PMC:1253828 / 5385-15039 JSON TXT

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Id	Subject	Object	Predicate	Lexical cue
T1758	23-33	NN	denotes	Generation
T1759	34-36	IN	denotes	of
T1760	37-41	NN	denotes	Mtf1
T1761	63-67	NNS	denotes	mice
T1762	42-53	JJ	denotes	conditional
T1763	54-62	NN	denotes	knockout
T1764	68-71	CC	denotes	and
T1765	72-77	NN	denotes	liver
T1766	78-86	JJ	denotes	specific
T1767	77-78	HYPH	denotes	-
T1768	87-95	NN	denotes	deletion
T1769	95-200	sentence	denotes	Mtf1 conditional knockout mice were generated in collaboration with Dr Michael Leviten (San Carlos, CA).
T1770	96-100	NN	denotes	Mtf1
T1771	122-126	NNS	denotes	mice
T1772	101-112	JJ	denotes	conditional
T1773	113-121	NN	denotes	knockout
T1774	132-141	VBN	denotes	generated
T1775	127-131	VBD	denotes	were
T1776	142-144	IN	denotes	in
T1777	145-158	NN	denotes	collaboration
T1778	159-163	IN	denotes	with
T1779	164-166	NNP	denotes	Dr
T1780	175-182	NNP	denotes	Leviten
T1781	167-174	NNP	denotes	Michael
T1782	183-184	-LRB-	denotes	(
T1783	188-194	NNP	denotes	Carlos
T1784	184-187	NNP	denotes	San
T1785	194-196	,	denotes	,
T1786	196-198	NNP	denotes	CA
T1787	198-199	-RRB-	denotes	)
T1788	199-200	.	denotes	.
T1789	200-349	sentence	denotes	Two genomic clones containing exons 3 to 6 of Mtf1 were used to construct a gene targeting vector for homologous recombination (Supplementary Data).
T1790	201-204	CD	denotes	Two
T1791	213-219	NNS	denotes	clones
T1792	205-212	JJ	denotes	genomic
T1793	257-261	VBN	denotes	used
T1794	220-230	VBG	denotes	containing
T1795	231-236	NNS	denotes	exons
T1796	237-238	CD	denotes	3
T1797	239-241	IN	denotes	to
T1798	242-243	CD	denotes	6
T1799	244-246	IN	denotes	of
T1800	247-251	NN	denotes	Mtf1
T1801	252-256	VBD	denotes	were
T1802	262-264	TO	denotes	to
T1803	265-274	VB	denotes	construct
T1804	275-276	DT	denotes	a
T1805	292-298	NN	denotes	vector
T1806	277-281	NN	denotes	gene
T1807	282-291	NN	denotes	targeting
T1808	299-302	IN	denotes	for
T1809	303-313	JJ	denotes	homologous
T1810	314-327	NN	denotes	recombination
T1811	328-329	-LRB-	denotes	(
T1812	343-347	NNS	denotes	Data
T1813	329-342	JJ	denotes	Supplementary
T1814	347-348	-RRB-	denotes	)
T1815	348-349	.	denotes	.
T1816	349-532	sentence	denotes	A neomycin resistance cassette (PGK-neo) flanked by two loxP sites was cloned into the SacI site 5′ of exon 3 of Mtf1, the third loxP site was cloned into the ScaI site 3′ of exon 4.
T1817	350-351	DT	denotes	A
T1818	372-380	NN	denotes	cassette
T1819	352-360	NN	denotes	neomycin
T1820	361-371	NN	denotes	resistance
T1821	421-427	VBN	denotes	cloned
T1822	381-382	-LRB-	denotes	(
T1823	382-385	NN	denotes	PGK
T1824	386-389	NN	denotes	neo
T1825	385-386	HYPH	denotes	-
T1826	389-390	-RRB-	denotes	)
T1827	391-398	VBN	denotes	flanked
T1828	399-401	IN	denotes	by
T1829	402-405	CD	denotes	two
T1830	411-416	NNS	denotes	sites
T1831	406-410	NN	denotes	loxP
T1832	417-420	VBD	denotes	was
T1833	493-499	VBN	denotes	cloned
T1834	428-432	IN	denotes	into
T1835	433-436	DT	denotes	the
T1836	442-446	NN	denotes	site
T1837	437-441	NN	denotes	SacI
T1838	447-448	CD	denotes	5
T1839	448-449	SYM	denotes	′
T1840	450-452	IN	denotes	of
T1841	453-457	NN	denotes	exon
T1842	458-459	CD	denotes	3
T1843	460-462	IN	denotes	of
T1844	463-467	NN	denotes	Mtf1
T1845	467-469	,	denotes	,
T1846	469-472	DT	denotes	the
T1847	484-488	NN	denotes	site
T1848	473-478	JJ	denotes	third
T1849	479-483	NN	denotes	loxP
T1850	489-492	VBD	denotes	was
T1851	500-504	IN	denotes	into
T1852	505-508	DT	denotes	the
T1853	514-518	NN	denotes	site
T1854	509-513	NN	denotes	ScaI
T1855	519-520	CD	denotes	3
T1856	520-521	SYM	denotes	′
T1857	522-524	IN	denotes	of
T1858	525-529	NN	denotes	exon
T1859	530-531	CD	denotes	4
T1860	531-532	.	denotes	.
T1861	532-609	sentence	denotes	A thymidine kinase (TK) cassette was inserted in the HpaI site 3′ of exon 6.
T1862	533-534	DT	denotes	A
T1863	557-565	NN	denotes	cassette
T1864	535-544	NN	denotes	thymidine
T1865	545-551	NN	denotes	kinase
T1866	552-553	-LRB-	denotes	(
T1867	553-555	NN	denotes	TK
T1868	555-556	-RRB-	denotes	)
T1869	570-578	VBN	denotes	inserted
T1870	566-569	VBD	denotes	was
T1871	579-581	IN	denotes	in
T1872	582-585	DT	denotes	the
T1873	591-595	NN	denotes	site
T1874	586-590	NN	denotes	HpaI
T1875	596-597	CD	denotes	3
T1876	597-598	SYM	denotes	′
T1877	599-601	IN	denotes	of
T1878	602-606	NN	denotes	exon
T1879	607-608	CD	denotes	6
T1880	608-609	.	denotes	.
T1881	609-802	sentence	denotes	129 ES cells were electroporated with the linearized targeting vector, selected in the presence of G418 and FIAU, and screened for correct integration events by PCR and Southern blot analysis.
T1882	610-613	CD	denotes	129
T1883	617-622	NNS	denotes	cells
T1884	614-616	NN	denotes	ES
T1885	628-642	VBN	denotes	electroporated
T1886	623-627	VBD	denotes	were
T1887	643-647	IN	denotes	with
T1888	648-651	DT	denotes	the
T1889	673-679	NN	denotes	vector
T1890	652-662	VBN	denotes	linearized
T1891	663-672	NN	denotes	targeting
T1892	679-681	,	denotes	,
T1893	681-689	VBN	denotes	selected
T1894	690-692	IN	denotes	in
T1895	693-696	DT	denotes	the
T1896	697-705	NN	denotes	presence
T1897	706-708	IN	denotes	of
T1898	709-713	NN	denotes	G418
T1899	714-717	CC	denotes	and
T1900	718-722	NN	denotes	FIAU
T1901	722-724	,	denotes	,
T1902	724-727	CC	denotes	and
T1903	728-736	VBN	denotes	screened
T1904	737-740	IN	denotes	for
T1905	741-748	JJ	denotes	correct
T1906	761-767	NNS	denotes	events
T1907	749-760	NN	denotes	integration
T1908	768-770	IN	denotes	by
T1909	771-774	NN	denotes	PCR
T1910	793-801	NN	denotes	analysis
T1911	775-778	CC	denotes	and
T1912	779-787	NNP	denotes	Southern
T1913	788-792	NN	denotes	blot
T1914	801-802	.	denotes	.
T1915	802-1025	sentence	denotes	Transient expression of Cre recombinase led to removal of the PGK-neo cassette, and mice carrying the modified Mtf1loxP allele were generated by injection of positive clones into C57Bl/6 blastocysts and subsequent crosses.
T1916	803-812	JJ	denotes	Transient
T1917	813-823	NN	denotes	expression
T1918	843-846	VBD	denotes	led
T1919	824-826	IN	denotes	of
T1920	827-830	NN	denotes	Cre
T1921	831-842	NN	denotes	recombinase
T1922	847-849	IN	denotes	to
T1923	850-857	NN	denotes	removal
T1924	858-860	IN	denotes	of
T1925	861-864	DT	denotes	the
T1926	873-881	NN	denotes	cassette
T1927	865-868	NN	denotes	PGK
T1928	869-872	NN	denotes	neo
T1929	868-869	HYPH	denotes	-
T1930	881-883	,	denotes	,
T1931	883-886	CC	denotes	and
T1932	887-891	NNS	denotes	mice
T1933	935-944	VBN	denotes	generated
T1934	892-900	VBG	denotes	carrying
T1935	901-904	DT	denotes	the
T1936	923-929	NN	denotes	allele
T1937	905-913	VBN	denotes	modified
T1938	914-922	NN	denotes	Mtf1loxP
T1939	930-934	VBD	denotes	were
T1940	945-947	IN	denotes	by
T1941	948-957	NN	denotes	injection
T1942	958-960	IN	denotes	of
T1943	961-969	JJ	denotes	positive
T1944	970-976	NNS	denotes	clones
T1945	977-981	IN	denotes	into
T1946	982-987	NN	denotes	C57Bl
T1947	990-1001	NNS	denotes	blastocysts
T1948	987-988	HYPH	denotes	/
T1949	988-989	CD	denotes	6
T1950	1002-1005	CC	denotes	and
T1951	1006-1016	JJ	denotes	subsequent
T1952	1017-1024	NNS	denotes	crosses
T1953	1024-1025	.	denotes	.
T1955	1026-1036	JJ	denotes	Homozygous
T1956	1058-1065	NNS	denotes	animals
T1957	1037-1048	JJ	denotes	conditional
T1958	1049-1057	NN	denotes	knockout
T1959	1087-1094	VBN	denotes	crossed
T1960	1066-1067	-LRB-	denotes	(
T1961	1067-1075	NN	denotes	Mtf1loxP
T1962	1076-1080	NN	denotes	loxP
T1963	1075-1076	HYPH	denotes	/
T1964	1080-1081	-RRB-	denotes	)
T1965	1082-1086	VBD	denotes	were
T1966	1095-1099	IN	denotes	with
T1967	1100-1103	DT	denotes	the
T1968	1131-1135	NN	denotes	line
T2099	1233-1234	.	denotes	.
T2100	1234-1488	sentence	denotes	The mice were genotyped by PCR using the following primers (Microsynth): Cre recombinase: 5′-CTATCCAGCAACATTTGGGCCAGC-3′; 5′-CCAGGTTACGGATATAGTTCATGAC-3′, Mtf1loxP or wild-type allele: 5′-CACACCCAGTTTGTGTATGTCTTC-3′; 5′-CAGTCACAAGCAAATTACCAAACACTGCC-3′.
T2101	1235-1238	DT	denotes	The
T2102	1239-1243	NNS	denotes	mice
T2103	1249-1258	VBN	denotes	genotyped
T2104	1244-1248	VBD	denotes	were
T2105	1259-1261	IN	denotes	by
T2106	1262-1265	NN	denotes	PCR
T2107	1266-1271	VBG	denotes	using
T2108	1272-1275	DT	denotes	the
T2109	1286-1293	NNS	denotes	primers
T2110	1276-1285	VBG	denotes	following
T2111	1294-1295	-LRB-	denotes	(
T2112	1295-1305	NNP	denotes	Microsynth
T2113	1305-1306	-RRB-	denotes	)
T2114	1306-1308	:	denotes	:
T2115	1308-1311	NN	denotes	Cre
T2116	1312-1323	NN	denotes	recombinase
T2117	1360-1385	NN	denotes	CCAGGTTACGGATATAGTTCATGAC
T2118	1323-1325	:	denotes	:
T2119	1325-1326	CD	denotes	5
T2120	1328-1352	NN	denotes	CTATCCAGCAACATTTGGGCCAGC
T2121	1326-1327	SYM	denotes	′
T2122	1327-1328	HYPH	denotes	-
T2123	1352-1353	HYPH	denotes	-
T2124	1353-1354	CD	denotes	3
T2125	1354-1355	SYM	denotes	′
T2126	1355-1356	:	denotes	;
T2127	1357-1358	CD	denotes	5
T2128	1358-1359	SYM	denotes	′
T2129	1359-1360	HYPH	denotes	-
T2130	1455-1484	NN	denotes	CAGTCACAAGCAAATTACCAAACACTGCC
T2131	1385-1386	HYPH	denotes	-
T2132	1386-1387	CD	denotes	3
T2133	1387-1388	SYM	denotes	′
T2134	1388-1390	,	denotes	,
T2135	1390-1398	NN	denotes	Mtf1loxP
T2136	1399-1401	CC	denotes	or
T2137	1402-1406	JJ	denotes	wild
T2138	1407-1411	NN	denotes	type
T2139	1406-1407	HYPH	denotes	-
T2140	1412-1418	NN	denotes	allele
T2141	1418-1420	:	denotes	:
T2142	1420-1421	CD	denotes	5
T2143	1423-1447	NN	denotes	CACACCCAGTTTGTGTATGTCTTC
T2144	1421-1422	SYM	denotes	′
T2145	1422-1423	HYPH	denotes	-
T2146	1447-1448	HYPH	denotes	-
T2147	1448-1449	CD	denotes	3
T2148	1449-1450	SYM	denotes	′
T2149	1450-1451	:	denotes	;
T2150	1452-1453	CD	denotes	5
T2151	1453-1454	SYM	denotes	′
T2152	1454-1455	HYPH	denotes	-
T2153	1484-1485	HYPH	denotes	-
T2154	1485-1486	CD	denotes	3
T2155	1486-1487	SYM	denotes	′
T2156	1487-1488	.	denotes	.
T2531	2321-2331	NN	denotes	Microarray
T2532	2332-2340	NN	denotes	analysis
T2533	2341-2344	CC	denotes	and
T2534	2345-2349	NNS	denotes	data
T2535	2350-2360	NN	denotes	processing
T2536	2360-2586	sentence	denotes	Total RNA was isolated from liver tissue of pI–pC-induced, mock- or cadmium-treated Mtf1Mx-cre and Mtf1loxP mice (n = 3 per genotype and respective treatment; all male) essentially as described by Chomczynski and Sacchi (27).
T2537	2361-2366	JJ	denotes	Total
T2538	2367-2370	NN	denotes	RNA
T2539	2375-2383	VBN	denotes	isolated
T2540	2371-2374	VBD	denotes	was
T2541	2384-2388	IN	denotes	from
T2542	2389-2394	NN	denotes	liver
T2543	2395-2401	NN	denotes	tissue
T2544	2402-2404	IN	denotes	of
T2545	2405-2407	NN	denotes	pI
T2546	2408-2410	NN	denotes	pC
T2547	2407-2408	HYPH	denotes	–
T2548	2411-2418	VBN	denotes	induced
T2549	2410-2411	HYPH	denotes	-
T2550	2469-2473	NNS	denotes	mice
T2551	2418-2420	,	denotes	,
T2552	2420-2424	JJ	denotes	mock
T2553	2437-2444	VBN	denotes	treated
T2554	2424-2425	HYPH	denotes	-
T2555	2426-2428	CC	denotes	or
T2556	2429-2436	NN	denotes	cadmium
T2557	2436-2437	HYPH	denotes	-
T2558	2445-2451	NN	denotes	Mtf1Mx
T2559	2452-2455	NN	denotes	cre
T2560	2451-2452	HYPH	denotes	-
T2561	2456-2459	CC	denotes	and
T2562	2460-2468	NN	denotes	Mtf1loxP
T2563	2474-2475	-LRB-	denotes	(
T2564	2524-2528	JJ	denotes	male
T2565	2475-2476	NN	denotes	n
T2566	2479-2480	CD	denotes	3
T2567	2477-2478	SYM	denotes	=
T2568	2481-2484	IN	denotes	per
T2569	2485-2493	NN	denotes	genotype
T2570	2494-2497	CC	denotes	and
T2571	2498-2508	JJ	denotes	respective
T2572	2509-2518	NN	denotes	treatment
T2573	2518-2519	:	denotes	;
T2574	2520-2523	DT	denotes	all
T2575	2528-2529	-RRB-	denotes	)
T2576	2530-2541	RB	denotes	essentially
T2577	2545-2554	VBN	denotes	described
T2578	2542-2544	IN	denotes	as
T2579	2555-2557	IN	denotes	by
T2580	2558-2569	NNP	denotes	Chomczynski
T2581	2570-2573	CC	denotes	and
T2582	2574-2580	NNP	denotes	Sacchi
T2583	2581-2582	-LRB-	denotes	(
T2584	2582-2584	CD	denotes	27
T2585	2584-2585	-RRB-	denotes	)
T2586	2585-2586	.	denotes	.
T2587	2586-2811	sentence	denotes	Gene expression analysis was performed in the Functional Genomics Center Zurich using GeneChip® Mouse Genome 430 2.0 Arrays (Affymetrix) according to the manufacturer's instructions and the following reagents and conditions.
T2588	2587-2591	NN	denotes	Gene
T2589	2592-2602	NN	denotes	expression
T2590	2603-2611	NN	denotes	analysis
T2591	2616-2625	VBN	denotes	performed
T2592	2612-2615	VBD	denotes	was
T2593	2626-2628	IN	denotes	in
T2594	2629-2632	DT	denotes	the
T2595	2653-2659	NNP	denotes	Center
T2596	2633-2643	NNP	denotes	Functional
T2597	2644-2652	NNP	denotes	Genomics
T2598	2660-2666	NNP	denotes	Zurich
T2599	2667-2672	VBG	denotes	using
T2600	2673-2682	NNP	denotes	GeneChip®
T2601	2704-2710	NNS	denotes	Arrays
T2602	2683-2688	NNP	denotes	Mouse
T2603	2689-2695	NNP	denotes	Genome
T2604	2696-2699	CD	denotes	430
T2605	2700-2703	CD	denotes	2.0
T2606	2711-2712	-LRB-	denotes	(
T2607	2712-2722	NNP	denotes	Affymetrix
T2608	2722-2723	-RRB-	denotes	)
T2609	2724-2733	VBG	denotes	according
T2610	2734-2736	IN	denotes	to
T2611	2737-2740	DT	denotes	the
T2612	2741-2753	NN	denotes	manufacturer
T2613	2756-2768	NNS	denotes	instructions
T2614	2753-2755	POS	denotes	's
T2615	2769-2772	CC	denotes	and
T2616	2773-2776	DT	denotes	the
T2617	2787-2795	NNS	denotes	reagents
T2618	2777-2786	VBG	denotes	following
T2619	2796-2799	CC	denotes	and
T2620	2800-2810	NNS	denotes	conditions
T2621	2810-2811	.	denotes	.
T2622	2811-2922	sentence	denotes	cDNA was synthesized with SuperScript™ Double-Stranded cDNA Synthesis Kit (Invitrogen), using 15 µg total RNA.
T2623	2812-2816	NN	denotes	cDNA
T2624	2821-2832	VBN	denotes	synthesized
T2625	2817-2820	VBD	denotes	was
T2626	2833-2837	IN	denotes	with
T2627	2838-2849	NNP	denotes	SuperScript
T2628	2882-2885	NN	denotes	Kit
T2629	2849-2850	SYM	denotes	™
T2630	2851-2857	JJ	denotes	Double
T2631	2858-2866	VBN	denotes	Stranded
T2632	2857-2858	HYPH	denotes	-
T2633	2867-2871	NN	denotes	cDNA
T2634	2872-2881	NN	denotes	Synthesis
T2635	2886-2887	-LRB-	denotes	(
T2636	2887-2897	NNP	denotes	Invitrogen
T2637	2897-2898	-RRB-	denotes	)
T2638	2898-2900	,	denotes	,
T2639	2900-2905	VBG	denotes	using
T2640	2906-2908	CD	denotes	15
T2641	2909-2911	NN	denotes	µg
T2642	2918-2921	NN	denotes	RNA
T2643	2912-2917	JJ	denotes	total
T2644	2921-2922	.	denotes	.
T2645	2922-3051	sentence	denotes	In vitro transcription was performed with BioArray™ High Yield™ RNA Transcript Labeling Kit (Enzo) and 3.5 to 6 µg of each cDNA.
T2646	2923-2925	FW	denotes	In
T2647	2926-2931	FW	denotes	vitro
T2648	2932-2945	NN	denotes	transcription
T2649	2950-2959	VBN	denotes	performed
T2650	2946-2949	VBD	denotes	was
T2651	2960-2964	IN	denotes	with
T2652	2965-2973	NNP	denotes	BioArray
T2653	3011-3014	NN	denotes	Kit
T2654	2973-2974	SYM	denotes	™
T2655	2975-2979	NNP	denotes	High
T2656	2980-2985	NNP	denotes	Yield
T2657	2985-2986	SYM	denotes	™
T2658	2987-2990	NN	denotes	RNA
T2659	2991-3001	NN	denotes	Transcript
T2660	3002-3010	NN	denotes	Labeling
T2661	3015-3016	-LRB-	denotes	(
T2662	3016-3020	NNP	denotes	Enzo
T2663	3020-3021	-RRB-	denotes	)
T2664	3022-3025	CC	denotes	and
T2665	3026-3029	CD	denotes	3.5
T2666	3033-3034	CD	denotes	6
T2667	3030-3032	IN	denotes	to
T2668	3035-3037	NN	denotes	µg
T2669	3038-3040	IN	denotes	of
T2670	3041-3045	DT	denotes	each
T2671	3046-3050	NN	denotes	cDNA
T2672	3050-3051	.	denotes	.
T2673	3051-3152	sentence	denotes	Clean-up of both cDNA and cRNA samples was done using GeneChip® Sample Clean-up Module (Affymetrix).
T2674	3052-3057	NN	denotes	Clean
T2675	3058-3060	NN	denotes	up
T2676	3057-3058	HYPH	denotes	-
T2677	3095-3099	VBN	denotes	done
T2678	3061-3063	IN	denotes	of
T2679	3064-3068	CC	denotes	both
T2680	3069-3073	NN	denotes	cDNA
T2681	3083-3090	NNS	denotes	samples
T2682	3074-3077	CC	denotes	and
T2683	3078-3082	NN	denotes	cRNA
T2684	3091-3094	VBD	denotes	was
T2685	3100-3105	VBG	denotes	using
T2686	3106-3115	NNP	denotes	GeneChip®
T2687	3132-3138	NNP	denotes	Module
T2688	3116-3122	NNP	denotes	Sample
T2689	3123-3128	NNP	denotes	Clean
T2690	3129-3131	NNP	denotes	up
T2691	3128-3129	HYPH	denotes	-
T2692	3139-3140	-LRB-	denotes	(
T2693	3140-3150	NNP	denotes	Affymetrix
T2694	3150-3151	-RRB-	denotes	)
T2695	3151-3152	.	denotes	.
T2696	3152-3270	sentence	denotes	For the automated washing and staining in the Affymetrix fluidics station 450, the protocol EukGE-Ws2v4_450 was used.
T2697	3153-3156	IN	denotes	For
T2698	3265-3269	VBN	denotes	used
T2699	3157-3160	DT	denotes	the
T2700	3171-3178	NN	denotes	washing
T2701	3161-3170	VBN	denotes	automated
T2702	3179-3182	CC	denotes	and
T2703	3183-3191	NN	denotes	staining
T2704	3192-3194	IN	denotes	in
T2705	3195-3198	DT	denotes	the
T2706	3219-3226	NN	denotes	station
T2707	3199-3209	NNP	denotes	Affymetrix
T2708	3210-3218	NN	denotes	fluidics
T2709	3227-3230	CD	denotes	450
T2710	3230-3232	,	denotes	,
T2711	3232-3235	DT	denotes	the
T2712	3251-3260	NN	denotes	Ws2v4_450
T2713	3236-3244	NN	denotes	protocol
T2714	3245-3250	NN	denotes	EukGE
T2715	3250-3251	HYPH	denotes	-
T2716	3261-3264	VBD	denotes	was
T2717	3269-3270	.	denotes	.
T2718	3270-3337	sentence	denotes	The probe arrays were scanned with the Affymetrix GS 3000 scanner.
T2719	3271-3274	DT	denotes	The
T2720	3281-3287	NNS	denotes	arrays
T2721	3275-3280	NN	denotes	probe
T2722	3293-3300	VBN	denotes	scanned
T2723	3288-3292	VBD	denotes	were
T2724	3301-3305	IN	denotes	with
T2725	3306-3309	DT	denotes	the
T2726	3329-3336	NN	denotes	scanner
T2727	3310-3320	NNP	denotes	Affymetrix
T2728	3321-3323	NNP	denotes	GS
T2729	3324-3328	CD	denotes	3000
T2730	3336-3337	.	denotes	.
T2731	3337-3409	sentence	denotes	Raw data are available at ArrayExpress (, accession number E-MEXP-438).
T2732	3338-3341	JJ	denotes	Raw
T2733	3342-3346	NNS	denotes	data
T2734	3347-3350	VBP	denotes	are
T2735	3351-3360	JJ	denotes	available
T2736	3361-3363	IN	denotes	at
T2737	3364-3376	NNP	denotes	ArrayExpress
T2738	3377-3378	-LRB-	denotes	(
T2739	3399-3403	NN	denotes	MEXP
T2740	3378-3380	,	denotes	,
T2741	3380-3389	NN	denotes	accession
T2742	3390-3396	NN	denotes	number
T2743	3397-3398	NN	denotes	E
T2744	3398-3399	HYPH	denotes	-
T2745	3403-3404	HYPH	denotes	-
T2746	3404-3407	CD	denotes	438
T2747	3407-3408	-RRB-	denotes	)
T2748	3408-3409	.	denotes	.
T2749	3409-3526	sentence	denotes	Data analysis was performed with GeneSpring 6.1 software (Silicon Genetics), applying a significance level P ≤ 0.05.
T2750	3410-3414	NNS	denotes	Data
T2751	3415-3423	NN	denotes	analysis
T2752	3428-3437	VBN	denotes	performed
T2753	3424-3427	VBD	denotes	was
T2754	3438-3442	IN	denotes	with
T2755	3443-3453	NNP	denotes	GeneSpring
T2756	3458-3466	NN	denotes	software
T2757	3454-3457	CD	denotes	6.1
T2758	3467-3468	-LRB-	denotes	(
T2759	3476-3484	NNP	denotes	Genetics
T2760	3468-3475	NNP	denotes	Silicon
T2761	3484-3485	-RRB-	denotes	)
T2762	3485-3487	,	denotes	,
T2763	3487-3495	VBG	denotes	applying
T2764	3496-3497	DT	denotes	a
T2765	3511-3516	NN	denotes	level
T2766	3498-3510	NN	denotes	significance
T2767	3517-3518	NN	denotes	P
T2768	3521-3525	CD	denotes	0.05
T2769	3519-3520	SYM	denotes	≤
T2770	3525-3526	.	denotes	.
T2771	3526-3643	sentence	denotes	Furthermore, multiple testing correction was used in addition to obtain cadmium-responsive, MTF-1-independent genes.
T2772	3527-3538	RB	denotes	Furthermore
T2773	3572-3576	VBN	denotes	used
T2774	3538-3540	,	denotes	,
T2775	3540-3548	JJ	denotes	multiple
T2776	3557-3567	NN	denotes	correction
T2777	3549-3556	NN	denotes	testing
T2778	3568-3571	VBD	denotes	was
T2779	3577-3579	IN	denotes	in
T2780	3580-3588	NN	denotes	addition
T2781	3589-3591	TO	denotes	to
T2782	3592-3598	VB	denotes	obtain
T2783	3599-3606	NN	denotes	cadmium
T2784	3607-3617	JJ	denotes	responsive
T2785	3606-3607	HYPH	denotes	-
T2786	3637-3642	NNS	denotes	genes
T2787	3617-3619	,	denotes	,
T2788	3619-3622	NN	denotes	MTF
T2789	3625-3636	JJ	denotes	independent
T2790	3622-3623	HYPH	denotes	-
T2791	3623-3624	CD	denotes	1
T2792	3624-3625	HYPH	denotes	-
T2793	3642-3643	.	denotes	.
T2794	3643-3832	sentence	denotes	Genes were considered to be differentially expressed if there was at least a 2-fold difference in expression levels of the compared experimental groups (genotype and respective treatment).
T2795	3644-3649	NNS	denotes	Genes
T2796	3655-3665	VBN	denotes	considered
T2797	3650-3654	VBD	denotes	were
T2798	3666-3668	TO	denotes	to
T2799	3687-3696	VBN	denotes	expressed
T2800	3669-3671	VB	denotes	be
T2801	3672-3686	RB	denotes	differentially
T2802	3697-3699	IN	denotes	if
T2803	3706-3709	VBD	denotes	was
T2804	3700-3705	EX	denotes	there
T2805	3710-3712	RB	denotes	at
T2806	3713-3718	RBS	denotes	least
T2807	3721-3727	JJ	denotes	2-fold
T2808	3719-3720	DT	denotes	a
T2809	3728-3738	NN	denotes	difference
T2810	3739-3741	IN	denotes	in
T2811	3742-3752	NN	denotes	expression
T2812	3753-3759	NNS	denotes	levels
T2813	3760-3762	IN	denotes	of
T2814	3763-3766	DT	denotes	the
T2815	3789-3795	NNS	denotes	groups
T2816	3767-3775	VBN	denotes	compared
T2817	3776-3788	JJ	denotes	experimental
T2818	3796-3797	-LRB-	denotes	(
T2819	3797-3805	NN	denotes	genotype
T2820	3806-3809	CC	denotes	and
T2821	3810-3820	JJ	denotes	respective
T2822	3821-3830	NN	denotes	treatment
T2823	3830-3831	-RRB-	denotes	)
T2824	3831-3832	.	denotes	.
T2825	3832-4017	sentence	denotes	The result was considered reliable if signal values for the respective gene were scored ‘present’ at least for all mice in one experimental group or for two mice in each of two groups.
T2826	3833-3836	DT	denotes	The
T2827	3837-3843	NN	denotes	result
T2828	3848-3858	VBN	denotes	considered
T2829	3844-3847	VBD	denotes	was
T2830	3859-3867	JJ	denotes	reliable
T2831	3868-3870	IN	denotes	if
T2832	3914-3920	VBN	denotes	scored
T2833	3871-3877	NN	denotes	signal
T2834	3878-3884	NNS	denotes	values
T2835	3885-3888	IN	denotes	for
T2836	3889-3892	DT	denotes	the
T2837	3904-3908	NN	denotes	gene
T2838	3893-3903	JJ	denotes	respective
T2839	3909-3913	VBD	denotes	were
T2840	3921-3922	``	denotes	‘
T2841	3922-3929	JJ	denotes	present
T2842	3929-3930	''	denotes	’
T2843	3931-3933	RB	denotes	at
T2844	3934-3939	RBS	denotes	least
T2845	3940-3943	IN	denotes	for
T2846	3944-3947	DT	denotes	all
T2847	3948-3952	NNS	denotes	mice
T2848	3953-3955	IN	denotes	in
T2849	3956-3959	CD	denotes	one
T2850	3973-3978	NN	denotes	group
T2851	3960-3972	JJ	denotes	experimental
T2852	3979-3981	CC	denotes	or
T2853	3982-3985	IN	denotes	for
T2854	3986-3989	CD	denotes	two
T2855	3990-3994	NNS	denotes	mice
T2856	3995-3997	IN	denotes	in
T2857	3998-4002	DT	denotes	each
T2858	4003-4005	IN	denotes	of
T2859	4006-4009	CD	denotes	two
T2860	4010-4016	NNS	denotes	groups
T2861	4016-4017	.	denotes	.
T2862	4017-4280	sentence	denotes	To screen for the presence of the MRE core consensus sequence TGCRCNC in the promoter region, the upstream sequences of the respective genes were obtained from the University of California Santa Cruz (UCSC) Genome Browser Database (; October/November 2004) (28).
T2863	4018-4020	TO	denotes	To
T2864	4021-4027	VB	denotes	screen
T2865	4164-4172	VBN	denotes	obtained
T2866	4028-4031	IN	denotes	for
T2867	4032-4035	DT	denotes	the
T2868	4036-4044	NN	denotes	presence
T2869	4045-4047	IN	denotes	of
T2870	4048-4051	DT	denotes	the
T2871	4071-4079	NN	denotes	sequence
T2872	4052-4055	NN	denotes	MRE
T2873	4056-4060	NN	denotes	core
T2874	4061-4070	NN	denotes	consensus
T2875	4080-4087	NN	denotes	TGCRCNC
T2876	4088-4090	IN	denotes	in
T2877	4091-4094	DT	denotes	the
T2878	4104-4110	NN	denotes	region
T2879	4095-4103	NN	denotes	promoter
T2880	4110-4112	,	denotes	,
T2881	4112-4115	DT	denotes	the
T2882	4125-4134	NNS	denotes	sequences
T2883	4116-4124	JJ	denotes	upstream
T2884	4135-4137	IN	denotes	of
T2885	4138-4141	DT	denotes	the
T2886	4153-4158	NNS	denotes	genes
T2887	4142-4152	JJ	denotes	respective
T2888	4159-4163	VBD	denotes	were
T2889	4173-4177	IN	denotes	from
T2890	4178-4181	DT	denotes	the
T2891	4240-4248	NNP	denotes	Database
T2892	4182-4192	NNP	denotes	University
T2893	4193-4195	IN	denotes	of
T2894	4196-4206	NNP	denotes	California
T2895	4213-4217	NNP	denotes	Cruz
T2896	4207-4212	NNP	denotes	Santa
T2897	4218-4219	-LRB-	denotes	(
T2898	4219-4223	NNP	denotes	UCSC
T2899	4223-4224	-RRB-	denotes	)
T2900	4225-4231	NNP	denotes	Genome
T2901	4232-4239	NNP	denotes	Browser
T2902	4249-4250	-LRB-	denotes	(
T2903	4260-4268	NNP	denotes	November
T2904	4250-4251	:	denotes	;
T2905	4252-4259	NNP	denotes	October
T2906	4259-4260	SYM	denotes	/
T2907	4269-4273	CD	denotes	2004
T2908	4273-4274	-RRB-	denotes	)
T2909	4275-4276	-LRB-	denotes	(
T2910	4276-4278	CD	denotes	28
T2911	4278-4279	-RRB-	denotes	)
T2912	4279-4280	.	denotes	.
