| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T11284 |
17599-17600 |
-LRB- |
denotes |
( |
| T11285 |
17617-17621 |
JJ |
denotes |
left |
| T11286 |
17600-17606 |
NN |
denotes |
Figure |
| T11287 |
17607-17609 |
CD |
denotes |
5C |
| T11288 |
17609-17611 |
, |
denotes |
, |
| T11289 |
17611-17616 |
JJ |
denotes |
lower |
| T11290 |
17621-17622 |
-RRB- |
denotes |
) |
| T11291 |
17622-17623 |
. |
denotes |
. |
| T11292 |
17623-17779 |
sentence |
denotes |
When expressed in wild-type AQP2 cells, however, AQP2-F204V-GFP localized to the apical surface to varying degrees (Figure 5C, lower right images [i–iii]). |
| T8951 |
8-10 |
IN |
denotes |
In |
| T8952 |
191-196 |
VBD |
denotes |
found |
| T8953 |
11-12 |
DT |
denotes |
a |
| T8954 |
29-35 |
NN |
denotes |
screen |
| T8955 |
13-20 |
JJ |
denotes |
forward |
| T8956 |
21-28 |
JJ |
denotes |
genetic |
| T8957 |
36-40 |
WDT |
denotes |
that |
| T8958 |
41-45 |
VBD |
denotes |
used |
| T8959 |
46-62 |
NN |
denotes |
ethylnitrosourea |
| T8960 |
63-64 |
-LRB- |
denotes |
( |
| T8961 |
64-67 |
NN |
denotes |
ENU |
| T8962 |
67-68 |
-RRB- |
denotes |
) |
| T8963 |
69-71 |
TO |
denotes |
to |
| T8964 |
72-78 |
VB |
denotes |
induce |
| T8965 |
79-88 |
NNS |
denotes |
mutations |
| T8966 |
89-91 |
IN |
denotes |
in |
| T8967 |
92-93 |
DT |
denotes |
a |
| T8968 |
102-108 |
NN |
denotes |
animal |
| T8969 |
94-101 |
NN |
denotes |
founder |
| T8970 |
109-114 |
WP$ |
denotes |
whose |
| T8971 |
115-124 |
NN |
denotes |
offspring |
| T8972 |
135-143 |
VBN |
denotes |
screened |
| T8973 |
125-129 |
VBD |
denotes |
were |
| T8974 |
130-134 |
RB |
denotes |
then |
| T8975 |
144-147 |
IN |
denotes |
for |
| T8976 |
148-156 |
JJ |
denotes |
abnormal |
| T8977 |
168-178 |
NN |
denotes |
metabolism |
| T8978 |
157-162 |
JJ |
denotes |
whole |
| T8979 |
163-167 |
NN |
denotes |
body |
| T8980 |
179-180 |
-LRB- |
denotes |
[ |
| T8981 |
183-185 |
CD |
denotes |
20 |
| T8982 |
180-182 |
CD |
denotes |
19 |
| T8983 |
182-183 |
, |
denotes |
, |
| T8984 |
185-186 |
-RRB- |
denotes |
] |
| T8985 |
186-188 |
, |
denotes |
, |
| T8986 |
188-190 |
PRP |
denotes |
we |
| T8987 |
197-198 |
DT |
denotes |
a |
| T8988 |
199-205 |
NN |
denotes |
family |
| T8989 |
206-208 |
IN |
denotes |
of |
| T8990 |
209-213 |
NNS |
denotes |
mice |
| T8991 |
214-218 |
WDT |
denotes |
that |
| T8992 |
219-227 |
VBD |
denotes |
urinated |
| T8993 |
228-231 |
CC |
denotes |
and |
| T8994 |
232-237 |
VBD |
denotes |
drank |
| T8995 |
238-249 |
RB |
denotes |
excessively |
| T8996 |
249-250 |
. |
denotes |
. |
| T8997 |
250-379 |
sentence |
denotes |
Serum and urine analysis showed that plasma glucose levels were normal and there was no glucose in the urine (unpublished data). |
| T8998 |
251-256 |
NN |
denotes |
Serum |
| T8999 |
267-275 |
NN |
denotes |
analysis |
| T9000 |
257-260 |
CC |
denotes |
and |
| T9001 |
261-266 |
NN |
denotes |
urine |
| T9002 |
276-282 |
VBD |
denotes |
showed |
| T9003 |
283-287 |
IN |
denotes |
that |
| T9004 |
310-314 |
VBD |
denotes |
were |
| T9005 |
288-294 |
NN |
denotes |
plasma |
| T9006 |
303-309 |
NNS |
denotes |
levels |
| T9007 |
295-302 |
NN |
denotes |
glucose |
| T9008 |
315-321 |
JJ |
denotes |
normal |
| T9009 |
322-325 |
CC |
denotes |
and |
| T9010 |
326-331 |
EX |
denotes |
there |
| T9011 |
332-335 |
VBD |
denotes |
was |
| T9012 |
336-338 |
DT |
denotes |
no |
| T9013 |
339-346 |
NN |
denotes |
glucose |
| T9014 |
347-349 |
IN |
denotes |
in |
| T9015 |
350-353 |
DT |
denotes |
the |
| T9016 |
354-359 |
NN |
denotes |
urine |
| T9017 |
360-361 |
-LRB- |
denotes |
( |
| T9018 |
373-377 |
NNS |
denotes |
data |
| T9019 |
361-372 |
JJ |
denotes |
unpublished |
| T9020 |
377-378 |
-RRB- |
denotes |
) |
| T9021 |
378-379 |
. |
denotes |
. |
| T9022 |
379-429 |
sentence |
denotes |
Hence, this was an example of diabetes insipidus. |
| T9023 |
380-385 |
RB |
denotes |
Hence |
| T9024 |
392-395 |
VBD |
denotes |
was |
| T9025 |
385-387 |
, |
denotes |
, |
| T9026 |
387-391 |
DT |
denotes |
this |
| T9027 |
396-398 |
DT |
denotes |
an |
| T9028 |
399-406 |
NN |
denotes |
example |
| T9029 |
407-409 |
IN |
denotes |
of |
| T9030 |
410-418 |
NN |
denotes |
diabetes |
| T9031 |
419-428 |
NN |
denotes |
insipidus |
| T9032 |
428-429 |
. |
denotes |
. |
| T9033 |
429-541 |
sentence |
denotes |
The disorder in these mice segregated in a monogenic, autosomal recessive manner, making Aqp2 a candidate gene. |
| T9034 |
430-433 |
DT |
denotes |
The |
| T9035 |
434-442 |
NN |
denotes |
disorder |
| T9036 |
457-467 |
VBD |
denotes |
segregated |
| T9037 |
443-445 |
IN |
denotes |
in |
| T9038 |
446-451 |
DT |
denotes |
these |
| T9039 |
452-456 |
NNS |
denotes |
mice |
| T9040 |
468-470 |
IN |
denotes |
in |
| T9041 |
471-472 |
DT |
denotes |
a |
| T9042 |
504-510 |
NN |
denotes |
manner |
| T9043 |
473-482 |
JJ |
denotes |
monogenic |
| T9044 |
482-484 |
, |
denotes |
, |
| T9045 |
484-493 |
JJ |
denotes |
autosomal |
| T9046 |
494-503 |
JJ |
denotes |
recessive |
| T9047 |
510-512 |
, |
denotes |
, |
| T9048 |
512-518 |
VBG |
denotes |
making |
| T9049 |
519-523 |
NN |
denotes |
Aqp2 |
| T9050 |
536-540 |
NN |
denotes |
gene |
| T9051 |
524-525 |
DT |
denotes |
a |
| T9052 |
526-535 |
NN |
denotes |
candidate |
| T9053 |
540-541 |
. |
denotes |
. |
| T9054 |
541-770 |
sentence |
denotes |
Sequencing of Aqp2 coding region of affected mice identified a thymine to guanine (T to G) transversion (Figure 1A), which is predicted to lead to a valine for phenylalanine substitution at amino acid 204 of the protein (F204V). |
| T9055 |
542-552 |
NN |
denotes |
Sequencing |
| T9056 |
592-602 |
VBD |
denotes |
identified |
| T9057 |
553-555 |
IN |
denotes |
of |
| T9058 |
556-560 |
NN |
denotes |
Aqp2 |
| T9059 |
568-574 |
NN |
denotes |
region |
| T9060 |
561-567 |
NN |
denotes |
coding |
| T9061 |
575-577 |
IN |
denotes |
of |
| T9062 |
578-586 |
VBN |
denotes |
affected |
| T9063 |
587-591 |
NNS |
denotes |
mice |
| T9064 |
603-604 |
DT |
denotes |
a |
| T9065 |
633-645 |
NN |
denotes |
transversion |
| T9066 |
605-612 |
NN |
denotes |
thymine |
| T9067 |
613-615 |
IN |
denotes |
to |
| T9068 |
616-623 |
NN |
denotes |
guanine |
| T9069 |
624-625 |
-LRB- |
denotes |
( |
| T9070 |
625-626 |
NN |
denotes |
T |
| T9071 |
627-629 |
IN |
denotes |
to |
| T9072 |
630-631 |
NN |
denotes |
G |
| T9073 |
631-632 |
-RRB- |
denotes |
) |
| T9074 |
646-647 |
-LRB- |
denotes |
( |
| T9075 |
647-653 |
NN |
denotes |
Figure |
| T9076 |
654-656 |
CD |
denotes |
1A |
| T9077 |
656-657 |
-RRB- |
denotes |
) |
| T9078 |
657-659 |
, |
denotes |
, |
| T9079 |
659-664 |
WDT |
denotes |
which |
| T9080 |
668-677 |
VBN |
denotes |
predicted |
| T9081 |
665-667 |
VBZ |
denotes |
is |
| T9082 |
678-680 |
TO |
denotes |
to |
| T9083 |
681-685 |
VB |
denotes |
lead |
| T9084 |
686-688 |
IN |
denotes |
to |
| T9085 |
689-690 |
DT |
denotes |
a |
| T9086 |
716-728 |
NN |
denotes |
substitution |
| T9087 |
691-697 |
NN |
denotes |
valine |
| T9088 |
698-701 |
IN |
denotes |
for |
| T9089 |
702-715 |
NN |
denotes |
phenylalanine |
| T9090 |
729-731 |
IN |
denotes |
at |
| T9091 |
732-737 |
NN |
denotes |
amino |
| T9092 |
738-742 |
NN |
denotes |
acid |
| T9093 |
743-746 |
CD |
denotes |
204 |
| T9094 |
747-749 |
IN |
denotes |
of |
| T9095 |
750-753 |
DT |
denotes |
the |
| T9096 |
754-761 |
NN |
denotes |
protein |
| T9097 |
762-763 |
-LRB- |
denotes |
( |
| T9098 |
763-768 |
NN |
denotes |
F204V |
| T9099 |
768-769 |
-RRB- |
denotes |
) |
| T9100 |
769-770 |
. |
denotes |
. |
| T9101 |
770-1960 |
sentence |
denotes |
Figure 1 Analysis of Aqp2 Sequence and Phenotype in Mutant Mice
(A) Chromatographic traces of Aqp2 F204V mutation. The box shows the mutated codon, TTC (Phe) to GTC (Val) at position 204.
WT, wild type; Mut, mutant.
(B) Amino acid conservation of mouse AQP2 (residues 194–214). The boxed residue indicates phenylalanine at position 204.
hAQP2, human AQP2; mAQP1, mouse AQP1; mAQP2, mouse AQP2; rAQP2, rat AQP2; xAQP2, Xenopus AQP2.
(C) Urine production (ml) and water consumption (ml) of 58 F2 mice over a 24-h period (both sexes, aged 10–22 wk). Mutant mice (black squares) exhibit overt polyuria and polydipsia compared to littermate wild-type (white triangles) and heterozygous (grey circles) mice.
(D) Urine osmolality and concentrating ability in Aqp2 mutant and their littermates (10–22 wk, both sexes), before (white bars) and after (black bars) dDAVP treatment. Wild type (WT; n = 12); heterozygote (Het; n = 20); mutant (Mut; n = 9). Data represent averages ± standard error of the mean, **p < 0.01; ***p < 0.001. AQP2 is a six-transmembrane water channel, and F204 lies near the extracellular face of the sixth membrane spanning domain, a region rich in hydrophobic amino acids. |
| T9102 |
1795-1799 |
NN |
denotes |
AQP2 |
| T9103 |
1800-1802 |
VBZ |
denotes |
is |
| T9104 |
1803-1804 |
DT |
denotes |
a |
| T9105 |
1829-1836 |
NN |
denotes |
channel |
| T9106 |
1805-1808 |
CD |
denotes |
six |
| T9107 |
1809-1822 |
NN |
denotes |
transmembrane |
| T9108 |
1808-1809 |
HYPH |
denotes |
- |
| T9109 |
1823-1828 |
NN |
denotes |
water |
| T9110 |
1836-1838 |
, |
denotes |
, |
| T9111 |
1838-1841 |
CC |
denotes |
and |
| T9112 |
1842-1846 |
NN |
denotes |
F204 |
| T9113 |
1847-1851 |
VBZ |
denotes |
lies |
| T9114 |
1852-1856 |
IN |
denotes |
near |
| T9115 |
1857-1860 |
DT |
denotes |
the |
| T9116 |
1875-1879 |
NN |
denotes |
face |
| T9117 |
1861-1874 |
JJ |
denotes |
extracellular |
| T9118 |
1880-1882 |
IN |
denotes |
of |
| T9119 |
1883-1886 |
DT |
denotes |
the |
| T9120 |
1911-1917 |
NN |
denotes |
domain |
| T9121 |
1887-1892 |
JJ |
denotes |
sixth |
| T9122 |
1893-1901 |
NN |
denotes |
membrane |
| T9123 |
1902-1910 |
VBG |
denotes |
spanning |
| T9124 |
1917-1919 |
, |
denotes |
, |
| T9125 |
1919-1920 |
DT |
denotes |
a |
| T9126 |
1921-1927 |
NN |
denotes |
region |
| T9127 |
1928-1932 |
JJ |
denotes |
rich |
| T9128 |
1933-1935 |
IN |
denotes |
in |
| T9129 |
1936-1947 |
JJ |
denotes |
hydrophobic |
| T9130 |
1954-1959 |
NNS |
denotes |
acids |
| T9131 |
1948-1953 |
NN |
denotes |
amino |
| T9132 |
1959-1960 |
. |
denotes |
. |
| T9133 |
1960-2045 |
sentence |
denotes |
This and the other membrane-spanning domains are conserved among vertebrate species. |
| T9134 |
1961-1965 |
DT |
denotes |
This |
| T9135 |
2010-2019 |
VBN |
denotes |
conserved |
| T9136 |
1966-1969 |
CC |
denotes |
and |
| T9137 |
1970-1973 |
DT |
denotes |
the |
| T9138 |
1974-1979 |
JJ |
denotes |
other |
| T9139 |
1998-2005 |
NNS |
denotes |
domains |
| T9140 |
1980-1988 |
NN |
denotes |
membrane |
| T9141 |
1989-1997 |
VBG |
denotes |
spanning |
| T9142 |
1988-1989 |
HYPH |
denotes |
- |
| T9143 |
2006-2009 |
VBP |
denotes |
are |
| T9144 |
2020-2025 |
IN |
denotes |
among |
| T9145 |
2026-2036 |
JJ |
denotes |
vertebrate |
| T9146 |
2037-2044 |
NNS |
denotes |
species |
| T9147 |
2044-2045 |
. |
denotes |
. |
| T9148 |
2045-2210 |
sentence |
denotes |
The phenylalanine at position 204 is particularly well conserved (Figure 1B), not only among vertebrate AQP2 proteins, but also among others members of this family. |
| T9149 |
2046-2049 |
DT |
denotes |
The |
| T9150 |
2050-2063 |
NN |
denotes |
phenylalanine |
| T9151 |
2080-2082 |
VBZ |
denotes |
is |
| T9152 |
2064-2066 |
IN |
denotes |
at |
| T9153 |
2067-2075 |
NN |
denotes |
position |
| T9154 |
2076-2079 |
CD |
denotes |
204 |
| T9155 |
2083-2095 |
RB |
denotes |
particularly |
| T9156 |
2101-2110 |
VBN |
denotes |
conserved |
| T9157 |
2096-2100 |
RB |
denotes |
well |
| T9158 |
2111-2112 |
-LRB- |
denotes |
( |
| T9159 |
2112-2118 |
NN |
denotes |
Figure |
| T9160 |
2119-2121 |
CD |
denotes |
1B |
| T9161 |
2121-2122 |
-RRB- |
denotes |
) |
| T9162 |
2122-2124 |
, |
denotes |
, |
| T9163 |
2124-2127 |
RB |
denotes |
not |
| T9164 |
2133-2138 |
IN |
denotes |
among |
| T9165 |
2128-2132 |
RB |
denotes |
only |
| T9166 |
2139-2149 |
JJ |
denotes |
vertebrate |
| T9167 |
2155-2163 |
NN |
denotes |
proteins |
| T9168 |
2150-2154 |
NN |
denotes |
AQP2 |
| T9169 |
2163-2165 |
, |
denotes |
, |
| T9170 |
2165-2168 |
CC |
denotes |
but |
| T9171 |
2169-2173 |
RB |
denotes |
also |
| T9172 |
2174-2179 |
IN |
denotes |
among |
| T9173 |
2180-2186 |
NNS |
denotes |
others |
| T9174 |
2187-2194 |
NNS |
denotes |
members |
| T9175 |
2195-2197 |
IN |
denotes |
of |
| T9176 |
2198-2202 |
DT |
denotes |
this |
| T9177 |
2203-2209 |
NN |
denotes |
family |
| T9178 |
2209-2210 |
. |
denotes |
. |
| T9179 |
2210-2417 |
sentence |
denotes |
Aqp2F204V/F204V mice have dramatically increased urine production, in some cases producing an amount of urine in 24 h that exceeds their body weight, compared to their heterozygous or wild-type littermates. |
| T9180 |
2211-2220 |
NN |
denotes |
Aqp2F204V |
| T9181 |
2221-2226 |
NN |
denotes |
F204V |
| T9182 |
2220-2221 |
HYPH |
denotes |
/ |
| T9183 |
2227-2231 |
NNS |
denotes |
mice |
| T9184 |
2232-2236 |
VBP |
denotes |
have |
| T9185 |
2237-2249 |
RB |
denotes |
dramatically |
| T9186 |
2250-2259 |
VBN |
denotes |
increased |
| T9187 |
2266-2276 |
NN |
denotes |
production |
| T9188 |
2260-2265 |
NN |
denotes |
urine |
| T9189 |
2276-2278 |
, |
denotes |
, |
| T9190 |
2278-2280 |
IN |
denotes |
in |
| T9191 |
2292-2301 |
VBG |
denotes |
producing |
| T9192 |
2281-2285 |
DT |
denotes |
some |
| T9193 |
2286-2291 |
NNS |
denotes |
cases |
| T9194 |
2302-2304 |
DT |
denotes |
an |
| T9195 |
2305-2311 |
NN |
denotes |
amount |
| T9196 |
2312-2314 |
IN |
denotes |
of |
| T9197 |
2315-2320 |
NN |
denotes |
urine |
| T9198 |
2321-2323 |
IN |
denotes |
in |
| T9199 |
2324-2326 |
CD |
denotes |
24 |
| T9200 |
2327-2328 |
NN |
denotes |
h |
| T9201 |
2329-2333 |
WDT |
denotes |
that |
| T9202 |
2334-2341 |
VBZ |
denotes |
exceeds |
| T9203 |
2342-2347 |
PRP$ |
denotes |
their |
| T9204 |
2353-2359 |
NN |
denotes |
weight |
| T9205 |
2348-2352 |
NN |
denotes |
body |
| T9206 |
2359-2361 |
, |
denotes |
, |
| T9207 |
2361-2369 |
VBN |
denotes |
compared |
| T9208 |
2370-2372 |
IN |
denotes |
to |
| T9209 |
2373-2378 |
PRP$ |
denotes |
their |
| T9210 |
2405-2416 |
NNS |
denotes |
littermates |
| T9211 |
2379-2391 |
JJ |
denotes |
heterozygous |
| T9212 |
2392-2394 |
CC |
denotes |
or |
| T9213 |
2395-2399 |
JJ |
denotes |
wild |
| T9214 |
2400-2404 |
NN |
denotes |
type |
| T9215 |
2399-2400 |
HYPH |
denotes |
- |
| T9216 |
2416-2417 |
. |
denotes |
. |
| T9217 |
2417-2521 |
sentence |
denotes |
Such loss of water would rapidly lead to dehydration were it not compensated by increased water intake. |
| T9218 |
2418-2422 |
JJ |
denotes |
Such |
| T9219 |
2423-2427 |
NN |
denotes |
loss |
| T9220 |
2451-2455 |
VB |
denotes |
lead |
| T9221 |
2428-2430 |
IN |
denotes |
of |
| T9222 |
2431-2436 |
NN |
denotes |
water |
| T9223 |
2437-2442 |
MD |
denotes |
would |
| T9224 |
2443-2450 |
RB |
denotes |
