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MCAD IV2 undergoes gap repair upon homologous recombination at the endogenous Acadm locus resulting in a duplication of exons 8, 9, and 10 at the disrupted allele.\n(B) Southern blot analysis of EcoRI-digested genomic DNA from ES cells screened by PCR. Probe A, a DNA fragment consisting of a portion of exon 10 that is not present in the targeting vector, hybridizes to an endogenous 3.1-kb fragment and, upon homologous recombination, to a 13.2-kb fragment. Lane 1 represents a wild-type ES cell line, and Lane 2 and 3 represent targeted ES cell lines."}

{"project":"craft-ca-core-dev","denotations":[{"id":"T7397","span":{"begin":41,"end":46},"obj":"NCBITaxon:10088"},{"id":"T7398","span":{"begin":53,"end":57},"obj":"SO:0000704"},{"id":"T7399","span":{"begin":85,"end":101},"obj":"SO:0001644"},{"id":"T7400","span":{"begin":144,"end":148},"obj":"SO:0000147"},{"id":"T7401","span":{"begin":156,"end":164},"obj":"SO:0000357"},{"id":"T7402","span":{"begin":296,"end":301},"obj":"SO:0000147"},{"id":"T7403","span":{"begin":332,"end":338},"obj":"SO:0001023"},{"id":"T7404","span":{"begin":385,"end":392},"obj":"SO:0001026"},{"id":"T7405","span":{"begin":402,"end":410},"obj":"CL:0002322"},{"id":"T7406","span":{"begin":468,"end":483},"obj":"SO:0000852"},{"id":"T7407","span":{"begin":514,"end":530},"obj":"SO:0001644"},{"id":"T7408","span":{"begin":532,"end":542},"obj":"GO:0097617"},{"id":"T7409","span":{"begin":665,"end":672},"obj":"CL:0002322"},{"id":"T7410","span":{"begin":715,"end":722},"obj":"CL:0002322"}],"text":"Figure 1 Strategy for Disruption of the Mouse Acadm Gene\n(A) The MCAD IV2 insertion targeting vector with a deleted 1.3-kb region encompassing exon 10 and flanking sequences. MCAD IV2 undergoes gap repair upon homologous recombination at the endogenous Acadm locus resulting in a duplication of exons 8, 9, and 10 at the disrupted allele.\n(B) Southern blot analysis of EcoRI-digested genomic DNA from ES cells screened by PCR. Probe A, a DNA fragment consisting of a portion of exon 10 that is not present in the targeting vector, hybridizes to an endogenous 3.1-kb fragment and, upon homologous recombination, to a 13.2-kb fragment. Lane 1 represents a wild-type ES cell line, and Lane 2 and 3 represent targeted ES cell lines."}

{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T7411","span":{"begin":41,"end":46},"obj":"NCBITaxon:10088"},{"id":"T7412","span":{"begin":47,"end":52},"obj":"GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase"},{"id":"T7413","span":{"begin":53,"end":57},"obj":"SO_EXT:0000704"},{"id":"T7414","span":{"begin":66,"end":70},"obj":"GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase"},{"id":"T7415","span":{"begin":75,"end":84},"obj":"SO_EXT:sequence_insertion_entity_or_process"},{"id":"T7416","span":{"begin":85,"end":101},"obj":"SO_EXT:0001644"},{"id":"T7417","span":{"begin":109,"end":116},"obj":"SO_EXT:sequence_deletion_process"},{"id":"T7418","span":{"begin":122,"end":123},"obj":"CHEBI_SO_EXT:base"},{"id":"T7419","span":{"begin":144,"end":148},"obj":"SO_EXT:0000147"},{"id":"T7420","span":{"begin":156,"end":164},"obj":"SO:0000357"},{"id":"T7421","span":{"begin":165,"end":174},"obj":"SO_EXT:biological_sequence"},{"id":"T7422","span":{"begin":176,"end":180},"obj":"GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase"},{"id":"T7423","span":{"begin":195,"end":205},"obj":"GO_EXT:DNA_gap_repair"},{"id":"T7424","span":{"begin":222,"end":235},"obj":"GO_SO_EXT:sequence_rearrangement_process"},{"id":"T7425","span":{"begin":254,"end":259},"obj":"GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase"},{"id":"T7426","span":{"begin":281,"end":292},"obj":"SO_EXT:sequence_duplication_entity_or_process"},{"id":"T7427","span":{"begin":296,"end":301},"obj":"SO_EXT:0000147"},{"id":"T7428","span":{"begin":332,"end":338},"obj":"SO_EXT:0001023"},{"id":"T7429","span":{"begin":385,"end":396},"obj":"SO_EXT:genomic_DNA"},{"id":"T7430","span":{"begin":393,"end":396},"obj":"CHEBI_SO_EXT:DNA"},{"id":"T7431","span":{"begin":402,"end":410},"obj":"CL:0002322"},{"id":"T7432","span":{"begin":405,"end":410},"obj":"CL_GO_EXT:cell"},{"id":"T7433","span":{"begin":428,"end":433},"obj":"CHEBI_SO_EXT:molecular_probe"},{"id":"T7434","span":{"begin":439,"end":442},"obj":"CHEBI_SO_EXT:DNA"},{"id":"T7435","span":{"begin":468,"end":483},"obj":"SO_EXT:0000852"},{"id":"T7436","span":{"begin":514,"end":530},"obj":"SO_EXT:0001644"},{"id":"T7437","span":{"begin":532,"end":542},"obj":"GO:0097617"},{"id":"T7438","span":{"begin":565,"end":566},"obj":"CHEBI_SO_EXT:base"},{"id":"T7439","span":{"begin":597,"end":610},"obj":"GO_SO_EXT:sequence_rearrangement_process"},{"id":"T7440","span":{"begin":623,"end":624},"obj":"CHEBI_SO_EXT:base"},{"id":"T7441","span":{"begin":655,"end":664},"obj":"SO_EXT:wild_type_entity_or_quality"},{"id":"T7442","span":{"begin":665,"end":672},"obj":"CL:0002322"},{"id":"T7443","span":{"begin":668,"end":672},"obj":"CL_GO_EXT:cell"},{"id":"T7444","span":{"begin":715,"end":722},"obj":"CL:0002322"},{"id":"T7445","span":{"begin":718,"end":722},"obj":"CL_GO_EXT:cell"}],"text":"Figure 1 Strategy for Disruption of the Mouse Acadm Gene\n(A) The MCAD IV2 insertion targeting vector with a deleted 1.3-kb region encompassing exon 10 and flanking sequences. MCAD IV2 undergoes gap repair upon homologous recombination at the endogenous Acadm locus resulting in a duplication of exons 8, 9, and 10 at the disrupted allele.\n(B) Southern blot analysis of EcoRI-digested genomic DNA from ES cells screened by PCR. Probe A, a DNA fragment consisting of a portion of exon 10 that is not present in the targeting vector, hybridizes to an endogenous 3.1-kb fragment and, upon homologous recombination, to a 13.2-kb fragment. Lane 1 represents a wild-type ES cell line, and Lane 2 and 3 represent targeted ES cell lines."}