T3025	4696-4697	SYM	denotes	′
T3026	4697-4698	HYPH	denotes	-
T3351	5514-5529	JJ	denotes	Electrophoretic
T3352	5539-5544	NN	denotes	shift
T3353	5530-5538	NN	denotes	mobility
T3354	5545-5550	NN	denotes	assay
T3355	5551-5552	-LRB-	denotes	(
T3356	5552-5556	NN	denotes	EMSA
T3357	5556-5557	-RRB-	denotes	)
T3358	5557-5761	sentence	denotes	Protein was extracted from liver tissue with T-PER™ Tissue Protein Extraction Reagent (Pierce) according to the manufacturer's instructions, using a 1:10 ratio of mouse tissue (mg) to T-PER reagent (µl).
T3359	5558-5565	NN	denotes	Protein
T3360	5570-5579	VBN	denotes	extracted
T3361	5566-5569	VBD	denotes	was
T3362	5580-5584	IN	denotes	from
T3363	5585-5590	NN	denotes	liver
T3364	5591-5597	NN	denotes	tissue
T3365	5598-5602	IN	denotes	with
T3366	5603-5604	NN	denotes	T
T3367	5605-5608	NN	denotes	PER
T3368	5604-5605	HYPH	denotes	-
T3369	5608-5609	SYM	denotes	™
T3370	5610-5616	NN	denotes	Tissue
T3371	5617-5624	NN	denotes	Protein
T3372	5625-5635	NN	denotes	Extraction
T3373	5636-5643	NN	denotes	Reagent
T3374	5644-5645	-LRB-	denotes	(
T3375	5645-5651	NNP	denotes	Pierce
T3376	5651-5652	-RRB-	denotes	)
T3377	5653-5662	VBG	denotes	according
T3378	5663-5665	IN	denotes	to
T3379	5666-5669	DT	denotes	the
T3380	5670-5682	NN	denotes	manufacturer
T3381	5685-5697	NNS	denotes	instructions
T3382	5682-5684	POS	denotes	's
T3383	5697-5699	,	denotes	,
T3384	5699-5704	VBG	denotes	using
T3385	5705-5706	DT	denotes	a
T3386	5712-5717	NN	denotes	ratio
T3387	5707-5708	CD	denotes	1
T3388	5708-5709	SYM	denotes	:
T3389	5709-5711	CD	denotes	10
T3390	5718-5720	IN	denotes	of
T3391	5721-5726	NN	denotes	mouse
T3392	5727-5733	NN	denotes	tissue
T3393	5734-5735	-LRB-	denotes	(
T3394	5735-5737	NN	denotes	mg
T3395	5737-5738	-RRB-	denotes	)
T3396	5739-5741	IN	denotes	to
T3397	5742-5743	NN	denotes	T
T3398	5744-5747	NN	denotes	PER
T3399	5743-5744	HYPH	denotes	-
T3400	5748-5755	NN	denotes	reagent
T3401	5756-5757	-LRB-	denotes	(
T3402	5757-5759	NN	denotes	µl
T3403	5759-5760	-RRB-	denotes	)
T3404	5760-5761	.	denotes	.
T3405	5761-5822	sentence	denotes	EMSA was essentially performed as previously described (10).
T3406	5762-5766	NN	denotes	EMSA
T3407	5783-5792	VBN	denotes	performed
T3408	5767-5770	VBD	denotes	was
T3409	5771-5782	RB	denotes	essentially
T3410	5793-5795	IN	denotes	as
T3411	5807-5816	VBN	denotes	described
T3412	5796-5806	RB	denotes	previously
T3413	5817-5818	-LRB-	denotes	(
T3414	5818-5820	CD	denotes	10
T3415	5820-5821	-RRB-	denotes	)
T3416	5821-5822	.	denotes	.
T3417	5822-5975	sentence	denotes	All binding reactions were carried out by incubating 2–5 fmol 32P-end-labeled double-stranded oligonucleotides with 100 to 130 µg liver protein extract.
T3418	5823-5826	DT	denotes	All
T3419	5835-5844	NNS	denotes	reactions
T3420	5827-5834	NN	denotes	binding
T3421	5850-5857	VBN	denotes	carried
T3422	5845-5849	VBD	denotes	were
T3423	5858-5861	RP	denotes	out
T3424	5862-5864	IN	denotes	by
T3425	5865-5875	VBG	denotes	incubating
T3426	5876-5877	CD	denotes	2
T3427	5878-5879	CD	denotes	5
T3428	5877-5878	SYM	denotes	–
T3429	5880-5884	NN	denotes	fmol
T3430	5917-5933	NNS	denotes	oligonucleotides
T3431	5885-5888	NN	denotes	32P
T3432	5893-5900	VBN	denotes	labeled
T3433	5888-5889	HYPH	denotes	-
T3434	5889-5892	NN	denotes	end
T3435	5892-5893	HYPH	denotes	-
T3436	5901-5907	JJ	denotes	double
T3437	5908-5916	VBN	denotes	stranded
T3438	5907-5908	HYPH	denotes	-
T3439	5934-5938	IN	denotes	with
T3440	5939-5942	CD	denotes	100
T3441	5946-5949	CD	denotes	130
T3442	5943-5945	IN	denotes	to
T3443	5950-5952	NN	denotes	µg
T3444	5967-5974	NN	denotes	extract
T3445	5953-5958	NN	denotes	liver
T3446	5959-5966	NN	denotes	protein
T3447	5974-5975	.	denotes	.
T3448	5975-6117	sentence	denotes	For competition experiments, 5 pmol of unlabeled competitor oligonucleotide was added to the binding reaction before addition of the extract.
T3449	5976-5979	IN	denotes	For
T3450	6056-6061	VBN	denotes	added
T3451	5980-5991	NN	denotes	competition
T3452	5992-6003	NNS	denotes	experiments
T3453	6003-6005	,	denotes	,
T3454	6005-6006	CD	denotes	5
T3455	6007-6011	NN	denotes	pmol
T3456	6012-6014	IN	denotes	of
T3457	6015-6024	JJ	denotes	unlabeled
T3458	6036-6051	NN	denotes	oligonucleotide
T3459	6025-6035	NN	denotes	competitor
T3460	6052-6055	VBD	denotes	was
T3461	6062-6064	IN	denotes	to
T3462	6065-6068	DT	denotes	the
T3463	6077-6085	NN	denotes	reaction
T3464	6069-6076	NN	denotes	binding
T3465	6086-6092	IN	denotes	before
T3466	6093-6101	NN	denotes	addition
T3467	6102-6104	IN	denotes	of
T3468	6105-6108	DT	denotes	the
T3469	6109-6116	NN	denotes	extract
T3470	6116-6117	.	denotes	.
T3471	6117-6189	sentence	denotes	All EMSA gels were developed using PhosphorImager (Molecular Dynamics).
T3472	6118-6121	DT	denotes	All
T3473	6127-6131	NNS	denotes	gels
T3474	6122-6126	NN	denotes	EMSA
T3475	6137-6146	VBN	denotes	developed
T3476	6132-6136	VBD	denotes	were
T3477	6147-6152	VBG	denotes	using
T3478	6153-6167	NNP	denotes	PhosphorImager
T3479	6168-6169	-LRB-	denotes	(
T3480	6179-6187	NNP	denotes	Dynamics
T3481	6169-6178	NNP	denotes	Molecular
T3482	6187-6188	-RRB-	denotes	)
T3483	6188-6189	.	denotes	.
T3484	6189-7651	sentence	denotes	The following oligonucleotides were annealed and used for the reactions: Csrp1 MRE1: 5′-GGAAACAAAACGGCGCGCACTCCGGCGC-3′; 5′-GGCTGCGC CGGAGTGCGCGCCGTTTTGT-3′, Csrp1 MRE2: 5′-TGTTGTGGTGCAGTGTGCAAAGCCTAC-3′; 5′-ACCAGTAGGCTTTGCACACTGCACCAC-3′, Csrp1 MRE3: 5′-GAGATCGCCATAGGGTGCAAAGAGAAG-3′; 5′-GTGACTTCTCTTTGCACCCTATGGCGA-3′, Csrp1 MRE4: 5′-TGTCTTATTCTGGAGTGCAAGTTAGTC-3′; 5′-AGGGGACTAACTTGCACTCCAGAATAA-3′, Gal4: 5′-TCCGGAGGACTGTCCTCCGG-3′; 5′-GCCGGAGGACAGTCCTCCGG-3′, MREd [MRE derived from mouse Mt1 promoter (10)]: 5′-CGAGGGAGCTCTGCACTCCGCCCGAAAAGTG-3′; 5′-TCGACACTTTTCGGGCGGAGTGCAGAGCTCCCTCGAGCT-3′, MRE-s [synthetic MRE consensus sequence (10)]: 5′-CGAGGGAGCTCTGCACACGGCCCGAAAAGTG-3′; 5′-TCGACACTTTTCGGGCCGTGTGCAGAGCTCCCTCGAGCT-3′, Ndrg1 MRE1: 5′-CAGCCCAGGCAGGGTGCAGCACGAG-3′; 5′-CCGCCTCGTGCTGCACCCTGCCTGG-3′, Ndrg1 MRE2: 5′-CACACGTTCGCTGCACACGCCGCGG-3′; 5′-GGGACCGCGGCGTGTGCAGCGAACG-3′, Ndrg1 MRE3,4: 5′-GGAGTCCTTATGCACACGCGCACGAGCGCGCACGGGCAC-3′; 5′-TGGTGTGCCCGTGCGCGCTCGTGCGCGTGTGCATAAGGAC-3′, Sepw1 MRE1: 5′-GAGGCAGTCGGCTGTGCGCACGGCCCCACGCTC-3′; 5′-CTCTGAGCGTGGGGCCGTGCGCACAGCCGACTGC-3′, Sepw1 MRE2: 5′-ATGGTTTTGGGGGTGCGCAGGGGGTCTG-3′; 5′-CGACAGACCCCCTGCGCACCCCCAAAAC-3′, Slc39a10 MRE1: 5′-GAATACACGACTGGGTGCAGCCGGGGTTTGG-3′; 5′-GGTACCAAACCCCGGCTGCACCCAGTCGTGTA-3′, Slc39a10 MRE2: 5′-GCGGAGAGGAGATGCACACGGCACTCG-3′; 5′-CACTCGAGTGCCGTGTGCATCTCCTCT-3′, Specificity protein 1 (Sp1) binding sequence (10): 5′-CGAGGCCCCGCCCAG-3′; 5′-TCGACTGGGCGGGGCCTCGAGct-3′.
T3485	6190-6193	DT	denotes	The
T3486	6204-6220	NNS	denotes	oligonucleotides
T3487	6194-6203	VBG	denotes	following
T3488	6226-6234	VBN	denotes	annealed
T3489	6221-6225	VBD	denotes	were
T3490	6235-6238	CC	denotes	and
T3491	6239-6243	VBN	denotes	used
T3492	6244-6247	IN	denotes	for
T3493	6248-6251	DT	denotes	the
T3494	6252-6261	NNS	denotes	reactions
T3495	6261-6263	:	denotes	:
T3496	6263-6268	NN	denotes	Csrp1
T3497	6269-6273	NN	denotes	MRE1
T3498	6273-6275	:	denotes	:
T3499	6275-6276	CD	denotes	5
T3500	6278-6306	NN	denotes	GGAAACAAAACGGCGCGCACTCCGGCGC
T3501	6276-6277	SYM	denotes	′
T3502	6277-6278	HYPH	denotes	-
T3503	6306-6307	HYPH	denotes	-
T3504	6307-6308	CD	denotes	3
T3505	6308-6309	SYM	denotes	′
T3506	6309-6310	:	denotes	;
T3507	6311-6312	CD	denotes	5
T3508	6323-6343	NN	denotes	CGGAGTGCGCGCCGTTTTGT
T3509	6312-6313	SYM	denotes	′
T3510	6313-6314	HYPH	denotes	-
T3511	6314-6322	NN	denotes	GGCTGCGC
T3512	6343-6344	HYPH	denotes	-
T3513	6344-6345	CD	denotes	3
T3514	6345-6346	SYM	denotes	′
T3515	6346-6348	,	denotes	,
T3516	6348-6353	NN	denotes	Csrp1
T3517	6354-6358	NN	denotes	MRE2
T3518	6358-6360	:	denotes	:
T3519	6360-6361	CD	denotes	5
T3520	6363-6390	NN	denotes	TGTTGTGGTGCAGTGTGCAAAGCCTAC
T3521	6361-6362	SYM	denotes	′
T3522	6362-6363	HYPH	denotes	-
T3523	6390-6391	HYPH	denotes	-
T3524	6391-6392	CD	denotes	3
T3525	6392-6393	SYM	denotes	′
T3526	6393-6394	:	denotes	;
T3527	6395-6396	CD	denotes	5
T3528	6398-6425	NN	denotes	ACCAGTAGGCTTTGCACACTGCACCAC
T3529	6396-6397	SYM	denotes	′
T3530	6397-6398	HYPH	denotes	-
T3531	6425-6426	HYPH	denotes	-
T3532	6426-6427	CD	denotes	3
T3533	6427-6428	SYM	denotes	′
T3534	6428-6430	,	denotes	,
T3535	6430-6435	NN	denotes	Csrp1
T3536	6436-6440	NN	denotes	MRE3
T3537	6440-6442	:	denotes	:
T3538	6442-6443	CD	denotes	5
T3539	6445-6472	NN	denotes	GAGATCGCCATAGGGTGCAAAGAGAAG
T3540	6443-6444	SYM	denotes	′
T3541	6444-6445	HYPH	denotes	-
T3542	6472-6473	HYPH	denotes	-
T3543	6473-6474	CD	denotes	3
T3544	6474-6475	SYM	denotes	′
T3545	6475-6476	:	denotes	;
T3546	6477-6478	CD	denotes	5
T3547	6480-6507	NN	denotes	GTGACTTCTCTTTGCACCCTATGGCGA
T3548	6478-6479	SYM	denotes	′
T3549	6479-6480	HYPH	denotes	-
T3550	6507-6508	HYPH	denotes	-
T3551	6508-6509	CD	denotes	3
T3552	6509-6510	SYM	denotes	′
T3553	6510-6512	,	denotes	,
T3554	6512-6517	NN	denotes	Csrp1
T3555	6518-6522	NN	denotes	MRE4
T3556	6522-6524	:	denotes	:
T3557	6524-6525	CD	denotes	5
T3558	6527-6554	NN	denotes	TGTCTTATTCTGGAGTGCAAGTTAGTC
T3559	6525-6526	SYM	denotes	′
T3560	6526-6527	HYPH	denotes	-
T3561	6554-6555	HYPH	denotes	-
T3562	6555-6556	CD	denotes	3
T3563	6556-6557	SYM	denotes	′
T3564	6557-6558	:	denotes	;
T3565	6559-6560	CD	denotes	5
T3566	6562-6589	NN	denotes	AGGGGACTAACTTGCACTCCAGAATAA
T3567	6560-6561	SYM	denotes	′
T3568	6561-6562	HYPH	denotes	-
T3569	6589-6590	HYPH	denotes	-
T3570	6590-6591	CD	denotes	3
T3571	6591-6592	SYM	denotes	′
T3572	6592-6594	,	denotes	,
T3573	6594-6598	NN	denotes	Gal4
T3574	6598-6600	:	denotes	:
T3575	6600-6601	CD	denotes	5
T3576	6603-6623	NN	denotes	TCCGGAGGACTGTCCTCCGG
T3577	6601-6602	SYM	denotes	′
T3578	6602-6603	HYPH	denotes	-
T3579	6623-6624	HYPH	denotes	-
T3580	6624-6625	CD	denotes	3
T3581	6625-6626	SYM	denotes	′
T3582	6626-6627	:	denotes	;
T3583	6628-6629	CD	denotes	5
T3584	6631-6651	NN	denotes	GCCGGAGGACAGTCCTCCGG
T3585	6629-6630	SYM	denotes	′
T3586	6630-6631	HYPH	denotes	-
T3587	6651-6652	HYPH	denotes	-
T3588	6652-6653	CD	denotes	3
T3589	6653-6654	SYM	denotes	′
T3590	6654-6656	,	denotes	,
T3591	6656-6660	NN	denotes	MREd
T3592	6661-6662	-LRB-	denotes	[
T3593	6662-6665	NN	denotes	MRE
T3594	6666-6673	VBN	denotes	derived
T3595	6674-6678	IN	denotes	from
T3596	6679-6684	NN	denotes	mouse
T3597	6689-6697	NN	denotes	promoter
T3598	6685-6688	NN	denotes	Mt1
T3599	6698-6699	-LRB-	denotes	(
T3600	6699-6701	CD	denotes	10
T3601	6701-6702	-RRB-	denotes	)
T3602	6702-6703	-RRB-	denotes	]
T3603	6703-6705	:	denotes	:
T3604	6705-6706	CD	denotes	5
T3605	6708-6739	NN	denotes	CGAGGGAGCTCTGCACTCCGCCCGAAAAGTG
T3606	6706-6707	SYM	denotes	′
T3607	6707-6708	HYPH	denotes	-
T3608	6739-6740	HYPH	denotes	-
T3609	6740-6741	CD	denotes	3
T3610	6741-6742	SYM	denotes	′
T3611	6742-6743	:	denotes	;
T3612	6744-6745	CD	denotes	5
T3613	6747-6786	NN	denotes	TCGACACTTTTCGGGCGGAGTGCAGAGCTCCCTCGAGCT
T3614	6745-6746	SYM	denotes	′
T3615	6746-6747	HYPH	denotes	-
T3616	6786-6787	HYPH	denotes	-
T3617	6787-6788	CD	denotes	3
T3618	6788-6789	SYM	denotes	′
T3619	6789-6791	,	denotes	,
T3620	6791-6794	NN	denotes	MRE
T3621	6795-6796	NN	denotes	s
T3622	6794-6795	HYPH	denotes	-
T3623	6797-6798	-LRB-	denotes	[
T3624	6798-6807	JJ	denotes	synthetic
T3625	6822-6830	NN	denotes	sequence
T3626	6808-6811	NN	denotes	MRE
T3627	6812-6821	NN	denotes	consensus
T3628	6831-6832	-LRB-	denotes	(
T3629	6832-6834	CD	denotes	10
T3630	6834-6835	-RRB-	denotes	)
T3631	6835-6836	-RRB-	denotes	]
T3632	6836-6838	:	denotes	:
T3633	6838-6839	CD	denotes	5
T3634	6841-6872	NN	denotes	CGAGGGAGCTCTGCACACGGCCCGAAAAGTG
T3635	6839-6840	SYM	denotes	′
T3636	6840-6841	HYPH	denotes	-
T3637	6872-6873	HYPH	denotes	-
T3638	6873-6874	CD	denotes	3
T3639	6874-6875	SYM	denotes	′
T3640	6875-6876	:	denotes	;
T3641	6877-6878	CD	denotes	5
T3642	6880-6919	NN	denotes	TCGACACTTTTCGGGCCGTGTGCAGAGCTCCCTCGAGCT
T3643	6878-6879	SYM	denotes	′
T3644	6879-6880	HYPH	denotes	-
T3645	6919-6920	HYPH	denotes	-
T3646	6920-6921	CD	denotes	3
T3647	6921-6922	SYM	denotes	′
T3648	6922-6924	,	denotes	,
T3649	6924-6929	NN	denotes	Ndrg1
T3650	6930-6934	NN	denotes	MRE1
T3651	6934-6936	:	denotes	:
T3652	6936-6937	CD	denotes	5
T3653	6939-6964	NN	denotes	CAGCCCAGGCAGGGTGCAGCACGAG
T3654	6937-6938	SYM	denotes	′
T3655	6938-6939	HYPH	denotes	-
T3656	6964-6965	HYPH	denotes	-
T3657	6965-6966	CD	denotes	3
T3658	6966-6967	SYM	denotes	′
T3659	6967-6968	:	denotes	;
T3660	6969-6970	CD	denotes	5
T3661	6972-6997	NN	denotes	CCGCCTCGTGCTGCACCCTGCCTGG
T3662	6970-6971	SYM	denotes	′
T3663	6971-6972	HYPH	denotes	-
T3664	6997-6998	HYPH	denotes	-
T3665	6998-6999	CD	denotes	3
T3666	6999-7000	SYM	denotes	′
T3667	7000-7002	,	denotes	,
T3668	7002-7007	NN	denotes	Ndrg1
T3669	7008-7012	NN	denotes	MRE2
T3670	7012-7014	:	denotes	:
T3671	7014-7015	CD	denotes	5
T3672	7017-7042	NN	denotes	CACACGTTCGCTGCACACGCCGCGG
T3673	7015-7016	SYM	denotes	′
T3674	7016-7017	HYPH	denotes	-
T3675	7042-7043	HYPH	denotes	-
T3676	7043-7044	CD	denotes	3
T3677	7044-7045	SYM	denotes	′
T3678	7045-7046	:	denotes	;
T3679	7047-7048	CD	denotes	5
T3680	7050-7075	NN	denotes	GGGACCGCGGCGTGTGCAGCGAACG
T3681	7048-7049	SYM	denotes	′
T3682	7049-7050	HYPH	denotes	-
T3683	7075-7076	HYPH	denotes	-
T3684	7076-7077	CD	denotes	3
T3685	7077-7078	SYM	denotes	′
T3686	7078-7080	,	denotes	,
T3687	7080-7085	NN	denotes	Ndrg1
T3688	7086-7090	NN	denotes	MRE3
T3689	7090-7091	,	denotes	,
T3690	7091-7092	CD	denotes	4
T3691	7092-7094	:	denotes	:
T3692	7094-7095	CD	denotes	5
T3693	7097-7136	NN	denotes	GGAGTCCTTATGCACACGCGCACGAGCGCGCACGGGCAC
T3694	7095-7096	SYM	denotes	′
T3695	7096-7097	HYPH	denotes	-
T3696	7136-7137	HYPH	denotes	-
T3697	7137-7138	CD	denotes	3
T3698	7138-7139	SYM	denotes	′
T3699	7139-7140	:	denotes	;
T3700	7141-7142	CD	denotes	5
T3701	7144-7184	NN	denotes	TGGTGTGCCCGTGCGCGCTCGTGCGCGTGTGCATAAGGAC
T3702	7142-7143	SYM	denotes	′
T3703	7143-7144	HYPH	denotes	-
T3704	7184-7185	HYPH	denotes	-
T3705	7185-7186	CD	denotes	3
T3706	7186-7187	SYM	denotes	′
T3707	7187-7189	,	denotes	,
T3708	7189-7194	NN	denotes	Sepw1
T3709	7195-7199	NN	denotes	MRE1
T3710	7199-7201	:	denotes	:
T3711	7201-7202	CD	denotes	5
T3712	7204-7237	NN	denotes	GAGGCAGTCGGCTGTGCGCACGGCCCCACGCTC
T3713	7202-7203	SYM	denotes	′
T3714	7203-7204	HYPH	denotes	-
T3715	7237-7238	HYPH	denotes	-
T3716	7238-7239	CD	denotes	3
T3717	7239-7240	SYM	denotes	′
T3718	7240-7241	:	denotes	;
T3719	7242-7243	CD	denotes	5
T3720	7245-7279	NN	denotes	CTCTGAGCGTGGGGCCGTGCGCACAGCCGACTGC
T3721	7243-7244	SYM	denotes	′
T3722	7244-7245	HYPH	denotes	-
T3723	7279-7280	HYPH	denotes	-
T3724	7280-7281	CD	denotes	3
T3725	7281-7282	SYM	denotes	′
T3726	7282-7284	,	denotes	,
T3727	7284-7289	NN	denotes	Sepw1
T3728	7290-7294	NN	denotes	MRE2
T3729	7294-7296	:	denotes	:
T3730	7296-7297	CD	denotes	5
T3731	7299-7327	NN	denotes	ATGGTTTTGGGGGTGCGCAGGGGGTCTG
T3732	7297-7298	SYM	denotes	′
T3733	7298-7299	HYPH	denotes	-
T3734	7327-7328	HYPH	denotes	-
T3735	7328-7329	CD	denotes	3
T3736	7329-7330	SYM	denotes	′
T3737	7330-7331	:	denotes	;
T3738	7332-7333	CD	denotes	5
T3739	7335-7363	NN	denotes	CGACAGACCCCCTGCGCACCCCCAAAAC
T3740	7333-7334	SYM	denotes	′
T3741	7334-7335	HYPH	denotes	-
T3742	7363-7364	HYPH	denotes	-
T3743	7364-7365	CD	denotes	3
T3744	7365-7366	SYM	denotes	′
T3745	7366-7368	,	denotes	,
T3746	7368-7376	NN	denotes	Slc39a10
T3747	7377-7381	NN	denotes	MRE1
T3748	7381-7383	:	denotes	:
T3749	7383-7384	CD	denotes	5
T3750	7386-7417	NN	denotes	GAATACACGACTGGGTGCAGCCGGGGTTTGG
T3751	7384-7385	SYM	denotes	′
T3752	7385-7386	HYPH	denotes	-
T3753	7417-7418	HYPH	denotes	-
T3754	7418-7419	CD	denotes	3
T3755	7419-7420	SYM	denotes	′
T3756	7420-7421	:	denotes	;
T3757	7422-7423	CD	denotes	5
T3758	7425-7457	NN	denotes	GGTACCAAACCCCGGCTGCACCCAGTCGTGTA
T3759	7423-7424	SYM	denotes	′
T3760	7424-7425	HYPH	denotes	-
T3761	7457-7458	HYPH	denotes	-
T3762	7458-7459	CD	denotes	3
T3763	7459-7460	SYM	denotes	′
T3764	7460-7462	,	denotes	,
T3765	7462-7470	NN	denotes	Slc39a10
T3766	7471-7475	NN	denotes	MRE2
T3767	7475-7477	:	denotes	:
T3768	7477-7478	CD	denotes	5
T3769	7480-7507	NN	denotes	GCGGAGAGGAGATGCACACGGCACTCG
T3770	7478-7479	SYM	denotes	′
T3771	7479-7480	HYPH	denotes	-
T3772	7507-7508	HYPH	denotes	-
T3773	7508-7509	CD	denotes	3
T3774	7509-7510	SYM	denotes	′
T3775	7510-7511	:	denotes	;
T3776	7512-7513	CD	denotes	5
T3777	7515-7542	NN	denotes	CACTCGAGTGCCGTGTGCATCTCCTCT
T3778	7513-7514	SYM	denotes	′
T3779	7514-7515	HYPH	denotes	-
T3780	7542-7543	HYPH	denotes	-
T3781	7543-7544	CD	denotes	3
T3782	7544-7545	SYM	denotes	′
T3783	7545-7547	,	denotes	,
T3784	7547-7558	NN	denotes	Specificity
T3785	7559-7566	NN	denotes	protein
T3786	7583-7591	NN	denotes	sequence
T3787	7567-7568	CD	denotes	1
T3788	7569-7570	-LRB-	denotes	(
T3789	7570-7573	NN	denotes	Sp1
T3790	7573-7574	-RRB-	denotes	)
T3791	7575-7582	NN	denotes	binding
T3792	7592-7593	-LRB-	denotes	(
T3793	7593-7595	CD	denotes	10
T3794	7595-7596	-RRB-	denotes	)
T3795	7596-7598	:	denotes	:
T3796	7598-7599	CD	denotes	5
T3797	7601-7616	NN	denotes	CGAGGCCCCGCCCAG
T3798	7599-7600	SYM	denotes	′
T3799	7600-7601	HYPH	denotes	-
T3800	7616-7617	HYPH	denotes	-
T3801	7617-7618	CD	denotes	3
T3802	7618-7619	SYM	denotes	′
T3803	7619-7620	:	denotes	;
T3804	7621-7622	CD	denotes	5
T3805	7624-7647	NN	denotes	TCGACTGGGCGGGGCCTCGAGct
T3806	7622-7623	SYM	denotes	′
T3807	7623-7624	HYPH	denotes	-
T3808	7647-7648	HYPH	denotes	-
T3809	7648-7649	CD	denotes	3
T3810	7649-7650	SYM	denotes	′
T3811	7650-7651	.	denotes	.
T3948	8010-8011	-LRB-	denotes	(
T3949	8011-8015	NN	denotes	SV40
T3950	8015-8016	-RRB-	denotes	)
T3951	8017-8022	JJ	denotes	large
T3952	8023-8024	NN	denotes	T
T3953	8033-8039	VBN	denotes	driven
T3954	8040-8042	IN	denotes	by
T3955	8043-8046	DT	denotes	the
T3956	8069-8077	NN	denotes	promoter
T3957	8047-8062	NN	denotes	cytomegalovirus
T3958	8063-8064	-LRB-	denotes	(
T3959	8064-8067	NN	denotes	CMV
T3960	8067-8068	-RRB-	denotes	)
T3961	8077-8079	,	denotes	,
T3962	8079-8084	VBG	denotes	using
T3963	8085-8098	NNP	denotes	lipofectamine
T3964	8100-8107	NN	denotes	reagent
T3965	8098-8099	SYM	denotes	™
T3966	8108-8109	-LRB-	denotes	(
T3967	8109-8119	NNP	denotes	Invitrogen
T3968	8119-8120	-RRB-	denotes	)
T3969	8121-8130	VBG	denotes	according
T3970	8131-8133	IN	denotes	to
T3971	8134-8137	DT	denotes	the
T3972	8138-8150	NN	denotes	manufacturer
T3973	8153-8165	NNS	denotes	instructions
T3974	8150-8152	POS	denotes	's
T3975	8165-8166	.	denotes	.
T3976	8166-8283	sentence	denotes	Cell foci were isolated and the immortalized mouse embryonic fibroblast cell line ckoC was derived from one of them.
T3977	8167-8171	NN	denotes	Cell
T3978	8172-8176	NNS	denotes	foci
T3979	8182-8190	VBN	denotes	isolated
T3980	8177-8181	VBD	denotes	were
T3981	8191-8194	CC	denotes	and
T3982	8195-8198	DT	denotes	the
T3983	8244-8248	NN	denotes	line
T3984	8199-8211	VBN	denotes	immortalized
T3985	8212-8217	NN	denotes	mouse
T3986	8228-8238	NN	denotes	fibroblast
T3987	8218-8227	JJ	denotes	embryonic
T3988	8239-8243	NN	denotes	cell
T3989	8258-8265	VBN	denotes	derived
T3990	8249-8253	NN	denotes	ckoC
T3991	8254-8257	VBD	denotes	was
T3992	8266-8270	IN	denotes	from
T3993	8271-8274	CD	denotes	one
T3994	8275-8277	IN	denotes	of
T3995	8278-8282	PRP	denotes	them
T3996	8282-8283	.	denotes	.
T3997	8283-8393	sentence	denotes	The Mtf1loxP genotype of this line as well as the genomic integration of the T antigen were confirmed by PCR.
T3998	8284-8287	DT	denotes	The
T3999	8297-8305	NN	denotes	genotype
T4000	8288-8296	NN	denotes	Mtf1loxP
T4001	8376-8385	VBN	denotes	confirmed
T4002	8306-8308	IN	denotes	of
T4003	8309-8313	DT	denotes	this
T4004	8314-8318	NN	denotes	line
T4005	8319-8321	RB	denotes	as
T4006	8327-8329	IN	denotes	as
T4007	8322-8326	RB	denotes	well
T4008	8330-8333	DT	denotes	the
T4009	8342-8353	NN	denotes	integration
T4010	8334-8341	JJ	denotes	genomic
T4011	8354-8356	IN	denotes	of
T4012	8357-8360	DT	denotes	the
T4013	8363-8370	NN	denotes	antigen
T4014	8361-8362	NN	denotes	T
T4015	8371-8375	VBD	denotes	were
T4016	8386-8388	IN	denotes	by
T4017	8389-8392	NN	denotes	PCR
T4018	8392-8393	.	denotes	.
T4019	8393-8678	sentence	denotes	100 mm plates with these cells were further transfected by the calcium phosphate method (30) with 19.6 µg of an expression plasmid for Cre recombinase driven by the CMV promoter and 0.4 µg of an expression plasmid for the neomycin resistance gene under the control of the TK promoter.