rapidly |
| T9225 |
2456-2458 |
IN |
denotes |
to |
| T9226 |
2459-2470 |
NN |
denotes |
dehydration |
| T9227 |
2471-2475 |
VBD |
denotes |
were |
| T9228 |
2483-2494 |
VBN |
denotes |
compensated |
| T9229 |
2476-2478 |
PRP |
denotes |
it |
| T9230 |
2479-2482 |
RB |
denotes |
not |
| T9231 |
2495-2497 |
IN |
denotes |
by |
| T9232 |
2498-2507 |
VBN |
denotes |
increased |
| T9233 |
2514-2520 |
NN |
denotes |
intake |
| T9234 |
2508-2513 |
NN |
denotes |
water |
| T9235 |
2520-2521 |
. |
denotes |
. |
| T9236 |
2521-2656 |
sentence |
denotes |
Indeed, mutant mice also dramatically increase their water intake (Figure 1C) compared to their heterozygous or wild-type littermates. |
| T9237 |
2522-2528 |
RB |
denotes |
Indeed |
| T9238 |
2560-2568 |
VB |
denotes |
increase |
| T9239 |
2528-2530 |
, |
denotes |
, |
| T9240 |
2530-2536 |
NN |
denotes |
mutant |
| T9241 |
2537-2541 |
NNS |
denotes |
mice |
| T9242 |
2542-2546 |
RB |
denotes |
also |
| T9243 |
2547-2559 |
RB |
denotes |
dramatically |
| T9244 |
2569-2574 |
PRP$ |
denotes |
their |
| T9245 |
2581-2587 |
NN |
denotes |
intake |
| T9246 |
2575-2580 |
NN |
denotes |
water |
| T9247 |
2588-2589 |
-LRB- |
denotes |
( |
| T9248 |
2589-2595 |
NN |
denotes |
Figure |
| T9249 |
2596-2598 |
CD |
denotes |
1C |
| T9250 |
2598-2599 |
-RRB- |
denotes |
) |
| T9251 |
2600-2608 |
VBN |
denotes |
compared |
| T9252 |
2609-2611 |
IN |
denotes |
to |
| T9253 |
2612-2617 |
PRP$ |
denotes |
their |
| T9254 |
2644-2655 |
NNS |
denotes |
littermates |
| T9255 |
2618-2630 |
JJ |
denotes |
heterozygous |
| T9256 |
2631-2633 |
CC |
denotes |
or |
| T9257 |
2634-2638 |
JJ |
denotes |
wild |
| T9258 |
2639-2643 |
NN |
denotes |
type |
| T9259 |
2638-2639 |
HYPH |
denotes |
- |
| T9260 |
2655-2656 |
. |
denotes |
. |
| T9261 |
2656-2807 |
sentence |
denotes |
This phenotype—increased urinary output and water intake—showed complete concordance with homozygosity of the F204V mutation in the 58 animals tested. |
| T9262 |
2657-2661 |
DT |
denotes |
This |
| T9263 |
2662-2671 |
NN |
denotes |
phenotype |
| T9264 |
2714-2720 |
VBD |
denotes |
showed |
| T9265 |
2671-2672 |
, |
denotes |
— |
| T9266 |
2672-2681 |
VBN |
denotes |
increased |
| T9267 |
2690-2696 |
NN |
denotes |
output |
| T9268 |
2682-2689 |
JJ |
denotes |
urinary |
| T9269 |
2697-2700 |
CC |
denotes |
and |
| T9270 |
2701-2706 |
NN |
denotes |
water |
| T9271 |
2707-2713 |
NN |
denotes |
intake |
| T9272 |
2713-2714 |
, |
denotes |
— |
| T9273 |
2721-2729 |
JJ |
denotes |
complete |
| T9274 |
2730-2741 |
NN |
denotes |
concordance |
| T9275 |
2742-2746 |
IN |
denotes |
with |
| T9276 |
2747-2759 |
NN |
denotes |
homozygosity |
| T9277 |
2760-2762 |
IN |
denotes |
of |
| T9278 |
2763-2766 |
DT |
denotes |
the |
| T9279 |
2773-2781 |
NN |
denotes |
mutation |
| T9280 |
2767-2772 |
NN |
denotes |
F204V |
| T9281 |
2782-2784 |
IN |
denotes |
in |
| T9282 |
2785-2788 |
DT |
denotes |
the |
| T9283 |
2792-2799 |
NNS |
denotes |
animals |
| T9284 |
2789-2791 |
CD |
denotes |
58 |
| T9285 |
2800-2806 |
VBN |
denotes |
tested |
| T9286 |
2806-2807 |
. |
denotes |
. |
| T9287 |
2807-2897 |
sentence |
denotes |
Diabetes insipidus can be defined as an inability to concentrate urine where appropriate. |
| T9288 |
2808-2816 |
NN |
denotes |
Diabetes |
| T9289 |
2817-2826 |
NN |
denotes |
insipidus |
| T9290 |
2834-2841 |
VBN |
denotes |
defined |
| T9291 |
2827-2830 |
MD |
denotes |
can |
| T9292 |
2831-2833 |
VB |
denotes |
be |
| T9293 |
2842-2844 |
IN |
denotes |
as |
| T9294 |
2845-2847 |
DT |
denotes |
an |
| T9295 |
2848-2857 |
NN |
denotes |
inability |
| T9296 |
2858-2860 |
TO |
denotes |
to |
| T9297 |
2861-2872 |
VB |
denotes |
concentrate |
| T9298 |
2873-2878 |
NN |
denotes |
urine |
| T9299 |
2879-2884 |
WRB |
denotes |
where |
| T9300 |
2885-2896 |
JJ |
denotes |
appropriate |
| T9301 |
2896-2897 |
. |
denotes |
. |
| T9302 |
2897-3008 |
sentence |
denotes |
Compared to wild-type or heterozygous littermates, Aqp2F204V/F204V mice produce very dilute urine (Figure 1D). |
| T9303 |
2898-2906 |
VBN |
denotes |
Compared |
| T9304 |
2970-2977 |
VBP |
denotes |
produce |
| T9305 |
2907-2909 |
IN |
denotes |
to |
| T9306 |
2910-2914 |
JJ |
denotes |
wild |
| T9307 |
2915-2919 |
NN |
denotes |
type |
| T9308 |
2914-2915 |
HYPH |
denotes |
- |
| T9309 |
2936-2947 |
NNS |
denotes |
littermates |
| T9310 |
2920-2922 |
CC |
denotes |
or |
| T9311 |
2923-2935 |
JJ |
denotes |
heterozygous |
| T9312 |
2947-2949 |
, |
denotes |
, |
| T9313 |
2949-2958 |
NN |
denotes |
Aqp2F204V |
| T9314 |
2959-2964 |
NN |
denotes |
F204V |
| T9315 |
2958-2959 |
HYPH |
denotes |
/ |
| T9316 |
2965-2969 |
NNS |
denotes |
mice |
| T9317 |
2978-2982 |
RB |
denotes |
very |
| T9318 |
2983-2989 |
JJ |
denotes |
dilute |
| T9319 |
2990-2995 |
NN |
denotes |
urine |
| T9320 |
2996-2997 |
-LRB- |
denotes |
( |
| T9321 |
2997-3003 |
NN |
denotes |
Figure |
| T9322 |
3004-3006 |
CD |
denotes |
1D |
| T9323 |
3006-3007 |
-RRB- |
denotes |
) |
| T9324 |
3007-3008 |
. |
denotes |
. |
| T9325 |
3008-3128 |
sentence |
denotes |
Basal urine concentration in mutant mice is about 161 mOsm, compared to about 1,293 mOsm in wild-type mice (p < 0.001). |
| T9326 |
3009-3014 |
JJ |
denotes |
Basal |
| T9327 |
3021-3034 |
NN |
denotes |
concentration |
| T9328 |
3015-3020 |
NN |
denotes |
urine |
| T9329 |
3050-3052 |
VBZ |
denotes |
is |
| T9330 |
3035-3037 |
IN |
denotes |
in |
| T9331 |
3038-3044 |
NN |
denotes |
mutant |
| T9332 |
3045-3049 |
NNS |
denotes |
mice |
| T9333 |
3053-3058 |
RB |
denotes |
about |
| T9334 |
3059-3062 |
CD |
denotes |
161 |
| T9335 |
3063-3067 |
NN |
denotes |
mOsm |
| T9336 |
3067-3069 |
, |
denotes |
, |
| T9337 |
3069-3077 |
VBN |
denotes |
compared |
| T9338 |
3078-3080 |
IN |
denotes |
to |
| T9339 |
3081-3086 |
IN |
denotes |
about |
| T9340 |
3087-3092 |
CD |
denotes |
1,293 |
| T9341 |
3093-3097 |
NN |
denotes |
mOsm |
| T9342 |
3098-3100 |
IN |
denotes |
in |
| T9343 |
3101-3105 |
JJ |
denotes |
wild |
| T9344 |
3106-3110 |
NN |
denotes |
type |
| T9345 |
3105-3106 |
HYPH |
denotes |
- |
| T9346 |
3111-3115 |
NNS |
denotes |
mice |
| T9347 |
3116-3117 |
-LRB- |
denotes |
( |
| T9348 |
3121-3126 |
CD |
denotes |
0.001 |
| T9349 |
3117-3118 |
NN |
denotes |
p |
| T9350 |
3119-3120 |
SYM |
denotes |
< |
| T9351 |
3126-3127 |
-RRB- |
denotes |
) |
| T9352 |
3127-3128 |
. |
denotes |
. |
| T9353 |
3128-3271 |
sentence |
denotes |
Normally, urine concentration is under the control of the hypothalamus, which, in response to hypovolemia or hypernatremia [21], secretes AVP. |
| T9354 |
3129-3137 |
RB |
denotes |
Normally |
| T9355 |
3159-3161 |
VBZ |
denotes |
is |
| T9356 |
3137-3139 |
, |
denotes |
, |
| T9357 |
3139-3144 |
NN |
denotes |
urine |
| T9358 |
3145-3158 |
NN |
denotes |
concentration |
| T9359 |
3162-3167 |
IN |
denotes |
under |
| T9360 |
3168-3171 |
DT |
denotes |
the |
| T9361 |
3172-3179 |
NN |
denotes |
control |
| T9362 |
3180-3182 |
IN |
denotes |
of |
| T9363 |
3183-3186 |
DT |
denotes |
the |
| T9364 |
3187-3199 |
NN |
denotes |
hypothalamus |
| T9365 |
3199-3201 |
, |
denotes |
, |
| T9366 |
3201-3206 |
WDT |
denotes |
which |
| T9367 |
3258-3266 |
VBZ |
denotes |
secretes |
| T9368 |
3206-3208 |
, |
denotes |
, |
| T9369 |
3208-3210 |
IN |
denotes |
in |
| T9370 |
3211-3219 |
NN |
denotes |
response |
| T9371 |
3220-3222 |
IN |
denotes |
to |
| T9372 |
3223-3234 |
NN |
denotes |
hypovolemia |
| T9373 |
3235-3237 |
CC |
denotes |
or |
| T9374 |
3238-3251 |
NN |
denotes |
hypernatremia |
| T9375 |
3252-3253 |
-LRB- |
denotes |
[ |
| T9376 |
3253-3255 |
CD |
denotes |
21 |
| T9377 |
3255-3256 |
-RRB- |
denotes |
] |
| T9378 |
3256-3258 |
, |
denotes |
, |
| T9379 |
3267-3270 |
NN |
denotes |
AVP |
| T9380 |
3270-3271 |
. |
denotes |
. |
| T9381 |
3271-3397 |
sentence |
denotes |
The synthetic AVP analog, 1-deamino-8-D-arginine vasopressin (dDAVP; also called desmopressin), is a potent agonist of AVPR2. |
| T9382 |
3272-3275 |
DT |
denotes |
The |
| T9383 |
3290-3296 |
NN |
denotes |
analog |
| T9384 |
3276-3285 |
JJ |
denotes |
synthetic |
| T9385 |
3286-3289 |
NN |
denotes |
AVP |
| T9386 |
3368-3370 |
VBZ |
denotes |
is |
| T9387 |
3296-3298 |
, |
denotes |
, |
| T9388 |
3298-3299 |
CD |
denotes |
1 |
| T9389 |
3300-3307 |
NN |
denotes |
deamino |
| T9390 |
3299-3300 |
HYPH |
denotes |
- |
| T9391 |
3312-3320 |
NN |
denotes |
arginine |
| T9392 |
3307-3308 |
HYPH |
denotes |
- |
| T9393 |
3308-3309 |
CD |
denotes |
8 |
| T9394 |
3309-3310 |
HYPH |
denotes |
- |
| T9395 |
3310-3311 |
NN |
denotes |
D |
| T9396 |
3311-3312 |
HYPH |
denotes |
- |
| T9397 |
3321-3332 |
NN |
denotes |
vasopressin |
| T9398 |
3333-3334 |
-LRB- |
denotes |
( |
| T9399 |
3334-3339 |
NN |
denotes |
dDAVP |
| T9400 |
3339-3340 |
: |
denotes |
; |
| T9401 |
3341-3345 |
RB |
denotes |
also |
| T9402 |
3346-3352 |
VBN |
denotes |
called |
| T9403 |
3353-3365 |
NN |
denotes |
desmopressin |
| T9404 |
3365-3366 |
-RRB- |
denotes |
) |
| T9405 |
3366-3368 |
, |
denotes |
, |
| T9406 |
3371-3372 |
DT |
denotes |
a |
| T9407 |
3380-3387 |
NN |
denotes |
agonist |
| T9408 |
3373-3379 |
JJ |
denotes |
potent |
| T9409 |
3388-3390 |
IN |
denotes |
of |
| T9410 |
3391-3396 |
NN |
denotes |
AVPR2 |
| T9411 |
3396-3397 |
. |
denotes |
. |
| T9412 |
3397-3541 |
sentence |
denotes |
When administered to wild-type mice, dDAVP leads to a dramatic increase in urine concentration, from 1,293 to 5,885 mOsm (4.6-fold; Figure 1D). |
| T9413 |
3398-3402 |
WRB |
denotes |
When |
| T9414 |
3403-3415 |
VBN |
denotes |
administered |
| T9415 |
3441-3446 |
VBZ |
denotes |
leads |
| T9416 |
3416-3418 |
IN |
denotes |
to |
| T9417 |
3419-3423 |
JJ |
denotes |
wild |
| T9418 |
3424-3428 |
NN |
denotes |
type |
| T9419 |
3423-3424 |
HYPH |
denotes |
- |
| T9420 |
3429-3433 |
NNS |
denotes |
mice |
| T9421 |
3433-3435 |
, |
denotes |
, |
| T9422 |
3435-3440 |
NN |
denotes |
dDAVP |
| T9423 |
3447-3449 |
IN |
denotes |
to |
| T9424 |
3450-3451 |
DT |
denotes |
a |
| T9425 |
3461-3469 |
NN |
denotes |
increase |
| T9426 |
3452-3460 |
JJ |
denotes |
dramatic |
| T9427 |
3470-3472 |
IN |
denotes |
in |
| T9428 |
3473-3478 |
NN |
denotes |
urine |
| T9429 |
3479-3492 |
NN |
denotes |
concentration |
| T9430 |
3492-3494 |
, |
denotes |
, |
| T9431 |
3494-3498 |
IN |
denotes |
from |
| T9432 |
3499-3504 |
CD |
denotes |
1,293 |
| T9433 |
3505-3507 |
IN |
denotes |
to |
| T9434 |
3508-3513 |
CD |
denotes |
5,885 |
| T9435 |
3514-3518 |
NN |
denotes |
mOsm |
| T9436 |
3519-3520 |
-LRB- |
denotes |
( |
| T9437 |
3530-3536 |
NN |
denotes |
Figure |
| T9438 |
3520-3528 |
RB |
denotes |
4.6-fold |
| T9439 |
3528-3529 |
: |
denotes |
; |
| T9440 |
3537-3539 |
CD |
denotes |
1D |
| T9441 |
3539-3540 |
-RRB- |
denotes |
) |
| T9442 |
3540-3541 |
. |
denotes |
. |
| T9443 |
3541-3813 |
sentence |
denotes |
With similar treatment, mutant mice concentrate their urine to a lesser but still significant extent, from 161 to 470 mOsm (2.9-fold), indicating that these animals are not only unable to concentrate their urine properly but are also defective in their response to dDAVP. |
| T9444 |
3542-3546 |
IN |
denotes |
With |
| T9445 |
3578-3589 |
VBP |
denotes |
concentrate |
| T9446 |
3547-3554 |
JJ |
denotes |
similar |
| T9447 |
3555-3564 |
NN |
denotes |
treatment |
| T9448 |
3564-3566 |
, |
denotes |
, |
| T9449 |
3566-3572 |
NN |
denotes |
mutant |
| T9450 |
3573-3577 |
NNS |
denotes |
mice |
| T9451 |
3590-3595 |
PRP$ |
denotes |
their |
| T9452 |
3596-3601 |
NN |
denotes |
urine |
| T9453 |
3602-3604 |
IN |
denotes |
to |
| T9454 |
3605-3606 |
DT |
denotes |
a |
| T9455 |
3636-3642 |
NN |
denotes |
extent |
| T9456 |
3607-3613 |
JJR |
denotes |
lesser |
| T9457 |
3614-3617 |
CC |
denotes |
but |
| T9458 |
3618-3623 |
RB |
denotes |
still |
| T9459 |
3624-3635 |
JJ |
denotes |
significant |
| T9460 |
3642-3644 |
, |
denotes |
, |
| T9461 |
3644-3648 |
IN |
denotes |
from |
| T9462 |
3649-3652 |
CD |
denotes |
161 |
| T9463 |
3653-3655 |
IN |
denotes |
to |
| T9464 |
3656-3659 |
CD |
denotes |
470 |
| T9465 |
3660-3664 |
NN |
denotes |
mOsm |
| T9466 |
3665-3666 |
-LRB- |
denotes |
( |
| T9467 |
3666-3674 |
RB |
denotes |
2.9-fold |
| T9468 |
3674-3675 |
-RRB- |
denotes |
) |
| T9469 |
3675-3677 |
, |
denotes |
, |
| T9470 |
3677-3687 |
VBG |
denotes |
indicating |
| T9471 |
3688-3692 |
IN |
denotes |
that |
| T9472 |
3707-3710 |
VBP |
denotes |
are |
| T9473 |
3693-3698 |
DT |
denotes |
these |
| T9474 |
3699-3706 |
NNS |
denotes |
animals |
| T9475 |
3711-3714 |
RB |
denotes |
not |
| T9476 |
3715-3719 |
RB |
denotes |
only |
| T9477 |
3720-3726 |
JJ |
denotes |
unable |
| T9478 |
3727-3729 |
TO |
denotes |
to |
| T9479 |
3730-3741 |
VB |
denotes |
concentrate |
| T9480 |
3742-3747 |
PRP$ |
denotes |
their |
| T9481 |
3748-3753 |
NN |
denotes |
urine |
| T9482 |
3754-3762 |
RB |
denotes |
properly |
| T9483 |
3763-3766 |
CC |
denotes |
but |
| T9484 |
3767-3770 |
VBP |
denotes |
are |
| T9485 |
3771-3775 |
RB |
denotes |
also |
| T9486 |
3776-3785 |
JJ |
denotes |
defective |
| T9487 |
3786-3788 |
IN |
denotes |
in |
| T9488 |
3789-3794 |
PRP$ |
denotes |
their |
| T9489 |
3795-3803 |
NN |
denotes |
response |
| T9490 |
3804-3806 |
IN |
denotes |
to |
| T9491 |
3807-3812 |
NN |
denotes |
dDAVP |
| T9492 |
3812-3813 |
. |
denotes |
. |
| T9493 |
3813-4012 |
sentence |
denotes |
The smaller response to dDAVP indicates some residual activity of the mutant AQP2 channel, which must be sufficient to allow survival of the individual, in contrast to the T126M knock-in mouse [18]. |
| T9494 |
3814-3817 |
DT |
denotes |
The |
| T9495 |
3826-3834 |
NN |
denotes |
response |
| T9496 |
3818-3825 |
JJR |
denotes |
smaller |
| T9497 |
3844-3853 |
VBZ |
denotes |
indicates |
| T9498 |
3835-3837 |
IN |
denotes |
to |
| T9499 |
3838-3843 |
NN |
denotes |
dDAVP |
| T9500 |
3854-3858 |
DT |
denotes |
some |
| T9501 |
3868-3876 |
NN |
denotes |
activity |
| T9502 |
3859-3867 |
JJ |
denotes |
residual |
| T9503 |
3877-3879 |
IN |
denotes |
of |
| T9504 |
3880-3883 |
DT |
denotes |
the |
| T9505 |
3896-3903 |
NN |
denotes |
channel |
| T9506 |
3884-3890 |
NN |
denotes |
mutant |
| T9507 |
3891-3895 |
NN |
denotes |
AQP2 |
| T9508 |
3903-3905 |
, |
denotes |
, |
| T9509 |
3905-3910 |
WDT |
denotes |
which |
| T9510 |
3916-3918 |
VB |
denotes |
be |
| T9511 |
3911-3915 |
MD |
denotes |
must |
| T9512 |
3919-3929 |
JJ |
denotes |
sufficient |
| T9513 |
3930-3932 |
TO |
denotes |
to |
| T9514 |
3933-3938 |
VB |
denotes |
allow |
| T9515 |
3939-3947 |
NN |
denotes |
survival |
| T9516 |
3948-3950 |
IN |
denotes |
of |
| T9517 |
3951-3954 |
DT |
denotes |
the |
| T9518 |
3955-3965 |
NN |
denotes |
individual |
| T9519 |
3965-3967 |
, |
denotes |
, |
| T9520 |
3967-3969 |
IN |
denotes |
in |
| T9521 |
3970-3978 |
NN |
denotes |
contrast |
| T9522 |
3979-3981 |
