |
denotes |
′ |
T3803 |
7619-7620 |
: |
denotes |
; |
T3804 |
7621-7622 |
CD |
denotes |
5 |
T3805 |
7624-7647 |
NN |
denotes |
TCGACTGGGCGGGGCCTCGAGct |
T3806 |
7622-7623 |
SYM |
denotes |
′ |
T3807 |
7623-7624 |
HYPH |
denotes |
- |
T3808 |
7647-7648 |
HYPH |
denotes |
- |
T3809 |
7648-7649 |
CD |
denotes |
3 |
T3810 |
7649-7650 |
SYM |
denotes |
′ |
T3811 |
7650-7651 |
. |
denotes |
. |
T3948 |
8010-8011 |
-LRB- |
denotes |
( |
T3949 |
8011-8015 |
NN |
denotes |
SV40 |
T3950 |
8015-8016 |
-RRB- |
denotes |
) |
T3951 |
8017-8022 |
JJ |
denotes |
large |
T3952 |
8023-8024 |
NN |
denotes |
T |
T3953 |
8033-8039 |
VBN |
denotes |
driven |
T3954 |
8040-8042 |
IN |
denotes |
by |
T3955 |
8043-8046 |
DT |
denotes |
the |
T3956 |
8069-8077 |
NN |
denotes |
promoter |
T3957 |
8047-8062 |
NN |
denotes |
cytomegalovirus |
T3958 |
8063-8064 |
-LRB- |
denotes |
( |
T3959 |
8064-8067 |
NN |
denotes |
CMV |
T3960 |
8067-8068 |
-RRB- |
denotes |
) |
T3961 |
8077-8079 |
, |
denotes |
, |
T3962 |
8079-8084 |
VBG |
denotes |
using |
T3963 |
8085-8098 |
NNP |
denotes |
lipofectamine |
T3964 |
8100-8107 |
NN |
denotes |
reagent |
T3965 |
8098-8099 |
SYM |
denotes |
™ |
T3966 |
8108-8109 |
-LRB- |
denotes |
( |
T3967 |
8109-8119 |
NNP |
denotes |
Invitrogen |
T3968 |
8119-8120 |
-RRB- |
denotes |
) |
T3969 |
8121-8130 |
VBG |
denotes |
according |
T3970 |
8131-8133 |
IN |
denotes |
to |
T3971 |
8134-8137 |
DT |
denotes |
the |
T3972 |
8138-8150 |
NN |
denotes |
manufacturer |
T3973 |
8153-8165 |
NNS |
denotes |
instructions |
T3974 |
8150-8152 |
POS |
denotes |
's |
T3975 |
8165-8166 |
. |
denotes |
. |
T3976 |
8166-8283 |
sentence |
denotes |
Cell foci were isolated and the immortalized mouse embryonic fibroblast cell line ckoC was derived from one of them. |
T3977 |
8167-8171 |
NN |
denotes |
Cell |
T3978 |
8172-8176 |
NNS |
denotes |
foci |
T3979 |
8182-8190 |
VBN |
denotes |
isolated |
T3980 |
8177-8181 |
VBD |
denotes |
were |
T3981 |
8191-8194 |
CC |
denotes |
and |
T3982 |
8195-8198 |
DT |
denotes |
the |
T3983 |
8244-8248 |
NN |
denotes |
line |
T3984 |
8199-8211 |
VBN |
denotes |
immortalized |
T3985 |
8212-8217 |
NN |
denotes |
mouse |
T3986 |
8228-8238 |
NN |
denotes |
fibroblast |
T3987 |
8218-8227 |
JJ |
denotes |
embryonic |
T3988 |
8239-8243 |
NN |
denotes |
cell |
T3989 |
8258-8265 |
VBN |
denotes |
derived |
T3990 |
8249-8253 |
NN |
denotes |
ckoC |
T3991 |
8254-8257 |
VBD |
denotes |
was |
T3992 |
8266-8270 |
IN |
denotes |
from |
T3993 |
8271-8274 |
CD |
denotes |
one |
T3994 |
8275-8277 |
IN |
denotes |
of |
T3995 |
8278-8282 |
PRP |
denotes |
them |
T3996 |
8282-8283 |
. |
denotes |
. |
T3997 |
8283-8393 |
sentence |
denotes |
The Mtf1loxP genotype of this line as well as the genomic integration of the T antigen were confirmed by PCR. |
T3998 |
8284-8287 |
DT |
denotes |
The |
T3999 |
8297-8305 |
NN |
denotes |
genotype |
T4000 |
8288-8296 |
NN |
denotes |
Mtf1loxP |
T4001 |
8376-8385 |
VBN |
denotes |
confirmed |
T4002 |
8306-8308 |
IN |
denotes |
of |
T4003 |
8309-8313 |
DT |
denotes |
this |
T4004 |
8314-8318 |
NN |
denotes |
line |
T4005 |
8319-8321 |
RB |
denotes |
as |
T4006 |
8327-8329 |
IN |
denotes |
as |
T4007 |
8322-8326 |
RB |
denotes |
well |
T4008 |
8330-8333 |
DT |
denotes |
the |
T4009 |
8342-8353 |
NN |
denotes |
integration |
T4010 |
8334-8341 |
JJ |
denotes |
genomic |
T4011 |
8354-8356 |
IN |
denotes |
of |
T4012 |
8357-8360 |
DT |
denotes |
the |
T4013 |
8363-8370 |
NN |
denotes |
antigen |
T4014 |
8361-8362 |
NN |
denotes |
T |
T4015 |
8371-8375 |
VBD |
denotes |
were |
T4016 |
8386-8388 |
IN |
denotes |
by |
T4017 |
8389-8392 |
NN |
denotes |
PCR |
T4018 |
8392-8393 |
. |
denotes |
. |
T4019 |
8393-8678 |
sentence |
denotes |
100 mm plates with these cells were further transfected by the calcium phosphate method (30) with 19.6 µg of an expression plasmid for Cre recombinase driven by the CMV promoter and 0.4 µg of an expression plasmid for the neomycin resistance gene under the control of the TK promoter. |
T4020 |
8394-8397 |
CD |
denotes |
100 |
T4021 |
8398-8400 |
NN |
denotes |
mm |
T4022 |
8401-8407 |
NNS |
denotes |
plates |
T4023 |
8438-8449 |
VBN |
denotes |
transfected |
T4024 |
8408-8412 |
IN |
denotes |
with |
T4025 |
8413-8418 |
DT |
denotes |
these |
T4026 |
8419-8424 |
NNS |
denotes |
cells |
T4027 |
8425-8429 |
VBD |
denotes |
were |
T4028 |
8430-8437 |
RB |
denotes |
further |
T4029 |
8450-8452 |
IN |
denotes |
by |
T4030 |
8453-8456 |
DT |
denotes |
the |
T4031 |
8475-8481 |
NN |
denotes |
method |
T4032 |
8457-8464 |
NN |
denotes |
calcium |
T4033 |
8465-8474 |
NN |
denotes |
phosphate |
T4034 |
8482-8483 |
-LRB- |
denotes |
( |
T4035 |
8483-8485 |
CD |
denotes |
30 |
T4036 |
8485-8486 |
-RRB- |
denotes |
) |
T4037 |
8487-8491 |
IN |
denotes |
with |
T4038 |
8492-8496 |
CD |
denotes |
19.6 |
T4039 |
8497-8499 |
NN |
denotes |
µg |
T4040 |
8500-8502 |
IN |
denotes |
of |
T4041 |
8503-8505 |
DT |
denotes |
an |
T4042 |
8517-8524 |
NN |
denotes |
plasmid |
T4043 |
8506-8516 |
NN |
denotes |
expression |
T4044 |
8525-8528 |
IN |
denotes |
for |
T4045 |
8529-8532 |
NN |
denotes |
Cre |
T4046 |
8533-8544 |
NN |
denotes |
recombinase |
T4047 |
8545-8551 |
VBN |
denotes |
driven |
T4048 |
8552-8554 |
IN |
denotes |
by |
T4049 |
8555-8558 |
DT |
denotes |
the |
T4050 |
8563-8571 |
NN |
denotes |
promoter |
T4051 |
8559-8562 |
NN |
denotes |
CMV |
T4052 |
8572-8575 |
CC |
denotes |
and |
T4053 |
8576-8579 |
CD |
denotes |
0.4 |
T4054 |
8580-8582 |
NN |
denotes |
µg |
T4055 |
8583-8585 |
IN |
denotes |
of |
T4056 |
8586-8588 |
DT |
denotes |
an |
T4057 |
8600-8607 |
NN |
denotes |
plasmid |
T4058 |
8589-8599 |
NN |
denotes |
expression |
T4059 |
8608-8611 |
IN |
denotes |
for |
T4060 |
8612-8615 |
DT |
denotes |
the |
T4061 |
8636-8640 |
NN |
denotes |
gene |
T4062 |
8616-8624 |
NN |
denotes |
neomycin |
T4063 |
8625-8635 |
NN |
denotes |
resistance |
T4064 |
8641-8646 |
IN |
denotes |
under |
T4065 |
8647-8650 |
DT |
denotes |
the |
T4066 |
8651-8658 |
NN |
denotes |
control |
T4067 |
8659-8661 |
IN |
denotes |
of |
T4068 |
8662-8665 |
DT |
denotes |
the |
T4069 |
8669-8677 |
NN |
denotes |
promoter |
T4070 |
8666-8668 |
NN |
denotes |
TK |
T4071 |
8677-8678 |
. |
denotes |
. |
T4072 |
8678-8941 |
sentence |
denotes |
Stably transfected cells were selected in the presence of 0.4 µg/µl G418 (Calbiochem), isolated clones of resistant cells were harvested and grown independently, and the expression of Cre recombinase and excision of exons 3 and 4 of Mtf1 were analyzed by RT–PCR. |
T4073 |
8679-8685 |
RB |
denotes |
Stably |
T4074 |
8686-8697 |
VBN |
denotes |
transfected |
T4075 |
8698-8703 |
NNS |
denotes |
cells |
T4076 |
8709-8717 |
VBN |
denotes |
selected |
T4077 |
8704-8708 |
VBD |
denotes |
were |
T4078 |
8718-8720 |
IN |
denotes |
in |
T4079 |
8721-8724 |
DT |
denotes |
the |
T4080 |
8725-8733 |
NN |
denotes |
presence |
T4081 |
8734-8736 |
IN |
denotes |
of |
T4082 |
8737-8740 |
CD |
denotes |
0.4 |
T4083 |
8741-8743 |
NN |
denotes |
µg |
T4084 |
8747-8751 |
NN |
denotes |
G418 |
T4085 |
8743-8744 |
SYM |
denotes |
/ |
T4086 |
8744-8746 |
NN |
denotes |
µl |
T4087 |
8752-8753 |
-LRB- |
denotes |
( |
T4088 |
8753-8763 |
NNP |
denotes |
Calbiochem |
T4089 |
8763-8764 |
-RRB- |
denotes |
) |
T4090 |
8764-8766 |
, |
denotes |
, |
T4091 |
8766-8774 |
VBN |
denotes |
isolated |
T4092 |
8775-8781 |
NNS |
denotes |
clones |
T4093 |
8806-8815 |
VBN |
denotes |
harvested |
T4094 |
8782-8784 |
IN |
denotes |
of |
T4095 |
8785-8794 |
JJ |
denotes |
resistant |
T4096 |
8795-8800 |
NNS |
denotes |
cells |
T4097 |
8801-8805 |
VBD |
denotes |
were |
T4098 |
8816-8819 |
CC |
denotes |
and |
T4099 |
8820-8825 |
VBN |
denotes |
grown |
T4100 |
8826-8839 |
RB |
denotes |
independently |
T4101 |
8839-8841 |
, |
denotes |
, |
T4102 |
8841-8844 |
CC |
denotes |
and |
T4103 |
8845-8848 |
DT |
denotes |
the |
T4104 |
8849-8859 |
NN |
denotes |
expression |
T4105 |
8922-8930 |
VBN |
denotes |
analyzed |
T4106 |
8860-8862 |
IN |
denotes |
of |
T4107 |
8863-8866 |
NN |
denotes |
Cre |
T4108 |
8867-8878 |
NN |
denotes |
recombinase |
T4109 |
8879-8882 |
CC |
denotes |
and |
T4110 |
8883-8891 |
NN |
denotes |
excision |
T4111 |
8892-8894 |
IN |
denotes |
of |
T4112 |
8895-8900 |
NNS |
denotes |
exons |
T4113 |
8901-8902 |
CD |
denotes |
3 |
T4114 |
8903-8906 |
CC |
denotes |
and |
T4115 |
8907-8908 |
CD |
denotes |
4 |
T4116 |
8909-8911 |
IN |
denotes |
of |
T4117 |
8912-8916 |
NN |
denotes |
Mtf1 |
T4118 |
8917-8921 |
VBD |
denotes |
were |
T4119 |
8931-8933 |
IN |
denotes |
by |
T4120 |
8934-8936 |
NN |
denotes |
RT |
T4121 |
8937-8940 |
NN |
denotes |
PCR |
T4122 |
8936-8937 |
HYPH |
denotes |
– |
T4123 |
8940-8941 |
. |
denotes |
. |
T4124 |
8941-9043 |
sentence |
denotes |
The cell lines delC19, delC21 and delC23 with a deletion of Mtf1 were chosen for further experiments. |
T4125 |
8942-8945 |
DT |
denotes |
The |
T4126 |
8951-8956 |
NNS |
denotes |
lines |
T4127 |
8946-8950 |
NN |
denotes |
cell |
T4128 |
9012-9018 |
VBN |
denotes |
chosen |
T4129 |
8957-8963 |
NN |
denotes |
delC19 |
T4130 |
8963-8965 |
, |
denotes |
, |
T4131 |
8965-8971 |
NN |
denotes |
delC21 |
T4132 |
8972-8975 |
CC |
denotes |
and |
T4133 |
8976-8982 |
NN |
denotes |
delC23 |
T4134 |
8983-8987 |
IN |
denotes |
with |
T4135 |
8988-8989 |
DT |
denotes |
a |
T4136 |
8990-8998 |
NN |
denotes |
deletion |
T4137 |
8999-9001 |
IN |
denotes |
of |
T4138 |
9002-9006 |
NN |
denotes |
Mtf1 |
T4139 |
9007-9011 |
VBD |
denotes |
were |
T4140 |
9019-9022 |
IN |
denotes |
for |
T4141 |
9023-9030 |
JJ |
denotes |
further |
T4142 |
9031-9042 |
NNS |
denotes |
experiments |
T4143 |
9042-9043 |
. |
denotes |
. |
T3137 |
4993-4995 |
NN |
denotes |
S1 |
T3138 |
5005-5012 |
NN |
denotes |
mapping |
T3139 |
4996-5004 |
NN |
denotes |
nuclease |
T3140 |
5013-5015 |
IN |
denotes |
of |
T3141 |
5016-5027 |
NNS |
denotes |
transcripts |
T3142 |
5028-5029 |
-LRB- |
denotes |
( |
T3143 |
5032-5040 |
NN |
denotes |
analysis |
T3144 |
5029-5031 |
NN |
denotes |
S1 |
T3145 |
5040-5041 |
-RRB- |
denotes |
) |
T3146 |
5041-5178 |
sentence |
denotes |
S1 analysis was performed as previously described (29), using 100 µg DNase I-digested total RNA (RNA isolation see microarray analysis). |
T3147 |
5042-5044 |
NN |
denotes |
S1 |
T3148 |
5045-5053 |
NN |
denotes |
analysis |
T3149 |
5058-5067 |
VBN |
denotes |
performed |
T3150 |
5054-5057 |
VBD |
denotes |
was |
T3151 |
5068-5070 |
IN |
denotes |
as |
T3152 |
5082-5091 |
VBN |
denotes |
described |
T3153 |
5071-5081 |
RB |
denotes |
previously |
T3154 |
5092-5093 |
-LRB- |
denotes |
( |
T3155 |
5093-5095 |
CD |
denotes |
29 |
T3156 |
5095-5096 |
-RRB- |
denotes |
) |
T3157 |
5096-5098 |
, |
denotes |
, |
T3158 |
5098-5103 |
VBG |
denotes |
using |
T3159 |
5104-5107 |
CD |
denotes |
100 |
T3160 |
5108-5110 |
NN |
denotes |
µg |
T3161 |
5134-5137 |
NN |
denotes |
RNA |
T3162 |
5111-5116 |
NN |
denotes |
DNase |
T3163 |
5119-5127 |
VBN |
denotes |
digested |
T3184 |
5225-5234 |
NNP |
denotes |
Molecular |
T3196 |
5286-5289 |
NN |
denotes |
32P |
T3164 |
5117-5118 |
CD |
denotes |
I |
T3165 |
5118-5119 |
HYPH |
denotes |
- |
T3166 |
5128-5133 |
JJ |
denotes |
total |
T3167 |
5138-5139 |
-LRB- |
denotes |
( |
T3168 |
5153-5156 |
VBP |
denotes |
see |
T3169 |
5139-5142 |
NN |
denotes |
RNA |
T3170 |
5143-5152 |
NN |
denotes |
isolation |
T3171 |
5157-5167 |
NN |
denotes |
microarray |
T3172 |
5168-5176 |
NN |
denotes |
analysis |
T3173 |
5176-5177 |
-RRB- |
denotes |
) |
T3174 |
5177-5178 |
. |
denotes |
. |
T3175 |
5178-5245 |
sentence |
denotes |
The gels were developed using PhosphorImager (Molecular Dynamics). |
T3176 |
5179-5182 |
DT |
denotes |
The |
T3177 |
5183-5187 |
NNS |
denotes |
gels |
T3178 |
5193-5202 |
VBN |
denotes |
developed |
T3179 |
5188-5192 |
VBD |
denotes |
were |
T3180 |
5203-5208 |
VBG |
denotes |
using |
T3181 |
5209-5223 |
NNP |
denotes |
PhosphorImager |
T3182 |
5224-5225 |
-LRB- |
denotes |
( |
T3183 |
5235-5243 |
NNP |
denotes |
Dynamics |
T3185 |
5243-5244 |
-RRB- |
denotes |
) |
T3186 |
5244-5245 |
. |
denotes |
. |
T3187 |
5245-5512 |
sentence |
denotes |
S1 analysis was done with the following 32P-labeled oligonucleotides: Hprt S1: 5′-TCTTCAGTCTGATAAAATCTACAGTCATAGGAATGGATCTATCACTATTTCTATTCAGTGATTACATTAAAG-3′, Sepw1 S1: 5′-TTCAACCGGGAACACCTGGAAACATGGCTGCCTGTCTTCTTGAAGTCTTGAGGTGGAAAGGGAAAGCAAAGCAGGAGGGTTTTCCCACCC-3′. |
T3188 |
5246-5248 |
NN |
denotes |
S1 |
T3189 |
5249-5257 |
NN |
denotes |
analysis |
T3190 |
5262-5266 |
VBN |
denotes |
done |
T3191 |
5258-5261 |
VBD |
denotes |
was |
T3192 |
5267-5271 |
IN |
denotes |
with |
T3193 |
5272-5275 |
DT |
denotes |
the |
T3194 |
5298-5314 |
NNS |
denotes |
oligonucleotides |
T3195 |
5276-5285 |
VBG |
denotes |
following |
T3197 |
5290-5297 |
VBN |
denotes |
labeled |
T3198 |
5289-5290 |
HYPH |
denotes |
- |
T3199 |
5314-5316 |
: |
denotes |
: |
T3200 |
5316-5320 |
NN |
denotes |
Hprt |
T3201 |
5321-5323 |
NN |
denotes |
S1 |
T3202 |
5323-5325 |
: |
denotes |
: |
T3203 |
5325-5326 |
CD |
denotes |
5 |
T3204 |
5328-5400 |
NN |
denotes |
TCTTCAGTCTGATAAAATCTACAGTCATAGGAATGGATCTATCACTATTTCTATTCAGTGATTACATTAAAG |
T3205 |
5326-5327 |
SYM |
denotes |
′ |
T3206 |
5327-5328 |
HYPH |
denotes |
- |
T3207 |
5400-5401 |
HYPH |
denotes |
- |
T3208 |
5401-5402 |
CD |
denotes |
3 |
T3209 |
5402-5403 |
SYM |
denotes |
′ |
T3210 |
5403-5405 |
, |
denotes |
, |
T3211 |
5405-5410 |
NN |
denotes |
Sepw1 |
T3212 |
5411-5413 |
NN |
denotes |
S1 |
T3213 |
5413-5415 |
: |
denotes |
: |
T3214 |
5415-5416 |
CD |
denotes |
5 |
T3215 |
5418-5508 |
NN |
denotes |
TTCAACCGGGAACACCTGGAAACATGGCTGCCTGTCTTCTTGAAGTCTTGAGGTGGAAAGGGAAAGCAAAGCAGGAGGGTTTTCCCACCC |
T3216 |
5416-5417 |
SYM |
denotes |
′ |
T3217 |
5417-5418 |
HYPH |
denotes |
- |
T3218 |
5508-5509 |
HYPH |
denotes |
- |
T3219 |
5509-5510 |
CD |
denotes |
3 |
T3220 |
5510-5511 |
SYM |
denotes |
′ |
T3221 |
5511-5512 |
. |
denotes |
. |
T4279 |
9045-9057 |
NN |
denotes |
Cytotoxicity |
T4280 |
9058-9063 |
NN |
denotes |
assay |
T4281 |
9063-9170 |
sentence |
denotes |
Samples of 1 × 104 cells/well were plated in 96-well tissue culture plates and allowed to adhere for 24 h. |
T4282 |
9064-9071 |
NNS |
denotes |
Samples |
T4283 |
9099-9105 |
VBN |
denotes |
plated |
T4284 |
9072-9074 |
IN |
denotes |
of |
T4285 |
9075-9076 |
CD |
denotes |
1 |
T4286 |
9079-9082 |
CD |
denotes |
104 |
T4287 |
9077-9078 |
SYM |
denotes |
× |
T4288 |
9083-9088 |
NNS |
denotes |
cells |
T4289 |
9088-9089 |
SYM |
denotes |
/ |
T4290 |
9089-9093 |
NN |
denotes |
well |
T4291 |
9094-9098 |
VBD |
denotes |
were |
T4292 |
9106-9108 |
IN |
denotes |
in |
T4293 |
9109-9111 |
CD |
denotes |
96 |
T4294 |
9112-9116 |
NN |
denotes |
well |
T4295 |
9111-9112 |
HYPH |
denotes |
- |
T4296 |
9132-9138 |
NNS |
denotes |
plates |
T4297 |
9117-9123 |
NN |
denotes |
tissue |
T4298 |
9124-9131 |
NN |
denotes |
culture |
T4299 |
9139-9142 |
CC |
denotes |
and |
T4300 |
9143-9150 |
VBN |
denotes |
allowed |
T4301 |
9151-9153 |
TO |
denotes |
to |
T4302 |
9154-9160 |
VB |
denotes |
adhere |
T4303 |
9161-9164 |
IN |
denotes |
for |
T4304 |
9165-9167 |
CD |
denotes |
24 |
T4305 |
9168-9169 |
NN |
denotes |
h |
T4306 |
9169-9170 |
. |
denotes |
. |
T4307 |
9170-9351 |
sentence |
denotes |
The cells were then pre-incubated for 24 h in medium containing 0, 5, 10, 25 or 50 µM l-buthionine-[S,R]-sulfoximine (BSO) (Sigma), a drug that inhibits glutathione synthesis (31). |
T4308 |
9171-9174 |
DT |
denotes |
The |
T4309 |
9175-9180 |
NNS |
denotes |
cells |
T4310 |
9191-9204 |
VBN |
denotes |
pre-incubated |
T4311 |
9181-9185 |
VBD |
denotes |
were |
T4312 |
9186-9190 |
RB |
denotes |
then |
T4313 |
9205-9208 |
IN |
denotes |
for |
T4314 |
9209-9211 |
CD |
denotes |
24 |
T4315 |
9212-9213 |
NN |
denotes |
h |
T4316 |
9214-9216 |
IN |
denotes |
in |
T4317 |
9217-9223 |
NN |
denotes |
medium |
T4318 |
9224-9234 |
VBG |
denotes |
containing |
T4319 |
9235-9236 |
CD |
denotes |
0 |
T4320 |
9254-9256 |
NN |
denotes |
µM |
T4321 |
9236-9238 |
, |
denotes |
, |
T4322 |
9238-9239 |
CD |
denotes |
5 |
T4323 |
9239-9241 |
, |
denotes |
, |
T4324 |
9241-9243 |
CD |
denotes |
10 |
T4325 |
9243-9245 |
, |
denotes |
, |
T4326 |
9245-9247 |
CD |
denotes |
25 |
T4327 |
9248-9250 |
CC |
denotes |
or |
T4328 |
9251-9253 |
CD |
denotes |
50 |
T4329 |
9257-9258 |
NN |
denotes |
l |
T4330 |
9276-9287 |
NN |
denotes |
sulfoximine |
T4331 |
9258-9259 |
HYPH |
denotes |
- |
T4332 |
9259-9269 |
NN |
denotes |
buthionine |
T4333 |
9269-9270 |
HYPH |
denotes |
- |
T4334 |
9270-9271 |
-LRB- |
denotes |
[ |
T4335 |
9271-9272 |
NN |
denotes |
S |
T4336 |
9272-9273 |
, |
denotes |
, |
T4337 |
9273-9274 |
NN |
denotes |
R |
T4338 |
9274-9275 |
-RRB- |
denotes |
] |
T4339 |
9275-9276 |
HYPH |
denotes |
- |
T4340 |
9288-9289 |
-LRB- |
denotes |
( |
T4341 |
9289-9292 |
NN |
denotes |
BSO |
T4342 |
9292-9293 |
-RRB- |
denotes |
) |
T4343 |
9294-9295 |
-LRB- |
denotes |
( |
T4344 |
9295-9300 |
NNP |
denotes |
Sigma |
T4345 |
9300-9301 |
-RRB- |
denotes |
) |
T4346 |
9301-9303 |
, |
denotes |
, |
T4347 |
9303-9304 |
DT |
denotes |
a |
T4348 |
9305-9309 |
NN |
denotes |
drug |
T4349 |
9310-9314 |
WDT |
denotes |
that |