T4020	8394-8397	CD	denotes	100
T4021	8398-8400	NN	denotes	mm
T4022	8401-8407	NNS	denotes	plates
T4023	8438-8449	VBN	denotes	transfected
T4024	8408-8412	IN	denotes	with
T4025	8413-8418	DT	denotes	these
T4026	8419-8424	NNS	denotes	cells
T4027	8425-8429	VBD	denotes	were
T4028	8430-8437	RB	denotes	further
T4029	8450-8452	IN	denotes	by
T4030	8453-8456	DT	denotes	the
T4031	8475-8481	NN	denotes	method
T4032	8457-8464	NN	denotes	calcium
T4033	8465-8474	NN	denotes	phosphate
T4034	8482-8483	-LRB-	denotes	(
T4035	8483-8485	CD	denotes	30
T4036	8485-8486	-RRB-	denotes	)
T4037	8487-8491	IN	denotes	with
T4038	8492-8496	CD	denotes	19.6
T4039	8497-8499	NN	denotes	µg
T4040	8500-8502	IN	denotes	of
T4041	8503-8505	DT	denotes	an
T4042	8517-8524	NN	denotes	plasmid
T4043	8506-8516	NN	denotes	expression
T4044	8525-8528	IN	denotes	for
T4045	8529-8532	NN	denotes	Cre
T4046	8533-8544	NN	denotes	recombinase
T4047	8545-8551	VBN	denotes	driven
T4048	8552-8554	IN	denotes	by
T4049	8555-8558	DT	denotes	the
T4050	8563-8571	NN	denotes	promoter
T4051	8559-8562	NN	denotes	CMV
T4052	8572-8575	CC	denotes	and
T4053	8576-8579	CD	denotes	0.4
T4054	8580-8582	NN	denotes	µg
T4055	8583-8585	IN	denotes	of
T4056	8586-8588	DT	denotes	an
T4057	8600-8607	NN	denotes	plasmid
T4058	8589-8599	NN	denotes	expression
T4059	8608-8611	IN	denotes	for
T4060	8612-8615	DT	denotes	the
T4061	8636-8640	NN	denotes	gene
T4062	8616-8624	NN	denotes	neomycin
T4063	8625-8635	NN	denotes	resistance
T4064	8641-8646	IN	denotes	under
T4065	8647-8650	DT	denotes	the
T4066	8651-8658	NN	denotes	control
T4067	8659-8661	IN	denotes	of
T4068	8662-8665	DT	denotes	the
T4069	8669-8677	NN	denotes	promoter
T4070	8666-8668	NN	denotes	TK
T4071	8677-8678	.	denotes	.
T4072	8678-8941	sentence	denotes	Stably transfected cells were selected in the presence of 0.4 µg/µl G418 (Calbiochem), isolated clones of resistant cells were harvested and grown independently, and the expression of Cre recombinase and excision of exons 3 and 4 of Mtf1 were analyzed by RT–PCR.
T4073	8679-8685	RB	denotes	Stably
T4074	8686-8697	VBN	denotes	transfected
T4075	8698-8703	NNS	denotes	cells
T4076	8709-8717	VBN	denotes	selected
T4077	8704-8708	VBD	denotes	were
T4078	8718-8720	IN	denotes	in
T4079	8721-8724	DT	denotes	the
T4080	8725-8733	NN	denotes	presence
T4081	8734-8736	IN	denotes	of
T4082	8737-8740	CD	denotes	0.4
T4083	8741-8743	NN	denotes	µg
T4084	8747-8751	NN	denotes	G418
T4085	8743-8744	SYM	denotes	/
T4086	8744-8746	NN	denotes	µl
T4087	8752-8753	-LRB-	denotes	(
T4088	8753-8763	NNP	denotes	Calbiochem
T4089	8763-8764	-RRB-	denotes	)
T4090	8764-8766	,	denotes	,
T4091	8766-8774	VBN	denotes	isolated
T4092	8775-8781	NNS	denotes	clones
T4093	8806-8815	VBN	denotes	harvested
T4094	8782-8784	IN	denotes	of
T4095	8785-8794	JJ	denotes	resistant
T4096	8795-8800	NNS	denotes	cells
T4097	8801-8805	VBD	denotes	were
T4098	8816-8819	CC	denotes	and
T4099	8820-8825	VBN	denotes	grown
T4100	8826-8839	RB	denotes	independently
T4101	8839-8841	,	denotes	,
T4102	8841-8844	CC	denotes	and
T4103	8845-8848	DT	denotes	the
T4104	8849-8859	NN	denotes	expression
T4105	8922-8930	VBN	denotes	analyzed
T4106	8860-8862	IN	denotes	of
T4107	8863-8866	NN	denotes	Cre
T4108	8867-8878	NN	denotes	recombinase
T4109	8879-8882	CC	denotes	and
T4110	8883-8891	NN	denotes	excision
T4111	8892-8894	IN	denotes	of
T4112	8895-8900	NNS	denotes	exons
T4113	8901-8902	CD	denotes	3
T4114	8903-8906	CC	denotes	and
T4115	8907-8908	CD	denotes	4
T4116	8909-8911	IN	denotes	of
T4117	8912-8916	NN	denotes	Mtf1
T4118	8917-8921	VBD	denotes	were
T4119	8931-8933	IN	denotes	by
T4120	8934-8936	NN	denotes	RT
T4121	8937-8940	NN	denotes	PCR
T4122	8936-8937	HYPH	denotes	–
T4123	8940-8941	.	denotes	.
T4124	8941-9043	sentence	denotes	The cell lines delC19, delC21 and delC23 with a deletion of Mtf1 were chosen for further experiments.
T4125	8942-8945	DT	denotes	The
T4126	8951-8956	NNS	denotes	lines
T4127	8946-8950	NN	denotes	cell
T4128	9012-9018	VBN	denotes	chosen
T4129	8957-8963	NN	denotes	delC19
T4130	8963-8965	,	denotes	,
T4131	8965-8971	NN	denotes	delC21
T4132	8972-8975	CC	denotes	and
T4133	8976-8982	NN	denotes	delC23
T4134	8983-8987	IN	denotes	with
T4135	8988-8989	DT	denotes	a
T4136	8990-8998	NN	denotes	deletion
T4137	8999-9001	IN	denotes	of
T4138	9002-9006	NN	denotes	Mtf1
T4139	9007-9011	VBD	denotes	were
T4140	9019-9022	IN	denotes	for
T4141	9023-9030	JJ	denotes	further
T4142	9031-9042	NNS	denotes	experiments
T4143	9042-9043	.	denotes	.
T3137	4993-4995	NN	denotes	S1
T3138	5005-5012	NN	denotes	mapping
T3139	4996-5004	NN	denotes	nuclease
T3140	5013-5015	IN	denotes	of
T3141	5016-5027	NNS	denotes	transcripts
T3142	5028-5029	-LRB-	denotes	(
T3143	5032-5040	NN	denotes	analysis
T3144	5029-5031	NN	denotes	S1
T3145	5040-5041	-RRB-	denotes	)
T3146	5041-5178	sentence	denotes	S1 analysis was performed as previously described (29), using 100 µg DNase I-digested total RNA (RNA isolation see microarray analysis).
T3147	5042-5044	NN	denotes	S1
T3148	5045-5053	NN	denotes	analysis
T3149	5058-5067	VBN	denotes	performed
T3150	5054-5057	VBD	denotes	was
T3151	5068-5070	IN	denotes	as
T3152	5082-5091	VBN	denotes	described
T3153	5071-5081	RB	denotes	previously
T3154	5092-5093	-LRB-	denotes	(
T3155	5093-5095	CD	denotes	29
T3156	5095-5096	-RRB-	denotes	)
T3157	5096-5098	,	denotes	,
T3158	5098-5103	VBG	denotes	using
T3159	5104-5107	CD	denotes	100
T3160	5108-5110	NN	denotes	µg
T3161	5134-5137	NN	denotes	RNA
T3162	5111-5116	NN	denotes	DNase
T3163	5119-5127	VBN	denotes	digested
T3184	5225-5234	NNP	denotes	Molecular
T3196	5286-5289	NN	denotes	32P
T3164	5117-5118	CD	denotes	I
T3165	5118-5119	HYPH	denotes	-
T3166	5128-5133	JJ	denotes	total
T3167	5138-5139	-LRB-	denotes	(
T3168	5153-5156	VBP	denotes	see
T3169	5139-5142	NN	denotes	RNA
T3170	5143-5152	NN	denotes	isolation
T3171	5157-5167	NN	denotes	microarray
T3172	5168-5176	NN	denotes	analysis
T3173	5176-5177	-RRB-	denotes	)
T3174	5177-5178	.	denotes	.
T3175	5178-5245	sentence	denotes	The gels were developed using PhosphorImager (Molecular Dynamics).
T3176	5179-5182	DT	denotes	The
T3177	5183-5187	NNS	denotes	gels
T3178	5193-5202	VBN	denotes	developed
T3179	5188-5192	VBD	denotes	were
T3180	5203-5208	VBG	denotes	using
T3181	5209-5223	NNP	denotes	PhosphorImager
T3182	5224-5225	-LRB-	denotes	(
T3183	5235-5243	NNP	denotes	Dynamics
T3185	5243-5244	-RRB-	denotes	)
T3186	5244-5245	.	denotes	.
T3187	5245-5512	sentence	denotes	S1 analysis was done with the following 32P-labeled oligonucleotides: Hprt S1: 5′-TCTTCAGTCTGATAAAATCTACAGTCATAGGAATGGATCTATCACTATTTCTATTCAGTGATTACATTAAAG-3′, Sepw1 S1: 5′-TTCAACCGGGAACACCTGGAAACATGGCTGCCTGTCTTCTTGAAGTCTTGAGGTGGAAAGGGAAAGCAAAGCAGGAGGGTTTTCCCACCC-3′.
T3188	5246-5248	NN	denotes	S1
T3189	5249-5257	NN	denotes	analysis
T3190	5262-5266	VBN	denotes	done
T3191	5258-5261	VBD	denotes	was
T3192	5267-5271	IN	denotes	with
T3193	5272-5275	DT	denotes	the
T3194	5298-5314	NNS	denotes	oligonucleotides
T3195	5276-5285	VBG	denotes	following
T3197	5290-5297	VBN	denotes	labeled
T3198	5289-5290	HYPH	denotes	-
T3199	5314-5316	:	denotes	:
T3200	5316-5320	NN	denotes	Hprt
T3201	5321-5323	NN	denotes	S1
T3202	5323-5325	:	denotes	:
T3203	5325-5326	CD	denotes	5
T3204	5328-5400	NN	denotes	TCTTCAGTCTGATAAAATCTACAGTCATAGGAATGGATCTATCACTATTTCTATTCAGTGATTACATTAAAG
T3205	5326-5327	SYM	denotes	′
T3206	5327-5328	HYPH	denotes	-
T3207	5400-5401	HYPH	denotes	-
T3208	5401-5402	CD	denotes	3
T3209	5402-5403	SYM	denotes	′
T3210	5403-5405	,	denotes	,
T3211	5405-5410	NN	denotes	Sepw1
T3212	5411-5413	NN	denotes	S1
T3213	5413-5415	:	denotes	:
T3214	5415-5416	CD	denotes	5
T3215	5418-5508	NN	denotes	TTCAACCGGGAACACCTGGAAACATGGCTGCCTGTCTTCTTGAAGTCTTGAGGTGGAAAGGGAAAGCAAAGCAGGAGGGTTTTCCCACCC
T3216	5416-5417	SYM	denotes	′
T3217	5417-5418	HYPH	denotes	-
T3218	5508-5509	HYPH	denotes	-
T3219	5509-5510	CD	denotes	3
T3220	5510-5511	SYM	denotes	′
T3221	5511-5512	.	denotes	.
T4279	9045-9057	NN	denotes	Cytotoxicity
T4280	9058-9063	NN	denotes	assay
T4281	9063-9170	sentence	denotes	Samples of 1 × 104 cells/well were plated in 96-well tissue culture plates and allowed to adhere for 24 h.
T4282	9064-9071	NNS	denotes	Samples
T4283	9099-9105	VBN	denotes	plated
T4284	9072-9074	IN	denotes	of
T4285	9075-9076	CD	denotes	1
T4286	9079-9082	CD	denotes	104
T4287	9077-9078	SYM	denotes	×
T4288	9083-9088	NNS	denotes	cells
T4289	9088-9089	SYM	denotes	/
T4290	9089-9093	NN	denotes	well
T4291	9094-9098	VBD	denotes	were
T4292	9106-9108	IN	denotes	in
T4293	9109-9111	CD	denotes	96
T4294	9112-9116	NN	denotes	well
T4295	9111-9112	HYPH	denotes	-
T4296	9132-9138	NNS	denotes	plates
T4297	9117-9123	NN	denotes	tissue
T4298	9124-9131	NN	denotes	culture
T4299	9139-9142	CC	denotes	and
T4300	9143-9150	VBN	denotes	allowed
T4301	9151-9153	TO	denotes	to
T4302	9154-9160	VB	denotes	adhere
T4303	9161-9164	IN	denotes	for
T4304	9165-9167	CD	denotes	24
T4305	9168-9169	NN	denotes	h
T4306	9169-9170	.	denotes	.
T4307	9170-9351	sentence	denotes	The cells were then pre-incubated for 24 h in medium containing 0, 5, 10, 25 or 50 µM l-buthionine-[S,R]-sulfoximine (BSO) (Sigma), a drug that inhibits glutathione synthesis (31).
T4308	9171-9174	DT	denotes	The
T4309	9175-9180	NNS	denotes	cells
T4310	9191-9204	VBN	denotes	pre-incubated
T4311	9181-9185	VBD	denotes	were
T4312	9186-9190	RB	denotes	then
T4313	9205-9208	IN	denotes	for
T4314	9209-9211	CD	denotes	24
T4315	9212-9213	NN	denotes	h
T4316	9214-9216	IN	denotes	in
T4317	9217-9223	NN	denotes	medium
T4318	9224-9234	VBG	denotes	containing
T4319	9235-9236	CD	denotes	0
T4320	9254-9256	NN	denotes	µM
T4321	9236-9238	,	denotes	,
T4322	9238-9239	CD	denotes	5
T4323	9239-9241	,	denotes	,
T4324	9241-9243	CD	denotes	10
T4325	9243-9245	,	denotes	,
T4326	9245-9247	CD	denotes	25
T4327	9248-9250	CC	denotes	or
T4328	9251-9253	CD	denotes	50
T4329	9257-9258	NN	denotes	l
T4330	9276-9287	NN	denotes	sulfoximine
T4331	9258-9259	HYPH	denotes	-
T4332	9259-9269	NN	denotes	buthionine
T4333	9269-9270	HYPH	denotes	-
T4334	9270-9271	-LRB-	denotes	[
T4335	9271-9272	NN	denotes	S
T4336	9272-9273	,	denotes	,
T4337	9273-9274	NN	denotes	R
T4338	9274-9275	-RRB-	denotes	]
T4339	9275-9276	HYPH	denotes	-
T4340	9288-9289	-LRB-	denotes	(
T4341	9289-9292	NN	denotes	BSO
T4342	9292-9293	-RRB-	denotes	)
T4343	9294-9295	-LRB-	denotes	(
T4344	9295-9300	NNP	denotes	Sigma
T4345	9300-9301	-RRB-	denotes	)
T4346	9301-9303	,	denotes	,
T4347	9303-9304	DT	denotes	a
T4348	9305-9309	NN	denotes	drug
T4349	9310-9314	WDT	denotes	that
T4350	9315-9323	VBZ	denotes	inhibits
T4351	9324-9335	NN	denotes	glutathione
T4352	9336-9345	NN	denotes	synthesis
T4353	9346-9347	-LRB-	denotes	(
T4354	9347-9349	CD	denotes	31
T4355	9349-9350	-RRB-	denotes	)
T4356	9350-9351	.	denotes	.
T4357	9351-9467	sentence	denotes	Later, cells were exposed to 0, 5, 10 or 20 µM CdCl2 in the specified pre-incubation medium for an additional 24 h.
T4358	9352-9357	RB	denotes	Later
T4359	9370-9377	VBN	denotes	exposed
T4360	9357-9359	,	denotes	,
T4361	9359-9364	NNS	denotes	cells
T4362	9365-9369	VBD	denotes	were
T4363	9378-9380	IN	denotes	to
T4364	9381-9382	CD	denotes	0
T4365	9396-9398	NN	denotes	µM
T4366	9382-9384	,	denotes	,
T4367	9384-9385	CD	denotes	5
T4368	9385-9387	,	denotes	,
T4369	9387-9389	CD	denotes	10
T4370	9390-9392	CC	denotes	or
T4371	9393-9395	CD	denotes	20
T4372	9399-9404	NN	denotes	CdCl2
T4373	9405-9407	IN	denotes	in
T4374	9408-9411	DT	denotes	the
T4375	9437-9443	NN	denotes	medium
T4376	9412-9421	VBN	denotes	specified
T4377	9422-9436	NN	denotes	pre-incubation
T4378	9444-9447	IN	denotes	for
T4379	9448-9450	DT	denotes	an
T4380	9465-9466	NN	denotes	h
T4381	9451-9461	JJ	denotes	additional
T4382	9462-9464	CD	denotes	24
T4383	9466-9467	.	denotes	.
T2931	4558-4579	NN	denotes	TTCCGATGTGCCAAGTGTGGC
T4384	9467-9654	sentence	denotes	Cytotoxicity was determined by the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromid)-based Cell Proliferation Kit I (Roche) according to the manufacturer's instructions.
T4385	9468-9480	NN	denotes	Cytotoxicity
T4386	9485-9495	VBN	denotes	determined
T4387	9481-9484	VBD	denotes	was
T4388	9496-9498	IN	denotes	by
T4389	9499-9502	DT	denotes	the
T4390	9595-9598	NNP	denotes	Kit
T4391	9503-9506	NN	denotes	MTT
T4392	9570-9575	VBN	denotes	based
T4393	9507-9508	-LRB-	denotes	(
T4394	9562-9568	NN	denotes	bromid
T4395	9508-9509	CD	denotes	3
T4396	9515-9530	NN	denotes	dimethylthiazol
T4397	9509-9510	HYPH	denotes	-
T4398	9510-9511	-LRB-	denotes	[
T4399	9511-9512	CD	denotes	4
T4400	9512-9513	,	denotes	,
T4401	9513-9514	CD	denotes	5
T4402	9514-9515	HYPH	denotes	-
T4403	9530-9531	HYPH	denotes	-
T4404	9531-9532	CD	denotes	2
T4405	9533-9535	NN	denotes	yl
T4406	9532-9533	HYPH	denotes	-
T4407	9535-9536	-RRB-	denotes	]
T4408	9536-9537	HYPH	denotes	-
T4409	9537-9538	CD	denotes	2
T4410	9538-9539	,	denotes	,
T4411	9539-9540	CD	denotes	5
T4412	9541-9549	NN	denotes	diphenyl
T4413	9540-9541	HYPH	denotes	-
T4414	9550-9561	NN	denotes	tetrazolium
T4415	9568-9569	-RRB-	denotes	)
T4416	9569-9570	HYPH	denotes	-
T4417	9576-9580	NNP	denotes	Cell
T4418	9581-9594	NNP	denotes	Proliferation
T4419	9599-9600	CD	denotes	I
T4420	9601-9602	-LRB-	denotes	(
T4421	9602-9607	NNP	denotes	Roche
T4422	9607-9608	-RRB-	denotes	)
T4423	9609-9618	VBG	denotes	according
T4424	9619-9621	IN	denotes	to
T4425	9622-9625	DT	denotes	the
T4426	9626-9638	NN	denotes	manufacturer
T4427	9641-9653	NNS	denotes	instructions
T4428	9638-9640	POS	denotes	's
T4429	9653-9654	.	denotes	.
T2932	4282-4284	NN	denotes	RT
T2933	4285-4288	NN	denotes	PCR
T2934	4284-4285	HYPH	denotes	–
T2935	4288-4487	sentence	denotes	All RT–PCRs were performed with QIAGEN® OneStep RT–PCR Kit (QIAGEN) according to the manufacturer's instructions, using 150–200 ng DNase I-digested total RNA (RNA isolation see microarray analysis).
T2936	4289-4292	DT	denotes	All
T2937	4296-4300	NNS	denotes	PCRs
T2938	4293-4295	NN	denotes	RT
T2939	4295-4296	HYPH	denotes	–
T2940	4306-4315	VBN	denotes	performed
T2941	4301-4305	VBD	denotes	were
T2942	4316-4320	IN	denotes	with
T2943	4321-4328	NNP	denotes	QIAGEN®
T2944	4344-4347	NN	denotes	Kit
T2945	4329-4336	NNP	denotes	OneStep
T2946	4337-4339	NN	denotes	RT
T2947	4340-4343	NN	denotes	PCR
T2948	4339-4340	HYPH	denotes	–
T2949	4348-4349	-LRB-	denotes	(
T2950	4349-4355	NNP	denotes	QIAGEN
T2951	4355-4356	-RRB-	denotes	)
T2952	4357-4366	VBG	denotes	according
T2953	4367-4369	IN	denotes	to
T2954	4370-4373	DT	denotes	the
T2955	4374-4386	NN	denotes	manufacturer
T2956	4389-4401	NNS	denotes	instructions
T2957	4386-4388	POS	denotes	's
T2958	4401-4403	,	denotes	,
T2959	4403-4408	VBG	denotes	using
T2960	4409-4412	CD	denotes	150
T2961	4413-4416	CD	denotes	200
T2962	4412-4413	SYM	denotes	–
T2963	4417-4419	NN	denotes	ng
T2964	4443-4446	NN	denotes	RNA
T2965	4420-4425	NN	denotes	DNase
T2966	4426-4427	NN	denotes	I
T2967	4428-4436	VBN	denotes	digested
T2968	4427-4428	HYPH	denotes	-
T2969	4437-4442	JJ	denotes	total
T2970	4447-4448	-LRB-	denotes	(
T2971	4452-4461	NN	denotes	isolation
T2972	4448-4451	NN	denotes	RNA
T2973	4462-4465	VBP	denotes	see
T2974	4466-4476	NN	denotes	microarray
T2975	4477-4485	NN	denotes	analysis
T2976	4485-4486	-RRB-	denotes	)
T2977	4486-4487	.	denotes	.
T2978	4487-4991	sentence	denotes	The reactions were carried out using the following primers: Csrp1: 5′-TTCCGATGTGCCAAGTGTGGC-3′; 5′-AGTAGAGAGTGGACATTCAGC-3′, hypoxanthin-guanin-phosphoribosyltransferase (Hprt): 5′-GCTGGTGAAAAGGACCTCTCG-3′; 5′-CCACAGGACTAGGACACCTGC-3′, Mtf1: 5′-GTGACTTTTGAGACTGTACTGAGTG-3′; 5′-CATGCCAAGAAACATTGAAGGTG-3′, Ndrg1: 5′-AGATACACAACAACGTGGAGG-3′; 5′-TGTGCGAGCGGCTTCGGGGGC-3′, Sepw1: 5′-TAGAGGCAGGGTCCTGAAAGC-3′; 5′-ACACCTGGAAACATGGCTGCC-3′, Slc39a10: 5′-GCTGTGGCTGGTAGTAAAAGC-3′; 5′-GTGGCATGGGATGTAAACAGC-3′.
T2979	4488-4491	DT	denotes	The
T2980	4492-4501	NNS	denotes	reactions
T2981	4507-4514	VBN	denotes	carried
T2982	4502-4506	VBD	denotes	were
T2983	4515-4518	RP	denotes	out
T2984	4519-4524	VBG	denotes	using
T2985	4525-4528	DT	denotes	the
T2986	4539-4546	NNS	denotes	primers
T2987	4529-4538	VBG	denotes	following
T2988	4546-4548	:	denotes	:
T2989	4548-4553	NN	denotes	Csrp1
T2990	4553-4555	:	denotes	:
T2991	4555-4556	CD	denotes	5
T2992	4556-4557	SYM	denotes	′
T2993	4557-4558	HYPH	denotes	-
T2994	4579-4580	HYPH	denotes	-
T2995	4580-4581	CD	denotes	3
T2996	4581-4582	SYM	denotes	′
T2997	4582-4583	:	denotes	;
T2998	4584-4585	CD	denotes	5
T2999	4587-4608	NN	denotes	AGTAGAGAGTGGACATTCAGC
T3000	4585-4586	SYM	denotes	′
T3001	4586-4587	HYPH	denotes	-
T3002	4608-4609	HYPH	denotes	-
T3003	4609-4610	CD	denotes	3
T3004	4610-4611	SYM	denotes	′
T3005	4611-4613	,	denotes	,
T3006	4613-4624	NN	denotes	hypoxanthin
T3007	4632-4657	NN	denotes	phosphoribosyltransferase
T3008	4624-4625	HYPH	denotes	-
T3009	4625-4631	NN	denotes	guanin
T3010	4631-4632	HYPH	denotes	-
T3011	4658-4659	-LRB-	denotes	(
T3012	4659-4663	NN	denotes	Hprt
T3013	4663-4664	-RRB-	denotes	)
T3014	4664-4666	:	denotes	:
T3015	4666-4667	CD	denotes	5
T3016	4669-4690	NN	denotes	GCTGGTGAAAAGGACCTCTCG
T3017	4667-4668	SYM	denotes	′
T3018	4668-4669	HYPH	denotes	-
T3019	4690-4691	HYPH	denotes	-
T3020	4691-4692	CD	denotes	3
T3021	4692-4693	SYM	denotes	′
T3022	4693-4694	:	denotes	;
T3023	4695-4696	CD	denotes	5
T3024	4698-4719	NN	denotes	CCACAGGACTAGGACACCTGC
T3027	4719-4720	HYPH	denotes	-
T3028	4720-4721	CD	denotes	3
T3029	4721-4722	SYM	denotes	′
T3030	4722-4724	,	denotes	,
T3031	4724-4728	NN	denotes	Mtf1
T3032	4728-4730	:	denotes	:
T3033	4730-4731	CD	denotes	5
T3034	4733-4758	NN	denotes	GTGACTTTTGAGACTGTACTGAGTG
T3035	4731-4732	SYM	denotes	′
T3036	4732-4733	HYPH	denotes	-
T3037	4758-4759	HYPH	denotes	-
T3038	4759-4760	CD	denotes	3
T3039	4760-4761	SYM	denotes	′
T3040	4761-4762	:	denotes	;
T3041	4763-4764	CD	denotes	5
T3042	4766-4789	NN	denotes	CATGCCAAGAAACATTGAAGGTG
T3043	4764-4765	SYM	denotes	′
T3044	4765-4766	HYPH	denotes	-
T3045	4789-4790	HYPH	denotes	-
T3046	4790-4791	CD	denotes	3
T3047	4791-4792	SYM	denotes	′
T3048	4792-4794	,	denotes	,
T2279	1964-1968	IN	denotes	from
T3049	4794-4799	NN	denotes	Ndrg1
T3050	4799-4801	:	denotes	:
T3051	4801-4802	CD	denotes	5
T3052	4804-4825	NN	denotes	AGATACACAACAACGTGGAGG
T3053	4802-4803	SYM	denotes	′
T2293	2031-2033	NN	denotes	pC
T3054	4803-4804	HYPH	denotes	-
T3055	4825-4826	HYPH	denotes	-
T3056	4826-4827	CD	denotes	3
T3057	4827-4828	SYM	denotes	′
T3058	4828-4829	:	denotes	;
T3059	4830-4831	CD	denotes	5
T3060	4833-4854	NN	denotes	TGTGCGAGCGGCTTCGGGGGC
T3061	4831-4832	SYM	denotes	′
T3062	4832-4833	HYPH	denotes	-
T3063	4854-4855	HYPH	denotes	-
T3064	4855-4856	CD	denotes	3
T3065	4856-4857	SYM	denotes	′
T3066	4857-4859	,	denotes	,
T3067	4859-4864	NN	denotes	Sepw1
T3068	4864-4866	:	denotes	:
T3069	4866-4867	CD	denotes	5
T3070	4869-4890	NN	denotes	TAGAGGCAGGGTCCTGAAAGC
T3071	4867-4868	SYM	denotes	′
T3072	4868-4869	HYPH	denotes	-
T3073	4890-4891	HYPH	denotes	-
T3074	4891-4892	CD	denotes	3
T3075	4892-4893	SYM	denotes	′
T3076	4893-4894	:	denotes	;
T3077	4895-4896	CD	denotes	5
T3078	4898-4919	NN	denotes	ACACCTGGAAACATGGCTGCC
T3079	4896-4897	SYM	denotes	′
T3080	4897-4898	HYPH	denotes	-
T3081	4919-4920	HYPH	denotes	-
T3082	4920-4921	CD	denotes	3
T3083	4921-4922	SYM	denotes	′
T3084	4922-4924	,	denotes	,
T3085	4924-4932	NN	denotes	Slc39a10
T3086	4932-4934	:	denotes	:
T3087	4934-4935	CD	denotes	5
T3088	4937-4958	NN	denotes	GCTGTGGCTGGTAGTAAAAGC
T3089	4935-4936	SYM	denotes	′
T3090	4936-4937	HYPH	denotes	-
T3091	4958-4959	HYPH	denotes	-
T3092	4959-4960	CD	denotes	3
T3093	4960-4961	SYM	denotes	′
T3094	4961-4962	:	denotes	;
T3095	4963-4964	CD	denotes	5
T3096	4966-4987	NN	denotes	GTGGCATGGGATGTAAACAGC
T3097	4964-4965	SYM	denotes	′
T3098	4965-4966	HYPH	denotes	-
T3099	4987-4988	HYPH	denotes	-
T3100	4988-4989	CD	denotes	3
T3101	4989-4990	SYM	denotes	′
T3102	4990-4991	.	denotes	.
T2173	1490-1496	NN	denotes	Animal
T2174	1497-1506	NN	denotes	treatment
T2175	1506-1904	sentence	denotes	At 8 weeks of age, male Mtf1loxP/loxP mice harboring the Mx-cre transgene (Mtf1loxP/loxP Mx-cre, abbr.: Mtf1Mx-cre) and control littermates without transgene (Mtf1loxP/loxP, abbr.: Mtf1loxP) received four intraperitoneal injections each of 300 µg synthetic double-stranded RNA polyinosinic–polycytidylic acid [pI–pC; Sigma; in a volume of 60 µl phosphate-buffered saline (PBS)] at 3 day intervals.
T2176	1507-1509	IN	denotes	At
T2177	1698-1706	VBD	denotes	received
T2178	1510-1511	CD	denotes	8
T2179	1512-1517	NNS	denotes	weeks
T2180	1518-1520	IN	denotes	of
T2181	1521-1524	NN	denotes	age
T2182	1524-1526	,	denotes	,
T2183	1526-1530	JJ	denotes	male
T2184	1545-1549	NNS	denotes	mice
T2185	1531-1539	NN	denotes	Mtf1loxP
T2186	1540-1544	NN	denotes	loxP
T2187	1539-1540	HYPH	denotes	/
T2188	1550-1559	VBG	denotes	harboring
T2189	1560-1563	DT	denotes	the
T2190	1571-1580	NN	denotes	transgene
T2191	1564-1566	NN	denotes	Mx
T2192	1567-1570	NN	denotes	cre
T2193	1566-1567	HYPH	denotes	-
T2194	1581-1582	-LRB-	denotes	(
T2195	1582-1590	NN	denotes	Mtf1loxP
T2196	1599-1602	NN	denotes	cre
T2197	1590-1591	HYPH	denotes	/
T2198	1591-1595	NN	denotes	loxP
T2199	1596-1598	NN	denotes	Mx
T2200	1598-1599	HYPH	denotes	-
T2201	1602-1604	,	denotes	,
T2202	1604-1609	RB	denotes	abbr.
T2203	1618-1621	NN	denotes	cre
T2204	1609-1611	:	denotes	:
T2205	1611-1617	NN	denotes	Mtf1Mx
T2206	1617-1618	HYPH	denotes	-
T2207	1621-1622	-RRB-	denotes	)
T2208	1623-1626	CC	denotes	and
T2209	1627-1634	NN	denotes	control
T2210	1635-1646	NNS	denotes	littermates
T2211	1647-1654	IN	denotes	without
T2212	1655-1664	NN	denotes	transgene
T2213	1665-1666	-LRB-	denotes	(
T2214	1666-1674	NN	denotes	Mtf1loxP
T2215	1675-1679	NN	denotes	loxP
T2216	1674-1675	HYPH	denotes	/
T2217	1679-1681	,	denotes	,
T2218	1681-1686	NN	denotes	abbr.
T2219	1688-1696	NN	denotes	Mtf1loxP
T2220	1686-1688	:	denotes	:
T2221	1696-1697	-RRB-	denotes	)
T2222	1707-1711	CD	denotes	four
T2223	1728-1738	NNS	denotes	injections
T2224	1712-1727	JJ	denotes	intraperitoneal
T2225	1739-1743	RB	denotes	each
T2226	1744-1746	IN	denotes	of
T2227	1747-1750	CD	denotes	300
T2228	1751-1753	NN	denotes	µg
T2229	1811-1815	NN	denotes	acid
T2230	1754-1763	JJ	denotes	synthetic
T2231	1764-1770	JJ	denotes	double
T2232	1771-1779	VBN	denotes	stranded
T2233	2178-2180	CD	denotes	20
T2234	1770-1771	HYPH	denotes	-
T2235	1780-1783	NN	denotes	RNA
T2236	1784-1796	JJ	denotes	polyinosinic
T2237	1797-1810	JJ	denotes	polycytidylic
T3873	7730-7738	NN	denotes	Mtf1loxP
T2238	1796-1797	HYPH	denotes	–
T2239	1816-1817	-LRB-	denotes	[
T2240	1824-1829	NNP	denotes	Sigma
T2241	1817-1819	NN	denotes	pI
T3874	7838-7850	NN	denotes	streptomycin
T2242	1820-1822	NN	denotes	pC
T2243	1819-1820	HYPH	denotes	–
T2244	1822-1823	:	denotes	;
T2245	1829-1830	:	denotes	;
T2246	1831-1833	IN	denotes	in
T2247	1834-1835	DT	denotes	a
T2248	1836-1842	NN	denotes	volume
T2249	1843-1845	IN	denotes	of
T2250	1846-1848	CD	denotes	60
T2251	1849-1851	NN	denotes	µl
T2252	1852-1861	NN	denotes	phosphate
T2253	1862-1870	VBN	denotes	buffered
T2254	1861-1862	HYPH	denotes	-
T2255	1871-1877	NN	denotes	saline
T2256	1878-1879	-LRB-	denotes	(
T2257	1879-1882	NN	denotes	PBS
T2258	1882-1883	-RRB-	denotes	)
T2259	1883-1884	-RRB-	denotes	]
T2260	1885-1887	IN	denotes	at
T2261	1888-1889	CD	denotes	3
T2262	1890-1893	NN	denotes	day
T2263	1894-1903	NNS	denotes	intervals
T2264	1903-1904	.	denotes	.
