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PMC:1208879 / 29123-29854 JSONTXT

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Id Subject Object Predicate Lexical cue
T7843 730-731 . denotes .
T7842 710-720 NN denotes expression
T7841 702-703 HYPH denotes -
T7840 703-709 NN denotes length
T7839 698-702 JJ denotes full
T7838 721-730 NN denotes construct
T7837 696-697 DT denotes a
T7836 689-695 VB denotes create
T7835 686-688 TO denotes to
T7834 670-671 HYPH denotes -
T7833 671-677 NN denotes Homer3
T7832 665-670 NN denotes pPC86
T7831 678-685 NN denotes plasmid
T7830 661-664 DT denotes the
T7829 656-660 IN denotes into
T7828 647-655 VBN denotes inserted
T7827 643-646 CC denotes and
T7826 639-642 NN denotes PCR
T7825 636-638 IN denotes by
T7824 624-629 NN denotes IMAGE
T7823 630-635 NN denotes clone
T7822 620-623 DT denotes the
T7821 615-619 IN denotes from
T7820 601-604 VBD denotes was
T7819 605-614 VBN denotes amplified
T7818 584-591 JJ denotes missing
T7817 592-600 NN denotes fragment
T7816 580-583 DT denotes The
T7815 579-731 sentence denotes The missing fragment was amplified from the IMAGE clone by PCR and inserted into the pPC86-Homer3 plasmid to create a full-length expression construct.
T7814 578-579 . denotes .
T7813 570-578 NNP denotes Research
T7812 561-569 NNP denotes Genomics
T7811 557-560 IN denotes for
T7810 541-549 NNP denotes Franklin
T7809 532-540 NNP denotes Rosalind
T7808 550-556 NNP denotes Centre
T7807 528-531 DT denotes the
T7806 523-527 IN denotes from
T7805 514-522 VBN denotes obtained
T7804 510-513 CC denotes and
T7803 492-500 NN denotes sequence
T7802 481-491 NN denotes nucleotide
T7801 477-480 NN denotes EST
T7800 501-509 NN denotes database
T7799 473-476 DT denotes the
T7798 470-472 IN denotes of
T7797 457-462 NNP denotes BLAST
T7796 463-469 NN denotes search
T7795 455-456 DT denotes a
T7794 452-454 IN denotes by
T7793 437-440 VBD denotes was
T7792 425-430 NN denotes amino
T7791 422-424 CD denotes 70
T7790 412-413 HYPH denotes -
T7789 413-421 JJ denotes terminal
T7788 411-412 NN denotes N
T7787 403-410 JJ denotes missing
T7786 431-436 NNS denotes acids
T7785 399-402 DT denotes the
T7784 388-398 VBG denotes containing
T7783 386-387 -RRB- denotes )
T7782 371-377 NN denotes number
T7781 378-386 NN denotes BE569374
T7780 361-370 NN denotes accession
T7779 359-361 , denotes ,
T7778 352-359 CD denotes 3602414
T7777 340-345 NN denotes IMAGE
T7776 346-351 NN denotes clone
T7775 339-340 -LRB- denotes (
T7774 328-332 NN denotes cDNA
T7773 333-338 NN denotes clone
T7772 326-327 DT denotes a
T7771 324-326 , denotes ,
T7770 319-324 NNS denotes tests
T7769 310-318 JJ denotes pairwise
T7768 306-309 IN denotes for
T7767 293-299 NN denotes Homer3
T7766 285-286 HYPH denotes -
T7765 286-292 NN denotes length
T7764 281-285 JJ denotes full
T7763 300-305 NN denotes clone
T7762 279-280 DT denotes a
T7761 441-451 VBN denotes identified
T7760 272-278 VB denotes create
T7759 269-271 TO denotes To
T7758 268-579 sentence denotes To create a full-length Homer3 clone for pairwise tests, a cDNA clone (IMAGE clone 3602414, accession number BE569374) containing the missing N-terminal 70 amino acids was identified by a BLAST search of the EST nucleotide sequence database and obtained from the Rosalind Franklin Centre for Genomics Research.
T7757 267-268 . denotes .
T7756 261-267 RB denotes before
T7755 258-260 IN denotes as
T7754 243-247 NN denotes LacZ
T7753 239-242 CC denotes and
T7752 234-238 NN denotes URA3
T7751 232-234 , denotes ,
T7750 228-232 NN denotes HIS3
T7749 248-257 NNS denotes reporters
T7748 224-227 DT denotes the
T7747 221-223 IN denotes of
T7746 210-220 NN denotes activation
T7745 206-209 IN denotes for
T7744 187-196 VBG denotes resulting
T7743 197-205 NNS denotes colonies
T7742 183-186 DT denotes the
T7741 175-182 VBG denotes testing
T7740 171-174 CC denotes and
T7739 169-170 -RRB- denotes )
T7738 164-169 NN denotes pPC86
T7737 161-163 IN denotes in
T7736 160-161 -LRB- denotes (
T7735 150-159 NN denotes construct
T7734 145-149 NN denotes prey
T7733 141-144 CD denotes one
T7732 137-140 CC denotes and
T7731 135-136 -RRB- denotes )
T7730 129-135 NN denotes pDBLeu
T7729 126-128 IN denotes in
T7728 125-126 -LRB- denotes (
T7727 115-124 NN denotes construct
T7726 110-114 NN denotes bait
T7725 106-109 CD denotes one
T7724 101-105 IN denotes with
T7723 89-94 NN denotes yeast
T7722 82-88 NN denotes MaV203
T7721 95-100 NNS denotes cells
T7720 72-81 JJ denotes competent
T7719 59-71 VBG denotes transforming
T7718 56-58 IN denotes by
T7717 44-48 VBD denotes were
T7716 49-55 VBN denotes tested
T7715 31-43 NNS denotes interactions
T7714 22-30 JJ denotes Specific
T7713 21-268 sentence denotes Specific interactions were tested by transforming competent MaV203 yeast cells with one bait construct (in pDBLeu) and one prey construct (in pPC86) and testing the resulting colonies for activation of the HIS3, URA3 and LacZ reporters as before.
