PMC:1208879 / 29123-29854
Annnotations
craft-sa-dev
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T7843 | 730-731 | . | denotes | . |
T7842 | 710-720 | NN | denotes | expression |
T7841 | 702-703 | HYPH | denotes | - |
T7840 | 703-709 | NN | denotes | length |
T7839 | 698-702 | JJ | denotes | full |
T7838 | 721-730 | NN | denotes | construct |
T7837 | 696-697 | DT | denotes | a |
T7836 | 689-695 | VB | denotes | create |
T7835 | 686-688 | TO | denotes | to |
T7834 | 670-671 | HYPH | denotes | - |
T7833 | 671-677 | NN | denotes | Homer3 |
T7832 | 665-670 | NN | denotes | pPC86 |
T7831 | 678-685 | NN | denotes | plasmid |
T7830 | 661-664 | DT | denotes | the |
T7829 | 656-660 | IN | denotes | into |
T7828 | 647-655 | VBN | denotes | inserted |
T7827 | 643-646 | CC | denotes | and |
T7826 | 639-642 | NN | denotes | PCR |
T7825 | 636-638 | IN | denotes | by |
T7824 | 624-629 | NN | denotes | IMAGE |
T7823 | 630-635 | NN | denotes | clone |
T7822 | 620-623 | DT | denotes | the |
T7821 | 615-619 | IN | denotes | from |
T7820 | 601-604 | VBD | denotes | was |
T7819 | 605-614 | VBN | denotes | amplified |
T7818 | 584-591 | JJ | denotes | missing |
T7817 | 592-600 | NN | denotes | fragment |
T7816 | 580-583 | DT | denotes | The |
T7815 | 579-731 | sentence | denotes | The missing fragment was amplified from the IMAGE clone by PCR and inserted into the pPC86-Homer3 plasmid to create a full-length expression construct. |
T7814 | 578-579 | . | denotes | . |
T7813 | 570-578 | NNP | denotes | Research |
T7812 | 561-569 | NNP | denotes | Genomics |
T7811 | 557-560 | IN | denotes | for |
T7810 | 541-549 | NNP | denotes | Franklin |
T7809 | 532-540 | NNP | denotes | Rosalind |
T7808 | 550-556 | NNP | denotes | Centre |
T7807 | 528-531 | DT | denotes | the |
T7806 | 523-527 | IN | denotes | from |
T7805 | 514-522 | VBN | denotes | obtained |
T7804 | 510-513 | CC | denotes | and |
T7803 | 492-500 | NN | denotes | sequence |
T7802 | 481-491 | NN | denotes | nucleotide |
T7801 | 477-480 | NN | denotes | EST |
T7800 | 501-509 | NN | denotes | database |
T7799 | 473-476 | DT | denotes | the |
T7798 | 470-472 | IN | denotes | of |
T7797 | 457-462 | NNP | denotes | BLAST |
T7796 | 463-469 | NN | denotes | search |
T7795 | 455-456 | DT | denotes | a |
T7794 | 452-454 | IN | denotes | by |
T7793 | 437-440 | VBD | denotes | was |
T7792 | 425-430 | NN | denotes | amino |
T7791 | 422-424 | CD | denotes | 70 |
T7790 | 412-413 | HYPH | denotes | - |
T7789 | 413-421 | JJ | denotes | terminal |
T7788 | 411-412 | NN | denotes | N |
T7787 | 403-410 | JJ | denotes | missing |
T7786 | 431-436 | NNS | denotes | acids |
T7785 | 399-402 | DT | denotes | the |
T7784 | 388-398 | VBG | denotes | containing |
T7783 | 386-387 | -RRB- | denotes | ) |
T7782 | 371-377 | NN | denotes | number |
T7781 | 378-386 | NN | denotes | BE569374 |
T7780 | 361-370 | NN | denotes | accession |
T7779 | 359-361 | , | denotes | , |
T7778 | 352-359 | CD | denotes | 3602414 |
T7777 | 340-345 | NN | denotes | IMAGE |
T7776 | 346-351 | NN | denotes | clone |
T7775 | 339-340 | -LRB- | denotes | ( |