T2265	1904-2045	sentence	denotes	Only in the case of DNA-binding studies with MRE sequences from MTF-1 target gene candidates, the control mice received no pI–pC injections.
T2266	1905-1909	RB	denotes	Only
T2267	1910-1912	IN	denotes	in
T2268	2016-2024	VBD	denotes	received
T2269	1913-1916	DT	denotes	the
T2270	1917-1921	NN	denotes	case
T2271	1922-1924	IN	denotes	of
T2272	1925-1928	NN	denotes	DNA
T2273	1929-1936	VBG	denotes	binding
T2274	1928-1929	HYPH	denotes	-
T2275	1937-1944	NNS	denotes	studies
T2276	1945-1949	IN	denotes	with
T2277	1950-1953	NN	denotes	MRE
T2278	1954-1963	NNS	denotes	sequences
T2280	1969-1972	NN	denotes	MTF
T2281	1987-1997	NNS	denotes	candidates
T2282	1972-1973	HYPH	denotes	-
T2283	1973-1974	CD	denotes	1
T2284	1975-1981	NN	denotes	target
T2285	1982-1986	NN	denotes	gene
T2286	1997-1999	,	denotes	,
T2287	1999-2002	DT	denotes	the
T2288	2011-2015	NNS	denotes	mice
T2289	2003-2010	NN	denotes	control
T2290	2025-2027	DT	denotes	no
T2291	2034-2044	NNS	denotes	injections
T2292	2028-2030	NN	denotes	pI
T2294	2030-2031	HYPH	denotes	–
T2295	2044-2045	.	denotes	.
T2296	2045-2319	sentence	denotes	For experiments with metal treatment, mice received 2 days after the last pI–pC treatment a subcutaneous (s.c.) injection of either 20 µmol/kg body weight CdSO4 (2 mM CdSO4 in H2O; cadmium treatment) or 10 ml/kg body weight H2O (mock treatment) 6 h before sacrificing them.
T2297	2046-2049	IN	denotes	For
T2298	2089-2097	VBD	denotes	received
T2299	2050-2061	NNS	denotes	experiments
T2300	2062-2066	IN	denotes	with
T2301	2067-2072	NN	denotes	metal
T2302	2073-2082	NN	denotes	treatment
T2303	2082-2084	,	denotes	,
T2304	2084-2088	NNS	denotes	mice
T2305	2098-2099	CD	denotes	2
T2306	2100-2104	NNS	denotes	days
T2307	2105-2110	IN	denotes	after
T2308	2111-2114	DT	denotes	the
T2309	2126-2135	NN	denotes	treatment
T2310	2115-2119	JJ	denotes	last
T2311	2120-2122	NN	denotes	pI
T2312	2123-2125	NN	denotes	pC
T2313	2122-2123	HYPH	denotes	–
T2314	2136-2137	DT	denotes	a
T2315	2158-2167	NN	denotes	injection
T2316	2138-2150	JJ	denotes	subcutaneous
T2317	2152-2156	JJ	denotes	s.c.
T2318	2151-2152	-LRB-	denotes	(
T2319	2156-2157	-RRB-	denotes	)
T2320	2168-2170	IN	denotes	of
T2321	2171-2177	CC	denotes	either
T2322	2201-2206	NN	denotes	CdSO4
T2323	2181-2185	NN	denotes	µmol
T2324	2185-2186	SYM	denotes	/
T2325	2194-2200	NN	denotes	weight
T2326	2186-2188	NN	denotes	kg
T2327	2189-2193	NN	denotes	body
T2328	2207-2208	-LRB-	denotes	(
T2329	2235-2244	NN	denotes	treatment
T2330	2208-2209	CD	denotes	2
T2331	2210-2212	NN	denotes	mM
T2334	2222-2225	NN	denotes	H2O
T2332	2213-2218	NN	denotes	CdSO4
T2333	2219-2221	IN	denotes	in
T2335	2225-2226	:	denotes	;
T2336	2227-2234	NN	denotes	cadmium
T2337	2244-2245	-RRB-	denotes	)
T2338	2246-2248	CC	denotes	or
T2339	2249-2251	CD	denotes	10
T2340	2252-2254	NN	denotes	ml
T2341	2270-2273	NN	denotes	H2O
T2342	2254-2255	SYM	denotes	/
T2343	2263-2269	NN	denotes	weight
T2344	2255-2257	NN	denotes	kg
T2345	2258-2262	NN	denotes	body
T2346	2274-2275	-LRB-	denotes	(
T2347	2280-2289	NN	denotes	treatment
T2348	2275-2279	JJ	denotes	mock
T2349	2289-2290	-RRB-	denotes	)
T2350	2291-2292	CD	denotes	6
T2351	2293-2294	NN	denotes	h
T2352	2295-2301	IN	denotes	before
T2353	2302-2313	VBG	denotes	sacrificing
T2354	2314-2318	PRP	denotes	them
T2355	2318-2319	.	denotes	.
T3875	7653-7657	NN	denotes	Cell
T3876	7658-7665	NN	denotes	culture
T3877	7665-7896	sentence	denotes	Primary embryonic fibroblasts were isolated from a 12.5 day old Mtf1loxP mouse embryo and grown in DMEM supplemented with 10% fetal bovine serum (ICN), 100 U/ml penicillin–streptomycin (Gibco BRL) and 2 mM l-glutamine (Gibco BRL).
T3878	7666-7673	JJ	denotes	Primary
T3879	7684-7695	NNS	denotes	fibroblasts
T3880	7674-7683	JJ	denotes	embryonic
T3881	7701-7709	VBN	denotes	isolated
T3882	7696-7700	VBD	denotes	were
T3883	7710-7714	IN	denotes	from
T3884	7715-7716	DT	denotes	a
T3885	7722-7725	NN	denotes	day
T3886	7717-7721	CD	denotes	12.5
T3887	7726-7729	JJ	denotes	old
T3888	7745-7751	NN	denotes	embryo
T3889	7739-7744	NN	denotes	mouse
T3890	7752-7755	CC	denotes	and
T3891	7756-7761	VBN	denotes	grown
T3892	7762-7764	IN	denotes	in
T3893	7765-7769	NN	denotes	DMEM
T3894	7770-7782	VBN	denotes	supplemented
T3895	7783-7787	IN	denotes	with
T3896	7788-7790	CD	denotes	10
T3897	7790-7791	NN	denotes	%
T3898	7805-7810	NN	denotes	serum
T3899	7792-7797	JJ	denotes	fetal
T3900	7798-7804	JJ	denotes	bovine
T3901	7811-7812	-LRB-	denotes	(
T3902	7812-7815	NN	denotes	ICN
T3903	7815-7816	-RRB-	denotes	)
T3904	7816-7818	,	denotes	,
T3905	7818-7821	CD	denotes	100
T3906	7822-7823	NN	denotes	U
T3907	7823-7824	SYM	denotes	/
T3908	7824-7826	NN	denotes	ml
T3909	7827-7837	NN	denotes	penicillin
T3910	7837-7838	HYPH	denotes	–
T3911	7851-7852	-LRB-	denotes	(
T3912	7858-7861	NNP	denotes	BRL
T3913	7852-7857	NNP	denotes	Gibco
T3914	7861-7862	-RRB-	denotes	)
T3915	7863-7866	CC	denotes	and
T3916	7867-7868	CD	denotes	2
T3917	7869-7871	NN	denotes	mM
T3918	7874-7883	NN	denotes	glutamine
T3919	7872-7873	NN	denotes	l
T3920	7873-7874	HYPH	denotes	-
T3921	7884-7885	-LRB-	denotes	(
T3922	7891-7894	NNP	denotes	BRL
T3923	7885-7890	NNP	denotes	Gibco
T3924	7894-7895	-RRB-	denotes	)
T3925	7895-7896	.	denotes	.
T3926	7896-8166	sentence	denotes	100 mm plates with primary cells were transfected with 10 µg of an expression plasmid coding for simian virus 40 (SV40) large T antigen driven by the cytomegalovirus (CMV) promoter, using lipofectamine™ reagent (Invitrogen) according to the manufacturer's instructions.
T3927	7897-7900	CD	denotes	100
T3928	7901-7903	NN	denotes	mm
T3929	7904-7910	NNS	denotes	plates
T3930	7935-7946	VBN	denotes	transfected
T3931	7911-7915	IN	denotes	with
T3932	7916-7923	JJ	denotes	primary
T3933	7924-7929	NNS	denotes	cells
T3934	7930-7934	VBD	denotes	were
T3935	7947-7951	IN	denotes	with
T3936	7952-7954	CD	denotes	10
T3937	7955-7957	NN	denotes	µg
T3938	7958-7960	IN	denotes	of
T3939	7961-7963	DT	denotes	an
T3940	7975-7982	NN	denotes	plasmid
T3941	7964-7974	NN	denotes	expression
T3942	7983-7989	VBG	denotes	coding
T3943	7990-7993	IN	denotes	for
T3944	7994-8000	JJ	denotes	simian
T3945	8001-8006	NN	denotes	virus
T3946	8025-8032	NN	denotes	antigen
T3947	8007-8009	CD	denotes	40
T1954	1025-1234	sentence	denotes	Homozygous conditional knockout animals (Mtf1loxP/loxP) were crossed with the Cre recombinase transgenic line Mx-cre (a gift from Prof. Michel Aguet) to obtain an inducible, liver-specific Mtf1 knockout line.
T2075	1108-1119	NN	denotes	recombinase
T2076	1120-1130	JJ	denotes	transgenic
T2077	1136-1138	NN	denotes	Mx
T2078	1139-1142	NN	denotes	cre
T2079	1138-1139	HYPH	denotes	-
T2080	1143-1144	-LRB-	denotes	(
T2081	1146-1150	NN	denotes	gift
T2082	1144-1145	DT	denotes	a
T2083	1151-1155	IN	denotes	from
T2084	1156-1161	NNP	denotes	Prof.
T2085	1169-1174	NNP	denotes	Aguet
T2086	1162-1168	NNP	denotes	Michel
T2087	1174-1175	-RRB-	denotes	)
T2088	1176-1178	TO	denotes	to
T2089	1179-1185	VB	denotes	obtain
T2090	1186-1188	DT	denotes	an
T2091	1229-1233	NN	denotes	line
T2092	1189-1198	JJ	denotes	inducible
T2093	1198-1200	,	denotes	,
T2094	1200-1205	NN	denotes	liver
T2095	1206-1214	JJ	denotes	specific
T2096	1205-1206	HYPH	denotes	-
T2097	1215-1219	NN	denotes	Mtf1
T2098	1220-1228	NN	denotes	knockout
T2074	1104-1107	NN	denotes	Cre
R1032	T1759	T1758	prep	of,Generation
R1033	T1760	T1761	nmod	Mtf1,mice
R1034	T1761	T1759	pobj	mice,of
R1035	T1762	T1763	amod	conditional,knockout
R1036	T1763	T1761	compound	knockout,mice
R1037	T1764	T1761	cc	and,mice
R1038	T1765	T1766	npadvmod	liver,specific
R1039	T1766	T1768	amod	specific,deletion
R1040	T1767	T1766	punct	-,specific
R1041	T1768	T1761	conj	deletion,mice
R1042	T1770	T1771	nmod	Mtf1,mice
R1043	T1771	T1774	nsubjpass	mice,generated
R1044	T1772	T1773	amod	conditional,knockout
R1045	T1773	T1771	compound	knockout,mice
R1046	T1775	T1774	auxpass	were,generated
R1047	T1776	T1774	prep	in,generated
R1048	T1777	T1776	pobj	collaboration,in
R1049	T1778	T1777	prep	with,collaboration
R1050	T1779	T1780	compound	Dr,Leviten
R1051	T1780	T1778	pobj	Leviten,with
R1052	T1781	T1780	compound	Michael,Leviten
R1053	T1782	T1783	punct	(,Carlos
R1054	T1783	T1780	parataxis	Carlos,Leviten
R1055	T1784	T1783	compound	San,Carlos
R1056	T1785	T1783	punct	", ",Carlos
R1057	T1786	T1783	npadvmod	CA,Carlos
R1058	T1787	T1783	punct	),Carlos
R1059	T1788	T1774	punct	.,generated
R1060	T1790	T1791	nummod	Two,clones
R1061	T1791	T1793	nsubjpass	clones,used
R1062	T1792	T1791	amod	genomic,clones
R1063	T1794	T1791	acl	containing,clones
R1064	T1795	T1796	nmod	exons,3
R1065	T1796	T1794	dobj	3,containing
R1066	T1797	T1796	prep	to,3
R1067	T1798	T1797	pobj	6,to
R1068	T1799	T1796	prep	of,3
R1069	T1800	T1799	pobj	Mtf1,of
R1070	T1801	T1793	auxpass	were,used
R1071	T1802	T1803	aux	to,construct
R1072	T1803	T1793	advcl	construct,used
R1073	T1804	T1805	det	a,vector
R1074	T1805	T1803	dobj	vector,construct
R1075	T1806	T1807	compound	gene,targeting
R1076	T1807	T1805	compound	targeting,vector
R1077	T1808	T1803	prep	for,construct
R1078	T1809	T1810	amod	homologous,recombination
R1079	T1810	T1808	pobj	recombination,for
R1080	T1811	T1812	punct	(,Data
R1081	T1812	T1803	parataxis	Data,construct
R1082	T1813	T1812	amod	Supplementary,Data
R1083	T1814	T1812	punct	),Data
R1084	T1815	T1793	punct	.,used
R1085	T1817	T1818	det	A,cassette
R1086	T1818	T1821	nsubjpass	cassette,cloned
R1087	T1819	T1818	compound	neomycin,cassette
R1088	T1820	T1818	compound	resistance,cassette
R1089	T1821	T1833	ccomp	cloned,cloned
R1090	T1822	T1818	punct	(,cassette
R1091	T1823	T1824	compound	PGK,neo
R1092	T1824	T1818	appos	neo,cassette
R1093	T1825	T1824	punct	-,neo
R1094	T1826	T1818	punct	),cassette
R1095	T1827	T1818	acl	flanked,cassette
R1096	T1828	T1827	agent	by,flanked
R1097	T1829	T1830	nummod	two,sites
R1098	T1830	T1828	pobj	sites,by
R1099	T1831	T1830	compound	loxP,sites
R1100	T1832	T1821	auxpass	was,cloned
R1101	T1834	T1821	prep	into,cloned
R1102	T1835	T1836	det	the,site
R1103	T1836	T1834	pobj	site,into
R1104	T1837	T1836	compound	SacI,site
R1105	T1838	T1836	npadvmod	5,site
R1106	T1839	T1838	punct	′,5
R1107	T1840	T1838	prep	of,5
R1108	T1841	T1840	pobj	exon,of
R1109	T1842	T1841	nummod	3,exon
R1110	T1843	T1841	prep	of,exon
R1111	T1844	T1843	pobj	Mtf1,of
R1112	T1845	T1833	punct	", ",cloned
R1113	T1846	T1847	det	the,site
R1114	T1847	T1833	nsubjpass	site,cloned
R1115	T1848	T1847	amod	third,site
R1116	T1849	T1847	compound	loxP,site
R1117	T1850	T1833	auxpass	was,cloned
R1118	T1851	T1833	prep	into,cloned
R1119	T1852	T1853	det	the,site
R1120	T1853	T1851	pobj	site,into
R1121	T1854	T1853	compound	ScaI,site
R1122	T1855	T1853	npadvmod	3,site
R1123	T1856	T1855	punct	′,3
R1124	T1857	T1855	prep	of,3
R1125	T1858	T1857	pobj	exon,of
R1126	T1859	T1858	nummod	4,exon
R1127	T1860	T1833	punct	.,cloned
R1128	T1862	T1863	det	A,cassette
R1129	T1863	T1869	nsubjpass	cassette,inserted
R1130	T1864	T1865	nmod	thymidine,kinase
R1131	T1865	T1863	nmod	kinase,cassette
R1132	T1866	T1865	punct	(,kinase
R1133	T1867	T1865	appos	TK,kinase
R1134	T1868	T1863	punct	),cassette
R1135	T1870	T1869	auxpass	was,inserted
R1136	T1871	T1869	prep	in,inserted
R1137	T1872	T1873	det	the,site
R1138	T1873	T1871	pobj	site,in
R1139	T1874	T1873	compound	HpaI,site
R1140	T1875	T1873	npadvmod	3,site
R1141	T1876	T1875	punct	′,3
R1142	T1877	T1875	prep	of,3
R1143	T1878	T1877	pobj	exon,of
R1144	T1879	T1878	nummod	6,exon
R1145	T1880	T1869	punct	.,inserted
R1146	T1882	T1883	nummod	129,cells
R1147	T1883	T1885	nsubjpass	cells,electroporated
R1148	T1884	T1883	compound	ES,cells
R1149	T1886	T1885	auxpass	were,electroporated
R1150	T1887	T1885	prep	with,electroporated
R1151	T1888	T1889	det	the,vector
R1152	T1889	T1887	pobj	vector,with
R1153	T1890	T1889	amod	linearized,vector
R1154	T1891	T1889	compound	targeting,vector
R1155	T1892	T1889	punct	", ",vector
R1156	T1893	T1889	acl	selected,vector
R1157	T1894	T1893	prep	in,selected
R1158	T1895	T1896	det	the,presence
R1159	T1896	T1894	pobj	presence,in
R1160	T1897	T1896	prep	of,presence
R1161	T1898	T1897	pobj	G418,of
R1162	T1899	T1898	cc	and,G418
R1163	T1900	T1898	conj	FIAU,G418
R1164	T1901	T1885	punct	", ",electroporated
R1165	T1902	T1885	cc	and,electroporated
R1166	T1903	T1885	conj	screened,electroporated
R1167	T1904	T1903	prep	for,screened
R1168	T1905	T1906	amod	correct,events
R1169	T1906	T1904	pobj	events,for
R1170	T1907	T1906	compound	integration,events
R1171	T1908	T1903	prep	by,screened
R1172	T1909	T1910	nmod	PCR,analysis
R1173	T1910	T1908	pobj	analysis,by
R1174	T1911	T1909	cc	and,PCR
R1175	T1912	T1913	compound	Southern,blot
R1176	T1913	T1909	conj	blot,PCR
R1177	T1914	T1885	punct	.,electroporated
R1178	T1916	T1917	amod	Transient,expression
R1179	T1917	T1918	nsubj	expression,led
R1180	T1919	T1917	prep	of,expression
R1181	T1920	T1921	compound	Cre,recombinase
R1182	T1921	T1919	pobj	recombinase,of
R1183	T1922	T1918	prep	to,led
R1184	T1923	T1922	pobj	removal,to
R1185	T1924	T1923	prep	of,removal
R1186	T1925	T1926	det	the,cassette
R1187	T1926	T1924	pobj	cassette,of
R1188	T1927	T1928	compound	PGK,neo
R1189	T1928	T1926	compound	neo,cassette
R1190	T1929	T1928	punct	-,neo
R1191	T1930	T1918	punct	", ",led
R1192	T1931	T1918	cc	and,led
R1193	T1932	T1933	nsubjpass	mice,generated
R1194	T1933	T1918	conj	generated,led
R1195	T1934	T1932	acl	carrying,mice
R1196	T1935	T1936	det	the,allele
R1197	T1936	T1934	dobj	allele,carrying
R1198	T1937	T1936	amod	modified,allele
R1199	T1938	T1936	compound	Mtf1loxP,allele
R1200	T1939	T1933	auxpass	were,generated
R1201	T1940	T1933	prep	by,generated
R1202	T1941	T1940	pobj	injection,by
R1203	T1942	T1941	prep	of,injection
R1204	T1943	T1944	amod	positive,clones
R1205	T1944	T1942	pobj	clones,of
R1206	T1945	T1941	prep	into,injection
R1207	T1946	T1947	nmod	C57Bl,blastocysts
R1208	T1947	T1945	pobj	blastocysts,into
R1209	T1948	T1946	punct	/,C57Bl
R1210	T1949	T1946	nummod	6,C57Bl
R1211	T1950	T1947	cc	and,blastocysts
R1212	T1951	T1952	amod	subsequent,crosses
R1213	T1952	T1947	conj	crosses,blastocysts
R1214	T1953	T1933	punct	.,generated
R1215	T1955	T1956	amod	Homozygous,animals
R1216	T1956	T1959	nsubjpass	animals,crossed
R1217	T1957	T1958	amod	conditional,knockout
R1218	T1958	T1956	compound	knockout,animals
R1219	T1960	T1956	punct	(,animals
R1220	T1961	T1962	compound	Mtf1loxP,loxP
R1221	T1962	T1956	appos	loxP,animals
R1222	T1963	T1962	punct	/,loxP
R1223	T1964	T1959	punct	),crossed
R1224	T1965	T1959	auxpass	were,crossed
R1225	T1966	T1959	prep	with,crossed
R1226	T1967	T1968	det	the,line
R1227	T1968	T1966	pobj	line,with
R1228	T2074	T2075	compound	Cre,recombinase
R1229	T2075	T2076	npadvmod	recombinase,transgenic
R1230	T2076	T1968	amod	transgenic,line
R1231	T2077	T2078	compound	Mx,cre
R1232	T2078	T1968	appos	cre,line
R1233	T2079	T2078	punct	-,cre
R1234	T2080	T2081	punct	(,gift
R1235	T2081	T1968	parataxis	gift,line
R1236	T2082	T2081	det	a,gift
R1237	T2083	T2081	prep	from,gift
R1238	T2084	T2085	compound	Prof.,Aguet
R1239	T2085	T2083	pobj	Aguet,from
R1240	T2086	T2085	compound	Michel,Aguet
R1241	T2087	T2081	punct	),gift
R1242	T2088	T2089	aux	to,obtain
R1243	T2089	T1959	advcl	obtain,crossed
R1244	T2090	T2091	det	an,line
R1245	T2091	T2089	dobj	line,obtain
R1246	T2092	T2091	amod	inducible,line
R1247	T2093	T2091	punct	", ",line
R1248	T2094	T2095	npadvmod	liver,specific
R1249	T2095	T2091	amod	specific,line
R1250	T2096	T2095	punct	-,specific
R1251	T2097	T2098	compound	Mtf1,knockout
R1252	T2098	T2091	compound	knockout,line
R1253	T2099	T1959	punct	.,crossed
R1254	T2101	T2102	det	The,mice
R1255	T2102	T2103	nsubjpass	mice,genotyped
R1256	T2104	T2103	auxpass	were,genotyped
R1257	T2105	T2103	prep	by,genotyped
R1258	T2106	T2105	pobj	PCR,by
R1259	T2107	T2103	advcl	using,genotyped
R1260	T2108	T2109	det	the,primers
R1261	T2109	T2107	dobj	primers,using
R1262	T2110	T2109	amod	following,primers
R1263	T2111	T2112	punct	(,Microsynth
R1264	T2112	T2109	parataxis	Microsynth,primers
R1265	T2113	T2112	punct	),Microsynth
R1266	T2114	T2103	punct	: ,genotyped
R1267	T2115	T2116	compound	Cre,recombinase
R1268	T2116	T2117	dep	recombinase,CCAGGTTACGGATATAGTTCATGAC
R1269	T2117	T2130	dep	CCAGGTTACGGATATAGTTCATGAC,CAGTCACAAGCAAATTACCAAACACTGCC
R1270	T2118	T2117	punct	: ,CCAGGTTACGGATATAGTTCATGAC
R1271	T2119	T2120	nummod	5,CTATCCAGCAACATTTGGGCCAGC
R1272	T2120	T2117	dep	CTATCCAGCAACATTTGGGCCAGC,CCAGGTTACGGATATAGTTCATGAC
R1273	T2121	T2119	punct	′,5
R1274	T2122	T2120	punct	-,CTATCCAGCAACATTTGGGCCAGC
R1275	T2123	T2120	punct	-,CTATCCAGCAACATTTGGGCCAGC
R1276	T2124	T2120	nummod	3,CTATCCAGCAACATTTGGGCCAGC
R1277	T2125	T2120	punct	′,CTATCCAGCAACATTTGGGCCAGC
R1278	T2126	T2117	punct	;,CCAGGTTACGGATATAGTTCATGAC
R1279	T2127	T2117	nummod	5,CCAGGTTACGGATATAGTTCATGAC
R1280	T2128	T2127	punct	′,5
R1281	T2129	T2117	punct	-,CCAGGTTACGGATATAGTTCATGAC
R1282	T2130	T2103	dep	CAGTCACAAGCAAATTACCAAACACTGCC,genotyped
R1283	T2131	T2117	punct	-,CCAGGTTACGGATATAGTTCATGAC
R1284	T2132	T2117	nummod	3,CCAGGTTACGGATATAGTTCATGAC
R1285	T2133	T2117	punct	′,CCAGGTTACGGATATAGTTCATGAC
R1286	T2134	T2130	punct	", ",CAGTCACAAGCAAATTACCAAACACTGCC
R1287	T2135	T2130	dep	Mtf1loxP,CAGTCACAAGCAAATTACCAAACACTGCC
R1288	T2136	T2135	cc	or,Mtf1loxP
R1289	T2137	T2138	amod	wild,type
R1290	T2138	T2140	compound	type,allele
R1291	T2139	T2138	punct	-,type
R1292	T2140	T2135	conj	allele,Mtf1loxP
R1293	T2141	T2130	punct	: ,CAGTCACAAGCAAATTACCAAACACTGCC
R1294	T2142	T2143	nummod	5,CACACCCAGTTTGTGTATGTCTTC
R1295	T2143	T2130	dep	CACACCCAGTTTGTGTATGTCTTC,CAGTCACAAGCAAATTACCAAACACTGCC
R1296	T2144	T2142	punct	′,5
R1297	T2145	T2143	punct	-,CACACCCAGTTTGTGTATGTCTTC
R1298	T2146	T2143	punct	-,CACACCCAGTTTGTGTATGTCTTC
R1299	T2147	T2143	nummod	3,CACACCCAGTTTGTGTATGTCTTC
R1300	T2148	T2143	punct	′,CACACCCAGTTTGTGTATGTCTTC
R1301	T2149	T2130	punct	;,CAGTCACAAGCAAATTACCAAACACTGCC
R1302	T2150	T2130	nummod	5,CAGTCACAAGCAAATTACCAAACACTGCC
R1303	T2151	T2150	punct	′,5
R1304	T2152	T2130	punct	-,CAGTCACAAGCAAATTACCAAACACTGCC
R1305	T2153	T2130	punct	-,CAGTCACAAGCAAATTACCAAACACTGCC
R1306	T2154	T2130	nummod	3,CAGTCACAAGCAAATTACCAAACACTGCC
R1307	T2155	T2130	punct	′,CAGTCACAAGCAAATTACCAAACACTGCC
R1308	T2156	T2103	punct	.,genotyped
R1309	T2173	T2174	compound	Animal,treatment
R1310	T2176	T2177	prep	At,received
R1311	T2178	T2179	nummod	8,weeks
R1312	T2179	T2176	pobj	weeks,At
R1313	T2180	T2179	prep	of,weeks
R1314	T2181	T2180	pobj	age,of
R1315	T2182	T2177	punct	", ",received
R1316	T2183	T2184	amod	male,mice
R1317	T2184	T2177	nsubj	mice,received
R1318	T2185	T2186	compound	Mtf1loxP,loxP
R1319	T2186	T2184	compound	loxP,mice
R1320	T2187	T2186	punct	/,loxP
R1321	T2188	T2184	acl	harboring,mice
R1322	T2189	T2190	det	the,transgene
R1323	T2190	T2188	dobj	transgene,harboring
R1324	T2191	T2192	compound	Mx,cre
R1325	T2192	T2190	compound	cre,transgene
R1326	T2193	T2192	punct	-,cre
R1327	T2194	T2190	punct	(,transgene
R1328	T2195	T2196	compound	Mtf1loxP,cre
R1330	T2197	T2196	punct	/,cre
R1331	T2198	T2196	compound	loxP,cre
R1332	T2199	T2196	compound	Mx,cre
R1333	T2200	T2196	punct	-,cre
R1334	T2201	T2196	punct	", ",cre
R1335	T2202	T2203	advmod	abbr.,cre
R1336	T2203	T2196	appos	cre,cre
R1337	T2204	T2203	punct	: ,cre
R1338	T2205	T2203	compound	Mtf1Mx,cre
R1339	T2206	T2203	punct	-,cre
R1340	T2207	T2190	punct	),transgene
R1341	T2208	T2190	cc	and,transgene
R1342	T2209	T2210	compound	control,littermates
R1343	T2210	T2190	conj	littermates,transgene
R1344	T2211	T2210	prep	without,littermates
R1345	T2212	T2211	pobj	transgene,without
R1346	T2213	T2210	punct	(,littermates
R1347	T2214	T2215	compound	Mtf1loxP,loxP
R1348	T2215	T2210	appos	loxP,littermates
R1349	T2216	T2215	punct	/,loxP
R1350	T2217	T2215	punct	", ",loxP
R1351	T2218	T2219	advmod	abbr.,Mtf1loxP
R1352	T2219	T2215	appos	Mtf1loxP,loxP
R1353	T2220	T2219	punct	: ,Mtf1loxP
R1354	T2221	T2177	punct	),received
R1355	T2222	T2223	nummod	four,injections
R1356	T2223	T2177	dobj	injections,received
R1357	T2224	T2223	amod	intraperitoneal,injections
R1358	T2225	T2223	advmod	each,injections
R1359	T2226	T2223	prep	of,injections
R1360	T2227	T2228	nummod	300,µg
R1361	T2228	T2229	nmod	µg,acid
R1362	T2229	T2226	pobj	acid,of
R1363	T2230	T2229	amod	synthetic,acid
R1364	T2231	T2232	amod	double,stranded
R1365	T2232	T2229	amod	stranded,acid
R1366	T2233	T2323	nummod	20,µmol
R1367	T2234	T2232	punct	-,stranded
R1368	T2235	T2229	nmod	RNA,acid
R1369	T2236	T2237	amod	polyinosinic,polycytidylic
R1370	T2237	T2229	amod	polycytidylic,acid
R1371	T2238	T2237	punct	–,polycytidylic
R1372	T2239	T2240	punct	[,Sigma
R1373	T2240	T2229	parataxis	Sigma,acid
R1374	T2241	T2242	compound	pI,pC
R1375	T2242	T2240	dep	pC,Sigma
R1376	T2243	T2242	punct	–,pC
R1377	T2244	T2240	punct	;,Sigma
R1378	T2245	T2240	punct	;,Sigma
R1379	T2246	T2240	prep	in,Sigma
R1380	T2247	T2248	det	a,volume
R1381	T2248	T2246	pobj	volume,in
R1382	T2249	T2248	prep	of,volume
R1383	T2250	T2251	nummod	60,µl
R1384	T2251	T2249	pobj	µl,of
R1385	T2252	T2253	npadvmod	phosphate,buffered
R1386	T2253	T2255	amod	buffered,saline
R1387	T2254	T2253	punct	-,buffered
R1388	T2255	T2251	appos	saline,µl
R1389	T2256	T2255	punct	(,saline
R1390	T2257	T2255	appos	PBS,saline
R1391	T2258	T2240	punct	),Sigma
R1392	T2259	T2240	punct	],Sigma
R1393	T2260	T2177	prep	at,received
R1394	T2261	T2262	nummod	3,day
R1395	T2262	T2263	compound	day,intervals
R1396	T2263	T2260	pobj	intervals,at
R1397	T2264	T2177	punct	.,received
R1398	T2266	T2267	advmod	Only,in
R1399	T2267	T2268	prep	in,received
R1400	T2269	T2270	det	the,case
R1401	T2270	T2267	pobj	case,in
R1402	T2271	T2270	prep	of,case
R1403	T2272	T2273	npadvmod	DNA,binding
R1404	T2273	T2275	amod	binding,studies
R1405	T2274	T2273	punct	-,binding
R1406	T2275	T2271	pobj	studies,of
R1407	T2276	T2275	prep	with,studies
R1408	T2277	T2278	compound	MRE,sequences
R1409	T2278	T2276	pobj	sequences,with
R1410	T2279	T2278	prep	from,sequences
R1411	T2280	T2281	nmod	MTF,candidates
R1412	T2281	T2279	pobj	candidates,from
R1413	T2282	T2280	punct	-,MTF
R1414	T2283	T2280	nummod	1,MTF
R1415	T2284	T2285	compound	target,gene
R1416	T2285	T2281	compound	gene,candidates
R1417	T2286	T2268	punct	", ",received
R1418	T2287	T2288	det	the,mice
R1419	T2288	T2268	nsubj	mice,received
R1420	T2289	T2288	compound	control,mice
R1421	T2290	T2291	det	no,injections
R1422	T2291	T2268	dobj	injections,received
R1423	T2292	T2293	compound	pI,pC
R1424	T2293	T2291	compound	pC,injections
R1425	T2294	T2293	punct	–,pC
R1426	T2295	T2268	punct	.,received
R1427	T2297	T2298	prep	For,received
R1428	T2299	T2297	pobj	experiments,For
R1429	T2300	T2299	prep	with,experiments
R1430	T2301	T2302	compound	metal,treatment
R1431	T2302	T2300	pobj	treatment,with
R1432	T2303	T2298	punct	", ",received
R1433	T2304	T2298	nsubj	mice,received
R1434	T2305	T2306	nummod	2,days
R1435	T2306	T2307	npadvmod	days,after
R1436	T2307	T2298	prep	after,received
R1437	T2308	T2309	det	the,treatment
R1438	T2309	T2307	pobj	treatment,after
R1439	T2310	T2309	amod	last,treatment
R1440	T2311	T2312	compound	pI,pC
R1441	T2312	T2309	compound	pC,treatment
R1442	T2313	T2312	punct	–,pC
R1443	T2314	T2315	det	a,injection
R1444	T2315	T2298	dobj	injection,received
R1445	T2316	T2317	amod	subcutaneous,s.c.