T4350 |
9315-9323 |
VBZ |
denotes |
inhibits |
T4351 |
9324-9335 |
NN |
denotes |
glutathione |
T4352 |
9336-9345 |
NN |
denotes |
synthesis |
T4353 |
9346-9347 |
-LRB- |
denotes |
( |
T4354 |
9347-9349 |
CD |
denotes |
31 |
T4355 |
9349-9350 |
-RRB- |
denotes |
) |
T4356 |
9350-9351 |
. |
denotes |
. |
T4357 |
9351-9467 |
sentence |
denotes |
Later, cells were exposed to 0, 5, 10 or 20 µM CdCl2 in the specified pre-incubation medium for an additional 24 h. |
T4358 |
9352-9357 |
RB |
denotes |
Later |
T4359 |
9370-9377 |
VBN |
denotes |
exposed |
T4360 |
9357-9359 |
, |
denotes |
, |
T4361 |
9359-9364 |
NNS |
denotes |
cells |
T4362 |
9365-9369 |
VBD |
denotes |
were |
T4363 |
9378-9380 |
IN |
denotes |
to |
T4364 |
9381-9382 |
CD |
denotes |
0 |
T4365 |
9396-9398 |
NN |
denotes |
µM |
T4366 |
9382-9384 |
, |
denotes |
, |
T4367 |
9384-9385 |
CD |
denotes |
5 |
T4368 |
9385-9387 |
, |
denotes |
, |
T4369 |
9387-9389 |
CD |
denotes |
10 |
T4370 |
9390-9392 |
CC |
denotes |
or |
T4371 |
9393-9395 |
CD |
denotes |
20 |
T4372 |
9399-9404 |
NN |
denotes |
CdCl2 |
T4373 |
9405-9407 |
IN |
denotes |
in |
T4374 |
9408-9411 |
DT |
denotes |
the |
T4375 |
9437-9443 |
NN |
denotes |
medium |
T4376 |
9412-9421 |
VBN |
denotes |
specified |
T4377 |
9422-9436 |
NN |
denotes |
pre-incubation |
T4378 |
9444-9447 |
IN |
denotes |
for |
T4379 |
9448-9450 |
DT |
denotes |
an |
T4380 |
9465-9466 |
NN |
denotes |
h |
T4381 |
9451-9461 |
JJ |
denotes |
additional |
T4382 |
9462-9464 |
CD |
denotes |
24 |
T4383 |
9466-9467 |
. |
denotes |
. |
T2931 |
4558-4579 |
NN |
denotes |
TTCCGATGTGCCAAGTGTGGC |
T4384 |
9467-9654 |
sentence |
denotes |
Cytotoxicity was determined by the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromid)-based Cell Proliferation Kit I (Roche) according to the manufacturer's instructions. |
T4385 |
9468-9480 |
NN |
denotes |
Cytotoxicity |
T4386 |
9485-9495 |
VBN |
denotes |
determined |
T4387 |
9481-9484 |
VBD |
denotes |
was |
T4388 |
9496-9498 |
IN |
denotes |
by |
T4389 |
9499-9502 |
DT |
denotes |
the |
T4390 |
9595-9598 |
NNP |
denotes |
Kit |
T4391 |
9503-9506 |
NN |
denotes |
MTT |
T4392 |
9570-9575 |
VBN |
denotes |
based |
T4393 |
9507-9508 |
-LRB- |
denotes |
( |
T4394 |
9562-9568 |
NN |
denotes |
bromid |
T4395 |
9508-9509 |
CD |
denotes |
3 |
T4396 |
9515-9530 |
NN |
denotes |
dimethylthiazol |
T4397 |
9509-9510 |
HYPH |
denotes |
- |
T4398 |
9510-9511 |
-LRB- |
denotes |
[ |
T4399 |
9511-9512 |
CD |
denotes |
4 |
T4400 |
9512-9513 |
, |
denotes |
, |
T4401 |
9513-9514 |
CD |
denotes |
5 |
T4402 |
9514-9515 |
HYPH |
denotes |
- |
T4403 |
9530-9531 |
HYPH |
denotes |
- |
T4404 |
9531-9532 |
CD |
denotes |
2 |
T4405 |
9533-9535 |
NN |
denotes |
yl |
T4406 |
9532-9533 |
HYPH |
denotes |
- |
T4407 |
9535-9536 |
-RRB- |
denotes |
] |
T4408 |
9536-9537 |
HYPH |
denotes |
- |
T4409 |
9537-9538 |
CD |
denotes |
2 |
T4410 |
9538-9539 |
, |
denotes |
, |
T4411 |
9539-9540 |
CD |
denotes |
5 |
T4412 |
9541-9549 |
NN |
denotes |
diphenyl |
T4413 |
9540-9541 |
HYPH |
denotes |
- |
T4414 |
9550-9561 |
NN |
denotes |
tetrazolium |
T4415 |
9568-9569 |
-RRB- |
denotes |
) |
T4416 |
9569-9570 |
HYPH |
denotes |
- |
T4417 |
9576-9580 |
NNP |
denotes |
Cell |
T4418 |
9581-9594 |
NNP |
denotes |
Proliferation |
T4419 |
9599-9600 |
CD |
denotes |
I |
T4420 |
9601-9602 |
-LRB- |
denotes |
( |
T4421 |
9602-9607 |
NNP |
denotes |
Roche |
T4422 |
9607-9608 |
-RRB- |
denotes |
) |
T4423 |
9609-9618 |
VBG |
denotes |
according |
T4424 |
9619-9621 |
IN |
denotes |
to |
T4425 |
9622-9625 |
DT |
denotes |
the |
T4426 |
9626-9638 |
NN |
denotes |
manufacturer |
T4427 |
9641-9653 |
NNS |
denotes |
instructions |
T4428 |
9638-9640 |
POS |
denotes |
's |
T4429 |
9653-9654 |
. |
denotes |
. |
T2932 |
4282-4284 |
NN |
denotes |
RT |
T2933 |
4285-4288 |
NN |
denotes |
PCR |
T2934 |
4284-4285 |
HYPH |
denotes |
– |
T2935 |
4288-4487 |
sentence |
denotes |
All RT–PCRs were performed with QIAGEN® OneStep RT–PCR Kit (QIAGEN) according to the manufacturer's instructions, using 150–200 ng DNase I-digested total RNA (RNA isolation see microarray analysis). |
T2936 |
4289-4292 |
DT |
denotes |
All |
T2937 |
4296-4300 |
NNS |
denotes |
PCRs |
T2938 |
4293-4295 |
NN |
denotes |
RT |
T2939 |
4295-4296 |
HYPH |
denotes |
– |
T2940 |
4306-4315 |
VBN |
denotes |
performed |
T2941 |
4301-4305 |
VBD |
denotes |
were |
T2942 |
4316-4320 |
IN |
denotes |
with |
T2943 |
4321-4328 |
NNP |
denotes |
QIAGEN® |
T2944 |
4344-4347 |
NN |
denotes |
Kit |
T2945 |
4329-4336 |
NNP |
denotes |
OneStep |
T2946 |
4337-4339 |
NN |
denotes |
RT |
T2947 |
4340-4343 |
NN |
denotes |
PCR |
T2948 |
4339-4340 |
HYPH |
denotes |
– |
T2949 |
4348-4349 |
-LRB- |
denotes |
( |
T2950 |
4349-4355 |
NNP |
denotes |
QIAGEN |
T2951 |
4355-4356 |
-RRB- |
denotes |
) |
T2952 |
4357-4366 |
VBG |
denotes |
according |
T2953 |
4367-4369 |
IN |
denotes |
to |
T2954 |
4370-4373 |
DT |
denotes |
the |
T2955 |
4374-4386 |
NN |
denotes |
manufacturer |
T2956 |
4389-4401 |
NNS |
denotes |
instructions |
T2957 |
4386-4388 |
POS |
denotes |
's |
T2958 |
4401-4403 |
, |
denotes |
, |
T2959 |
4403-4408 |
VBG |
denotes |
using |
T2960 |
4409-4412 |
CD |
denotes |
150 |
T2961 |
4413-4416 |
CD |
denotes |
200 |
T2962 |
4412-4413 |
SYM |
denotes |
– |
T2963 |
4417-4419 |
NN |
denotes |
ng |
T2964 |
4443-4446 |
NN |
denotes |
RNA |
T2965 |
4420-4425 |
NN |
denotes |
DNase |
T2966 |
4426-4427 |
NN |
denotes |
I |
T2967 |
4428-4436 |
VBN |
denotes |
digested |
T2968 |
4427-4428 |
HYPH |
denotes |
- |
T2969 |
4437-4442 |
JJ |
denotes |
total |
T2970 |
4447-4448 |
-LRB- |
denotes |
( |
T2971 |
4452-4461 |
NN |
denotes |
isolation |
T2972 |
4448-4451 |
NN |
denotes |
RNA |
T2973 |
4462-4465 |
VBP |
denotes |
see |
T2974 |
4466-4476 |
NN |
denotes |
microarray |
T2975 |
4477-4485 |
NN |
denotes |
analysis |
T2976 |
4485-4486 |
-RRB- |
denotes |
) |
T2977 |
4486-4487 |
. |
denotes |
. |
T2978 |
4487-4991 |
sentence |
denotes |
The reactions were carried out using the following primers: Csrp1: 5′-TTCCGATGTGCCAAGTGTGGC-3′; 5′-AGTAGAGAGTGGACATTCAGC-3′, hypoxanthin-guanin-phosphoribosyltransferase (Hprt): 5′-GCTGGTGAAAAGGACCTCTCG-3′; 5′-CCACAGGACTAGGACACCTGC-3′, Mtf1: 5′-GTGACTTTTGAGACTGTACTGAGTG-3′; 5′-CATGCCAAGAAACATTGAAGGTG-3′, Ndrg1: 5′-AGATACACAACAACGTGGAGG-3′; 5′-TGTGCGAGCGGCTTCGGGGGC-3′, Sepw1: 5′-TAGAGGCAGGGTCCTGAAAGC-3′; 5′-ACACCTGGAAACATGGCTGCC-3′, Slc39a10: 5′-GCTGTGGCTGGTAGTAAAAGC-3′; 5′-GTGGCATGGGATGTAAACAGC-3′. |
T2979 |
4488-4491 |
DT |
denotes |
The |
T2980 |
4492-4501 |
NNS |
denotes |
reactions |
T2981 |
4507-4514 |
VBN |
denotes |
carried |
T2982 |
4502-4506 |
VBD |
denotes |
were |
T2983 |
4515-4518 |
RP |
denotes |
out |
T2984 |
4519-4524 |
VBG |
denotes |
using |
T2985 |
4525-4528 |
DT |
denotes |
the |
T2986 |
4539-4546 |
NNS |
denotes |
primers |
T2987 |
4529-4538 |
VBG |
denotes |
following |
T2988 |
4546-4548 |
: |
denotes |
: |
T2989 |
4548-4553 |
NN |
denotes |
Csrp1 |
T2990 |
4553-4555 |
: |
denotes |
: |
T2991 |
4555-4556 |
CD |
denotes |
5 |
T2992 |
4556-4557 |
SYM |
denotes |
′ |
T2993 |
4557-4558 |
HYPH |
denotes |
- |
T2994 |
4579-4580 |
HYPH |
denotes |
- |
T2995 |
4580-4581 |
CD |
denotes |
3 |
T2996 |
4581-4582 |
SYM |
denotes |
′ |
T2997 |
4582-4583 |
: |
denotes |
; |
T2998 |
4584-4585 |
CD |
denotes |
5 |
T2999 |
4587-4608 |
NN |
denotes |
AGTAGAGAGTGGACATTCAGC |
T3000 |
4585-4586 |
SYM |
denotes |
′ |
T3001 |
4586-4587 |
HYPH |
denotes |
- |
T3002 |
4608-4609 |
HYPH |
denotes |
- |
T3003 |
4609-4610 |
CD |
denotes |
3 |
T3004 |
4610-4611 |
SYM |
denotes |
′ |
T3005 |
4611-4613 |
, |
denotes |
, |
T3006 |
4613-4624 |
NN |
denotes |
hypoxanthin |
T3007 |
4632-4657 |
NN |
denotes |
phosphoribosyltransferase |
T3008 |
4624-4625 |
HYPH |
denotes |
- |
T3009 |
4625-4631 |
NN |
denotes |
guanin |
T3010 |
4631-4632 |
HYPH |
denotes |
- |
T3011 |
4658-4659 |
-LRB- |
denotes |
( |
T3012 |
4659-4663 |
NN |
denotes |
Hprt |
T3013 |
4663-4664 |
-RRB- |
denotes |
) |
T3014 |
4664-4666 |
: |
denotes |
: |
T3015 |
4666-4667 |
CD |
denotes |
5 |
T3016 |
4669-4690 |
NN |
denotes |
GCTGGTGAAAAGGACCTCTCG |
T3017 |
4667-4668 |
SYM |
denotes |
′ |
T3018 |
4668-4669 |
HYPH |
denotes |
- |
T3019 |
4690-4691 |
HYPH |
denotes |
- |
T3020 |
4691-4692 |
CD |
denotes |
3 |
T3021 |
4692-4693 |
SYM |
denotes |
′ |
T3022 |
4693-4694 |
: |
denotes |
; |
T3023 |
4695-4696 |
CD |
denotes |
5 |
T3024 |
4698-4719 |
NN |
denotes |
CCACAGGACTAGGACACCTGC |
T3027 |
4719-4720 |
HYPH |
denotes |
- |
T3028 |
4720-4721 |
CD |
denotes |
3 |
T3029 |
4721-4722 |
SYM |
denotes |
′ |
T3030 |
4722-4724 |
, |
denotes |
, |
T3031 |
4724-4728 |
NN |
denotes |
Mtf1 |
T3032 |
4728-4730 |
: |
denotes |
: |
T3033 |
4730-4731 |
CD |
denotes |
5 |
T3034 |
4733-4758 |
NN |
denotes |
GTGACTTTTGAGACTGTACTGAGTG |
T3035 |
4731-4732 |
SYM |
denotes |
′ |
T3036 |
4732-4733 |
HYPH |
denotes |
- |
T3037 |
4758-4759 |
HYPH |
denotes |
- |
T3038 |
4759-4760 |
CD |
denotes |
3 |
T3039 |
4760-4761 |
SYM |
denotes |
′ |
T3040 |
4761-4762 |
: |
denotes |
; |
T3041 |
4763-4764 |
CD |
denotes |
5 |
T3042 |
4766-4789 |
NN |
denotes |
CATGCCAAGAAACATTGAAGGTG |
T3043 |
4764-4765 |
SYM |
denotes |
′ |
T3044 |
4765-4766 |
HYPH |
denotes |
- |
T3045 |
4789-4790 |
HYPH |
denotes |
- |
T3046 |
4790-4791 |
CD |
denotes |
3 |
T3047 |
4791-4792 |
SYM |
denotes |
′ |
T3048 |
4792-4794 |
, |
denotes |
, |
T2279 |
1964-1968 |
IN |
denotes |
from |
T3049 |
4794-4799 |
NN |
denotes |
Ndrg1 |
T3050 |
4799-4801 |
: |
denotes |
: |
T3051 |
4801-4802 |
CD |
denotes |
5 |
T3052 |
4804-4825 |
NN |
denotes |
AGATACACAACAACGTGGAGG |
T3053 |
4802-4803 |
SYM |
denotes |
′ |
T2293 |
2031-2033 |
NN |
denotes |
pC |
T3054 |
4803-4804 |
HYPH |
denotes |
- |
T3055 |
4825-4826 |
HYPH |
denotes |
- |
T3056 |
4826-4827 |
CD |
denotes |
3 |
T3057 |
4827-4828 |
SYM |
denotes |
′ |
T3058 |
4828-4829 |
: |
denotes |
; |
T3059 |
4830-4831 |
CD |
denotes |
5 |
T3060 |
4833-4854 |
NN |
denotes |
TGTGCGAGCGGCTTCGGGGGC |
T3061 |
4831-4832 |
SYM |
denotes |
′ |
T3062 |
4832-4833 |
HYPH |
denotes |
- |
T3063 |
4854-4855 |
HYPH |
denotes |
- |
T3064 |
4855-4856 |
CD |
denotes |
3 |
T3065 |
4856-4857 |
SYM |
denotes |
′ |
T3066 |
4857-4859 |
, |
denotes |
, |
T3067 |
4859-4864 |
NN |
denotes |
Sepw1 |
T3068 |
4864-4866 |
: |
denotes |
: |
T3069 |
4866-4867 |
CD |
denotes |
5 |
T3070 |
4869-4890 |
NN |
denotes |
TAGAGGCAGGGTCCTGAAAGC |
T3071 |
4867-4868 |
SYM |
denotes |
′ |
T3072 |
4868-4869 |
HYPH |
denotes |
- |
T3073 |
4890-4891 |
HYPH |
denotes |
- |
T3074 |
4891-4892 |
CD |
denotes |
3 |
T3075 |
4892-4893 |
SYM |
denotes |
′ |
T3076 |
4893-4894 |
: |
denotes |
; |
T3077 |
4895-4896 |
CD |
denotes |
5 |
T3078 |
4898-4919 |
NN |
denotes |
ACACCTGGAAACATGGCTGCC |
T3079 |
4896-4897 |
SYM |
denotes |
′ |
T3080 |
4897-4898 |
HYPH |
denotes |
- |
T3081 |
4919-4920 |
HYPH |
denotes |
- |
T3082 |
4920-4921 |
CD |
denotes |
3 |
T3083 |
4921-4922 |
SYM |
denotes |
′ |
T3084 |
4922-4924 |
, |
denotes |
, |
T3085 |
4924-4932 |
NN |
denotes |
Slc39a10 |
T3086 |
4932-4934 |
: |
denotes |
: |
T3087 |
4934-4935 |
CD |
denotes |
5 |
T3088 |
4937-4958 |
NN |
denotes |
GCTGTGGCTGGTAGTAAAAGC |
T3089 |
4935-4936 |
SYM |
denotes |
′ |
T3090 |
4936-4937 |
HYPH |
denotes |
- |
T3091 |
4958-4959 |
HYPH |
denotes |
- |
T3092 |
4959-4960 |
CD |
denotes |
3 |
T3093 |
4960-4961 |
SYM |
denotes |
′ |
T3094 |
4961-4962 |
: |
denotes |
; |
T3095 |
4963-4964 |
CD |
denotes |
5 |
T3096 |
4966-4987 |
NN |
denotes |
GTGGCATGGGATGTAAACAGC |
T3097 |
4964-4965 |
SYM |
denotes |
′ |
T3098 |
4965-4966 |
HYPH |
denotes |
- |
T3099 |
4987-4988 |
HYPH |
denotes |
- |
T3100 |
4988-4989 |
CD |
denotes |
3 |
T3101 |
4989-4990 |
SYM |
denotes |
′ |
T3102 |
4990-4991 |
. |
denotes |
. |
T2173 |
1490-1496 |
NN |
denotes |
Animal |
T2174 |
1497-1506 |
NN |
denotes |
treatment |
T2175 |
1506-1904 |
sentence |
denotes |
At 8 weeks of age, male Mtf1loxP/loxP mice harboring the Mx-cre transgene (Mtf1loxP/loxP Mx-cre, abbr.: Mtf1Mx-cre) and control littermates without transgene (Mtf1loxP/loxP, abbr.: Mtf1loxP) received four intraperitoneal injections each of 300 µg synthetic double-stranded RNA polyinosinic–polycytidylic acid [pI–pC; Sigma; in a volume of 60 µl phosphate-buffered saline (PBS)] at 3 day intervals. |
T2176 |
1507-1509 |
IN |
denotes |
At |
T2177 |
1698-1706 |
VBD |
denotes |
received |
T2178 |
1510-1511 |
CD |
denotes |
8 |
T2179 |
1512-1517 |
NNS |
denotes |
weeks |
T2180 |
1518-1520 |
IN |
denotes |
of |
T2181 |
1521-1524 |
NN |
denotes |
age |
T2182 |
1524-1526 |
, |
denotes |
, |
T2183 |
1526-1530 |
JJ |
denotes |
male |
T2184 |
1545-1549 |
NNS |
denotes |
mice |
T2185 |
1531-1539 |
NN |
denotes |
Mtf1loxP |
T2186 |
1540-1544 |
NN |
denotes |
loxP |
T2187 |
1539-1540 |
HYPH |
denotes |
/ |
T2188 |
1550-1559 |
VBG |
denotes |
harboring |
T2189 |
1560-1563 |
DT |
denotes |
the |
T2190 |
1571-1580 |
NN |
denotes |
transgene |
T2191 |
1564-1566 |
NN |
denotes |
Mx |
T2192 |
1567-1570 |
NN |
denotes |
cre |
T2193 |
1566-1567 |
HYPH |
denotes |
- |
T2194 |
1581-1582 |
-LRB- |
denotes |
( |
T2195 |
1582-1590 |
NN |
denotes |
Mtf1loxP |
T2196 |
1599-1602 |
NN |
denotes |
cre |
T2197 |
1590-1591 |
HYPH |
denotes |
/ |
T2198 |
1591-1595 |
NN |
denotes |
loxP |
T2199 |
1596-1598 |
NN |
denotes |
Mx |
T2200 |
1598-1599 |
HYPH |
denotes |
- |
T2201 |
1602-1604 |
, |
denotes |
, |
T2202 |
1604-1609 |
RB |
denotes |
abbr. |
T2203 |
1618-1621 |
NN |
denotes |
cre |
T2204 |
1609-1611 |
: |
denotes |
: |
T2205 |
1611-1617 |
NN |
denotes |
Mtf1Mx |
T2206 |
1617-1618 |
HYPH |
denotes |
- |
T2207 |
1621-1622 |
-RRB- |
denotes |
) |
T2208 |
1623-1626 |
CC |
denotes |
and |
T2209 |
1627-1634 |
NN |
denotes |
control |
T2210 |
1635-1646 |
NNS |
denotes |
littermates |
T2211 |
1647-1654 |
IN |
denotes |
without |
T2212 |
1655-1664 |
NN |
denotes |
transgene |
T2213 |
1665-1666 |
-LRB- |
denotes |
( |
T2214 |
1666-1674 |
NN |
denotes |
Mtf1loxP |
T2215 |
1675-1679 |
NN |
denotes |
loxP |
T2216 |
1674-1675 |
HYPH |
denotes |
/ |
T2217 |
1679-1681 |
, |
denotes |
, |
T2218 |
1681-1686 |
NN |
denotes |
abbr. |
T2219 |
1688-1696 |
NN |
denotes |
Mtf1loxP |
T2220 |
1686-1688 |
: |
denotes |
: |
T2221 |
1696-1697 |
-RRB- |
denotes |
) |
T2222 |
1707-1711 |
CD |
denotes |
four |
T2223 |
1728-1738 |
NNS |
denotes |
injections |
T2224 |
1712-1727 |
JJ |
denotes |
intraperitoneal |
T2225 |
1739-1743 |
RB |
denotes |
each |
T2226 |
1744-1746 |
IN |
denotes |
of |
T2227 |
1747-1750 |
CD |
denotes |
300 |
T2228 |
1751-1753 |
NN |
denotes |
µg |
T2229 |
1811-1815 |
NN |
denotes |
acid |
T2230 |
1754-1763 |
JJ |
denotes |
synthetic |
T2231 |
1764-1770 |
JJ |
denotes |
double |
T2232 |
1771-1779 |
VBN |
denotes |
stranded |
T2233 |
2178-2180 |
CD |
denotes |
20 |
T2234 |
1770-1771 |
HYPH |
denotes |
- |
T2235 |
1780-1783 |
NN |
denotes |
RNA |
T2236 |
1784-1796 |
JJ |
denotes |
polyinosinic |
T2237 |
1797-1810 |
JJ |
denotes |
polycytidylic |
T3873 |
7730-7738 |
NN |
denotes |
Mtf1loxP |
T2238 |
1796-1797 |
HYPH |
denotes |
– |
T2239 |
1816-1817 |
-LRB- |
denotes |
[ |
T2240 |
1824-1829 |
NNP |
denotes |
Sigma |
T2241 |
1817-1819 |
NN |
denotes |
pI |
T3874 |
7838-7850 |
NN |
denotes |
streptomycin |
T2242 |
1820-1822 |
NN |
denotes |
pC |
T2243 |
1819-1820 |
HYPH |
denotes |
– |
T2244 |
1822-1823 |
: |
denotes |
; |
T2245 |
1829-1830 |
: |
denotes |
; |
T2246 |
1831-1833 |
IN |
denotes |
in |
T2247 |
1834-1835 |
DT |
denotes |
a |
T2248 |
1836-1842 |
NN |
denotes |
volume |
T2249 |
1843-1845 |
IN |
denotes |
of |
T2250 |
1846-1848 |
CD |
denotes |
60 |
T2251 |
1849-1851 |
NN |
denotes |
µl |
T2252 |
1852-1861 |
NN |
denotes |
phosphate |
T2253 |
1862-1870 |
VBN |
denotes |
buffered |
T2254 |
1861-1862 |
HYPH |
denotes |
- |
T2255 |
1871-1877 |
NN |
denotes |
saline |
T2256 |
1878-1879 |
-LRB- |
denotes |
( |
T2257 |
1879-1882 |
NN |
denotes |
PBS |
T2258 |
1882-1883 |
-RRB- |
denotes |
) |
T2259 |
1883-1884 |
-RRB- |
denotes |
] |
T2260 |
1885-1887 |
IN |
denotes |
at |
T2261 |
1888-1889 |
CD |
denotes |
3 |
T2262 |
1890-1893 |
NN |
denotes |
day |
T2263 |
1894-1903 |
NNS |
denotes |
intervals |
T2264 |
1903-1904 |
. |
denotes |
. |
T2265 |
1904-2045 |
sentence |
denotes |
Only in the case of DNA-binding studies with MRE sequences from MTF-1 target gene candidates, the control mice received no pI–pC injections. |
T2266 |
1905-1909 |
RB |
denotes |
Only |
T2267 |
1910-1912 |
IN |
denotes |
in |
T2268 |
2016-2024 |
VBD |
denotes |
received |
T2269 |
1913-1916 |
DT |
denotes |
the |
T2270 |
1917-1921 |
NN |
denotes |
case |
T2271 |
1922-1924 |
IN |
denotes |
of |
T2272 |
1925-1928 |
NN |
denotes |
DNA |
T2273 |
1929-1936 |
VBG |
denotes |
binding |
T2274 |
1928-1929 |
HYPH |
denotes |
- |
T2275 |
1937-1944 |
NNS |
denotes |
studies |
T2276 |
1945-1949 |
IN |
denotes |
with |
T2277 |
1950-1953 |
NN |
denotes |
MRE |
T2278 |
1954-1963 |
NNS |
denotes |
sequences |
T2280 |
1969-1972 |
NN |
denotes |
MTF |
T2281 |
1987-1997 |
NNS |
denotes |
candidates |
T2282 |
1972-1973 |
HYPH |
denotes |
- |
T2283 |
1973-1974 |
CD |
denotes |
1 |
T2284 |
1975-1981 |
NN |
denotes |
target |
T2285 |
1982-1986 |
NN |
denotes |
gene |
T2286 |
1997-1999 |
, |
denotes |
, |
T2287 |
1999-2002 |
DT |