T7774 | 328-332 | NN | denotes | cDNA |
T7773 | 333-338 | NN | denotes | clone |
T7772 | 326-327 | DT | denotes | a |
T7771 | 324-326 | , | denotes | , |
T7770 | 319-324 | NNS | denotes | tests |
T7769 | 310-318 | JJ | denotes | pairwise |
T7768 | 306-309 | IN | denotes | for |
T7767 | 293-299 | NN | denotes | Homer3 |
T7766 | 285-286 | HYPH | denotes | - |
T7765 | 286-292 | NN | denotes | length |
T7764 | 281-285 | JJ | denotes | full |
T7763 | 300-305 | NN | denotes | clone |
T7762 | 279-280 | DT | denotes | a |
T7761 | 441-451 | VBN | denotes | identified |
T7760 | 272-278 | VB | denotes | create |
T7759 | 269-271 | TO | denotes | To |
T7758 | 268-579 | sentence | denotes | To create a full-length Homer3 clone for pairwise tests, a cDNA clone (IMAGE clone 3602414, accession number BE569374) containing the missing N-terminal 70 amino acids was identified by a BLAST search of the EST nucleotide sequence database and obtained from the Rosalind Franklin Centre for Genomics Research. |
T7757 | 267-268 | . | denotes | . |
T7756 | 261-267 | RB | denotes | before |
T7755 | 258-260 | IN | denotes | as |
T7754 | 243-247 | NN | denotes | LacZ |
T7753 | 239-242 | CC | denotes | and |
T7752 | 234-238 | NN | denotes | URA3 |
T7751 | 232-234 | , | denotes | , |
T7750 | 228-232 | NN | denotes | HIS3 |
T7749 | 248-257 | NNS | denotes | reporters |
T7748 | 224-227 | DT | denotes | the |
T7747 | 221-223 | IN | denotes | of |
T7746 | 210-220 | NN | denotes | activation |
T7745 | 206-209 | IN | denotes | for |
T7744 | 187-196 | VBG | denotes | resulting |
T7743 | 197-205 | NNS | denotes | colonies |
T7742 | 183-186 | DT | denotes | the |
T7741 | 175-182 | VBG | denotes | testing |
T7740 | 171-174 | CC | denotes | and |
T7739 | 169-170 | -RRB- | denotes | ) |
T7738 | 164-169 | NN | denotes | pPC86 |
T7737 | 161-163 | IN | denotes | in |
T7736 | 160-161 | -LRB- | denotes | ( |
T7735 | 150-159 | NN | denotes | construct |
T7734 | 145-149 | NN | denotes | prey |
T7733 | 141-144 | CD | denotes | one |
T7732 | 137-140 | CC | denotes | and |
T7731 | 135-136 | -RRB- | denotes | ) |
T7730 | 129-135 | NN | denotes | pDBLeu |
T7729 | 126-128 | IN | denotes | in |
T7728 | 125-126 | -LRB- | denotes | ( |
T7727 | 115-124 | NN | denotes | construct |
T7726 | 110-114 | NN | denotes | bait |
T7725 | 106-109 | CD | denotes | one |
T7724 | 101-105 | IN | denotes | with |
T7723 | 89-94 | NN | denotes | yeast |
T7722 | 82-88 | NN | denotes | MaV203 |
T7721 | 95-100 | NNS | denotes | cells |
T7720 | 72-81 | JJ | denotes | competent |
T7719 | 59-71 | VBG | denotes | transforming |
T7718 | 56-58 | IN | denotes | by |
T7717 | 44-48 | VBD | denotes | were |
T7716 | 49-55 | VBN | denotes | tested |
T7715 | 31-43 | NNS | denotes | interactions |
T7714 | 22-30 | JJ | denotes | Specific |
T7713 | 21-268 | sentence | denotes | Specific interactions were tested by transforming competent MaV203 yeast cells with one bait construct (in pDBLeu) and one prey construct (in pPC86) and testing the resulting colonies for activation of the HIS3, URA3 and LacZ reporters as before. |