R1446	T2317	T2315	amod	s.c.,injection
R1447	T2318	T2317	punct	(,s.c.
R1448	T2319	T2315	punct	),injection
R1449	T2320	T2315	prep	of,injection
R1450	T2321	T2322	preconj	either,CdSO4
R1451	T2322	T2320	pobj	CdSO4,of
R1452	T2323	T2322	nmod	µmol,CdSO4
R1453	T2324	T2325	punct	/,weight
R1454	T2325	T2323	prep	weight,µmol
R1455	T2326	T2325	compound	kg,weight
R1456	T2327	T2325	compound	body,weight
R1457	T2328	T2329	punct	(,treatment
R1458	T2329	T2322	parataxis	treatment,CdSO4
R1459	T2330	T2331	nummod	2,mM
R1460	T2331	T2332	compound	mM,CdSO4
R1461	T2332	T2329	dep	CdSO4,treatment
R1462	T2333	T2332	prep	in,CdSO4
R1463	T2334	T2333	pobj	H2O,in
R1464	T2335	T2329	punct	;,treatment
R1465	T2336	T2329	compound	cadmium,treatment
R1466	T2337	T2329	punct	),treatment
R1467	T2338	T2322	cc	or,CdSO4
R1468	T2339	T2340	nummod	10,ml
R1469	T2340	T2341	nmod	ml,H2O
R1471	T2342	T2343	punct	/,weight
R1472	T2343	T2340	prep	weight,ml
R1473	T2344	T2343	compound	kg,weight
R1474	T2345	T2343	compound	body,weight
R1475	T2346	T2347	punct	(,treatment
R1476	T2347	T2341	parataxis	treatment,H2O
R1477	T2348	T2347	amod	mock,treatment
R1478	T2349	T2347	punct	),treatment
R1479	T2350	T2351	nummod	6,h
R1480	T2351	T2352	npadvmod	h,before
R1481	T2352	T2298	prep	before,received
R1482	T2353	T2352	pcomp	sacrificing,before
R1483	T2354	T2353	dobj	them,sacrificing
R1484	T2355	T2298	punct	.,received
R1487	T2531	T2532	compound	Microarray,analysis
R1488	T2533	T2532	cc	and,analysis
R1489	T2534	T2535	compound	data,processing
R1490	T2535	T2532	conj	processing,analysis
R1491	T2537	T2538	amod	Total,RNA
R1492	T2538	T2539	nsubjpass	RNA,isolated
R1493	T2540	T2539	auxpass	was,isolated
R1494	T2541	T2539	prep	from,isolated
R1495	T2542	T2543	compound	liver,tissue
R1496	T2543	T2541	pobj	tissue,from
R1497	T2544	T2543	prep	of,tissue
R1498	T2545	T2546	compound	pI,pC
R1499	T2546	T2548	npadvmod	pC,induced
R1500	T2547	T2546	punct	–,pC
R1501	T2548	T2550	amod	induced,mice
R1502	T2549	T2548	punct	-,induced
R1503	T2550	T2544	pobj	mice,of
R1504	T2551	T2550	punct	", ",mice
R1505	T2552	T2553	amod	mock,treated
R1506	T2553	T2550	amod	treated,mice
R1507	T2554	T2552	punct	-,mock
R1508	T2555	T2552	cc	or,mock
R1509	T2556	T2552	conj	cadmium,mock
R1510	T2557	T2553	punct	-,treated
R1511	T2558	T2559	nmod	Mtf1Mx,cre
R1512	T2559	T2550	nmod	cre,mice
R1513	T2560	T2559	punct	-,cre
R1514	T2561	T2559	cc	and,cre
R1515	T2562	T2559	conj	Mtf1loxP,cre
R1516	T2563	T2564	punct	(,male
R1517	T2564	T2544	parataxis	male,of
R1518	T2565	T2566	nsubj	n,3
R1519	T2566	T2564	ccomp	3,male
R1520	T2567	T2566	punct	=,3
R1521	T2568	T2566	prep	per,3
R1522	T2569	T2568	pobj	genotype,per
R1523	T2570	T2569	cc	and,genotype
R1524	T2571	T2572	amod	respective,treatment
R1525	T2572	T2569	conj	treatment,genotype
R1526	T2573	T2564	punct	;,male
R1527	T2574	T2564	det	all,male
R1528	T2575	T2564	punct	),male
R1529	T2576	T2577	advmod	essentially,described
R1530	T2577	T2539	advcl	described,isolated
R1531	T2578	T2577	mark	as,described
R1532	T2579	T2577	agent	by,described
R1533	T2580	T2579	pobj	Chomczynski,by
R1534	T2581	T2580	cc	and,Chomczynski
R1535	T2582	T2580	conj	Sacchi,Chomczynski
R1536	T2583	T2584	punct	(,27
R1537	T2584	T2577	parataxis	27,described
R1538	T2585	T2584	punct	),27
R1539	T2586	T2539	punct	.,isolated
R1540	T2588	T2589	compound	Gene,expression
R1541	T2589	T2590	compound	expression,analysis
R1542	T2590	T2591	nsubjpass	analysis,performed
R1543	T2592	T2591	auxpass	was,performed
R1544	T2593	T2591	prep	in,performed
R1545	T2594	T2595	det	the,Center
R1546	T2595	T2593	pobj	Center,in
R1547	T2596	T2597	compound	Functional,Genomics
R1548	T2597	T2595	compound	Genomics,Center
R1549	T2598	T2595	npadvmod	Zurich,Center
R1550	T2599	T2591	advcl	using,performed
R1551	T2600	T2601	nmod	GeneChip®,Arrays
R1552	T2601	T2599	dobj	Arrays,using
R1553	T2602	T2603	nmod	Mouse,Genome
R1554	T2603	T2601	nmod	Genome,Arrays
R1555	T2604	T2603	nummod	430,Genome
R1556	T2605	T2601	nummod	2.0,Arrays
R1557	T2606	T2607	punct	(,Affymetrix
R1558	T2607	T2601	parataxis	Affymetrix,Arrays
R1559	T2608	T2607	punct	),Affymetrix
R1560	T2609	T2599	prep	according,using
R1561	T2610	T2609	prep	to,according
R1562	T2611	T2612	det	the,manufacturer
R1563	T2612	T2613	poss	manufacturer,instructions
R1564	T2613	T2610	pobj	instructions,to
R1565	T2614	T2612	case	's,manufacturer
R1566	T2615	T2613	cc	and,instructions
R1567	T2616	T2617	det	the,reagents
R1568	T2617	T2613	conj	reagents,instructions
R1569	T2618	T2617	amod	following,reagents
R1570	T2619	T2617	cc	and,reagents
R1571	T2620	T2617	conj	conditions,reagents
R1572	T2621	T2591	punct	.,performed
R1573	T2623	T2624	nsubjpass	cDNA,synthesized
R1574	T2625	T2624	auxpass	was,synthesized
R1575	T2626	T2624	prep	with,synthesized
R1576	T2627	T2628	nmod	SuperScript,Kit
R1578	T2629	T2627	punct	™,SuperScript
R1579	T2630	T2631	amod	Double,Stranded
R1580	T2631	T2628	amod	Stranded,Kit
R1581	T2632	T2631	punct	-,Stranded
R1582	T2633	T2628	compound	cDNA,Kit
R1583	T2634	T2628	compound	Synthesis,Kit
R1584	T2635	T2636	punct	(,Invitrogen
R1585	T2636	T2628	parataxis	Invitrogen,Kit
R1586	T2637	T2636	punct	),Invitrogen
R1587	T2638	T2624	punct	", ",synthesized
R1588	T2639	T2624	advcl	using,synthesized
R1589	T2640	T2641	nummod	15,µg
R1590	T2641	T2642	nmod	µg,RNA
R1591	T2642	T2639	dobj	RNA,using
R1592	T2643	T2642	amod	total,RNA
R1593	T2644	T2624	punct	.,synthesized
R1594	T2646	T2647	advmod	In,vitro
R1595	T2647	T2648	amod	vitro,transcription
R1596	T2648	T2649	nsubjpass	transcription,performed
R1597	T2650	T2649	auxpass	was,performed
R1598	T2651	T2649	prep	with,performed
R1599	T2652	T2653	nmod	BioArray,Kit
R1600	T2653	T2651	pobj	Kit,with
R1601	T2654	T2652	punct	™,BioArray
R1602	T2655	T2656	nmod	High,Yield
R1603	T2656	T2653	nmod	Yield,Kit
R1604	T2657	T2656	punct	™,Yield
R1605	T2658	T2653	compound	RNA,Kit
R1606	T2659	T2653	compound	Transcript,Kit
R1607	T2660	T2653	compound	Labeling,Kit
R1608	T2661	T2662	punct	(,Enzo
R1609	T2662	T2653	parataxis	Enzo,Kit
R1610	T2663	T2662	punct	),Enzo
R1611	T2664	T2653	cc	and,Kit
R1612	T2665	T2666	quantmod	3.5,6
R1613	T2666	T2668	nummod	6,µg
R1614	T2667	T2666	quantmod	to,6
R1615	T2668	T2653	conj	µg,Kit
R1616	T2669	T2668	prep	of,µg
R1617	T2670	T2671	det	each,cDNA
R1618	T2671	T2669	pobj	cDNA,of
R1619	T2672	T2649	punct	.,performed
R1620	T2674	T2675	compound	Clean,up
R1621	T2675	T2677	nsubjpass	up,done
R1622	T2676	T2675	punct	-,up
R1623	T2678	T2675	prep	of,up
R1624	T2679	T2680	preconj	both,cDNA
R1625	T2680	T2681	nmod	cDNA,samples
R1626	T2681	T2678	pobj	samples,of
R1627	T2682	T2680	cc	and,cDNA
R1628	T2683	T2680	conj	cRNA,cDNA
R1629	T2684	T2677	auxpass	was,done
R1630	T2685	T2677	advcl	using,done
R1631	T2686	T2687	compound	GeneChip®,Module
R1632	T2687	T2685	dobj	Module,using
R1633	T2688	T2687	compound	Sample,Module
R1634	T2689	T2690	compound	Clean,up
R1635	T2690	T2687	compound	up,Module
R1636	T2691	T2690	punct	-,up
R1637	T2692	T2693	punct	(,Affymetrix
R1638	T2693	T2687	parataxis	Affymetrix,Module
R1639	T2694	T2693	punct	),Affymetrix
R1640	T2695	T2677	punct	.,done
R1641	T2697	T2698	prep	For,used
R1642	T2699	T2700	det	the,washing
R1643	T2700	T2697	pobj	washing,For
R1644	T2701	T2700	amod	automated,washing
R1645	T2702	T2700	cc	and,washing
R1646	T2703	T2700	conj	staining,washing
R1647	T2704	T2700	prep	in,washing
R1648	T2705	T2706	det	the,station
R1649	T2706	T2704	pobj	station,in
R1650	T2707	T2706	compound	Affymetrix,station
R1651	T2708	T2706	compound	fluidics,station
R1652	T2709	T2706	nummod	450,station
R1653	T2710	T2698	punct	", ",used
R1654	T2711	T2712	det	the,Ws2v4_450
R1655	T2712	T2698	nsubjpass	Ws2v4_450,used
R1656	T2713	T2712	compound	protocol,Ws2v4_450
R1657	T2714	T2712	compound	EukGE,Ws2v4_450
R1658	T2715	T2712	punct	-,Ws2v4_450
R1659	T2716	T2698	auxpass	was,used
R1660	T2717	T2698	punct	.,used
R1661	T2719	T2720	det	The,arrays
R1662	T2720	T2722	nsubjpass	arrays,scanned
R1663	T2721	T2720	compound	probe,arrays
R1664	T2723	T2722	auxpass	were,scanned
R1665	T2724	T2722	prep	with,scanned
R1666	T2725	T2726	det	the,scanner
R1667	T2726	T2724	pobj	scanner,with
R1668	T2727	T2728	nmod	Affymetrix,GS
R1669	T2728	T2726	nmod	GS,scanner
R1670	T2729	T2728	nummod	3000,GS
R1671	T2730	T2722	punct	.,scanned
R1672	T2732	T2733	amod	Raw,data
R1673	T2733	T2734	nsubj	data,are
R1674	T2735	T2734	acomp	available,are
R1675	T2736	T2734	prep	at,are
R1676	T2737	T2736	pobj	ArrayExpress,at
R1677	T2738	T2739	punct	(,MEXP
R1678	T2739	T2734	parataxis	MEXP,are
R1679	T2740	T2739	punct	", ",MEXP
R1680	T2741	T2742	compound	accession,number
R1681	T2742	T2739	compound	number,MEXP
R1682	T2743	T2739	compound	E,MEXP
R1683	T2744	T2739	punct	-,MEXP
R1684	T2745	T2739	punct	-,MEXP
R1685	T2746	T2739	nummod	438,MEXP
R1686	T2747	T2739	punct	),MEXP
R1687	T2748	T2734	punct	.,are
R1688	T2750	T2751	compound	Data,analysis
R1689	T2751	T2752	nsubjpass	analysis,performed
R1690	T2753	T2752	auxpass	was,performed
R1691	T2754	T2752	prep	with,performed
R1692	T2755	T2756	nmod	GeneSpring,software
R1693	T2756	T2754	pobj	software,with
R1694	T2757	T2756	nummod	6.1,software
R1695	T2758	T2759	punct	(,Genetics
R1696	T2759	T2756	parataxis	Genetics,software
R1697	T2760	T2759	compound	Silicon,Genetics
R1698	T2761	T2759	punct	),Genetics
R1699	T2762	T2752	punct	", ",performed
R1700	T2763	T2752	advcl	applying,performed
R1701	T2764	T2765	det	a,level
R1702	T2765	T2763	dobj	level,applying
R1703	T2766	T2765	compound	significance,level
R1704	T2767	T2768	nsubj	P,0.05
R1705	T2768	T2765	appos	0.05,level
R1706	T2769	T2768	punct	≤,0.05
R1707	T2770	T2752	punct	.,performed
R1708	T2772	T2773	advmod	Furthermore,used
R1709	T2774	T2773	punct	", ",used
R1710	T2775	T2776	amod	multiple,correction
R1711	T2776	T2773	nsubjpass	correction,used
R1712	T2777	T2776	compound	testing,correction
R1713	T2778	T2773	auxpass	was,used
R1714	T2779	T2773	prep	in,used
R1715	T2780	T2779	pobj	addition,in
R1716	T2781	T2782	aux	to,obtain
R1717	T2782	T2773	advcl	obtain,used
R1718	T2783	T2784	npadvmod	cadmium,responsive
R1719	T2784	T2786	amod	responsive,genes
R1720	T2785	T2784	punct	-,responsive
R1722	T2787	T2786	punct	", ",genes
R1723	T2788	T2789	npadvmod	MTF,independent
R1724	T2789	T2786	amod	independent,genes
R1725	T2790	T2788	punct	-,MTF
R1726	T2791	T2788	nummod	1,MTF
R1727	T2792	T2789	punct	-,independent
R1728	T2793	T2773	punct	.,used
R1729	T2795	T2796	nsubjpass	Genes,considered
R1730	T2797	T2796	auxpass	were,considered
R1731	T2798	T2799	aux	to,expressed
R1732	T2799	T2796	xcomp	expressed,considered
R1733	T2800	T2799	auxpass	be,expressed
R1734	T2801	T2799	advmod	differentially,expressed
R1735	T2802	T2803	mark	if,was
R1736	T2803	T2799	advcl	was,expressed
R1737	T2804	T2803	expl	there,was
R1738	T2805	T2806	advmod	at,least
R1739	T2806	T2807	advmod	least,2-fold
R1740	T2807	T2809	nummod	2-fold,difference
R1741	T2808	T2807	quantmod	a,2-fold
R1742	T2809	T2803	attr	difference,was
R1743	T2810	T2809	prep	in,difference
R1744	T2811	T2812	compound	expression,levels
R1745	T2812	T2810	pobj	levels,in
R1746	T2813	T2812	prep	of,levels
R1747	T2814	T2815	det	the,groups
R1748	T2815	T2813	pobj	groups,of
R1749	T2816	T2815	amod	compared,groups
R1750	T2817	T2815	amod	experimental,groups
R1751	T2818	T2819	punct	(,genotype
R1752	T2819	T2803	parataxis	genotype,was
R1753	T2820	T2819	cc	and,genotype
R1754	T2821	T2822	amod	respective,treatment
R1755	T2822	T2819	conj	treatment,genotype
R1756	T2823	T2819	punct	),genotype
R1757	T2824	T2796	punct	.,considered
R1758	T2826	T2827	det	The,result
R1759	T2827	T2828	nsubjpass	result,considered
R1760	T2829	T2828	auxpass	was,considered
R1761	T2830	T2828	oprd	reliable,considered
R1762	T2831	T2832	mark	if,scored
R1763	T2832	T2828	advcl	scored,considered
R1764	T2833	T2834	compound	signal,values
R1765	T2834	T2832	nsubjpass	values,scored
R1766	T2835	T2834	prep	for,values
R1767	T2836	T2837	det	the,gene
R1768	T2837	T2835	pobj	gene,for
R1769	T2838	T2837	amod	respective,gene
R1770	T2839	T2832	auxpass	were,scored
R1771	T2840	T2841	punct	‘,present
R1772	T2841	T2832	oprd	present,scored
R1773	T2842	T2832	punct	’,scored
R1774	T2843	T2844	advmod	at,least
R1775	T2844	T2845	advmod	least,for
R1776	T2845	T2828	prep	for,considered
R1777	T2846	T2847	det	all,mice
R1778	T2847	T2845	pobj	mice,for
R1779	T2848	T2847	prep	in,mice
R1780	T2849	T2850	nummod	one,group
R1781	T2850	T2848	pobj	group,in
R1782	T2851	T2850	amod	experimental,group
R1783	T2852	T2845	cc	or,for
R1784	T2853	T2845	conj	for,for
R1785	T2854	T2855	nummod	two,mice
R1786	T2855	T2853	pobj	mice,for
R1787	T2856	T2855	prep	in,mice
R1788	T2857	T2856	pobj	each,in
R1789	T2858	T2857	prep	of,each
R1790	T2859	T2860	nummod	two,groups
R1791	T2860	T2858	pobj	groups,of
R1792	T2861	T2828	punct	.,considered
R1793	T2863	T2864	aux	To,screen
R1794	T2864	T2865	advcl	screen,obtained
R1795	T2866	T2864	prep	for,screen
R1796	T2867	T2868	det	the,presence
R1797	T2868	T2866	pobj	presence,for
R1798	T2869	T2868	prep	of,presence
R1799	T2870	T2871	det	the,sequence
R1800	T2871	T2869	pobj	sequence,of
R1801	T2872	T2871	compound	MRE,sequence
R1802	T2873	T2871	compound	core,sequence
R1803	T2874	T2871	compound	consensus,sequence
R1804	T2875	T2871	appos	TGCRCNC,sequence
R1805	T2876	T2868	prep	in,presence
R1806	T2877	T2878	det	the,region
R1807	T2878	T2876	pobj	region,in
R1808	T2879	T2878	compound	promoter,region
R1809	T2880	T2865	punct	", ",obtained
R1810	T2881	T2882	det	the,sequences
R1811	T2882	T2865	nsubjpass	sequences,obtained
R1812	T2883	T2882	amod	upstream,sequences
R1813	T2884	T2882	prep	of,sequences
R1814	T2885	T2886	det	the,genes
R1815	T2886	T2884	pobj	genes,of
R1816	T2887	T2886	amod	respective,genes
R1817	T2888	T2865	auxpass	were,obtained
R1818	T2889	T2865	prep	from,obtained
R1819	T2890	T2891	det	the,Database
R1820	T2891	T2889	pobj	Database,from
R1821	T2892	T2891	nmod	University,Database
R1822	T2893	T2892	prep	of,University
R1823	T2894	T2895	compound	California,Cruz
R1824	T2895	T2893	pobj	Cruz,of
R1825	T2896	T2895	compound	Santa,Cruz
R1826	T2897	T2892	punct	(,University
R1827	T2898	T2892	appos	UCSC,University
R1828	T2899	T2891	punct	),Database
R1829	T2900	T2891	compound	Genome,Database
R1830	T2901	T2891	compound	Browser,Database
R1831	T2902	T2903	punct	(,November
R1832	T2903	T2891	parataxis	November,Database
R1833	T2904	T2903	punct	;,November
R1834	T2905	T2903	nmod	October,November
R1835	T2906	T2903	punct	/,November
R1836	T2907	T2903	nummod	2004,November
R1837	T2908	T2903	punct	),November
R1838	T2909	T2910	punct	(,28
R1839	T2910	T2891	parataxis	28,Database
R1840	T2911	T2910	punct	),28
R1841	T2912	T2865	punct	.,obtained
R1842	T2931	T2989	appos	TTCCGATGTGCCAAGTGTGGC,Csrp1
R1843	T2932	T2933	compound	RT,PCR
R1844	T2934	T2933	punct	–,PCR
R1845	T2936	T2937	det	All,PCRs
R1846	T2937	T2940	nsubjpass	PCRs,performed
R1847	T2938	T2937	compound	RT,PCRs
R1848	T2939	T2937	punct	–,PCRs
R1849	T2941	T2940	auxpass	were,performed
R1850	T2942	T2940	prep	with,performed
R1851	T2943	T2944	compound	QIAGEN®,Kit
R1852	T2944	T2942	pobj	Kit,with
R1853	T2945	T2944	compound	OneStep,Kit
R1854	T2946	T2947	compound	RT,PCR
R1855	T2947	T2944	compound	PCR,Kit
R1856	T2948	T2947	punct	–,PCR
R1857	T2949	T2950	punct	(,QIAGEN
R1858	T2950	T2944	parataxis	QIAGEN,Kit
R1859	T2951	T2950	punct	),QIAGEN
R1860	T2952	T2940	prep	according,performed
R1861	T2953	T2952	prep	to,according
R1862	T2954	T2955	det	the,manufacturer
R1863	T2955	T2956	poss	manufacturer,instructions
R1864	T2956	T2953	pobj	instructions,to
R1865	T2957	T2955	case	's,manufacturer
R1866	T2958	T2940	punct	", ",performed
R1867	T2959	T2940	advcl	using,performed
R1868	T2960	T2961	quantmod	150,200
R1869	T2961	T2963	nummod	200,ng
R1870	T2962	T2961	punct	–,200
R1871	T2963	T2964	nmod	ng,RNA
R1872	T2964	T2959	dobj	RNA,using
R1873	T2965	T2966	compound	DNase,I
R1874	T2966	T2967	npadvmod	I,digested
R1875	T2967	T2964	amod	digested,RNA
R1876	T2968	T2967	punct	-,digested
R1877	T2969	T2964	amod	total,RNA
R1878	T2970	T2971	punct	(,isolation
R1879	T2971	T2959	parataxis	isolation,using
R1880	T2972	T2971	compound	RNA,isolation
R1881	T2973	T2971	advcl	see,isolation
R1882	T2974	T2975	compound	microarray,analysis
R1883	T2975	T2973	dobj	analysis,see
R1884	T2976	T2971	punct	),isolation
R1885	T2977	T2940	punct	.,performed
R1886	T2979	T2980	det	The,reactions
R1887	T2980	T2981	nsubjpass	reactions,carried
R1888	T2982	T2981	auxpass	were,carried
R1889	T2983	T2981	prt	out,carried
R1890	T2984	T2981	advcl	using,carried
R1891	T2985	T2986	det	the,primers
R1892	T2986	T2984	dobj	primers,using
R1893	T2987	T2986	amod	following,primers
R1894	T2988	T2986	punct	: ,primers
R1895	T2989	T2986	appos	Csrp1,primers
R1896	T2990	T2989	punct	: ,Csrp1
R1897	T2991	T2931	nummod	5,TTCCGATGTGCCAAGTGTGGC
R1898	T2992	T2991	punct	′,5
R1899	T2993	T2931	punct	-,TTCCGATGTGCCAAGTGTGGC
R1900	T2994	T2931	punct	-,TTCCGATGTGCCAAGTGTGGC
R1901	T2995	T2931	nummod	3,TTCCGATGTGCCAAGTGTGGC
R1902	T2996	T2931	punct	′,TTCCGATGTGCCAAGTGTGGC
R1903	T2997	T2931	punct	;,TTCCGATGTGCCAAGTGTGGC
R1904	T2998	T2999	nummod	5,AGTAGAGAGTGGACATTCAGC
R1905	T2999	T2931	appos	AGTAGAGAGTGGACATTCAGC,TTCCGATGTGCCAAGTGTGGC
R1906	T3000	T2998	punct	′,5
R1907	T3001	T2999	punct	-,AGTAGAGAGTGGACATTCAGC
R1908	T3002	T2999	punct	-,AGTAGAGAGTGGACATTCAGC
R1909	T3003	T2999	nummod	3,AGTAGAGAGTGGACATTCAGC
R1910	T3004	T2999	punct	′,AGTAGAGAGTGGACATTCAGC
R1911	T3005	T2989	punct	", ",Csrp1
R1912	T3006	T3007	compound	hypoxanthin,phosphoribosyltransferase
R1913	T3007	T2989	appos	phosphoribosyltransferase,Csrp1
R1914	T3008	T3007	punct	-,phosphoribosyltransferase
R1915	T3009	T3007	compound	guanin,phosphoribosyltransferase
R1916	T3010	T3007	punct	-,phosphoribosyltransferase
R1917	T3011	T3007	punct	(,phosphoribosyltransferase
R1918	T3012	T3007	appos	Hprt,phosphoribosyltransferase
R1919	T3013	T3007	punct	),phosphoribosyltransferase
R1920	T3014	T3007	punct	: ,phosphoribosyltransferase
R1921	T3015	T3016	nummod	5,GCTGGTGAAAAGGACCTCTCG
R1922	T3016	T3007	appos	GCTGGTGAAAAGGACCTCTCG,phosphoribosyltransferase
R1923	T3017	T3015	punct	′,5
R1924	T3018	T3016	punct	-,GCTGGTGAAAAGGACCTCTCG
R1925	T3019	T3016	punct	-,GCTGGTGAAAAGGACCTCTCG
R1926	T3020	T3016	nummod	3,GCTGGTGAAAAGGACCTCTCG
R1927	T3021	T3016	punct	′,GCTGGTGAAAAGGACCTCTCG
R1928	T3022	T3016	punct	;,GCTGGTGAAAAGGACCTCTCG
R1929	T3023	T3024	nummod	5,CCACAGGACTAGGACACCTGC
R1930	T3024	T3016	appos	CCACAGGACTAGGACACCTGC,GCTGGTGAAAAGGACCTCTCG
R1931	T3025	T3023	punct	′,5
R1932	T3026	T3024	punct	-,CCACAGGACTAGGACACCTGC
R1933	T3027	T3024	punct	-,CCACAGGACTAGGACACCTGC
R1934	T3028	T3024	nummod	3,CCACAGGACTAGGACACCTGC
R1935	T3029	T3024	punct	′,CCACAGGACTAGGACACCTGC
R1936	T3030	T2989	punct	", ",Csrp1
R1937	T3031	T2989	appos	Mtf1,Csrp1
R1938	T3032	T3031	punct	: ,Mtf1
R1939	T3033	T3034	nummod	5,GTGACTTTTGAGACTGTACTGAGTG
R1940	T3034	T3031	appos	GTGACTTTTGAGACTGTACTGAGTG,Mtf1
R1941	T3035	T3033	punct	′,5
R1942	T3036	T3034	punct	-,GTGACTTTTGAGACTGTACTGAGTG
R1943	T3037	T3034	punct	-,GTGACTTTTGAGACTGTACTGAGTG
R1944	T3038	T3034	nummod	3,GTGACTTTTGAGACTGTACTGAGTG
R1945	T3039	T3034	punct	′,GTGACTTTTGAGACTGTACTGAGTG
R1946	T3040	T3034	punct	;,GTGACTTTTGAGACTGTACTGAGTG
R1947	T3041	T3042	nummod	5,CATGCCAAGAAACATTGAAGGTG
R1948	T3042	T3034	appos	CATGCCAAGAAACATTGAAGGTG,GTGACTTTTGAGACTGTACTGAGTG
R1949	T3043	T3041	punct	′,5
R1950	T3044	T3042	punct	-,CATGCCAAGAAACATTGAAGGTG
R1951	T3045	T3042	punct	-,CATGCCAAGAAACATTGAAGGTG
R1952	T3046	T3042	nummod	3,CATGCCAAGAAACATTGAAGGTG
R1953	T3047	T3042	punct	′,CATGCCAAGAAACATTGAAGGTG
R1954	T3048	T2989	punct	", ",Csrp1
R1955	T3049	T2989	appos	Ndrg1,Csrp1
R1956	T3050	T3049	punct	: ,Ndrg1
R1957	T3051	T3052	nummod	5,AGATACACAACAACGTGGAGG
R1958	T3052	T3049	appos	AGATACACAACAACGTGGAGG,Ndrg1
R1959	T3053	T3051	punct	′,5
R1960	T3054	T3052	punct	-,AGATACACAACAACGTGGAGG
R1961	T3055	T3052	punct	-,AGATACACAACAACGTGGAGG
R1962	T3056	T3052	nummod	3,AGATACACAACAACGTGGAGG
R1963	T3057	T3052	punct	′,AGATACACAACAACGTGGAGG
R1964	T3058	T3052	punct	;,AGATACACAACAACGTGGAGG
R1965	T3059	T3060	nummod	5,TGTGCGAGCGGCTTCGGGGGC
R1966	T3060	T3052	appos	TGTGCGAGCGGCTTCGGGGGC,AGATACACAACAACGTGGAGG
R1967	T3061	T3059	punct	′,5
R1968	T3062	T3060	punct	-,TGTGCGAGCGGCTTCGGGGGC
R1969	T3063	T3060	punct	-,TGTGCGAGCGGCTTCGGGGGC
R1970	T3064	T3060	nummod	3,TGTGCGAGCGGCTTCGGGGGC
R1971	T3065	T3060	punct	′,TGTGCGAGCGGCTTCGGGGGC
R1972	T3066	T2989	punct	", ",Csrp1
R1973	T3067	T2989	appos	Sepw1,Csrp1
R1974	T3068	T3067	punct	: ,Sepw1
R1975	T3069	T3070	nummod	5,TAGAGGCAGGGTCCTGAAAGC
R1976	T3070	T3067	appos	TAGAGGCAGGGTCCTGAAAGC,Sepw1
R1977	T3071	T3069	punct	′,5
R1978	T3072	T3070	punct	-,TAGAGGCAGGGTCCTGAAAGC
R1979	T3073	T3070	punct	-,TAGAGGCAGGGTCCTGAAAGC
R1980	T3074	T3070	nummod	3,TAGAGGCAGGGTCCTGAAAGC
