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PMC:1208879 / 29878-31045 JSONTXT

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Id Subject Object Predicate Lexical cue
T8267 1166-1167 . denotes .
T8266 1165-1166 -RRB- denotes )
T8265 1158-1165 NNP denotes Promega
T8264 1157-1158 -LRB- denotes (
T8263 1144-1148 NN denotes size
T8262 1149-1156 NNS denotes markers
T8261 1136-1137 HYPH denotes /
T8260 1137-1143 NN denotes HaeIII
T8259 1131-1136 NN denotes ΦX174
T8258 1126-1130 IN denotes with
T8257 1114-1121 NN denotes agarose
T8256 1112-1113 NN denotes %
T8255 1109-1112 CD denotes 2.5
T8254 1122-1125 NN denotes gel
T8253 1107-1108 DT denotes a
T8252 1104-1106 IN denotes on
T8251 1090-1094 VBD denotes were
T8250 1095-1103 VBN denotes resolved
T8249 1081-1089 NNS denotes Products
T8248 1080-1167 sentence denotes Products were resolved on a 2.5% agarose gel with ΦX174/HaeIII size markers (Promega).
T8247 1079-1080 . denotes .
T8246 1078-1079 CD denotes 1
T8245 1076-1077 SYM denotes ×
T8244 1074-1075 -RRB- denotes )
T8243 1071-1074 NN denotes min
T8242 1069-1070 CD denotes 2
T8241 1065-1068 IN denotes for
T8240 1060-1062 CD denotes 72
T8239 1062-1064 NN denotes °C
T8238 1059-1060 -LRB- denotes (
T8237 1055-1058 CC denotes and
T8236 1052-1054 CD denotes 32
T8235 1050-1051 SYM denotes ×
T8234 1048-1049 -RRB- denotes )
T8233 1045-1048 NN denotes sec
T8232 1042-1044 CD denotes 30
T8231 1038-1041 IN denotes for
T8230 1035-1037 NN denotes °C
T8229 1033-1035 CD denotes 72
T8228 1031-1033 , denotes ,
T8227 1028-1031 NN denotes sec
T8226 1025-1027 CD denotes 30
T8225 1021-1024 IN denotes for
T8224 1018-1020 NN denotes °C
T8223 1016-1018 CD denotes 55
T8222 1014-1016 , denotes ,
T8221 1011-1014 NN denotes sec
T8220 1008-1010 CD denotes 30
T8219 1004-1007 IN denotes for
T8218 999-1001 CD denotes 94
T8217 1001-1003 NN denotes °C
T8216 998-999 -LRB- denotes (
T8215 996-998 , denotes ,
T8214 995-996 CD denotes 1
T8213 993-994 SYM denotes ×
T8212 991-992 -RRB- denotes )
T8211 988-991 NN denotes sec
T8210 985-987 CD denotes 30
T8209 981-984 IN denotes for
T8208 978-980 NN denotes °C
T8207 976-978 CD denotes 95
T8206 975-976 -LRB- denotes (
T8205 970-974 VBD denotes were
T8204 959-969 NNS denotes conditions
T8203 955-958 NN denotes PCR
T8202 954-1080 sentence denotes PCR conditions were (95°C for 30 sec) × 1, (94°C for 30 sec, 55°C for 30 sec, 72°C for 30 sec) × 32 and (72°C for 2 min) × 1.
T8201 953-954 . denotes .
T8200 952-953 -RRB- denotes )
T8199 940-946 NNP denotes Perkin
T8198 947-952 NNP denotes Elmer
T8197 939-940 -LRB- denotes (
T8196 928-938 NN denotes polymerase
T8195 919-927 NNP denotes AmpliTaq
T8194 916-918 IN denotes of
T8193 910-915 NNS denotes units
T8192 906-909 CD denotes 2.5
T8191 902-905 CC denotes and
T8190 890-894 DT denotes each
T8189 895-901 NN denotes primer
T8188 884-889 NN denotes 200μM
T8187 882-884 , denotes ,
T8186 877-882 NN denotes MgCl2
T8185 874-876 NN denotes mM
T8184 870-873 CD denotes 1.5
T8183 868-870 , denotes ,
T8182 867-868 -RRB- denotes )
T8181 855-861 NNP denotes Perkin
T8180 862-867 NNP denotes Elmer
T8179 854-855 -LRB- denotes (
T8178 836-846 NN denotes polymerase
T8177 827-835 NNP denotes AmpliTaq
T8176 826-827 SYM denotes x
T8175 847-853 NN denotes buffer
T8174 825-826 CD denotes 1
T8173 814-824 VBG denotes containing
T8172 805-813 NN denotes reaction
T8171 803-804 DT denotes a
T8170 800-802 IN denotes in
T8169 785-789 VBD denotes were
T8168 790-799 VBN denotes amplified
T8167 772-779 NN denotes library
T8166 780-784 NN denotes cDNA
T8165 769-771 NN denotes ng
T8164 766-768 CD denotes 10
T8163 765-954 sentence denotes 10 ng library cDNA were amplified in a reaction containing 1xAmpliTaq polymerase buffer (Perkin Elmer), 1.5 mM MgCl2, 200μM each primer and 2.5 units of AmpliTaq polymerase (Perkin Elmer).