T7712 | 9-21 | NNS | denotes | interactions |
T7711 | 0-8 | JJ | denotes | Pairwise |
R4882 | T7711 | T7712 | amod | Pairwise,interactions |
R4883 | T7714 | T7715 | amod | Specific,interactions |
R4884 | T7715 | T7716 | nsubjpass | interactions,tested |
R4885 | T7717 | T7716 | auxpass | were,tested |
R4886 | T7718 | T7716 | prep | by,tested |
R4887 | T7719 | T7718 | pcomp | transforming,by |
R4888 | T7720 | T7721 | amod | competent,cells |
R4889 | T7721 | T7719 | dobj | cells,transforming |
R4890 | T7722 | T7721 | compound | MaV203,cells |
R4891 | T7723 | T7721 | compound | yeast,cells |
R4892 | T7724 | T7721 | prep | with,cells |
R4893 | T7725 | T7726 | nummod | one,bait |
R4894 | T7726 | T7727 | compound | bait,construct |
R4895 | T7727 | T7724 | pobj | construct,with |
R4896 | T7728 | T7727 | punct | (,construct |
R4897 | T7729 | T7727 | prep | in,construct |
R4898 | T7730 | T7729 | pobj | pDBLeu,in |
R4899 | T7731 | T7727 | punct | ),construct |
R4900 | T7732 | T7727 | cc | and,construct |
R4901 | T7733 | T7734 | nummod | one,prey |
R4902 | T7734 | T7735 | compound | prey,construct |
R4903 | T7735 | T7727 | conj | construct,construct |
R4904 | T7736 | T7735 | punct | (,construct |
R4905 | T7737 | T7735 | prep | in,construct |
R4906 | T7738 | T7737 | pobj | pPC86,in |
R4907 | T7739 | T7719 | punct | ),transforming |
R4908 | T7740 | T7719 | cc | and,transforming |
R4909 | T7741 | T7719 | conj | testing,transforming |
R4910 | T7742 | T7743 | det | the,colonies |
R4911 | T7743 | T7741 | dobj | colonies,testing |
R4912 | T7744 | T7743 | amod | resulting,colonies |
R4913 | T7745 | T7741 | prep | for,testing |
R4914 | T7746 | T7745 | pobj | activation,for |
R4915 | T7747 | T7746 | prep | of,activation |
R4916 | T7748 | T7749 | det | the,reporters |
R4917 | T7749 | T7747 | pobj | reporters,of |
R4918 | T7750 | T7749 | nmod | HIS3,reporters |
R4919 | T7751 | T7750 | punct | ", ",HIS3 |
R4920 | T7752 | T7750 | conj | URA3,HIS3 |
R4921 | T7753 | T7752 | cc | and,URA3 |
R4922 | T7754 | T7752 | conj | LacZ,URA3 |
R4923 | T7755 | T7741 | prep | as,testing |
R4924 | T7756 | T7755 | pcomp | before,as |
R4925 | T7757 | T7716 | punct | .,tested |
R4926 | T7759 | T7760 | aux | To,create |
R4927 | T7760 | T7761 | advcl | create,identified |
R4928 | T7762 | T7763 | det | a,clone |
R4929 | T7763 | T7760 | dobj | clone,create |
R4930 | T7764 | T7765 | amod | full,length |
R4931 | T7765 | T7763 | compound | length,clone |
R4932 | T7766 | T7765 | punct | -,length |
R4933 | T7767 | T7763 | compound | Homer3,clone |
R4934 | T7768 | T7760 | prep | for,create |
R4935 | T7769 | T7770 | amod | pairwise,tests |
R4936 | T7770 | T7768 | pobj | tests,for |
R4937 | T7771 | T7761 | punct | ", ",identified |
R4938 | T7772 | T7773 | det | a,clone |
R4939 | T7773 | T7761 | nsubjpass | clone,identified |
R4940 | T7774 | T7773 | compound | cDNA,clone |
R4941 | T7775 | T7776 | punct | (,clone |
R4942 | T7776 | T7773 | parataxis | clone,clone |
R4943 | T7777 | T7776 | compound | IMAGE,clone |
R4944 | T7778 | T7776 | nummod | 3602414,clone |
R4945 | T7779 | T7776 | punct | ", ",clone |
