Id |
Subject |
Object |
Predicate |
Lexical cue |
T1550 |
0-4 |
RB |
denotes |
Here |
T1551 |
8-14 |
VBP |
denotes |
report |
T1552 |
5-7 |
PRP |
denotes |
we |
T1553 |
15-18 |
DT |
denotes |
the |
T1554 |
31-38 |
NNS |
denotes |
results |
T1555 |
19-30 |
JJ |
denotes |
preliminary |
T1556 |
39-41 |
IN |
denotes |
of |
T1557 |
42-45 |
DT |
denotes |
the |
T1558 |
63-69 |
NN |
denotes |
screen |
T1559 |
46-51 |
JJ |
denotes |
first |
T1560 |
52-62 |
JJ |
denotes |
systematic |
T1561 |
70-73 |
IN |
denotes |
for |
T1562 |
74-82 |
NN |
denotes |
proteins |
T1563 |
83-87 |
WDT |
denotes |
that |
T1564 |
88-96 |
VBP |
denotes |
interact |
T1565 |
97-101 |
IN |
denotes |
with |
T1566 |
102-106 |
NN |
denotes |
PAX6 |
T1567 |
106-107 |
. |
denotes |
. |
T1568 |
107-273 |
sentence |
denotes |
We used sequence alignment algorithms and secondary structure prediction programs to define a new domain of 32 amino acids at the C-terminal end of the PAX6 protein. |
T1569 |
108-110 |
PRP |
denotes |
We |
T1570 |
111-115 |
VBD |
denotes |
used |
T1571 |
116-124 |
NN |
denotes |
sequence |
T1572 |
125-134 |
NN |
denotes |
alignment |
T1573 |
135-145 |
NNS |
denotes |
algorithms |
T1574 |
146-149 |
CC |
denotes |
and |
T1575 |
150-159 |
JJ |
denotes |
secondary |
T1576 |
160-169 |
NN |
denotes |
structure |
T1577 |
170-180 |
NN |
denotes |
prediction |
T1578 |
181-189 |
NNS |
denotes |
programs |
T1579 |
190-192 |
TO |
denotes |
to |
T1580 |
193-199 |
VB |
denotes |
define |
T1581 |
200-201 |
DT |
denotes |
a |
T1582 |
206-212 |
NN |
denotes |
domain |
T1583 |
202-205 |
JJ |
denotes |
new |
T1584 |
213-215 |
IN |
denotes |
of |
T1585 |
216-218 |
CD |
denotes |
32 |
T1586 |
225-230 |
NNS |
denotes |
acids |
T1587 |
219-224 |
NN |
denotes |
amino |
T1588 |
231-233 |
IN |
denotes |
at |
T1589 |
234-237 |
DT |
denotes |
the |
T1590 |
249-252 |
NN |
denotes |
end |
T1591 |
238-239 |
NN |
denotes |
C |
T1592 |
240-248 |
JJ |
denotes |
terminal |
T1593 |
239-240 |
HYPH |
denotes |
- |
T1594 |
253-255 |
IN |
denotes |
of |
T1595 |
256-259 |
DT |
denotes |
the |
T1596 |
265-272 |
NN |
denotes |
protein |
T1597 |
260-264 |
NN |
denotes |
PAX6 |
T1598 |
272-273 |
. |
denotes |
. |
T1599 |
273-436 |
sentence |
denotes |
We then screened a brain library with this peptide using the yeast two-hybrid technique and identified three novel interacting proteins, HOMER3, DNCL1 and TRIM11. |
T1600 |
274-276 |
PRP |
denotes |
We |
T1601 |
282-290 |
VBD |
denotes |
screened |
T1602 |
277-281 |
RB |
denotes |
then |
T1603 |
291-292 |
DT |
denotes |
a |
T1604 |
299-306 |
NN |
denotes |
library |
T1605 |
293-298 |
NN |
denotes |
brain |
T1606 |
307-311 |
IN |
denotes |
with |
T1607 |
312-316 |
DT |
denotes |
this |
T1608 |
317-324 |
NN |
denotes |
peptide |
T1609 |
325-330 |
VBG |
denotes |
using |
T1610 |
331-334 |
DT |
denotes |
the |
T1611 |
352-361 |
NN |
denotes |
technique |
T1612 |
335-340 |