denotes |
the |
T2288 |
2011-2015 |
NNS |
denotes |
mice |
T2289 |
2003-2010 |
NN |
denotes |
control |
T2290 |
2025-2027 |
DT |
denotes |
no |
T2291 |
2034-2044 |
NNS |
denotes |
injections |
T2292 |
2028-2030 |
NN |
denotes |
pI |
T2294 |
2030-2031 |
HYPH |
denotes |
– |
T2295 |
2044-2045 |
. |
denotes |
. |
T2296 |
2045-2319 |
sentence |
denotes |
For experiments with metal treatment, mice received 2 days after the last pI–pC treatment a subcutaneous (s.c.) injection of either 20 µmol/kg body weight CdSO4 (2 mM CdSO4 in H2O; cadmium treatment) or 10 ml/kg body weight H2O (mock treatment) 6 h before sacrificing them. |
T2297 |
2046-2049 |
IN |
denotes |
For |
T2298 |
2089-2097 |
VBD |
denotes |
received |
T2299 |
2050-2061 |
NNS |
denotes |
experiments |
T2300 |
2062-2066 |
IN |
denotes |
with |
T2301 |
2067-2072 |
NN |
denotes |
metal |
T2302 |
2073-2082 |
NN |
denotes |
treatment |
T2303 |
2082-2084 |
, |
denotes |
, |
T2304 |
2084-2088 |
NNS |
denotes |
mice |
T2305 |
2098-2099 |
CD |
denotes |
2 |
T2306 |
2100-2104 |
NNS |
denotes |
days |
T2307 |
2105-2110 |
IN |
denotes |
after |
T2308 |
2111-2114 |
DT |
denotes |
the |
T2309 |
2126-2135 |
NN |
denotes |
treatment |
T2310 |
2115-2119 |
JJ |
denotes |
last |
T2311 |
2120-2122 |
NN |
denotes |
pI |
T2312 |
2123-2125 |
NN |
denotes |
pC |
T2313 |
2122-2123 |
HYPH |
denotes |
– |
T2314 |
2136-2137 |
DT |
denotes |
a |
T2315 |
2158-2167 |
NN |
denotes |
injection |
T2316 |
2138-2150 |
JJ |
denotes |
subcutaneous |
T2317 |
2152-2156 |
JJ |
denotes |
s.c. |
T2318 |
2151-2152 |
-LRB- |
denotes |
( |
T2319 |
2156-2157 |
-RRB- |
denotes |
) |
T2320 |
2168-2170 |
IN |
denotes |
of |
T2321 |
2171-2177 |
CC |
denotes |
either |
T2322 |
2201-2206 |
NN |
denotes |
CdSO4 |
T2323 |
2181-2185 |
NN |
denotes |
µmol |
T2324 |
2185-2186 |
SYM |
denotes |
/ |
T2325 |
2194-2200 |
NN |
denotes |
weight |
T2326 |
2186-2188 |
NN |
denotes |
kg |
T2327 |
2189-2193 |
NN |
denotes |
body |
T2328 |
2207-2208 |
-LRB- |
denotes |
( |
T2329 |
2235-2244 |
NN |
denotes |
treatment |
T2330 |
2208-2209 |
CD |
denotes |
2 |
T2331 |
2210-2212 |
NN |
denotes |
mM |
T2334 |
2222-2225 |
NN |
denotes |
H2O |
T2332 |
2213-2218 |
NN |
denotes |
CdSO4 |
T2333 |
2219-2221 |
IN |
denotes |
in |
T2335 |
2225-2226 |
: |
denotes |
; |
T2336 |
2227-2234 |
NN |
denotes |
cadmium |
T2337 |
2244-2245 |
-RRB- |
denotes |
) |
T2338 |
2246-2248 |
CC |
denotes |
or |
T2339 |
2249-2251 |
CD |
denotes |
10 |
T2340 |
2252-2254 |
NN |
denotes |
ml |
T2341 |
2270-2273 |
NN |
denotes |
H2O |
T2342 |
2254-2255 |
SYM |
denotes |
/ |
T2343 |
2263-2269 |
NN |
denotes |
weight |
T2344 |
2255-2257 |
NN |
denotes |
kg |
T2345 |
2258-2262 |
NN |
denotes |
body |
T2346 |
2274-2275 |
-LRB- |
denotes |
( |
T2347 |
2280-2289 |
NN |
denotes |
treatment |
T2348 |
2275-2279 |
JJ |
denotes |
mock |
T2349 |
2289-2290 |
-RRB- |
denotes |
) |
T2350 |
2291-2292 |
CD |
denotes |
6 |
T2351 |
2293-2294 |
NN |
denotes |
h |
T2352 |
2295-2301 |
IN |
denotes |
before |
T2353 |
2302-2313 |
VBG |
denotes |
sacrificing |
T2354 |
2314-2318 |
PRP |
denotes |
them |
T2355 |
2318-2319 |
. |
denotes |
. |
T3875 |
7653-7657 |
NN |
denotes |
Cell |
T3876 |
7658-7665 |
NN |
denotes |
culture |
T3877 |
7665-7896 |
sentence |
denotes |
Primary embryonic fibroblasts were isolated from a 12.5 day old Mtf1loxP mouse embryo and grown in DMEM supplemented with 10% fetal bovine serum (ICN), 100 U/ml penicillin–streptomycin (Gibco BRL) and 2 mM l-glutamine (Gibco BRL). |
T3878 |
7666-7673 |
JJ |
denotes |
Primary |
T3879 |
7684-7695 |
NNS |
denotes |
fibroblasts |
T3880 |
7674-7683 |
JJ |
denotes |
embryonic |
T3881 |
7701-7709 |
VBN |
denotes |
isolated |
T3882 |
7696-7700 |
VBD |
denotes |
were |
T3883 |
7710-7714 |
IN |
denotes |
from |
T3884 |
7715-7716 |
DT |
denotes |
a |
T3885 |
7722-7725 |
NN |
denotes |
day |
T3886 |
7717-7721 |
CD |
denotes |
12.5 |
T3887 |
7726-7729 |
JJ |
denotes |
old |
T3888 |
7745-7751 |
NN |
denotes |
embryo |
T3889 |
7739-7744 |
NN |
denotes |
mouse |
T3890 |
7752-7755 |
CC |
denotes |
and |
T3891 |
7756-7761 |
VBN |
denotes |
grown |
T3892 |
7762-7764 |
IN |
denotes |
in |
T3893 |
7765-7769 |
NN |
denotes |
DMEM |
T3894 |
7770-7782 |
VBN |
denotes |
supplemented |
T3895 |
7783-7787 |
IN |
denotes |
with |
T3896 |
7788-7790 |
CD |
denotes |
10 |
T3897 |
7790-7791 |
NN |
denotes |
% |
T3898 |
7805-7810 |
NN |
denotes |
serum |
T3899 |
7792-7797 |
JJ |
denotes |
fetal |
T3900 |
7798-7804 |
JJ |
denotes |
bovine |
T3901 |
7811-7812 |
-LRB- |
denotes |
( |
T3902 |
7812-7815 |
NN |
denotes |
ICN |
T3903 |
7815-7816 |
-RRB- |
denotes |
) |
T3904 |
7816-7818 |
, |
denotes |
, |
T3905 |
7818-7821 |
CD |
denotes |
100 |
T3906 |
7822-7823 |
NN |
denotes |
U |
T3907 |
7823-7824 |
SYM |
denotes |
/ |
T3908 |
7824-7826 |
NN |
denotes |
ml |
T3909 |
7827-7837 |
NN |
denotes |
penicillin |
T3910 |
7837-7838 |
HYPH |
denotes |
– |
T3911 |
7851-7852 |
-LRB- |
denotes |
( |
T3912 |
7858-7861 |
NNP |
denotes |
BRL |
T3913 |
7852-7857 |
NNP |
denotes |
Gibco |
T3914 |
7861-7862 |
-RRB- |
denotes |
) |
T3915 |
7863-7866 |
CC |
denotes |
and |
T3916 |
7867-7868 |
CD |
denotes |
2 |
T3917 |
7869-7871 |
NN |
denotes |
mM |
T3918 |
7874-7883 |
NN |
denotes |
glutamine |
T3919 |
7872-7873 |
NN |
denotes |
l |
T3920 |
7873-7874 |
HYPH |
denotes |
- |
T3921 |
7884-7885 |
-LRB- |
denotes |
( |
T3922 |
7891-7894 |
NNP |
denotes |
BRL |
T3923 |
7885-7890 |
NNP |
denotes |
Gibco |
T3924 |
7894-7895 |
-RRB- |
denotes |
) |
T3925 |
7895-7896 |
. |
denotes |
. |
T3926 |
7896-8166 |
sentence |
denotes |
100 mm plates with primary cells were transfected with 10 µg of an expression plasmid coding for simian virus 40 (SV40) large T antigen driven by the cytomegalovirus (CMV) promoter, using lipofectamine™ reagent (Invitrogen) according to the manufacturer's instructions. |
T3927 |
7897-7900 |
CD |
denotes |
100 |
T3928 |
7901-7903 |
NN |
denotes |
mm |
T3929 |
7904-7910 |
NNS |
denotes |
plates |
T3930 |
7935-7946 |
VBN |
denotes |
transfected |
T3931 |
7911-7915 |
IN |
denotes |
with |
T3932 |
7916-7923 |
JJ |
denotes |
primary |
T3933 |
7924-7929 |
NNS |
denotes |
cells |
T3934 |
7930-7934 |
VBD |
denotes |
were |
T3935 |
7947-7951 |
IN |
denotes |
with |
T3936 |
7952-7954 |
CD |
denotes |
10 |
T3937 |
7955-7957 |
NN |
denotes |
µg |
T3938 |
7958-7960 |
IN |
denotes |
of |
T3939 |
7961-7963 |
DT |
denotes |
an |
T3940 |
7975-7982 |
NN |
denotes |
plasmid |
T3941 |
7964-7974 |
NN |
denotes |
expression |
T3942 |
7983-7989 |
VBG |
denotes |
coding |
T3943 |
7990-7993 |
IN |
denotes |
for |
T3944 |
7994-8000 |
JJ |
denotes |
simian |
T3945 |
8001-8006 |
NN |
denotes |
virus |
T3946 |
8025-8032 |
NN |
denotes |
antigen |
T3947 |
8007-8009 |
CD |
denotes |
40 |
T1954 |
1025-1234 |
sentence |
denotes |
Homozygous conditional knockout animals (Mtf1loxP/loxP) were crossed with the Cre recombinase transgenic line Mx-cre (a gift from Prof. Michel Aguet) to obtain an inducible, liver-specific Mtf1 knockout line. |
T2075 |
1108-1119 |
NN |
denotes |
recombinase |
T2076 |
1120-1130 |
JJ |
denotes |
transgenic |
T2077 |
1136-1138 |
NN |
denotes |
Mx |
T2078 |
1139-1142 |
NN |
denotes |
cre |
T2079 |
1138-1139 |
HYPH |
denotes |
- |
T2080 |
1143-1144 |
-LRB- |
denotes |
( |
T2081 |
1146-1150 |
NN |
denotes |
gift |
T2082 |
1144-1145 |
DT |
denotes |
a |
T2083 |
1151-1155 |
IN |
denotes |
from |
T2084 |
1156-1161 |
NNP |
denotes |
Prof. |
T2085 |
1169-1174 |
NNP |
denotes |
Aguet |
T2086 |
1162-1168 |
NNP |
denotes |
Michel |
T2087 |
1174-1175 |
-RRB- |
denotes |
) |
T2088 |
1176-1178 |
TO |
denotes |
to |
T2089 |
1179-1185 |
VB |
denotes |
obtain |
T2090 |
1186-1188 |
DT |
denotes |
an |
T2091 |
1229-1233 |
NN |
denotes |
line |
T2092 |
1189-1198 |
JJ |
denotes |
inducible |
T2093 |
1198-1200 |
, |
denotes |
, |
T2094 |
1200-1205 |
NN |
denotes |
liver |
T2095 |
1206-1214 |
JJ |
denotes |
specific |
T2096 |
1205-1206 |
HYPH |
denotes |
- |
T2097 |
1215-1219 |
NN |
denotes |
Mtf1 |
T2098 |
1220-1228 |
NN |
denotes |
knockout |
T2074 |
1104-1107 |
NN |
denotes |
Cre |
R1032 |
T1759 |
T1758 |
prep |
of,Generation |
R1033 |
T1760 |
T1761 |
nmod |
Mtf1,mice |
R1034 |
T1761 |
T1759 |
pobj |
mice,of |
R1035 |
T1762 |
T1763 |
amod |
conditional,knockout |
R1036 |
T1763 |
T1761 |
compound |
knockout,mice |
R1037 |
T1764 |
T1761 |
cc |
and,mice |
R1038 |
T1765 |
T1766 |
npadvmod |
liver,specific |
R1039 |
T1766 |
T1768 |
amod |
specific,deletion |
R1040 |
T1767 |
T1766 |
punct |
-,specific |
R1041 |
T1768 |
T1761 |
conj |
deletion,mice |
R1042 |
T1770 |
T1771 |
nmod |
Mtf1,mice |
R1043 |
T1771 |
T1774 |
nsubjpass |
mice,generated |
R1044 |
T1772 |
T1773 |
amod |
conditional,knockout |
R1045 |
T1773 |
T1771 |
compound |
knockout,mice |
R1046 |
T1775 |
T1774 |
auxpass |
were,generated |
R1047 |
T1776 |
T1774 |
prep |
in,generated |
R1048 |
T1777 |
T1776 |
pobj |
collaboration,in |
R1049 |
T1778 |
T1777 |
prep |
with,collaboration |
R1050 |
T1779 |
T1780 |
compound |
Dr,Leviten |
R1051 |
T1780 |
T1778 |
pobj |
Leviten,with |
R1052 |
T1781 |
T1780 |
compound |
Michael,Leviten |
R1053 |
T1782 |
T1783 |
punct |
(,Carlos |
R1054 |
T1783 |
T1780 |
parataxis |
Carlos,Leviten |
R1055 |
T1784 |
T1783 |
compound |
San,Carlos |
R1056 |
T1785 |
T1783 |
punct |
", ",Carlos |
R1057 |
T1786 |
T1783 |
npadvmod |
CA,Carlos |
R1058 |
T1787 |
T1783 |
punct |
),Carlos |
R1059 |
T1788 |
T1774 |
punct |
.,generated |
R1060 |
T1790 |
T1791 |
nummod |
Two,clones |
R1061 |
T1791 |
T1793 |
nsubjpass |
clones,used |
R1062 |
T1792 |
T1791 |
amod |
genomic,clones |
R1063 |
T1794 |
T1791 |
acl |
containing,clones |
R1064 |
T1795 |
T1796 |
nmod |
exons,3 |
R1065 |
T1796 |
T1794 |
dobj |
3,containing |
R1066 |
T1797 |
T1796 |
prep |
to,3 |
R1067 |
T1798 |
T1797 |
pobj |
6,to |
R1068 |
T1799 |
T1796 |
prep |
of,3 |
R1069 |
T1800 |
T1799 |
pobj |
Mtf1,of |
R1070 |
T1801 |
T1793 |
auxpass |
were,used |
R1071 |
T1802 |
T1803 |
aux |
to,construct |
R1072 |
T1803 |
T1793 |
advcl |
construct,used |
R1073 |
T1804 |
T1805 |
det |
a,vector |
R1074 |
T1805 |
T1803 |
dobj |
vector,construct |
R1075 |
T1806 |
T1807 |
compound |
gene,targeting |
R1076 |
T1807 |
T1805 |
compound |
targeting,vector |
R1077 |
T1808 |
T1803 |
prep |
for,construct |
R1078 |
T1809 |
T1810 |
amod |
homologous,recombination |
R1079 |
T1810 |
T1808 |
pobj |
recombination,for |
R1080 |
T1811 |
T1812 |
punct |
(,Data |
R1081 |
T1812 |
T1803 |
parataxis |
Data,construct |
R1082 |
T1813 |
T1812 |
amod |
Supplementary,Data |
R1083 |
T1814 |
T1812 |
punct |
),Data |
R1084 |
T1815 |
T1793 |
punct |
.,used |
R1085 |
T1817 |
T1818 |
det |
A,cassette |
R1086 |
T1818 |
T1821 |
nsubjpass |
cassette,cloned |
R1087 |
T1819 |
T1818 |
compound |
neomycin,cassette |
R1088 |
T1820 |
T1818 |
compound |
resistance,cassette |
R1089 |
T1821 |
T1833 |
ccomp |
cloned,cloned |
R1090 |
T1822 |
T1818 |
punct |
(,cassette |
R1091 |
T1823 |
T1824 |
compound |
PGK,neo |
R1092 |
T1824 |
T1818 |
appos |
neo,cassette |
R1093 |
T1825 |
T1824 |
punct |
-,neo |
R1094 |
T1826 |
T1818 |
punct |
),cassette |
R1095 |
T1827 |
T1818 |
acl |
flanked,cassette |
R1096 |
T1828 |
T1827 |
agent |
by,flanked |
R1097 |
T1829 |
T1830 |
nummod |
two,sites |
R1098 |
T1830 |
T1828 |
pobj |
sites,by |
R1099 |
T1831 |
T1830 |
compound |
loxP,sites |
R1100 |
T1832 |
T1821 |
auxpass |
was,cloned |
R1101 |
T1834 |
T1821 |
prep |
into,cloned |
R1102 |
T1835 |
T1836 |
det |
the,site |
R1103 |
T1836 |
T1834 |
pobj |
site,into |
R1104 |
T1837 |
T1836 |
compound |
SacI,site |
R1105 |
T1838 |
T1836 |
npadvmod |
5,site |
R1106 |
T1839 |
T1838 |
punct |
′,5 |
R1107 |
T1840 |
T1838 |
prep |
of,5 |
R1108 |
T1841 |
T1840 |
pobj |
exon,of |
R1109 |
T1842 |
T1841 |
nummod |
3,exon |
R1110 |
T1843 |
T1841 |
prep |
of,exon |
R1111 |
T1844 |
T1843 |
pobj |
Mtf1,of |
R1112 |
T1845 |
T1833 |
punct |
", ",cloned |
R1113 |
T1846 |
T1847 |
det |
the,site |
R1114 |
T1847 |
T1833 |
nsubjpass |
site,cloned |
R1115 |
T1848 |
T1847 |
amod |
third,site |
R1116 |
T1849 |
T1847 |
compound |
loxP,site |
R1117 |
T1850 |
T1833 |
auxpass |
was,cloned |
R1118 |
T1851 |
T1833 |
prep |
into,cloned |
R1119 |
T1852 |
T1853 |
det |
the,site |
R1120 |
T1853 |
T1851 |
pobj |
site,into |
R1121 |
T1854 |
T1853 |
compound |
ScaI,site |
R1122 |
T1855 |
T1853 |
npadvmod |
3,site |
R1123 |
T1856 |
T1855 |
punct |
′,3 |
R1124 |
T1857 |
T1855 |
prep |
of,3 |
R1125 |
T1858 |
T1857 |
pobj |
exon,of |
R1126 |
T1859 |
T1858 |
nummod |
4,exon |
R1127 |
T1860 |
T1833 |
punct |
.,cloned |
R1128 |
T1862 |
T1863 |
det |
A,cassette |
R1129 |
T1863 |
T1869 |
nsubjpass |
cassette,inserted |
R1130 |
T1864 |
T1865 |
nmod |
thymidine,kinase |
R1131 |
T1865 |
T1863 |
nmod |
kinase,cassette |
R1132 |
T1866 |
T1865 |
punct |
(,kinase |
R1133 |
T1867 |
T1865 |
appos |
TK,kinase |
R1134 |
T1868 |
T1863 |
punct |
),cassette |
R1135 |
T1870 |
T1869 |
auxpass |
was,inserted |
R1136 |
T1871 |
T1869 |
prep |
in,inserted |
R1137 |
T1872 |
T1873 |
det |
the,site |
R1138 |
T1873 |
T1871 |
pobj |
site,in |
R1139 |
T1874 |
T1873 |
compound |
HpaI,site |
R1140 |
T1875 |
T1873 |
npadvmod |
3,site |
R1141 |
T1876 |
T1875 |
punct |
′,3 |
R1142 |
T1877 |
T1875 |
prep |
of,3 |
R1143 |
T1878 |
T1877 |
pobj |
exon,of |
R1144 |
T1879 |
T1878 |
nummod |
6,exon |
R1145 |
T1880 |
T1869 |
punct |
.,inserted |
R1146 |
T1882 |
T1883 |
nummod |
129,cells |
R1147 |
T1883 |
T1885 |
nsubjpass |
cells,electroporated |
R1148 |
T1884 |
T1883 |
compound |
ES,cells |
R1149 |
T1886 |
T1885 |
auxpass |
were,electroporated |
R1150 |
T1887 |
T1885 |
prep |
with,electroporated |
R1151 |
T1888 |
T1889 |
det |
the,vector |
R1152 |
T1889 |
T1887 |
pobj |
vector,with |
R1153 |
T1890 |
T1889 |
amod |
linearized,vector |
R1154 |
T1891 |
T1889 |
compound |
targeting,vector |
R1155 |
T1892 |
T1889 |
punct |
", ",vector |
R1156 |
T1893 |
T1889 |
acl |
selected,vector |
R1157 |
T1894 |
T1893 |
prep |
in,selected |
R1158 |
T1895 |
T1896 |
det |
the,presence |
R1159 |
T1896 |
T1894 |
pobj |
presence,in |
R1160 |
T1897 |
T1896 |
prep |
of,presence |
R1161 |
T1898 |
T1897 |
pobj |
G418,of |
R1162 |
T1899 |
T1898 |
cc |
and,G418 |
R1163 |
T1900 |
T1898 |
conj |
FIAU,G418 |
R1164 |
T1901 |
T1885 |
punct |
", ",electroporated |
R1165 |
T1902 |
T1885 |
cc |
and,electroporated |
R1166 |
T1903 |
T1885 |
conj |
screened,electroporated |
R1167 |
T1904 |
T1903 |
prep |
for,screened |
R1168 |
T1905 |
T1906 |
amod |
correct,events |
R1169 |
T1906 |
T1904 |
pobj |
events,for |
R1170 |
T1907 |
T1906 |
compound |
integration,events |
R1171 |
T1908 |
T1903 |
prep |
by,screened |
R1172 |
T1909 |
T1910 |
nmod |
PCR,analysis |
R1173 |
T1910 |
T1908 |
pobj |
analysis,by |
R1174 |
T1911 |
T1909 |
cc |
and,PCR |
R1175 |
T1912 |
T1913 |
compound |
Southern,blot |
R1176 |
T1913 |
T1909 |
conj |
blot,PCR |
R1177 |
T1914 |
T1885 |
punct |
.,electroporated |
R1178 |
T1916 |
T1917 |
amod |
Transient,expression |
R1179 |
T1917 |
T1918 |
nsubj |
expression,led |
R1180 |
T1919 |
T1917 |
prep |
of,expression |
R1181 |
T1920 |
T1921 |
compound |
Cre,recombinase |
R1182 |
T1921 |
T1919 |
pobj |
recombinase,of |
R1183 |
T1922 |
T1918 |
prep |
to,led |
R1184 |
T1923 |
T1922 |
pobj |
removal,to |
R1185 |
T1924 |
T1923 |
prep |
of,removal |
R1186 |
T1925 |
T1926 |
det |
the,cassette |
R1187 |
T1926 |
T1924 |
pobj |
cassette,of |
R1188 |
T1927 |
T1928 |
compound |
PGK,neo |
R1189 |
T1928 |
T1926 |
compound |
neo,cassette |
R1190 |
T1929 |
T1928 |
punct |
-,neo |
R1191 |
T1930 |
T1918 |
punct |
", ",led |
R1192 |
T1931 |
T1918 |
cc |
and,led |
R1193 |
T1932 |
T1933 |
nsubjpass |
mice,generated |
R1194 |
T1933 |
T1918 |
conj |
generated,led |
R1195 |
T1934 |
T1932 |
acl |
carrying,mice |
R1196 |
T1935 |
T1936 |
det |
the,allele |
R1197 |
T1936 |
T1934 |
dobj |
allele,carrying |
R1198 |
T1937 |
T1936 |
amod |
modified,allele |
R1199 |
T1938 |
T1936 |
compound |
Mtf1loxP,allele |
R1200 |
T1939 |
T1933 |
auxpass |
were,generated |
R1201 |
T1940 |
T1933 |
prep |
by,generated |
R1202 |
T1941 |
T1940 |
pobj |
injection,by |
R1203 |
T1942 |
T1941 |
prep |
of,injection |
R1204 |
T1943 |
T1944 |
amod |
positive,clones |
R1205 |
T1944 |
T1942 |
pobj |
clones,of |
R1206 |
T1945 |
T1941 |
prep |
into,injection |
R1207 |
T1946 |
T1947 |
nmod |
C57Bl,blastocysts |
R1208 |
T1947 |
T1945 |
pobj |
blastocysts,into |
R1209 |
T1948 |
T1946 |
punct |
/,C57Bl |
R1210 |
T1949 |
T1946 |
nummod |
6,C57Bl |
R1211 |
T1950 |
T1947 |
cc |
and,blastocysts |
R1212 |
T1951 |
T1952 |
amod |
subsequent,crosses |
R1213 |
T1952 |
T1947 |
conj |
crosses,blastocysts |
R1214 |
T1953 |
T1933 |
punct |
.,generated |
R1215 |
T1955 |
T1956 |
amod |
Homozygous,animals |
R1216 |
T1956 |
T1959 |
nsubjpass |
animals,crossed |
R1217 |
T1957 |
T1958 |
amod |
conditional,knockout |
R1218 |
T1958 |
T1956 |
compound |
knockout,animals |
R1219 |
T1960 |
T1956 |
punct |
(,animals |
R1220 |
T1961 |
T1962 |
compound |
Mtf1loxP,loxP |
R1221 |
T1962 |
T1956 |
appos |
loxP,animals |
R1222 |
T1963 |
T1962 |
punct |
/,loxP |
R1223 |
T1964 |
T1959 |
punct |
),crossed |
R1224 |
T1965 |
T1959 |
auxpass |
were,crossed |
R1225 |
T1966 |
T1959 |
prep |
with,crossed |
R1226 |
T1967 |
T1968 |
det |
the,line |
R1227 |
T1968 |
T1966 |
pobj |
line,with |
R1228 |