IN |
denotes |
to |
| T9523 |
3982-3985 |
DT |
denotes |
the |
| T9524 |
4001-4006 |
NN |
denotes |
mouse |
| T9525 |
3986-3991 |
NN |
denotes |
T126M |
| T9526 |
3992-3997 |
VB |
denotes |
knock |
| T9527 |
3997-3998 |
HYPH |
denotes |
- |
| T9528 |
3998-4000 |
RP |
denotes |
in |
| T9529 |
4007-4008 |
-LRB- |
denotes |
[ |
| T9530 |
4008-4010 |
CD |
denotes |
18 |
| T9531 |
4010-4011 |
-RRB- |
denotes |
] |
| T9532 |
4011-4012 |
. |
denotes |
. |
| T9533 |
4012-4270 |
sentence |
denotes |
Multiple heterozygous matings yielded 101 animals, which appeared at a ratio of 26:49:26, near the expected Mendelian wild type, heterozygote, and mutant frequencies, respectively, indicating that there is no reduced viability associated with this mutation. |
| T9534 |
4013-4021 |
JJ |
denotes |
Multiple |
| T9535 |
4035-4042 |
NNS |
denotes |
matings |
| T9536 |
4022-4034 |
JJ |
denotes |
heterozygous |
| T9537 |
4043-4050 |
VBD |
denotes |
yielded |
| T9538 |
4051-4054 |
CD |
denotes |
101 |
| T9539 |
4055-4062 |
NNS |
denotes |
animals |
| T9540 |
4062-4064 |
, |
denotes |
, |
| T9541 |
4064-4069 |
WDT |
denotes |
which |
| T9542 |
4070-4078 |
VBD |
denotes |
appeared |
| T9543 |
4079-4081 |
IN |
denotes |
at |
| T9544 |
4082-4083 |
DT |
denotes |
a |
| T9545 |
4084-4089 |
NN |
denotes |
ratio |
| T9546 |
4090-4092 |
IN |
denotes |
of |
| T9547 |
4093-4095 |
CD |
denotes |
26 |
| T9548 |
4095-4096 |
SYM |
denotes |
: |
| T9549 |
4096-4098 |
CD |
denotes |
49 |
| T9550 |
4098-4099 |
SYM |
denotes |
: |
| T9551 |
4099-4101 |
CD |
denotes |
26 |
| T9552 |
4101-4103 |
, |
denotes |
, |
| T9553 |
4103-4107 |
IN |
denotes |
near |
| T9554 |
4108-4111 |
DT |
denotes |
the |
| T9555 |
4167-4178 |
NNS |
denotes |
frequencies |
| T9556 |
4112-4120 |
VBN |
denotes |
expected |
| T9557 |
4121-4130 |
JJ |
denotes |
Mendelian |
| T9558 |
4136-4140 |
NN |
denotes |
type |
| T9559 |
4131-4135 |
JJ |
denotes |
wild |
| T9560 |
4140-4142 |
, |
denotes |
, |
| T9561 |
4142-4154 |
NN |
denotes |
heterozygote |
| T9562 |
4154-4156 |
, |
denotes |
, |
| T9563 |
4156-4159 |
CC |
denotes |
and |
| T9564 |
4160-4166 |
NN |
denotes |
mutant |
| T9565 |
4178-4180 |
, |
denotes |
, |
| T9566 |
4180-4192 |
RB |
denotes |
respectively |
| T9567 |
4192-4194 |
, |
denotes |
, |
| T9568 |
4194-4204 |
VBG |
denotes |
indicating |
| T9569 |
4205-4209 |
IN |
denotes |
that |
| T9570 |
4216-4218 |
VBZ |
denotes |
is |
| T9571 |
4210-4215 |
EX |
denotes |
there |
| T9572 |
4219-4221 |
DT |
denotes |
no |
| T9573 |
4230-4239 |
NN |
denotes |
viability |
| T9574 |
4222-4229 |
VBN |
denotes |
reduced |
| T9575 |
4240-4250 |
VBN |
denotes |
associated |
| T9576 |
4251-4255 |
IN |
denotes |
with |
| T9577 |
4256-4260 |
DT |
denotes |
this |
| T9578 |
4261-4269 |
NN |
denotes |
mutation |
| T9579 |
4269-4270 |
. |
denotes |
. |
| T9580 |
4270-4453 |
sentence |
denotes |
Other than the increased urine production and water intake, there was no overt phenotype in mutant mice, save distended kidneys, which appeared variably in adult animals (Figure 2A). |
| T9581 |
4271-4276 |
JJ |
denotes |
Other |
| T9582 |
4337-4340 |
VBD |
denotes |
was |
| T9583 |
4277-4281 |
IN |
denotes |
than |
| T9584 |
4282-4285 |
DT |
denotes |
the |
| T9585 |
4302-4312 |
NN |
denotes |
production |
| T9586 |
4286-4295 |
VBN |
denotes |
increased |
| T9587 |
4296-4301 |
NN |
denotes |
urine |
| T9588 |
4313-4316 |
CC |
denotes |
and |
| T9589 |
4317-4322 |
NN |
denotes |
water |
| T9590 |
4323-4329 |
NN |
denotes |
intake |
| T9591 |
4329-4331 |
, |
denotes |
, |
| T9592 |
4331-4336 |
EX |
denotes |
there |
| T9593 |
4341-4343 |
DT |
denotes |
no |
| T9594 |
4350-4359 |
NN |
denotes |
phenotype |
| T9595 |
4344-4349 |
JJ |
denotes |
overt |
| T9596 |
4360-4362 |
IN |
denotes |
in |
| T9597 |
4363-4369 |
NN |
denotes |
mutant |
| T9598 |
4370-4374 |
NNS |
denotes |
mice |
| T9599 |
4374-4376 |
, |
denotes |
, |
| T9600 |
4376-4380 |
IN |
denotes |
save |
| T9601 |
4381-4390 |
JJ |
denotes |
distended |
| T9602 |
4391-4398 |
NNS |
denotes |
kidneys |
| T9603 |
4398-4400 |
, |
denotes |
, |
| T9604 |
4400-4405 |
WDT |
denotes |
which |
| T9605 |
4406-4414 |
VBD |
denotes |
appeared |
| T9606 |
4415-4423 |
RB |
denotes |
variably |
| T9607 |
4424-4426 |
IN |
denotes |
in |
| T9608 |
4427-4432 |
JJ |
denotes |
adult |
| T9609 |
4433-4440 |
NNS |
denotes |
animals |
| T9610 |
4441-4442 |
-LRB- |
denotes |
( |
| T9611 |
4442-4448 |
NN |
denotes |
Figure |
| T9612 |
4449-4451 |
CD |
denotes |
2A |
| T9613 |
4451-4452 |
-RRB- |
denotes |
) |
| T9614 |
4452-4453 |
. |
denotes |
. |
| T9615 |
4453-4533 |
sentence |
denotes |
Although not specifically measured, mutant mice seem to have a normal lifespan. |
| T9616 |
4454-4462 |
IN |
denotes |
Although |
| T9617 |
4480-4488 |
VBN |
denotes |
measured |
| T9618 |
4463-4466 |
RB |
denotes |
not |
| T9619 |
4467-4479 |
RB |
denotes |
specifically |
| T9620 |
4502-4506 |
VBP |
denotes |
seem |
| T9621 |
4488-4490 |
, |
denotes |
, |
| T9622 |
4490-4496 |
NN |
denotes |
mutant |
| T9623 |
4497-4501 |
NNS |
denotes |
mice |
| T9624 |
4507-4509 |
TO |
denotes |
to |
| T9625 |
4510-4514 |
VB |
denotes |
have |
| T9626 |
4515-4516 |
DT |
denotes |
a |
| T9627 |
4524-4532 |
NN |
denotes |
lifespan |
| T9628 |
4517-4523 |
JJ |
denotes |
normal |
| T9629 |
4532-4533 |
. |
denotes |
. |
| T9630 |
4533-4617 |
sentence |
denotes |
The one animal that was followed lived to 18 mo, typical for animals in our colony. |
| T9631 |
4534-4537 |
DT |
denotes |
The |
| T9632 |
4542-4548 |
NN |
denotes |
animal |
| T9633 |
4538-4541 |
CD |
denotes |
one |
| T9634 |
4567-4572 |
VBN |
denotes |
lived |
| T9635 |
4549-4553 |
WDT |
denotes |
that |
| T9636 |
4558-4566 |
VBN |
denotes |
followed |
| T9637 |
4554-4557 |
VBD |
denotes |
was |
| T9638 |
4573-4575 |
IN |
denotes |
to |
| T9639 |
4576-4578 |
CD |
denotes |
18 |
| T9640 |
4579-4581 |
NNS |
denotes |
mo |
| T9641 |
4581-4583 |
, |
denotes |
, |
| T9642 |
4583-4590 |
JJ |
denotes |
typical |
| T9643 |
4591-4594 |
IN |
denotes |
for |
| T9644 |
4595-4602 |
NNS |
denotes |
animals |
| T9645 |
4603-4605 |
IN |
denotes |
in |
| T9646 |
4606-4609 |
PRP$ |
denotes |
our |
| T9647 |
4610-4616 |
NN |
denotes |
colony |
| T9648 |
4616-4617 |
. |
denotes |
. |
| T9649 |
4617-5615 |
sentence |
denotes |
Figure 2 Anatomy and Histology of Mouse Kidneys
(A) Gross anatomy of an affected mouse (8-mo-old male). This shows the enlargement and cystic dilatation of the renal pelvis. There is thinning of the overlying renal parenchyma imparting a translucent appearance to portions of the kidney and collecting system. The bladder is also dilated.
(B) Left kidney from mutant mouse (right) shown in (A) compared to a kidney from an age-sex matched unaffected littermate (left).
(C) Hematoxylin and eosin stained section of ureter from a mutant mouse, showing normal histology despite bloating of the kidney.
(D) Hematoxylin and eosin stained histologic section of a kidney from a 4-wk-old female mutant mouse. The mutant kidney shows marked dilatation of the renal pelvis with blunting of the papilla. There is preservation of the cortex and medulla. Aqp2F204V/F204V mice suffer from severe hydronephrosis (Figure 2A and 2B), presumably as a consequence of an inability to cope with the extreme polyuria. |
| T9650 |
5462-5471 |
NN |
denotes |
Aqp2F204V |
| T9651 |
5472-5477 |
NN |
denotes |
F204V |
| T9652 |
5471-5472 |
HYPH |
denotes |
/ |
| T9653 |
5478-5482 |
NNS |
denotes |
mice |
| T9654 |
5483-5489 |
VBP |
denotes |
suffer |
| T9655 |
5490-5494 |
IN |
denotes |
from |
| T9656 |
5495-5501 |
JJ |
denotes |
severe |
| T9657 |
5502-5516 |
NN |
denotes |
hydronephrosis |
| T9658 |
5517-5518 |
-LRB- |
denotes |
( |
| T9659 |
5525-5527 |
CD |
denotes |
2A |
| T9660 |
5518-5524 |
NN |
denotes |
Figure |
| T9661 |
5528-5531 |
CC |
denotes |
and |
| T9662 |
5532-5534 |
CD |
denotes |
2B |
| T9663 |
5534-5535 |
-RRB- |
denotes |
) |
| T9664 |
5535-5537 |
, |
denotes |
, |
| T9665 |
5537-5547 |
RB |
denotes |
presumably |
| T9666 |
5548-5550 |
IN |
denotes |
as |
| T9667 |
5551-5552 |
DT |
denotes |
a |
| T9668 |
5553-5564 |
NN |
denotes |
consequence |
| T9669 |
5565-5567 |
IN |
denotes |
of |
| T9670 |
5568-5570 |
DT |
denotes |
an |
| T9671 |
5571-5580 |
NN |
denotes |
inability |
| T9672 |
5581-5583 |
TO |
denotes |
to |
| T9673 |
5584-5588 |
VB |
denotes |
cope |
| T9674 |
5589-5593 |
IN |
denotes |
with |
| T9675 |
5594-5597 |
DT |
denotes |
the |
| T9676 |
5606-5614 |
NN |
denotes |
polyuria |
| T9677 |
5598-5605 |
JJ |
denotes |
extreme |
| T9678 |
5614-5615 |
. |
denotes |
. |
| T9679 |
5615-5757 |
sentence |
denotes |
We found distended kidneys in all Aqp2F204V/F204V mice; however, the degree of inflation was variable in affected mice and worsened with age. |
| T9680 |
5616-5618 |
PRP |
denotes |
We |
| T9681 |
5619-5624 |
VBD |
denotes |
found |
| T9682 |
5705-5708 |
VBD |
denotes |
was |
| T9683 |
5625-5634 |
JJ |
denotes |
distended |
| T9684 |
5635-5642 |
NNS |
denotes |
kidneys |
| T9685 |
5643-5645 |
IN |
denotes |
in |
| T9686 |
5646-5649 |
DT |
denotes |
all |
| T9687 |
5666-5670 |
NNS |
denotes |
mice |
| T9688 |
5650-5659 |
NN |
denotes |
Aqp2F204V |
| T9689 |
5660-5665 |
NN |
denotes |
F204V |
| T9690 |
5659-5660 |
HYPH |
denotes |
/ |
| T9691 |
5670-5671 |
: |
denotes |
; |
| T9692 |
5672-5679 |
RB |
denotes |
however |
| T9693 |
5679-5681 |
, |
denotes |
, |
| T9694 |
5681-5684 |
DT |
denotes |
the |
| T9695 |
5685-5691 |
NN |
denotes |
degree |
| T9696 |
5692-5694 |
IN |
denotes |
of |
| T9697 |
5695-5704 |
NN |
denotes |
inflation |
| T9698 |
5709-5717 |
JJ |
denotes |
variable |
| T9699 |
5718-5720 |
IN |
denotes |
in |
| T9700 |
5721-5729 |
VBN |
denotes |
affected |
| T9701 |
5730-5734 |
NNS |
denotes |
mice |
| T9702 |
5735-5738 |
CC |
denotes |
and |
| T9703 |
5739-5747 |
VBD |
denotes |
worsened |
| T9704 |
5748-5752 |
IN |
denotes |
with |
| T9705 |
5753-5756 |
NN |
denotes |
age |
| T9706 |
5756-5757 |
. |
denotes |
. |
| T9707 |
5757-5870 |
sentence |
denotes |
Severe hydronephrosis has previously been observed in double Aqp1/Aqp3 knock-out mice [17], and appears at 6 wk. |
| T9708 |
5758-5764 |
JJ |
denotes |
Severe |
| T9709 |
5765-5779 |
NN |
denotes |
hydronephrosis |
| T9710 |
5800-5808 |
VBN |
denotes |
observed |
| T9711 |
5780-5783 |
VBZ |
denotes |
has |
| T9712 |
5784-5794 |
RB |
denotes |
previously |
| T9713 |
5795-5799 |
VBN |
denotes |
been |
| T9714 |
5809-5811 |
IN |
denotes |
in |
| T9715 |
5812-5818 |
JJ |
denotes |
double |
| T9716 |
5829-5834 |
VB |
denotes |
knock |
| T9717 |
5819-5823 |
NN |
denotes |
Aqp1 |
| T9718 |
5824-5828 |
NN |
denotes |
Aqp3 |
| T9719 |
5823-5824 |
HYPH |
denotes |
/ |
| T9720 |
5839-5843 |
NNS |
denotes |
mice |
| T9721 |
5834-5835 |
HYPH |
denotes |
- |
| T9722 |
5835-5838 |
RP |
denotes |
out |
| T9723 |
5844-5845 |
-LRB- |
denotes |
[ |
| T9724 |
5845-5847 |
CD |
denotes |
17 |
| T9725 |
5847-5848 |
-RRB- |
denotes |
] |
| T9726 |
5848-5850 |
, |
denotes |
, |
| T9727 |
5850-5853 |
CC |
denotes |
and |
| T9728 |
5854-5861 |
VBZ |
denotes |
appears |
| T9729 |
5862-5864 |
IN |
denotes |
at |
| T9730 |
5865-5866 |
CD |
denotes |
6 |
| T9731 |
5867-5869 |
NNS |
denotes |
wk |
| T9732 |
5869-5870 |
. |
denotes |
. |
| T9733 |
5870-5925 |
sentence |
denotes |
Even at 4 wk, Aqp2F204V/F204V mice had hydronephrosis. |
| T9734 |
5871-5875 |
RB |
denotes |
Even |
| T9735 |
5876-5878 |
IN |
denotes |
at |
| T9736 |
5906-5909 |
VBD |
denotes |
had |
| T9737 |
5879-5880 |
CD |
denotes |
4 |
| T9738 |
5881-5883 |
NNS |
denotes |
wk |
| T9739 |
5883-5885 |
, |
denotes |
, |
| T9740 |
5885-5894 |
NN |
denotes |
Aqp2F204V |
| T9741 |
5895-5900 |
NN |
denotes |
F204V |
| T9742 |
5894-5895 |
HYPH |
denotes |
/ |
| T9743 |
5901-5905 |
NNS |
denotes |
mice |
| T9744 |
5910-5924 |
NN |
denotes |
hydronephrosis |
| T9745 |
5924-5925 |
. |
denotes |
. |
| T9746 |
5925-6078 |
sentence |
denotes |
Histologic sections from Aqp2F204V/F204V mice demonstrated marked dilatation of the renal pelvis yet normal morphology of the ureter (Figure 2C and 2D). |
| T9747 |
5926-5936 |
JJ |
denotes |
Histologic |
| T9748 |
5937-5945 |
NNS |
denotes |
sections |
| T9749 |
5972-5984 |
VBD |
denotes |
demonstrated |
| T9750 |
5946-5950 |
IN |
denotes |
from |
| T9751 |
5951-5960 |
NN |
denotes |
Aqp2F204V |
| T9752 |
5961-5966 |
NN |
denotes |
F204V |
| T9753 |
5960-5961 |
HYPH |
denotes |
/ |
| T9754 |
5967-5971 |
NNS |
denotes |
mice |
| T9755 |
5985-5991 |
JJ |
denotes |
marked |
| T9756 |
5992-6002 |
NN |
denotes |
dilatation |
| T9757 |
6003-6005 |
IN |
denotes |
of |
| T9758 |
6006-6009 |
DT |
denotes |
the |
| T9759 |
6016-6022 |
NN |
denotes |
pelvis |
| T9760 |
6010-6015 |
JJ |
denotes |
renal |
| T9761 |
6023-6026 |
CC |
denotes |
yet |
| T9762 |
6027-6033 |
JJ |
denotes |
normal |
| T9763 |
6034-6044 |
NN |
denotes |
morphology |
| T9764 |
6045-6047 |
IN |
denotes |
of |
| T9765 |
6048-6051 |
DT |
denotes |
the |
| T9766 |
6052-6058 |
NN |
denotes |
ureter |
| T9767 |
6059-6060 |
-LRB- |
denotes |
( |
| T9768 |
6067-6069 |
CD |
denotes |
2C |
| T9769 |
6060-6066 |
NN |
denotes |
Figure |
| T9770 |
6070-6073 |
CC |
denotes |
and |
| T9771 |
6074-6076 |
CD |
denotes |
2D |
| T9772 |
6076-6077 |
-RRB- |
denotes |
) |
| T9773 |
6077-6078 |
. |
denotes |
. |
| T9774 |
6078-6155 |
sentence |
denotes |
In particular, the muscularis propria was neither hypertrophied nor thinned. |
| T9775 |
6079-6081 |
IN |
denotes |
In |
| T9776 |
6117-6120 |
VBD |
denotes |
was |
| T9777 |
6082-6092 |
JJ |
denotes |
particular |
| T9778 |
6092-6094 |
, |
denotes |
, |
| T9779 |
6094-6097 |
DT |
denotes |
the |
| T9780 |
6109-6116 |
NN |
denotes |
propria |
| T9781 |
6098-6108 |
NN |
denotes |
muscularis |
| T9782 |
6121-6128 |
CC |
denotes |
neither |
| T9783 |
6129-6142 |
JJ |
denotes |
hypertrophied |
| T9784 |
6143-6146 |
CC |
denotes |
nor |
| T9785 |
6147-6154 |
JJ |
denotes |
thinned |
| T9786 |
6154-6155 |
. |
denotes |
. |
| T9787 |
6155-6274 |
sentence |
denotes |
There was the normal festooned appearance of the urothelium, and this transitional epithelium was of normal thickness. |
| T9788 |
6156-6161 |
EX |
denotes |
There |
| T9789 |
6162-6165 |
VBD |
denotes |
was |
| T9790 |
6166-6169 |
DT |
denotes |
the |
| T9791 |
6187-6197 |
NN |
denotes |
appearance |
| T9792 |
6170-6176 |
JJ |
denotes |
normal |
| T9793 |
6177-6186 |
VBN |
denotes |
festooned |
| T9794 |
6198-6200 |
IN |
denotes |
of |
| T9795 |
6201-6204 |
DT |
denotes |
the |
| T9796 |
6205-6215 |
NN |
denotes |
urothelium |
| T9797 |
6215-6217 |
, |
denotes |
, |
| T9798 |
6217-6220 |
CC |
denotes |
and |
| T9799 |
6221-6225 |
DT |
denotes |
this |
| T9800 |
6239-6249 |
NN |
denotes |
epithelium |
| T9801 |
6226-6238 |
JJ |
denotes |
transitional |
| T9802 |
6250-6253 |
VBD |
denotes |
was |
| T9803 |
6254-6256 |