T8162 764-765 . denotes .
T8161 759-762 NN denotes ATA
T8160 755-758 NN denotes GGG
T8159 751-754 NN denotes TCG
T8158 747-750 NN denotes GAT
T8157 743-746 NN denotes AGA
T8156 763-764 NN denotes A
T8155 739-742 NN denotes CAC
T8154 737-739 : denotes :
T8153 735-736 HYPH denotes -
T8152 736-737 NN denotes R
T8151 729-735 NN denotes Atp5a1
T8150 727-728 : denotes ;
T8149 721-724 NN denotes CTC
T8148 717-720 NN denotes GTC
T8147 713-716 NN denotes GAT
T8146 709-712 NN denotes TGA
T8145 705-708 NN denotes ACG
T8144 725-727 NN denotes CA
T8143 701-704 NN denotes CAC
T8142 699-701 : denotes :
T8141 697-698 HYPH denotes -
T8140 698-699 NN denotes F
T8139 691-697 NN denotes Atp5a1
T8138 689-690 : denotes ;
T8137 683-686 NN denotes GCC
T8136 679-682 NN denotes ACA
T8135 675-678 NN denotes CCC
T8134 671-674 NN denotes TTG
T8133 667-670 NN denotes ACC
T8132 687-689 NN denotes TT
T8131 663-666 NN denotes ATG
T8130 661-663 : denotes :
T8120 650-652 NN denotes GC
T8119 626-629 NN denotes CAT
T8118 624-626 : denotes :
T8117 622-623 HYPH denotes -
T8116 623-624 NN denotes F
T8115 617-622 NN denotes Gapdh
T8114 615-616 : denotes ;
T8113 608-611 NN denotes ATG
T8112 604-607 NN denotes AAA
T8111 600-603 NN denotes AAA
T8110 596-599 NN denotes GAG
T8109 592-595 NN denotes AGG
T8108 588-591 NN denotes CTA
T8107 612-615 NN denotes GGG
T8106 586-587 NN denotes R
T8105 585-586 HYPH denotes -
T8104 580-585 NN denotes Dncl1
T8103 578-579 : denotes ;
T8102 573-576 NNP denotes GTC
T8101 569-572 NNP denotes CAT
T8100 565-568 NNP denotes AGA
T8099 561-564 NN denotes TGC
T8098 557-560 NN denotes AAA
T8097 553-556 NN denotes CAA
T8096 577-578 NN denotes G
T8095 551-552 NN denotes F
T8094 550-551 HYPH denotes -
T8093 545-550 NN denotes Dncl1
T8092 543-544 : denotes ;
T8091 537-540 NN denotes TAG
T8090 533-536 NN denotes TCA
T8089 529-532 NN denotes TAG
T8088 525-528 NN denotes AGA
T8087 521-524 NN denotes TGC
T8086 517-520 NN denotes GCC
T8085 541-543 NN denotes GG
T8084 515-516 NN denotes R
T8083 514-515 HYPH denotes -
T8082 508-514 NN denotes Trim11
T8081 506-507 : denotes ;
T8080 499-502 NN denotes AAG
T8079 495-498 NN denotes GTG
T8078 491-494 NN denotes GAT
T8077 487-490 NN denotes CAG
T8076 483-486 NN denotes GTG
T8075 503-506 NN denotes CTG
T8074 481-482 NN denotes F
T8073 480-481 HYPH denotes -
T8072 474-480 NN denotes Trim11
T8071 472-473 : denotes ;
T8070 467-470 NN denotes TCA
T8069 463-466 NN denotes AGC
T8068 459-462 NN denotes CAA
T8067 455-458 NN denotes GTG