T2074 |
T2075 |
compound |
Cre,recombinase |
R1229 |
T2075 |
T2076 |
npadvmod |
recombinase,transgenic |
R1230 |
T2076 |
T1968 |
amod |
transgenic,line |
R1231 |
T2077 |
T2078 |
compound |
Mx,cre |
R1232 |
T2078 |
T1968 |
appos |
cre,line |
R1233 |
T2079 |
T2078 |
punct |
-,cre |
R1234 |
T2080 |
T2081 |
punct |
(,gift |
R1235 |
T2081 |
T1968 |
parataxis |
gift,line |
R1236 |
T2082 |
T2081 |
det |
a,gift |
R1237 |
T2083 |
T2081 |
prep |
from,gift |
R1238 |
T2084 |
T2085 |
compound |
Prof.,Aguet |
R1239 |
T2085 |
T2083 |
pobj |
Aguet,from |
R1240 |
T2086 |
T2085 |
compound |
Michel,Aguet |
R1241 |
T2087 |
T2081 |
punct |
),gift |
R1242 |
T2088 |
T2089 |
aux |
to,obtain |
R1243 |
T2089 |
T1959 |
advcl |
obtain,crossed |
R1244 |
T2090 |
T2091 |
det |
an,line |
R1245 |
T2091 |
T2089 |
dobj |
line,obtain |
R1246 |
T2092 |
T2091 |
amod |
inducible,line |
R1247 |
T2093 |
T2091 |
punct |
", ",line |
R1248 |
T2094 |
T2095 |
npadvmod |
liver,specific |
R1249 |
T2095 |
T2091 |
amod |
specific,line |
R1250 |
T2096 |
T2095 |
punct |
-,specific |
R1251 |
T2097 |
T2098 |
compound |
Mtf1,knockout |
R1252 |
T2098 |
T2091 |
compound |
knockout,line |
R1253 |
T2099 |
T1959 |
punct |
.,crossed |
R1254 |
T2101 |
T2102 |
det |
The,mice |
R1255 |
T2102 |
T2103 |
nsubjpass |
mice,genotyped |
R1256 |
T2104 |
T2103 |
auxpass |
were,genotyped |
R1257 |
T2105 |
T2103 |
prep |
by,genotyped |
R1258 |
T2106 |
T2105 |
pobj |
PCR,by |
R1259 |
T2107 |
T2103 |
advcl |
using,genotyped |
R1260 |
T2108 |
T2109 |
det |
the,primers |
R1261 |
T2109 |
T2107 |
dobj |
primers,using |
R1262 |
T2110 |
T2109 |
amod |
following,primers |
R1263 |
T2111 |
T2112 |
punct |
(,Microsynth |
R1264 |
T2112 |
T2109 |
parataxis |
Microsynth,primers |
R1265 |
T2113 |
T2112 |
punct |
),Microsynth |
R1266 |
T2114 |
T2103 |
punct |
: ,genotyped |
R1267 |
T2115 |
T2116 |
compound |
Cre,recombinase |
R1268 |
T2116 |
T2117 |
dep |
recombinase,CCAGGTTACGGATATAGTTCATGAC |
R1269 |
T2117 |
T2130 |
dep |
CCAGGTTACGGATATAGTTCATGAC,CAGTCACAAGCAAATTACCAAACACTGCC |
R1270 |
T2118 |
T2117 |
punct |
: ,CCAGGTTACGGATATAGTTCATGAC |
R1271 |
T2119 |
T2120 |
nummod |
5,CTATCCAGCAACATTTGGGCCAGC |
R1272 |
T2120 |
T2117 |
dep |
CTATCCAGCAACATTTGGGCCAGC,CCAGGTTACGGATATAGTTCATGAC |
R1273 |
T2121 |
T2119 |
punct |
′,5 |
R1274 |
T2122 |
T2120 |
punct |
-,CTATCCAGCAACATTTGGGCCAGC |
R1275 |
T2123 |
T2120 |
punct |
-,CTATCCAGCAACATTTGGGCCAGC |
R1276 |
T2124 |
T2120 |
nummod |
3,CTATCCAGCAACATTTGGGCCAGC |
R1277 |
T2125 |
T2120 |
punct |
′,CTATCCAGCAACATTTGGGCCAGC |
R1278 |
T2126 |
T2117 |
punct |
;,CCAGGTTACGGATATAGTTCATGAC |
R1279 |
T2127 |
T2117 |
nummod |
5,CCAGGTTACGGATATAGTTCATGAC |
R1280 |
T2128 |
T2127 |
punct |
′,5 |
R1281 |
T2129 |
T2117 |
punct |
-,CCAGGTTACGGATATAGTTCATGAC |
R1282 |
T2130 |
T2103 |
dep |
CAGTCACAAGCAAATTACCAAACACTGCC,genotyped |
R1283 |
T2131 |
T2117 |
punct |
-,CCAGGTTACGGATATAGTTCATGAC |
R1284 |
T2132 |
T2117 |
nummod |
3,CCAGGTTACGGATATAGTTCATGAC |
R1285 |
T2133 |
T2117 |
punct |
′,CCAGGTTACGGATATAGTTCATGAC |
R1286 |
T2134 |
T2130 |
punct |
", ",CAGTCACAAGCAAATTACCAAACACTGCC |
R1287 |
T2135 |
T2130 |
dep |
Mtf1loxP,CAGTCACAAGCAAATTACCAAACACTGCC |
R1288 |
T2136 |
T2135 |
cc |
or,Mtf1loxP |
R1289 |
T2137 |
T2138 |
amod |
wild,type |
R1290 |
T2138 |
T2140 |
compound |
type,allele |
R1291 |
T2139 |
T2138 |
punct |
-,type |
R1292 |
T2140 |
T2135 |
conj |
allele,Mtf1loxP |
R1293 |
T2141 |
T2130 |
punct |
: ,CAGTCACAAGCAAATTACCAAACACTGCC |
R1294 |
T2142 |
T2143 |
nummod |
5,CACACCCAGTTTGTGTATGTCTTC |
R1295 |
T2143 |
T2130 |
dep |
CACACCCAGTTTGTGTATGTCTTC,CAGTCACAAGCAAATTACCAAACACTGCC |
R1296 |
T2144 |
T2142 |
punct |
′,5 |
R1297 |
T2145 |
T2143 |
punct |
-,CACACCCAGTTTGTGTATGTCTTC |
R1298 |
T2146 |
T2143 |
punct |
-,CACACCCAGTTTGTGTATGTCTTC |
R1299 |
T2147 |
T2143 |
nummod |
3,CACACCCAGTTTGTGTATGTCTTC |
R1300 |
T2148 |
T2143 |
punct |
′,CACACCCAGTTTGTGTATGTCTTC |
R1301 |
T2149 |
T2130 |
punct |
;,CAGTCACAAGCAAATTACCAAACACTGCC |
R1302 |
T2150 |
T2130 |
nummod |
5,CAGTCACAAGCAAATTACCAAACACTGCC |
R1303 |
T2151 |
T2150 |
punct |
′,5 |
R1304 |
T2152 |
T2130 |
punct |
-,CAGTCACAAGCAAATTACCAAACACTGCC |
R1305 |
T2153 |
T2130 |
punct |
-,CAGTCACAAGCAAATTACCAAACACTGCC |
R1306 |
T2154 |
T2130 |
nummod |
3,CAGTCACAAGCAAATTACCAAACACTGCC |
R1307 |
T2155 |
T2130 |
punct |
′,CAGTCACAAGCAAATTACCAAACACTGCC |
R1308 |
T2156 |
T2103 |
punct |
.,genotyped |
R1309 |
T2173 |
T2174 |
compound |
Animal,treatment |
R1310 |
T2176 |
T2177 |
prep |
At,received |
R1311 |
T2178 |
T2179 |
nummod |
8,weeks |
R1312 |
T2179 |
T2176 |
pobj |
weeks,At |
R1313 |
T2180 |
T2179 |
prep |
of,weeks |
R1314 |
T2181 |
T2180 |
pobj |
age,of |
R1315 |
T2182 |
T2177 |
punct |
", ",received |
R1316 |
T2183 |
T2184 |
amod |
male,mice |
R1317 |
T2184 |
T2177 |
nsubj |
mice,received |
R1318 |
T2185 |
T2186 |
compound |
Mtf1loxP,loxP |
R1319 |
T2186 |
T2184 |
compound |
loxP,mice |
R1320 |
T2187 |
T2186 |
punct |
/,loxP |
R1321 |
T2188 |
T2184 |
acl |
harboring,mice |
R1322 |
T2189 |
T2190 |
det |
the,transgene |
R1323 |
T2190 |
T2188 |
dobj |
transgene,harboring |
R1324 |
T2191 |
T2192 |
compound |
Mx,cre |
R1325 |
T2192 |
T2190 |
compound |
cre,transgene |
R1326 |
T2193 |
T2192 |
punct |
-,cre |
R1327 |
T2194 |
T2190 |
punct |
(,transgene |
R1328 |
T2195 |
T2196 |
compound |
Mtf1loxP,cre |
R1330 |
T2197 |
T2196 |
punct |
/,cre |
R1331 |
T2198 |
T2196 |
compound |
loxP,cre |
R1332 |
T2199 |
T2196 |
compound |
Mx,cre |
R1333 |
T2200 |
T2196 |
punct |
-,cre |
R1334 |
T2201 |
T2196 |
punct |
", ",cre |
R1335 |
T2202 |
T2203 |
advmod |
abbr.,cre |
R1336 |
T2203 |
T2196 |
appos |
cre,cre |
R1337 |
T2204 |
T2203 |
punct |
: ,cre |
R1338 |
T2205 |
T2203 |
compound |
Mtf1Mx,cre |
R1339 |
T2206 |
T2203 |
punct |
-,cre |
R1340 |
T2207 |
T2190 |
punct |
),transgene |
R1341 |
T2208 |
T2190 |
cc |
and,transgene |
R1342 |
T2209 |
T2210 |
compound |
control,littermates |
R1343 |
T2210 |
T2190 |
conj |
littermates,transgene |
R1344 |
T2211 |
T2210 |
prep |
without,littermates |
R1345 |
T2212 |
T2211 |
pobj |
transgene,without |
R1346 |
T2213 |
T2210 |
punct |
(,littermates |
R1347 |
T2214 |
T2215 |
compound |
Mtf1loxP,loxP |
R1348 |
T2215 |
T2210 |
appos |
loxP,littermates |
R1349 |
T2216 |
T2215 |
punct |
/,loxP |
R1350 |
T2217 |
T2215 |
punct |
", ",loxP |
R1351 |
T2218 |
T2219 |
advmod |
abbr.,Mtf1loxP |
R1352 |
T2219 |
T2215 |
appos |
Mtf1loxP,loxP |
R1353 |
T2220 |
T2219 |
punct |
: ,Mtf1loxP |
R1354 |
T2221 |
T2177 |
punct |
),received |
R1355 |
T2222 |
T2223 |
nummod |
four,injections |
R1356 |
T2223 |
T2177 |
dobj |
injections,received |
R1357 |
T2224 |
T2223 |
amod |
intraperitoneal,injections |
R1358 |
T2225 |
T2223 |
advmod |
each,injections |
R1359 |
T2226 |
T2223 |
prep |
of,injections |
R1360 |
T2227 |
T2228 |
nummod |
300,µg |
R1361 |
T2228 |
T2229 |
nmod |
µg,acid |
R1362 |
T2229 |
T2226 |
pobj |
acid,of |
R1363 |
T2230 |
T2229 |
amod |
synthetic,acid |
R1364 |
T2231 |
T2232 |
amod |
double,stranded |
R1365 |
T2232 |
T2229 |
amod |
stranded,acid |
R1366 |
T2233 |
T2323 |
nummod |
20,µmol |
R1367 |
T2234 |
T2232 |
punct |
-,stranded |
R1368 |
T2235 |
T2229 |
nmod |
RNA,acid |
R1369 |
T2236 |
T2237 |
amod |
polyinosinic,polycytidylic |
R1370 |
T2237 |
T2229 |
amod |
polycytidylic,acid |
R1371 |
T2238 |
T2237 |
punct |
–,polycytidylic |
R1372 |
T2239 |
T2240 |
punct |
[,Sigma |
R1373 |
T2240 |
T2229 |
parataxis |
Sigma,acid |
R1374 |
T2241 |
T2242 |
compound |
pI,pC |
R1375 |
T2242 |
T2240 |
dep |
pC,Sigma |
R1376 |
T2243 |
T2242 |
punct |
–,pC |
R1377 |
T2244 |
T2240 |
punct |
;,Sigma |
R1378 |
T2245 |
T2240 |
punct |
;,Sigma |
R1379 |
T2246 |
T2240 |
prep |
in,Sigma |
R1380 |
T2247 |
T2248 |
det |
a,volume |
R1381 |
T2248 |
T2246 |
pobj |
volume,in |
R1382 |
T2249 |
T2248 |
prep |
of,volume |
R1383 |
T2250 |
T2251 |
nummod |
60,µl |
R1384 |
T2251 |
T2249 |
pobj |
µl,of |
R1385 |
T2252 |
T2253 |
npadvmod |
phosphate,buffered |
R1386 |
T2253 |
T2255 |
amod |
buffered,saline |
R1387 |
T2254 |
T2253 |
punct |
-,buffered |
R1388 |
T2255 |
T2251 |
appos |
saline,µl |
R1389 |
T2256 |
T2255 |
punct |
(,saline |
R1390 |
T2257 |
T2255 |
appos |
PBS,saline |
R1391 |
T2258 |
T2240 |
punct |
),Sigma |
R1392 |
T2259 |
T2240 |
punct |
],Sigma |
R1393 |
T2260 |
T2177 |
prep |
at,received |
R1394 |
T2261 |
T2262 |
nummod |
3,day |
R1395 |
T2262 |
T2263 |
compound |
day,intervals |
R1396 |
T2263 |
T2260 |
pobj |
intervals,at |
R1397 |
T2264 |
T2177 |
punct |
.,received |
R1398 |
T2266 |
T2267 |
advmod |
Only,in |
R1399 |
T2267 |
T2268 |
prep |
in,received |
R1400 |
T2269 |
T2270 |
det |
the,case |
R1401 |
T2270 |
T2267 |
pobj |
case,in |
R1402 |
T2271 |
T2270 |
prep |
of,case |
R1403 |
T2272 |
T2273 |
npadvmod |
DNA,binding |
R1404 |
T2273 |
T2275 |
amod |
binding,studies |
R1405 |
T2274 |
T2273 |
punct |
-,binding |
R1406 |
T2275 |
T2271 |
pobj |
studies,of |
R1407 |
T2276 |
T2275 |
prep |
with,studies |
R1408 |
T2277 |
T2278 |
compound |
MRE,sequences |
R1409 |
T2278 |
T2276 |
pobj |
sequences,with |
R1410 |
T2279 |
T2278 |
prep |
from,sequences |
R1411 |
T2280 |
T2281 |
nmod |
MTF,candidates |
R1412 |
T2281 |
T2279 |
pobj |
candidates,from |
R1413 |
T2282 |
T2280 |
punct |
-,MTF |
R1414 |
T2283 |
T2280 |
nummod |
1,MTF |
R1415 |
T2284 |
T2285 |
compound |
target,gene |
R1416 |
T2285 |
T2281 |
compound |
gene,candidates |
R1417 |
T2286 |
T2268 |
punct |
", ",received |
R1418 |
T2287 |
T2288 |
det |
the,mice |
R1419 |
T2288 |
T2268 |
nsubj |
mice,received |
R1420 |
T2289 |
T2288 |
compound |
control,mice |
R1421 |
T2290 |
T2291 |
det |
no,injections |
R1422 |
T2291 |
T2268 |
dobj |
injections,received |
R1423 |
T2292 |
T2293 |
compound |
pI,pC |
R1424 |
T2293 |
T2291 |
compound |
pC,injections |
R1425 |
T2294 |
T2293 |
punct |
–,pC |
R1426 |
T2295 |
T2268 |
punct |
.,received |
R1427 |
T2297 |
T2298 |
prep |
For,received |
R1428 |
T2299 |
T2297 |
pobj |
experiments,For |
R1429 |
T2300 |
T2299 |
prep |
with,experiments |
R1430 |
T2301 |
T2302 |
compound |
metal,treatment |
R1431 |
T2302 |
T2300 |
pobj |
treatment,with |
R1432 |
T2303 |
T2298 |
punct |
", ",received |
R1433 |
T2304 |
T2298 |
nsubj |
mice,received |
R1434 |
T2305 |
T2306 |
nummod |
2,days |
R1435 |
T2306 |
T2307 |
npadvmod |
days,after |
R1436 |
T2307 |
T2298 |
prep |
after,received |
R1437 |
T2308 |
T2309 |
det |
the,treatment |
R1438 |
T2309 |
T2307 |
pobj |
treatment,after |
R1439 |
T2310 |
T2309 |
amod |
last,treatment |
R1440 |
T2311 |
T2312 |
compound |
pI,pC |
R1441 |
T2312 |
T2309 |
compound |
pC,treatment |
R1442 |
T2313 |
T2312 |
punct |
–,pC |
R1443 |
T2314 |
T2315 |
det |
a,injection |
R1444 |
T2315 |
T2298 |
dobj |
injection,received |
R1445 |
T2316 |
T2317 |
amod |
subcutaneous,s.c. |
R1446 |
T2317 |
T2315 |
amod |
s.c.,injection |
R1447 |
T2318 |
T2317 |
punct |
(,s.c. |
R1448 |
T2319 |
T2315 |
punct |
),injection |
R1449 |
T2320 |
T2315 |
prep |
of,injection |
R1450 |
T2321 |
T2322 |
preconj |
either,CdSO4 |
R1451 |
T2322 |
T2320 |
pobj |
CdSO4,of |
R1452 |
T2323 |
T2322 |
nmod |
µmol,CdSO4 |
R1453 |
T2324 |
T2325 |
punct |
/,weight |
R1454 |
T2325 |
T2323 |
prep |
weight,µmol |
R1455 |
T2326 |
T2325 |
compound |
kg,weight |
R1456 |
T2327 |
T2325 |
compound |
body,weight |
R1457 |
T2328 |
T2329 |
punct |
(,treatment |
R1458 |
T2329 |
T2322 |
parataxis |
treatment,CdSO4 |
R1459 |
T2330 |
T2331 |
nummod |
2,mM |
R1460 |
T2331 |
T2332 |
compound |
mM,CdSO4 |
R1461 |
T2332 |
T2329 |
dep |
CdSO4,treatment |
R1462 |
T2333 |
T2332 |
prep |
in,CdSO4 |
R1463 |
T2334 |
T2333 |
pobj |
H2O,in |
R1464 |
T2335 |
T2329 |
punct |
;,treatment |
R1465 |
T2336 |
T2329 |
compound |
cadmium,treatment |
R1466 |
T2337 |
T2329 |
punct |
),treatment |
R1467 |
T2338 |
T2322 |
cc |
or,CdSO4 |
R1468 |
T2339 |
T2340 |
nummod |
10,ml |
R1469 |
T2340 |
T2341 |
nmod |
ml,H2O |
R1471 |
T2342 |
T2343 |
punct |
/,weight |
R1472 |
T2343 |
T2340 |
prep |
weight,ml |
R1473 |
T2344 |
T2343 |
compound |
kg,weight |
R1474 |
T2345 |
T2343 |
compound |
body,weight |
R1475 |
T2346 |
T2347 |
punct |
(,treatment |
R1476 |
T2347 |
T2341 |
parataxis |
treatment,H2O |
R1477 |
T2348 |
T2347 |
amod |
mock,treatment |
R1478 |
T2349 |
T2347 |
punct |
),treatment |
R1479 |
T2350 |
T2351 |
nummod |
6,h |
R1480 |
T2351 |
T2352 |
npadvmod |
h,before |
R1481 |
T2352 |
T2298 |
prep |
before,received |
R1482 |
T2353 |
T2352 |
pcomp |
sacrificing,before |
R1483 |
T2354 |
T2353 |
dobj |
them,sacrificing |
R1484 |
T2355 |
T2298 |
punct |
.,received |
R1487 |
T2531 |
T2532 |
compound |
Microarray,analysis |
R1488 |
T2533 |
T2532 |
cc |
and,analysis |
R1489 |
T2534 |
T2535 |
compound |
data,processing |
R1490 |
T2535 |
T2532 |
conj |
processing,analysis |
R1491 |
T2537 |
T2538 |
amod |
Total,RNA |
R1492 |
T2538 |
T2539 |
nsubjpass |
RNA,isolated |
R1493 |
T2540 |
T2539 |
auxpass |
was,isolated |
R1494 |
T2541 |
T2539 |
prep |
from,isolated |
R1495 |
T2542 |
T2543 |
compound |
liver,tissue |
R1496 |
T2543 |
T2541 |
pobj |
tissue,from |
R1497 |
T2544 |
T2543 |
prep |
of,tissue |
R1498 |
T2545 |
T2546 |
compound |
pI,pC |
R1499 |
T2546 |
T2548 |
npadvmod |
pC,induced |
R1500 |
T2547 |
T2546 |
punct |
–,pC |
R1501 |
T2548 |
T2550 |
amod |
induced,mice |
R1502 |
T2549 |
T2548 |
punct |
-,induced |
R1503 |
T2550 |
T2544 |
pobj |
mice,of |
R1504 |
T2551 |
T2550 |
punct |
", ",mice |
R1505 |
T2552 |
T2553 |
amod |
mock,treated |
R1506 |
T2553 |
T2550 |
amod |
treated,mice |
R1507 |
T2554 |
T2552 |
punct |
-,mock |
R1508 |
T2555 |
T2552 |
cc |
or,mock |
R1509 |
T2556 |
T2552 |
conj |
cadmium,mock |
R1510 |
T2557 |
T2553 |
punct |
-,treated |
R1511 |
T2558 |
T2559 |
nmod |
Mtf1Mx,cre |
R1512 |
T2559 |
T2550 |
nmod |
cre,mice |
R1513 |
T2560 |
T2559 |
punct |
-,cre |
R1514 |
T2561 |
T2559 |
cc |
and,cre |
R1515 |
T2562 |
T2559 |
conj |
Mtf1loxP,cre |
R1516 |
T2563 |
T2564 |
punct |
(,male |
R1517 |
T2564 |
T2544 |
parataxis |
male,of |
R1518 |
T2565 |
T2566 |
nsubj |
n,3 |
R1519 |
T2566 |
T2564 |
ccomp |
3,male |
R1520 |
T2567 |
T2566 |
punct |
=,3 |
R1521 |
T2568 |
T2566 |
prep |
per,3 |
R1522 |
T2569 |
T2568 |
pobj |
genotype,per |
R1523 |
T2570 |
T2569 |
cc |
and,genotype |
R1524 |
T2571 |
T2572 |
amod |
respective,treatment |
R1525 |
T2572 |
T2569 |
conj |
treatment,genotype |
R1526 |
T2573 |
T2564 |
punct |
;,male |
R1527 |
T2574 |
T2564 |
det |
all,male |
R1528 |
T2575 |
T2564 |
punct |
),male |
R1529 |
T2576 |
T2577 |
advmod |
essentially,described |
R1530 |
T2577 |
T2539 |
advcl |
described,isolated |
R1531 |
T2578 |
T2577 |
mark |
as,described |
R1532 |
T2579 |
T2577 |
agent |
by,described |
R1533 |
T2580 |
T2579 |
pobj |
Chomczynski,by |
R1534 |
T2581 |
T2580 |
cc |
and,Chomczynski |
R1535 |
T2582 |
T2580 |
conj |
Sacchi,Chomczynski |
R1536 |
T2583 |
T2584 |
punct |
(,27 |
R1537 |
T2584 |
T2577 |
parataxis |
27,described |
R1538 |
T2585 |
T2584 |
punct |
),27 |
R1539 |
T2586 |
T2539 |
punct |
.,isolated |
R1540 |
T2588 |
T2589 |
compound |
Gene,expression |
R1541 |
T2589 |
T2590 |
compound |
expression,analysis |
R1542 |
T2590 |
T2591 |
nsubjpass |
analysis,performed |
R1543 |
T2592 |
T2591 |
auxpass |
was,performed |
R1544 |
T2593 |
T2591 |
prep |
in,performed |
R1545 |
T2594 |
T2595 |
det |
the,Center |
R1546 |
T2595 |
T2593 |
pobj |
Center,in |
R1547 |
T2596 |
T2597 |
compound |
Functional,Genomics |
R1548 |
T2597 |
T2595 |
compound |
Genomics,Center |
R1549 |
T2598 |
T2595 |
npadvmod |
Zurich,Center |
R1550 |
T2599 |
T2591 |
advcl |
using,performed |
R1551 |
T2600 |
T2601 |
nmod |
GeneChip®,Arrays |
R1552 |
T2601 |
T2599 |
dobj |
Arrays,using |
R1553 |
T2602 |
T2603 |
nmod |
Mouse,Genome |
R1554 |
T2603 |
T2601 |
nmod |
Genome,Arrays |
R1555 |
T2604 |
T2603 |
nummod |
430,Genome |
R1556 |
T2605 |
T2601 |
nummod |
2.0,Arrays |
R1557 |
T2606 |
T2607 |
punct |
(,Affymetrix |
R1558 |
T2607 |
T2601 |
parataxis |
Affymetrix,Arrays |
R1559 |
T2608 |
T2607 |
punct |
),Affymetrix |
R1560 |
T2609 |
T2599 |
prep |
according,using |
R1561 |
T2610 |
T2609 |
prep |
to,according |
R1562 |
T2611 |
T2612 |
det |
the,manufacturer |
R1563 |
T2612 |
T2613 |
poss |
manufacturer,instructions |
R1564 |
T2613 |
T2610 |
pobj |
instructions,to |
R1565 |
T2614 |
T2612 |
case |
's,manufacturer |
R1566 |
T2615 |
T2613 |
cc |
and,instructions |
R1567 |
T2616 |
T2617 |
det |
the,reagents |
R1568 |
T2617 |
T2613 |
conj |
reagents,instructions |
R1569 |
T2618 |
T2617 |
amod |
following,reagents |
R1570 |
T2619 |
T2617 |
cc |
and,reagents |
R1571 |
T2620 |
T2617 |
conj |
conditions,reagents |
R1572 |
T2621 |
T2591 |
punct |
.,performed |
R1573 |
T2623 |
T2624 |
nsubjpass |
cDNA,synthesized |
R1574 |
T2625 |
T2624 |
auxpass |
was,synthesized |
R1575 |
T2626 |
T2624 |
prep |
with,synthesized |
R1576 |
T2627 |
T2628 |
nmod |
SuperScript,Kit |
R1578 |
T2629 |
T2627 |
punct |
™,SuperScript |
R1579 |
T2630 |
T2631 |
amod |
Double,Stranded |
R1580 |
T2631 |
T2628 |
amod |
Stranded,Kit |
R1581 |
T2632 |
T2631 |
punct |
-,Stranded |
R1582 |
T2633 |
T2628 |
compound |
cDNA,Kit |
R1583 |
T2634 |
T2628 |
compound |
Synthesis,Kit |
R1584 |
T2635 |
T2636 |
punct |
(,Invitrogen |
R1585 |
T2636 |
T2628 |
parataxis |
Invitrogen,Kit |
R1586 |
T2637 |
T2636 |
punct |
),Invitrogen |
R1587 |
T2638 |
T2624 |
punct |
", ",synthesized |
R1588 |
T2639 |
T2624 |
advcl |
using,synthesized |
R1589 |