IN |
denotes |
of |
| T9804 |
6257-6263 |
JJ |
denotes |
normal |
| T9805 |
6264-6273 |
NN |
denotes |
thickness |
| T9806 |
6273-6274 |
. |
denotes |
. |
| T9807 |
6274-6355 |
sentence |
denotes |
There was thinning of the kidney as measured from renal capsule to renal pelvis. |
| T9808 |
6275-6280 |
EX |
denotes |
There |
| T9809 |
6281-6284 |
VBD |
denotes |
was |
| T9810 |
6285-6293 |
NN |
denotes |
thinning |
| T9811 |
6294-6296 |
IN |
denotes |
of |
| T9812 |
6297-6300 |
DT |
denotes |
the |
| T9813 |
6301-6307 |
NN |
denotes |
kidney |
| T9814 |
6308-6310 |
IN |
denotes |
as |
| T9815 |
6311-6319 |
VBN |
denotes |
measured |
| T9816 |
6320-6324 |
IN |
denotes |
from |
| T9817 |
6325-6330 |
JJ |
denotes |
renal |
| T9818 |
6331-6338 |
NN |
denotes |
capsule |
| T9819 |
6339-6341 |
IN |
denotes |
to |
| T9820 |
6342-6347 |
JJ |
denotes |
renal |
| T9821 |
6348-6354 |
NN |
denotes |
pelvis |
| T9822 |
6354-6355 |
. |
denotes |
. |
| T9823 |
6355-6465 |
sentence |
denotes |
However, the morphologic features of the glomeruli and proximal/distal tubules were unremarkable (Figure 2D). |
| T9824 |
6356-6363 |
RB |
denotes |
However |
| T9825 |
6435-6439 |
VBD |
denotes |
were |
| T9826 |
6363-6365 |
, |
denotes |
, |
| T9827 |
6365-6368 |
DT |
denotes |
the |
| T9828 |
6381-6389 |
NNS |
denotes |
features |
| T9829 |
6369-6380 |
JJ |
denotes |
morphologic |
| T9830 |
6390-6392 |
IN |
denotes |
of |
| T9831 |
6393-6396 |
DT |
denotes |
the |
| T9832 |
6397-6406 |
NNS |
denotes |
glomeruli |
| T9833 |
6407-6410 |
CC |
denotes |
and |
| T9834 |
6411-6419 |
JJ |
denotes |
proximal |
| T9835 |
6420-6426 |
JJ |
denotes |
distal |
| T9836 |
6419-6420 |
HYPH |
denotes |
/ |
| T9837 |
6427-6434 |
NNS |
denotes |
tubules |
| T9838 |
6440-6452 |
JJ |
denotes |
unremarkable |
| T9839 |
6453-6454 |
-LRB- |
denotes |
( |
| T9840 |
6454-6460 |
NN |
denotes |
Figure |
| T9841 |
6461-6463 |
CD |
denotes |
2D |
| T9842 |
6463-6464 |
-RRB- |
denotes |
) |
| T9843 |
6464-6465 |
. |
denotes |
. |
| T9844 |
6465-6614 |
sentence |
denotes |
As shown previously [18,22], immunoblotting revealed three different forms of AQP2, due to different degrees and forms of glycosylation (Figure 3A). |
| T9845 |
6466-6468 |
IN |
denotes |
As |
| T9846 |
6469-6474 |
VBN |
denotes |
shown |
| T9847 |
6510-6518 |
VBD |
denotes |
revealed |
| T9848 |
6475-6485 |
RB |
denotes |
previously |
| T9849 |
6486-6487 |
-LRB- |
denotes |
[ |
| T9850 |
6490-6492 |
CD |
denotes |
22 |
| T9851 |
6487-6489 |
CD |
denotes |
18 |
| T9852 |
6489-6490 |
, |
denotes |
, |
| T9853 |
6492-6493 |
-RRB- |
denotes |
] |
| T9854 |
6493-6495 |
, |
denotes |
, |
| T9855 |
6495-6509 |
NN |
denotes |
immunoblotting |
| T9856 |
6519-6524 |
CD |
denotes |
three |
| T9857 |
6535-6540 |
NNS |
denotes |
forms |
| T9858 |
6525-6534 |
JJ |
denotes |
different |
| T9859 |
6541-6543 |
IN |
denotes |
of |
| T9860 |
6544-6548 |
NN |
denotes |
AQP2 |
| T9861 |
6548-6550 |
, |
denotes |
, |
| T9862 |
6550-6553 |
IN |
denotes |
due |
| T9863 |
6554-6556 |
IN |
denotes |
to |
| T9864 |
6557-6566 |
JJ |
denotes |
different |
| T9865 |
6567-6574 |
NNS |
denotes |
degrees |
| T9866 |
6575-6578 |
CC |
denotes |
and |
| T9867 |
6579-6584 |
NNS |
denotes |
forms |
| T9868 |
6585-6587 |
IN |
denotes |
of |
| T9869 |
6588-6601 |
NN |
denotes |
glycosylation |
| T9870 |
6602-6603 |
-LRB- |
denotes |
( |
| T9871 |
6603-6609 |
NN |
denotes |
Figure |
| T9872 |
6610-6612 |
CD |
denotes |
3A |
| T9873 |
6612-6613 |
-RRB- |
denotes |
) |
| T9874 |
6613-6614 |
. |
denotes |
. |
| T9875 |
6614-6778 |
sentence |
denotes |
Previous reports have demonstrated that nonglycosylated protein appears as a 29 kDa band, while complex glycosylated protein runs as a smear between 35 and 45 kDa. |
| T9876 |
6615-6623 |
JJ |
denotes |
Previous |
| T9877 |
6624-6631 |
NNS |
denotes |
reports |
| T9878 |
6637-6649 |
VBN |
denotes |
demonstrated |
| T9879 |
6632-6636 |
VBP |
denotes |
have |
| T9880 |
6650-6654 |
IN |
denotes |
that |
| T9881 |
6679-6686 |
VBZ |
denotes |
appears |
| T9882 |
6655-6670 |
JJ |
denotes |
nonglycosylated |
| T9883 |
6671-6678 |
NN |
denotes |
protein |
| T9884 |
6687-6689 |
IN |
denotes |
as |
| T9885 |
6690-6691 |
DT |
denotes |
a |
| T9886 |
6699-6703 |
NN |
denotes |
band |
| T9887 |
6692-6694 |
CD |
denotes |
29 |
| T9888 |
6695-6698 |
NN |
denotes |
kDa |
| T9889 |
6703-6705 |
, |
denotes |
, |
| T9890 |
6705-6710 |
IN |
denotes |
while |
| T9891 |
6740-6744 |
VBZ |
denotes |
runs |
| T9892 |
6711-6718 |
JJ |
denotes |
complex |
| T9893 |
6732-6739 |
NN |
denotes |
protein |
| T9894 |
6719-6731 |
VBN |
denotes |
glycosylated |
| T9895 |
6745-6747 |
IN |
denotes |
as |
| T9896 |
6748-6749 |
DT |
denotes |
a |
| T9897 |
6750-6755 |
NN |
denotes |
smear |
| T9898 |
6756-6763 |
IN |
denotes |
between |
| T9899 |
6764-6766 |
CD |
denotes |
35 |
| T9900 |
6774-6777 |
NN |
denotes |
kDa |
| T9901 |
6767-6770 |
CC |
denotes |
and |
| T9902 |
6771-6773 |
CD |
denotes |
45 |
| T9903 |
6777-6778 |
. |
denotes |
. |
| T9904 |
6778-6930 |
sentence |
denotes |
A short-lived intermediate form of 31 kDa representing core, high-mannose glycosylation of AQP2 is apparent from pulse-chase labeling experiments [22]. |
| T9905 |
6779-6780 |
DT |
denotes |
A |
| T9906 |
6806-6810 |
NN |
denotes |
form |
| T9907 |
6781-6786 |
JJ |
denotes |
short |
| T9908 |
6787-6792 |
JJ |
denotes |
lived |
| T9909 |
6786-6787 |
HYPH |
denotes |
- |
| T9910 |
6793-6805 |
JJ |
denotes |
intermediate |
| T9911 |
6875-6877 |
VBZ |
denotes |
is |
| T9912 |
6811-6813 |
IN |
denotes |
of |
| T9913 |
6814-6816 |
CD |
denotes |
31 |
| T9914 |
6817-6820 |
NN |
denotes |
kDa |
| T9915 |
6821-6833 |
VBG |
denotes |
representing |
| T9916 |
6834-6838 |
NN |
denotes |
core |
| T9917 |
6853-6866 |
NN |
denotes |
glycosylation |
| T9918 |
6838-6840 |
, |
denotes |
, |
| T9919 |
6840-6844 |
JJ |
denotes |
high |
| T9920 |
6845-6852 |
NN |
denotes |
mannose |
| T9921 |
6844-6845 |
HYPH |
denotes |
- |
| T9922 |
6867-6869 |
IN |
denotes |
of |
| T9923 |
6870-6874 |
NN |
denotes |
AQP2 |
| T9924 |
6878-6886 |
JJ |
denotes |
apparent |
| T9925 |
6887-6891 |
IN |
denotes |
from |
| T9926 |
6892-6897 |
NN |
denotes |
pulse |
| T9927 |
6898-6903 |
NN |
denotes |
chase |
| T9928 |
6897-6898 |
HYPH |
denotes |
- |
| T9929 |
6904-6912 |
NN |
denotes |
labeling |
| T9930 |
6913-6924 |
NNS |
denotes |
experiments |
| T9931 |
6925-6926 |
-LRB- |
denotes |
[ |
| T9932 |
6926-6928 |
CD |
denotes |
22 |
| T9933 |
6928-6929 |
-RRB- |
denotes |
] |
| T9934 |
6929-6930 |
. |
denotes |
. |
| T9935 |
6930-7177 |
sentence |
denotes |
Compared to that from the kidneys of wild-type animals, AQP2 from mutant animals was reduced in both the high molecular weight, diffuse form and the lowest molecular weight form, but enriched in the intermediate molecular weight form (Figure 3A). |
| T9936 |
6931-6939 |
VBN |
denotes |
Compared |
| T9937 |
7016-7023 |
VBN |
denotes |
reduced |
| T9938 |
6940-6942 |
IN |
denotes |
to |
| T9939 |
6943-6947 |
DT |
denotes |
that |
| T9940 |
6948-6952 |
IN |
denotes |
from |
| T9941 |
6953-6956 |
DT |
denotes |
the |
| T9942 |
6957-6964 |
NNS |
denotes |
kidneys |
| T9943 |
6965-6967 |
IN |
denotes |
of |
| T9944 |
6968-6972 |
JJ |
denotes |
wild |
| T9945 |
6973-6977 |
NN |
denotes |
type |
| T9946 |
6972-6973 |
HYPH |
denotes |
- |
| T9947 |
6978-6985 |
NNS |
denotes |
animals |
| T9948 |
6985-6987 |
, |
denotes |
, |
| T9949 |
6987-6991 |
NN |
denotes |
AQP2 |
| T9950 |
6992-6996 |
IN |
denotes |
from |
| T9951 |
6997-7003 |
NN |
denotes |
mutant |
| T9952 |
7004-7011 |
NNS |
denotes |
animals |
| T9953 |
7012-7015 |
VBD |
denotes |
was |
| T9954 |
7024-7026 |
IN |
denotes |
in |
| T9955 |
7027-7031 |
CC |
denotes |
both |
| T9956 |
7067-7071 |
NN |
denotes |
form |
| T9957 |
7032-7035 |
DT |
denotes |
the |
| T9958 |
7036-7040 |
JJ |
denotes |
high |
| T9959 |
7051-7057 |
NN |
denotes |
weight |
| T9960 |
7041-7050 |
JJ |
denotes |
molecular |
| T9961 |
7057-7059 |
, |
denotes |
, |
| T9962 |
7059-7066 |
JJ |
denotes |
diffuse |
| T9963 |
7072-7075 |
CC |
denotes |
and |
| T9964 |
7076-7079 |
DT |
denotes |
the |
| T9965 |
7104-7108 |
NN |
denotes |
form |
| T9966 |
7080-7086 |
JJS |
denotes |
lowest |
| T9967 |
7097-7103 |
NN |
denotes |
weight |
| T9968 |
7087-7096 |
JJ |
denotes |
molecular |
| T9969 |
7108-7110 |
, |
denotes |
, |
| T9970 |
7110-7113 |
CC |
denotes |
but |
| T9971 |
7114-7122 |
VBN |
denotes |
enriched |
| T9972 |
7123-7125 |
IN |
denotes |
in |
| T9973 |
7126-7129 |
DT |
denotes |
the |
| T9974 |
7160-7164 |
NN |
denotes |
form |
| T9975 |
7130-7142 |
JJ |
denotes |
intermediate |
| T9976 |
7153-7159 |
NN |
denotes |
weight |
| T9977 |
7143-7152 |
JJ |
denotes |
molecular |
| T9978 |
7165-7166 |
-LRB- |
denotes |
( |
| T9979 |
7166-7172 |
NN |
denotes |
Figure |
| T9980 |
7173-7175 |
CD |
denotes |
3A |
| T9981 |
7175-7176 |
-RRB- |
denotes |
) |
| T9982 |
7176-7177 |
. |
denotes |
. |
| T9983 |
7177-7246 |
sentence |
denotes |
Heterozygous animals showed intermediate amounts of all three forms. |
| T9984 |
7178-7190 |
JJ |
denotes |
Heterozygous |
| T9985 |
7191-7198 |
NNS |
denotes |
animals |
| T9986 |
7199-7205 |
VBD |
denotes |
showed |
| T9987 |
7206-7218 |
JJ |
denotes |
intermediate |
| T9988 |
7219-7226 |
NNS |
denotes |
amounts |
| T9989 |
7227-7229 |
IN |
denotes |
of |
| T9990 |
7230-7233 |
DT |
denotes |
all |
| T9991 |
7240-7245 |
NNS |
denotes |
forms |
| T9992 |
7234-7239 |
CD |
denotes |
three |
| T9993 |
7245-7246 |
. |
denotes |
. |
| T9994 |
7246-7415 |
sentence |
denotes |
The nature of these glycosylated forms was revealed by digestion with endoglycosidase H, which specifically cleaves mannose-rich carbohydrate from the protein backbone. |
| T9995 |
7247-7250 |
DT |
denotes |
The |
| T9996 |
7251-7257 |
NN |
denotes |
nature |
| T9997 |
7290-7298 |
VBN |
denotes |
revealed |
| T9998 |
7258-7260 |
IN |
denotes |
of |
| T9999 |
7261-7266 |
DT |
denotes |
these |
| T10000 |
7280-7285 |
NNS |
denotes |
forms |
| T10001 |
7267-7279 |
VBN |
denotes |
glycosylated |
| T10002 |
7286-7289 |
VBD |
denotes |
was |
| T10003 |
7299-7301 |
IN |
denotes |
by |
| T10004 |
7302-7311 |
NN |
denotes |
digestion |
| T10005 |
7312-7316 |
IN |
denotes |
with |
| T10006 |
7317-7332 |
NN |
denotes |
endoglycosidase |
| T10007 |
7333-7334 |
NN |
denotes |
H |
| T10008 |
7334-7336 |
, |
denotes |
, |
| T10009 |
7336-7341 |
WDT |
denotes |
which |
| T10010 |
7355-7362 |
VBZ |
denotes |
cleaves |
| T10011 |
7342-7354 |
RB |
denotes |
specifically |
| T10012 |
7363-7370 |
NN |
denotes |
mannose |
| T10013 |
7371-7375 |
JJ |
denotes |
rich |
| T10014 |
7370-7371 |
HYPH |
denotes |
- |
| T10015 |
7376-7388 |
NN |
denotes |
carbohydrate |
| T10016 |
7389-7393 |
IN |
denotes |
from |
| T10017 |
7394-7397 |
DT |
denotes |
the |
| T10018 |
7406-7414 |
NN |
denotes |
backbone |
| T10019 |
7398-7405 |
NN |
denotes |
protein |
| T10020 |
7414-7415 |
. |
denotes |
. |
| T10021 |
7415-7578 |
sentence |
denotes |
Treatment of endogenous AQP2 from kidneys of wild-type, heterozygous, and mutant animals specifically affected the intermediate molecular weight form (Figure 3B). |
| T10022 |
7416-7425 |
NN |
denotes |
Treatment |
| T10023 |
7518-7526 |
VBD |
denotes |
affected |
| T10024 |
7426-7428 |
IN |
denotes |
of |
| T10025 |
7429-7439 |
JJ |
denotes |
endogenous |
| T10026 |
7440-7444 |
NN |
denotes |
AQP2 |
| T10027 |
7445-7449 |
IN |
denotes |
from |
| T10028 |
7450-7457 |
NNS |
denotes |
kidneys |
| T10029 |
7458-7460 |
IN |
denotes |
of |
| T10030 |
7461-7465 |
JJ |
denotes |
wild |
| T10031 |
7466-7470 |
NN |
denotes |
type |
| T10032 |
7465-7466 |
HYPH |
denotes |
- |
| T10033 |
7497-7504 |
NNS |
denotes |
animals |
| T10034 |
7470-7472 |
, |
denotes |
, |
| T10035 |
7472-7484 |
JJ |
denotes |
heterozygous |
| T10036 |
7484-7486 |
, |
denotes |
, |
| T10037 |
7486-7489 |
CC |
denotes |
and |
| T10038 |
7490-7496 |
JJ |
denotes |
mutant |
| T10039 |
7505-7517 |
RB |
denotes |
specifically |
| T10040 |
7527-7530 |
DT |
denotes |
the |
| T10041 |
7561-7565 |
NN |
denotes |
form |
| T10042 |
7531-7543 |
JJ |
denotes |
intermediate |
| T10043 |
7554-7560 |
NN |
denotes |
weight |
| T10044 |
7544-7553 |
JJ |
denotes |
molecular |
| T10045 |
7566-7567 |
-LRB- |
denotes |
( |
| T10046 |
7567-7573 |
NN |
denotes |
Figure |
| T10047 |
7574-7576 |
CD |
denotes |
3B |
| T10048 |
7576-7577 |
-RRB- |
denotes |
) |
| T10049 |
7577-7578 |
. |
denotes |
. |
| T10050 |
7578-7787 |
sentence |
denotes |
The presence of some mature glycosylated proteins (35–45 kDa) in Aqp2F204V/F204V mice presumably permits their survival compared to Aqp2T126M/T126M mice, and is consistent with a diminished response to dDAVP. |
| T10051 |
7579-7582 |
DT |
denotes |
The |
| T10052 |
7583-7591 |
NN |
denotes |
presence |
| T10053 |
7676-7683 |
VBZ |
denotes |
permits |
| T10054 |
7592-7594 |
IN |
denotes |
of |
| T10055 |
7595-7599 |
DT |
denotes |
some |
| T10056 |
7620-7628 |
NN |
denotes |
proteins |
| T10057 |
7600-7606 |
JJ |
denotes |
mature |
| T10058 |
7607-7619 |
VBN |
denotes |
glycosylated |
| T10059 |
7629-7630 |
-LRB- |
denotes |
( |
| T10060 |
7636-7639 |
NN |
denotes |
kDa |
| T10061 |
7630-7632 |
CD |
denotes |
35 |
| T10062 |
7633-7635 |
CD |
denotes |
45 |
| T10063 |
7632-7633 |
SYM |
denotes |
– |
| T10064 |
7639-7640 |
-RRB- |
denotes |
) |
| T10065 |
7641-7643 |
IN |
denotes |
in |
| T10066 |
7644-7653 |
NN |
denotes |
Aqp2F204V |
| T10067 |
7654-7659 |
NN |
denotes |
F204V |
| T10068 |
7653-7654 |
HYPH |
denotes |
/ |
| T10069 |
7660-7664 |
NNS |
denotes |
mice |
| T10070 |
7665-7675 |
RB |
denotes |
presumably |
| T10071 |
7684-7689 |
PRP$ |
denotes |
their |
| T10072 |
7690-7698 |
NN |
denotes |
survival |
| T10073 |
7699-7707 |
VBN |
denotes |
compared |
| T10074 |
7708-7710 |
IN |
denotes |
to |
| T10075 |
7711-7720 |
NN |
denotes |
Aqp2T126M |
| T10076 |
7721-7726 |
NN |
denotes |
T126M |
| T10077 |
7720-7721 |
HYPH |
denotes |
/ |
| T10078 |
7727-7731 |
NNS |
denotes |
mice |
| T10079 |
7731-7733 |
, |
denotes |
, |
| T10080 |
7733-7736 |
CC |
denotes |
and |
| T10081 |
7737-7739 |
VBZ |
denotes |
is |
| T10082 |
7740-7750 |
JJ |
denotes |
consistent |
| T10083 |
7751-7755 |
IN |
denotes |
with |
| T10084 |
7756-7757 |
DT |
denotes |
a |
| T10085 |
7769-7777 |
NN |
denotes |
response |
| T10086 |
7758-7768 |
JJ |
denotes |
diminished |
| T10087 |
7778-7780 |
IN |
denotes |
to |
| T10088 |
7781-7786 |
NN |
denotes |
dDAVP |
| T10089 |
7786-7787 |
. |
denotes |
. |
| T10090 |
7787-8441 |
sentence |
denotes |
Figure 3 Immunoblot Analyses of AQP2 from Mouse Kidneys
(A) Western blot analyses of total kidney membranes from littermate mice. An intermediate form of AQP2 at 31 kDa was identified in kidney membranes from a mutant mouse (Mut) and partially in a heterozygous mouse (Het).