T8066 451-454 NN denotes ACA
T8065 447-450 NN denotes TAC
T8064 443-446 NN denotes CTC
T8063 471-472 NN denotes G
T8062 441-442 NN denotes R
T8061 440-441 HYPH denotes -
T8060 434-440 NN denotes Homer3
T8059 432-433 : denotes ;
T8058 425-428 NN denotes TAG
T8057 421-424 NN denotes CTG
T8056 417-420 NN denotes TGG
T8055 413-416 NN denotes AGG
T8054 409-412 NN denotes CCC
T8053 429-432 NN denotes AGC
T8052 407-408 NN denotes F
T8051 406-407 HYPH denotes -
T8050 400-406 NN denotes Homer3
T8049 398-399 : denotes ;
T8048 392-395 NN denotes TCC
T8047 388-391 NN denotes CTC
T8046 384-387 NN denotes GCT
T8045 380-383 NN denotes CAT
T8044 376-379 NN denotes TCA
T8043 372-375 NN denotes CGA
T8042 396-398 NN denotes TT
T8041 370-371 NN denotes R
T8040 369-370 HYPH denotes -
T8039 365-369 NN denotes Pax6
T8038 363-364 : denotes ;
T8037 356-359 NN denotes TAC
T8036 352-355 NN denotes ATC
T8035 348-351 NN denotes TAG
T8034 344-347 NN denotes AAA
T8033 340-343 NN denotes CCA
T8032 336-339 NN denotes CAG
T8031 360-363 NN denotes CTG
T8030 334-335 NN denotes F
T8029 333-334 HYPH denotes -
T8028 329-333 NN denotes Pax6
T8027 327-329 : denotes :
T8026 323-327 VBD denotes were
T8025 313-322 NNS denotes sequences
T8024 306-312 NN denotes Primer
T8023 305-765 sentence denotes Primer sequences were: Pax6-F CAG CCA AAA TAG ATC TAC CTG; Pax6-R CGA TCA CAT GCT CTC TCC TT; Homer3-F CCC AGG TGG CTG TAG AGC; Homer3-R CTC TAC ACA GTG CAA AGC TCA G; Trim11-F GTG CAG GAT GTG AAG CTG; Trim11-R GCC TGC AGA TAG TCA TAG GG; Dncl1-F CAA AAA TGC AGA CAT GTC G; Dncl1-R CTA AGG GAG AAA AAA ATG GGG; Gapdh-F: CAT CAC CAT CTT CCA GGA GC; Gapdh-R: ATG ACC TTG CCC ACA GCC TT; Atp5a1-F: CAC ACG TGA GAT GTC CTC CA; Atp5a1-R: CAC AGA GAT TCG GGG ATA A.
T8022 304-305 . denotes .
T8021 290-294 VBD denotes were
T8020 275-282 VBN denotes spliced
T8019 265-274 RB denotes correctly
T8018 283-289 NNS denotes clones
T8017 260-264 RB denotes only
T8016 255-259 IN denotes that
T8015 295-304 VBN denotes amplified
T8014 252-254 IN denotes so
T8013 250-252 , denotes ,
T8012 244-250 NN denotes intron
T8011 234-239 RBS denotes least
T8010 240-243 CD denotes one
T8009 231-233 RB denotes at
T8008 225-230 VB denotes cross
T8007 222-224 TO denotes to
T8006 208-212 VBD denotes were
T8005 213-221 VBN denotes designed
T8004 200-207 NNS denotes Primers
T8003 199-305 sentence denotes Primers were designed to cross at least one intron, so that only correctly spliced clones were amplified.
T8002 198-199 . denotes .