T2640 |
T2641 |
nummod |
15,µg |
R1590 |
T2641 |
T2642 |
nmod |
µg,RNA |
R1591 |
T2642 |
T2639 |
dobj |
RNA,using |
R1592 |
T2643 |
T2642 |
amod |
total,RNA |
R1593 |
T2644 |
T2624 |
punct |
.,synthesized |
R1594 |
T2646 |
T2647 |
advmod |
In,vitro |
R1595 |
T2647 |
T2648 |
amod |
vitro,transcription |
R1596 |
T2648 |
T2649 |
nsubjpass |
transcription,performed |
R1597 |
T2650 |
T2649 |
auxpass |
was,performed |
R1598 |
T2651 |
T2649 |
prep |
with,performed |
R1599 |
T2652 |
T2653 |
nmod |
BioArray,Kit |
R1600 |
T2653 |
T2651 |
pobj |
Kit,with |
R1601 |
T2654 |
T2652 |
punct |
™,BioArray |
R1602 |
T2655 |
T2656 |
nmod |
High,Yield |
R1603 |
T2656 |
T2653 |
nmod |
Yield,Kit |
R1604 |
T2657 |
T2656 |
punct |
™,Yield |
R1605 |
T2658 |
T2653 |
compound |
RNA,Kit |
R1606 |
T2659 |
T2653 |
compound |
Transcript,Kit |
R1607 |
T2660 |
T2653 |
compound |
Labeling,Kit |
R1608 |
T2661 |
T2662 |
punct |
(,Enzo |
R1609 |
T2662 |
T2653 |
parataxis |
Enzo,Kit |
R1610 |
T2663 |
T2662 |
punct |
),Enzo |
R1611 |
T2664 |
T2653 |
cc |
and,Kit |
R1612 |
T2665 |
T2666 |
quantmod |
3.5,6 |
R1613 |
T2666 |
T2668 |
nummod |
6,µg |
R1614 |
T2667 |
T2666 |
quantmod |
to,6 |
R1615 |
T2668 |
T2653 |
conj |
µg,Kit |
R1616 |
T2669 |
T2668 |
prep |
of,µg |
R1617 |
T2670 |
T2671 |
det |
each,cDNA |
R1618 |
T2671 |
T2669 |
pobj |
cDNA,of |
R1619 |
T2672 |
T2649 |
punct |
.,performed |
R1620 |
T2674 |
T2675 |
compound |
Clean,up |
R1621 |
T2675 |
T2677 |
nsubjpass |
up,done |
R1622 |
T2676 |
T2675 |
punct |
-,up |
R1623 |
T2678 |
T2675 |
prep |
of,up |
R1624 |
T2679 |
T2680 |
preconj |
both,cDNA |
R1625 |
T2680 |
T2681 |
nmod |
cDNA,samples |
R1626 |
T2681 |
T2678 |
pobj |
samples,of |
R1627 |
T2682 |
T2680 |
cc |
and,cDNA |
R1628 |
T2683 |
T2680 |
conj |
cRNA,cDNA |
R1629 |
T2684 |
T2677 |
auxpass |
was,done |
R1630 |
T2685 |
T2677 |
advcl |
using,done |
R1631 |
T2686 |
T2687 |
compound |
GeneChip®,Module |
R1632 |
T2687 |
T2685 |
dobj |
Module,using |
R1633 |
T2688 |
T2687 |
compound |
Sample,Module |
R1634 |
T2689 |
T2690 |
compound |
Clean,up |
R1635 |
T2690 |
T2687 |
compound |
up,Module |
R1636 |
T2691 |
T2690 |
punct |
-,up |
R1637 |
T2692 |
T2693 |
punct |
(,Affymetrix |
R1638 |
T2693 |
T2687 |
parataxis |
Affymetrix,Module |
R1639 |
T2694 |
T2693 |
punct |
),Affymetrix |
R1640 |
T2695 |
T2677 |
punct |
.,done |
R1641 |
T2697 |
T2698 |
prep |
For,used |
R1642 |
T2699 |
T2700 |
det |
the,washing |
R1643 |
T2700 |
T2697 |
pobj |
washing,For |
R1644 |
T2701 |
T2700 |
amod |
automated,washing |
R1645 |
T2702 |
T2700 |
cc |
and,washing |
R1646 |
T2703 |
T2700 |
conj |
staining,washing |
R1647 |
T2704 |
T2700 |
prep |
in,washing |
R1648 |
T2705 |
T2706 |
det |
the,station |
R1649 |
T2706 |
T2704 |
pobj |
station,in |
R1650 |
T2707 |
T2706 |
compound |
Affymetrix,station |
R1651 |
T2708 |
T2706 |
compound |
fluidics,station |
R1652 |
T2709 |
T2706 |
nummod |
450,station |
R1653 |
T2710 |
T2698 |
punct |
", ",used |
R1654 |
T2711 |
T2712 |
det |
the,Ws2v4_450 |
R1655 |
T2712 |
T2698 |
nsubjpass |
Ws2v4_450,used |
R1656 |
T2713 |
T2712 |
compound |
protocol,Ws2v4_450 |
R1657 |
T2714 |
T2712 |
compound |
EukGE,Ws2v4_450 |
R1658 |
T2715 |
T2712 |
punct |
-,Ws2v4_450 |
R1659 |
T2716 |
T2698 |
auxpass |
was,used |
R1660 |
T2717 |
T2698 |
punct |
.,used |
R1661 |
T2719 |
T2720 |
det |
The,arrays |
R1662 |
T2720 |
T2722 |
nsubjpass |
arrays,scanned |
R1663 |
T2721 |
T2720 |
compound |
probe,arrays |
R1664 |
T2723 |
T2722 |
auxpass |
were,scanned |
R1665 |
T2724 |
T2722 |
prep |
with,scanned |
R1666 |
T2725 |
T2726 |
det |
the,scanner |
R1667 |
T2726 |
T2724 |
pobj |
scanner,with |
R1668 |
T2727 |
T2728 |
nmod |
Affymetrix,GS |
R1669 |
T2728 |
T2726 |
nmod |
GS,scanner |
R1670 |
T2729 |
T2728 |
nummod |
3000,GS |
R1671 |
T2730 |
T2722 |
punct |
.,scanned |
R1672 |
T2732 |
T2733 |
amod |
Raw,data |
R1673 |
T2733 |
T2734 |
nsubj |
data,are |
R1674 |
T2735 |
T2734 |
acomp |
available,are |
R1675 |
T2736 |
T2734 |
prep |
at,are |
R1676 |
T2737 |
T2736 |
pobj |
ArrayExpress,at |
R1677 |
T2738 |
T2739 |
punct |
(,MEXP |
R1678 |
T2739 |
T2734 |
parataxis |
MEXP,are |
R1679 |
T2740 |
T2739 |
punct |
", ",MEXP |
R1680 |
T2741 |
T2742 |
compound |
accession,number |
R1681 |
T2742 |
T2739 |
compound |
number,MEXP |
R1682 |
T2743 |
T2739 |
compound |
E,MEXP |
R1683 |
T2744 |
T2739 |
punct |
-,MEXP |
R1684 |
T2745 |
T2739 |
punct |
-,MEXP |
R1685 |
T2746 |
T2739 |
nummod |
438,MEXP |
R1686 |
T2747 |
T2739 |
punct |
),MEXP |
R1687 |
T2748 |
T2734 |
punct |
.,are |
R1688 |
T2750 |
T2751 |
compound |
Data,analysis |
R1689 |
T2751 |
T2752 |
nsubjpass |
analysis,performed |
R1690 |
T2753 |
T2752 |
auxpass |
was,performed |
R1691 |
T2754 |
T2752 |
prep |
with,performed |
R1692 |
T2755 |
T2756 |
nmod |
GeneSpring,software |
R1693 |
T2756 |
T2754 |
pobj |
software,with |
R1694 |
T2757 |
T2756 |
nummod |
6.1,software |
R1695 |
T2758 |
T2759 |
punct |
(,Genetics |
R1696 |
T2759 |
T2756 |
parataxis |
Genetics,software |
R1697 |
T2760 |
T2759 |
compound |
Silicon,Genetics |
R1698 |
T2761 |
T2759 |
punct |
),Genetics |
R1699 |
T2762 |
T2752 |
punct |
", ",performed |
R1700 |
T2763 |
T2752 |
advcl |
applying,performed |
R1701 |
T2764 |
T2765 |
det |
a,level |
R1702 |
T2765 |
T2763 |
dobj |
level,applying |
R1703 |
T2766 |
T2765 |
compound |
significance,level |
R1704 |
T2767 |
T2768 |
nsubj |
P,0.05 |
R1705 |
T2768 |
T2765 |
appos |
0.05,level |
R1706 |
T2769 |
T2768 |
punct |
≤,0.05 |
R1707 |
T2770 |
T2752 |
punct |
.,performed |
R1708 |
T2772 |
T2773 |
advmod |
Furthermore,used |
R1709 |
T2774 |
T2773 |
punct |
", ",used |
R1710 |
T2775 |
T2776 |
amod |
multiple,correction |
R1711 |
T2776 |
T2773 |
nsubjpass |
correction,used |
R1712 |
T2777 |
T2776 |
compound |
testing,correction |
R1713 |
T2778 |
T2773 |
auxpass |
was,used |
R1714 |
T2779 |
T2773 |
prep |
in,used |
R1715 |
T2780 |
T2779 |
pobj |
addition,in |
R1716 |
T2781 |
T2782 |
aux |
to,obtain |
R1717 |
T2782 |
T2773 |
advcl |
obtain,used |
R1718 |
T2783 |
T2784 |
npadvmod |
cadmium,responsive |
R1719 |
T2784 |
T2786 |
amod |
responsive,genes |
R1720 |
T2785 |
T2784 |
punct |
-,responsive |
R1722 |
T2787 |
T2786 |
punct |
", ",genes |
R1723 |
T2788 |
T2789 |
npadvmod |
MTF,independent |
R1724 |
T2789 |
T2786 |
amod |
independent,genes |
R1725 |
T2790 |
T2788 |
punct |
-,MTF |
R1726 |
T2791 |
T2788 |
nummod |
1,MTF |
R1727 |
T2792 |
T2789 |
punct |
-,independent |
R1728 |
T2793 |
T2773 |
punct |
.,used |
R1729 |
T2795 |
T2796 |
nsubjpass |
Genes,considered |
R1730 |
T2797 |
T2796 |
auxpass |
were,considered |
R1731 |
T2798 |
T2799 |
aux |
to,expressed |
R1732 |
T2799 |
T2796 |
xcomp |
expressed,considered |
R1733 |
T2800 |
T2799 |
auxpass |
be,expressed |
R1734 |
T2801 |
T2799 |
advmod |
differentially,expressed |
R1735 |
T2802 |
T2803 |
mark |
if,was |
R1736 |
T2803 |
T2799 |
advcl |
was,expressed |
R1737 |
T2804 |
T2803 |
expl |
there,was |
R1738 |
T2805 |
T2806 |
advmod |
at,least |
R1739 |
T2806 |
T2807 |
advmod |
least,2-fold |
R1740 |
T2807 |
T2809 |
nummod |
2-fold,difference |
R1741 |
T2808 |
T2807 |
quantmod |
a,2-fold |
R1742 |
T2809 |
T2803 |
attr |
difference,was |
R1743 |
T2810 |
T2809 |
prep |
in,difference |
R1744 |
T2811 |
T2812 |
compound |
expression,levels |
R1745 |
T2812 |
T2810 |
pobj |
levels,in |
R1746 |
T2813 |
T2812 |
prep |
of,levels |
R1747 |
T2814 |
T2815 |
det |
the,groups |
R1748 |
T2815 |
T2813 |
pobj |
groups,of |
R1749 |
T2816 |
T2815 |
amod |
compared,groups |
R1750 |
T2817 |
T2815 |
amod |
experimental,groups |
R1751 |
T2818 |
T2819 |
punct |
(,genotype |
R1752 |
T2819 |
T2803 |
parataxis |
genotype,was |
R1753 |
T2820 |
T2819 |
cc |
and,genotype |
R1754 |
T2821 |
T2822 |
amod |
respective,treatment |
R1755 |
T2822 |
T2819 |
conj |
treatment,genotype |
R1756 |
T2823 |
T2819 |
punct |
),genotype |
R1757 |
T2824 |
T2796 |
punct |
.,considered |
R1758 |
T2826 |
T2827 |
det |
The,result |
R1759 |
T2827 |
T2828 |
nsubjpass |
result,considered |
R1760 |
T2829 |
T2828 |
auxpass |
was,considered |
R1761 |
T2830 |
T2828 |
oprd |
reliable,considered |
R1762 |
T2831 |
T2832 |
mark |
if,scored |
R1763 |
T2832 |
T2828 |
advcl |
scored,considered |
R1764 |
T2833 |
T2834 |
compound |
signal,values |
R1765 |
T2834 |
T2832 |
nsubjpass |
values,scored |
R1766 |
T2835 |
T2834 |
prep |
for,values |
R1767 |
T2836 |
T2837 |
det |
the,gene |
R1768 |
T2837 |
T2835 |
pobj |
gene,for |
R1769 |
T2838 |
T2837 |
amod |
respective,gene |
R1770 |
T2839 |
T2832 |
auxpass |
were,scored |
R1771 |
T2840 |
T2841 |
punct |
‘,present |
R1772 |
T2841 |
T2832 |
oprd |
present,scored |
R1773 |
T2842 |
T2832 |
punct |
’,scored |
R1774 |
T2843 |
T2844 |
advmod |
at,least |
R1775 |
T2844 |
T2845 |
advmod |
least,for |
R1776 |
T2845 |
T2828 |
prep |
for,considered |
R1777 |
T2846 |
T2847 |
det |
all,mice |
R1778 |
T2847 |
T2845 |
pobj |
mice,for |
R1779 |
T2848 |
T2847 |
prep |
in,mice |
R1780 |
T2849 |
T2850 |
nummod |
one,group |
R1781 |
T2850 |
T2848 |
pobj |
group,in |
R1782 |
T2851 |
T2850 |
amod |
experimental,group |
R1783 |
T2852 |
T2845 |
cc |
or,for |
R1784 |
T2853 |
T2845 |
conj |
for,for |
R1785 |
T2854 |
T2855 |
nummod |
two,mice |
R1786 |
T2855 |
T2853 |
pobj |
mice,for |
R1787 |
T2856 |
T2855 |
prep |
in,mice |
R1788 |
T2857 |
T2856 |
pobj |
each,in |
R1789 |
T2858 |
T2857 |
prep |
of,each |
R1790 |
T2859 |
T2860 |
nummod |
two,groups |
R1791 |
T2860 |
T2858 |
pobj |
groups,of |
R1792 |
T2861 |
T2828 |
punct |
.,considered |
R1793 |
T2863 |
T2864 |
aux |
To,screen |
R1794 |
T2864 |
T2865 |
advcl |
screen,obtained |
R1795 |
T2866 |
T2864 |
prep |
for,screen |
R1796 |
T2867 |
T2868 |
det |
the,presence |
R1797 |
T2868 |
T2866 |
pobj |
presence,for |
R1798 |
T2869 |
T2868 |
prep |
of,presence |
R1799 |
T2870 |
T2871 |
det |
the,sequence |
R1800 |
T2871 |
T2869 |
pobj |
sequence,of |
R1801 |
T2872 |
T2871 |
compound |
MRE,sequence |
R1802 |
T2873 |
T2871 |
compound |
core,sequence |
R1803 |
T2874 |
T2871 |
compound |
consensus,sequence |
R1804 |
T2875 |
T2871 |
appos |
TGCRCNC,sequence |
R1805 |
T2876 |
T2868 |
prep |
in,presence |
R1806 |
T2877 |
T2878 |
det |
the,region |
R1807 |
T2878 |
T2876 |
pobj |
region,in |
R1808 |
T2879 |
T2878 |
compound |
promoter,region |
R1809 |
T2880 |
T2865 |
punct |
", ",obtained |
R1810 |
T2881 |
T2882 |
det |
the,sequences |
R1811 |
T2882 |
T2865 |
nsubjpass |
sequences,obtained |
R1812 |
T2883 |
T2882 |
amod |
upstream,sequences |
R1813 |
T2884 |
T2882 |
prep |
of,sequences |
R1814 |
T2885 |
T2886 |
det |
the,genes |
R1815 |
T2886 |
T2884 |
pobj |
genes,of |
R1816 |
T2887 |
T2886 |
amod |
respective,genes |
R1817 |
T2888 |
T2865 |
auxpass |
were,obtained |
R1818 |
T2889 |
T2865 |
prep |
from,obtained |
R1819 |
T2890 |
T2891 |
det |
the,Database |
R1820 |
T2891 |
T2889 |
pobj |
Database,from |
R1821 |
T2892 |
T2891 |
nmod |
University,Database |
R1822 |
T2893 |
T2892 |
prep |
of,University |
R1823 |
T2894 |
T2895 |
compound |
California,Cruz |
R1824 |
T2895 |
T2893 |
pobj |
Cruz,of |
R1825 |
T2896 |
T2895 |
compound |
Santa,Cruz |
R1826 |
T2897 |
T2892 |
punct |
(,University |
R1827 |
T2898 |
T2892 |
appos |
UCSC,University |
R1828 |
T2899 |
T2891 |
punct |
),Database |
R1829 |
T2900 |
T2891 |
compound |
Genome,Database |
R1830 |
T2901 |
T2891 |
compound |
Browser,Database |
R1831 |
T2902 |
T2903 |
punct |
(,November |
R1832 |
T2903 |
T2891 |
parataxis |
November,Database |
R1833 |
T2904 |
T2903 |
punct |
;,November |
R1834 |
T2905 |
T2903 |
nmod |
October,November |
R1835 |
T2906 |
T2903 |
punct |
/,November |
R1836 |
T2907 |
T2903 |
nummod |
2004,November |
R1837 |
T2908 |
T2903 |
punct |
),November |
R1838 |
T2909 |
T2910 |
punct |
(,28 |
R1839 |
T2910 |
T2891 |
parataxis |
28,Database |
R1840 |
T2911 |
T2910 |
punct |
),28 |
R1841 |
T2912 |
T2865 |
punct |
.,obtained |
R1842 |
T2931 |
T2989 |
appos |
TTCCGATGTGCCAAGTGTGGC,Csrp1 |
R1843 |
T2932 |
T2933 |
compound |
RT,PCR |
R1844 |
T2934 |
T2933 |
punct |
–,PCR |
R1845 |
T2936 |
T2937 |
det |
All,PCRs |
R1846 |
T2937 |
T2940 |
nsubjpass |
PCRs,performed |
R1847 |
T2938 |
T2937 |
compound |
RT,PCRs |
R1848 |
T2939 |
T2937 |
punct |
–,PCRs |
R1849 |
T2941 |
T2940 |
auxpass |
were,performed |
R1850 |
T2942 |
T2940 |
prep |
with,performed |
R1851 |
T2943 |
T2944 |
compound |
QIAGEN®,Kit |
R1852 |
T2944 |
T2942 |
pobj |
Kit,with |
R1853 |
T2945 |
T2944 |
compound |
OneStep,Kit |
R1854 |
T2946 |
T2947 |
compound |
RT,PCR |
R1855 |
T2947 |
T2944 |
compound |
PCR,Kit |
R1856 |
T2948 |
T2947 |
punct |
–,PCR |
R1857 |
T2949 |
T2950 |
punct |
(,QIAGEN |
R1858 |
T2950 |
T2944 |
parataxis |
QIAGEN,Kit |
R1859 |
T2951 |
T2950 |
punct |
),QIAGEN |
R1860 |
T2952 |
T2940 |
prep |
according,performed |
R1861 |
T2953 |
T2952 |
prep |
to,according |
R1862 |
T2954 |
T2955 |
det |
the,manufacturer |
R1863 |
T2955 |
T2956 |
poss |
manufacturer,instructions |
R1864 |
T2956 |
T2953 |
pobj |
instructions,to |
R1865 |
T2957 |
T2955 |
case |
's,manufacturer |
R1866 |
T2958 |
T2940 |
punct |
", ",performed |
R1867 |
T2959 |
T2940 |
advcl |
using,performed |
R1868 |
T2960 |
T2961 |
quantmod |
150,200 |
R1869 |
T2961 |
T2963 |
nummod |
200,ng |
R1870 |
T2962 |
T2961 |
punct |
–,200 |
R1871 |
T2963 |
T2964 |
nmod |
ng,RNA |
R1872 |
T2964 |
T2959 |
dobj |
RNA,using |
R1873 |
T2965 |
T2966 |
compound |
DNase,I |
R1874 |
T2966 |
T2967 |
npadvmod |
I,digested |
R1875 |
T2967 |
T2964 |
amod |
digested,RNA |
R1876 |
T2968 |
T2967 |
punct |
-,digested |
R1877 |
T2969 |
T2964 |
amod |
total,RNA |
R1878 |
T2970 |
T2971 |
punct |
(,isolation |
R1879 |
T2971 |
T2959 |
parataxis |
isolation,using |
R1880 |
T2972 |
T2971 |
compound |
RNA,isolation |
R1881 |
T2973 |
T2971 |
advcl |
see,isolation |
R1882 |
T2974 |
T2975 |
compound |
microarray,analysis |
R1883 |
T2975 |
T2973 |
dobj |
analysis,see |
R1884 |
T2976 |
T2971 |
punct |
),isolation |
R1885 |
T2977 |
T2940 |
punct |
.,performed |
R1886 |
T2979 |
T2980 |
det |
The,reactions |
R1887 |
T2980 |
T2981 |
nsubjpass |
reactions,carried |
R1888 |
T2982 |
T2981 |
auxpass |
were,carried |
R1889 |
T2983 |
T2981 |
prt |
out,carried |
R1890 |
T2984 |
T2981 |
advcl |
using,carried |
R1891 |
T2985 |
T2986 |
det |
the,primers |
R1892 |
T2986 |
T2984 |
dobj |
primers,using |
R1893 |
T2987 |
T2986 |
amod |
following,primers |
R1894 |
T2988 |
T2986 |
punct |
: ,primers |
R1895 |
T2989 |
T2986 |
appos |
Csrp1,primers |
R1896 |
T2990 |
T2989 |
punct |
: ,Csrp1 |
R1897 |
T2991 |
T2931 |
nummod |
5,TTCCGATGTGCCAAGTGTGGC |
R1898 |
T2992 |
T2991 |
punct |
′,5 |
R1899 |
T2993 |
T2931 |
punct |
-,TTCCGATGTGCCAAGTGTGGC |
R1900 |
T2994 |
T2931 |
punct |
-,TTCCGATGTGCCAAGTGTGGC |
R1901 |
T2995 |
T2931 |
nummod |
3,TTCCGATGTGCCAAGTGTGGC |
R1902 |
T2996 |
T2931 |
punct |
′,TTCCGATGTGCCAAGTGTGGC |
R1903 |
T2997 |
T2931 |
punct |
;,TTCCGATGTGCCAAGTGTGGC |
R1904 |
T2998 |
T2999 |
nummod |
5,AGTAGAGAGTGGACATTCAGC |
R1905 |
T2999 |
T2931 |
appos |
AGTAGAGAGTGGACATTCAGC,TTCCGATGTGCCAAGTGTGGC |
R1906 |
T3000 |
T2998 |
punct |
′,5 |
R1907 |
T3001 |
T2999 |
punct |
-,AGTAGAGAGTGGACATTCAGC |
R1908 |
T3002 |
T2999 |
punct |
-,AGTAGAGAGTGGACATTCAGC |
R1909 |
T3003 |
T2999 |
nummod |
3,AGTAGAGAGTGGACATTCAGC |
R1910 |
T3004 |
T2999 |
punct |
′,AGTAGAGAGTGGACATTCAGC |
R1911 |
T3005 |
T2989 |
punct |
", ",Csrp1 |
R1912 |
T3006 |
T3007 |
compound |
hypoxanthin,phosphoribosyltransferase |
R1913 |
T3007 |
T2989 |
appos |
phosphoribosyltransferase,Csrp1 |
R1914 |
T3008 |
T3007 |
punct |
-,phosphoribosyltransferase |
R1915 |
T3009 |
T3007 |
compound |
guanin,phosphoribosyltransferase |
R1916 |
T3010 |
T3007 |
punct |
-,phosphoribosyltransferase |
R1917 |
T3011 |
T3007 |
punct |
(,phosphoribosyltransferase |
R1918 |
T3012 |
T3007 |
appos |
Hprt,phosphoribosyltransferase |
R1919 |
T3013 |
T3007 |
punct |
),phosphoribosyltransferase |
R1920 |
T3014 |
T3007 |
punct |
: ,phosphoribosyltransferase |
R1921 |
T3015 |
T3016 |
nummod |
5,GCTGGTGAAAAGGACCTCTCG |
R1922 |
T3016 |
T3007 |
appos |
GCTGGTGAAAAGGACCTCTCG,phosphoribosyltransferase |
R1923 |
T3017 |
T3015 |
punct |
′,5 |
R1924 |
T3018 |
T3016 |
punct |
-,GCTGGTGAAAAGGACCTCTCG |
R1925 |
T3019 |
T3016 |
punct |
-,GCTGGTGAAAAGGACCTCTCG |
R1926 |
T3020 |
T3016 |
nummod |
3,GCTGGTGAAAAGGACCTCTCG |
R1927 |
T3021 |
T3016 |
punct |
′,GCTGGTGAAAAGGACCTCTCG |
R1928 |
T3022 |
T3016 |
punct |
;,GCTGGTGAAAAGGACCTCTCG |
R1929 |
T3023 |
T3024 |
nummod |
5,CCACAGGACTAGGACACCTGC |
R1930 |
T3024 |
T3016 |
appos |
CCACAGGACTAGGACACCTGC,GCTGGTGAAAAGGACCTCTCG |
R1931 |
T3025 |
T3023 |
punct |
′,5 |
R1932 |
T3026 |
T3024 |
punct |
-,CCACAGGACTAGGACACCTGC |
R1933 |
T3027 |
T3024 |
punct |
-,CCACAGGACTAGGACACCTGC |
R1934 |
T3028 |
T3024 |
nummod |
3,CCACAGGACTAGGACACCTGC |
R1935 |
T3029 |
T3024 |
punct |
′,CCACAGGACTAGGACACCTGC |
R1936 |
T3030 |
T2989 |
punct |
", ",Csrp1 |
R1937 |
T3031 |
T2989 |
appos |
Mtf1,Csrp1 |
R1938 |
T3032 |
T3031 |
punct |
: ,Mtf1 |
R1939 |
T3033 |
T3034 |
nummod |
5,GTGACTTTTGAGACTGTACTGAGTG |
R1940 |
T3034 |
T3031 |
appos |
GTGACTTTTGAGACTGTACTGAGTG,Mtf1 |
R1941 |
T3035 |
T3033 |
punct |
′,5 |
R1942 |
T3036 |
T3034 |
punct |
-,GTGACTTTTGAGACTGTACTGAGTG |
R1943 |
T3037 |
T3034 |
punct |
-,GTGACTTTTGAGACTGTACTGAGTG |
R1944 |
T3038 |
T3034 |
nummod |
3,GTGACTTTTGAGACTGTACTGAGTG |
R1945 |
T3039 |
T3034 |
punct |
′,GTGACTTTTGAGACTGTACTGAGTG |