(B) Total kidney membranes were subjected to endoglycosidase H treatment (Endo H) prior to Western blotting. High-mannose (h.m.) glycosylated proteins that have not exited the ER are sensitive to endoglycosidase H digestion. In humans, recessive alleles of Aqp2 are postulated to cause NDI because they do not properly translocate to the apical cell surface in response to AVP. |
| T10091 |
8289-8291 |
IN |
denotes |
In |
| T10092 |
8330-8340 |
VBN |
denotes |
postulated |
| T10093 |
8292-8298 |
NNS |
denotes |
humans |
| T10094 |
8298-8300 |
, |
denotes |
, |
| T10095 |
8300-8309 |
JJ |
denotes |
recessive |
| T10096 |
8310-8317 |
NNS |
denotes |
alleles |
| T10097 |
8318-8320 |
IN |
denotes |
of |
| T10098 |
8321-8325 |
NN |
denotes |
Aqp2 |
| T10099 |
8326-8329 |
VBP |
denotes |
are |
| T10100 |
8341-8343 |
TO |
denotes |
to |
| T10101 |
8344-8349 |
VB |
denotes |
cause |
| T10102 |
8350-8353 |
NN |
denotes |
NDI |
| T10103 |
8354-8361 |
IN |
denotes |
because |
| T10104 |
8383-8394 |
VB |
denotes |
translocate |
| T10105 |
8362-8366 |
PRP |
denotes |
they |
| T10106 |
8367-8369 |
VBP |
denotes |
do |
| T10107 |
8370-8373 |
RB |
denotes |
not |
| T10108 |
8374-8382 |
RB |
denotes |
properly |
| T10109 |
8395-8397 |
IN |
denotes |
to |
| T10110 |
8398-8401 |
DT |
denotes |
the |
| T10111 |
8414-8421 |
NN |
denotes |
surface |
| T10112 |
8402-8408 |
JJ |
denotes |
apical |
| T10113 |
8409-8413 |
NN |
denotes |
cell |
| T10114 |
8422-8424 |
IN |
denotes |
in |
| T10115 |
8425-8433 |
NN |
denotes |
response |
| T10116 |
8434-8436 |
IN |
denotes |
to |
| T10117 |
8437-8440 |
NN |
denotes |
AVP |
| T10118 |
8440-8441 |
. |
denotes |
. |
| T10119 |
8441-8599 |
sentence |
denotes |
This postulate comes solely from in vitro studies in which mutant Aqp2 cDNAs corresponding to human disease mutations are transfected into kidney cell lines. |
| T10120 |
8442-8446 |
DT |
denotes |
This |
| T10121 |
8447-8456 |
NN |
denotes |
postulate |
| T10122 |
8457-8462 |
VBZ |
denotes |
comes |
| T10123 |
8463-8469 |
RB |
denotes |
solely |
| T10124 |
8470-8474 |
IN |
denotes |
from |
| T10125 |
8475-8477 |
FW |
denotes |
in |
| T10126 |
8478-8483 |
FW |
denotes |
vitro |
| T10127 |
8484-8491 |
NNS |
denotes |
studies |
| T10128 |
8492-8494 |
IN |
denotes |
in |
| T10129 |
8564-8575 |
VBN |
denotes |
transfected |
| T10130 |
8495-8500 |
WDT |
denotes |
which |
| T10131 |
8501-8507 |
NN |
denotes |
mutant |
| T10132 |
8513-8518 |
NNS |
denotes |
cDNAs |
| T10133 |
8508-8512 |
NN |
denotes |
Aqp2 |
| T10134 |
8519-8532 |
VBG |
denotes |
corresponding |
| T10135 |
8533-8535 |
IN |
denotes |
to |
| T10136 |
8536-8541 |
JJ |
denotes |
human |
| T10137 |
8550-8559 |
NNS |
denotes |
mutations |
| T10138 |
8542-8549 |
NN |
denotes |
disease |
| T10139 |
8560-8563 |
VBP |
denotes |
are |
| T10140 |
8576-8580 |
IN |
denotes |
into |
| T10141 |
8581-8587 |
NN |
denotes |
kidney |
| T10142 |
8593-8598 |
NNS |
denotes |
lines |
| T10143 |
8588-8592 |
NN |
denotes |
cell |
| T10144 |
8598-8599 |
. |
denotes |
. |
| T10145 |
8599-8718 |
sentence |
denotes |
In general, such recessive alleles, when visualized immunocytochemically, fail to localize to AVP-responsive vesicles. |
| T10146 |
8600-8602 |
IN |
denotes |
In |
| T10147 |
8674-8678 |
VBP |
denotes |
fail |
| T10148 |
8603-8610 |
JJ |
denotes |
general |
| T10149 |
8610-8612 |
, |
denotes |
, |
| T10150 |
8612-8616 |
JJ |
denotes |
such |
| T10151 |
8627-8634 |
NNS |
denotes |
alleles |
| T10152 |
8617-8626 |
JJ |
denotes |
recessive |
| T10153 |
8634-8636 |
, |
denotes |
, |
| T10154 |
8636-8640 |
WRB |
denotes |
when |
| T10155 |
8641-8651 |
VBN |
denotes |
visualized |
| T10156 |
8652-8672 |
RB |
denotes |
immunocytochemically |
| T10157 |
8672-8674 |
, |
denotes |
, |
| T10158 |
8679-8681 |
TO |
denotes |
to |
| T10159 |
8682-8690 |
VB |
denotes |
localize |
| T10160 |
8691-8693 |
IN |
denotes |
to |
| T10161 |
8694-8697 |
NN |
denotes |
AVP |
| T10162 |
8698-8708 |
JJ |
denotes |
responsive |
| T10163 |
8697-8698 |
HYPH |
denotes |
- |
| T10164 |
8709-8717 |
NNS |
denotes |
vesicles |
| T10165 |
8717-8718 |
. |
denotes |
. |
| T10166 |
8718-8778 |
sentence |
denotes |
Rather, they get trapped in the endoplasmic reticulum (ER). |
| T10167 |
8719-8725 |
RB |
denotes |
Rather |
| T10168 |
8736-8743 |
VBN |
denotes |
trapped |
| T10169 |
8725-8727 |
, |
denotes |
, |
| T10170 |
8727-8731 |
PRP |
denotes |
they |
| T10171 |
8732-8735 |
VBP |
denotes |
get |
| T10172 |
8744-8746 |
IN |
denotes |
in |
| T10173 |
8747-8750 |
DT |
denotes |
the |
| T10174 |
8763-8772 |
NN |
denotes |
reticulum |
| T10175 |
8751-8762 |
JJ |
denotes |
endoplasmic |
| T10176 |
8773-8774 |
-LRB- |
denotes |
( |
| T10177 |
8774-8776 |
NN |
denotes |
ER |
| T10178 |
8776-8777 |
-RRB- |
denotes |
) |
| T10179 |
8777-8778 |
. |
denotes |
. |
| T10180 |
8778-8875 |
sentence |
denotes |
Our mouse model of NDI affords the first opportunity to test this hypothesis in a mature animal. |
| T10181 |
8779-8782 |
PRP$ |
denotes |
Our |
| T10182 |
8789-8794 |
NN |
denotes |
model |
| T10183 |
8783-8788 |
NN |
denotes |
mouse |
| T10184 |
8802-8809 |
VBZ |
denotes |
affords |
| T10185 |
8795-8797 |
IN |
denotes |
of |
| T10186 |
8798-8801 |
NN |
denotes |
NDI |
| T10187 |
8810-8813 |
DT |
denotes |
the |
| T10188 |
8820-8831 |
NN |
denotes |
opportunity |
| T10189 |
8814-8819 |
JJ |
denotes |
first |
| T10190 |
8832-8834 |
TO |
denotes |
to |
| T10191 |
8835-8839 |
VB |
denotes |
test |
| T10192 |
8840-8844 |
DT |
denotes |
this |
| T10193 |
8845-8855 |
NN |
denotes |
hypothesis |
| T10194 |
8856-8858 |
IN |
denotes |
in |
| T10195 |
8859-8860 |
DT |
denotes |
a |
| T10196 |
8868-8874 |
NN |
denotes |
animal |
| T10197 |
8861-8867 |
JJ |
denotes |
mature |
| T10198 |
8874-8875 |
. |
denotes |
. |
| T10199 |
8875-9045 |
sentence |
denotes |
As shown in Figure 4A (top row of photomicrographs), AQP2 (stained red) normally localized to the subapical region of collecting duct cells in kidneys of wild-type mice. |
| T10200 |
8876-8878 |
IN |
denotes |
As |
| T10201 |
8879-8884 |
VBN |
denotes |
shown |
| T10202 |
8957-8966 |
VBD |
denotes |
localized |
| T10203 |
8885-8887 |
IN |
denotes |
in |
| T10204 |
8888-8894 |
NN |
denotes |
Figure |
| T10205 |
8895-8897 |
CD |
denotes |
4A |
| T10206 |
8898-8899 |
-LRB- |
denotes |
( |
| T10207 |
8903-8906 |
NN |
denotes |
row |
| T10208 |
8899-8902 |
JJ |
denotes |
top |
| T10209 |
8907-8909 |
IN |
denotes |
of |
| T10210 |
8910-8926 |
NNS |
denotes |
photomicrographs |
| T10211 |
8926-8927 |
-RRB- |
denotes |
) |
| T10212 |
8927-8929 |
, |
denotes |
, |
| T10213 |
8929-8933 |
NN |
denotes |
AQP2 |
| T10214 |
8934-8935 |
-LRB- |
denotes |
( |
| T10215 |
8935-8942 |
VBN |
denotes |
stained |
| T10216 |
8943-8946 |
JJ |
denotes |
red |
| T10217 |
8946-8947 |
-RRB- |
denotes |
) |
| T10218 |
8948-8956 |
RB |
denotes |
normally |
| T10219 |
8967-8969 |
IN |
denotes |
to |
| T10220 |
8970-8973 |
DT |
denotes |
the |
| T10221 |
8984-8990 |
NN |
denotes |
region |
| T10222 |
8974-8983 |
JJ |
denotes |
subapical |
| T10223 |
8991-8993 |
IN |
denotes |
of |
| T10224 |
8994-9004 |
VBG |
denotes |
collecting |
| T10225 |
9005-9009 |
NN |
denotes |
duct |
| T10226 |
9010-9015 |
NNS |
denotes |
cells |
| T10227 |
9016-9018 |
IN |
denotes |
in |
| T10228 |
9019-9026 |
NNS |
denotes |
kidneys |
| T10229 |
9027-9029 |
IN |
denotes |
of |
| T10230 |
9030-9034 |
JJ |
denotes |
wild |
| T10231 |
9035-9039 |
NN |
denotes |
type |
| T10232 |
9034-9035 |
HYPH |
denotes |
- |
| T10233 |
9040-9044 |
NNS |
denotes |
mice |
| T10234 |
9044-9045 |
. |
denotes |
. |
| T10235 |
9045-9145 |
sentence |
denotes |
Upon stimulation with dDAVP, AQP2 translocated to or near the cell surface (Figure 4A, second row). |
| T10236 |
9046-9050 |
IN |
denotes |
Upon |
| T10237 |
9080-9092 |
VBD |
denotes |
translocated |
| T10238 |
9051-9062 |
NN |
denotes |
stimulation |
| T10239 |
9063-9067 |
IN |
denotes |
with |
| T10240 |
9068-9073 |
NN |
denotes |
dDAVP |
| T10241 |
9073-9075 |
, |
denotes |
, |
| T10242 |
9075-9079 |
NN |
denotes |
AQP2 |
| T10243 |
9093-9095 |
IN |
denotes |
to |
| T10244 |
9096-9098 |
CC |
denotes |
or |
| T10245 |
9099-9103 |
IN |
denotes |
near |
| T10246 |
9104-9107 |
DT |
denotes |
the |
| T10247 |
9113-9120 |
NN |
denotes |
surface |
| T10248 |
9108-9112 |
NN |
denotes |
cell |
| T10249 |
9121-9122 |
-LRB- |
denotes |
( |
| T10250 |
9140-9143 |
NN |
denotes |
row |
| T10251 |
9122-9128 |
NN |
denotes |
Figure |
| T10252 |
9129-9131 |
CD |
denotes |
4A |
| T10253 |
9131-9133 |
, |
denotes |
, |
| T10254 |
9133-9139 |
JJ |
denotes |
second |
| T10255 |
9143-9144 |
-RRB- |
denotes |
) |
| T10256 |
9144-9145 |
. |
denotes |
. |
| T10257 |
9145-9361 |
sentence |
denotes |
In kidneys taken from mutant animals, however, AQP2 was distributed randomly throughout the cell in the basal state (Figure 4A, third row), while AQP3 (green) appropriately localized to the basolateral surface [23]. |
| T10258 |
9146-9148 |
IN |
denotes |
In |
| T10259 |
9202-9213 |
VBN |
denotes |
distributed |
| T10260 |
9149-9156 |
NNS |
denotes |
kidneys |
| T10261 |
9157-9162 |
VBN |
denotes |
taken |
| T10262 |
9163-9167 |
IN |
denotes |
from |
| T10263 |
9168-9174 |
NN |
denotes |
mutant |
| T10264 |
9175-9182 |
NNS |
denotes |
animals |
| T10265 |
9182-9184 |
, |
denotes |
, |
| T10266 |
9184-9191 |
RB |
denotes |
however |
| T10267 |
9191-9193 |
, |
denotes |
, |
| T10268 |
9193-9197 |
NN |
denotes |
AQP2 |
| T10269 |
9198-9201 |
VBD |
denotes |
was |
| T10270 |
9214-9222 |
RB |
denotes |
randomly |
| T10271 |
9223-9233 |
IN |
denotes |
throughout |
| T10272 |
9234-9237 |
DT |
denotes |
the |
| T10273 |
9238-9242 |
NN |
denotes |
cell |
| T10274 |
9243-9245 |
IN |
denotes |
in |
| T10275 |
9246-9249 |
DT |
denotes |
the |
| T10276 |
9256-9261 |
NN |
denotes |
state |
| T10277 |
9250-9255 |
JJ |
denotes |
basal |
| T10278 |
9262-9263 |
-LRB- |
denotes |
( |
| T10279 |
9280-9283 |
NN |
denotes |
row |
| T10280 |
9263-9269 |
NN |
denotes |
Figure |
| T10281 |
9270-9272 |
CD |
denotes |
4A |
| T10282 |
9272-9274 |
, |
denotes |
, |
| T10283 |
9274-9279 |
JJ |
denotes |
third |
| T10284 |
9283-9284 |
-RRB- |
denotes |
) |
| T10285 |
9284-9286 |
, |
denotes |
, |
| T10286 |
9286-9291 |
IN |
denotes |
while |
| T10287 |
9319-9328 |
VBD |
denotes |
localized |
| T10288 |
9292-9296 |
NN |
denotes |
AQP3 |
| T10289 |
9297-9298 |
-LRB- |
denotes |
( |
| T10290 |
9298-9303 |
NN |
denotes |
green |
| T10291 |
9303-9304 |
-RRB- |
denotes |
) |
| T10292 |
9305-9318 |
RB |
denotes |
appropriately |
| T10293 |
9329-9331 |
IN |
denotes |
to |
| T10294 |
9332-9335 |
DT |
denotes |
the |
| T10295 |
9348-9355 |
NN |
denotes |
surface |
| T10296 |
9336-9347 |
JJ |
denotes |
basolateral |
| T10297 |
9356-9357 |
-LRB- |
denotes |
[ |
| T10298 |
9357-9359 |
CD |
denotes |
23 |
| T10299 |
9359-9360 |
-RRB- |
denotes |
] |
| T10300 |
9360-9361 |
. |
denotes |
. |
| T10301 |
9361-9476 |
sentence |
denotes |
Furthermore, upon dDAVP stimulation, AQP2-F204V failed to translocate to the cell surface (Figure 4A, bottom row). |
| T10302 |
9362-9373 |
RB |
denotes |
Furthermore |
| T10303 |
9410-9416 |
VBD |
denotes |
failed |
| T10304 |
9373-9375 |
, |
denotes |
, |
| T10305 |
9375-9379 |
IN |
denotes |
upon |
| T10306 |
9380-9385 |
NN |
denotes |
dDAVP |
| T10307 |
9386-9397 |
NN |
denotes |
stimulation |
| T10308 |
9397-9399 |
, |
denotes |
, |
| T10309 |
9399-9403 |
NN |
denotes |
AQP2 |
| T10310 |
9404-9409 |
NN |
denotes |
F204V |
| T10311 |
9403-9404 |
HYPH |
denotes |
- |
| T10312 |
9417-9419 |
TO |
denotes |
to |
| T10313 |
9420-9431 |
VB |
denotes |
translocate |
| T10314 |
9432-9434 |
IN |
denotes |
to |
| T10315 |
9435-9438 |
DT |
denotes |
the |
| T10316 |
9444-9451 |
NN |
denotes |
surface |
| T10317 |
9439-9443 |
NN |
denotes |
cell |
| T10318 |
9452-9453 |
-LRB- |
denotes |
( |
| T10319 |
9471-9474 |
NN |
denotes |
row |
| T10320 |
9453-9459 |
NN |
denotes |
Figure |
| T10321 |
9460-9462 |
CD |
denotes |
4A |
| T10322 |
9462-9464 |
, |
denotes |
, |
| T10323 |
9464-9470 |
JJ |
denotes |
bottom |
| T10324 |
9474-9475 |
-RRB- |
denotes |
) |
| T10325 |
9475-9476 |
. |
denotes |
. |
| T10326 |
9476-9662 |
sentence |
denotes |
To confirm these findings, the staining was repeated in kidneys taken from two further mice for each class, wild-type or mutant, with or without dDAVP treatment, with identical results. |
| T10327 |
9477-9479 |
TO |
denotes |
To |
| T10328 |
9480-9487 |
VB |
denotes |
confirm |
| T10329 |
9521-9529 |
VBN |
denotes |
repeated |
| T10330 |
9488-9493 |
DT |
denotes |
these |
| T10331 |
9494-9502 |
NNS |
denotes |
findings |
| T10332 |
9502-9504 |
, |
denotes |
, |
| T10333 |
9504-9507 |
DT |
denotes |
the |
| T10334 |
9508-9516 |
NN |
denotes |
staining |
| T10335 |
9517-9520 |
VBD |
denotes |
was |
| T10336 |
9530-9532 |
IN |
denotes |
in |
| T10337 |
9533-9540 |
NNS |
denotes |
kidneys |
| T10338 |
9541-9546 |
VBN |
denotes |
taken |
| T10339 |
9547-9551 |
IN |
denotes |
from |
| T10340 |
9552-9555 |
CD |
denotes |
two |
| T10341 |
9564-9568 |
NNS |
denotes |
mice |
| T10342 |
9556-9563 |
JJ |
denotes |
further |
| T10343 |
9569-9572 |
IN |
denotes |
for |
| T10344 |
9573-9577 |
DT |
denotes |
each |
| T10345 |
9578-9583 |
NN |
denotes |
class |
| T10346 |
9583-9585 |
, |
denotes |
, |
| T10347 |
9585-9589 |
JJ |
denotes |
wild |
| T10348 |
9590-9594 |
NN |
denotes |
type |
| T10349 |
9589-9590 |
HYPH |