R4946 | T7780 | T7781 | compound | accession,BE569374 |
R4947 | T7781 | T7776 | appos | BE569374,clone |
R4948 | T7782 | T7781 | compound | number,BE569374 |
R4949 | T7783 | T7776 | punct | ),clone |
R4950 | T7784 | T7773 | acl | containing,clone |
R4951 | T7785 | T7786 | det | the,acids |
R4952 | T7786 | T7784 | dobj | acids,containing |
R4953 | T7787 | T7786 | amod | missing,acids |
R4954 | T7788 | T7789 | npadvmod | N,terminal |
R4955 | T7789 | T7786 | amod | terminal,acids |
R4956 | T7790 | T7789 | punct | -,terminal |
R4957 | T7791 | T7786 | nummod | 70,acids |
R4958 | T7792 | T7786 | compound | amino,acids |
R4959 | T7793 | T7761 | auxpass | was,identified |
R4960 | T7794 | T7761 | prep | by,identified |
R4961 | T7795 | T7796 | det | a,search |
R4962 | T7796 | T7794 | pobj | search,by |
R4963 | T7797 | T7796 | compound | BLAST,search |
R4964 | T7798 | T7796 | prep | of,search |
R4965 | T7799 | T7800 | det | the,database |
R4966 | T7800 | T7798 | pobj | database,of |
R4967 | T7801 | T7800 | compound | EST,database |
R4968 | T7802 | T7803 | compound | nucleotide,sequence |
R4969 | T7803 | T7800 | compound | sequence,database |
R4970 | T7804 | T7761 | cc | and,identified |
R4971 | T7805 | T7761 | conj | obtained,identified |
R4972 | T7806 | T7805 | prep | from,obtained |
R4973 | T7807 | T7808 | det | the,Centre |
R4974 | T7808 | T7806 | pobj | Centre,from |
R4975 | T7809 | T7810 | compound | Rosalind,Franklin |
R4976 | T7810 | T7808 | compound | Franklin,Centre |
R4977 | T7811 | T7808 | prep | for,Centre |
R4978 | T7812 | T7813 | compound | Genomics,Research |
R4979 | T7813 | T7811 | pobj | Research,for |
R4980 | T7814 | T7761 | punct | .,identified |
R4981 | T7816 | T7817 | det | The,fragment |
R4982 | T7817 | T7819 | nsubj | fragment,amplified |
R4983 | T7818 | T7817 | amod | missing,fragment |
R4984 | T7820 | T7819 | aux | was,amplified |
R4985 | T7821 | T7819 | prep | from,amplified |
R4986 | T7822 | T7823 | det | the,clone |
R4987 | T7823 | T7821 | pobj | clone,from |
R4988 | T7824 | T7823 | compound | IMAGE,clone |
R4989 | T7825 | T7819 | prep | by,amplified |
R4990 | T7826 | T7825 | pobj | PCR,by |
R4991 | T7827 | T7819 | cc | and,amplified |
R4992 | T7828 | T7819 | conj | inserted,amplified |
R4993 | T7829 | T7828 | prep | into,inserted |
R4994 | T7830 | T7831 | det | the,plasmid |
R4995 | T7831 | T7829 | pobj | plasmid,into |
R4996 | T7832 | T7833 | compound | pPC86,Homer3 |
R4997 | T7833 | T7831 | compound | Homer3,plasmid |
R4998 | T7834 | T7833 | punct | -,Homer3 |
R4999 | T7835 | T7836 | aux | to,create |
R5000 | T7836 | T7819 | advcl | create,amplified |
R5001 | T7837 | T7838 | det | a,construct |
R5002 | T7838 | T7836 | dobj | construct,create |
R5003 | T7839 | T7840 | amod | full,length |
R5004 | T7840 | T7838 | compound | length,construct |
R5005 | T7841 | T7840 | punct | -,length |
R5006 | T7842 | T7838 | compound | expression,construct |
R5007 | T7843 | T7819 | punct | .,amplified |
craft-ca-core-ex-dev
Below, discontinuous spans are shown in the chain model. You can change it to the bag model.