T7712 9-21 NNS denotes interactions
T7711 0-8 JJ denotes Pairwise
R4882 T7711 T7712 amod Pairwise,interactions
R4883 T7714 T7715 amod Specific,interactions
R4884 T7715 T7716 nsubjpass interactions,tested
R4885 T7717 T7716 auxpass were,tested
R4886 T7718 T7716 prep by,tested
R4887 T7719 T7718 pcomp transforming,by
R4888 T7720 T7721 amod competent,cells
R4889 T7721 T7719 dobj cells,transforming
R4890 T7722 T7721 compound MaV203,cells
R4891 T7723 T7721 compound yeast,cells
R4892 T7724 T7721 prep with,cells
R4893 T7725 T7726 nummod one,bait
R4894 T7726 T7727 compound bait,construct
R4895 T7727 T7724 pobj construct,with
R4896 T7728 T7727 punct (,construct
R4897 T7729 T7727 prep in,construct
R4898 T7730 T7729 pobj pDBLeu,in
R4899 T7731 T7727 punct ),construct
R4900 T7732 T7727 cc and,construct
R4901 T7733 T7734 nummod one,prey
R4902 T7734 T7735 compound prey,construct
R4903 T7735 T7727 conj construct,construct
R4904 T7736 T7735 punct (,construct
R4905 T7737 T7735 prep in,construct
R4906 T7738 T7737 pobj pPC86,in
R4907 T7739 T7719 punct ),transforming
R4908 T7740 T7719 cc and,transforming
R4909 T7741 T7719 conj testing,transforming
R4910 T7742 T7743 det the,colonies
R4911 T7743 T7741 dobj colonies,testing
R4912 T7744 T7743 amod resulting,colonies
R4913 T7745 T7741 prep for,testing
R4914 T7746 T7745 pobj activation,for
R4915 T7747 T7746 prep of,activation
R4916 T7748 T7749 det the,reporters
R4917 T7749 T7747 pobj reporters,of
R4918 T7750 T7749 nmod HIS3,reporters
R4919 T7751 T7750 punct ", ",HIS3
R4920 T7752 T7750 conj URA3,HIS3
R4921 T7753 T7752 cc and,URA3
R4922 T7754 T7752 conj LacZ,URA3
R4923 T7755 T7741 prep as,testing
R4924 T7756 T7755 pcomp before,as
R4925 T7757 T7716 punct .,tested
R4926 T7759 T7760 aux To,create
R4927 T7760 T7761 advcl create,identified
R4928 T7762 T7763 det a,clone
R4929 T7763 T7760 dobj clone,create
R4930 T7764 T7765 amod full,length
R4931 T7765 T7763 compound length,clone
R4932 T7766 T7765 punct -,length
R4933 T7767 T7763 compound Homer3,clone
R4934 T7768 T7760 prep for,create
R4935 T7769 T7770 amod pairwise,tests
R4936 T7770 T7768 pobj tests,for
R4937 T7771 T7761 punct ", ",identified
R4938 T7772 T7773 det a,clone
R4939 T7773 T7761 nsubjpass clone,identified
R4940 T7774 T7773 compound cDNA,clone
R4941 T7775 T7776 punct (,clone
R4942 T7776 T7773 parataxis clone,clone
R4943 T7777 T7776 compound IMAGE,clone
R4944 T7778 T7776 nummod 3602414,clone
R4945 T7779 T7776 punct ", ",clone
R4946 T7780 T7781 compound accession,BE569374
R4947 T7781 T7776 appos BE569374,clone
R4948 T7782 T7781 compound number,BE569374
R4949 T7783 T7776 punct ),clone
R4950 T7784 T7773 acl containing,clone
R4951 T7785 T7786 det the,acids
R4952 T7786 T7784 dobj acids,containing
R4953 T7787 T7786 amod missing,acids
R4954 T7788 T7789 npadvmod N,terminal
R4955 T7789 T7786 amod terminal,acids
R4956 T7790 T7789 punct -,terminal
R4957 T7791 T7786 nummod 70,acids
R4958 T7792 T7786 compound amino,acids
R4959 T7793 T7761 auxpass was,identified
R4960 T7794 T7761 prep by,identified
R4961 T7795 T7796 det a,search
R4962 T7796 T7794 pobj search,by
R4963 T7797 T7796 compound BLAST,search
R4964 T7798 T7796 prep of,search
R4965 T7799 T7800 det the,database
R4966 T7800 T7798 pobj database,of
R4967 T7801 T7800 compound EST,database
R4968 T7802 T7803 compound nucleotide,sequence
R4969 T7803 T7800 compound sequence,database
R4970 T7804 T7761 cc and,identified
R4971 T7805 T7761 conj obtained,identified
R4972 T7806 T7805 prep from,obtained
R4973 T7807 T7808 det the,Centre
R4974 T7808 T7806 pobj Centre,from
R4975 T7809 T7810 compound Rosalind,Franklin
R4976 T7810 T7808 compound Franklin,Centre
R4977 T7811 T7808 prep for,Centre
R4978 T7812 T7813 compound Genomics,Research
R4979 T7813 T7811 pobj Research,for
R4980 T7814 T7761 punct .,identified
R4981 T7816 T7817 det The,fragment
R4982 T7817 T7819 nsubj fragment,amplified
R4983 T7818 T7817 amod missing,fragment
R4984 T7820 T7819 aux was,amplified
R4985 T7821 T7819 prep from,amplified
R4986 T7822 T7823 det the,clone
R4987 T7823 T7821 pobj clone,from
R4988 T7824 T7823 compound IMAGE,clone
R4989 T7825 T7819 prep by,amplified
R4990 T7826 T7825 pobj PCR,by
R4991 T7827 T7819 cc and,amplified
R4992 T7828 T7819 conj inserted,amplified
R4993 T7829 T7828 prep into,inserted
R4994 T7830 T7831 det the,plasmid
R4995 T7831 T7829 pobj plasmid,into
R4996 T7832 T7833 compound pPC86,Homer3
R4997 T7833 T7831 compound Homer3,plasmid
R4998 T7834 T7833 punct -,Homer3
R4999 T7835 T7836 aux to,create
R5000 T7836 T7819 advcl create,amplified
R5001 T7837 T7838 det a,construct
R5002 T7838 T7836 dobj construct,create
R5003 T7839 T7840 amod full,length
R5004 T7840 T7838 compound length,construct
R5005 T7841 T7840 punct -,length
R5006 T7842 T7838 compound expression,construct
R5007 T7843 T7819 punct .,amplified