R1981	T3075	T3070	punct	′,TAGAGGCAGGGTCCTGAAAGC
R1982	T3076	T3070	punct	;,TAGAGGCAGGGTCCTGAAAGC
R1983	T3077	T3078	nummod	5,ACACCTGGAAACATGGCTGCC
R1984	T3078	T3070	appos	ACACCTGGAAACATGGCTGCC,TAGAGGCAGGGTCCTGAAAGC
R1985	T3079	T3077	punct	′,5
R1986	T3080	T3078	punct	-,ACACCTGGAAACATGGCTGCC
R1987	T3081	T3078	punct	-,ACACCTGGAAACATGGCTGCC
R1988	T3082	T3078	nummod	3,ACACCTGGAAACATGGCTGCC
R1989	T3083	T3078	punct	′,ACACCTGGAAACATGGCTGCC
R1990	T3084	T2989	punct	", ",Csrp1
R1991	T3085	T2989	appos	Slc39a10,Csrp1
R1992	T3086	T3085	punct	: ,Slc39a10
R1993	T3087	T3088	nummod	5,GCTGTGGCTGGTAGTAAAAGC
R1994	T3088	T3085	appos	GCTGTGGCTGGTAGTAAAAGC,Slc39a10
R1995	T3089	T3087	punct	′,5
R1996	T3090	T3088	punct	-,GCTGTGGCTGGTAGTAAAAGC
R1997	T3091	T3088	punct	-,GCTGTGGCTGGTAGTAAAAGC
R1998	T3092	T3088	nummod	3,GCTGTGGCTGGTAGTAAAAGC
R1999	T3093	T3088	punct	′,GCTGTGGCTGGTAGTAAAAGC
R2000	T3094	T3088	punct	;,GCTGTGGCTGGTAGTAAAAGC
R2001	T3095	T3096	nummod	5,GTGGCATGGGATGTAAACAGC
R2002	T3096	T3088	appos	GTGGCATGGGATGTAAACAGC,GCTGTGGCTGGTAGTAAAAGC
R2003	T3097	T3095	punct	′,5
R2004	T3098	T3096	punct	-,GTGGCATGGGATGTAAACAGC
R2005	T3099	T3096	punct	-,GTGGCATGGGATGTAAACAGC
R2006	T3100	T3096	nummod	3,GTGGCATGGGATGTAAACAGC
R2007	T3101	T3096	punct	′,GTGGCATGGGATGTAAACAGC
R2008	T3102	T2981	punct	.,carried
R2009	T3137	T3138	compound	S1,mapping
R2010	T3139	T3138	compound	nuclease,mapping
R2011	T3140	T3138	prep	of,mapping
R2012	T3141	T3140	pobj	transcripts,of
R2013	T3142	T3143	punct	(,analysis
R2014	T3143	T3138	parataxis	analysis,mapping
R2015	T3144	T3143	compound	S1,analysis
R2016	T3145	T3143	punct	),analysis
R2017	T3147	T3148	compound	S1,analysis
R2018	T3148	T3149	nsubjpass	analysis,performed
R2019	T3150	T3149	auxpass	was,performed
R2020	T3151	T3152	mark	as,described
R2021	T3152	T3149	advcl	described,performed
R2022	T3153	T3152	advmod	previously,described
R2023	T3154	T3155	punct	(,29
R2024	T3155	T3152	parataxis	29,described
R2025	T3156	T3155	punct	),29
R2026	T3157	T3149	punct	", ",performed
R2027	T3158	T3149	advcl	using,performed
R2028	T3159	T3160	nummod	100,µg
R2029	T3160	T3161	nmod	µg,RNA
R2030	T3161	T3158	dobj	RNA,using
R2031	T3162	T3163	npadvmod	DNase,digested
R2032	T3163	T3161	amod	digested,RNA
R2033	T3164	T3162	nummod	I,DNase
R2034	T3165	T3163	punct	-,digested
R2035	T3166	T3161	amod	total,RNA
R2036	T3167	T3168	punct	(,see
R2037	T3168	T3161	parataxis	see,RNA
R2038	T3169	T3170	compound	RNA,isolation
R2039	T3170	T3168	nsubj	isolation,see
R2040	T3171	T3172	compound	microarray,analysis
R2041	T3172	T3168	dobj	analysis,see
R2042	T3173	T3168	punct	),see
R2043	T3174	T3149	punct	.,performed
R2044	T3176	T3177	det	The,gels
R2045	T3177	T3178	nsubjpass	gels,developed
R2046	T3179	T3178	auxpass	were,developed
R2047	T3180	T3178	advcl	using,developed
R2048	T3181	T3180	dobj	PhosphorImager,using
R2049	T3182	T3183	punct	(,Dynamics
R2050	T3183	T3178	parataxis	Dynamics,developed
R2051	T3184	T3183	compound	Molecular,Dynamics
R2052	T3185	T3183	punct	),Dynamics
R2053	T3186	T3178	punct	.,developed
R2054	T3188	T3189	compound	S1,analysis
R2055	T3189	T3190	nsubjpass	analysis,done
R2056	T3191	T3190	auxpass	was,done
R2057	T3192	T3190	prep	with,done
R2058	T3193	T3194	det	the,oligonucleotides
R2059	T3194	T3192	pobj	oligonucleotides,with
R2060	T3195	T3194	amod	following,oligonucleotides
R2061	T3196	T3197	npadvmod	32P,labeled
R2062	T3197	T3194	amod	labeled,oligonucleotides
R2063	T3198	T3197	punct	-,labeled
R2064	T3199	T3194	punct	: ,oligonucleotides
R2065	T3200	T3201	compound	Hprt,S1
R2066	T3201	T3194	appos	S1,oligonucleotides
R2067	T3202	T3201	punct	: ,S1
R2068	T3203	T3204	nummod	5,TCTTCAGTCTGATAAAATCTACAGTCATAGGAATGGATCTATCACTATTTCTATTCAGTGATTACATTAAAG
R2069	T3204	T3201	appos	TCTTCAGTCTGATAAAATCTACAGTCATAGGAATGGATCTATCACTATTTCTATTCAGTGATTACATTAAAG,S1
R2070	T3205	T3203	punct	′,5
R2071	T3206	T3204	punct	-,TCTTCAGTCTGATAAAATCTACAGTCATAGGAATGGATCTATCACTATTTCTATTCAGTGATTACATTAAAG
R2072	T3207	T3204	punct	-,TCTTCAGTCTGATAAAATCTACAGTCATAGGAATGGATCTATCACTATTTCTATTCAGTGATTACATTAAAG
R2073	T3208	T3204	nummod	3,TCTTCAGTCTGATAAAATCTACAGTCATAGGAATGGATCTATCACTATTTCTATTCAGTGATTACATTAAAG
R2074	T3209	T3204	punct	′,TCTTCAGTCTGATAAAATCTACAGTCATAGGAATGGATCTATCACTATTTCTATTCAGTGATTACATTAAAG
R2075	T3210	T3201	punct	", ",S1
R2076	T3211	T3212	compound	Sepw1,S1
R2077	T3212	T3201	appos	S1,S1
R2078	T3213	T3212	punct	: ,S1
R2079	T3214	T3215	nummod	5,TTCAACCGGGAACACCTGGAAACATGGCTGCCTGTCTTCTTGAAGTCTTGAGGTGGAAAGGGAAAGCAAAGCAGGAGGGTTTTCCCACCC
R2080	T3215	T3212	appos	TTCAACCGGGAACACCTGGAAACATGGCTGCCTGTCTTCTTGAAGTCTTGAGGTGGAAAGGGAAAGCAAAGCAGGAGGGTTTTCCCACCC,S1
R2081	T3216	T3214	punct	′,5
R2082	T3217	T3215	punct	-,TTCAACCGGGAACACCTGGAAACATGGCTGCCTGTCTTCTTGAAGTCTTGAGGTGGAAAGGGAAAGCAAAGCAGGAGGGTTTTCCCACCC
R2083	T3218	T3215	punct	-,TTCAACCGGGAACACCTGGAAACATGGCTGCCTGTCTTCTTGAAGTCTTGAGGTGGAAAGGGAAAGCAAAGCAGGAGGGTTTTCCCACCC
R2084	T3219	T3215	nummod	3,TTCAACCGGGAACACCTGGAAACATGGCTGCCTGTCTTCTTGAAGTCTTGAGGTGGAAAGGGAAAGCAAAGCAGGAGGGTTTTCCCACCC
R2085	T3220	T3215	punct	′,TTCAACCGGGAACACCTGGAAACATGGCTGCCTGTCTTCTTGAAGTCTTGAGGTGGAAAGGGAAAGCAAAGCAGGAGGGTTTTCCCACCC
R2086	T3221	T3190	punct	.,done
R2089	T3351	T3352	amod	Electrophoretic,shift
R2090	T3352	T3354	compound	shift,assay
R2091	T3353	T3352	compound	mobility,shift
R2092	T3355	T3354	punct	(,assay
R2093	T3356	T3354	appos	EMSA,assay
R2094	T3357	T3354	punct	),assay
R2095	T3359	T3360	nsubjpass	Protein,extracted
R2096	T3361	T3360	auxpass	was,extracted
R2097	T3362	T3360	prep	from,extracted
R2098	T3363	T3364	compound	liver,tissue
R2099	T3364	T3362	pobj	tissue,from
R2100	T3365	T3360	prep	with,extracted
R2101	T3366	T3367	compound	T,PER
R2102	T3367	T3365	pobj	PER,with
R2103	T3368	T3367	punct	-,PER
R2104	T3369	T3367	punct	™,PER
R2105	T3370	T3371	compound	Tissue,Protein
R2106	T3371	T3372	compound	Protein,Extraction
R2107	T3372	T3373	compound	Extraction,Reagent
R2108	T3373	T3367	appos	Reagent,PER
R2109	T3374	T3375	punct	(,Pierce
R2110	T3375	T3367	parataxis	Pierce,PER
R2111	T3376	T3375	punct	),Pierce
R2112	T3377	T3360	prep	according,extracted
R2113	T3378	T3377	prep	to,according
R2114	T3379	T3380	det	the,manufacturer
R2115	T3380	T3381	poss	manufacturer,instructions
R2116	T3381	T3378	pobj	instructions,to
R2117	T3382	T3380	case	's,manufacturer
R2118	T3383	T3360	punct	", ",extracted
R2119	T3384	T3360	advcl	using,extracted
R2120	T3385	T3386	det	a,ratio
R2121	T3386	T3384	dobj	ratio,using
R2122	T3387	T3386	nummod	1,ratio
R2123	T3388	T3389	punct	:,10
R2124	T3389	T3387	prep	10,1
R2125	T3390	T3386	prep	of,ratio
R2126	T3391	T3392	compound	mouse,tissue
R2127	T3392	T3390	pobj	tissue,of
R2128	T3393	T3394	punct	(,mg
R2129	T3394	T3392	parataxis	mg,tissue
R2130	T3395	T3394	punct	),mg
R2131	T3396	T3386	prep	to,ratio
R2132	T3397	T3398	compound	T,PER
R2133	T3398	T3400	compound	PER,reagent
R2134	T3399	T3398	punct	-,PER
R2135	T3400	T3396	pobj	reagent,to
R2136	T3401	T3402	punct	(,µl
R2137	T3402	T3400	parataxis	µl,reagent
R2138	T3403	T3402	punct	),µl
R2139	T3404	T3360	punct	.,extracted
R2140	T3406	T3407	nsubjpass	EMSA,performed
R2141	T3408	T3407	auxpass	was,performed
R2142	T3409	T3407	advmod	essentially,performed
R2143	T3410	T3411	mark	as,described
R2144	T3411	T3407	advcl	described,performed
R2145	T3412	T3411	advmod	previously,described
R2146	T3413	T3414	punct	(,10
R2147	T3414	T3411	parataxis	10,described
R2148	T3415	T3414	punct	),10
R2149	T3416	T3407	punct	.,performed
R2150	T3418	T3419	det	All,reactions
R2151	T3419	T3421	nsubjpass	reactions,carried
R2152	T3420	T3419	compound	binding,reactions
R2153	T3422	T3421	auxpass	were,carried
R2154	T3423	T3421	prt	out,carried
R2155	T3424	T3421	prep	by,carried
R2156	T3425	T3424	pcomp	incubating,by
R2157	T3426	T3427	quantmod	2,5
R2158	T3427	T3429	nummod	5,fmol
R2159	T3428	T3427	punct	–,5
R2160	T3429	T3430	nmod	fmol,oligonucleotides
R2162	T3431	T3432	npadvmod	32P,labeled
R2163	T3432	T3430	amod	labeled,oligonucleotides
R2164	T3433	T3432	punct	-,labeled
R2165	T3434	T3432	npadvmod	end,labeled
R2166	T3435	T3432	punct	-,labeled
R2167	T3436	T3437	amod	double,stranded
R2168	T3437	T3430	amod	stranded,oligonucleotides
R2169	T3438	T3437	punct	-,stranded
R2170	T3439	T3425	prep	with,incubating
R2171	T3440	T3441	quantmod	100,130
R2172	T3441	T3443	nummod	130,µg
R2173	T3442	T3441	quantmod	to,130
R2174	T3443	T3444	compound	µg,extract
R2175	T3444	T3439	pobj	extract,with
R2176	T3445	T3446	compound	liver,protein
R2177	T3446	T3444	compound	protein,extract
R2178	T3447	T3421	punct	.,carried
R2179	T3449	T3450	prep	For,added
R2180	T3451	T3452	compound	competition,experiments
R2181	T3452	T3449	pobj	experiments,For
R2182	T3453	T3450	punct	", ",added
R2183	T3454	T3455	nummod	5,pmol
R2184	T3455	T3450	nsubjpass	pmol,added
R2185	T3456	T3455	prep	of,pmol
R2186	T3457	T3458	amod	unlabeled,oligonucleotide
R2187	T3458	T3456	pobj	oligonucleotide,of
R2188	T3459	T3458	compound	competitor,oligonucleotide
R2189	T3460	T3450	auxpass	was,added
R2190	T3461	T3450	prep	to,added
R2191	T3462	T3463	det	the,reaction
R2192	T3463	T3461	pobj	reaction,to
R2193	T3464	T3463	compound	binding,reaction
R2194	T3465	T3450	prep	before,added
R2195	T3466	T3465	pobj	addition,before
R2196	T3467	T3466	prep	of,addition
R2197	T3468	T3469	det	the,extract
R2198	T3469	T3467	pobj	extract,of
R2199	T3470	T3450	punct	.,added
R2200	T3472	T3473	det	All,gels
R2201	T3473	T3475	nsubjpass	gels,developed
R2202	T3474	T3473	compound	EMSA,gels
R2203	T3476	T3475	auxpass	were,developed
R2204	T3477	T3475	advcl	using,developed
R2205	T3478	T3477	dobj	PhosphorImager,using
R2206	T3479	T3480	punct	(,Dynamics
R2207	T3480	T3478	parataxis	Dynamics,PhosphorImager
R2208	T3481	T3480	compound	Molecular,Dynamics
R2209	T3482	T3480	punct	),Dynamics
R2210	T3483	T3475	punct	.,developed
R2211	T3485	T3486	det	The,oligonucleotides
R2212	T3486	T3488	nsubjpass	oligonucleotides,annealed
R2213	T3487	T3486	amod	following,oligonucleotides
R2214	T3489	T3488	auxpass	were,annealed
R2215	T3490	T3488	cc	and,annealed
R2216	T3491	T3488	conj	used,annealed
R2217	T3492	T3491	prep	for,used
R2218	T3493	T3494	det	the,reactions
R2219	T3494	T3492	pobj	reactions,for
R2220	T3495	T3491	punct	: ,used
R2221	T3496	T3497	compound	Csrp1,MRE1
R2222	T3497	T3491	dobj	MRE1,used
R2223	T3498	T3497	punct	: ,MRE1
R2224	T3499	T3500	nummod	5,GGAAACAAAACGGCGCGCACTCCGGCGC
R2225	T3500	T3497	appos	GGAAACAAAACGGCGCGCACTCCGGCGC,MRE1
R2226	T3501	T3499	punct	′,5
R2227	T3502	T3500	punct	-,GGAAACAAAACGGCGCGCACTCCGGCGC
R2228	T3503	T3500	punct	-,GGAAACAAAACGGCGCGCACTCCGGCGC
R2229	T3504	T3500	nummod	3,GGAAACAAAACGGCGCGCACTCCGGCGC
R2230	T3505	T3500	punct	′,GGAAACAAAACGGCGCGCACTCCGGCGC
R2231	T3506	T3500	punct	;,GGAAACAAAACGGCGCGCACTCCGGCGC
R2232	T3507	T3508	nummod	5,CGGAGTGCGCGCCGTTTTGT
R2233	T3508	T3500	appos	CGGAGTGCGCGCCGTTTTGT,GGAAACAAAACGGCGCGCACTCCGGCGC
R2234	T3509	T3507	punct	′,5
R2235	T3510	T3508	punct	-,CGGAGTGCGCGCCGTTTTGT
R2236	T3511	T3508	compound	GGCTGCGC,CGGAGTGCGCGCCGTTTTGT
R2237	T3512	T3508	punct	-,CGGAGTGCGCGCCGTTTTGT
R2238	T3513	T3508	nummod	3,CGGAGTGCGCGCCGTTTTGT
R2239	T3514	T3508	punct	′,CGGAGTGCGCGCCGTTTTGT
R2240	T3515	T3497	punct	", ",MRE1
R2241	T3516	T3517	compound	Csrp1,MRE2
R2242	T3517	T3497	appos	MRE2,MRE1
R2243	T3518	T3517	punct	: ,MRE2
R2244	T3519	T3520	nummod	5,TGTTGTGGTGCAGTGTGCAAAGCCTAC
R2245	T3520	T3517	appos	TGTTGTGGTGCAGTGTGCAAAGCCTAC,MRE2
R2246	T3521	T3519	punct	′,5
R2247	T3522	T3520	punct	-,TGTTGTGGTGCAGTGTGCAAAGCCTAC
R2248	T3523	T3520	punct	-,TGTTGTGGTGCAGTGTGCAAAGCCTAC
R2249	T3524	T3520	nummod	3,TGTTGTGGTGCAGTGTGCAAAGCCTAC
R2250	T3525	T3520	punct	′,TGTTGTGGTGCAGTGTGCAAAGCCTAC
R2251	T3526	T3520	punct	;,TGTTGTGGTGCAGTGTGCAAAGCCTAC
R2252	T3527	T3528	nummod	5,ACCAGTAGGCTTTGCACACTGCACCAC
R2253	T3528	T3520	appos	ACCAGTAGGCTTTGCACACTGCACCAC,TGTTGTGGTGCAGTGTGCAAAGCCTAC
R2254	T3529	T3527	punct	′,5
R2255	T3530	T3528	punct	-,ACCAGTAGGCTTTGCACACTGCACCAC
R2256	T3531	T3528	punct	-,ACCAGTAGGCTTTGCACACTGCACCAC
R2257	T3532	T3528	nummod	3,ACCAGTAGGCTTTGCACACTGCACCAC
R2258	T3533	T3528	punct	′,ACCAGTAGGCTTTGCACACTGCACCAC
R2259	T3534	T3497	punct	", ",MRE1
R2260	T3535	T3536	compound	Csrp1,MRE3
R2261	T3536	T3497	appos	MRE3,MRE1
R2262	T3537	T3536	punct	: ,MRE3
R2263	T3538	T3539	nummod	5,GAGATCGCCATAGGGTGCAAAGAGAAG
R2264	T3539	T3536	appos	GAGATCGCCATAGGGTGCAAAGAGAAG,MRE3
R2265	T3540	T3538	punct	′,5
R2266	T3541	T3539	punct	-,GAGATCGCCATAGGGTGCAAAGAGAAG
R2267	T3542	T3539	punct	-,GAGATCGCCATAGGGTGCAAAGAGAAG
R2268	T3543	T3539	nummod	3,GAGATCGCCATAGGGTGCAAAGAGAAG
R2269	T3544	T3539	punct	′,GAGATCGCCATAGGGTGCAAAGAGAAG
R2270	T3545	T3539	punct	;,GAGATCGCCATAGGGTGCAAAGAGAAG
R2271	T3546	T3547	nummod	5,GTGACTTCTCTTTGCACCCTATGGCGA
R2272	T3547	T3539	appos	GTGACTTCTCTTTGCACCCTATGGCGA,GAGATCGCCATAGGGTGCAAAGAGAAG
R2273	T3548	T3546	punct	′,5
R2274	T3549	T3547	punct	-,GTGACTTCTCTTTGCACCCTATGGCGA
R2275	T3550	T3547	punct	-,GTGACTTCTCTTTGCACCCTATGGCGA
R2276	T3551	T3547	nummod	3,GTGACTTCTCTTTGCACCCTATGGCGA
R2277	T3552	T3547	punct	′,GTGACTTCTCTTTGCACCCTATGGCGA
R2278	T3553	T3497	punct	", ",MRE1
R2279	T3554	T3555	compound	Csrp1,MRE4
R2280	T3555	T3497	appos	MRE4,MRE1
R2281	T3556	T3555	punct	: ,MRE4
R2282	T3557	T3558	nummod	5,TGTCTTATTCTGGAGTGCAAGTTAGTC
R2283	T3558	T3555	appos	TGTCTTATTCTGGAGTGCAAGTTAGTC,MRE4
R2284	T3559	T3557	punct	′,5
R2285	T3560	T3558	punct	-,TGTCTTATTCTGGAGTGCAAGTTAGTC
R2286	T3561	T3558	punct	-,TGTCTTATTCTGGAGTGCAAGTTAGTC
R2287	T3562	T3558	nummod	3,TGTCTTATTCTGGAGTGCAAGTTAGTC
R2288	T3563	T3558	punct	′,TGTCTTATTCTGGAGTGCAAGTTAGTC
R2289	T3564	T3558	punct	;,TGTCTTATTCTGGAGTGCAAGTTAGTC
R2290	T3565	T3566	nummod	5,AGGGGACTAACTTGCACTCCAGAATAA
R2291	T3566	T3558	appos	AGGGGACTAACTTGCACTCCAGAATAA,TGTCTTATTCTGGAGTGCAAGTTAGTC
R2292	T3567	T3565	punct	′,5
R2293	T3568	T3566	punct	-,AGGGGACTAACTTGCACTCCAGAATAA
R2294	T3569	T3566	punct	-,AGGGGACTAACTTGCACTCCAGAATAA
R2295	T3570	T3566	nummod	3,AGGGGACTAACTTGCACTCCAGAATAA
R2296	T3571	T3566	punct	′,AGGGGACTAACTTGCACTCCAGAATAA
R2297	T3572	T3497	punct	", ",MRE1
R2298	T3573	T3497	appos	Gal4,MRE1
R2299	T3574	T3573	punct	: ,Gal4
R2300	T3575	T3576	nummod	5,TCCGGAGGACTGTCCTCCGG
R2301	T3576	T3573	appos	TCCGGAGGACTGTCCTCCGG,Gal4
R2302	T3577	T3575	punct	′,5
R2303	T3578	T3576	punct	-,TCCGGAGGACTGTCCTCCGG
R2304	T3579	T3576	punct	-,TCCGGAGGACTGTCCTCCGG
R2305	T3580	T3576	nummod	3,TCCGGAGGACTGTCCTCCGG
R2306	T3581	T3576	punct	′,TCCGGAGGACTGTCCTCCGG
R2307	T3582	T3576	punct	;,TCCGGAGGACTGTCCTCCGG
R2308	T3583	T3584	nummod	5,GCCGGAGGACAGTCCTCCGG
R2309	T3584	T3576	appos	GCCGGAGGACAGTCCTCCGG,TCCGGAGGACTGTCCTCCGG
R2310	T3585	T3583	punct	′,5
R2311	T3586	T3584	punct	-,GCCGGAGGACAGTCCTCCGG
R2312	T3587	T3584	punct	-,GCCGGAGGACAGTCCTCCGG
R2313	T3588	T3584	nummod	3,GCCGGAGGACAGTCCTCCGG
R2314	T3589	T3584	punct	′,GCCGGAGGACAGTCCTCCGG
R2315	T3590	T3497	punct	", ",MRE1
R2316	T3591	T3497	appos	MREd,MRE1
R2317	T3592	T3591	punct	[,MREd
R2318	T3593	T3591	appos	MRE,MREd
R2319	T3594	T3593	acl	derived,MRE
R2320	T3595	T3594	prep	from,derived
R2321	T3596	T3597	compound	mouse,promoter
R2322	T3597	T3595	pobj	promoter,from
R2323	T3598	T3597	compound	Mt1,promoter
R2324	T3599	T3600	punct	(,10
R2325	T3600	T3594	parataxis	10,derived
R2326	T3601	T3600	punct	),10
R2327	T3602	T3591	punct	],MREd
R2328	T3603	T3591	punct	: ,MREd
R2329	T3604	T3605	nummod	5,CGAGGGAGCTCTGCACTCCGCCCGAAAAGTG
R2330	T3605	T3591	appos	CGAGGGAGCTCTGCACTCCGCCCGAAAAGTG,MREd
R2331	T3606	T3604	punct	′,5
R2332	T3607	T3605	punct	-,CGAGGGAGCTCTGCACTCCGCCCGAAAAGTG
R2333	T3608	T3605	punct	-,CGAGGGAGCTCTGCACTCCGCCCGAAAAGTG
R2334	T3609	T3605	nummod	3,CGAGGGAGCTCTGCACTCCGCCCGAAAAGTG
R2335	T3610	T3605	punct	′,CGAGGGAGCTCTGCACTCCGCCCGAAAAGTG
R2336	T3611	T3605	punct	;,CGAGGGAGCTCTGCACTCCGCCCGAAAAGTG
R2337	T3612	T3613	nummod	5,TCGACACTTTTCGGGCGGAGTGCAGAGCTCCCTCGAGCT
R2338	T3613	T3605	appos	TCGACACTTTTCGGGCGGAGTGCAGAGCTCCCTCGAGCT,CGAGGGAGCTCTGCACTCCGCCCGAAAAGTG
R2339	T3614	T3612	punct	′,5
R2340	T3615	T3613	punct	-,TCGACACTTTTCGGGCGGAGTGCAGAGCTCCCTCGAGCT
R2341	T3616	T3613	punct	-,TCGACACTTTTCGGGCGGAGTGCAGAGCTCCCTCGAGCT
R2342	T3617	T3613	nummod	3,TCGACACTTTTCGGGCGGAGTGCAGAGCTCCCTCGAGCT
R2343	T3618	T3613	punct	′,TCGACACTTTTCGGGCGGAGTGCAGAGCTCCCTCGAGCT
R2344	T3619	T3497	punct	", ",MRE1
R2345	T3620	T3621	compound	MRE,s
R2346	T3621	T3497	appos	s,MRE1
R2347	T3622	T3621	punct	-,s
R2348	T3623	T3621	punct	[,s
R2349	T3624	T3625	amod	synthetic,sequence
R2350	T3625	T3621	appos	sequence,s
R2351	T3626	T3625	compound	MRE,sequence
R2352	T3627	T3625	compound	consensus,sequence
R2353	T3628	T3629	punct	(,10
R2354	T3629	T3625	parataxis	10,sequence
R2355	T3630	T3629	punct	),10
R2356	T3631	T3621	punct	],s
R2357	T3632	T3621	punct	: ,s
R2358	T3633	T3634	nummod	5,CGAGGGAGCTCTGCACACGGCCCGAAAAGTG
R2359	T3634	T3621	appos	CGAGGGAGCTCTGCACACGGCCCGAAAAGTG,s
R2360	T3635	T3633	punct	′,5
R2361	T3636	T3634	punct	-,CGAGGGAGCTCTGCACACGGCCCGAAAAGTG
R2362	T3637	T3634	punct	-,CGAGGGAGCTCTGCACACGGCCCGAAAAGTG
R2363	T3638	T3634	nummod	3,CGAGGGAGCTCTGCACACGGCCCGAAAAGTG
R2364	T3639	T3634	punct	′,CGAGGGAGCTCTGCACACGGCCCGAAAAGTG
R2365	T3640	T3634	punct	;,CGAGGGAGCTCTGCACACGGCCCGAAAAGTG
R2366	T3641	T3642	nummod	5,TCGACACTTTTCGGGCCGTGTGCAGAGCTCCCTCGAGCT
R2367	T3642	T3634	appos	TCGACACTTTTCGGGCCGTGTGCAGAGCTCCCTCGAGCT,CGAGGGAGCTCTGCACACGGCCCGAAAAGTG
R2368	T3643	T3641	punct	′,5
R2369	T3644	T3642	punct	-,TCGACACTTTTCGGGCCGTGTGCAGAGCTCCCTCGAGCT
R2370	T3645	T3642	punct	-,TCGACACTTTTCGGGCCGTGTGCAGAGCTCCCTCGAGCT
R2371	T3646	T3642	nummod	3,TCGACACTTTTCGGGCCGTGTGCAGAGCTCCCTCGAGCT
R2372	T3647	T3642	punct	′,TCGACACTTTTCGGGCCGTGTGCAGAGCTCCCTCGAGCT
R2373	T3648	T3497	punct	", ",MRE1
R2374	T3649	T3650	compound	Ndrg1,MRE1
R2375	T3650	T3497	appos	MRE1,MRE1
R2376	T3651	T3650	punct	: ,MRE1
R2377	T3652	T3653	nummod	5,CAGCCCAGGCAGGGTGCAGCACGAG
R2378	T3653	T3650	appos	CAGCCCAGGCAGGGTGCAGCACGAG,MRE1
R2379	T3654	T3652	punct	′,5
R2380	T3655	T3653	punct	-,CAGCCCAGGCAGGGTGCAGCACGAG
R2381	T3656	T3653	punct	-,CAGCCCAGGCAGGGTGCAGCACGAG
R2382	T3657	T3653	nummod	3,CAGCCCAGGCAGGGTGCAGCACGAG
R2383	T3658	T3653	punct	′,CAGCCCAGGCAGGGTGCAGCACGAG
R2384	T3659	T3653	punct	;,CAGCCCAGGCAGGGTGCAGCACGAG
R2385	T3660	T3661	nummod	5,CCGCCTCGTGCTGCACCCTGCCTGG
R2386	T3661	T3653	appos	CCGCCTCGTGCTGCACCCTGCCTGG,CAGCCCAGGCAGGGTGCAGCACGAG
R2387	T3662	T3660	punct	′,5
R2388	T3663	T3661	punct	-,CCGCCTCGTGCTGCACCCTGCCTGG
R2389	T3664	T3661	punct	-,CCGCCTCGTGCTGCACCCTGCCTGG
R2390	T3665	T3661	nummod	3,CCGCCTCGTGCTGCACCCTGCCTGG
R2391	T3666	T3661	punct	′,CCGCCTCGTGCTGCACCCTGCCTGG
R2392	T3667	T3497	punct	", ",MRE1
R2393	T3668	T3669	compound	Ndrg1,MRE2
R2394	T3669	T3497	appos	MRE2,MRE1
R2395	T3670	T3669	punct	: ,MRE2
R2396	T3671	T3672	nummod	5,CACACGTTCGCTGCACACGCCGCGG
R2397	T3672	T3669	appos	CACACGTTCGCTGCACACGCCGCGG,MRE2
R2398	T3673	T3671	punct	′,5
R2399	T3674	T3672	punct	-,CACACGTTCGCTGCACACGCCGCGG
R2400	T3675	T3672	punct	-,CACACGTTCGCTGCACACGCCGCGG
R2401	T3676	T3672	nummod	3,CACACGTTCGCTGCACACGCCGCGG
R2402	T3677	T3672	punct	′,CACACGTTCGCTGCACACGCCGCGG
R2403	T3678	T3672	punct	;,CACACGTTCGCTGCACACGCCGCGG
R2404	T3679	T3680	nummod	5,GGGACCGCGGCGTGTGCAGCGAACG
R2405	T3680	T3672	appos	GGGACCGCGGCGTGTGCAGCGAACG,CACACGTTCGCTGCACACGCCGCGG
R2406	T3681	T3679	punct	′,5
R2407	T3682	T3680	punct	-,GGGACCGCGGCGTGTGCAGCGAACG
R2408	T3683	T3680	punct	-,GGGACCGCGGCGTGTGCAGCGAACG
R2409	T3684	T3680	nummod	3,GGGACCGCGGCGTGTGCAGCGAACG
R2410	T3685	T3680	punct	′,GGGACCGCGGCGTGTGCAGCGAACG
R2411	T3686	T3497	punct	", ",MRE1
R2412	T3687	T3688	compound	Ndrg1,MRE3
R2413	T3688	T3497	appos	MRE3,MRE1
R2414	T3689	T3688	punct	",",MRE3
R2415	T3690	T3688	nummod	4,MRE3
R2416	T3691	T3688	punct	: ,MRE3
R2417	T3692	T3693	nummod	5,GGAGTCCTTATGCACACGCGCACGAGCGCGCACGGGCAC
R2418	T3693	T3688	appos	GGAGTCCTTATGCACACGCGCACGAGCGCGCACGGGCAC,MRE3
R2419	T3694	T3692	punct	′,5
R2420	T3695	T3693	punct	-,GGAGTCCTTATGCACACGCGCACGAGCGCGCACGGGCAC
R2421	T3696	T3693	punct	-,GGAGTCCTTATGCACACGCGCACGAGCGCGCACGGGCAC
R2422	T3697	T3693	nummod	3,GGAGTCCTTATGCACACGCGCACGAGCGCGCACGGGCAC
R2423	T3698	T3693	punct	′,GGAGTCCTTATGCACACGCGCACGAGCGCGCACGGGCAC
R2424	T3699	T3693	punct	;,GGAGTCCTTATGCACACGCGCACGAGCGCGCACGGGCAC
R2425	T3700	T3701	nummod	5,TGGTGTGCCCGTGCGCGCTCGTGCGCGTGTGCATAAGGAC
R2426	T3701	T3693	appos	TGGTGTGCCCGTGCGCGCTCGTGCGCGTGTGCATAAGGAC,GGAGTCCTTATGCACACGCGCACGAGCGCGCACGGGCAC
R2427	T3702	T3700	punct	′,5
R2428	T3703	T3701	punct	-,TGGTGTGCCCGTGCGCGCTCGTGCGCGTGTGCATAAGGAC
R2429	T3704	T3701	punct	-,TGGTGTGCCCGTGCGCGCTCGTGCGCGTGTGCATAAGGAC
R2430	T3705	T3701	nummod	3,TGGTGTGCCCGTGCGCGCTCGTGCGCGTGTGCATAAGGAC
R2431	T3706	T3701	punct	′,TGGTGTGCCCGTGCGCGCTCGTGCGCGTGTGCATAAGGAC
R2432	T3707	T3497	punct	", ",MRE1
R2433	T3708	T3709	compound	Sepw1,MRE1
R2434	T3709	T3497	appos	MRE1,MRE1