R1946 |
T3040 |
T3034 |
punct |
;,GTGACTTTTGAGACTGTACTGAGTG |
R1947 |
T3041 |
T3042 |
nummod |
5,CATGCCAAGAAACATTGAAGGTG |
R1948 |
T3042 |
T3034 |
appos |
CATGCCAAGAAACATTGAAGGTG,GTGACTTTTGAGACTGTACTGAGTG |
R1949 |
T3043 |
T3041 |
punct |
′,5 |
R1950 |
T3044 |
T3042 |
punct |
-,CATGCCAAGAAACATTGAAGGTG |
R1951 |
T3045 |
T3042 |
punct |
-,CATGCCAAGAAACATTGAAGGTG |
R1952 |
T3046 |
T3042 |
nummod |
3,CATGCCAAGAAACATTGAAGGTG |
R1953 |
T3047 |
T3042 |
punct |
′,CATGCCAAGAAACATTGAAGGTG |
R1954 |
T3048 |
T2989 |
punct |
", ",Csrp1 |
R1955 |
T3049 |
T2989 |
appos |
Ndrg1,Csrp1 |
R1956 |
T3050 |
T3049 |
punct |
: ,Ndrg1 |
R1957 |
T3051 |
T3052 |
nummod |
5,AGATACACAACAACGTGGAGG |
R1958 |
T3052 |
T3049 |
appos |
AGATACACAACAACGTGGAGG,Ndrg1 |
R1959 |
T3053 |
T3051 |
punct |
′,5 |
R1960 |
T3054 |
T3052 |
punct |
-,AGATACACAACAACGTGGAGG |
R1961 |
T3055 |
T3052 |
punct |
-,AGATACACAACAACGTGGAGG |
R1962 |
T3056 |
T3052 |
nummod |
3,AGATACACAACAACGTGGAGG |
R1963 |
T3057 |
T3052 |
punct |
′,AGATACACAACAACGTGGAGG |
R1964 |
T3058 |
T3052 |
punct |
;,AGATACACAACAACGTGGAGG |
R1965 |
T3059 |
T3060 |
nummod |
5,TGTGCGAGCGGCTTCGGGGGC |
R1966 |
T3060 |
T3052 |
appos |
TGTGCGAGCGGCTTCGGGGGC,AGATACACAACAACGTGGAGG |
R1967 |
T3061 |
T3059 |
punct |
′,5 |
R1968 |
T3062 |
T3060 |
punct |
-,TGTGCGAGCGGCTTCGGGGGC |
R1969 |
T3063 |
T3060 |
punct |
-,TGTGCGAGCGGCTTCGGGGGC |
R1970 |
T3064 |
T3060 |
nummod |
3,TGTGCGAGCGGCTTCGGGGGC |
R1971 |
T3065 |
T3060 |
punct |
′,TGTGCGAGCGGCTTCGGGGGC |
R1972 |
T3066 |
T2989 |
punct |
", ",Csrp1 |
R1973 |
T3067 |
T2989 |
appos |
Sepw1,Csrp1 |
R1974 |
T3068 |
T3067 |
punct |
: ,Sepw1 |
R1975 |
T3069 |
T3070 |
nummod |
5,TAGAGGCAGGGTCCTGAAAGC |
R1976 |
T3070 |
T3067 |
appos |
TAGAGGCAGGGTCCTGAAAGC,Sepw1 |
R1977 |
T3071 |
T3069 |
punct |
′,5 |
R1978 |
T3072 |
T3070 |
punct |
-,TAGAGGCAGGGTCCTGAAAGC |
R1979 |
T3073 |
T3070 |
punct |
-,TAGAGGCAGGGTCCTGAAAGC |
R1980 |
T3074 |
T3070 |
nummod |
3,TAGAGGCAGGGTCCTGAAAGC |
R1981 |
T3075 |
T3070 |
punct |
′,TAGAGGCAGGGTCCTGAAAGC |
R1982 |
T3076 |
T3070 |
punct |
;,TAGAGGCAGGGTCCTGAAAGC |
R1983 |
T3077 |
T3078 |
nummod |
5,ACACCTGGAAACATGGCTGCC |
R1984 |
T3078 |
T3070 |
appos |
ACACCTGGAAACATGGCTGCC,TAGAGGCAGGGTCCTGAAAGC |
R1985 |
T3079 |
T3077 |
punct |
′,5 |
R1986 |
T3080 |
T3078 |
punct |
-,ACACCTGGAAACATGGCTGCC |
R1987 |
T3081 |
T3078 |
punct |
-,ACACCTGGAAACATGGCTGCC |
R1988 |
T3082 |
T3078 |
nummod |
3,ACACCTGGAAACATGGCTGCC |
R1989 |
T3083 |
T3078 |
punct |
′,ACACCTGGAAACATGGCTGCC |
R1990 |
T3084 |
T2989 |
punct |
", ",Csrp1 |
R1991 |
T3085 |
T2989 |
appos |
Slc39a10,Csrp1 |
R1992 |
T3086 |
T3085 |
punct |
: ,Slc39a10 |
R1993 |
T3087 |
T3088 |
nummod |
5,GCTGTGGCTGGTAGTAAAAGC |
R1994 |
T3088 |
T3085 |
appos |
GCTGTGGCTGGTAGTAAAAGC,Slc39a10 |
R1995 |
T3089 |
T3087 |
punct |
′,5 |
R1996 |
T3090 |
T3088 |
punct |
-,GCTGTGGCTGGTAGTAAAAGC |
R1997 |
T3091 |
T3088 |
punct |
-,GCTGTGGCTGGTAGTAAAAGC |
R1998 |
T3092 |
T3088 |
nummod |
3,GCTGTGGCTGGTAGTAAAAGC |
R1999 |
T3093 |
T3088 |
punct |
′,GCTGTGGCTGGTAGTAAAAGC |
R2000 |
T3094 |
T3088 |
punct |
;,GCTGTGGCTGGTAGTAAAAGC |
R2001 |
T3095 |
T3096 |
nummod |
5,GTGGCATGGGATGTAAACAGC |
R2002 |
T3096 |
T3088 |
appos |
GTGGCATGGGATGTAAACAGC,GCTGTGGCTGGTAGTAAAAGC |
R2003 |
T3097 |
T3095 |
punct |
′,5 |
R2004 |
T3098 |
T3096 |
punct |
-,GTGGCATGGGATGTAAACAGC |
R2005 |
T3099 |
T3096 |
punct |
-,GTGGCATGGGATGTAAACAGC |
R2006 |
T3100 |
T3096 |
nummod |
3,GTGGCATGGGATGTAAACAGC |
R2007 |
T3101 |
T3096 |
punct |
′,GTGGCATGGGATGTAAACAGC |
R2008 |
T3102 |
T2981 |
punct |
.,carried |
R2009 |
T3137 |
T3138 |
compound |
S1,mapping |
R2010 |
T3139 |
T3138 |
compound |
nuclease,mapping |
R2011 |
T3140 |
T3138 |
prep |
of,mapping |
R2012 |
T3141 |
T3140 |
pobj |
transcripts,of |
R2013 |
T3142 |
T3143 |
punct |
(,analysis |
R2014 |
T3143 |
T3138 |
parataxis |
analysis,mapping |
R2015 |
T3144 |
T3143 |
compound |
S1,analysis |
R2016 |
T3145 |
T3143 |
punct |
),analysis |
R2017 |
T3147 |
T3148 |
compound |
S1,analysis |
R2018 |
T3148 |
T3149 |
nsubjpass |
analysis,performed |
R2019 |
T3150 |
T3149 |
auxpass |
was,performed |
R2020 |
T3151 |
T3152 |
mark |
as,described |
R2021 |
T3152 |
T3149 |
advcl |
described,performed |
R2022 |
T3153 |
T3152 |
advmod |
previously,described |
R2023 |
T3154 |
T3155 |
punct |
(,29 |
R2024 |
T3155 |
T3152 |
parataxis |
29,described |
R2025 |
T3156 |
T3155 |
punct |
),29 |
R2026 |
T3157 |
T3149 |
punct |
", ",performed |
R2027 |
T3158 |
T3149 |
advcl |
using,performed |
R2028 |
T3159 |
T3160 |
nummod |
100,µg |
R2029 |
T3160 |
T3161 |
nmod |
µg,RNA |
R2030 |
T3161 |
T3158 |
dobj |
RNA,using |
R2031 |
T3162 |
T3163 |
npadvmod |
DNase,digested |
R2032 |
T3163 |
T3161 |
amod |
digested,RNA |
R2033 |
T3164 |
T3162 |
nummod |
I,DNase |
R2034 |
T3165 |
T3163 |
punct |
-,digested |
R2035 |
T3166 |
T3161 |
amod |
total,RNA |
R2036 |
T3167 |
T3168 |
punct |
(,see |
R2037 |
T3168 |
T3161 |
parataxis |
see,RNA |
R2038 |
T3169 |
T3170 |
compound |
RNA,isolation |
R2039 |
T3170 |
T3168 |
nsubj |
isolation,see |
R2040 |
T3171 |
T3172 |
compound |
microarray,analysis |
R2041 |
T3172 |
T3168 |
dobj |
analysis,see |
R2042 |
T3173 |
T3168 |
punct |
),see |
R2043 |
T3174 |
T3149 |
punct |
.,performed |
R2044 |
T3176 |
T3177 |
det |
The,gels |
R2045 |
T3177 |
T3178 |
nsubjpass |
gels,developed |
R2046 |
T3179 |
T3178 |
auxpass |
were,developed |
R2047 |
T3180 |
T3178 |
advcl |
using,developed |
R2048 |
T3181 |
T3180 |
dobj |
PhosphorImager,using |
R2049 |
T3182 |
T3183 |
punct |
(,Dynamics |
R2050 |
T3183 |
T3178 |
parataxis |
Dynamics,developed |
R2051 |
T3184 |
T3183 |
compound |
Molecular,Dynamics |
R2052 |
T3185 |
T3183 |
punct |
),Dynamics |
R2053 |
T3186 |
T3178 |
punct |
.,developed |
R2054 |
T3188 |
T3189 |
compound |
S1,analysis |
R2055 |
T3189 |
T3190 |
nsubjpass |
analysis,done |
R2056 |
T3191 |
T3190 |
auxpass |
was,done |
R2057 |
T3192 |
T3190 |
prep |
with,done |
R2058 |
T3193 |
T3194 |
det |
the,oligonucleotides |
R2059 |
T3194 |
T3192 |
pobj |
oligonucleotides,with |
R2060 |
T3195 |
T3194 |
amod |
following,oligonucleotides |
R2061 |
T3196 |
T3197 |
npadvmod |
32P,labeled |
R2062 |
T3197 |
T3194 |
amod |
labeled,oligonucleotides |
R2063 |
T3198 |
T3197 |
punct |
-,labeled |
R2064 |
T3199 |
T3194 |
punct |
: ,oligonucleotides |
R2065 |
T3200 |
T3201 |
compound |
Hprt,S1 |
R2066 |
T3201 |
T3194 |
appos |
S1,oligonucleotides |
R2067 |
T3202 |
T3201 |
punct |
: ,S1 |
R2068 |
T3203 |
T3204 |
nummod |
5,TCTTCAGTCTGATAAAATCTACAGTCATAGGAATGGATCTATCACTATTTCTATTCAGTGATTACATTAAAG |
R2069 |
T3204 |
T3201 |
appos |
TCTTCAGTCTGATAAAATCTACAGTCATAGGAATGGATCTATCACTATTTCTATTCAGTGATTACATTAAAG,S1 |
R2070 |
T3205 |
T3203 |
punct |
′,5 |
R2071 |
T3206 |
T3204 |
punct |
-,TCTTCAGTCTGATAAAATCTACAGTCATAGGAATGGATCTATCACTATTTCTATTCAGTGATTACATTAAAG |
R2072 |
T3207 |
T3204 |
punct |
-,TCTTCAGTCTGATAAAATCTACAGTCATAGGAATGGATCTATCACTATTTCTATTCAGTGATTACATTAAAG |
R2073 |
T3208 |
T3204 |
nummod |
3,TCTTCAGTCTGATAAAATCTACAGTCATAGGAATGGATCTATCACTATTTCTATTCAGTGATTACATTAAAG |
R2074 |
T3209 |
T3204 |
punct |
′,TCTTCAGTCTGATAAAATCTACAGTCATAGGAATGGATCTATCACTATTTCTATTCAGTGATTACATTAAAG |
R2075 |
T3210 |
T3201 |
punct |
", ",S1 |
R2076 |
T3211 |
T3212 |
compound |
Sepw1,S1 |
R2077 |
T3212 |
T3201 |
appos |
S1,S1 |
R2078 |
T3213 |
T3212 |
punct |
: ,S1 |
R2079 |
T3214 |
T3215 |
nummod |
5,TTCAACCGGGAACACCTGGAAACATGGCTGCCTGTCTTCTTGAAGTCTTGAGGTGGAAAGGGAAAGCAAAGCAGGAGGGTTTTCCCACCC |
R2080 |
T3215 |
T3212 |
appos |
TTCAACCGGGAACACCTGGAAACATGGCTGCCTGTCTTCTTGAAGTCTTGAGGTGGAAAGGGAAAGCAAAGCAGGAGGGTTTTCCCACCC,S1 |
R2081 |
T3216 |
T3214 |
punct |
′,5 |
R2082 |
T3217 |
T3215 |
punct |
-,TTCAACCGGGAACACCTGGAAACATGGCTGCCTGTCTTCTTGAAGTCTTGAGGTGGAAAGGGAAAGCAAAGCAGGAGGGTTTTCCCACCC |
R2083 |
T3218 |
T3215 |
punct |
-,TTCAACCGGGAACACCTGGAAACATGGCTGCCTGTCTTCTTGAAGTCTTGAGGTGGAAAGGGAAAGCAAAGCAGGAGGGTTTTCCCACCC |
R2084 |
T3219 |
T3215 |
nummod |
3,TTCAACCGGGAACACCTGGAAACATGGCTGCCTGTCTTCTTGAAGTCTTGAGGTGGAAAGGGAAAGCAAAGCAGGAGGGTTTTCCCACCC |
R2085 |
T3220 |
T3215 |
punct |
′,TTCAACCGGGAACACCTGGAAACATGGCTGCCTGTCTTCTTGAAGTCTTGAGGTGGAAAGGGAAAGCAAAGCAGGAGGGTTTTCCCACCC |
R2086 |
T3221 |
T3190 |
punct |
.,done |
R2089 |
T3351 |
T3352 |
amod |
Electrophoretic,shift |
R2090 |
T3352 |
T3354 |
compound |
shift,assay |
R2091 |
T3353 |
T3352 |
compound |
mobility,shift |
R2092 |
T3355 |
T3354 |
punct |
(,assay |
R2093 |
T3356 |
T3354 |
appos |
EMSA,assay |
R2094 |
T3357 |
T3354 |
punct |
),assay |
R2095 |
T3359 |
T3360 |
nsubjpass |
Protein,extracted |
R2096 |
T3361 |
T3360 |
auxpass |
was,extracted |
R2097 |
T3362 |
T3360 |
prep |
from,extracted |
R2098 |
T3363 |
T3364 |
compound |
liver,tissue |
R2099 |
T3364 |
T3362 |
pobj |
tissue,from |
R2100 |
T3365 |
T3360 |
prep |
with,extracted |
R2101 |
T3366 |
T3367 |
compound |
T,PER |
R2102 |
T3367 |
T3365 |
pobj |
PER,with |
R2103 |
T3368 |
T3367 |
punct |
-,PER |
R2104 |
T3369 |
T3367 |
punct |
™,PER |
R2105 |
T3370 |
T3371 |
compound |
Tissue,Protein |
R2106 |
T3371 |
T3372 |
compound |
Protein,Extraction |
R2107 |
T3372 |
T3373 |
compound |
Extraction,Reagent |
R2108 |
T3373 |
T3367 |
appos |
Reagent,PER |
R2109 |
T3374 |
T3375 |
punct |
(,Pierce |
R2110 |
T3375 |
T3367 |
parataxis |
Pierce,PER |
R2111 |
T3376 |
T3375 |
punct |
),Pierce |
R2112 |
T3377 |
T3360 |
prep |
according,extracted |
R2113 |
T3378 |
T3377 |
prep |
to,according |
R2114 |
T3379 |
T3380 |
det |
the,manufacturer |
R2115 |
T3380 |
T3381 |
poss |
manufacturer,instructions |
R2116 |
T3381 |
T3378 |
pobj |
instructions,to |
R2117 |
T3382 |
T3380 |
case |
's,manufacturer |
R2118 |
T3383 |
T3360 |
punct |
", ",extracted |
R2119 |
T3384 |
T3360 |
advcl |
using,extracted |
R2120 |
T3385 |
T3386 |
det |
a,ratio |
R2121 |
T3386 |
T3384 |
dobj |
ratio,using |
R2122 |
T3387 |
T3386 |
nummod |
1,ratio |
R2123 |
T3388 |
T3389 |
punct |
:,10 |
R2124 |
T3389 |
T3387 |
prep |
10,1 |
R2125 |
T3390 |
T3386 |
prep |
of,ratio |
R2126 |
T3391 |
T3392 |
compound |
mouse,tissue |
R2127 |
T3392 |
T3390 |
pobj |
tissue,of |
R2128 |
T3393 |
T3394 |
punct |
(,mg |
R2129 |
T3394 |
T3392 |
parataxis |
mg,tissue |
R2130 |
T3395 |
T3394 |
punct |
),mg |
R2131 |
T3396 |
T3386 |
prep |
to,ratio |
R2132 |
T3397 |
T3398 |
compound |
T,PER |
R2133 |
T3398 |
T3400 |
compound |
PER,reagent |
R2134 |
T3399 |
T3398 |
punct |
-,PER |
R2135 |
T3400 |
T3396 |
pobj |
reagent,to |
R2136 |
T3401 |
T3402 |
punct |
(,µl |
R2137 |
T3402 |
T3400 |
parataxis |
µl,reagent |
R2138 |
T3403 |
T3402 |
punct |
),µl |
R2139 |
T3404 |
T3360 |
punct |
.,extracted |
R2140 |
T3406 |
T3407 |
nsubjpass |
EMSA,performed |
R2141 |
T3408 |
T3407 |
auxpass |
was,performed |
R2142 |
T3409 |
T3407 |
advmod |
essentially,performed |
R2143 |
T3410 |
T3411 |
mark |
as,described |
R2144 |
T3411 |
T3407 |
advcl |
described,performed |
R2145 |
T3412 |
T3411 |
advmod |
previously,described |
R2146 |
T3413 |
T3414 |
punct |
(,10 |
R2147 |
T3414 |
T3411 |
parataxis |
10,described |
R2148 |
T3415 |
T3414 |
punct |
),10 |
R2149 |
T3416 |
T3407 |
punct |
.,performed |
R2150 |
T3418 |
T3419 |
det |
All,reactions |
R2151 |
T3419 |
T3421 |
nsubjpass |
reactions,carried |
R2152 |
T3420 |
T3419 |
compound |
binding,reactions |
R2153 |
T3422 |
T3421 |
auxpass |
were,carried |
R2154 |
T3423 |
T3421 |
prt |
out,carried |
R2155 |
T3424 |
T3421 |
prep |
by,carried |
R2156 |
T3425 |
T3424 |
pcomp |
incubating,by |
R2157 |
T3426 |
T3427 |
quantmod |
2,5 |
R2158 |
T3427 |
T3429 |
nummod |
5,fmol |
R2159 |
T3428 |
T3427 |
punct |
–,5 |
R2160 |
T3429 |
T3430 |
nmod |
fmol,oligonucleotides |
R2162 |
T3431 |
T3432 |
npadvmod |
32P,labeled |
R2163 |
T3432 |
T3430 |
amod |
labeled,oligonucleotides |
R2164 |
T3433 |
T3432 |
punct |
-,labeled |
R2165 |
T3434 |
T3432 |
npadvmod |
end,labeled |
R2166 |
T3435 |
T3432 |
punct |
-,labeled |
R2167 |
T3436 |
T3437 |
amod |
double,stranded |
R2168 |
T3437 |
T3430 |
amod |
stranded,oligonucleotides |
R2169 |
T3438 |
T3437 |
punct |
-,stranded |
R2170 |
T3439 |
T3425 |
prep |
with,incubating |
R2171 |
T3440 |
T3441 |
quantmod |
100,130 |
R2172 |
T3441 |
T3443 |
nummod |
130,µg |
R2173 |
T3442 |
T3441 |
quantmod |
to,130 |
R2174 |
T3443 |
T3444 |
compound |
µg,extract |
R2175 |
T3444 |
T3439 |
pobj |
extract,with |
R2176 |
T3445 |
T3446 |
compound |
liver,protein |
R2177 |
T3446 |
T3444 |
compound |
protein,extract |
R2178 |
T3447 |
T3421 |
punct |
.,carried |
R2179 |
T3449 |
T3450 |
prep |
For,added |
R2180 |
T3451 |
T3452 |
compound |
competition,experiments |
R2181 |
T3452 |
T3449 |
pobj |
experiments,For |
R2182 |
T3453 |
T3450 |
punct |
", ",added |
R2183 |
T3454 |
T3455 |
nummod |
5,pmol |
R2184 |
T3455 |
T3450 |
nsubjpass |
pmol,added |
R2185 |
T3456 |
T3455 |
prep |
of,pmol |
R2186 |
T3457 |
T3458 |
amod |
unlabeled,oligonucleotide |
R2187 |
T3458 |
T3456 |
pobj |
oligonucleotide,of |
R2188 |
T3459 |
T3458 |
compound |
competitor,oligonucleotide |
R2189 |
T3460 |
T3450 |
auxpass |
was,added |
R2190 |
T3461 |
T3450 |
prep |
to,added |
R2191 |
T3462 |
T3463 |
det |
the,reaction |
R2192 |
T3463 |
T3461 |
pobj |
reaction,to |
R2193 |
T3464 |
T3463 |
compound |
binding,reaction |
R2194 |
T3465 |
T3450 |
prep |
before,added |
R2195 |
T3466 |
T3465 |
pobj |
addition,before |
R2196 |
T3467 |
T3466 |
prep |
of,addition |
R2197 |
T3468 |
T3469 |
det |
the,extract |
R2198 |
T3469 |
T3467 |
pobj |
extract,of |
R2199 |
T3470 |
T3450 |
punct |
.,added |
R2200 |
T3472 |
T3473 |
det |
All,gels |
R2201 |
T3473 |
T3475 |
nsubjpass |
gels,developed |
R2202 |
T3474 |
T3473 |
compound |
EMSA,gels |
R2203 |
T3476 |
T3475 |
auxpass |
were,developed |
R2204 |
T3477 |
T3475 |
advcl |
using,developed |
R2205 |
T3478 |
T3477 |
dobj |
PhosphorImager,using |
R2206 |
T3479 |
T3480 |
punct |
(,Dynamics |
R2207 |
T3480 |
T3478 |
parataxis |
Dynamics,PhosphorImager |
R2208 |
T3481 |
T3480 |
compound |
Molecular,Dynamics |
R2209 |
T3482 |
T3480 |
punct |
),Dynamics |
R2210 |
T3483 |
T3475 |
punct |
.,developed |
R2211 |
T3485 |
T3486 |
det |
The,oligonucleotides |
R2212 |
T3486 |
T3488 |
nsubjpass |
oligonucleotides,annealed |
R2213 |
T3487 |
T3486 |
amod |
following,oligonucleotides |
R2214 |
T3489 |
T3488 |
auxpass |
were,annealed |
R2215 |
T3490 |
T3488 |
cc |
and,annealed |
R2216 |
T3491 |
T3488 |
conj |
used,annealed |
R2217 |
T3492 |
T3491 |
prep |
for,used |
R2218 |
T3493 |
T3494 |
det |
the,reactions |
R2219 |
T3494 |
T3492 |
pobj |
reactions,for |
R2220 |
T3495 |
T3491 |
punct |
: ,used |
R2221 |
T3496 |
T3497 |
compound |
Csrp1,MRE1 |
R2222 |
T3497 |
T3491 |
dobj |
MRE1,used |
R2223 |
T3498 |
T3497 |
punct |
: ,MRE1 |
R2224 |
T3499 |
T3500 |
nummod |
5,GGAAACAAAACGGCGCGCACTCCGGCGC |
R2225 |
T3500 |
T3497 |
appos |
GGAAACAAAACGGCGCGCACTCCGGCGC,MRE1 |
R2226 |
T3501 |
T3499 |
punct |
′,5 |
R2227 |
T3502 |
T3500 |
punct |
-,GGAAACAAAACGGCGCGCACTCCGGCGC |
R2228 |
T3503 |
T3500 |
punct |
-,GGAAACAAAACGGCGCGCACTCCGGCGC |
R2229 |
T3504 |
T3500 |
nummod |
3,GGAAACAAAACGGCGCGCACTCCGGCGC |
R2230 |
T3505 |
T3500 |
punct |
′,GGAAACAAAACGGCGCGCACTCCGGCGC |
R2231 |
T3506 |
T3500 |
punct |
;,GGAAACAAAACGGCGCGCACTCCGGCGC |
R2232 |
T3507 |
T3508 |
nummod |
5,CGGAGTGCGCGCCGTTTTGT |
R2233 |
T3508 |
T3500 |
appos |
CGGAGTGCGCGCCGTTTTGT,GGAAACAAAACGGCGCGCACTCCGGCGC |
R2234 |
T3509 |
T3507 |
punct |
′,5 |
R2235 |
T3510 |
T3508 |
punct |
-,CGGAGTGCGCGCCGTTTTGT |
R2236 |
T3511 |
T3508 |
compound |
GGCTGCGC,CGGAGTGCGCGCCGTTTTGT |
R2237 |
T3512 |
T3508 |
punct |
-,CGGAGTGCGCGCCGTTTTGT |
R2238 |
T3513 |
T3508 |
nummod |
3,CGGAGTGCGCGCCGTTTTGT |
R2239 |
T3514 |
T3508 |
punct |
′,CGGAGTGCGCGCCGTTTTGT |
R2240 |
T3515 |
T3497 |
punct |
", ",MRE1 |
R2241 |
T3516 |
T3517 |
compound |
Csrp1,MRE2 |
R2242 |
T3517 |
T3497 |
appos |
MRE2,MRE1 |
R2243 |
T3518 |
T3517 |
punct |
: ,MRE2 |
R2244 |
T3519 |
T3520 |
nummod |
5,TGTTGTGGTGCAGTGTGCAAAGCCTAC |
R2245 |
T3520 |
T3517 |
appos |
TGTTGTGGTGCAGTGTGCAAAGCCTAC,MRE2 |
R2246 |
T3521 |
T3519 |
punct |
′,5 |
R2247 |
T3522 |
T3520 |
punct |
-,TGTTGTGGTGCAGTGTGCAAAGCCTAC |
R2248 |
T3523 |
T3520 |
punct |
-,TGTTGTGGTGCAGTGTGCAAAGCCTAC |
R2249 |
T3524 |
T3520 |
nummod |
3,TGTTGTGGTGCAGTGTGCAAAGCCTAC |
R2250 |
T3525 |
T3520 |
punct |
′,TGTTGTGGTGCAGTGTGCAAAGCCTAC |
R2251 |
T3526 |
T3520 |
punct |
;,TGTTGTGGTGCAGTGTGCAAAGCCTAC |
R2252 |
T3527 |
T3528 |
nummod |
5,ACCAGTAGGCTTTGCACACTGCACCAC |
R2253 |
T3528 |
T3520 |
appos |
ACCAGTAGGCTTTGCACACTGCACCAC,TGTTGTGGTGCAGTGTGCAAAGCCTAC |
R2254 |
T3529 |
T3527 |
punct |
′,5 |
R2255 |
T3530 |
T3528 |
punct |
-,ACCAGTAGGCTTTGCACACTGCACCAC |
R2256 |
T3531 |
T3528 |
punct |
-,ACCAGTAGGCTTTGCACACTGCACCAC |
R2257 |
T3532 |
T3528 |
nummod |
3,ACCAGTAGGCTTTGCACACTGCACCAC |
R2258 |
T3533 |
T3528 |
punct |
′,ACCAGTAGGCTTTGCACACTGCACCAC |
R2259 |
T3534 |
T3497 |
punct |
", ",MRE1 |
R2260 |
T3535 |
T3536 |
compound |
Csrp1,MRE3 |
R2261 |
T3536 |
T3497 |
appos |
MRE3,MRE1 |
R2262 |
T3537 |
T3536 |
punct |
: ,MRE3 |
R2263 |
T3538 |
T3539 |
nummod |
5,GAGATCGCCATAGGGTGCAAAGAGAAG |
R2264 |
T3539 |
T3536 |
appos |
GAGATCGCCATAGGGTGCAAAGAGAAG,MRE3 |
R2265 |
T3540 |
T3538 |
punct |
′,5 |
R2266 |
T3541 |
T3539 |
punct |
-,GAGATCGCCATAGGGTGCAAAGAGAAG |
R2267 |
T3542 |
T3539 |
punct |
-,GAGATCGCCATAGGGTGCAAAGAGAAG |
R2268 |
T3543 |
T3539 |
nummod |
3,GAGATCGCCATAGGGTGCAAAGAGAAG |
R2269 |
T3544 |
T3539 |
punct |
′,GAGATCGCCATAGGGTGCAAAGAGAAG |
R2270 |