NN |
denotes |
yeast |
T1613 |
341-344 |
CD |
denotes |
two |
T1614 |
345-351 |
NN |
denotes |
hybrid |
T1615 |
344-345 |
HYPH |
denotes |
- |
T1616 |
362-365 |
CC |
denotes |
and |
T1617 |
366-376 |
VBD |
denotes |
identified |
T1618 |
377-382 |
CD |
denotes |
three |
T1619 |
401-409 |
NN |
denotes |
proteins |
T1620 |
383-388 |
JJ |
denotes |
novel |
T1621 |
389-400 |
VBG |
denotes |
interacting |
T1622 |
409-411 |
, |
denotes |
, |
T1623 |
411-417 |
NN |
denotes |
HOMER3 |
T1624 |
417-419 |
, |
denotes |
, |
T1625 |
419-424 |
NN |
denotes |
DNCL1 |
T1626 |
425-428 |
CC |
denotes |
and |
T1627 |
429-435 |
NN |
denotes |
TRIM11 |
T1628 |
435-436 |
. |
denotes |
. |
T1629 |
436-548 |
sentence |
denotes |
The interaction between PAX6 and these proteins was disrupted by naturally occurring C-terminal PAX6 mutations. |
T1630 |
437-440 |
DT |
denotes |
The |
T1631 |
441-452 |
NN |
denotes |
interaction |
T1632 |
489-498 |
VBN |
denotes |
disrupted |
T1633 |
453-460 |
IN |
denotes |
between |
T1634 |
461-465 |
NN |
denotes |
PAX6 |
T1635 |
466-469 |
CC |
denotes |
and |
T1636 |
470-475 |
DT |
denotes |
these |
T1637 |
476-484 |
NN |
denotes |
proteins |
T1638 |
485-488 |
VBD |
denotes |
was |
T1639 |
499-501 |
IN |
denotes |
by |
T1640 |
502-511 |
RB |
denotes |
naturally |
T1641 |
512-521 |
VBG |
denotes |
occurring |
T1642 |
538-547 |
NNS |
denotes |
mutations |
T1643 |
522-523 |
NN |
denotes |
C |
T1644 |
524-532 |
JJ |
denotes |
terminal |
T1645 |
523-524 |
HYPH |
denotes |
- |
T1646 |
533-537 |
NN |
denotes |
PAX6 |
T1647 |
547-548 |
. |
denotes |
. |
R913 |
T1613 |
T1614 |
nummod |
two,hybrid |
R916 |
T1614 |
T1611 |
compound |
hybrid,technique |
R917 |
T1615 |
T1614 |
punct |
-,hybrid |
R919 |
T1616 |
T1601 |
cc |
and,screened |
R920 |
T1617 |
T1601 |
conj |
identified,screened |
R921 |
T1618 |
T1619 |
nummod |
three,proteins |
R923 |
T1619 |
T1617 |
dobj |
proteins,identified |
R924 |
T1620 |
T1619 |
amod |
novel,proteins |
R926 |
T1621 |
T1619 |
amod |
interacting,proteins |
R927 |
T1622 |
T1619 |
punct |
", ",proteins |
R928 |
T1623 |
T1619 |
appos |
HOMER3,proteins |
R929 |
T1624 |
T1623 |
punct |
", ",HOMER3 |
R930 |
T1625 |
T1623 |
conj |
DNCL1,HOMER3 |
R931 |
T1626 |
T1625 |
cc |
and,DNCL1 |
R933 |
T1627 |
T1625 |
conj |
TRIM11,DNCL1 |
R934 |
T1628 |
T1601 |
punct |
.,screened |
R936 |
T1630 |
T1631 |
det |
The,interaction |
R937 |
T1631 |
T1632 |
nsubjpass |
interaction,disrupted |
R939 |
T1633 |
T1631 |
prep |
between,interaction |
R940 |
T1634 |
T1633 |
pobj |
PAX6,between |
R942 |
T1635 |
T1634 |
cc |
and,PAX6 |
R943 |
T1636 |
T1637 |
det |
these,proteins |
R945 |
T1637 |
T1634 |
conj |
proteins,PAX6 |
R946 |
T1638 |
T1632 |
auxpass |
was,disrupted |
R947 |
T1639 |
T1632 |
agent |
by,disrupted |
R949 |
T1640 |
T1641 |
advmod |
naturally,occurring |
R950 |
T1641 |
T1642 |
amod |
occurring,mutations |
R953 |
T1642 |
T1639 |
pobj |
mutations,by |