T8001 197-198 -RRB- denotes ]
T8000 194-195 , denotes ,
T7999 192-194 CD denotes 29
T7998 195-197 CD denotes 30
T7997 191-192 -LRB- denotes [
T7996 184-190 NN denotes Atp5a1
T7995 180-183 CC denotes and
T7994 174-179 NN denotes Gapdh
T7993 158-167 VBN denotes expressed
T7992 143-157 RB denotes constitutively
T7991 168-173 NNS denotes genes
T7990 139-142 DT denotes the
T7989 135-138 CC denotes and
T7988 128-134 NN denotes Trim11
T7987 126-128 , denotes ,
T7986 121-126 NN denotes Dncl1
T7985 119-121 , denotes ,
T7984 113-119 NN denotes Homer3
T7983 111-113 , denotes ,
T7982 107-111 NN denotes Pax6
T7981 104-106 IN denotes on
T7980 90-93 VBD denotes was
T7979 86-89 NN denotes PCR
T7978 68-85 JJ denotes semi-quantitative
T7977 66-68 , denotes ,
T7976 54-58 NN denotes cDNA
T7975 59-66 NN denotes library
T7974 50-53 DT denotes the
T7973 47-49 IN denotes in
T7972 40-46 NNS denotes clones
T7971 37-39 IN denotes of
T7970 13-21 JJ denotes relative
T7969 22-36 NN denotes representation
T7968 9-12 DT denotes the
T7967 94-103 VBN denotes performed
T7966 3-8 VB denotes check
T7965 0-2 TO denotes To
T8129 659-660 HYPH denotes -
T8128 660-661 NN denotes R
T8127 654-659 NN denotes Gapdh
T8126 652-653 : denotes ;
T8125 646-649 NN denotes GGA
T8124 642-645 NN denotes CCA
T8123 638-641 NN denotes CTT
T8122 634-637 NN denotes CAT
T8121 630-633 NN denotes CAC
R5009 T7965 T7966 aux To,check
R5010 T7966 T7967 advcl check,performed
R5011 T7968 T7969 det the,representation
R5012 T7969 T7966 dobj representation,check
R5013 T7970 T7969 amod relative,representation
R5014 T7971 T7969 prep of,representation
R5015 T7972 T7971 pobj clones,of
R5016 T7973 T7969 prep in,representation
R5017 T7974 T7975 det the,library
R5018 T7975 T7973 pobj library,in
R5019 T7976 T7975 compound cDNA,library
R5020 T7977 T7967 punct ", ",performed
R5021 T7978 T7979 amod semi-quantitative,PCR
R5022 T7979 T7967 nsubjpass PCR,performed
R5023 T7980 T7967 auxpass was,performed
R5024 T7981 T7967 prep on,performed
R5025 T7982 T7981 pobj Pax6,on
R5026 T7983 T7982 punct ", ",Pax6
R5027 T7984 T7982 conj Homer3,Pax6
R5028 T7985 T7984 punct ", ",Homer3
R5029 T7986 T7984 conj Dncl1,Homer3
R5030 T7987 T7986 punct ", ",Dncl1
R5031 T7988 T7986 conj Trim11,Dncl1
R5032 T7989 T7988 cc and,Trim11
R5033 T7990 T7991 det the,genes
R5034 T7991 T7988 conj genes,Trim11
R5035 T7992 T7993 advmod constitutively,expressed
R5036 T7993 T7991 amod expressed,genes
R5037 T7994 T7991 appos Gapdh,genes
R5038 T7995 T7994 cc and,Gapdh
R5039 T7996 T7994 conj Atp5a1,Gapdh
R5040 T7997 T7998 punct [,30
R5041 T7998 T7967 parataxis 30,performed
R5042 T7999 T7998 nummod 29,30
R5043 T8000 T7998 punct ",",30
R5044 T8001 T7998 punct ],30
R5045 T8002 T7967 punct .,performed
R5046 T8004 T8005 nsubjpass Primers,designed
R5047 T8006 T8005 auxpass were,designed
R5048 T8007 T8008 aux to,cross
R5049 T8008 T8005 advcl cross,designed
R5050 T8009 T8010 advmod at,one
R5051 T8010 T8012 nummod one,intron
R5052 T8011 T8010 advmod least,one
R5053 T8012 T8008 dobj intron,cross
R5054 T8013 T8005 punct ", ",designed
