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a second approach, the gene expression profile in livers of the above mentioned mice was compared by Affymetrix GeneChip® Mouse Genome 430 2.0 Arrays (Table 1). When analyzing the probe array data of livers from mock-treated Mtf1Mx-cre and Mtf1loxP mice, an at least 2-fold, reliable downregulation of expression was detected in Mtf1Mx-cre livers for 13 Affymetrix GeneChip® probe sets corresponding to 11 characterized genes (Table 1, a). Seven of these genes contain one or more MRE core consensus sequence TGCRCNC within a segment of 1000 bp upstream of the transcription start. For 26 probe sets corresponding to 24 different characterized genes, a 2-fold or higher, reliable upregulation was detected in Mtf1Mx-cre livers (Table 1, b); 17 of these 24 genes contain MRE core consensus sequences in the upstream region. The data set for livers of cadmium-treated Mtf1Mx-cre and Mtf1loxP mice revealed an at least 2-fold, reliable downregulation in Mtf1Mx-cre livers for 21 probe sets corresponding to 16 different characterized genes (Table 1, c); 10 of these contain MRE core consensus sequences in their upstream region. For 9 probe sets corresponding to 9 different characterized genes, an at least 2-fold, reliable upregulation was detected (Table 1, d); five of them contain MRE motifs. In addition to characterized genes, ESTs and RIKEN cDNA sequences were also found in the comparison of Mtf1Mx-cre and Mtf1loxP livers to be differentially expressed (Supplementary Table 1). Downregulation of Mt1 and Mt2 was detected in Mtf1Mx-cre livers for both conditions (though the level of significance for the downregulation of Mt1 in mock-treated animals was above 0.05; data not shown)."}

{"project":"craft-ca-core-dev","denotations":[{"id":"T5150","span":{"begin":26,"end":30},"obj":"SO:0000704"},{"id":"T5151","span":{"begin":26,"end":41},"obj":"GO:0010467"},{"id":"T5152","span":{"begin":53,"end":59},"obj":"UBERON:0002107"},{"id":"T5153","span":{"begin":83,"end":87},"obj":"NCBITaxon:10088"},{"id":"T5154","span":{"begin":125,"end":130},"obj":"NCBITaxon:10088"},{"id":"T5155","span":{"begin":131,"end":137},"obj":"SO:0001026"},{"id":"T5156","span":{"begin":203,"end":209},"obj":"UBERON:0002107"},{"id":"T5157","span":{"begin":228,"end":232},"obj":"PR:000010717"},{"id":"T5158","span":{"begin":232,"end":234},"obj":"PR:000010788"},{"id":"T5159","span":{"begin":243,"end":247},"obj":"PR:000010717"},{"id":"T5160","span":{"begin":247,"end":251},"obj":"SO:0000346"},{"id":"T5161","span":{"begin":252,"end":256},"obj":"NCBITaxon:10088"},{"id":"T5162","span":{"begin":287,"end":315},"obj":"GO:0010629"},{"id":"T5163","span":{"begin":332,"end":336},"obj":"PR:000010717"},{"id":"T5164","span":{"begin":336,"end":338},"obj":"PR:000010788"},{"id":"T5165","span":{"begin":343,"end":349},"obj":"UBERON:0002107"},{"id":"T5166","span":{"begin":423,"end":428},"obj":"SO:0000704"},{"id":"T5167","span":{"begin":458,"end":463},"obj":"SO:0000704"},{"id":"T5168","span":{"begin":493,"end":502},"obj":"SO:0000993"},{"id":"T5169","span":{"begin":545,"end":547},"obj":"SO:0000028"},{"id":"T5170","span":{"begin":647,"end":652},"obj":"SO:0000704"},{"id":"T5171","span":{"begin":712,"end":716},"obj":"PR:000010717"},{"id":"T5172","span":{"begin":716,"end":718},"obj":"PR:000010788"},{"id":"T5173","span":{"begin":723,"end":729},"obj":"UBERON:0002107"},{"id":"T5174","span":{"begin":759,"end":764},"obj":"SO:0000704"},{"id":"T5175","span":{"begin":782,"end":791},"obj":"SO:0000993"},{"id":"T5176","span":{"begin":843,"end":849},"obj":"UBERON:0002107"},{"id":"T5177","span":{"begin":869,"end":873},"obj":"PR:000010717"},{"id":"T5178","span":{"begin":873,"end":875},"obj":"PR:000010788"},{"id":"T5179","span":{"begin":884,"end":888},"obj":"PR:000010717"},{"id":"T5180","span":{"begin":888,"end":892},"obj":"SO:0000346"},{"id":"T5181","span":{"begin":893,"end":897},"obj":"NCBITaxon:10088"},{"id":"T5182","span":{"begin":954,"end":958},"obj":"PR:000010717"},{"id":"T5183","span":{"begin":958,"end":960},"obj":"PR:000010788"},{"id":"T5184","span":{"begin":965,"end":971},"obj":"UBERON:0002107"},{"id":"T5185","span":{"begin":1034,"end":1039},"obj":"SO:0000704"},{"id":"T5186","span":{"begin":1083,"end":1092},"obj":"SO:0000993"},{"id":"T5187","span":{"begin":1189,"end":1194},"obj":"SO:0000704"},{"id":"T5188","span":{"begin":1327,"end":1332},"obj":"SO:0000704"},{"id":"T5189","span":{"begin":1334,"end":1338},"obj":"SO:0000345"},{"id":"T5190","span":{"begin":1401,"end":1405},"obj":"PR:000010717"},{"id":"T5191","span":{"begin":1405,"end":1407},"obj":"PR:000010788"},{"id":"T5192","span":{"begin":1416,"end":1420},"obj":"PR:000