R954 |
T1643 |
T1644 |
npadvmod |
C,terminal |
R956 |
T1644 |
T1642 |
amod |
terminal,mutations |
R958 |
T1645 |
T1644 |
punct |
-,terminal |
R959 |
T1646 |
T1642 |
compound |
PAX6,mutations |
R960 |
T1647 |
T1632 |
punct |
.,disrupted |
R962 |
T1550 |
T1551 |
advmod |
Here,report |
R963 |
T1552 |
T1551 |
nsubj |
we,report |
R964 |
T1553 |
T1554 |
det |
the,results |
R965 |
T1554 |
T1551 |
dobj |
results,report |
R966 |
T1555 |
T1554 |
amod |
preliminary,results |
R967 |
T1556 |
T1554 |
prep |
of,results |
R968 |
T1557 |
T1558 |
det |
the,screen |
R969 |
T1558 |
T1556 |
pobj |
screen,of |
R970 |
T1559 |
T1558 |
amod |
first,screen |
R971 |
T1560 |
T1558 |
amod |
systematic,screen |
R972 |
T1561 |
T1558 |
prep |
for,screen |
R973 |
T1562 |
T1561 |
pobj |
proteins,for |
R974 |
T1563 |
T1564 |
dep |
that,interact |
R975 |
T1564 |
T1562 |
relcl |
interact,proteins |
R976 |
T1565 |
T1564 |
prep |
with,interact |
R977 |
T1566 |
T1565 |
pobj |
PAX6,with |
R978 |
T1567 |
T1551 |
punct |
.,report |
R979 |
T1569 |
T1570 |
nsubj |
We,used |
R980 |
T1571 |
T1572 |
compound |
sequence,alignment |
R981 |
T1572 |
T1573 |
compound |
alignment,algorithms |
R982 |
T1573 |
T1570 |
dobj |
algorithms,used |
R983 |
T1574 |
T1573 |
cc |
and,algorithms |
R984 |
T1575 |
T1576 |
amod |
secondary,structure |
R985 |
T1576 |
T1577 |
compound |
structure,prediction |
R986 |
T1577 |
T1578 |
compound |
prediction,programs |
R987 |
T1578 |
T1573 |
conj |
programs,algorithms |
R988 |
T1579 |
T1580 |
aux |
to,define |
R989 |
T1580 |
T1570 |
advcl |
define,used |
R990 |
T1581 |
T1582 |
det |
a,domain |
R991 |
T1582 |
T1580 |
dobj |
domain,define |
R992 |
T1583 |
T1582 |
amod |
new,domain |
R993 |
T1584 |
T1582 |
prep |
of,domain |
R994 |
T1585 |
T1586 |
nummod |
32,acids |
R995 |
T1586 |
T1584 |
pobj |
acids,of |
R996 |
T1587 |
T1586 |
compound |
amino,acids |
R997 |
T1588 |
T1580 |
prep |
at,define |
R998 |
T1589 |
T1590 |
det |
the,end |
R999 |
T1590 |
T1588 |
pobj |
end,at |
R1000 |
T1591 |
T1592 |
npadvmod |
C,terminal |
R1001 |
T1592 |
T1590 |
amod |
terminal,end |
R1002 |
T1593 |
T1592 |
punct |
-,terminal |
R1003 |
T1594 |
T1590 |
prep |
of,end |
R1004 |
T1595 |
T1596 |
det |
the,protein |
R1005 |
T1596 |
T1594 |
pobj |
protein,of |
R1006 |
T1597 |
T1596 |
compound |
PAX6,protein |
R1007 |
T1598 |
T1570 |
punct |
.,used |
R1008 |
T1600 |
T1601 |
nsubj |
We,screened |
R1009 |
T1602 |
T1601 |
advmod |
then,screened |
R1010 |
T1603 |
T1604 |
det |
a,library |
R1011 |
T1604 |
T1601 |
dobj |
library,screened |
R1012 |
T1605 |
T1604 |
compound |
brain,library |
R1013 |
T1606 |
T1601 |
prep |
with,screened |
R1014 |
T1607 |
T1608 |
det |
this,peptide |
R1015 |
T1608 |
T1606 |
pobj |
peptide,with |
R1016 |
T1609 |
T1601 |
advcl |
using,screened |
R1017 |
T1610 |
T1611 |
det |
the,technique |
R1018 |
T1611 |
T1609 |
dobj |
technique,using |
R1019 |
T1612 |
T1611 |
nmod |
yeast,technique |