denotes |
- |
| T10350 |
9595-9597 |
CC |
denotes |
or |
| T10351 |
9598-9604 |
NN |
denotes |
mutant |
| T10352 |
9604-9606 |
, |
denotes |
, |
| T10353 |
9606-9610 |
IN |
denotes |
with |
| T10354 |
9611-9613 |
CC |
denotes |
or |
| T10355 |
9614-9621 |
IN |
denotes |
without |
| T10356 |
9622-9627 |
NN |
denotes |
dDAVP |
| T10357 |
9628-9637 |
NN |
denotes |
treatment |
| T10358 |
9637-9639 |
, |
denotes |
, |
| T10359 |
9639-9643 |
IN |
denotes |
with |
| T10360 |
9644-9653 |
JJ |
denotes |
identical |
| T10361 |
9654-9661 |
NNS |
denotes |
results |
| T10362 |
9661-9662 |
. |
denotes |
. |
| T10363 |
9662-11442 |
sentence |
denotes |
Figure 4 AQP2 Subcellular Localization and Translocation in Mouse Kidney Collecting Ducts and MDCK Cell Lines
(A) Immunohistochemistry on collecting ducts in kidney sections from an AQP2-F204V mutant (Mut) mouse and an age-sex matched wild-type (WT) littermate. Mice were injected intraperitoneally with PBS (NT) or dDAVP before sacrificing and fixation of the kidneys. Kidneys sections were immunostained for AQP2 (red) and the basolateral marker AQP3 (green). The images were merged and an area of the cytoplasm was magnified (zoom). Note that mutant AQP2 is not properly localized to the subapical compartment, nor does it respond to dDAVP.
(B) MDCK cell lines, stably transfected with constructs encoding mouse WT or AQP2-F204V, were treated with and without 150 μM forskolin for 90 min, after which cells were fixed, permeabilized, and subjected to immunocytochemistry. AQP2 is shown in green, and the basolateral marker Na+/K+-ATPase is shown in red, alongside the nuclear stain DAPI. The z-profile images were reconstructed from multiple z-sections, along the dotted line. Mutant AQP2 fails to localize to the cell surface upon forskolin stimulation. Rather, the perinuclear staining is consistent with an ER localization of mutant AQP2.
(C) The MDCK cell line expressing AQP2-F204V was grown on fibronectin-coated coverslips until tight junctions formed, at which point the cells were treated with 150 μM forskolin for 90 min. Cells were fixed, permeabilized, and sequentially immunoblotted for AQP2 (green) and calnexin (red), an ER marker. The merged image shows that AQP2-F204V colocalizes with the endoplasmic reticulum marker. Scale bar refers to 10 μm. To investigate the mechanism of defective translocation of AQP2-F204V, we turned to transfection of MDCK cells. |
| T10364 |
11331-11333 |
TO |
denotes |
To |
| T10365 |
11334-11345 |
VB |
denotes |
investigate |
| T10366 |
11405-11411 |
VBD |
denotes |
turned |
| T10367 |
11346-11349 |
DT |
denotes |
the |
| T10368 |
11350-11359 |
NN |
denotes |
mechanism |
| T10369 |
11360-11362 |
IN |
denotes |
of |
| T10370 |
11363-11372 |
JJ |
denotes |
defective |
| T10371 |
11373-11386 |
NN |
denotes |
translocation |
| T10372 |
11387-11389 |
IN |
denotes |
of |
| T10373 |
11390-11394 |
NN |
denotes |
AQP2 |
| T10374 |
11395-11400 |
NN |
denotes |
F204V |
| T10375 |
11394-11395 |
HYPH |
denotes |
- |
| T10376 |
11400-11402 |
, |
denotes |
, |
| T10377 |
11402-11404 |
PRP |
denotes |
we |
| T10378 |
11412-11414 |
IN |
denotes |
to |
| T10379 |
11415-11427 |
NN |
denotes |
transfection |
| T10380 |
11428-11430 |
IN |
denotes |
of |
| T10381 |
11431-11435 |
NN |
denotes |
MDCK |
| T10382 |
11436-11441 |
NNS |
denotes |
cells |
| T10383 |
11441-11442 |
. |
denotes |
. |
| T10384 |
11442-11525 |
sentence |
denotes |
Stable cell lines expressing mouse wild-type AQP2 and AQP2-F204V were established. |
| T10385 |
11443-11449 |
JJ |
denotes |
Stable |
| T10386 |
11455-11460 |
NNS |
denotes |
lines |
| T10387 |
11450-11454 |
NN |
denotes |
cell |
| T10388 |
11513-11524 |
VBN |
denotes |
established |
| T10389 |
11461-11471 |
VBG |
denotes |
expressing |
| T10390 |
11472-11477 |
NN |
denotes |
mouse |
| T10391 |
11488-11492 |
NN |
denotes |
AQP2 |
| T10392 |
11478-11482 |
JJ |
denotes |
wild |
| T10393 |
11483-11487 |
NN |
denotes |
type |
| T10394 |
11482-11483 |
HYPH |
denotes |
- |
| T10395 |
11493-11496 |
CC |
denotes |
and |
| T10396 |
11497-11501 |
NN |
denotes |
AQP2 |
| T10397 |
11502-11507 |
NN |
denotes |
F204V |
| T10398 |
11501-11502 |
HYPH |
denotes |
- |
| T10399 |
11508-11512 |
VBD |
denotes |
were |
| T10400 |
11524-11525 |
. |
denotes |
. |
| T10401 |
11525-11681 |
sentence |
denotes |
Immunoblots of protein extracts from stable cell lines showed that MDCK cells recapitulate the glycosylation defect seen in mutant mice (unpublished data). |
| T10402 |
11526-11537 |
NNS |
denotes |
Immunoblots |
| T10403 |
11581-11587 |
VBD |
denotes |
showed |
| T10404 |
11538-11540 |
IN |
denotes |
of |
| T10405 |
11541-11548 |
NN |
denotes |
protein |
| T10406 |
11549-11557 |
NNS |
denotes |
extracts |
| T10407 |
11558-11562 |
IN |
denotes |
from |
| T10408 |
11563-11569 |
JJ |
denotes |
stable |
| T10409 |
11575-11580 |
NNS |
denotes |
lines |
| T10410 |
11570-11574 |
NN |
denotes |
cell |
| T10411 |
11588-11592 |
IN |
denotes |
that |
| T10412 |
11604-11616 |
VBP |
denotes |
recapitulate |
| T10413 |
11593-11597 |
NN |
denotes |
MDCK |
| T10414 |
11598-11603 |
NNS |
denotes |
cells |
| T10415 |
11617-11620 |
DT |
denotes |
the |
| T10416 |
11635-11641 |
NN |
denotes |
defect |
| T10417 |
11621-11634 |
NN |
denotes |
glycosylation |
| T10418 |
11642-11646 |
VBN |
denotes |
seen |
| T10419 |
11647-11649 |
IN |
denotes |
in |
| T10420 |
11650-11656 |
NN |
denotes |
mutant |
| T10421 |
11657-11661 |
NNS |
denotes |
mice |
| T10422 |
11662-11663 |
-LRB- |
denotes |
( |
| T10423 |
11675-11679 |
NNS |
denotes |
data |
| T10424 |
11663-11674 |
JJ |
denotes |
unpublished |
| T10425 |
11679-11680 |
-RRB- |
denotes |
) |
| T10426 |
11680-11681 |
. |
denotes |
. |
| T10427 |
11681-11747 |
sentence |
denotes |
The wild-type protein was again present in three different forms. |
| T10428 |
11682-11685 |
DT |
denotes |
The |
| T10429 |
11696-11703 |
NN |
denotes |
protein |
| T10430 |
11686-11690 |
JJ |
denotes |
wild |
| T10431 |
11691-11695 |
NN |
denotes |
type |
| T10432 |
11690-11691 |
HYPH |
denotes |
- |
| T10433 |
11704-11707 |
VBD |
denotes |
was |
| T10434 |
11708-11713 |
RB |
denotes |
again |
| T10435 |
11714-11721 |
JJ |
denotes |
present |
| T10436 |
11722-11724 |
IN |
denotes |
in |
| T10437 |
11725-11730 |
CD |
denotes |
three |
| T10438 |
11741-11746 |
NNS |
denotes |
forms |
| T10439 |
11731-11740 |
JJ |
denotes |
different |
| T10440 |
11746-11747 |
. |
denotes |
. |
| T10441 |
11747-11857 |
sentence |
denotes |
Cells expressing AQP2-F204V lacked the 35–45 kDa form and were enriched in the core-glycosylated 31 kDa form. |
| T10442 |
11748-11753 |
NNS |
denotes |
Cells |
| T10443 |
11776-11782 |
VBD |
denotes |
lacked |
| T10444 |
11754-11764 |
VBG |
denotes |
expressing |
| T10445 |
11765-11769 |
NN |
denotes |
AQP2 |
| T10446 |
11770-11775 |
NN |
denotes |
F204V |
| T10447 |
11769-11770 |
HYPH |
denotes |
- |
| T10448 |
11783-11786 |
DT |
denotes |
the |
| T10449 |
11797-11801 |
NN |
denotes |
form |
| T10450 |
11787-11789 |
CD |
denotes |
35 |
| T10451 |
11790-11792 |
CD |
denotes |
45 |
| T10452 |
11789-11790 |
SYM |
denotes |
– |
| T10453 |
11793-11796 |
NN |
denotes |
kDa |
| T10454 |
11802-11805 |
CC |
denotes |
and |
| T10455 |
11806-11810 |
VBD |
denotes |
were |
| T10456 |
11811-11819 |
VBN |
denotes |
enriched |
| T10457 |
11820-11822 |
IN |
denotes |
in |
| T10458 |
11823-11826 |
DT |
denotes |
the |
| T10459 |
11852-11856 |
NN |
denotes |
form |
| T10460 |
11827-11831 |
NN |
denotes |
core |
| T10461 |
11832-11844 |
VBN |
denotes |
glycosylated |
| T10462 |
11831-11832 |
HYPH |
denotes |
- |
| T10463 |
11845-11847 |
CD |
denotes |
31 |
| T10464 |
11848-11851 |
NN |
denotes |
kDa |
| T10465 |
11856-11857 |
. |
denotes |
. |
| T10466 |
11857-12095 |
sentence |
denotes |
In transfected, unstimulated MDCK cells, wild-type AQP2 (stained green) appeared in a punctate pattern distributed throughout the subapical region (Figure 4B, left column photomicrographs), consistent with vesicular compartmentalization. |
| T10467 |
11858-11860 |
IN |
denotes |
In |
| T10468 |
11930-11938 |
VBD |
denotes |
appeared |
| T10469 |
11861-11872 |
VBN |
denotes |
transfected |
| T10470 |
11892-11897 |
NNS |
denotes |
cells |
| T10471 |
11872-11874 |
, |
denotes |
, |
| T10472 |
11874-11886 |
JJ |
denotes |
unstimulated |
| T10473 |
11887-11891 |
NN |
denotes |
MDCK |
| T10474 |
11897-11899 |
, |
denotes |
, |
| T10475 |
11899-11903 |
JJ |
denotes |
wild |
| T10476 |
11904-11908 |
NN |
denotes |
type |
| T10477 |
11903-11904 |
HYPH |
denotes |
- |
| T10478 |
11909-11913 |
NN |
denotes |
AQP2 |
| T10479 |
11914-11915 |
-LRB- |
denotes |
( |
| T10480 |
11915-11922 |
VBN |
denotes |
stained |
| T10481 |
11923-11928 |
JJ |
denotes |
green |
| T10482 |
11928-11929 |
-RRB- |
denotes |
) |
| T10483 |
11939-11941 |
IN |
denotes |
in |
| T10484 |
11942-11943 |
DT |
denotes |
a |
| T10485 |
11953-11960 |
NN |
denotes |
pattern |
| T10486 |
11944-11952 |
NN |
denotes |
punctate |
| T10487 |
11961-11972 |
VBN |
denotes |
distributed |
| T10488 |
11973-11983 |
IN |
denotes |
throughout |
| T10489 |
11984-11987 |
DT |
denotes |
the |
| T10490 |
11998-12004 |
NN |
denotes |
region |
| T10491 |
11988-11997 |
JJ |
denotes |
subapical |
| T10492 |
12005-12006 |
-LRB- |
denotes |
( |
| T10493 |
12029-12045 |
NNS |
denotes |
photomicrographs |
| T10494 |
12006-12012 |
NN |
denotes |
Figure |
| T10495 |
12013-12015 |
CD |
denotes |
4B |
| T10496 |
12015-12017 |
, |
denotes |
, |
| T10497 |
12017-12021 |
JJ |
denotes |
left |
| T10498 |
12022-12028 |
NN |
denotes |
column |
| T10499 |
12045-12046 |
-RRB- |
denotes |
) |
| T10500 |
12046-12048 |
, |
denotes |
, |
| T10501 |
12048-12058 |
JJ |
denotes |
consistent |
| T10502 |
12059-12063 |
IN |
denotes |
with |
| T10503 |
12064-12073 |
JJ |
denotes |
vesicular |
| T10504 |
12074-12094 |
NN |
denotes |
compartmentalization |
| T10505 |
12094-12095 |
. |
denotes |
. |
| T10506 |
12095-12200 |
sentence |
denotes |
AQP2-F204V, on the other hand, appeared in a punctate but perinuclear pattern (Figure 4B, third column). |
| T10507 |
12096-12100 |
NN |
denotes |
AQP2 |
| T10508 |
12101-12106 |
NN |
denotes |
F204V |
| T10509 |
12100-12101 |
HYPH |
denotes |
- |
| T10510 |
12127-12135 |
VBD |
denotes |
appeared |
| T10511 |
12106-12108 |
, |
denotes |
, |
| T10512 |
12108-12110 |
IN |
denotes |
on |
| T10513 |
12111-12114 |
DT |
denotes |
the |
| T10514 |
12121-12125 |
NN |
denotes |
hand |
| T10515 |
12115-12120 |
JJ |
denotes |
other |
| T10516 |
12125-12127 |
, |
denotes |
, |
| T10517 |
12136-12138 |
IN |
denotes |
in |
| T10518 |
12139-12140 |
DT |
denotes |
a |
| T10519 |
12166-12173 |
NN |
denotes |
pattern |
| T10520 |
12141-12149 |
NN |
denotes |
punctate |
| T10521 |
12154-12165 |
JJ |
denotes |
perinuclear |
| T10522 |
12150-12153 |
CC |
denotes |
but |
| T10523 |
12174-12175 |
-LRB- |
denotes |
( |
| T10524 |
12192-12198 |
NN |
denotes |
column |
| T10525 |
12175-12181 |
NN |
denotes |
Figure |
| T10526 |
12182-12184 |
CD |
denotes |
4B |
| T10527 |
12184-12186 |
, |
denotes |
, |
| T10528 |
12186-12191 |
JJ |
denotes |
third |
| T10529 |
12198-12199 |
-RRB- |
denotes |
) |
| T10530 |
12199-12200 |
. |
denotes |
. |
| T10531 |
12200-12378 |
sentence |
denotes |
Upon stimulation with forskolin, a cAMP-dependent protein kinase activator, wild-type AQP2 translocated to the apical surface of polarized MDCK cells (Figure 4B, second column). |
| T10532 |
12201-12205 |
IN |
denotes |
Upon |
| T10533 |
12292-12304 |
VBD |
denotes |
translocated |
| T10534 |
12206-12217 |
NN |
denotes |
stimulation |
| T10535 |
12218-12222 |
IN |
denotes |
with |
| T10536 |
12223-12232 |
NN |
denotes |
forskolin |
| T10537 |
12232-12234 |
, |
denotes |
, |
| T10538 |
12234-12235 |
DT |
denotes |
a |
| T10539 |
12266-12275 |
NN |
denotes |
activator |
| T10540 |
12236-12240 |
NN |
denotes |
cAMP |
| T10541 |
12241-12250 |
JJ |
denotes |
dependent |
| T10542 |
12240-12241 |
HYPH |
denotes |
- |
| T10543 |
12251-12258 |
NN |
denotes |
protein |
| T10544 |
12259-12265 |
NN |
denotes |
kinase |
| T10545 |
12275-12277 |
, |
denotes |
, |
| T10546 |
12277-12281 |
JJ |
denotes |
wild |
| T10547 |
12282-12286 |
NN |
denotes |
type |
| T10548 |
12281-12282 |
HYPH |
denotes |
- |
| T10549 |
12287-12291 |
NN |
denotes |
AQP2 |
| T10550 |
12305-12307 |
IN |
denotes |
to |
| T10551 |
12308-12311 |
DT |
denotes |
the |
| T10552 |
12319-12326 |
NN |
denotes |
surface |
| T10553 |
12312-12318 |
JJ |
denotes |
apical |
| T10554 |
12327-12329 |
IN |
denotes |
of |
| T10555 |
12330-12339 |
VBN |
denotes |
polarized |
| T10556 |
12345-12350 |
NNS |
denotes |
cells |
| T10557 |
12340-12344 |
NN |
denotes |
MDCK |
| T10558 |
12351-12352 |
-LRB- |
denotes |
( |
| T10559 |
12370-12376 |
NN |
denotes |
column |
| T10560 |
12352-12358 |
NN |
denotes |
Figure |
| T10561 |
12359-12361 |
CD |
denotes |
4B |
| T10562 |
12361-12363 |
, |
denotes |
, |
| T10563 |
12363-12369 |
JJ |
denotes |
second |
| T10564 |
12376-12377 |
-RRB- |
denotes |
) |
| T10565 |
12377-12378 |
. |
denotes |
. |
| T10566 |
12378-12551 |
sentence |
denotes |
Along the z-axis, the perinuclear distribution of AQP2-F204V was clearly seen, and this distribution is not altered by forskolin (Figure 4B, bottom row, two right columns). |
| T10567 |
12379-12384 |
IN |
denotes |
Along |
| T10568 |
12452-12456 |
VBN |
denotes |
seen |
| T10569 |
12385-12388 |
DT |
denotes |
the |
| T10570 |