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T7710 | 721-730 | SO_EXT:engineered_biological_sequence | denotes | construct |
T7709 | 710-720 | GO:0010467 | denotes | expression |
T7708 | 678-685 | SO_EXT:0000155 | denotes | plasmid |
T7707 | 671-677 | PR_EXT:000008680 | denotes | Homer3 |
T7706 | 647-655 | SO_EXT:sequence_insertion_process | denotes | inserted |
T7705 | 630-635 | SO_EXT:sequence_cloned_entity | denotes | clone |
T7704 | 492-500 | SO_EXT:biological_sequence | denotes | sequence |
T7703 | 481-491 | CHEBI_SO_EXT:nucleotide | denotes | nucleotide |
T7702 | 477-480 | SO_EXT:0000345 | denotes | EST |
T7701 | 425-436 | CHEBI_SO_EXT:amino_acid | denotes | amino acids |
T7700 | 411-421 | CHEBI_SO_EXT:N_terminus_or_N_terminal_region | denotes | N-terminal |
T7699 | 346-351 | SO_EXT:sequence_cloned_entity | denotes | clone |
T7698 | 328-338 | SO_EXT:0000317 | denotes | cDNA clone |
T7697 | 300-305 | SO_EXT:sequence_cloned_entity | denotes | clone |
T7696 | 293-299 | PR_EXT:000008680 | denotes | Homer3 |
T7695 | 243-247 | PR_EXT:000033987 | denotes | LacZ |
T7694 | 234-238 | PR_EXT:P03962 | denotes | URA3 |
T7693 | 228-232 | PR_EXT:P06633 | denotes | HIS3 |
T7692 | 150-159 | SO_EXT:engineered_biological_sequence | denotes | construct |
T7691 | 115-124 | SO_EXT:engineered_biological_sequence | denotes | construct |
T7690 | 95-100 | CL_GO_EXT:cell | denotes | cells |
T7689 | 89-94 | NCBITaxon_EXT:yeast | denotes | yeast |
T7688 | 59-71 | GO:0009294 | denotes | transforming |
craft-ca-core-dev
Below, discontinuous spans are shown in the chain model. You can change it to the bag model.
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T7687 | 710-720 | GO:0010467 | denotes | expression |
T7686 | 678-685 | SO:0000155 | denotes | plasmid |
T7685 | 671-677 | PR:000008680 | denotes | Homer3 |
T7684 | 477-480 | SO:0000345 | denotes | EST |
T7683 | 328-338 | SO:0000317 | denotes | cDNA clone |
T7682 | 293-299 | PR:000008680 | denotes | Homer3 |
T7681 | 243-247 | PR:000033987 | denotes | LacZ |
T7680 | 234-238 | PR:P03962 | denotes | URA3 |
T7679 | 228-232 | PR:P06633 | denotes | HIS3 |
T7678 | 59-71 | GO:0009294 | denotes | transforming |