R5055 T8014 T8015 mark so,amplified
R5056 T8015 T8005 advcl amplified,designed
R5057 T8016 T8015 mark that,amplified
R5058 T8017 T8018 advmod only,clones
R5059 T8018 T8015 nsubjpass clones,amplified
R5060 T8019 T8020 advmod correctly,spliced
R5061 T8020 T8018 amod spliced,clones
R5062 T8021 T8015 auxpass were,amplified
R5063 T8022 T8005 punct .,designed
R5064 T8024 T8025 compound Primer,sequences
R5065 T8025 T8026 nsubj sequences,were
R5066 T8027 T8026 punct : ,were
R5067 T8028 T8026 attr Pax6,were
R5068 T8029 T8028 punct -,Pax6
R5069 T8030 T8031 compound F,CTG
R5070 T8031 T8028 appos CTG,Pax6
R5071 T8032 T8031 compound CAG,CTG
R5072 T8033 T8031 compound CCA,CTG
R5073 T8034 T8031 compound AAA,CTG
R5074 T8035 T8031 compound TAG,CTG
R5075 T8036 T8031 compound ATC,CTG
R5076 T8037 T8031 compound TAC,CTG
R5077 T8038 T8028 punct ;,Pax6
R5078 T8039 T8028 appos Pax6,Pax6
R5079 T8040 T8039 punct -,Pax6
R5080 T8041 T8042 compound R,TT
R5081 T8042 T8039 appos TT,Pax6
R5082 T8043 T8042 compound CGA,TT
R5083 T8044 T8042 compound TCA,TT
R5084 T8045 T8042 compound CAT,TT
R5085 T8046 T8042 compound GCT,TT
R5086 T8047 T8042 compound CTC,TT
R5087 T8048 T8042 compound TCC,TT
R5088 T8049 T8028 punct ;,Pax6
R5089 T8050 T8028 appos Homer3,Pax6
R5090 T8051 T8050 punct -,Homer3
R5091 T8052 T8053 compound F,AGC
R5092 T8053 T8050 appos AGC,Homer3
R5093 T8054 T8053 compound CCC,AGC
R5094 T8055 T8053 compound AGG,AGC
R5095 T8056 T8053 compound TGG,AGC
R5096 T8057 T8053 compound CTG,AGC
R5097 T8058 T8053 compound TAG,AGC
R5098 T8059 T8028 punct ;,Pax6
R5099 T8060 T8028 appos Homer3,Pax6
R5100 T8061 T8060 punct -,Homer3
R5101 T8062 T8063 compound R,G
R5102 T8063 T8060 appos G,Homer3
R5103 T8064 T8063 compound CTC,G
R5104 T8065 T8063 compound TAC,G
R5105 T8066 T8063 compound ACA,G
R5106 T8067 T8063 compound GTG,G
R5107 T8068 T8063 compound CAA,G
R5108 T8069 T8063 compound AGC,G
R5109 T8070 T8063 compound TCA,G
R5110 T8071 T8028 punct ;,Pax6
R5111 T8072 T8028 appos Trim11,Pax6
R5112 T8073 T8072 punct -,Trim11
R5113 T8074 T8075 compound F,CTG
R5114 T8075 T8072 appos CTG,Trim11
R5115 T8076 T8075 compound GTG,CTG
R5116 T8077 T8075 compound CAG,CTG
R5117 T8078 T8075 compound GAT,CTG
R5118 T8079 T8075 compound GTG,CTG
R5119 T8080 T8075 compound AAG,CTG
R5120 T8081 T8028 punct ;,Pax6
R5121 T8082 T8028 appos Trim11,Pax6
R5122 T8083 T8082 punct -,Trim11
R5123 T8084 T8085 compound R,GG
R5124 T8085 T8082 appos GG,Trim11
R5125 T8086 T8085 compound GCC,GG
R5126 T8087 T8085 compound TGC,GG
R5127 T8088 T8085 compound AGA,GG
R5128 T8089 T8085 compound TAG,GG
R5129 T8090 T8085 compound TCA,GG
R5130 T8091 T8085 compound TAG,GG
R5131 T8092 T8028 punct ;,Pax6
R5132 T8093 T8028 appos Dncl1,Pax6
R5133 T8094 T8093 punct -,Dncl1
R5134 T8095 T8096 compound F,G
R5135 T8096 T8093 appos G,Dncl1
R5136 T8097 T8096 compound CAA,G
R5137 T8098 T8096 compound AAA,G
R5138 T8099 T8096 compound TGC,G
R5139 T8100 T8096 compound AGA,G
R5140 T8101 T8096 compound CAT,G
R5141 T8102 T8096 compound GTC,G
R5142 T8103 T8028 punct ;,Pax6