R2435	T3710	T3709	punct	: ,MRE1
R2436	T3711	T3712	nummod	5,GAGGCAGTCGGCTGTGCGCACGGCCCCACGCTC
R2437	T3712	T3709	appos	GAGGCAGTCGGCTGTGCGCACGGCCCCACGCTC,MRE1
R2438	T3713	T3711	punct	′,5
R2439	T3714	T3712	punct	-,GAGGCAGTCGGCTGTGCGCACGGCCCCACGCTC
R2440	T3715	T3712	punct	-,GAGGCAGTCGGCTGTGCGCACGGCCCCACGCTC
R2441	T3716	T3712	nummod	3,GAGGCAGTCGGCTGTGCGCACGGCCCCACGCTC
R2442	T3717	T3712	punct	′,GAGGCAGTCGGCTGTGCGCACGGCCCCACGCTC
R2443	T3718	T3712	punct	;,GAGGCAGTCGGCTGTGCGCACGGCCCCACGCTC
R2444	T3719	T3720	nummod	5,CTCTGAGCGTGGGGCCGTGCGCACAGCCGACTGC
R2445	T3720	T3712	appos	CTCTGAGCGTGGGGCCGTGCGCACAGCCGACTGC,GAGGCAGTCGGCTGTGCGCACGGCCCCACGCTC
R2446	T3721	T3719	punct	′,5
R2447	T3722	T3720	punct	-,CTCTGAGCGTGGGGCCGTGCGCACAGCCGACTGC
R2448	T3723	T3720	punct	-,CTCTGAGCGTGGGGCCGTGCGCACAGCCGACTGC
R2449	T3724	T3720	nummod	3,CTCTGAGCGTGGGGCCGTGCGCACAGCCGACTGC
R2450	T3725	T3720	punct	′,CTCTGAGCGTGGGGCCGTGCGCACAGCCGACTGC
R2451	T3726	T3497	punct	", ",MRE1
R2452	T3727	T3728	compound	Sepw1,MRE2
R2453	T3728	T3497	appos	MRE2,MRE1
R2454	T3729	T3728	punct	: ,MRE2
R2455	T3730	T3731	nummod	5,ATGGTTTTGGGGGTGCGCAGGGGGTCTG
R2456	T3731	T3728	appos	ATGGTTTTGGGGGTGCGCAGGGGGTCTG,MRE2
R2457	T3732	T3730	punct	′,5
R2458	T3733	T3731	punct	-,ATGGTTTTGGGGGTGCGCAGGGGGTCTG
R2459	T3734	T3731	punct	-,ATGGTTTTGGGGGTGCGCAGGGGGTCTG
R2460	T3735	T3731	nummod	3,ATGGTTTTGGGGGTGCGCAGGGGGTCTG
R2461	T3736	T3731	punct	′,ATGGTTTTGGGGGTGCGCAGGGGGTCTG
R2462	T3737	T3731	punct	;,ATGGTTTTGGGGGTGCGCAGGGGGTCTG
R2463	T3738	T3739	nummod	5,CGACAGACCCCCTGCGCACCCCCAAAAC
R2464	T3739	T3731	appos	CGACAGACCCCCTGCGCACCCCCAAAAC,ATGGTTTTGGGGGTGCGCAGGGGGTCTG
R2465	T3740	T3738	punct	′,5
R2466	T3741	T3739	punct	-,CGACAGACCCCCTGCGCACCCCCAAAAC
R2467	T3742	T3739	punct	-,CGACAGACCCCCTGCGCACCCCCAAAAC
R2468	T3743	T3739	nummod	3,CGACAGACCCCCTGCGCACCCCCAAAAC
R2469	T3744	T3739	punct	′,CGACAGACCCCCTGCGCACCCCCAAAAC
R2470	T3745	T3497	punct	", ",MRE1
R2471	T3746	T3747	compound	Slc39a10,MRE1
R2472	T3747	T3497	appos	MRE1,MRE1
R2473	T3748	T3747	punct	: ,MRE1
R2474	T3749	T3750	nummod	5,GAATACACGACTGGGTGCAGCCGGGGTTTGG
R2475	T3750	T3747	appos	GAATACACGACTGGGTGCAGCCGGGGTTTGG,MRE1
R2476	T3751	T3749	punct	′,5
R2477	T3752	T3750	punct	-,GAATACACGACTGGGTGCAGCCGGGGTTTGG
R2478	T3753	T3750	punct	-,GAATACACGACTGGGTGCAGCCGGGGTTTGG
R2479	T3754	T3750	nummod	3,GAATACACGACTGGGTGCAGCCGGGGTTTGG
R2480	T3755	T3750	punct	′,GAATACACGACTGGGTGCAGCCGGGGTTTGG
R2481	T3756	T3750	punct	;,GAATACACGACTGGGTGCAGCCGGGGTTTGG
R2482	T3757	T3758	nummod	5,GGTACCAAACCCCGGCTGCACCCAGTCGTGTA
R2483	T3758	T3750	appos	GGTACCAAACCCCGGCTGCACCCAGTCGTGTA,GAATACACGACTGGGTGCAGCCGGGGTTTGG
R2484	T3759	T3757	punct	′,5
R2485	T3760	T3758	punct	-,GGTACCAAACCCCGGCTGCACCCAGTCGTGTA
R2486	T3761	T3758	punct	-,GGTACCAAACCCCGGCTGCACCCAGTCGTGTA
R2487	T3762	T3758	nummod	3,GGTACCAAACCCCGGCTGCACCCAGTCGTGTA
R2488	T3763	T3758	punct	′,GGTACCAAACCCCGGCTGCACCCAGTCGTGTA
R2489	T3764	T3497	punct	", ",MRE1
R2490	T3765	T3766	compound	Slc39a10,MRE2
R2491	T3766	T3497	appos	MRE2,MRE1
R2492	T3767	T3766	punct	: ,MRE2
R2493	T3768	T3769	nummod	5,GCGGAGAGGAGATGCACACGGCACTCG
R2494	T3769	T3766	appos	GCGGAGAGGAGATGCACACGGCACTCG,MRE2
R2495	T3770	T3768	punct	′,5
R2496	T3771	T3769	punct	-,GCGGAGAGGAGATGCACACGGCACTCG
R2497	T3772	T3769	punct	-,GCGGAGAGGAGATGCACACGGCACTCG
R2498	T3773	T3769	nummod	3,GCGGAGAGGAGATGCACACGGCACTCG
R2499	T3774	T3769	punct	′,GCGGAGAGGAGATGCACACGGCACTCG
R2500	T3775	T3769	punct	;,GCGGAGAGGAGATGCACACGGCACTCG
R2501	T3776	T3777	nummod	5,CACTCGAGTGCCGTGTGCATCTCCTCT
R2502	T3777	T3769	appos	CACTCGAGTGCCGTGTGCATCTCCTCT,GCGGAGAGGAGATGCACACGGCACTCG
R2503	T3778	T3776	punct	′,5
R2504	T3779	T3777	punct	-,CACTCGAGTGCCGTGTGCATCTCCTCT
R2505	T3780	T3777	punct	-,CACTCGAGTGCCGTGTGCATCTCCTCT
R2506	T3781	T3777	nummod	3,CACTCGAGTGCCGTGTGCATCTCCTCT
R2507	T3782	T3777	punct	′,CACTCGAGTGCCGTGTGCATCTCCTCT
R2508	T3783	T3497	punct	", ",MRE1
R2509	T3784	T3785	nmod	Specificity,protein
R2510	T3785	T3786	nmod	protein,sequence
R2511	T3786	T3497	appos	sequence,MRE1
R2512	T3787	T3785	nummod	1,protein
R2513	T3788	T3785	punct	(,protein
R2514	T3789	T3785	appos	Sp1,protein
R2515	T3790	T3786	punct	),sequence
R2516	T3791	T3786	compound	binding,sequence
R2517	T3792	T3793	punct	(,10
R2518	T3793	T3786	parataxis	10,sequence
R2519	T3794	T3793	punct	),10
R2520	T3795	T3497	punct	: ,MRE1
R2521	T3796	T3797	nummod	5,CGAGGCCCCGCCCAG
R2522	T3797	T3497	appos	CGAGGCCCCGCCCAG,MRE1
R2523	T3798	T3796	punct	′,5
R2524	T3799	T3797	punct	-,CGAGGCCCCGCCCAG
R2525	T3800	T3797	punct	-,CGAGGCCCCGCCCAG
R2526	T3801	T3797	nummod	3,CGAGGCCCCGCCCAG
R2527	T3802	T3797	punct	′,CGAGGCCCCGCCCAG
R2528	T3803	T3797	punct	;,CGAGGCCCCGCCCAG
R2529	T3804	T3805	nummod	5,TCGACTGGGCGGGGCCTCGAGct
R2530	T3805	T3797	appos	TCGACTGGGCGGGGCCTCGAGct,CGAGGCCCCGCCCAG
R2531	T3806	T3804	punct	′,5
R2532	T3807	T3805	punct	-,TCGACTGGGCGGGGCCTCGAGct
R2533	T3808	T3805	punct	-,TCGACTGGGCGGGGCCTCGAGct
R2534	T3809	T3805	nummod	3,TCGACTGGGCGGGGCCTCGAGct
R2535	T3810	T3805	punct	′,TCGACTGGGCGGGGCCTCGAGct
R2536	T3811	T3488	punct	.,annealed
R2537	T3873	T3888	compound	Mtf1loxP,embryo
R2538	T3874	T3898	conj	streptomycin,serum
R2539	T3875	T3876	compound	Cell,culture
R2540	T3878	T3879	amod	Primary,fibroblasts
R2541	T3879	T3881	nsubjpass	fibroblasts,isolated
R2542	T3880	T3879	amod	embryonic,fibroblasts
R2543	T3882	T3881	auxpass	were,isolated
R2544	T3883	T3881	prep	from,isolated
R2545	T3884	T3885	det	a,day
R2546	T3885	T3887	npadvmod	day,old
R2547	T3886	T3885	nummod	12.5,day
R2548	T3887	T3888	amod	old,embryo
R2549	T3888	T3883	pobj	embryo,from
R2550	T3889	T3888	compound	mouse,embryo
R2551	T3890	T3881	cc	and,isolated
R2552	T3891	T3881	conj	grown,isolated
R2553	T3892	T3891	prep	in,grown
R2554	T3893	T3892	pobj	DMEM,in
R2555	T3894	T3893	acl	supplemented,DMEM
R2556	T3895	T3894	prep	with,supplemented
R2557	T3896	T3897	nummod	10,%
R2558	T3897	T3898	nmod	%,serum
R2559	T3898	T3895	pobj	serum,with
R2560	T3899	T3898	amod	fetal,serum
R2561	T3900	T3898	amod	bovine,serum
R2562	T3901	T3902	punct	(,ICN
R2563	T3902	T3898	parataxis	ICN,serum
R2564	T3903	T3902	punct	),ICN
R2565	T3904	T3898	punct	", ",serum
R2566	T3905	T3906	nummod	100,U
R2567	T3906	T3874	nmod	U,streptomycin
R2568	T3907	T3908	punct	/,ml
R2569	T3908	T3906	prep	ml,U
R2570	T3909	T3874	compound	penicillin,streptomycin
R2571	T3910	T3874	punct	–,streptomycin
R2572	T3911	T3912	punct	(,BRL
R2573	T3912	T3874	parataxis	BRL,streptomycin
R2574	T3913	T3912	compound	Gibco,BRL
R2575	T3914	T3912	punct	),BRL
R2576	T3915	T3874	cc	and,streptomycin
R2577	T3916	T3917	nummod	2,mM
R2578	T3917	T3918	compound	mM,glutamine
R2579	T3918	T3874	conj	glutamine,streptomycin
R2580	T3919	T3918	compound	l,glutamine
R2581	T3920	T3918	punct	-,glutamine
R2582	T3921	T3922	punct	(,BRL
R2583	T3922	T3918	parataxis	BRL,glutamine
R2584	T3923	T3922	compound	Gibco,BRL
R2585	T3924	T3922	punct	),BRL
R2586	T3925	T3881	punct	.,isolated
R2587	T3927	T3928	nummod	100,mm
R2588	T3928	T3929	compound	mm,plates
R2589	T3929	T3930	nsubjpass	plates,transfected
R2590	T3931	T3929	prep	with,plates
R2591	T3932	T3933	amod	primary,cells
R2592	T3933	T3931	pobj	cells,with
R2593	T3934	T3930	auxpass	were,transfected
R2594	T3935	T3930	prep	with,transfected
R2595	T3936	T3937	nummod	10,µg
R2596	T3937	T3935	pobj	µg,with
R2597	T3938	T3937	prep	of,µg
R2598	T3939	T3940	det	an,plasmid
R2599	T3940	T3938	pobj	plasmid,of
R2600	T3941	T3940	compound	expression,plasmid
R2601	T3942	T3940	acl	coding,plasmid
R2602	T3943	T3942	prep	for,coding
R2603	T3944	T3945	amod	simian,virus
R2604	T3945	T3946	nmod	virus,antigen
R2605	T3946	T3943	pobj	antigen,for
R2606	T3947	T3945	nummod	40,virus
R2607	T3948	T3945	punct	(,virus
R2608	T3949	T3945	appos	SV40,virus
R2609	T3950	T3946	punct	),antigen
R2610	T3951	T3952	amod	large,T
R2611	T3952	T3946	compound	T,antigen
R2612	T3953	T3946	acl	driven,antigen
R2613	T3954	T3953	agent	by,driven
R2614	T3955	T3956	det	the,promoter
R2615	T3956	T3954	pobj	promoter,by
R2616	T3957	T3956	nmod	cytomegalovirus,promoter
R2617	T3958	T3957	punct	(,cytomegalovirus
R2618	T3959	T3957	appos	CMV,cytomegalovirus
R2619	T3960	T3956	punct	),promoter
R2620	T3961	T3930	punct	", ",transfected
R2621	T3962	T3930	advcl	using,transfected
R2622	T3963	T3964	nmod	lipofectamine,reagent
R2623	T3964	T3962	dobj	reagent,using
R2624	T3965	T3963	punct	™,lipofectamine
R2625	T3966	T3967	punct	(,Invitrogen
R2626	T3967	T3964	parataxis	Invitrogen,reagent
R2627	T3968	T3967	punct	),Invitrogen
R2628	T3969	T3962	prep	according,using
R2629	T3970	T3969	prep	to,according
R2630	T3971	T3972	det	the,manufacturer
R2631	T3972	T3973	poss	manufacturer,instructions
R2632	T3973	T3970	pobj	instructions,to
R2633	T3974	T3972	case	's,manufacturer
R2634	T3975	T3930	punct	.,transfected
R2635	T3977	T3978	compound	Cell,foci
R2636	T3978	T3979	nsubjpass	foci,isolated
R2637	T3980	T3979	auxpass	were,isolated
R2638	T3981	T3979	cc	and,isolated
R2639	T3982	T3983	det	the,line
R2640	T3983	T3989	nsubjpass	line,derived
R2641	T3984	T3983	amod	immortalized,line
R2642	T3985	T3986	nmod	mouse,fibroblast
R2643	T3986	T3983	compound	fibroblast,line
R2644	T3987	T3986	amod	embryonic,fibroblast
R2645	T3988	T3983	compound	cell,line
R2646	T3989	T3979	conj	derived,isolated
R2647	T3990	T3983	appos	ckoC,line
R2648	T3991	T3989	auxpass	was,derived
R2649	T3992	T3989	prep	from,derived
R2650	T3993	T3992	pobj	one,from
R2651	T3994	T3993	prep	of,one
R2652	T3995	T3994	pobj	them,of
R2653	T3996	T3989	punct	.,derived
R2654	T3998	T3999	det	The,genotype
R2655	T3999	T4001	nsubjpass	genotype,confirmed
R2656	T4000	T3999	compound	Mtf1loxP,genotype
R2657	T4002	T3999	prep	of,genotype
R2658	T4003	T4004	det	this,line
R2659	T4004	T4002	pobj	line,of
R2660	T4005	T4006	advmod	as,as
R2661	T4006	T3999	cc	as,genotype
R2662	T4007	T4006	advmod	well,as
R2663	T4008	T4009	det	the,integration
R2664	T4009	T3999	conj	integration,genotype
R2665	T4010	T4009	amod	genomic,integration
R2666	T4011	T4009	prep	of,integration
R2667	T4012	T4013	det	the,antigen
R2668	T4013	T4011	pobj	antigen,of
R2669	T4014	T4013	compound	T,antigen
R2670	T4015	T4001	auxpass	were,confirmed
R2671	T4016	T4001	agent	by,confirmed
R2672	T4017	T4016	pobj	PCR,by
R2673	T4018	T4001	punct	.,confirmed
R2674	T4020	T4021	nummod	100,mm
R2675	T4021	T4022	compound	mm,plates
R2676	T4022	T4023	nsubjpass	plates,transfected
R2677	T4024	T4022	prep	with,plates
R2678	T4025	T4026	det	these,cells
R2679	T4026	T4024	pobj	cells,with
R2680	T4027	T4023	auxpass	were,transfected
R2681	T4028	T4023	advmod	further,transfected
R2682	T4029	T4023	agent	by,transfected
R2683	T4030	T4031	det	the,method
R2684	T4031	T4029	pobj	method,by
R2685	T4032	T4033	compound	calcium,phosphate
R2686	T4033	T4031	compound	phosphate,method
R2687	T4034	T4035	punct	(,30
R2688	T4035	T4031	parataxis	30,method
R2689	T4036	T4035	punct	),30
R2690	T4037	T4031	prep	with,method
R2691	T4038	T4039	nummod	19.6,µg
R2692	T4039	T4037	pobj	µg,with
R2693	T4040	T4039	prep	of,µg
R2694	T4041	T4042	det	an,plasmid
R2695	T4042	T4040	pobj	plasmid,of
R2696	T4043	T4042	compound	expression,plasmid
R2697	T4044	T4042	prep	for,plasmid
R2698	T4045	T4046	compound	Cre,recombinase
R2699	T4046	T4044	pobj	recombinase,for
R2700	T4047	T4042	acl	driven,plasmid
R2701	T4048	T4047	agent	by,driven
R2702	T4049	T4050	det	the,promoter
R2703	T4050	T4048	pobj	promoter,by
R2704	T4051	T4050	compound	CMV,promoter
R2705	T4052	T4039	cc	and,µg
R2706	T4053	T4054	nummod	0.4,µg
R2707	T4054	T4039	conj	µg,µg
R2708	T4055	T4054	prep	of,µg
R2709	T4056	T4057	det	an,plasmid
R2710	T4057	T4055	pobj	plasmid,of
R2711	T4058	T4057	compound	expression,plasmid
R2712	T4059	T4057	prep	for,plasmid
R2713	T4060	T4061	det	the,gene
R2714	T4061	T4059	pobj	gene,for
R2715	T4062	T4061	compound	neomycin,gene
R2716	T4063	T4061	compound	resistance,gene
R2717	T4064	T4061	prep	under,gene
R2718	T4065	T4066	det	the,control
R2719	T4066	T4064	pobj	control,under
R2720	T4067	T4066	prep	of,control
R2721	T4068	T4069	det	the,promoter
R2722	T4069	T4067	pobj	promoter,of
R2723	T4070	T4069	compound	TK,promoter
R2724	T4071	T4023	punct	.,transfected
R2725	T4073	T4074	advmod	Stably,transfected
R2726	T4074	T4075	amod	transfected,cells
R2727	T4075	T4076	nsubjpass	cells,selected
R2728	T4077	T4076	auxpass	were,selected
R2729	T4078	T4076	prep	in,selected
R2730	T4079	T4080	det	the,presence
R2731	T4080	T4078	pobj	presence,in
R2732	T4081	T4080	prep	of,presence
R2733	T4082	T4083	nummod	0.4,µg
R2734	T4083	T4084	nmod	µg,G418
R2735	T4084	T4081	pobj	G418,of
R2736	T4085	T4086	punct	/,µl
R2737	T4086	T4083	prep	µl,µg
R2738	T4087	T4088	punct	(,Calbiochem
R2739	T4088	T4084	parataxis	Calbiochem,G418
R2740	T4089	T4088	punct	),Calbiochem
R2741	T4090	T4076	punct	", ",selected
R2742	T4091	T4092	amod	isolated,clones
R2743	T4092	T4093	nsubjpass	clones,harvested
R2744	T4093	T4076	conj	harvested,selected
R2745	T4094	T4092	prep	of,clones
R2746	T4095	T4096	amod	resistant,cells
R2747	T4096	T4094	pobj	cells,of
R2748	T4097	T4093	auxpass	were,harvested
R2749	T4098	T4093	cc	and,harvested
R2750	T4099	T4093	conj	grown,harvested
R2751	T4100	T4099	advmod	independently,grown
R2752	T4101	T4093	punct	", ",harvested
R2753	T4102	T4093	cc	and,harvested
R2754	T4103	T4104	det	the,expression
R2755	T4104	T4105	nsubjpass	expression,analyzed
R2756	T4105	T4093	conj	analyzed,harvested
R2757	T4106	T4104	prep	of,expression
R2758	T4107	T4108	compound	Cre,recombinase
R2759	T4108	T4106	pobj	recombinase,of
R2760	T4109	T4104	cc	and,expression
R2761	T4110	T4104	conj	excision,expression
R2762	T4111	T4110	prep	of,excision
R2763	T4112	T4113	nmod	exons,3
R2764	T4113	T4111	pobj	3,of
R2765	T4114	T4113	cc	and,3
R2766	T4115	T4113	conj	4,3
R2767	T4116	T4113	prep	of,3
R2768	T4117	T4116	pobj	Mtf1,of
R2769	T4118	T4105	auxpass	were,analyzed
R2770	T4119	T4105	agent	by,analyzed
R2771	T4120	T4121	compound	RT,PCR
R2772	T4121	T4119	pobj	PCR,by
R2773	T4122	T4121	punct	–,PCR
R2774	T4123	T4105	punct	.,analyzed
R2775	T4125	T4126	det	The,lines
R2776	T4126	T4128	nsubjpass	lines,chosen
R2777	T4127	T4126	compound	cell,lines
R2778	T4129	T4126	appos	delC19,lines
R2779	T4130	T4129	punct	", ",delC19
R2780	T4131	T4129	conj	delC21,delC19
R2781	T4132	T4131	cc	and,delC21
R2782	T4133	T4131	conj	delC23,delC21
R2783	T4134	T4126	prep	with,lines
R2784	T4135	T4136	det	a,deletion
R2785	T4136	T4134	pobj	deletion,with
R2786	T4137	T4136	prep	of,deletion
R2787	T4138	T4137	pobj	Mtf1,of
R2788	T4139	T4128	auxpass	were,chosen
R2789	T4140	T4128	prep	for,chosen
R2790	T4141	T4142	amod	further,experiments
R2791	T4142	T4140	pobj	experiments,for
R2792	T4143	T4128	punct	.,chosen
R2795	T4279	T4280	compound	Cytotoxicity,assay
R2796	T4282	T4283	nsubjpass	Samples,plated
R2797	T4284	T4282	prep	of,Samples
R2798	T4285	T4286	quantmod	1,104
R2799	T4286	T4288	nummod	104,cells
R2800	T4287	T4286	punct	×,104
R2801	T4288	T4284	pobj	cells,of
R2802	T4289	T4290	punct	/,well
R2803	T4290	T4288	prep	well,cells
R2804	T4291	T4283	auxpass	were,plated
R2805	T4292	T4283	prep	in,plated
R2806	T4293	T4294	nummod	96,well
R2807	T4294	T4296	compound	well,plates
R2808	T4295	T4294	punct	-,well
R2809	T4296	T4292	pobj	plates,in
R2810	T4297	T4298	compound	tissue,culture
R2811	T4298	T4296	compound	culture,plates
R2812	T4299	T4283	cc	and,plated
R2813	T4300	T4283	conj	allowed,plated
R2814	T4301	T4302	aux	to,adhere
R2815	T4302	T4300	xcomp	adhere,allowed
R2816	T4303	T4302	prep	for,adhere
R2817	T4304	T4305	nummod	24,h
R2818	T4305	T4303	pobj	h,for
R2819	T4306	T4283	punct	.,plated
R2820	T4308	T4309	det	The,cells
R2821	T4309	T4310	nsubjpass	cells,pre-incubated
R2822	T4311	T4310	auxpass	were,pre-incubated
R2823	T4312	T4310	advmod	then,pre-incubated
R2824	T4313	T4310	prep	for,pre-incubated
R2825	T4314	T4315	nummod	24,h
R2826	T4315	T4313	pobj	h,for
R2827	T4316	T4310	prep	in,pre-incubated
R2828	T4317	T4316	pobj	medium,in
R2829	T4318	T4317	acl	containing,medium
R2830	T4319	T4320	nummod	0,µM
R2831	T4320	T4318	dobj	µM,containing
R2832	T4321	T4319	punct	", ",0
R2833	T4322	T4319	conj	5,0
R2834	T4323	T4322	punct	", ",5
R2835	T4324	T4322	conj	10,5
R2836	T4325	T4324	punct	", ",10
R2837	T4326	T4324	conj	25,10
R2838	T4327	T4326	cc	or,25
R2839	T4328	T4326	conj	50,25
R2840	T4329	T4330	nmod	l,sulfoximine
R2841	T4330	T4320	appos	sulfoximine,µM
R2842	T4331	T4330	punct	-,sulfoximine
R2843	T4332	T4330	nmod	buthionine,sulfoximine
R2844	T4333	T4330	punct	-,sulfoximine
R2845	T4334	T4330	punct	[,sulfoximine
R2846	T4335	T4330	nmod	S,sulfoximine
R2847	T4336	T4330	punct	",",sulfoximine
R2848	T4337	T4330	nmod	R,sulfoximine
R2849	T4338	T4330	punct	],sulfoximine
R2850	T4339	T4330	punct	-,sulfoximine
R2851	T4340	T4330	punct	(,sulfoximine
R2852	T4341	T4330	appos	BSO,sulfoximine
R2853	T4342	T4320	punct	),µM
R2854	T4343	T4344	punct	(,Sigma
R2855	T4344	T4320	parataxis	Sigma,µM
R2856	T4345	T4344	punct	),Sigma
R2857	T4346	T4320	punct	", ",µM
R2858	T4347	T4348	det	a,drug
R2859	T4348	T4320	appos	drug,µM
R2860	T4349	T4350	dep	that,inhibits
R2861	T4350	T4348	relcl	inhibits,drug
R2862	T4351	T4352	compound	glutathione,synthesis
R2863	T4352	T4350	dobj	synthesis,inhibits
R2864	T4353	T4354	punct	(,31
R2865	T4354	T4350	parataxis	31,inhibits
R2866	T4355	T4354	punct	),31
R2867	T4356	T4310	punct	.,pre-incubated
R2868	T4358	T4359	advmod	Later,exposed
R2869	T4360	T4359	punct	", ",exposed
R2870	T4361	T4359	nsubjpass	cells,exposed
R2871	T4362	T4359	auxpass	were,exposed
R2872	T4363	T4359	prep	to,exposed
R2873	T4364	T4365	nummod	0,µM
R2874	T4365	T4372	compound	µM,CdCl2
R2875	T4366	T4364	punct	", ",0
R2876	T4367	T4364	conj	5,0
R2877	T4368	T4367	punct	", ",5
R2878	T4369	T4367	conj	10,5
R2879	T4370	T4369	cc	or,10
R2880	T4371	T4369	conj	20,10
R2881	T4372	T4363	pobj	CdCl2,to
R2882	T4373	T4359	prep	in,exposed
R2883	T4374	T4375	det	the,medium
R2884	T4375	T4373	pobj	medium,in
R2885	T4376	T4375	amod	specified,medium
R2886	T4377	T4375	compound	pre-incubation,medium
R2887	T4378	T4359	prep	for,exposed
R2888	T4379	T4380	det	an,h
R2889	T4380	T4378	pobj	h,for
R2890	T4381	T4380	amod	additional,h
R2891	T4382	T4380	nummod	24,h
R2892	T4383	T4359	punct	.,exposed
R2893	T4385	T4386	nsubjpass	Cytotoxicity,determined
R2894	T4387	T4386	auxpass	was,determined
R2895	T4388	T4386	agent	by,determined
R2896	T4389	T4390	det	the,Kit
R2897	T4390	T4388	pobj	Kit,by
R2898	T4391	T4392	npadvmod	MTT,based
R2899	T4392	T4390	amod	based,Kit
R2900	T4393	T4394	punct	(,bromid
R2901	T4394	T4391	parataxis	bromid,MTT
R2902	T4395	T4396	nummod	3,dimethylthiazol
R2903	T4396	T4394	nmod	dimethylthiazol,bromid
R2904	T4397	T4396	punct	-,dimethylthiazol
R2905	T4398	T4396	punct	[,dimethylthiazol
R2906	T4399	T4396	nummod	4,dimethylthiazol
R2907	T4400	T4396	punct	",",dimethylthiazol
R2908	T4401	T4396	nummod	5,dimethylthiazol
R2909	T4402	T4396	punct	-,dimethylthiazol
R2910	T4403	T4396	punct	-,dimethylthiazol
R2911	T4404	T4405	nummod	2,yl
R2912	T4405	T4396	appos	yl,dimethylthiazol
R2913	T4406	T4405	punct	-,yl
R2914	T4407	T4396	punct	],dimethylthiazol
R2915	T4408	T4396	punct	-,dimethylthiazol
R2916	T4409	T4396	nummod	2,dimethylthiazol
R2917	T4410	T4394	punct	",",bromid
R2918	T4411	T4412	nummod	5,diphenyl
R2919	T4412	T4394	compound	diphenyl,bromid
R2920	T4413	T4412	punct	-,diphenyl
R2921	T4414	T4394	compound	tetrazolium,bromid
R2922	T4415	T4394	punct	),bromid
R2923	T4416	T4392	punct	-,based
R2924	T4417	T4390	compound	Cell,Kit
R2925	T4418	T4390	compound	Proliferation,Kit
R2926	T4419	T4390	nummod	I,Kit
R2927	T4420	T4421	punct	(,Roche
R2928	T4421	T4390	parataxis	Roche,Kit
R2929	T4422	T4421	punct	),Roche
R2930	T4423	T4386	prep	according,determined
R2931	T4424	T4423	prep	to,according
R2932	T4425	T4426	det	the,manufacturer
R2933	T4426	T4427	poss	manufacturer,instructions
R2934	T4427	T4424	pobj	instructions,to
R2935	T4428	T4426	case	's,manufacturer
R2936	T4429	T4386	punct	.,determined
R1329	T2196	T2190	appos	cre,transgene
R1470	T2341	T2322	conj	H2O,CdSO4
R1577	T2628	T2626	pobj	Kit,with
R1721	T2786	T2782	dobj	genes,obtain
R2161	T3430	T3425	dobj	oligonucleotides,incubating

2_test

Id	Subject	Object	Predicate	Lexical cue
16221973-2440339-77403413	2582-2584	2440339	denotes	27
16221973-12519945-77403414	4276-4278	12519945	denotes	28
16221973-390497-77403415	5093-5095	390497	denotes	29
16221973-8467794-77403416	5818-5820	8467794	denotes	10
16221973-8467794-77403417	6699-6701	8467794	denotes	10
16221973-8467794-77403418	6832-6834	8467794	denotes	10
16221973-8467794-77403419	7593-7595	8467794	denotes	10
16221973-4705382-77403420	8483-8485	4705382	denotes	30
16221973-38242-77403421	9347-9349	38242	denotes	31

craft-ca-core-dev

Below, discontinuous spans are shown in the bag model. You can change it to the chain model.