010717"},{"id":"T5193","span":{"begin":1420,"end":1424},"obj":"SO:0000346"},{"id":"T5194","span":{"begin":1425,"end":1431},"obj":"UBERON:0002107"},{"id":"T5195","span":{"begin":1453,"end":1462},"obj":"GO:0010467"},{"id":"T5196","span":{"begin":1506,"end":1509},"obj":"PR:000010925"},{"id":"T5197","span":{"begin":1514,"end":1517},"obj":"PR:000010704"},{"id":"T5198","span":{"begin":1534,"end":1538},"obj":"PR:000010717"},{"id":"T5199","span":{"begin":1538,"end":1540},"obj":"PR:000010788"},{"id":"T5200","span":{"begin":1545,"end":1551},"obj":"UBERON:0002107"},{"id":"T5201","span":{"begin":1632,"end":1635},"obj":"PR:000010925"},{"id":"T5202","span":{"begin":1652,"end":1659},"obj":"NCBITaxon:33208"}],"text":"In a second approach, the gene expression profile in livers of the above mentioned mice was compared by Affymetrix GeneChip® Mouse Genome 430 2.0 Arrays (Table 1). When analyzing the probe array data of livers from mock-treated Mtf1Mx-cre and Mtf1loxP mice, an at least 2-fold, reliable downregulation of expression was detected in Mtf1Mx-cre livers for 13 Affymetrix GeneChip® probe sets corresponding to 11 characterized genes (Table 1, a). Seven of these genes contain one or more MRE core consensus sequence TGCRCNC within a segment of 1000 bp upstream of the transcription start. For 26 probe sets corresponding to 24 different characterized genes, a 2-fold or higher, reliable upregulation was detected in Mtf1Mx-cre livers (Table 1, b); 17 of these 24 genes contain MRE core consensus sequences in the upstream region. The data set for livers of cadmium-treated Mtf1Mx-cre and Mtf1loxP mice revealed an at least 2-fold, reliable downregulation in Mtf1Mx-cre livers for 21 probe sets corresponding to 16 different characterized genes (Table 1, c); 10 of these contain MRE core consensus sequences in their upstream region. For 9 probe sets corresponding to 9 different characterized genes, an at least 2-fold, reliable upregulation was detected (Table 1, d); five of them contain MRE motifs. In addition to characterized genes, ESTs and RIKEN cDNA sequences were also found in the comparison of Mtf1Mx-cre and Mtf1loxP livers to be differentially expressed (Supplementary Table 1). Downregulation of Mt1 and Mt2 was detected in Mtf1Mx-cre livers for both conditions (though the level of significance for the downregulation of Mt1 in mock-treated animals was above 0.05; data not shown)."}

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{"begin":1514,"end":1517},"obj":"PR_EXT:000010704"},{"id":"T5309","span":{"begin":1534,"end":1538},"obj":"PR_EXT:000010717"},{"id":"T5310","span":{"begin":1538,"end":1540},"obj":"PR_EXT:000010788"},{"id":"T5311","span":{"begin":1545,"end":1551},"obj":"UBERON:0002107"},{"id":"T5312","span":{"begin":1614,"end":1628},"obj":"GO_EXT:negative_regulation"},{"id":"T5313","span":{"begin":1632,"end":1635},"obj":"PR_EXT:000010925"},{"id":"T5314","span":{"begin":1652,"end":1659},"obj":"NCBITaxon:33208"}],"text":"In a second approach, the gene expression profile in livers of the above mentioned mice was compared by Affymetrix GeneChip® Mouse Genome 430 2.0 Arrays (Table 1). When analyzing the probe array data of livers from mock-treated Mtf1Mx-cre and Mtf1loxP mice, an at least 2-fold, reliable downregulation of expression was detected in Mtf1Mx-cre livers for 13 Affymetrix GeneChip® probe sets corresponding to 11 characterized genes (Table 1, a). Seven of these genes contain one or more MRE core consensus sequence TGCRCNC within a segment of 1000 bp upstream of the transcription start. For 26 probe sets corresponding to 24 different characterized genes, a 2-fold or higher, reliable upregulation was detected in Mtf1Mx-cre livers (Table 1, b); 17 of these 24 genes contain MRE core consensus sequences in the upstream region. The data set for livers of cadmium-treated Mtf1Mx-cre and Mtf1loxP mice revealed an at least 2-fold, reliable downregulation in Mtf1Mx-cre livers for 21 probe sets corresponding to 16 different characterized genes (Table 1, c); 10 of these contain MRE core consensus sequences in their upstream region. For 9 probe sets corresponding to 9 different characterized genes, an at least 2-fold, reliable upregulation was detected (Table 1, d); five of them contain MRE motifs. In addition to characterized genes, ESTs and RIKEN cDNA sequences were also found in the comparison of Mtf1Mx-cre and Mtf1loxP livers to be differentially expressed (Supplementary Table 1). Downregulation of Mt1 and Mt2 was detected in Mtf1Mx-cre livers for both conditions (though the level of significance for the downregulation of Mt1 in mock-treated animals was above 0.05; data not shown)."}