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the bag model. You can change it to the chain model.

Id Subject Object Predicate Lexical cue
T7710 721-730 SO_EXT:engineered_biological_sequence denotes construct
T7709 710-720 GO:0010467 denotes expression
T7708 678-685 SO_EXT:0000155 denotes plasmid
T7707 671-677 PR_EXT:000008680 denotes Homer3
T7706 647-655 SO_EXT:sequence_insertion_process denotes inserted
T7705 630-635 SO_EXT:sequence_cloned_entity denotes clone
T7704 492-500 SO_EXT:biological_sequence denotes sequence
T7703 481-491 CHEBI_SO_EXT:nucleotide denotes nucleotide
T7702 477-480 SO_EXT:0000345 denotes EST
T7701 425-436 CHEBI_SO_EXT:amino_acid denotes amino acids
T7700 411-421 CHEBI_SO_EXT:N_terminus_or_N_terminal_region denotes N-terminal
T7699 346-351 SO_EXT:sequence_cloned_entity denotes clone
T7698 328-338 SO_EXT:0000317 denotes cDNA clone
T7697 300-305 SO_EXT:sequence_cloned_entity denotes clone
T7696 293-299 PR_EXT:000008680 denotes Homer3
T7695 243-247 PR_EXT:000033987 denotes LacZ
T7694 234-238 PR_EXT:P03962 denotes URA3
T7693 228-232 PR_EXT:P06633 denotes HIS3
T7692 150-159 SO_EXT:engineered_biological_sequence denotes construct
T7691 115-124 SO_EXT:engineered_biological_sequence denotes construct
T7690 95-100 CL_GO_EXT:cell denotes cells
T7689 89-94 NCBITaxon_EXT:yeast denotes yeast
T7688 59-71 GO:0009294 denotes transforming

craft-ca-core-dev

Below, discontinuous spans are shown in the bag model. You can change it to the chain model.

Id Subject Object Predicate Lexical cue
T7687 710-720 GO:0010467 denotes expression
T7686 678-685 SO:0000155 denotes plasmid
T7685 671-677 PR:000008680 denotes Homer3
T7684 477-480 SO:0000345 denotes EST
T7683 328-338 SO:0000317 denotes cDNA clone
T7682 293-299 PR:000008680 denotes Homer3
T7681 243-247 PR:000033987 denotes LacZ
T7680 234-238 PR:P03962 denotes URA3
T7679 228-232 PR:P06633 denotes HIS3
T7678 59-71 GO:0009294 denotes transforming