R5143 T8104 T8028 appos Dncl1,Pax6
R5144 T8105 T8104 punct -,Dncl1
R5145 T8106 T8107 compound R,GGG
R5146 T8107 T8104 appos GGG,Dncl1
R5147 T8108 T8107 compound CTA,GGG
R5148 T8109 T8107 compound AGG,GGG
R5149 T8110 T8107 compound GAG,GGG
R5150 T8111 T8107 compound AAA,GGG
R5151 T8112 T8107 compound AAA,GGG
R5152 T8113 T8107 compound ATG,GGG
R5153 T8114 T8028 punct ;,Pax6
R5154 T8115 T8116 compound Gapdh,F
R5155 T8116 T8028 appos F,Pax6
R5156 T8117 T8116 punct -,F
R5157 T8118 T8116 punct : ,F
R5158 T8119 T8120 compound CAT,GC
R5159 T8120 T8116 appos GC,F
R5160 T8121 T8120 compound CAC,GC
R5161 T8122 T8120 compound CAT,GC
R5162 T8123 T8120 compound CTT,GC
R5163 T8124 T8120 compound CCA,GC
R5164 T8125 T8120 compound GGA,GC
R5165 T8126 T8028 punct ;,Pax6
R5166 T8127 T8128 compound Gapdh,R
R5167 T8128 T8028 appos R,Pax6
R5168 T8129 T8128 punct -,R
R5169 T8130 T8128 punct : ,R
R5170 T8131 T8132 compound ATG,TT
R5171 T8132 T8128 appos TT,R
R5172 T8133 T8132 compound ACC,TT
R5173 T8134 T8132 compound TTG,TT
R5174 T8135 T8132 compound CCC,TT
R5175 T8136 T8132 compound ACA,TT
R5176 T8137 T8132 compound GCC,TT
R5177 T8138 T8028 punct ;,Pax6
R5178 T8139 T8140 compound Atp5a1,F
R5179 T8140 T8028 appos F,Pax6
R5180 T8141 T8140 punct -,F
R5181 T8142 T8140 punct : ,F
R5182 T8143 T8144 compound CAC,CA
R5183 T8144 T8140 appos CA,F
R5184 T8145 T8144 compound ACG,CA
R5185 T8146 T8144 compound TGA,CA
R5186 T8147 T8144 compound GAT,CA
R5187 T8148 T8144 compound GTC,CA
R5188 T8149 T8144 compound CTC,CA
R5189 T8150 T8028 punct ;,Pax6
R5190 T8151 T8152 compound Atp5a1,R
R5191 T8152 T8028 appos R,Pax6
R5192 T8153 T8152 punct -,R
R5193 T8154 T8152 punct : ,R
R5194 T8155 T8156 compound CAC,A
R5195 T8156 T8152 appos A,R
R5196 T8157 T8156 compound AGA,A
R5197 T8158 T8156 compound GAT,A
R5198 T8159 T8156 compound TCG,A
R5199 T8160 T8156 compound GGG,A
R5200 T8161 T8156 compound ATA,A
R5201 T8162 T8026 punct .,were
R5202 T8164 T8165 nummod 10,ng
R5203 T8165 T8166 compound ng,cDNA
R5204 T8166 T8168 nsubjpass cDNA,amplified
R5205 T8167 T8166 compound library,cDNA
R5206 T8169 T8168 auxpass were,amplified
R5207 T8170 T8168 prep in,amplified
R5208 T8171 T8172 det a,reaction
R5209 T8172 T8170 pobj reaction,in
R5210 T8173 T8172 acl containing,reaction
R5211 T8174 T8175 nummod 1,buffer
R5212 T8175 T8173 dobj buffer,containing
R5213 T8176 T8174 punct x,1
R5214 T8177 T8175 compound AmpliTaq,buffer
R5215 T8178 T8175 compound polymerase,buffer
R5216 T8179 T8180 punct (,Elmer
R5217 T8180 T8175 parataxis Elmer,buffer
R5218 T8181 T8180 compound Perkin,Elmer
R5219 T8182 T8180 punct ),Elmer
R5220 T8183 T8175 punct ", ",buffer
R5221 T8184 T8185 nummod 1.5,mM
R5222 T8185 T8186 compound mM,MgCl2
R5223 T8186 T8175 conj MgCl2,buffer
R5224 T8187 T8186 punct ", ",MgCl2
R5225 T8188 T8189 nmod 200μM,primer
R5226 T8189 T8186 conj primer,MgCl2
R5227 T8190 T8189 det each,primer
R5228 T8191 T8189 cc and,primer
R5229 T8192 T8193 nummod 2.5,units
R5230 T8193 T8189 conj units,primer
R5231 T8194 T8193 prep of,units
R5232 T8195 T8196 compound AmpliTaq,polymerase