T3545 |
T3539 |
punct |
;,GAGATCGCCATAGGGTGCAAAGAGAAG |
R2271 |
T3546 |
T3547 |
nummod |
5,GTGACTTCTCTTTGCACCCTATGGCGA |
R2272 |
T3547 |
T3539 |
appos |
GTGACTTCTCTTTGCACCCTATGGCGA,GAGATCGCCATAGGGTGCAAAGAGAAG |
R2273 |
T3548 |
T3546 |
punct |
′,5 |
R2274 |
T3549 |
T3547 |
punct |
-,GTGACTTCTCTTTGCACCCTATGGCGA |
R2275 |
T3550 |
T3547 |
punct |
-,GTGACTTCTCTTTGCACCCTATGGCGA |
R2276 |
T3551 |
T3547 |
nummod |
3,GTGACTTCTCTTTGCACCCTATGGCGA |
R2277 |
T3552 |
T3547 |
punct |
′,GTGACTTCTCTTTGCACCCTATGGCGA |
R2278 |
T3553 |
T3497 |
punct |
", ",MRE1 |
R2279 |
T3554 |
T3555 |
compound |
Csrp1,MRE4 |
R2280 |
T3555 |
T3497 |
appos |
MRE4,MRE1 |
R2281 |
T3556 |
T3555 |
punct |
: ,MRE4 |
R2282 |
T3557 |
T3558 |
nummod |
5,TGTCTTATTCTGGAGTGCAAGTTAGTC |
R2283 |
T3558 |
T3555 |
appos |
TGTCTTATTCTGGAGTGCAAGTTAGTC,MRE4 |
R2284 |
T3559 |
T3557 |
punct |
′,5 |
R2285 |
T3560 |
T3558 |
punct |
-,TGTCTTATTCTGGAGTGCAAGTTAGTC |
R2286 |
T3561 |
T3558 |
punct |
-,TGTCTTATTCTGGAGTGCAAGTTAGTC |
R2287 |
T3562 |
T3558 |
nummod |
3,TGTCTTATTCTGGAGTGCAAGTTAGTC |
R2288 |
T3563 |
T3558 |
punct |
′,TGTCTTATTCTGGAGTGCAAGTTAGTC |
R2289 |
T3564 |
T3558 |
punct |
;,TGTCTTATTCTGGAGTGCAAGTTAGTC |
R2290 |
T3565 |
T3566 |
nummod |
5,AGGGGACTAACTTGCACTCCAGAATAA |
R2291 |
T3566 |
T3558 |
appos |
AGGGGACTAACTTGCACTCCAGAATAA,TGTCTTATTCTGGAGTGCAAGTTAGTC |
R2292 |
T3567 |
T3565 |
punct |
′,5 |
R2293 |
T3568 |
T3566 |
punct |
-,AGGGGACTAACTTGCACTCCAGAATAA |
R2294 |
T3569 |
T3566 |
punct |
-,AGGGGACTAACTTGCACTCCAGAATAA |
R2295 |
T3570 |
T3566 |
nummod |
3,AGGGGACTAACTTGCACTCCAGAATAA |
R2296 |
T3571 |
T3566 |
punct |
′,AGGGGACTAACTTGCACTCCAGAATAA |
R2297 |
T3572 |
T3497 |
punct |
", ",MRE1 |
R2298 |
T3573 |
T3497 |
appos |
Gal4,MRE1 |
R2299 |
T3574 |
T3573 |
punct |
: ,Gal4 |
R2300 |
T3575 |
T3576 |
nummod |
5,TCCGGAGGACTGTCCTCCGG |
R2301 |
T3576 |
T3573 |
appos |
TCCGGAGGACTGTCCTCCGG,Gal4 |
R2302 |
T3577 |
T3575 |
punct |
′,5 |
R2303 |
T3578 |
T3576 |
punct |
-,TCCGGAGGACTGTCCTCCGG |
R2304 |
T3579 |
T3576 |
punct |
-,TCCGGAGGACTGTCCTCCGG |
R2305 |
T3580 |
T3576 |
nummod |
3,TCCGGAGGACTGTCCTCCGG |
R2306 |
T3581 |
T3576 |
punct |
′,TCCGGAGGACTGTCCTCCGG |
R2307 |
T3582 |
T3576 |
punct |
;,TCCGGAGGACTGTCCTCCGG |
R2308 |
T3583 |
T3584 |
nummod |
5,GCCGGAGGACAGTCCTCCGG |
R2309 |
T3584 |
T3576 |
appos |
GCCGGAGGACAGTCCTCCGG,TCCGGAGGACTGTCCTCCGG |
R2310 |
T3585 |
T3583 |
punct |
′,5 |
R2311 |
T3586 |
T3584 |
punct |
-,GCCGGAGGACAGTCCTCCGG |
R2312 |
T3587 |
T3584 |
punct |
-,GCCGGAGGACAGTCCTCCGG |
R2313 |
T3588 |
T3584 |
nummod |
3,GCCGGAGGACAGTCCTCCGG |
R2314 |
T3589 |
T3584 |
punct |
′,GCCGGAGGACAGTCCTCCGG |
R2315 |
T3590 |
T3497 |
punct |
", ",MRE1 |
R2316 |
T3591 |
T3497 |
appos |
MREd,MRE1 |
R2317 |
T3592 |
T3591 |
punct |
[,MREd |
R2318 |
T3593 |
T3591 |
appos |
MRE,MREd |
R2319 |
T3594 |
T3593 |
acl |
derived,MRE |
R2320 |
T3595 |
T3594 |
prep |
from,derived |
R2321 |
T3596 |
T3597 |
compound |
mouse,promoter |
R2322 |
T3597 |
T3595 |
pobj |
promoter,from |
R2323 |
T3598 |
T3597 |
compound |
Mt1,promoter |
R2324 |
T3599 |
T3600 |
punct |
(,10 |
R2325 |
T3600 |
T3594 |
parataxis |
10,derived |
R2326 |
T3601 |
T3600 |
punct |
),10 |
R2327 |
T3602 |
T3591 |
punct |
],MREd |
R2328 |
T3603 |
T3591 |
punct |
: ,MREd |
R2329 |
T3604 |
T3605 |
nummod |
5,CGAGGGAGCTCTGCACTCCGCCCGAAAAGTG |
R2330 |
T3605 |
T3591 |
appos |
CGAGGGAGCTCTGCACTCCGCCCGAAAAGTG,MREd |
R2331 |
T3606 |
T3604 |
punct |
′,5 |
R2332 |
T3607 |
T3605 |
punct |
-,CGAGGGAGCTCTGCACTCCGCCCGAAAAGTG |
R2333 |
T3608 |
T3605 |
punct |
-,CGAGGGAGCTCTGCACTCCGCCCGAAAAGTG |
R2334 |
T3609 |
T3605 |
nummod |
3,CGAGGGAGCTCTGCACTCCGCCCGAAAAGTG |
R2335 |
T3610 |
T3605 |
punct |
′,CGAGGGAGCTCTGCACTCCGCCCGAAAAGTG |
R2336 |
T3611 |
T3605 |
punct |
;,CGAGGGAGCTCTGCACTCCGCCCGAAAAGTG |
R2337 |
T3612 |
T3613 |
nummod |
5,TCGACACTTTTCGGGCGGAGTGCAGAGCTCCCTCGAGCT |
R2338 |
T3613 |
T3605 |
appos |
TCGACACTTTTCGGGCGGAGTGCAGAGCTCCCTCGAGCT,CGAGGGAGCTCTGCACTCCGCCCGAAAAGTG |
R2339 |
T3614 |
T3612 |
punct |
′,5 |
R2340 |
T3615 |
T3613 |
punct |
-,TCGACACTTTTCGGGCGGAGTGCAGAGCTCCCTCGAGCT |
R2341 |
T3616 |
T3613 |
punct |
-,TCGACACTTTTCGGGCGGAGTGCAGAGCTCCCTCGAGCT |
R2342 |
T3617 |
T3613 |
nummod |
3,TCGACACTTTTCGGGCGGAGTGCAGAGCTCCCTCGAGCT |
R2343 |
T3618 |
T3613 |
punct |
′,TCGACACTTTTCGGGCGGAGTGCAGAGCTCCCTCGAGCT |
R2344 |
T3619 |
T3497 |
punct |
", ",MRE1 |
R2345 |
T3620 |
T3621 |
compound |
MRE,s |
R2346 |
T3621 |
T3497 |
appos |
s,MRE1 |
R2347 |
T3622 |
T3621 |
punct |
-,s |
R2348 |
T3623 |
T3621 |
punct |
[,s |
R2349 |
T3624 |
T3625 |
amod |
synthetic,sequence |
R2350 |
T3625 |
T3621 |
appos |
sequence,s |
R2351 |
T3626 |
T3625 |
compound |
MRE,sequence |
R2352 |
T3627 |
T3625 |
compound |
consensus,sequence |
R2353 |
T3628 |
T3629 |
punct |
(,10 |
R2354 |
T3629 |
T3625 |
parataxis |
10,sequence |
R2355 |
T3630 |
T3629 |
punct |
),10 |
R2356 |
T3631 |
T3621 |
punct |
],s |
R2357 |
T3632 |
T3621 |
punct |
: ,s |
R2358 |
T3633 |
T3634 |
nummod |
5,CGAGGGAGCTCTGCACACGGCCCGAAAAGTG |
R2359 |
T3634 |
T3621 |
appos |
CGAGGGAGCTCTGCACACGGCCCGAAAAGTG,s |
R2360 |
T3635 |
T3633 |
punct |
′,5 |
R2361 |
T3636 |
T3634 |
punct |
-,CGAGGGAGCTCTGCACACGGCCCGAAAAGTG |
R2362 |
T3637 |
T3634 |
punct |
-,CGAGGGAGCTCTGCACACGGCCCGAAAAGTG |
R2363 |
T3638 |
T3634 |
nummod |
3,CGAGGGAGCTCTGCACACGGCCCGAAAAGTG |
R2364 |
T3639 |
T3634 |
punct |
′,CGAGGGAGCTCTGCACACGGCCCGAAAAGTG |
R2365 |
T3640 |
T3634 |
punct |
;,CGAGGGAGCTCTGCACACGGCCCGAAAAGTG |
R2366 |
T3641 |
T3642 |
nummod |
5,TCGACACTTTTCGGGCCGTGTGCAGAGCTCCCTCGAGCT |
R2367 |
T3642 |
T3634 |
appos |
TCGACACTTTTCGGGCCGTGTGCAGAGCTCCCTCGAGCT,CGAGGGAGCTCTGCACACGGCCCGAAAAGTG |
R2368 |
T3643 |
T3641 |
punct |
′,5 |
R2369 |
T3644 |
T3642 |
punct |
-,TCGACACTTTTCGGGCCGTGTGCAGAGCTCCCTCGAGCT |
R2370 |
T3645 |
T3642 |
punct |
-,TCGACACTTTTCGGGCCGTGTGCAGAGCTCCCTCGAGCT |
R2371 |
T3646 |
T3642 |
nummod |
3,TCGACACTTTTCGGGCCGTGTGCAGAGCTCCCTCGAGCT |
R2372 |
T3647 |
T3642 |
punct |
′,TCGACACTTTTCGGGCCGTGTGCAGAGCTCCCTCGAGCT |
R2373 |
T3648 |
T3497 |
punct |
", ",MRE1 |
R2374 |
T3649 |
T3650 |
compound |
Ndrg1,MRE1 |
R2375 |
T3650 |
T3497 |
appos |
MRE1,MRE1 |
R2376 |
T3651 |
T3650 |
punct |
: ,MRE1 |
R2377 |
T3652 |
T3653 |
nummod |
5,CAGCCCAGGCAGGGTGCAGCACGAG |
R2378 |
T3653 |
T3650 |
appos |
CAGCCCAGGCAGGGTGCAGCACGAG,MRE1 |
R2379 |
T3654 |
T3652 |
punct |
′,5 |
R2380 |
T3655 |
T3653 |
punct |
-,CAGCCCAGGCAGGGTGCAGCACGAG |
R2381 |
T3656 |
T3653 |
punct |
-,CAGCCCAGGCAGGGTGCAGCACGAG |
R2382 |
T3657 |
T3653 |
nummod |
3,CAGCCCAGGCAGGGTGCAGCACGAG |
R2383 |
T3658 |
T3653 |
punct |
′,CAGCCCAGGCAGGGTGCAGCACGAG |
R2384 |
T3659 |
T3653 |
punct |
;,CAGCCCAGGCAGGGTGCAGCACGAG |
R2385 |
T3660 |
T3661 |
nummod |
5,CCGCCTCGTGCTGCACCCTGCCTGG |
R2386 |
T3661 |
T3653 |
appos |
CCGCCTCGTGCTGCACCCTGCCTGG,CAGCCCAGGCAGGGTGCAGCACGAG |
R2387 |
T3662 |
T3660 |
punct |
′,5 |
R2388 |
T3663 |
T3661 |
punct |
-,CCGCCTCGTGCTGCACCCTGCCTGG |
R2389 |
T3664 |
T3661 |
punct |
-,CCGCCTCGTGCTGCACCCTGCCTGG |
R2390 |
T3665 |
T3661 |
nummod |
3,CCGCCTCGTGCTGCACCCTGCCTGG |
R2391 |
T3666 |
T3661 |
punct |
′,CCGCCTCGTGCTGCACCCTGCCTGG |
R2392 |
T3667 |
T3497 |
punct |
", ",MRE1 |
R2393 |
T3668 |
T3669 |
compound |
Ndrg1,MRE2 |
R2394 |
T3669 |
T3497 |
appos |
MRE2,MRE1 |
R2395 |
T3670 |
T3669 |
punct |
: ,MRE2 |
R2396 |
T3671 |
T3672 |
nummod |
5,CACACGTTCGCTGCACACGCCGCGG |
R2397 |
T3672 |
T3669 |
appos |
CACACGTTCGCTGCACACGCCGCGG,MRE2 |
R2398 |
T3673 |
T3671 |
punct |
′,5 |
R2399 |
T3674 |
T3672 |
punct |
-,CACACGTTCGCTGCACACGCCGCGG |
R2400 |
T3675 |
T3672 |
punct |
-,CACACGTTCGCTGCACACGCCGCGG |
R2401 |
T3676 |
T3672 |
nummod |
3,CACACGTTCGCTGCACACGCCGCGG |
R2402 |
T3677 |
T3672 |
punct |
′,CACACGTTCGCTGCACACGCCGCGG |
R2403 |
T3678 |
T3672 |
punct |
;,CACACGTTCGCTGCACACGCCGCGG |
R2404 |
T3679 |
T3680 |
nummod |
5,GGGACCGCGGCGTGTGCAGCGAACG |
R2405 |
T3680 |
T3672 |
appos |
GGGACCGCGGCGTGTGCAGCGAACG,CACACGTTCGCTGCACACGCCGCGG |
R2406 |
T3681 |
T3679 |
punct |
′,5 |
R2407 |
T3682 |
T3680 |
punct |
-,GGGACCGCGGCGTGTGCAGCGAACG |
R2408 |
T3683 |
T3680 |
punct |
-,GGGACCGCGGCGTGTGCAGCGAACG |
R2409 |
T3684 |
T3680 |
nummod |
3,GGGACCGCGGCGTGTGCAGCGAACG |
R2410 |
T3685 |
T3680 |
punct |
′,GGGACCGCGGCGTGTGCAGCGAACG |
R2411 |
T3686 |
T3497 |
punct |
", ",MRE1 |
R2412 |
T3687 |
T3688 |
compound |
Ndrg1,MRE3 |
R2413 |
T3688 |
T3497 |
appos |
MRE3,MRE1 |
R2414 |
T3689 |
T3688 |
punct |
",",MRE3 |
R2415 |
T3690 |
T3688 |
nummod |
4,MRE3 |
R2416 |
T3691 |
T3688 |
punct |
: ,MRE3 |
R2417 |
T3692 |
T3693 |
nummod |
5,GGAGTCCTTATGCACACGCGCACGAGCGCGCACGGGCAC |
R2418 |
T3693 |
T3688 |
appos |
GGAGTCCTTATGCACACGCGCACGAGCGCGCACGGGCAC,MRE3 |
R2419 |
T3694 |
T3692 |
punct |
′,5 |
R2420 |
T3695 |
T3693 |
punct |
-,GGAGTCCTTATGCACACGCGCACGAGCGCGCACGGGCAC |
R2421 |
T3696 |
T3693 |
punct |
-,GGAGTCCTTATGCACACGCGCACGAGCGCGCACGGGCAC |
R2422 |
T3697 |
T3693 |
nummod |
3,GGAGTCCTTATGCACACGCGCACGAGCGCGCACGGGCAC |
R2423 |
T3698 |
T3693 |
punct |
′,GGAGTCCTTATGCACACGCGCACGAGCGCGCACGGGCAC |
R2424 |
T3699 |
T3693 |
punct |
;,GGAGTCCTTATGCACACGCGCACGAGCGCGCACGGGCAC |
R2425 |
T3700 |
T3701 |
nummod |
5,TGGTGTGCCCGTGCGCGCTCGTGCGCGTGTGCATAAGGAC |
R2426 |
T3701 |
T3693 |
appos |
TGGTGTGCCCGTGCGCGCTCGTGCGCGTGTGCATAAGGAC,GGAGTCCTTATGCACACGCGCACGAGCGCGCACGGGCAC |
R2427 |
T3702 |
T3700 |
punct |
′,5 |
R2428 |
T3703 |
T3701 |
punct |
-,TGGTGTGCCCGTGCGCGCTCGTGCGCGTGTGCATAAGGAC |
R2429 |
T3704 |
T3701 |
punct |
-,TGGTGTGCCCGTGCGCGCTCGTGCGCGTGTGCATAAGGAC |
R2430 |
T3705 |
T3701 |
nummod |
3,TGGTGTGCCCGTGCGCGCTCGTGCGCGTGTGCATAAGGAC |
R2431 |
T3706 |
T3701 |
punct |
′,TGGTGTGCCCGTGCGCGCTCGTGCGCGTGTGCATAAGGAC |
R2432 |
T3707 |
T3497 |
punct |
", ",MRE1 |
R2433 |
T3708 |
T3709 |
compound |
Sepw1,MRE1 |
R2434 |
T3709 |
T3497 |
appos |
MRE1,MRE1 |
R2435 |
T3710 |
T3709 |
punct |
: ,MRE1 |
R2436 |
T3711 |
T3712 |
nummod |
5,GAGGCAGTCGGCTGTGCGCACGGCCCCACGCTC |
R2437 |
T3712 |
T3709 |
appos |
GAGGCAGTCGGCTGTGCGCACGGCCCCACGCTC,MRE1 |
R2438 |
T3713 |
T3711 |
punct |
′,5 |
R2439 |
T3714 |
T3712 |
punct |
-,GAGGCAGTCGGCTGTGCGCACGGCCCCACGCTC |
R2440 |
T3715 |
T3712 |
punct |
-,GAGGCAGTCGGCTGTGCGCACGGCCCCACGCTC |
R2441 |
T3716 |
T3712 |
nummod |
3,GAGGCAGTCGGCTGTGCGCACGGCCCCACGCTC |
R2442 |
T3717 |
T3712 |
punct |
′,GAGGCAGTCGGCTGTGCGCACGGCCCCACGCTC |
R2443 |
T3718 |
T3712 |
punct |
;,GAGGCAGTCGGCTGTGCGCACGGCCCCACGCTC |
R2444 |
T3719 |
T3720 |
nummod |
5,CTCTGAGCGTGGGGCCGTGCGCACAGCCGACTGC |
R2445 |
T3720 |
T3712 |
appos |
CTCTGAGCGTGGGGCCGTGCGCACAGCCGACTGC,GAGGCAGTCGGCTGTGCGCACGGCCCCACGCTC |
R2446 |
T3721 |
T3719 |
punct |
′,5 |
R2447 |
T3722 |
T3720 |
punct |
-,CTCTGAGCGTGGGGCCGTGCGCACAGCCGACTGC |
R2448 |
T3723 |
T3720 |
punct |
-,CTCTGAGCGTGGGGCCGTGCGCACAGCCGACTGC |
R2449 |
T3724 |
T3720 |
nummod |
3,CTCTGAGCGTGGGGCCGTGCGCACAGCCGACTGC |
R2450 |
T3725 |
T3720 |
punct |
′,CTCTGAGCGTGGGGCCGTGCGCACAGCCGACTGC |
R2451 |
T3726 |
T3497 |
punct |
", ",MRE1 |
R2452 |
T3727 |
T3728 |
compound |
Sepw1,MRE2 |
R2453 |
T3728 |
T3497 |
appos |
MRE2,MRE1 |
R2454 |
T3729 |
T3728 |
punct |
: ,MRE2 |
R2455 |
T3730 |
T3731 |
nummod |
5,ATGGTTTTGGGGGTGCGCAGGGGGTCTG |
R2456 |
T3731 |
T3728 |
appos |
ATGGTTTTGGGGGTGCGCAGGGGGTCTG,MRE2 |
R2457 |
T3732 |
T3730 |
punct |
′,5 |
R2458 |
T3733 |
T3731 |
punct |
-,ATGGTTTTGGGGGTGCGCAGGGGGTCTG |
R2459 |
T3734 |
T3731 |
punct |
-,ATGGTTTTGGGGGTGCGCAGGGGGTCTG |
R2460 |
T3735 |
T3731 |
nummod |
3,ATGGTTTTGGGGGTGCGCAGGGGGTCTG |
R2461 |
T3736 |
T3731 |
punct |
′,ATGGTTTTGGGGGTGCGCAGGGGGTCTG |
R2462 |
T3737 |
T3731 |
punct |
;,ATGGTTTTGGGGGTGCGCAGGGGGTCTG |
R2463 |
T3738 |
T3739 |
nummod |
5,CGACAGACCCCCTGCGCACCCCCAAAAC |
R2464 |
T3739 |
T3731 |
appos |
CGACAGACCCCCTGCGCACCCCCAAAAC,ATGGTTTTGGGGGTGCGCAGGGGGTCTG |
R2465 |
T3740 |
T3738 |
punct |
′,5 |
R2466 |
T3741 |
T3739 |
punct |
-,CGACAGACCCCCTGCGCACCCCCAAAAC |
R2467 |
T3742 |
T3739 |
punct |
-,CGACAGACCCCCTGCGCACCCCCAAAAC |
R2468 |
T3743 |
T3739 |
nummod |
3,CGACAGACCCCCTGCGCACCCCCAAAAC |
R2469 |
T3744 |
T3739 |
punct |
′,CGACAGACCCCCTGCGCACCCCCAAAAC |
R2470 |
T3745 |
T3497 |
punct |
", ",MRE1 |
R2471 |
T3746 |
T3747 |
compound |
Slc39a10,MRE1 |
R2472 |
T3747 |
T3497 |
appos |
MRE1,MRE1 |
R2473 |
T3748 |
T3747 |
punct |
: ,MRE1 |
R2474 |
T3749 |
T3750 |
nummod |
5,GAATACACGACTGGGTGCAGCCGGGGTTTGG |
R2475 |
T3750 |
T3747 |
appos |
GAATACACGACTGGGTGCAGCCGGGGTTTGG,MRE1 |
R2476 |
T3751 |
T3749 |
punct |
′,5 |
R2477 |
T3752 |
T3750 |
punct |
-,GAATACACGACTGGGTGCAGCCGGGGTTTGG |
R2478 |
T3753 |
T3750 |
punct |
-,GAATACACGACTGGGTGCAGCCGGGGTTTGG |
R2479 |
T3754 |
T3750 |
nummod |
3,GAATACACGACTGGGTGCAGCCGGGGTTTGG |
R2480 |
T3755 |
T3750 |
punct |
′,GAATACACGACTGGGTGCAGCCGGGGTTTGG |
R2481 |
T3756 |
T3750 |
punct |
;,GAATACACGACTGGGTGCAGCCGGGGTTTGG |
R2482 |
T3757 |
T3758 |
nummod |
5,GGTACCAAACCCCGGCTGCACCCAGTCGTGTA |
R2483 |
T3758 |
T3750 |
appos |
GGTACCAAACCCCGGCTGCACCCAGTCGTGTA,GAATACACGACTGGGTGCAGCCGGGGTTTGG |
R2484 |
T3759 |
T3757 |
punct |
′,5 |
R2485 |
T3760 |
T3758 |
punct |
-,GGTACCAAACCCCGGCTGCACCCAGTCGTGTA |
R2486 |
T3761 |
T3758 |
punct |
-,GGTACCAAACCCCGGCTGCACCCAGTCGTGTA |
R2487 |
T3762 |
T3758 |
nummod |
3,GGTACCAAACCCCGGCTGCACCCAGTCGTGTA |
R2488 |
T3763 |
T3758 |
punct |
′,GGTACCAAACCCCGGCTGCACCCAGTCGTGTA |
R2489 |
T3764 |
T3497 |
punct |
", ",MRE1 |
R2490 |
T3765 |
T3766 |
compound |
Slc39a10,MRE2 |
R2491 |
T3766 |
T3497 |
appos |
MRE2,MRE1 |
R2492 |
T3767 |
T3766 |
punct |
: ,MRE2 |
R2493 |
T3768 |
T3769 |
nummod |
5,GCGGAGAGGAGATGCACACGGCACTCG |
R2494 |
T3769 |
T3766 |
appos |
GCGGAGAGGAGATGCACACGGCACTCG,MRE2 |
R2495 |
T3770 |
T3768 |
punct |
′,5 |
R2496 |
T3771 |
T3769 |
punct |
-,GCGGAGAGGAGATGCACACGGCACTCG |
R2497 |
T3772 |
T3769 |
punct |
-,GCGGAGAGGAGATGCACACGGCACTCG |
R2498 |
T3773 |
T3769 |
nummod |
3,GCGGAGAGGAGATGCACACGGCACTCG |
R2499 |
T3774 |
T3769 |
punct |
′,GCGGAGAGGAGATGCACACGGCACTCG |
R2500 |
T3775 |
T3769 |
punct |
;,GCGGAGAGGAGATGCACACGGCACTCG |
R2501 |
T3776 |
T3777 |
nummod |
5,CACTCGAGTGCCGTGTGCATCTCCTCT |
R2502 |
T3777 |
T3769 |
appos |
CACTCGAGTGCCGTGTGCATCTCCTCT,GCGGAGAGGAGATGCACACGGCACTCG |
R2503 |
T3778 |
T3776 |
punct |
′,5 |
R2504 |
T3779 |
T3777 |
punct |
-,CACTCGAGTGCCGTGTGCATCTCCTCT |
R2505 |
T3780 |
T3777 |
punct |
-,CACTCGAGTGCCGTGTGCATCTCCTCT |
R2506 |
T3781 |
T3777 |
nummod |
3,CACTCGAGTGCCGTGTGCATCTCCTCT |
R2507 |
T3782 |
T3777 |
punct |
′,CACTCGAGTGCCGTGTGCATCTCCTCT |
R2508 |
T3783 |
T3497 |
punct |
", ",MRE1 |
R2509 |
T3784 |
T3785 |
nmod |
Specificity,protein |
R2510 |
T3785 |
T3786 |
nmod |
protein,sequence |
R2511 |
T3786 |
T3497 |
appos |
sequence,MRE1 |
R2512 |
T3787 |
T3785 |
nummod |
1,protein |
R2513 |
T3788 |
T3785 |
punct |
(,protein |
R2514 |
T3789 |
T3785 |
appos |
Sp1,protein |
R2515 |
T3790 |
T3786 |
punct |
),sequence |
R2516 |
T3791 |
T3786 |
compound |
binding,sequence |
R2517 |
T3792 |
T3793 |
punct |
(,10 |
R2518 |
T3793 |
T3786 |
parataxis |
10,sequence |
R2519 |
T3794 |
T3793 |
punct |
),10 |
R2520 |
T3795 |
T3497 |
punct |
: ,MRE1 |
R2521 |
T3796 |
T3797 |
nummod |
5,CGAGGCCCCGCCCAG |
R2522 |
T3797 |
T3497 |
appos |
CGAGGCCCCGCCCAG,MRE1 |
R2523 |
T3798 |
T3796 |
punct |
′,5 |
R2524 |
T3799 |
T3797 |
punct |
-,CGAGGCCCCGCCCAG |
R2525 |
T3800 |
T3797 |
punct |
-,CGAGGCCCCGCCCAG |
R2526 |
T3801 |
T3797 |
nummod |
3,CGAGGCCCCGCCCAG |
R2527 |
T3802 |
T3797 |
punct |
′,CGAGGCCCCGCCCAG |
R2528 |
T3803 |
T3797 |
punct |
;,CGAGGCCCCGCCCAG |
R2529 |
T3804 |
T3805 |
nummod |
5,TCGACTGGGCGGGGCCTCGAGct |
R2530 |
T3805 |
T3797 |
appos |
TCGACTGGGCGGGGCCTCGAGct,CGAGGCCCCGCCCAG |
R2531 |
T3806 |
T3804 |
punct |
′,5 |
R2532 |
T3807 |
T3805 |
punct |
-,TCGACTGGGCGGGGCCTCGAGct |
R2533 |
T3808 |
T3805 |
punct |
-,TCGACTGGGCGGGGCCTCGAGct |
R2534 |
T3809 |
T3805 |
nummod |
3,TCGACTGGGCGGGGCCTCGAGct |
R2535 |
T3810 |
T3805 |
punct |
′,TCGACTGGGCGGGGCCTCGAGct |
R2536 |
T3811 |
T3488 |
punct |
.,annealed |
R2537 |
T3873 |
T3888 |
compound |
Mtf1loxP,embryo |
R2538 |
T3874 |
T3898 |
conj |
streptomycin,serum |
R2539 |
T3875 |
T3876 |
compound |
Cell,culture |
R2540 |
T3878 |
T3879 |
amod |
Primary,fibroblasts |
R2541 |
T3879 |
T3881 |
nsubjpass |
fibroblasts,isolated |
R2542 |
T3880 |
T3879 |
amod |
embryonic,fibroblasts |
R2543 |
T3882 |
T3881 |
auxpass |
were,isolated |
R2544 |
T3883 |
T3881 |
prep |
from,isolated |
R2545 |
T3884 |
T3885 |
det |
a,day |
R2546 |
T3885 |
T3887 |
npadvmod |
day,old |
R2547 |
T3886 |
T3885 |
nummod |
12.5,day |
R2548 |
T3887 |
T3888 |
amod |
old,embryo |
R2549 |
T3888 |
T3883 |
pobj |
embryo,from |
R2550 |
T3889 |
T3888 |
compound |
mouse,embryo |
R2551 |
T3890 |
T3881 |
cc |
and,isolated |
R2552 |
T3891 |
T3881 |
conj |
grown,isolated |
R2553 |
T3892 |
T3891 |
prep |
in,grown |
R2554 |
T3893 |
T3892 |
pobj |
DMEM,in |
R2555 |
T3894 |
T3893 |
acl |
supplemented,DMEM |
R2556 |
T3895 |
T3894 |
prep |
with,supplemented |
R2557 |
T3896 |
T3897 |
nummod |
10,% |
R2558 |
T3897 |
T3898 |
nmod |
%,serum |
R2559 |
T3898 |
T3895 |
pobj |
serum,with |
R2560 |
T3899 |
T3898 |
amod |
fetal,serum |
R2561 |
T3900 |
T3898 |
amod |
bovine,serum |
R2562 |
T3901 |
T3902 |
punct |
(,ICN |
R2563 |
T3902 |
T3898 |
parataxis |
ICN,serum |
R2564 |
T3903 |
T3902 |
punct |
),ICN |
R2565 |
T3904 |
T3898 |
punct |
", ",serum |
R2566 |
T3905 |
T3906 |
nummod |
100,U |
R2567 |
T3906 |
T3874 |
nmod |
U,streptomycin |
R2568 |
T3907 |
T3908 |
punct |
/,ml |
R2569 |
T3908 |
T3906 |
prep |
ml,U |
R2570 |
T3909 |
T3874 |
compound |
penicillin,streptomycin |