12391-12395 |
NN |
denotes |
axis |
| T10571 |
12389-12390 |
NN |
denotes |
z |
| T10572 |
12390-12391 |
HYPH |
denotes |
- |
| T10573 |
12395-12397 |
, |
denotes |
, |
| T10574 |
12397-12400 |
DT |
denotes |
the |
| T10575 |
12413-12425 |
NN |
denotes |
distribution |
| T10576 |
12401-12412 |
JJ |
denotes |
perinuclear |
| T10577 |
12426-12428 |
IN |
denotes |
of |
| T10578 |
12429-12433 |
NN |
denotes |
AQP2 |
| T10579 |
12434-12439 |
NN |
denotes |
F204V |
| T10580 |
12433-12434 |
HYPH |
denotes |
- |
| T10581 |
12440-12443 |
VBD |
denotes |
was |
| T10582 |
12444-12451 |
RB |
denotes |
clearly |
| T10583 |
12456-12458 |
, |
denotes |
, |
| T10584 |
12458-12461 |
CC |
denotes |
and |
| T10585 |
12462-12466 |
DT |
denotes |
this |
| T10586 |
12467-12479 |
NN |
denotes |
distribution |
| T10587 |
12487-12494 |
VBN |
denotes |
altered |
| T10588 |
12480-12482 |
VBZ |
denotes |
is |
| T10589 |
12483-12486 |
RB |
denotes |
not |
| T10590 |
12495-12497 |
IN |
denotes |
by |
| T10591 |
12498-12507 |
NN |
denotes |
forskolin |
| T10592 |
12508-12509 |
-LRB- |
denotes |
( |
| T10593 |
12542-12549 |
NNS |
denotes |
columns |
| T10594 |
12509-12515 |
NN |
denotes |
Figure |
| T10595 |
12516-12518 |
CD |
denotes |
4B |
| T10596 |
12518-12520 |
, |
denotes |
, |
| T10597 |
12520-12526 |
JJ |
denotes |
bottom |
| T10598 |
12527-12530 |
NN |
denotes |
row |
| T10599 |
12530-12532 |
, |
denotes |
, |
| T10600 |
12532-12535 |
CD |
denotes |
two |
| T10601 |
12536-12541 |
JJ |
denotes |
right |
| T10602 |
12549-12550 |
-RRB- |
denotes |
) |
| T10603 |
12550-12551 |
. |
denotes |
. |
| T10604 |
12551-12641 |
sentence |
denotes |
The perinuclear distribution of AQP2-F204V is consistent with an ER compartmentalization. |
| T10605 |
12552-12555 |
DT |
denotes |
The |
| T10606 |
12568-12580 |
NN |
denotes |
distribution |
| T10607 |
12556-12567 |
JJ |
denotes |
perinuclear |
| T10608 |
12595-12597 |
VBZ |
denotes |
is |
| T10609 |
12581-12583 |
IN |
denotes |
of |
| T10610 |
12584-12588 |
NN |
denotes |
AQP2 |
| T10611 |
12589-12594 |
NN |
denotes |
F204V |
| T10612 |
12588-12589 |
HYPH |
denotes |
- |
| T10613 |
12598-12608 |
JJ |
denotes |
consistent |
| T10614 |
12609-12613 |
IN |
denotes |
with |
| T10615 |
12614-12616 |
DT |
denotes |
an |
| T10616 |
12620-12640 |
NN |
denotes |
compartmentalization |
| T10617 |
12617-12619 |
NN |
denotes |
ER |
| T10618 |
12640-12641 |
. |
denotes |
. |
| T10619 |
12641-12798 |
sentence |
denotes |
To test the idea that AQP2-F204V localizes to the ER, we co-stained cells transfected with Aqp2F204V (cDNA) for AQP2 and an ER marker, calnexin (Figure 4C). |
| T10620 |
12642-12644 |
TO |
denotes |
To |
| T10621 |
12645-12649 |
VB |
denotes |
test |
| T10622 |
12699-12709 |
VBD |
denotes |
co-stained |
| T10623 |
12650-12653 |
DT |
denotes |
the |
| T10624 |
12654-12658 |
NN |
denotes |
idea |
| T10625 |
12659-12663 |
IN |
denotes |
that |
| T10626 |
12675-12684 |
VBZ |
denotes |
localizes |
| T10627 |
12664-12668 |
NN |
denotes |
AQP2 |
| T10628 |
12669-12674 |
NN |
denotes |
F204V |
| T10629 |
12668-12669 |
HYPH |
denotes |
- |
| T10630 |
12685-12687 |
IN |
denotes |
to |
| T10631 |
12688-12691 |
DT |
denotes |
the |
| T10632 |
12692-12694 |
NN |
denotes |
ER |
| T10633 |
12694-12696 |
, |
denotes |
, |
| T10634 |
12696-12698 |
PRP |
denotes |
we |
| T10635 |
12710-12715 |
NNS |
denotes |
cells |
| T10636 |
12716-12727 |
VBN |
denotes |
transfected |
| T10637 |
12728-12732 |
IN |
denotes |
with |
| T10638 |
12733-12742 |
NN |
denotes |
Aqp2F204V |
| T10639 |
12743-12744 |
-LRB- |
denotes |
( |
| T10640 |
12744-12748 |
NN |
denotes |
cDNA |
| T10641 |
12748-12749 |
-RRB- |
denotes |
) |
| T10642 |
12750-12753 |
IN |
denotes |
for |
| T10643 |
12754-12758 |
NN |
denotes |
AQP2 |
| T10644 |
12759-12762 |
CC |
denotes |
and |
| T10645 |
12763-12765 |
DT |
denotes |
an |
| T10646 |
12769-12775 |
NN |
denotes |
marker |
| T10647 |
12766-12768 |
NN |
denotes |
ER |
| T10648 |
12775-12777 |
, |
denotes |
, |
| T10649 |
12777-12785 |
NN |
denotes |
calnexin |
| T10650 |
12786-12787 |
-LRB- |
denotes |
( |
| T10651 |
12787-12793 |
NN |
denotes |
Figure |
| T10652 |
12794-12796 |
CD |
denotes |
4C |
| T10653 |
12796-12797 |
-RRB- |
denotes |
) |
| T10654 |
12797-12798 |
. |
denotes |
. |
| T10655 |
12798-12941 |
sentence |
denotes |
Colocalization of calnexin with AQP2 was investigated directly, and it was found that 80% of all AQP2-F204V protein colocalized with calnexin. |
| T10656 |
12799-12813 |
NN |
denotes |
Colocalization |
| T10657 |
12840-12852 |
VBN |
denotes |
investigated |
| T10658 |
12814-12816 |
IN |
denotes |
of |
| T10659 |
12817-12825 |
NN |
denotes |
calnexin |
| T10660 |
12826-12830 |
IN |
denotes |
with |
| T10661 |
12831-12835 |
NN |
denotes |
AQP2 |
| T10662 |
12836-12839 |
VBD |
denotes |
was |
| T10663 |
12853-12861 |
RB |
denotes |
directly |
| T10664 |
12861-12863 |
, |
denotes |
, |
| T10665 |
12863-12866 |
CC |
denotes |
and |
| T10666 |
12867-12869 |
PRP |
denotes |
it |
| T10667 |
12874-12879 |
VBN |
denotes |
found |
| T10668 |
12870-12873 |
VBD |
denotes |
was |
| T10669 |
12880-12884 |
IN |
denotes |
that |
| T10670 |
12915-12926 |
VBD |
denotes |
colocalized |
| T10671 |
12885-12887 |
CD |
denotes |
80 |
| T10672 |
12887-12888 |
NN |
denotes |
% |
| T10673 |
12889-12891 |
IN |
denotes |
of |
| T10674 |
12892-12895 |
DT |
denotes |
all |
| T10675 |
12907-12914 |
NN |
denotes |
protein |
| T10676 |
12896-12900 |
NN |
denotes |
AQP2 |
| T10677 |
12901-12906 |
NN |
denotes |
F204V |
| T10678 |
12900-12901 |
HYPH |
denotes |
- |
| T10679 |
12927-12931 |
IN |
denotes |
with |
| T10680 |
12932-12940 |
NN |
denotes |
calnexin |
| T10681 |
12940-12941 |
. |
denotes |
. |
| T10682 |
12941-13067 |
sentence |
denotes |
The remaining 20% appeared at the periphery of the ER, representing AQP2-F204V that had potentially progressed beyond the ER. |
| T10683 |
12942-12945 |
DT |
denotes |
The |
| T10684 |
12958-12959 |
NN |
denotes |
% |
| T10685 |
12946-12955 |
VBG |
denotes |
remaining |
| T10686 |
12956-12958 |
CD |
denotes |
20 |
| T10687 |
12960-12968 |
VBD |
denotes |
appeared |
| T10688 |
12969-12971 |
IN |
denotes |
at |
| T10689 |
12972-12975 |
DT |
denotes |
the |
| T10690 |
12976-12985 |
NN |
denotes |
periphery |
| T10691 |
12986-12988 |
IN |
denotes |
of |
| T10692 |
12989-12992 |
DT |
denotes |
the |
| T10693 |
12993-12995 |
NN |
denotes |
ER |
| T10694 |
12995-12997 |
, |
denotes |
, |
| T10695 |
12997-13009 |
VBG |
denotes |
representing |
| T10696 |
13010-13014 |
NN |
denotes |
AQP2 |
| T10697 |
13015-13020 |
NN |
denotes |
F204V |
| T10698 |
13014-13015 |
HYPH |
denotes |
- |
| T10699 |
13021-13025 |
WDT |
denotes |
that |
| T10700 |
13042-13052 |
VBN |
denotes |
progressed |
| T10701 |
13026-13029 |
VBD |
denotes |
had |
| T10702 |
13030-13041 |
RB |
denotes |
potentially |
| T10703 |
13053-13059 |
IN |
denotes |
beyond |
| T10704 |
13060-13063 |
DT |
denotes |
the |
| T10705 |
13064-13066 |
NN |
denotes |
ER |
| T10706 |
13066-13067 |
. |
denotes |
. |
| T10707 |
13067-13204 |
sentence |
denotes |
This “ER escape” was consistent with the small proportion of mature, complex glycosylated, AQP2-F204V in mutant kidneys (see Figure 3A). |
| T10708 |
13068-13072 |
DT |
denotes |
This |
| T10709 |
13077-13083 |
NN |
denotes |
escape |
| T10710 |
13073-13074 |
`` |
denotes |
“ |
| T10711 |
13074-13076 |
NN |
denotes |
ER |
| T10712 |
13085-13088 |
VBD |
denotes |
was |
| T10713 |
13083-13084 |
'' |
denotes |
” |
| T10714 |
13089-13099 |
JJ |
denotes |
consistent |
| T10715 |
13100-13104 |
IN |
denotes |
with |
| T10716 |
13105-13108 |
DT |
denotes |
the |
| T10717 |
13115-13125 |
NN |
denotes |
proportion |
| T10718 |
13109-13114 |
JJ |
denotes |
small |
| T10719 |
13126-13128 |
IN |
denotes |
of |
| T10720 |
13129-13135 |
JJ |
denotes |
mature |
| T10721 |
13164-13169 |
NN |
denotes |
F204V |
| T10722 |
13135-13137 |
, |
denotes |
, |
| T10723 |
13137-13144 |
JJ |
denotes |
complex |
| T10724 |
13145-13157 |
VBN |
denotes |
glycosylated |
| T10725 |
13157-13159 |
, |
denotes |
, |
| T10726 |
13159-13163 |
NN |
denotes |
AQP2 |
| T10727 |
13163-13164 |
HYPH |
denotes |
- |
| T10728 |
13170-13172 |
IN |
denotes |
in |
| T10729 |
13173-13179 |
NN |
denotes |
mutant |
| T10730 |
13180-13187 |
NNS |
denotes |
kidneys |
| T10731 |
13188-13189 |
-LRB- |
denotes |
( |
| T10732 |
13189-13192 |
VB |
denotes |
see |
| T10733 |
13193-13199 |
NN |
denotes |
Figure |
| T10734 |
13200-13202 |
CD |
denotes |
3A |
| T10735 |
13202-13203 |
-RRB- |
denotes |
) |
| T10736 |
13203-13204 |
. |
denotes |
. |
| T10737 |
13204-13340 |
sentence |
denotes |
Animals heterozygous for the Aqp2F204V mutation were not affected in their urine production or urine osmolality (see Figure 1C and 1D). |
| T10738 |
13205-13212 |
NNS |
denotes |
Animals |
| T10739 |
13262-13270 |
VBN |
denotes |
affected |
| T10740 |
13213-13225 |
JJ |
denotes |
heterozygous |
| T10741 |
13226-13229 |
IN |
denotes |
for |
| T10742 |
13230-13233 |
DT |
denotes |
the |
| T10743 |
13244-13252 |
NN |
denotes |
mutation |
| T10744 |
13234-13243 |
NN |
denotes |
Aqp2F204V |
| T10745 |
13253-13257 |
VBD |
denotes |
were |
| T10746 |
13258-13261 |
RB |
denotes |
not |
| T10747 |
13271-13273 |
IN |
denotes |
in |
| T10748 |
13274-13279 |
PRP$ |
denotes |
their |
| T10749 |
13286-13296 |
NN |
denotes |
production |
| T10750 |
13280-13285 |
NN |
denotes |
urine |
| T10751 |
13297-13299 |
CC |
denotes |
or |
| T10752 |
13300-13305 |
NN |
denotes |
urine |
| T10753 |
13306-13316 |
NN |
denotes |
osmolality |
| T10754 |
13317-13318 |
-LRB- |
denotes |
( |
| T10755 |
13318-13321 |
VB |
denotes |
see |
| T10756 |
13322-13328 |
NN |
denotes |
Figure |
| T10757 |
13329-13331 |
CD |
denotes |
1C |
| T10758 |
13332-13335 |
CC |
denotes |
and |
| T10759 |
13336-13338 |
CD |
denotes |
1D |
| T10760 |
13338-13339 |
-RRB- |
denotes |
) |
| T10761 |
13339-13340 |
. |
denotes |
. |
| T10762 |
13340-13511 |
sentence |
denotes |
It has also been shown that a recessive NDI allele, AQP2-R187C, does not interact with wild-type protein in oocytes [24], nor does it homo-oligomerize in MDCK cells [22]. |
| T10763 |
13341-13343 |
PRP |
denotes |
It |
| T10764 |
13358-13363 |
VBN |
denotes |
shown |
| T10765 |
13344-13347 |
VBZ |
denotes |
has |
| T10766 |
13348-13352 |
RB |
denotes |
also |
| T10767 |
13353-13357 |
VBN |
denotes |
been |
| T10768 |
13364-13368 |
IN |
denotes |
that |
| T10769 |
13414-13422 |
VB |
denotes |
interact |
| T10770 |
13369-13370 |
DT |
denotes |
a |
| T10771 |
13385-13391 |
NN |
denotes |
allele |
| T10772 |
13371-13380 |
JJ |
denotes |
recessive |
| T10773 |
13381-13384 |
NN |
denotes |
NDI |
| T10774 |
13391-13393 |
, |
denotes |
, |
| T10775 |
13393-13397 |
NN |
denotes |
AQP2 |
| T10776 |
13398-13403 |
NN |
denotes |
R187C |
| T10777 |
13397-13398 |
HYPH |
denotes |
- |
| T10778 |
13403-13405 |
, |
denotes |
, |
| T10779 |
13405-13409 |
VBZ |
denotes |
does |
| T10780 |
13410-13413 |
RB |
denotes |
not |
| T10781 |
13423-13427 |
IN |
denotes |
with |
| T10782 |
13428-13432 |
JJ |
denotes |
wild |
| T10783 |
13433-13437 |
NN |
denotes |
type |
| T10784 |
13432-13433 |
HYPH |
denotes |
- |
| T10785 |
13438-13445 |
NN |
denotes |
protein |
| T10786 |
13446-13448 |
IN |
denotes |
in |
| T10787 |
13449-13456 |
NNS |
denotes |
oocytes |
| T10788 |
13457-13458 |
-LRB- |
denotes |
[ |
| T10789 |
13458-13460 |
CD |
denotes |
24 |
| T10790 |
13460-13461 |
-RRB- |
denotes |
] |
| T10791 |
13461-13463 |
, |
denotes |
, |
| T10792 |
13463-13466 |
CC |
denotes |
nor |
| T10793 |
13467-13471 |
VBZ |
denotes |
does |
| T10794 |
13475-13491 |
VB |
denotes |
homo-oligomerize |
| T10795 |
13472-13474 |
PRP |
denotes |
it |
| T10796 |
13492-13494 |
IN |
denotes |
in |
| T10797 |
13495-13499 |
NN |
denotes |
MDCK |
| T10798 |
13500-13505 |
NNS |
denotes |
cells |
| T10799 |
13506-13507 |
-LRB- |
denotes |
[ |
| T10800 |
13507-13509 |
CD |
denotes |
22 |
| T10801 |
13509-13510 |
-RRB- |
denotes |
] |
| T10802 |
13510-13511 |
. |
denotes |
. |
| T10803 |
13511-13619 |
sentence |
denotes |
Therefore, kidneys from heterozygous animals were examined for evidence of two populations of AQP2 protein. |
| T10804 |
13512-13521 |
RB |
denotes |
Therefore |
| T10805 |
13562-13570 |
VBN |
denotes |
examined |
| T10806 |
13521-13523 |
, |
denotes |
, |
| T10807 |
13523-13530 |
NNS |
denotes |
kidneys |
| T10808 |
13531-13535 |
IN |
denotes |
from |
| T10809 |
13536-13548 |
JJ |
denotes |
heterozygous |
| T10810 |
13549-13556 |
NNS |
denotes |
animals |
| T10811 |
13557-13561 |
VBD |
denotes |
were |
| T10812 |
13571-13574 |
IN |
denotes |
for |
| T10813 |
13575-13583 |
NN |
denotes |
evidence |
| T10814 |
13584-13586 |
IN |
denotes |
of |
| T10815 |
13587-13590 |
CD |
denotes |
two |
| T10816 |
13591-13602 |
NNS |
denotes |
populations |
| T10817 |
13603-13605 |
IN |
denotes |
of |
| T10818 |
13606-13610 |
NN |
denotes |
AQP2 |
| T10819 |
13611-13618 |
NN |
denotes |
protein |
| T10820 |
13618-13619 |
. |
denotes |
. |
| T10821 |
13619-13775 |
sentence |
denotes |
Surprisingly, immunohistochemical staining of kidney collecting ducts from Aqp2F204V/+ mice revealed a pattern remarkably similar to wild type (Figure 5A). |
| T10822 |
13620-13632 |
RB |
denotes |
Surprisingly |
| T10823 |
13712-13720 |
VBD |
denotes |
revealed |
| T10824 |
13632-13634 |
, |
denotes |
, |
| T10825 |
13634-13653 |
JJ |
denotes |
immunohistochemical |