R5233 T8196 T8194 pobj polymerase,of
R5234 T8197 T8198 punct (,Elmer
R5235 T8198 T8196 parataxis Elmer,polymerase
R5236 T8199 T8198 compound Perkin,Elmer
R5237 T8200 T8198 punct ),Elmer
R5238 T8201 T8168 punct .,amplified
R5239 T8203 T8204 compound PCR,conditions
R5240 T8204 T8205 nsubj conditions,were
R5241 T8206 T8205 punct (,were
R5242 T8207 T8208 nummod 95,°C
R5243 T8208 T8205 attr °C,were
R5244 T8209 T8208 prep for,°C
R5245 T8210 T8211 nummod 30,sec
R5246 T8211 T8209 pobj sec,for
R5247 T8212 T8208 punct ),°C
R5248 T8213 T8208 punct ×,°C
R5249 T8214 T8208 appos 1,°C
R5250 T8215 T8208 punct ", ",°C
R5251 T8216 T8217 punct (,°C
R5252 T8217 T8208 conj °C,°C
R5253 T8218 T8217 nummod 94,°C
R5254 T8219 T8217 prep for,°C
R5255 T8220 T8221 nummod 30,sec
R5256 T8221 T8219 pobj sec,for
R5257 T8222 T8217 punct ", ",°C
R5258 T8223 T8224 nummod 55,°C
R5259 T8224 T8217 appos °C,°C
R5260 T8225 T8224 prep for,°C
R5261 T8226 T8227 nummod 30,sec
R5262 T8227 T8225 pobj sec,for
R5263 T8228 T8217 punct ", ",°C
R5264 T8229 T8230 nummod 72,°C
R5265 T8230 T8217 appos °C,°C
R5266 T8231 T8230 prep for,°C
R5267 T8232 T8233 nummod 30,sec
R5268 T8233 T8231 pobj sec,for
R5269 T8234 T8217 punct ),°C
R5270 T8235 T8217 punct ×,°C
R5271 T8236 T8217 appos 32,°C
R5272 T8237 T8217 cc and,°C
R5273 T8238 T8239 punct (,°C
R5274 T8239 T8217 conj °C,°C
R5275 T8240 T8239 nummod 72,°C
R5276 T8241 T8239 prep for,°C
R5277 T8242 T8243 nummod 2,min
R5278 T8243 T8241 pobj min,for
R5279 T8244 T8239 punct ),°C
R5280 T8245 T8239 punct ×,°C
R5281 T8246 T8239 appos 1,°C
R5282 T8247 T8205 punct .,were
R5283 T8249 T8250 nsubjpass Products,resolved
R5284 T8251 T8250 auxpass were,resolved
R5285 T8252 T8250 prep on,resolved
R5286 T8253 T8254 det a,gel
R5287 T8254 T8252 pobj gel,on
R5288 T8255 T8256 nummod 2.5,%
R5289 T8256 T8254 compound %,gel
R5290 T8257 T8254 compound agarose,gel
R5291 T8258 T8250 prep with,resolved
R5292 T8259 T8260 compound ΦX174,HaeIII
R5293 T8260 T8262 compound HaeIII,markers
R5294 T8261 T8260 punct /,HaeIII
R5295 T8262 T8258 pobj markers,with
R5296 T8263 T8262 compound size,markers
R5297 T8264 T8265 punct (,Promega
R5298 T8265 T8262 parataxis Promega,markers
R5299 T8266 T8265 punct ),Promega
R5300 T8267 T8250 punct .,resolved

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T7961 1149-1156 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes markers
T7960 1131-1136 NCBITaxon:1910954 denotes ΦX174
T7959 1114-1121 CHEBI:2511 denotes agarose
T7958 928-938 GO_EXT:polymerase denotes polymerase
T7957 895-901 SO_EXT:0000112 denotes primer
T7956 877-882 CHEBI:6636 denotes MgCl2
T7955 847-853 CHEBI_CHMO_EXT:buffer_solution denotes buffer
T7954 836-846 GO_EXT:polymerase denotes polymerase
T7953 780-784 SO_EXT:cDNA denotes cDNA
T7952 736-737 SO:0001031 denotes R
T7951 729-735 PR_EXT:000004453 denotes Atp5a1
T7950 698-699 SO:0001030 denotes F
T7949 691-697 PR_EXT:000004453 denotes Atp5a1
T7948 660-661 SO:0001031 denotes R
T7947 654-659 GO_PR_EXT:glyceraldehyde_3_phosphate_dehydrogenase denotes Gapdh