Id	Subject	Object	Predicate	Lexical cue
T1969	37-41	PR:000010717	denotes	Mtf1
T1970	63-67	NCBITaxon:10088	denotes	mice
T1971	72-77	UBERON:0002107	denotes	liver
T1972	96-100	PR:000010717	denotes	Mtf1
T1973	122-126	NCBITaxon:10088	denotes	mice
T1974	205-219	SO:0000040	denotes	genomic clones
T1975	231-236	SO:0000147	denotes	exons
T1976	247-251	PR:000010717	denotes	Mtf1
T1977	277-281	SO:0000704	denotes	gene
T1978	282-298	SO:0001644	denotes	targeting vector
T1979	352-360	CHEBI:7507	denotes	neomycin
T1980	372-380	SO:0005853	denotes	cassette
T1981	391-398	SO:0000357	denotes	flanked
T1982	406-416	SO:0000346	denotes	loxP sites
T1983	453-457	SO:0000147	denotes	exon
T1984	463-467	PR:000010717	denotes	Mtf1
T1985	479-488	SO:0000346	denotes	loxP site
T1986	525-529	SO:0000147	denotes	exon
T1987	557-565	SO:0005853	denotes	cassette
T1988	602-606	SO:0000147	denotes	exon
T1989	614-622	CL:0002322	denotes	ES cells
T1990	663-679	SO:0001644	denotes	targeting vector
T1991	709-713	CHEBI:42768	denotes	G418
T1992	813-823	GO:0010467	denotes	expression
T1993	873-881	SO:0005853	denotes	cassette
T1994	887-891	NCBITaxon:10088	denotes	mice
T1995	914-918	PR:000010717	denotes	Mtf1
T1996	918-922	SO:0000346	denotes	loxP
T1997	923-929	SO:0001023	denotes	allele
T1998	990-1001	UBERON:0000358	denotes	blastocysts
T1999	1058-1065	NCBITaxon:33208	denotes	animals
T2000	1067-1071	PR:000010717	denotes	Mtf1
T2001	1071-1075	SO:0000346	denotes	loxP
T2002	1076-1080	SO:0000346	denotes	loxP
T2003	1136-1138	PR:000010788	denotes	Mx
T2004	1200-1205	UBERON:0002107	denotes	liver
T2005	1215-1219	PR:000010717	denotes	Mtf1
T2006	1239-1243	NCBITaxon:10088	denotes	mice
T2007	1286-1293	SO:0000112	denotes	primers
T2008	1390-1394	PR:000010717	denotes	Mtf1
T2009	1394-1398	SO:0000346	denotes	loxP
T2010	1412-1418	SO:0001023	denotes	allele
T2371	1670-1674	SO:0000346	denotes	loxP
T2372	1675-1679	SO:0000346	denotes	loxP
T2373	1688-1692	PR:000010717	denotes	Mtf1
T2374	1692-1696	SO:0000346	denotes	loxP
T2375	1717-1727	UBERON:0001179	denotes	peritoneal
T2376	1764-1779	SO:0000985	denotes	double-stranded
T2378	1784-1796,1811-1815	CHEBI:76777	denotes	polyinosinic acid
T2379	1817-1819	CHEBI:76777	denotes	pI
T2380	1969-1974	PR:000010717	denotes	MTF-1
T2381	1982-1986	SO:0000704	denotes	gene
T2382	2011-2015	NCBITaxon:10088	denotes	mice
T2383	2028-2030	CHEBI:76777	denotes	pI
T2384	2084-2088	NCBITaxon:10088	denotes	mice
T2385	2120-2122	CHEBI:76777	denotes	pI
T2386	2201-2206	CHEBI:50292	denotes	CdSO4
T2387	2213-2218	CHEBI:50292	denotes	CdSO4
T2388	2222-2225	CHEBI:15377	denotes	H2O
T2389	2270-2273	CHEBI:15377	denotes	H2O
T2458	2389-2394	UBERON:0002107	denotes	liver
T2459	2395-2401	UBERON:0000479	denotes	tissue
T2460	2405-2407	CHEBI:76777	denotes	pI
T2461	2445-2449	PR:000010717	denotes	Mtf1
T2462	2449-2451	PR:000010788	denotes	Mx
T2463	2460-2464	PR:000010717	denotes	Mtf1
T2464	2464-2468	SO:0000346	denotes	loxP
T2465	2469-2473	NCBITaxon:10088	denotes	mice
T2466	2587-2591	SO:0000704	denotes	Gene
T2467	2587-2602	GO:0010467	denotes	Gene expression
T2468	2683-2688	NCBITaxon:10088	denotes	Mouse
T2469	2689-2695	SO:0001026	denotes	Genome
T2470	2787-2795	CHEBI:33893	denotes	reagents
T2471	2851-2866	SO:0000985	denotes	Double-Stranded
T2472	2991-3001	SO:0000673	denotes	Transcript
T2473	3619-3624	PR:000010717	denotes	MTF-1
T2474	3637-3642	SO:0000704	denotes	genes
T2475	3644-3649	SO:0000704	denotes	Genes
T2476	3687-3696	GO:0010467	denotes	expressed
T2477	3742-3752	GO:0010467	denotes	expression
T2478	3904-3908	SO:0000704	denotes	gene
T2479	3948-3952	NCBITaxon:10088	denotes	mice
T2480	3990-3994	NCBITaxon:10088	denotes	mice
T2481	4061-4070	SO:0000993	denotes	consensus
T2482	4095-4103	SO:0000167	denotes	promoter
T2483	4153-4158	SO:0000704	denotes	genes
T2484	4225-4231	SO:0001026	denotes	Genome
T3275	5585-5590	UBERON:0002107	denotes	liver
T3276	5591-5597	UBERON:0000479	denotes	tissue
T3277	5610-5616	UBERON:0000479	denotes	Tissue
T3278	5636-5643	CHEBI:33893	denotes	Reagent
T3279	5721-5726	NCBITaxon:10088	denotes	mouse
T3280	5727-5733	UBERON:0000479	denotes	tissue
T3281	5748-5755	CHEBI:33893	denotes	reagent
T3282	5885-5888	CHEBI:37972	denotes	32P
T3283	5901-5933	SO:0000442	denotes	double-stranded oligonucleotides
T3284	5953-5958	UBERON:0002107	denotes	liver
T3285	6226-6234	GO:0097617	denotes	annealed
T3286	6263-6268	PR:000005958	denotes	Csrp1
T3287	6348-6353	PR:000005958	denotes	Csrp1
T3288	6430-6435	PR:000005958	denotes	Csrp1
T3289	6512-6517	PR:000005958	denotes	Csrp1
T3290	6594-6598	PR:P04386	denotes	Gal4
T3291	6679-6684	NCBITaxon:10088	denotes	mouse
T3292	6685-6688	PR:000010925	denotes	Mt1
T3293	6689-6697	SO:0000167	denotes	promoter
T3295	6798-6807,6822-6830	SO:0000351	denotes	synthetic sequence
T3296	6812-6821	SO:0000993	denotes	consensus
T3297	6924-6929	PR:000011059	denotes	Ndrg1
T3298	7002-7007	PR:000011059	denotes	Ndrg1
T3299	7080-7085	PR:000011059	denotes	Ndrg1
T3300	7189-7194	PR:000014662	denotes	Sepw1
T3301	7284-7289	PR:000014662	denotes	Sepw1
T3302	7368-7376	PR:000015124	denotes	Slc39a10
T3303	7462-7470	PR:000015124	denotes	Slc39a10
T3304	7547-7568	PR:000000133	denotes	Specificity protein 1
T3305	7570-7573	PR:000000133	denotes	Sp1
T4144	7674-7683	UBERON:0000922	denotes	embryonic
T4145	7684-7695	CL:0000057	denotes	fibroblasts
T4146	7730-7734	PR:000010717	denotes	Mtf1
T4147	7734-7738	SO:0000346	denotes	loxP
T4148	7739-7744	NCBITaxon:10088	denotes	mouse
T4149	7745-7751	UBERON:0000922	denotes	embryo
T4150	7756-7761	GO:0040007	denotes	grown
T4151	7798-7804	NCBITaxon:27592	denotes	bovine
T4152	7805-7810	UBERON:0001977	denotes	serum
T4153	7827-7837	CHEBI:17334	denotes	penicillin
T4154	7838-7850	CHEBI:17076	denotes	streptomycin
T4155	7916-7929	CL:0000001	denotes	primary cells
T4156	7935-7946	GO:0009294	denotes	transfected
T4157	7964-7974	GO:0010467	denotes	expression
T4158	7975-7982	SO:0000155	denotes	plasmid
T4159	7994-8009	NCBITaxon:10633	denotes	simian virus 40
T4160	8011-8015	NCBITaxon:10633	denotes	SV40
T4161	8025-8032	CHEBI:59132	denotes	antigen
T4162	8047-8062	NCBITaxon:10358	denotes	cytomegalovirus
T4163	8064-8067	NCBITaxon:10358	denotes	CMV
T4164	8069-8077	SO:0000167	denotes	promoter
T4165	8100-8107	CHEBI:33893	denotes	reagent
T4166	8212-8217	NCBITaxon:10088	denotes	mouse
T4167	8218-8227	UBERON:0000922	denotes	embryonic
T4169	8218-8227,8239-8243	CL:0002321	denotes	embryonic cell
T4170	8228-8243	CL:0000057	denotes	fibroblast cell
T4171	8288-8292	PR:000010717	denotes	Mtf1
T4172	8292-8296	SO:0000346	denotes	loxP
T4173	8334-8341	SO:0001026	denotes	genomic
T4174	8363-8370	CHEBI:59132	denotes	antigen
T4175	8438-8449	GO:0009294	denotes	transfected
T4176	8457-8474	CHEBI:77635	denotes	calcium phosphate
T4177	8506-8516	GO:0010467	denotes	expression
T4178	8517-8524	SO:0000155	denotes	plasmid
T4179	8559-8562	NCBITaxon:10358	denotes	CMV
T4180	8563-8571	SO:0000167	denotes	promoter
T4181	8589-8599	GO:0010467	denotes	expression
T4182	8600-8607	SO:0000155	denotes	plasmid
T4183	8616-8624	CHEBI:7507	denotes	neomycin
T4184	8636-8640	SO:0000704	denotes	gene
T4185	8651-8658	GO:0065007	denotes	control
T4186	8669-8677	SO:0000167	denotes	promoter
T4187	8686-8697	GO:0009294	denotes	transfected
T4188	8747-8751	CHEBI:42768	denotes	G418
T4189	8820-8825	GO:0040007	denotes	grown
T4190	8849-8859	GO:0010467	denotes	expression
T4191	8895-8900	SO:0000147	denotes	exons
T4192	8912-8916	PR:000010717	denotes	Mtf1
T4193	9002-9006	PR:000010717	denotes	Mtf1
T2356	1490-1496	NCBITaxon:33208	denotes	Animal
T2357	1531-1535	PR:000010717	denotes	Mtf1
T2358	1535-1539	SO:0000346	denotes	loxP
T2359	1540-1544	SO:0000346	denotes	loxP
T2360	1545-1549	NCBITaxon:10088	denotes	mice
T2361	1564-1566	PR:000010788	denotes	Mx
T2362	1571-1580	SO:0000902	denotes	transgene
T2363	1582-1586	PR:000010717	denotes	Mtf1
T2364	1586-1590	SO:0000346	denotes	loxP
T2365	1591-1595	SO:0000346	denotes	loxP
T2366	1596-1598	PR:000010788	denotes	Mx
T2367	1611-1615	PR:000010717	denotes	Mtf1
T2368	1615-1617	PR:000010788	denotes	Mx
T2369	1655-1664	SO:0000902	denotes	transgene
T2370	1666-1670	PR:000010717	denotes	Mtf1
T3103	4539-4546	SO:0000112	denotes	primers
T3104	4548-4553	PR:000005958	denotes	Csrp1
T3105	4613-4624	CHEBI:17368	denotes	hypoxanthin
T3106	4724-4728	PR:000010717	denotes	Mtf1
T3107	4794-4799	PR:000011059	denotes	Ndrg1
T3108	4859-4864	PR:000014662	denotes	Sepw1
T3109	4924-4932	PR:000015124	denotes	Slc39a10
T3222	4993-5004	PR:000025471	denotes	S1 nuclease
T3223	5016-5027	SO:0000673	denotes	transcripts
T3224	5029-5031	PR:000025471	denotes	S1
T3225	5042-5044	PR:000025471	denotes	S1
T3226	5246-5248	PR:000025471	denotes	S1
T3227	5286-5289	CHEBI:37972	denotes	32P
T3228	5321-5323	PR:000025471	denotes	S1
T3229	5405-5410	PR:000014662	denotes	Sepw1
T3230	5411-5413	PR:000025471	denotes	S1
T4430	9117-9123	UBERON:0000479	denotes	tissue
T4431	9257-9287	CHEBI:28714	denotes	l-buthionine-[S,R]-sulfoximine
T4432	9289-9292	CHEBI:28714	denotes	BSO
T4433	9305-9309	CHEBI:23888	denotes	drug
T4434	9324-9335	CHEBI:16856	denotes	glutathione
T4435	9324-9345	GO:0006750	denotes	glutathione synthesis
T4436	9399-9404	CHEBI:35456	denotes	CdCl2
T4437	9503-9506	CHEBI:53233	denotes	MTT
T4438	9508-9568	CHEBI:53233	denotes	3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromid
T4439	9576-9594	GO:0008283	denotes	Cell Proliferation

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the bag model. You can change it to the chain model.

Id	Subject	Object	Predicate	Lexical cue
T2011	37-41	PR_EXT:000010717	denotes	Mtf1
T2012	63-67	NCBITaxon:10088	denotes	mice
T2013	72-77	UBERON:0002107	denotes	liver
T2014	87-95	SO_EXT:sequence_deletion_entity_or_process	denotes	deletion
T2015	96-100	PR_EXT:000010717	denotes	Mtf1
T2016	122-126	NCBITaxon:10088	denotes	mice
T2017	205-219	SO_EXT:0000040	denotes	genomic clones
T2018	231-236	SO_EXT:0000147	denotes	exons
T2019	247-251	PR_EXT:000010717	denotes	Mtf1
T2020	277-281	SO_EXT:0000704	denotes	gene
T2021	282-298	SO_EXT:0001644	denotes	targeting vector
T2022	314-327	GO_SO_EXT:sequence_rearrangement_process	denotes	recombination
T2024	372-380	SO_EXT:0005853	denotes	cassette
T2025	382-385	GO_EXT:phosphoglycerate_kinase	denotes	PGK
T2026	386-389	CHEBI_GO_EXT:neomycin_or_neomycin_phosphotransferase	denotes	neo
T2027	391-398	SO:0000357	denotes	flanked
T2028	406-416	SO_EXT:0000346	denotes	loxP sites
T2029	421-427	SO_EXT:sequence_cloning_process	denotes	cloned
T2030	453-457	SO_EXT:0000147	denotes	exon
T2031	463-467	PR_EXT:000010717	denotes	Mtf1
T2032	479-488	SO_EXT:0000346	denotes	loxP site
T2033	493-499	SO_EXT:sequence_cloning_process	denotes	cloned
T2034	525-529	SO_EXT:0000147	denotes	exon
T2035	535-544	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	thymidine
T2036	535-551	GO_PR_EXT:thymidine_kinase	denotes	thymidine kinase
T2037	553-555	GO_PR_EXT:thymidine_kinase	denotes	TK
T2038	557-565	SO_EXT:0005853	denotes	cassette
T2039	570-578	SO_EXT:sequence_insertion_process	denotes	inserted
T2040	602-606	SO_EXT:0000147	denotes	exon
T2041	614-622	CL:0002322	denotes	ES cells
T2042	617-622	CL_GO_EXT:cell	denotes	cells
T2043	652-662	SO_EXT:sequence_linearization	denotes	linearized
T2044	663-679	SO_EXT:0001644	denotes	targeting vector
T2045	709-713	CHEBI:42768	denotes	G418
T2046	813-823	GO:0010467	denotes	expression
T2047	831-842	GO_EXT:recombinase	denotes	recombinase
T2048	865-868	GO_EXT:phosphoglycerate_kinase	denotes	PGK
T2049	869-872	CHEBI_GO_EXT:neomycin_or_neomycin_phosphotransferase	denotes	neo
T2050	873-881	SO_EXT:0005853	denotes	cassette
T2051	887-891	NCBITaxon:10088	denotes	mice
T2052	905-913	SO_EXT:sequence_alteration_process	denotes	modified
T2053	914-918	PR_EXT:000010717	denotes	Mtf1
T2054	918-922	SO_EXT:0000346	denotes	loxP
T2055	923-929	SO_EXT:0001023	denotes	allele
T2056	970-976	SO_EXT:sequence_cloned_entity	denotes	clones
T2057	990-1001	UBERON:0000358	denotes	blastocysts
T2058	1058-1065	NCBITaxon:33208	denotes	animals
T2059	1067-1071	PR_EXT:000010717	denotes	Mtf1
T2060	1071-1075	SO_EXT:0000346	denotes	loxP
T2061	1076-1080	SO_EXT:0000346	denotes	loxP
T2062	1108-1119	GO_EXT:recombinase	denotes	recombinase
T2063	1120-1130	SO_EXT:transgenic_entity	denotes	transgenic
T2064	1136-1138	PR_EXT:000010788	denotes	Mx
T2065	1200-1205	UBERON:0002107	denotes	liver
T2066	1215-1219	PR_EXT:000010717	denotes	Mtf1
T2067	1239-1243	NCBITaxon:10088	denotes	mice
T2068	1286-1293	SO_EXT:0000112	denotes	primers
T2069	1312-1323	GO_EXT:recombinase	denotes	recombinase
T2070	1390-1394	PR_EXT:000010717	denotes	Mtf1
T2071	1394-1398	SO_EXT:0000346	denotes	loxP
T2072	1402-1411	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T2073	1412-1418	SO_EXT:0001023	denotes	allele
T2405	1666-1670	PR_EXT:000010717	denotes	Mtf1
T2406	1670-1674	SO_EXT:0000346	denotes	loxP
T2407	1675-1679	SO_EXT:0000346	denotes	loxP
T2408	1688-1692	PR_EXT:000010717	denotes	Mtf1
T2409	1692-1696	SO_EXT:0000346	denotes	loxP
T2410	1717-1727	UBERON:0001179	denotes	peritoneal
T2411	1764-1783	CHEBI_SO_EXT:double_stranded_RNA	denotes	double-stranded RNA
T2413	1784-1796,1811-1815	CHEBI:76777	denotes	polyinosinic acid
T2414	1788-1796	CHEBI_SO_EXT:inosine	denotes	inosinic
T2415	1797-1815	CHEBI_EXT:polyC_acid	denotes	polycytidylic acid
T2416	1817-1819	CHEBI:76777	denotes	pI
T2417	1820-1822	CHEBI_EXT:polyC_acid	denotes	pC
T2418	1852-1861	CHEBI_EXT:phosphate	denotes	phosphate
T2419	1862-1870	CHEBI_CHMO_EXT:buffer_process	denotes	buffered
T2420	1871-1877	CHEBI_EXT:saline_solution	denotes	saline
T2421	1925-1928	CHEBI_SO_EXT:DNA	denotes	DNA
T2422	1929-1936	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	binding
T2423	1954-1963	SO_EXT:biological_sequence	denotes	sequences
T2424	1969-1974	PR_EXT:000010717	denotes	MTF-1
T2425	1982-1986	SO_EXT:0000704	denotes	gene
T2426	2011-2015	NCBITaxon:10088	denotes	mice
T2427	2028-2030	CHEBI:76777	denotes	pI
T2428	2031-2033	CHEBI_EXT:polyC_acid	denotes	pC
T2429	2067-2072	CHEBI_EXT:metal	denotes	metal
T2430	2084-2088	NCBITaxon:10088	denotes	mice
T2431	2120-2122	CHEBI:76777	denotes	pI
T2432	2123-2125	CHEBI_EXT:polyC_acid	denotes	pC
T2433	2141-2150	UBERON_EXT:zone_of_skin_or_skin_of_body	denotes	cutaneous
T2434	2154-2156	UBERON_EXT:zone_of_skin_or_skin_of_body	denotes	c.
T2435	2189-2193	UBERON_EXT:body	denotes	body
T2436	2201-2206	CHEBI:50292	denotes	CdSO4
T2437	2213-2218	CHEBI:50292	denotes	CdSO4
T2438	2222-2225	CHEBI:15377	denotes	H2O
T2439	2227-2234	CHEBI_EXT:cadmium	denotes	cadmium
T2440	2258-2262	UBERON_EXT:body	denotes	body
T2441	2270-2273	CHEBI:15377	denotes	H2O
T2442	2302-2313	GO_EXT:killing	denotes	sacrificing
T2485	2367-2370	CHEBI_SO_EXT:RNA	denotes	RNA
T2486	2389-2394	UBERON:0002107	denotes	liver
T2487	2395-2401	UBERON:0000479	denotes	tissue
T2488	2405-2407	CHEBI:76777	denotes	pI
T2489	2408-2410	CHEBI_EXT:polyC_acid	denotes	pC
T2490	2429-2436	CHEBI_EXT:cadmium	denotes	cadmium
T2491	2445-2449	PR_EXT:000010717	denotes	Mtf1
T2492	2449-2451	PR_EXT:000010788	denotes	Mx
T2493	2460-2464	PR_EXT:000010717	denotes	Mtf1
T2494	2464-2468	SO_EXT:0000346	denotes	loxP
T2495	2469-2473	NCBITaxon:10088	denotes	mice
T2496	2485-2493	SO_EXT:genotype_or_entity_with_genotype	denotes	genotype
T2497	2524-2528	PATO_UBERON_EXT:male_or_bearer_of_maleness	denotes	male
T2498	2587-2591	SO_EXT:0000704	denotes	Gene
T2499	2587-2602	GO:0010467	denotes	Gene expression
T2500	2683-2688	NCBITaxon:10088	denotes	Mouse
T2501	2689-2695	SO_EXT:0001026	denotes	Genome
T2502	2787-2795	CHEBI_EXT:33893	denotes	reagents
T2503	2812-2816	SO_EXT:cDNA	denotes	cDNA
T2504	2851-2871	SO_EXT:double_stranded_complementary_DNA	denotes	Double-Stranded cDNA
T2505	2918-2921	CHEBI_SO_EXT:RNA	denotes	RNA
T2506	2932-2945	GO_EXT:transcription	denotes	transcription
T2507	2987-2990	CHEBI_SO_EXT:RNA	denotes	RNA
T2508	2991-3001	SO_EXT:0000673	denotes	Transcript
T2509	3002-3010	CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process	denotes	Labeling
T2510	3046-3050	SO_EXT:cDNA	denotes	cDNA
T2511	3069-3073	SO_EXT:cDNA	denotes	cDNA
T2512	3275-3280	CHEBI_SO_EXT:molecular_probe	denotes	probe
T2513	3476-3484	OBI_SO_EXT:gene_or_genetic_investigation_or_genetic_process	denotes	Genetics
T2514	3599-3606	CHEBI_EXT:cadmium	denotes	cadmium
T2515	3619-3624	PR_EXT:000010717	denotes	MTF-1
T2516	3637-3642	SO_EXT:0000704	denotes	genes
T2517	3644-3649	SO_EXT:0000704	denotes	Genes
T2518	3687-3696	GO:0010467	denotes	expressed
T2519	3742-3752	GO:0010467	denotes	expression
T2520	3797-3805	SO_EXT:genotype_or_entity_with_genotype	denotes	genotype
T2521	3904-3908	SO_EXT:0000704	denotes	gene
T2522	3948-3952	NCBITaxon:10088	denotes	mice
T2523	3990-3994	NCBITaxon:10088	denotes	mice
T2524	4061-4070	SO:0000993	denotes	consensus
T2525	4071-4079	SO_EXT:biological_sequence	denotes	sequence
T2526	4095-4103	SO_EXT:0000167	denotes	promoter
T2527	4116-4124	SO_EXT:sequence_upstreamness	denotes	upstream
T2528	4125-4134	SO_EXT:biological_sequence	denotes	sequences
T2529	4153-4158	SO_EXT:0000704	denotes	genes
T2530	4225-4231	SO_EXT:0001026	denotes	Genome
T3110	4282-4284	GO_EXT:reverse_transcription_or_reverse_transcriptase	denotes	RT
T3111	4293-4295	GO_EXT:reverse_transcription_or_reverse_transcriptase	denotes	RT
T3112	4337-4339	GO_EXT:reverse_transcription_or_reverse_transcriptase	denotes	RT
T3113	4420-4427	GO_PR_EXT:deoxyribonuclease_I	denotes	DNase I
T3114	4443-4446	CHEBI_SO_EXT:RNA	denotes	RNA
T3115	4448-4451	CHEBI_SO_EXT:RNA	denotes	RNA
T3116	4539-4546	SO_EXT:0000112	denotes	primers
T3117	4548-4553	PR_EXT:000005958	denotes	Csrp1
T3118	4613-4624	CHEBI:17368	denotes	hypoxanthin
T3119	4613-4657	GO_PR_EXT:hypoxanthine_guanine_phosphoribosyltransferase	denotes	hypoxanthin-guanin-phosphoribosyltransferase
T3120	4625-4631	CHEBI_EXT:guanine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	guanin
T3121	4659-4663	GO_PR_EXT:hypoxanthine_guanine_phosphoribosyltransferase	denotes	Hprt
T3122	4724-4728	PR_EXT:000010717	denotes	Mtf1
T3123	4794-4799	PR_EXT:000011059	denotes	Ndrg1
T3124	4859-4864	PR_EXT:000014662	denotes	Sepw1
T3125	4924-4932	PR_EXT:000015124	denotes	Slc39a10
T3231	4993-5004	PR_EXT:000025471	denotes	S1 nuclease
T3232	4996-5004	GO_EXT:0004518	denotes	nuclease
T3233	5016-5027	SO_EXT:0000673	denotes	transcripts
T3234	5029-5031	PR_EXT:000025471	denotes	S1
T3235	5042-5044	PR_EXT:000025471	denotes	S1
T3236	5111-5118	GO_PR_EXT:deoxyribonuclease_I	denotes	DNase I
T3237	5134-5137	CHEBI_SO_EXT:RNA	denotes	RNA
T3238	5139-5142	CHEBI_SO_EXT:RNA	denotes	RNA
T3239	5225-5234	CHEBI_EXT:polyatomic_entity_or_group	denotes	Molecular
T3240	5246-5248	PR_EXT:000025471	denotes	S1
T3241	5286-5289	CHEBI:37972	denotes	32P
T3242	5290-5297	CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process	denotes	labeled
T3243	5298-5314	CHEBI_SO_EXT:oligonucleotide	denotes	oligonucleotides
T3244	5316-5320	GO_PR_EXT:hypoxanthine_guanine_phosphoribosyltransferase	denotes	Hprt
T3245	5321-5323	PR_EXT:000025471	denotes	S1
T3246	5405-5410	PR_EXT:000014662	denotes	Sepw1
T3247	5411-5413	PR_EXT:000025471	denotes	S1
T3306	5558-5565	CHEBI_PR_EXT:protein	denotes	Protein
T3307	5585-5590	UBERON:0002107	denotes	liver
T3308	5591-5597	UBERON:0000479	denotes	tissue
T3309	5610-5616	UBERON:0000479	denotes	Tissue
T3310	5617-5624	CHEBI_PR_EXT:protein	denotes	Protein
T3311	5636-5643	CHEBI_EXT:33893	denotes	Reagent
T3312	5721-5726	NCBITaxon:10088	denotes	mouse
T3313	5727-5733	UBERON:0000479	denotes	tissue
T3314	5748-5755	CHEBI_EXT:33893	denotes	reagent
T3315	5827-5834	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	binding
T3316	5885-5888	CHEBI:37972	denotes	32P
T3317	5893-5900	CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process	denotes	labeled
T3318	5901-5933	SO_EXT:0000442	denotes	double-stranded oligonucleotides
T3319	5917-5933	CHEBI_SO_EXT:oligonucleotide	denotes	oligonucleotides
T3320	5953-5958	UBERON:0002107	denotes	liver
T3321	5959-5966	CHEBI_PR_EXT:protein	denotes	protein
T3322	6017-6024	CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process	denotes	labeled
T3323	6036-6051	CHEBI_SO_EXT:oligonucleotide	denotes	oligonucleotide
T3324	6069-6076	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	binding
T3325	6169-6178	CHEBI_EXT:polyatomic_entity_or_group	denotes	Molecular
T3326	6204-6220	CHEBI_SO_EXT:oligonucleotide	denotes	oligonucleotides
T3327	6226-6234	GO:0097617	denotes	annealed
T3328	6263-6268	PR_EXT:000005958	denotes	Csrp1
T3329	6348-6353	PR_EXT:000005958	denotes	Csrp1
T3330	6430-6435	PR_EXT:000005958	denotes	Csrp1
T3331	6512-6517	PR_EXT:000005958	denotes	Csrp1
T3332	6594-6598	PR_EXT:P04386	denotes	Gal4
T3333	6679-6684	NCBITaxon:10088	denotes	mouse
T3334	6685-6688	PR_EXT:000010925	denotes	Mt1
T3335	6689-6697	SO_EXT:0000167	denotes	promoter
T3337	6798-6807,6822-6830	SO:0000351	denotes	synthetic sequence
T3338	6812-6821	SO:0000993	denotes	consensus
T3339	6924-6929	PR_EXT:000011059	denotes	Ndrg1
T3340	7002-7007	PR_EXT:000011059	denotes	Ndrg1
T3341	7080-7085	PR_EXT:000011059	denotes	Ndrg1
T3342	7189-7194	PR_EXT:000014662	denotes	Sepw1
T3343	7284-7289	PR_EXT:000014662	denotes	Sepw1
T3344	7368-7376	PR_EXT:000015124	denotes	Slc39a10
T3345	7462-7470	PR_EXT:000015124	denotes	Slc39a10
T3346	7547-7568	PR_EXT:000000133	denotes	Specificity protein 1
T3347	7559-7566	CHEBI_PR_EXT:protein	denotes	protein
T3348	7570-7573	PR_EXT:000000133	denotes	Sp1
T3349	7575-7582	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	binding
T3350	7583-7591	SO_EXT:biological_sequence	denotes	sequence
T4194	7653-7657	CL_GO_EXT:cell	denotes	Cell
T4195	7674-7683	UBERON:0000922	denotes	embryonic
T4196	7684-7695	CL:0000057	denotes	fibroblasts
T4197	7730-7734	PR_EXT:000010717	denotes	Mtf1
T4198	7734-7738	SO_EXT:0000346	denotes	loxP
T4199	7739-7744	NCBITaxon:10088	denotes	mouse
T4200	7745-7751	UBERON:0000922	denotes	embryo
T4201	7756-7761	GO:0040007	denotes	grown
T4202	7798-7804	NCBITaxon:27592	denotes	bovine
T4203	7805-7810	UBERON:0001977	denotes	serum
T4204	7827-7837	CHEBI:17334	denotes	penicillin
T4205	7838-7850	CHEBI:17076	denotes	streptomycin
T4206	7872-7883	CHEBI_EXT:L_glutamine	denotes	l-glutamine
T4207	7874-7883	CHEBI_SO_EXT:glutamine	denotes	glutamine
T4208	7916-7929	CL:0000001	denotes	primary cells
T4209	7924-7929	CL_GO_EXT:cell	denotes	cells
T4210	7935-7946	GO:0009294	denotes	transfected
T4211	7964-7974	GO:0010467	denotes	expression
T4212	7975-7982	SO_EXT:0000155	denotes	plasmid
T4213	7983-7989	SO_EXT:sequence_coding_function	denotes	coding
T4214	7994-8009	NCBITaxon:10633	denotes	simian virus 40
T4215	8011-8015	NCBITaxon:10633	denotes	SV40
T4216	8025-8032	CHEBI_EXT:59132	denotes	antigen
T4217	8047-8062	NCBITaxon:10358	denotes	cytomegalovirus
T4218	8064-8067	NCBITaxon:10358	denotes	CMV
T4219	8069-8077	SO_EXT:0000167	denotes	promoter
T4220	8100-8107	CHEBI_EXT:33893	denotes	reagent
T4221	8167-8171	CL_GO_EXT:cell	denotes	Cell
T4222	8212-8217	NCBITaxon:10088	denotes	mouse
T4223	8218-8227	UBERON:0000922	denotes	embryonic
T4225	8218-8227,8239-8243	CL:0002321	denotes	embryonic cell
T4226	8228-8243	CL:0000057	denotes	fibroblast cell
T4227	8239-8243	CL_GO_EXT:cell	denotes	cell
T4228	8288-8292	PR_EXT:000010717	denotes	Mtf1
T4229	8292-8296	SO_EXT:0000346	denotes	loxP
T4230	8297-8305	SO_EXT:genotype_or_entity_with_genotype	denotes	genotype
T4231	8334-8341	SO_EXT:0001026	denotes	genomic
T4232	8363-8370	CHEBI_EXT:59132	denotes	antigen
T4233	8419-8424	CL_GO_EXT:cell	denotes	cells
T4234	8438-8449	GO:0009294	denotes	transfected
T4235	8457-8474	CHEBI:77635	denotes	calcium phosphate
T4236	8506-8516	GO:0010467	denotes	expression
T4237	8517-8524	SO_EXT:0000155	denotes	plasmid
T4238	8533-8544	GO_EXT:recombinase	denotes	recombinase
T4239	8559-8562	NCBITaxon:10358	denotes	CMV
T4240	8563-8571	SO_EXT:0000167	denotes	promoter
T4241	8589-8599	GO:0010467	denotes	expression
T4242	8600-8607	SO_EXT:0000155	denotes	plasmid
T4243	8616-8624	CHEBI:7507	denotes	neomycin
T4244	8636-8640	SO_EXT:0000704	denotes	gene
T4245	8651-8658	GO:0065007	denotes	control
T4246	8666-8668	GO_PR_EXT:thymidine_kinase	denotes	TK
T4247	8669-8677	SO_EXT:0000167	denotes	promoter
T4248	8686-8697	GO:0009294	denotes	transfected
T4249	8698-8703	CL_GO_EXT:cell	denotes	cells
T4250	8747-8751	CHEBI:42768	denotes	G418
T4251	8775-8781	SO_EXT:sequence_cloned_entity	denotes	clones
T4252	8795-8800	CL_GO_EXT:cell	denotes	cells
T4253	8820-8825	GO:0040007	denotes	grown
T4254	8849-8859	GO:0010467	denotes	expression
T4255	8867-8878	GO_EXT:recombinase	denotes	recombinase
T4256	8883-8891	SO_EXT:sequence_deletion_process	denotes	excision
T4257	8895-8900	SO_EXT:0000147	denotes	exons
T4258	8912-8916	PR_EXT:000010717	denotes	Mtf1
T4259	8934-8936	GO_EXT:reverse_transcription_or_reverse_transcriptase	denotes	RT
T4260	8946-8950	CL_GO_EXT:cell	denotes	cell
T4261	8990-8998	SO_EXT:sequence_deletion_entity_or_process	denotes	deletion
T4262	9002-9006	PR_EXT:000010717	denotes	Mtf1
T4440	9083-9088	CL_GO_EXT:cell	denotes	cells
T4441	9117-9123	UBERON:0000479	denotes	tissue
T4442	9154-9160	GO_PATO_EXT:biological_adhesion_or_attachedness	denotes	adhere
T4443	9175-9180	CL_GO_EXT:cell	denotes	cells
T4444	9257-9287	CHEBI:28714	denotes	l-buthionine-[S,R]-sulfoximine
T4445	9289-9292	CHEBI:28714	denotes	BSO
T4446	9305-9309	CHEBI_EXT:23888	denotes	drug
T4447	9324-9335	CHEBI:16856	denotes	glutathione
T4448	9324-9345	GO:0006750	denotes	glutathione synthesis
T4449	9359-9364	CL_GO_EXT:cell	denotes	cells
T4450	9399-9404	CHEBI:35456	denotes	CdCl2
T4451	9503-9506	CHEBI:53233	denotes	MTT
T4452	9508-9568	CHEBI:53233	denotes	3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromid
T4453	9576-9580	CL_GO_EXT:cell	denotes	Cell
T4454	9576-9594	GO:0008283	denotes	Cell Proliferation
T2390	1490-1496	NCBITaxon:33208	denotes	Animal
T2391	1526-1530	PATO_UBERON_EXT:male_or_bearer_of_maleness	denotes	male
T2392	1531-1535	PR_EXT:000010717	denotes	Mtf1
T2393	1535-1539	SO_EXT:0000346	denotes	loxP
T2394	1540-1544	SO_EXT:0000346	denotes	loxP
T2395	1545-1549	NCBITaxon:10088	denotes	mice
T2396	1564-1566	PR_EXT:000010788	denotes	Mx
T2397	1571-1580	SO_EXT:0000902	denotes	transgene
T2398	1582-1586	PR_EXT:000010717	denotes	Mtf1
T2399	1586-1590	SO_EXT:0000346	denotes	loxP
T2400	1591-1595	SO_EXT:0000346	denotes	loxP
T2401	1596-1598	PR_EXT:000010788	denotes	Mx
T2402	1611-1615	PR_EXT:000010717	denotes	Mtf1
T2403	1615-1617	PR_EXT:000010788	denotes	Mx
T2404	1655-1664	SO_EXT:0000902	denotes	transgene
T2023	352-360	CHEBI:7507	denotes	neomycin

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PMC:1253828 / 5385-15039 JSON TXT