PMC:1253828 / 21566-23118 JSONTXT

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This indicates that cadmium-induced expression of Ndrg1 depends on MTF-1.\nFive MRE core consensus sequences are located upstream of the mouse Ndrg1 transcription start (Figure 3b). Four of them are clustered (MRE1 to MRE4, −138 to −332 bp), the fifth one is located farther upstream (MRE5, −883 bp). EMSA was performed to test whether MTF-1 is interacting with some or all of the four proximal MRE sequences (Figure 3c). Separate oligonucleotides were tested for MRE1 and MRE2, whereas one oligonucleotide spanning both sequences was used for MRE3 and MRE4 (MRE3,4). No complex was seen with MRE1, but specific MTF-1 complexes were observed for both the MRE2 and MRE3,4 oligonucleotides with liver protein extract from an Mtf1loxP mouse. 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