T7946 623-624 SO:0001030 denotes F
T7945 617-622 GO_PR_EXT:glyceraldehyde_3_phosphate_dehydrogenase denotes Gapdh
T7944 586-587 SO:0001031 denotes R
T7943 580-585 PR_EXT:000002325 denotes Dncl1
T7942 551-552 SO:0001030 denotes F
T7941 545-550 PR_EXT:000002325 denotes Dncl1
T7940 515-516 SO:0001031 denotes R
T7939 508-514 PR_EXT:000016642 denotes Trim11
T7938 481-482 SO:0001030 denotes F
T7937 474-480 PR_EXT:000016642 denotes Trim11
T7936 441-442 SO:0001031 denotes R
T7935 434-440 PR_EXT:000008680 denotes Homer3
T7934 407-408 SO:0001030 denotes F
T7933 400-406 PR_EXT:000008680 denotes Homer3
T7932 370-371 SO:0001031 denotes R
T7931 365-369 PR_EXT:000012318 denotes Pax6
T7930 334-335 SO:0001030 denotes F
T7929 329-333 PR_EXT:000012318 denotes Pax6
T7928 313-322 SO_EXT:biological_sequence denotes sequences
T7927 306-312 SO_EXT:0000112 denotes Primer
T7926 283-289 SO_EXT:sequence_cloned_entity denotes clones
T7925 275-282 GO:0008380 denotes spliced
T7924 244-250 SO_EXT:0000188 denotes intron
T7923 200-207 SO_EXT:0000112 denotes Primers
T7922 184-190 PR_EXT:000004453 denotes Atp5a1
T7921 174-179 GO_PR_EXT:glyceraldehyde_3_phosphate_dehydrogenase denotes Gapdh
T7920 168-173 SO_EXT:0000704 denotes genes
T7918 128-134 PR_EXT:000016642 denotes Trim11
T7917 121-126 PR_EXT:000002325 denotes Dncl1
T7916 113-119 PR_EXT:000008680 denotes Homer3
T7919 158-167 GO:0010467 denotes expressed
T7915 107-111 PR_EXT:000012318 denotes Pax6
T7914 54-58 SO_EXT:cDNA denotes cDNA
T7913 40-46 SO_EXT:sequence_cloned_entity denotes clones

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T7912 1131-1136 NCBITaxon:1910954 denotes ΦX174
T7911 1114-1121 CHEBI:2511 denotes agarose
T7910 895-901 SO:0000112 denotes primer
T7909 877-882 CHEBI:6636 denotes MgCl2
T7908 736-737 SO:0001031 denotes R
T7907 729-735 PR:000004453 denotes Atp5a1
T7906 698-699 SO:0001030 denotes F
T7905 691-697 PR:000004453 denotes Atp5a1
T7904 660-661 SO:0001031 denotes R
T7903 623-624 SO:0001030 denotes F
T7902 586-587 SO:0001031 denotes R
T7901 580-585 PR:000002325 denotes Dncl1
T7900 551-552 SO:0001030 denotes F
T7899 545-550 PR:000002325 denotes Dncl1
T7898 515-516 SO:0001031 denotes R
T7897 508-514 PR:000016642 denotes Trim11
T7896 481-482 SO:0001030 denotes F
T7895 474-480 PR:000016642 denotes Trim11
T7894 441-442 SO:0001031 denotes R
T7893 434-440 PR:000008680 denotes Homer3
T7892 407-408 SO:0001030 denotes F
T7891 400-406 PR:000008680 denotes Homer3
T7890 370-371 SO:0001031 denotes R
T7889 365-369 PR:000012318 denotes Pax6
T7888 334-335 SO:0001030 denotes F
T7887 329-333 PR:000012318 denotes Pax6
T7886 306-312 SO:0000112 denotes Primer
T7885 275-282 GO:0008380 denotes spliced
T7884 244-250 SO:0000188 denotes intron
T7883 200-207 SO:0000112 denotes Primers
T7882 184-190 PR:000004453 denotes Atp5a1
T7881 168-173 SO:0000704 denotes genes
T7880 158-167 GO:0010467 denotes expressed
T7879 128-134 PR:000016642 denotes Trim11
T7878 121-126 PR:000002325 denotes Dncl1
T7877 113-119 PR:000008680 denotes Homer3
T7876 107-111 PR:000012318 denotes Pax6