R2571 |
T3910 |
T3874 |
punct |
–,streptomycin |
R2572 |
T3911 |
T3912 |
punct |
(,BRL |
R2573 |
T3912 |
T3874 |
parataxis |
BRL,streptomycin |
R2574 |
T3913 |
T3912 |
compound |
Gibco,BRL |
R2575 |
T3914 |
T3912 |
punct |
),BRL |
R2576 |
T3915 |
T3874 |
cc |
and,streptomycin |
R2577 |
T3916 |
T3917 |
nummod |
2,mM |
R2578 |
T3917 |
T3918 |
compound |
mM,glutamine |
R2579 |
T3918 |
T3874 |
conj |
glutamine,streptomycin |
R2580 |
T3919 |
T3918 |
compound |
l,glutamine |
R2581 |
T3920 |
T3918 |
punct |
-,glutamine |
R2582 |
T3921 |
T3922 |
punct |
(,BRL |
R2583 |
T3922 |
T3918 |
parataxis |
BRL,glutamine |
R2584 |
T3923 |
T3922 |
compound |
Gibco,BRL |
R2585 |
T3924 |
T3922 |
punct |
),BRL |
R2586 |
T3925 |
T3881 |
punct |
.,isolated |
R2587 |
T3927 |
T3928 |
nummod |
100,mm |
R2588 |
T3928 |
T3929 |
compound |
mm,plates |
R2589 |
T3929 |
T3930 |
nsubjpass |
plates,transfected |
R2590 |
T3931 |
T3929 |
prep |
with,plates |
R2591 |
T3932 |
T3933 |
amod |
primary,cells |
R2592 |
T3933 |
T3931 |
pobj |
cells,with |
R2593 |
T3934 |
T3930 |
auxpass |
were,transfected |
R2594 |
T3935 |
T3930 |
prep |
with,transfected |
R2595 |
T3936 |
T3937 |
nummod |
10,µg |
R2596 |
T3937 |
T3935 |
pobj |
µg,with |
R2597 |
T3938 |
T3937 |
prep |
of,µg |
R2598 |
T3939 |
T3940 |
det |
an,plasmid |
R2599 |
T3940 |
T3938 |
pobj |
plasmid,of |
R2600 |
T3941 |
T3940 |
compound |
expression,plasmid |
R2601 |
T3942 |
T3940 |
acl |
coding,plasmid |
R2602 |
T3943 |
T3942 |
prep |
for,coding |
R2603 |
T3944 |
T3945 |
amod |
simian,virus |
R2604 |
T3945 |
T3946 |
nmod |
virus,antigen |
R2605 |
T3946 |
T3943 |
pobj |
antigen,for |
R2606 |
T3947 |
T3945 |
nummod |
40,virus |
R2607 |
T3948 |
T3945 |
punct |
(,virus |
R2608 |
T3949 |
T3945 |
appos |
SV40,virus |
R2609 |
T3950 |
T3946 |
punct |
),antigen |
R2610 |
T3951 |
T3952 |
amod |
large,T |
R2611 |
T3952 |
T3946 |
compound |
T,antigen |
R2612 |
T3953 |
T3946 |
acl |
driven,antigen |
R2613 |
T3954 |
T3953 |
agent |
by,driven |
R2614 |
T3955 |
T3956 |
det |
the,promoter |
R2615 |
T3956 |
T3954 |
pobj |
promoter,by |
R2616 |
T3957 |
T3956 |
nmod |
cytomegalovirus,promoter |
R2617 |
T3958 |
T3957 |
punct |
(,cytomegalovirus |
R2618 |
T3959 |
T3957 |
appos |
CMV,cytomegalovirus |
R2619 |
T3960 |
T3956 |
punct |
),promoter |
R2620 |
T3961 |
T3930 |
punct |
", ",transfected |
R2621 |
T3962 |
T3930 |
advcl |
using,transfected |
R2622 |
T3963 |
T3964 |
nmod |
lipofectamine,reagent |
R2623 |
T3964 |
T3962 |
dobj |
reagent,using |
R2624 |
T3965 |
T3963 |
punct |
™,lipofectamine |
R2625 |
T3966 |
T3967 |
punct |
(,Invitrogen |
R2626 |
T3967 |
T3964 |
parataxis |
Invitrogen,reagent |
R2627 |
T3968 |
T3967 |
punct |
),Invitrogen |
R2628 |
T3969 |
T3962 |
prep |
according,using |
R2629 |
T3970 |
T3969 |
prep |
to,according |
R2630 |
T3971 |
T3972 |
det |
the,manufacturer |
R2631 |
T3972 |
T3973 |
poss |
manufacturer,instructions |
R2632 |
T3973 |
T3970 |
pobj |
instructions,to |
R2633 |
T3974 |
T3972 |
case |
's,manufacturer |
R2634 |
T3975 |
T3930 |
punct |
.,transfected |
R2635 |
T3977 |
T3978 |
compound |
Cell,foci |
R2636 |
T3978 |
T3979 |
nsubjpass |
foci,isolated |
R2637 |
T3980 |
T3979 |
auxpass |
were,isolated |
R2638 |
T3981 |
T3979 |
cc |
and,isolated |
R2639 |
T3982 |
T3983 |
det |
the,line |
R2640 |
T3983 |
T3989 |
nsubjpass |
line,derived |
R2641 |
T3984 |
T3983 |
amod |
immortalized,line |
R2642 |
T3985 |
T3986 |
nmod |
mouse,fibroblast |
R2643 |
T3986 |
T3983 |
compound |
fibroblast,line |
R2644 |
T3987 |
T3986 |
amod |
embryonic,fibroblast |
R2645 |
T3988 |
T3983 |
compound |
cell,line |
R2646 |
T3989 |
T3979 |
conj |
derived,isolated |
R2647 |
T3990 |
T3983 |
appos |
ckoC,line |
R2648 |
T3991 |
T3989 |
auxpass |
was,derived |
R2649 |
T3992 |
T3989 |
prep |
from,derived |
R2650 |
T3993 |
T3992 |
pobj |
one,from |
R2651 |
T3994 |
T3993 |
prep |
of,one |
R2652 |
T3995 |
T3994 |
pobj |
them,of |
R2653 |
T3996 |
T3989 |
punct |
.,derived |
R2654 |
T3998 |
T3999 |
det |
The,genotype |
R2655 |
T3999 |
T4001 |
nsubjpass |
genotype,confirmed |
R2656 |
T4000 |
T3999 |
compound |
Mtf1loxP,genotype |
R2657 |
T4002 |
T3999 |
prep |
of,genotype |
R2658 |
T4003 |
T4004 |
det |
this,line |
R2659 |
T4004 |
T4002 |
pobj |
line,of |
R2660 |
T4005 |
T4006 |
advmod |
as,as |
R2661 |
T4006 |
T3999 |
cc |
as,genotype |
R2662 |
T4007 |
T4006 |
advmod |
well,as |
R2663 |
T4008 |
T4009 |
det |
the,integration |
R2664 |
T4009 |
T3999 |
conj |
integration,genotype |
R2665 |
T4010 |
T4009 |
amod |
genomic,integration |
R2666 |
T4011 |
T4009 |
prep |
of,integration |
R2667 |
T4012 |
T4013 |
det |
the,antigen |
R2668 |
T4013 |
T4011 |
pobj |
antigen,of |
R2669 |
T4014 |
T4013 |
compound |
T,antigen |
R2670 |
T4015 |
T4001 |
auxpass |
were,confirmed |
R2671 |
T4016 |
T4001 |
agent |
by,confirmed |
R2672 |
T4017 |
T4016 |
pobj |
PCR,by |
R2673 |
T4018 |
T4001 |
punct |
.,confirmed |
R2674 |
T4020 |
T4021 |
nummod |
100,mm |
R2675 |
T4021 |
T4022 |
compound |
mm,plates |
R2676 |
T4022 |
T4023 |
nsubjpass |
plates,transfected |
R2677 |
T4024 |
T4022 |
prep |
with,plates |
R2678 |
T4025 |
T4026 |
det |
these,cells |
R2679 |
T4026 |
T4024 |
pobj |
cells,with |
R2680 |
T4027 |
T4023 |
auxpass |
were,transfected |
R2681 |
T4028 |
T4023 |
advmod |
further,transfected |
R2682 |
T4029 |
T4023 |
agent |
by,transfected |
R2683 |
T4030 |
T4031 |
det |
the,method |
R2684 |
T4031 |
T4029 |
pobj |
method,by |
R2685 |
T4032 |
T4033 |
compound |
calcium,phosphate |
R2686 |
T4033 |
T4031 |
compound |
phosphate,method |
R2687 |
T4034 |
T4035 |
punct |
(,30 |
R2688 |
T4035 |
T4031 |
parataxis |
30,method |
R2689 |
T4036 |
T4035 |
punct |
),30 |
R2690 |
T4037 |
T4031 |
prep |
with,method |
R2691 |
T4038 |
T4039 |
nummod |
19.6,µg |
R2692 |
T4039 |
T4037 |
pobj |
µg,with |
R2693 |
T4040 |
T4039 |
prep |
of,µg |
R2694 |
T4041 |
T4042 |
det |
an,plasmid |
R2695 |
T4042 |
T4040 |
pobj |
plasmid,of |
R2696 |
T4043 |
T4042 |
compound |
expression,plasmid |
R2697 |
T4044 |
T4042 |
prep |
for,plasmid |
R2698 |
T4045 |
T4046 |
compound |
Cre,recombinase |
R2699 |
T4046 |
T4044 |
pobj |
recombinase,for |
R2700 |
T4047 |
T4042 |
acl |
driven,plasmid |
R2701 |
T4048 |
T4047 |
agent |
by,driven |
R2702 |
T4049 |
T4050 |
det |
the,promoter |
R2703 |
T4050 |
T4048 |
pobj |
promoter,by |
R2704 |
T4051 |
T4050 |
compound |
CMV,promoter |
R2705 |
T4052 |
T4039 |
cc |
and,µg |
R2706 |
T4053 |
T4054 |
nummod |
0.4,µg |
R2707 |
T4054 |
T4039 |
conj |
µg,µg |
R2708 |
T4055 |
T4054 |
prep |
of,µg |
R2709 |
T4056 |
T4057 |
det |
an,plasmid |
R2710 |
T4057 |
T4055 |
pobj |
plasmid,of |
R2711 |
T4058 |
T4057 |
compound |
expression,plasmid |
R2712 |
T4059 |
T4057 |
prep |
for,plasmid |
R2713 |
T4060 |
T4061 |
det |
the,gene |
R2714 |
T4061 |
T4059 |
pobj |
gene,for |
R2715 |
T4062 |
T4061 |
compound |
neomycin,gene |
R2716 |
T4063 |
T4061 |
compound |
resistance,gene |
R2717 |
T4064 |
T4061 |
prep |
under,gene |
R2718 |
T4065 |
T4066 |
det |
the,control |
R2719 |
T4066 |
T4064 |
pobj |
control,under |
R2720 |
T4067 |
T4066 |
prep |
of,control |
R2721 |
T4068 |
T4069 |
det |
the,promoter |
R2722 |
T4069 |
T4067 |
pobj |
promoter,of |
R2723 |
T4070 |
T4069 |
compound |
TK,promoter |
R2724 |
T4071 |
T4023 |
punct |
.,transfected |
R2725 |
T4073 |
T4074 |
advmod |
Stably,transfected |
R2726 |
T4074 |
T4075 |
amod |
transfected,cells |
R2727 |
T4075 |
T4076 |
nsubjpass |
cells,selected |
R2728 |
T4077 |
T4076 |
auxpass |
were,selected |
R2729 |
T4078 |
T4076 |
prep |
in,selected |
R2730 |
T4079 |
T4080 |
det |
the,presence |
R2731 |
T4080 |
T4078 |
pobj |
presence,in |
R2732 |
T4081 |
T4080 |
prep |
of,presence |
R2733 |
T4082 |
T4083 |
nummod |
0.4,µg |
R2734 |
T4083 |
T4084 |
nmod |
µg,G418 |
R2735 |
T4084 |
T4081 |
pobj |
G418,of |
R2736 |
T4085 |
T4086 |
punct |
/,µl |
R2737 |
T4086 |
T4083 |
prep |
µl,µg |
R2738 |
T4087 |
T4088 |
punct |
(,Calbiochem |
R2739 |
T4088 |
T4084 |
parataxis |
Calbiochem,G418 |
R2740 |
T4089 |
T4088 |
punct |
),Calbiochem |
R2741 |
T4090 |
T4076 |
punct |
", ",selected |
R2742 |
T4091 |
T4092 |
amod |
isolated,clones |
R2743 |
T4092 |
T4093 |
nsubjpass |
clones,harvested |
R2744 |
T4093 |
T4076 |
conj |
harvested,selected |
R2745 |
T4094 |
T4092 |
prep |
of,clones |
R2746 |
T4095 |
T4096 |
amod |
resistant,cells |
R2747 |
T4096 |
T4094 |
pobj |
cells,of |
R2748 |
T4097 |
T4093 |
auxpass |
were,harvested |
R2749 |
T4098 |
T4093 |
cc |
and,harvested |
R2750 |
T4099 |
T4093 |
conj |
grown,harvested |
R2751 |
T4100 |
T4099 |
advmod |
independently,grown |
R2752 |
T4101 |
T4093 |
punct |
", ",harvested |
R2753 |
T4102 |
T4093 |
cc |
and,harvested |
R2754 |
T4103 |
T4104 |
det |
the,expression |
R2755 |
T4104 |
T4105 |
nsubjpass |
expression,analyzed |
R2756 |
T4105 |
T4093 |
conj |
analyzed,harvested |
R2757 |
T4106 |
T4104 |
prep |
of,expression |
R2758 |
T4107 |
T4108 |
compound |
Cre,recombinase |
R2759 |
T4108 |
T4106 |
pobj |
recombinase,of |
R2760 |
T4109 |
T4104 |
cc |
and,expression |
R2761 |
T4110 |
T4104 |
conj |
excision,expression |
R2762 |
T4111 |
T4110 |
prep |
of,excision |
R2763 |
T4112 |
T4113 |
nmod |
exons,3 |
R2764 |
T4113 |
T4111 |
pobj |
3,of |
R2765 |
T4114 |
T4113 |
cc |
and,3 |
R2766 |
T4115 |
T4113 |
conj |
4,3 |
R2767 |
T4116 |
T4113 |
prep |
of,3 |
R2768 |
T4117 |
T4116 |
pobj |
Mtf1,of |
R2769 |
T4118 |
T4105 |
auxpass |
were,analyzed |
R2770 |
T4119 |
T4105 |
agent |
by,analyzed |
R2771 |
T4120 |
T4121 |
compound |
RT,PCR |
R2772 |
T4121 |
T4119 |
pobj |
PCR,by |
R2773 |
T4122 |
T4121 |
punct |
–,PCR |
R2774 |
T4123 |
T4105 |
punct |
.,analyzed |
R2775 |
T4125 |
T4126 |
det |
The,lines |
R2776 |
T4126 |
T4128 |
nsubjpass |
lines,chosen |
R2777 |
T4127 |
T4126 |
compound |
cell,lines |
R2778 |
T4129 |
T4126 |
appos |
delC19,lines |
R2779 |
T4130 |
T4129 |
punct |
", ",delC19 |
R2780 |
T4131 |
T4129 |
conj |
delC21,delC19 |
R2781 |
T4132 |
T4131 |
cc |
and,delC21 |
R2782 |
T4133 |
T4131 |
conj |
delC23,delC21 |
R2783 |
T4134 |
T4126 |
prep |
with,lines |
R2784 |
T4135 |
T4136 |
det |
a,deletion |
R2785 |
T4136 |
T4134 |
pobj |
deletion,with |
R2786 |
T4137 |
T4136 |
prep |
of,deletion |
R2787 |
T4138 |
T4137 |
pobj |
Mtf1,of |
R2788 |
T4139 |
T4128 |
auxpass |
were,chosen |
R2789 |
T4140 |
T4128 |
prep |
for,chosen |
R2790 |
T4141 |
T4142 |
amod |
further,experiments |
R2791 |
T4142 |
T4140 |
pobj |
experiments,for |
R2792 |
T4143 |
T4128 |
punct |
.,chosen |
R2795 |
T4279 |
T4280 |
compound |
Cytotoxicity,assay |
R2796 |
T4282 |
T4283 |
nsubjpass |
Samples,plated |
R2797 |
T4284 |
T4282 |
prep |
of,Samples |
R2798 |
T4285 |
T4286 |
quantmod |
1,104 |
R2799 |
T4286 |
T4288 |
nummod |
104,cells |
R2800 |
T4287 |
T4286 |
punct |
×,104 |
R2801 |
T4288 |
T4284 |
pobj |
cells,of |
R2802 |
T4289 |
T4290 |
punct |
/,well |
R2803 |
T4290 |
T4288 |
prep |
well,cells |
R2804 |
T4291 |
T4283 |
auxpass |
were,plated |
R2805 |
T4292 |
T4283 |
prep |
in,plated |
R2806 |
T4293 |
T4294 |
nummod |
96,well |
R2807 |
T4294 |
T4296 |
compound |
well,plates |
R2808 |
T4295 |
T4294 |
punct |
-,well |
R2809 |
T4296 |
T4292 |
pobj |
plates,in |
R2810 |
T4297 |
T4298 |
compound |
tissue,culture |
R2811 |
T4298 |
T4296 |
compound |
culture,plates |
R2812 |
T4299 |
T4283 |
cc |
and,plated |
R2813 |
T4300 |
T4283 |
conj |
allowed,plated |
R2814 |
T4301 |
T4302 |
aux |
to,adhere |
R2815 |
T4302 |
T4300 |
xcomp |
adhere,allowed |
R2816 |
T4303 |
T4302 |
prep |
for,adhere |
R2817 |
T4304 |
T4305 |
nummod |
24,h |
R2818 |
T4305 |
T4303 |
pobj |
h,for |
R2819 |
T4306 |
T4283 |
punct |
.,plated |
R2820 |
T4308 |
T4309 |
det |
The,cells |
R2821 |
T4309 |
T4310 |
nsubjpass |
cells,pre-incubated |
R2822 |
T4311 |
T4310 |
auxpass |
were,pre-incubated |
R2823 |
T4312 |
T4310 |
advmod |
then,pre-incubated |
R2824 |
T4313 |
T4310 |
prep |
for,pre-incubated |
R2825 |
T4314 |
T4315 |
nummod |
24,h |
R2826 |
T4315 |
T4313 |
pobj |
h,for |
R2827 |
T4316 |
T4310 |
prep |
in,pre-incubated |
R2828 |
T4317 |
T4316 |
pobj |
medium,in |
R2829 |
T4318 |
T4317 |
acl |
containing,medium |
R2830 |
T4319 |
T4320 |
nummod |
0,µM |
R2831 |
T4320 |
T4318 |
dobj |
µM,containing |
R2832 |
T4321 |
T4319 |
punct |
", ",0 |
R2833 |
T4322 |
T4319 |
conj |
5,0 |
R2834 |
T4323 |
T4322 |
punct |
", ",5 |
R2835 |
T4324 |
T4322 |
conj |
10,5 |
R2836 |
T4325 |
T4324 |
punct |
", ",10 |
R2837 |
T4326 |
T4324 |
conj |
25,10 |
R2838 |
T4327 |
T4326 |
cc |
or,25 |
R2839 |
T4328 |
T4326 |
conj |
50,25 |
R2840 |
T4329 |
T4330 |
nmod |
l,sulfoximine |
R2841 |
T4330 |
T4320 |
appos |
sulfoximine,µM |
R2842 |
T4331 |
T4330 |
punct |
-,sulfoximine |
R2843 |
T4332 |
T4330 |
nmod |
buthionine,sulfoximine |
R2844 |
T4333 |
T4330 |
punct |
-,sulfoximine |
R2845 |
T4334 |
T4330 |
punct |
[,sulfoximine |
R2846 |
T4335 |
T4330 |
nmod |
S,sulfoximine |
R2847 |
T4336 |
T4330 |
punct |
",",sulfoximine |
R2848 |
T4337 |
T4330 |
nmod |
R,sulfoximine |
R2849 |
T4338 |
T4330 |
punct |
],sulfoximine |
R2850 |
T4339 |
T4330 |
punct |
-,sulfoximine |
R2851 |
T4340 |
T4330 |
punct |
(,sulfoximine |
R2852 |
T4341 |
T4330 |
appos |
BSO,sulfoximine |
R2853 |
T4342 |
T4320 |
punct |
),µM |
R2854 |
T4343 |
T4344 |
punct |
(,Sigma |
R2855 |
T4344 |
T4320 |
parataxis |
Sigma,µM |
R2856 |
T4345 |
T4344 |
punct |
),Sigma |
R2857 |
T4346 |
T4320 |
punct |
", ",µM |
R2858 |
T4347 |
T4348 |
det |
a,drug |
R2859 |
T4348 |
T4320 |
appos |
drug,µM |
R2860 |
T4349 |
T4350 |
dep |
that,inhibits |
R2861 |
T4350 |
T4348 |
relcl |
inhibits,drug |
R2862 |
T4351 |
T4352 |
compound |
glutathione,synthesis |
R2863 |
T4352 |
T4350 |
dobj |
synthesis,inhibits |
R2864 |
T4353 |
T4354 |
punct |
(,31 |
R2865 |
T4354 |
T4350 |
parataxis |
31,inhibits |
R2866 |
T4355 |
T4354 |
punct |
),31 |
R2867 |
T4356 |
T4310 |
punct |
.,pre-incubated |
R2868 |
T4358 |
T4359 |
advmod |
Later,exposed |
R2869 |
T4360 |
T4359 |
punct |
", ",exposed |
R2870 |
T4361 |
T4359 |
nsubjpass |
cells,exposed |
R2871 |
T4362 |
T4359 |
auxpass |
were,exposed |
R2872 |
T4363 |
T4359 |
prep |
to,exposed |
R2873 |
T4364 |
T4365 |
nummod |
0,µM |
R2874 |
T4365 |
T4372 |
compound |
µM,CdCl2 |
R2875 |
T4366 |
T4364 |
punct |
", ",0 |
R2876 |
T4367 |
T4364 |
conj |
5,0 |
R2877 |
T4368 |
T4367 |
punct |
", ",5 |
R2878 |
T4369 |
T4367 |
conj |
10,5 |
R2879 |
T4370 |
T4369 |
cc |
or,10 |
R2880 |
T4371 |
T4369 |
conj |
20,10 |
R2881 |
T4372 |
T4363 |
pobj |
CdCl2,to |
R2882 |
T4373 |
T4359 |
prep |
in,exposed |
R2883 |
T4374 |
T4375 |
det |
the,medium |
R2884 |
T4375 |
T4373 |
pobj |
medium,in |
R2885 |
T4376 |
T4375 |
amod |
specified,medium |
R2886 |
T4377 |
T4375 |
compound |
pre-incubation,medium |
R2887 |
T4378 |
T4359 |
prep |
for,exposed |
R2888 |
T4379 |
T4380 |
det |
an,h |
R2889 |
T4380 |
T4378 |
pobj |
h,for |
R2890 |
T4381 |
T4380 |
amod |
additional,h |
R2891 |
T4382 |
T4380 |
nummod |
24,h |
R2892 |
T4383 |
T4359 |
punct |
.,exposed |
R2893 |
T4385 |
T4386 |
nsubjpass |
Cytotoxicity,determined |
R2894 |
T4387 |
T4386 |
auxpass |
was,determined |
R2895 |
T4388 |
T4386 |
agent |
by,determined |
R2896 |
T4389 |
T4390 |
det |
the,Kit |
R2897 |
T4390 |
T4388 |
pobj |
Kit,by |
R2898 |
T4391 |
T4392 |
npadvmod |
MTT,based |
R2899 |
T4392 |
T4390 |
amod |
based,Kit |
R2900 |
T4393 |
T4394 |
punct |
(,bromid |
R2901 |
T4394 |
T4391 |
parataxis |
bromid,MTT |
R2902 |
T4395 |
T4396 |
nummod |
3,dimethylthiazol |
R2903 |
T4396 |
T4394 |
nmod |
dimethylthiazol,bromid |
R2904 |
T4397 |
T4396 |
punct |
-,dimethylthiazol |
R2905 |
T4398 |
T4396 |
punct |
[,dimethylthiazol |
R2906 |
T4399 |
T4396 |
nummod |
4,dimethylthiazol |
R2907 |
T4400 |
T4396 |
punct |
",",dimethylthiazol |
R2908 |
T4401 |
T4396 |
nummod |
5,dimethylthiazol |
R2909 |
T4402 |
T4396 |
punct |
-,dimethylthiazol |
R2910 |
T4403 |
T4396 |
punct |
-,dimethylthiazol |
R2911 |
T4404 |
T4405 |
nummod |
2,yl |
R2912 |
T4405 |
T4396 |
appos |
yl,dimethylthiazol |
R2913 |
T4406 |
T4405 |
punct |
-,yl |
R2914 |
T4407 |
T4396 |
punct |
],dimethylthiazol |
R2915 |
T4408 |
T4396 |
punct |
-,dimethylthiazol |
R2916 |
T4409 |
T4396 |
nummod |
2,dimethylthiazol |
R2917 |
T4410 |
T4394 |
punct |
",",bromid |
R2918 |
T4411 |
T4412 |
nummod |
5,diphenyl |
R2919 |
T4412 |
T4394 |
compound |
diphenyl,bromid |
R2920 |
T4413 |
T4412 |
punct |
-,diphenyl |
R2921 |
T4414 |
T4394 |
compound |
tetrazolium,bromid |
R2922 |
T4415 |
T4394 |
punct |
),bromid |
R2923 |
T4416 |
T4392 |
punct |
-,based |
R2924 |
T4417 |
T4390 |
compound |
Cell,Kit |
R2925 |
T4418 |
T4390 |
compound |
Proliferation,Kit |
R2926 |
T4419 |
T4390 |
nummod |
I,Kit |
R2927 |
T4420 |
T4421 |
punct |
(,Roche |
R2928 |
T4421 |
T4390 |
parataxis |
Roche,Kit |
R2929 |
T4422 |
T4421 |
punct |
),Roche |
R2930 |
T4423 |
T4386 |
prep |
according,determined |
R2931 |
T4424 |
T4423 |
prep |
to,according |
R2932 |
T4425 |
T4426 |
det |
the,manufacturer |
R2933 |
T4426 |
T4427 |
poss |
manufacturer,instructions |
R2934 |
T4427 |
T4424 |
pobj |
instructions,to |
R2935 |
T4428 |
T4426 |
case |
's,manufacturer |
R2936 |
T4429 |
T4386 |
punct |
.,determined |
R1329 |
T2196 |
T2190 |
appos |
cre,transgene |
R1470 |
T2341 |
T2322 |
conj |
H2O,CdSO4 |
R1577 |
T2628 |
T2626 |
pobj |
Kit,with |
R1721 |
T2786 |
T2782 |
dobj |
genes,obtain |
R2161 |
T3430 |
T3425 |
dobj |
oligonucleotides,incubating |