| T10826 |
13654-13662 |
NN |
denotes |
staining |
| T10827 |
13663-13665 |
IN |
denotes |
of |
| T10828 |
13666-13672 |
NN |
denotes |
kidney |
| T10829 |
13684-13689 |
NNS |
denotes |
ducts |
| T10830 |
13673-13683 |
VBG |
denotes |
collecting |
| T10831 |
13690-13694 |
IN |
denotes |
from |
| T10832 |
13695-13704 |
NN |
denotes |
Aqp2F204V |
| T10833 |
13707-13711 |
NNS |
denotes |
mice |
| T10834 |
13704-13705 |
HYPH |
denotes |
/ |
| T10835 |
13705-13706 |
SYM |
denotes |
+ |
| T10836 |
13721-13722 |
DT |
denotes |
a |
| T10837 |
13723-13730 |
NN |
denotes |
pattern |
| T10838 |
13731-13741 |
RB |
denotes |
remarkably |
| T10839 |
13742-13749 |
JJ |
denotes |
similar |
| T10840 |
13750-13752 |
IN |
denotes |
to |
| T10841 |
13753-13757 |
JJ |
denotes |
wild |
| T10842 |
13758-13762 |
NN |
denotes |
type |
| T10843 |
13763-13764 |
-LRB- |
denotes |
( |
| T10844 |
13764-13770 |
NN |
denotes |
Figure |
| T10845 |
13771-13773 |
CD |
denotes |
5A |
| T10846 |
13773-13774 |
-RRB- |
denotes |
) |
| T10847 |
13774-13775 |
. |
denotes |
. |
| T10848 |
13775-13855 |
sentence |
denotes |
AQP2 translocated completely to the apical cell surface upon dDAVP stimulation. |
| T10849 |
13776-13780 |
NN |
denotes |
AQP2 |
| T10850 |
13781-13793 |
VBD |
denotes |
translocated |
| T10851 |
13794-13804 |
RB |
denotes |
completely |
| T10852 |
13805-13807 |
IN |
denotes |
to |
| T10853 |
13808-13811 |
DT |
denotes |
the |
| T10854 |
13824-13831 |
NN |
denotes |
surface |
| T10855 |
13812-13818 |
JJ |
denotes |
apical |
| T10856 |
13819-13823 |
NN |
denotes |
cell |
| T10857 |
13832-13836 |
IN |
denotes |
upon |
| T10858 |
13837-13842 |
NN |
denotes |
dDAVP |
| T10859 |
13843-13854 |
NN |
denotes |
stimulation |
| T10860 |
13854-13855 |
. |
denotes |
. |
| T10861 |
13855-14014 |
sentence |
denotes |
This wild-type staining pattern may simply reflect the fact that decreasing the amount of mutant protein by half makes it undetectable by immunocytochemistry. |
| T10862 |
13856-13860 |
DT |
denotes |
This |
| T10863 |
13880-13887 |
NN |
denotes |
pattern |
| T10864 |
13861-13865 |
JJ |
denotes |
wild |
| T10865 |
13866-13870 |
NN |
denotes |
type |
| T10866 |
13865-13866 |
HYPH |
denotes |
- |
| T10867 |
13871-13879 |
NN |
denotes |
staining |
| T10868 |
13899-13906 |
VB |
denotes |
reflect |
| T10869 |
13888-13891 |
MD |
denotes |
may |
| T10870 |
13892-13898 |
RB |
denotes |
simply |
| T10871 |
13907-13910 |
DT |
denotes |
the |
| T10872 |
13911-13915 |
NN |
denotes |
fact |
| T10873 |
13916-13920 |
IN |
denotes |
that |
| T10874 |
13969-13974 |
VBZ |
denotes |
makes |
| T10875 |
13921-13931 |
VBG |
denotes |
decreasing |
| T10876 |
13932-13935 |
DT |
denotes |
the |
| T10877 |
13936-13942 |
NN |
denotes |
amount |
| T10878 |
13943-13945 |
IN |
denotes |
of |
| T10879 |
13946-13952 |
NN |
denotes |
mutant |
| T10880 |
13953-13960 |
NN |
denotes |
protein |
| T10881 |
13961-13963 |
IN |
denotes |
by |
| T10882 |
13964-13968 |
NN |
denotes |
half |
| T10883 |
13975-13977 |
PRP |
denotes |
it |
| T10884 |
13978-13990 |
JJ |
denotes |
undetectable |
| T10885 |
13991-13993 |
IN |
denotes |
by |
| T10886 |
13994-14013 |
NN |
denotes |
immunocytochemistry |
| T10887 |
14013-14014 |
. |
denotes |
. |
| T10888 |
14014-14113 |
sentence |
denotes |
Alternatively, the presence of wild-type protein may alter the localization of the mutant protein. |
| T10889 |
14015-14028 |
RB |
denotes |
Alternatively |
| T10890 |
14068-14073 |
VB |
denotes |
alter |
| T10891 |
14028-14030 |
, |
denotes |
, |
| T10892 |
14030-14033 |
DT |
denotes |
the |
| T10893 |
14034-14042 |
NN |
denotes |
presence |
| T10894 |
14043-14045 |
IN |
denotes |
of |
| T10895 |
14046-14050 |
JJ |
denotes |
wild |
| T10896 |
14051-14055 |
NN |
denotes |
type |
| T10897 |
14050-14051 |
HYPH |
denotes |
- |
| T10898 |
14056-14063 |
NN |
denotes |
protein |
| T10899 |
14064-14067 |
MD |
denotes |
may |
| T10900 |
14074-14077 |
DT |
denotes |
the |
| T10901 |
14078-14090 |
NN |
denotes |
localization |
| T10902 |
14091-14093 |
IN |
denotes |
of |
| T10903 |
14094-14097 |
DT |
denotes |
the |
| T10904 |
14105-14112 |
NN |
denotes |
protein |
| T10905 |
14098-14104 |
NN |
denotes |
mutant |
| T10906 |
14112-14113 |
. |
denotes |
. |
| T10907 |
14113-14283 |
sentence |
denotes |
Indeed, Hendriks et al. proposed a “piggy-back” mechanism to explain the transport of nonglycosylated subunits of AQP2 to the cell surface by glycosylated subunits [22]. |
| T10908 |
14114-14120 |
RB |
denotes |
Indeed |
| T10909 |
14138-14146 |
VBD |
denotes |
proposed |
| T10910 |
14120-14122 |
, |
denotes |
, |
| T10911 |
14122-14130 |
NNP |
denotes |
Hendriks |
| T10912 |
14131-14133 |
FW |
denotes |
et |
| T10913 |
14134-14137 |
FW |
denotes |
al. |
| T10914 |
14147-14148 |
DT |
denotes |
a |
| T10915 |
14162-14171 |
NN |
denotes |
mechanism |
| T10916 |
14149-14150 |
`` |
denotes |
“ |
| T10917 |
14150-14155 |
NN |
denotes |
piggy |
| T10918 |
14156-14160 |
NN |
denotes |
back |
| T10919 |
14155-14156 |
HYPH |
denotes |
- |
| T10920 |
14160-14161 |
'' |
denotes |
” |
| T10921 |
14172-14174 |
TO |
denotes |
to |
| T10922 |
14175-14182 |
VB |
denotes |
explain |
| T10923 |
14183-14186 |
DT |
denotes |
the |
| T10924 |
14187-14196 |
NN |
denotes |
transport |
| T10925 |
14197-14199 |
IN |
denotes |
of |
| T10926 |
14200-14215 |
JJ |
denotes |
nonglycosylated |
| T10927 |
14216-14224 |
NNS |
denotes |
subunits |
| T10928 |
14225-14227 |
IN |
denotes |
of |
| T10929 |
14228-14232 |
NN |
denotes |
AQP2 |
| T10930 |
14233-14235 |
IN |
denotes |
to |
| T10931 |
14236-14239 |
DT |
denotes |
the |
| T10932 |
14245-14252 |
NN |
denotes |
surface |
| T10933 |
14240-14244 |
NN |
denotes |
cell |
| T10934 |
14253-14255 |
IN |
denotes |
by |
| T10935 |
14256-14268 |
VBN |
denotes |
glycosylated |
| T10936 |
14269-14277 |
NNS |
denotes |
subunits |
| T10937 |
14278-14279 |
-LRB- |
denotes |
[ |
| T10938 |
14279-14281 |
CD |
denotes |
22 |
| T10939 |
14281-14282 |
-RRB- |
denotes |
] |
| T10940 |
14282-14283 |
. |
denotes |
. |
| T10941 |
14283-14448 |
sentence |
denotes |
It has also been shown that wild-type AQP2 protein can rescue a translocation-defective mutant protein, AQP2-P262L, when the two are coexpressed in MDCK cells [25]. |
| T10942 |
14284-14286 |
PRP |
denotes |
It |
| T10943 |
14301-14306 |
VBN |
denotes |
shown |
| T10944 |
14287-14290 |
VBZ |
denotes |
has |
| T10945 |
14291-14295 |
RB |
denotes |
also |
| T10946 |
14296-14300 |
VBN |
denotes |
been |
| T10947 |
14307-14311 |
IN |
denotes |
that |
| T10948 |
14339-14345 |
VB |
denotes |
rescue |
| T10949 |
14312-14316 |
JJ |
denotes |
wild |
| T10950 |
14317-14321 |
NN |
denotes |
type |
| T10951 |
14316-14317 |
HYPH |
denotes |
- |
| T10952 |
14327-14334 |
NN |
denotes |
protein |
| T10953 |
14322-14326 |
NN |
denotes |
AQP2 |
| T10954 |
14335-14338 |
MD |
denotes |
can |
| T10955 |
14346-14347 |
DT |
denotes |
a |
| T10956 |
14379-14386 |
NN |
denotes |
protein |
| T10957 |
14348-14361 |
NN |
denotes |
translocation |
| T10958 |
14362-14371 |
JJ |
denotes |
defective |
| T10959 |
14361-14362 |
HYPH |
denotes |
- |
| T10960 |
14372-14378 |
NN |
denotes |
mutant |
| T10961 |
14386-14388 |
, |
denotes |
, |
| T10962 |
14388-14392 |
NN |
denotes |
AQP2 |
| T10963 |
14393-14398 |
NN |
denotes |
P262L |
| T10964 |
14392-14393 |
HYPH |
denotes |
- |
| T10965 |
14398-14400 |
, |
denotes |
, |
| T10966 |
14400-14404 |
WRB |
denotes |
when |
| T10967 |
14417-14428 |
VBN |
denotes |
coexpressed |
| T10968 |
14405-14408 |
DT |
denotes |
the |
| T10969 |
14409-14412 |
CD |
denotes |
two |
| T10970 |
14413-14416 |
VBP |
denotes |
are |
| T10971 |
14429-14431 |
IN |
denotes |
in |
| T10972 |
14432-14436 |
NN |
denotes |
MDCK |
| T10973 |
14437-14442 |
NNS |
denotes |
cells |
| T10974 |
14443-14444 |
-LRB- |
denotes |
[ |
| T10975 |
14444-14446 |
CD |
denotes |
25 |
| T10976 |
14446-14447 |
-RRB- |
denotes |
] |
| T10977 |
14447-14448 |
. |
denotes |
. |
| T10978 |
14448-16211 |
sentence |
denotes |
Figure 5 AQP2-F204V Rescue in Heterozygous Mouse Collecting Ducts and in Cotransfected MDCK Cells
(A) In heterozygous animals, AQP2 localizes and responds to dDAVP normally. Immunohistochemistry was carried out on kidney sections from an Aqp2F204V/+ mouse, after injection with dDAVP. Kidney sections were sequentially immunostained for AQP2 (red) and the basolateral marker AQP3 (green).
(B) Mutant and wild-type AQP2 physically interact. MDCK cells stably expressing wild-type AQP2 were transiently transfected with GFP tagged wild-type AQP2, AQP2-F204V, or GFP alone. Solubilized membranes were immunoprecipitated with a GFP antibody. Total membranes and immunoprecipitates (GFP-IP) were Western blotted using an antibody against AQP2 (arrow) or AQP2-GFP fusions (arrowhead).
(C) Wild-type AQP2 rescues the localization defect of mutant AQP2. GFP fusions of either wild-type AQP2 (WT-GFP, top photomicrographs) or F204V AQP2 (F204V-GFP, bottom photomicrographs) were expressed in polarized MDCK stable cell lines expressing vector alone (vector, left photomicrographs) or AQP2-WT (right photomicrographs). Cells were stimulated with forskolin, processed for immunocytochemistry, and used to generate z-sectional images.
(D) Mutant AQP2 is present at the cell surface in cells coexpressing wild-type AQP2 (AQP2-WT). GFP fused to AQP2-F204V was expressed in MDCK cells expressing wild-type AQP2 or vector alone. Cells were stimulated with forskolin, and cell surface biotinylated proteins were precipitated then analyzed for the presence of wild-type AQP2 (arrow) and AQP2-F204V (arrowhead) by Western blot. In the collecting ducts from Aqp2F204V/+ mice, the wild type may rescue the mutant protein as suggested by the subcellular distribution of AQP2 protein. |
| T10979 |
16059-16061 |
IN |
denotes |
In |
| T10980 |
16124-16130 |
VB |
denotes |
rescue |
| T10981 |
16062-16065 |
DT |
denotes |
the |
| T10982 |
16077-16082 |
NNS |
denotes |
ducts |
| T10983 |
16066-16076 |
VBG |
denotes |
collecting |
| T10984 |
16083-16087 |
IN |
denotes |
from |
| T10985 |
16088-16097 |
NN |
denotes |
Aqp2F204V |
| T10986 |
16100-16104 |
NNS |
denotes |
mice |
| T10987 |
16097-16098 |
HYPH |
denotes |
/ |
| T10988 |
16098-16099 |
SYM |
denotes |
+ |
| T10989 |
16104-16106 |
, |
denotes |
, |
| T10990 |
16106-16109 |
DT |
denotes |
the |
| T10991 |
16115-16119 |
NN |
denotes |
type |
| T10992 |
16110-16114 |
JJ |
denotes |
wild |
| T10993 |
16120-16123 |
MD |
denotes |
may |
| T10994 |
16131-16134 |
DT |
denotes |
the |
| T10995 |
16142-16149 |
NN |
denotes |
protein |
| T10996 |
16135-16141 |
NN |
denotes |
mutant |
| T10997 |
16150-16152 |
IN |
denotes |
as |
| T10998 |
16153-16162 |
VBN |
denotes |
suggested |
| T10999 |
16163-16165 |
IN |
denotes |
by |
| T11000 |
16166-16169 |
DT |
denotes |
the |
| T11001 |
16182-16194 |
NN |
denotes |
distribution |
| T11002 |
16170-16181 |
JJ |
denotes |
subcellular |
| T11003 |
16195-16197 |
IN |
denotes |
of |
| T11004 |
16198-16202 |
NN |
denotes |
AQP2 |
| T11005 |
16203-16210 |
NN |
denotes |
protein |
| T11006 |
16210-16211 |
. |
denotes |
. |
| T11007 |
16211-16342 |
sentence |
denotes |
To test this idea, we first looked for an interaction between mutant and wild-type proteins in transfected MDCK cells (Figure 5B). |
| T11008 |
16212-16214 |
TO |
denotes |
To |
| T11009 |
16215-16219 |
VB |
denotes |
test |
| T11010 |
16240-16246 |
VBD |
denotes |
looked |
| T11011 |
16220-16224 |
DT |
denotes |
this |
| T11012 |
16225-16229 |
NN |
denotes |
idea |
| T11013 |
16229-16231 |
, |
denotes |
, |
| T11014 |
16231-16233 |
PRP |
denotes |
we |
| T11015 |
16234-16239 |
RB |
denotes |
first |
| T11016 |
16247-16250 |
IN |
denotes |
for |
| T11017 |
16251-16253 |
DT |
denotes |
an |
| T11018 |
16254-16265 |
NN |
denotes |
interaction |
| T11019 |
16266-16273 |
IN |
denotes |
between |
| T11020 |
16274-16280 |
NN |
denotes |
mutant |
| T11021 |
16295-16303 |
NN |
denotes |
proteins |
| T11022 |
16281-16284 |
CC |
denotes |
and |
| T11023 |
16285-16289 |
JJ |
denotes |
wild |
| T11024 |
16290-16294 |
NN |
denotes |
type |
| T11025 |
16289-16290 |
HYPH |
denotes |
- |
| T11026 |
16304-16306 |
IN |
denotes |
in |
| T11027 |
16307-16318 |
VBN |
denotes |
transfected |
| T11028 |
16324-16329 |
NNS |
denotes |
cells |
| T11029 |
16319-16323 |
NN |
denotes |
MDCK |
| T11030 |
16330-16331 |
-LRB- |
denotes |
( |
| T11031 |
16331-16337 |
NN |
denotes |
Figure |
| T11032 |
16338-16340 |
CD |
denotes |
5B |
| T11033 |
16340-16341 |
-RRB- |
denotes |
) |
| T11034 |
16341-16342 |
. |
denotes |
. |
| T11035 |
16342-16508 |
sentence |
denotes |
MDCK cells stably expressing wild-type AQP2 were transiently transfected with GFP expression constructs encoding GFP-tagged wild-type AQP2, AQP2-F204V, or GFP alone. |
| T11036 |
16343-16347 |
NN |
denotes |
MDCK |
| T11037 |
16348-16353 |
NNS |
denotes |
cells |
| T11038 |
16404-16415 |
VBN |
denotes |
transfected |
| T11039 |
16354-16360 |
RB |
denotes |
stably |
| T11040 |
16361-16371 |
VBG |
denotes |
expressing |
| T11041 |
16372-16376 |
JJ |
denotes |
wild |
| T11042 |
16377-16381 |
NN |
denotes |
type |
| T11043 |
16376-16377 |
HYPH |
denotes |
- |
| T11044 |
16382-16386 |
NN |
denotes |
AQP2 |
| T11045 |
16387-16391 |
VBD |
denotes |
were |
| T11046 |
16392-16403 |
RB |
denotes |
transiently |
| T11047 |
16416-16420 |
IN |
denotes |
with |
| T11048 |
16421-16424 |
NN |
denotes |
GFP |
| T11049 |
16436-16446 |
NNS |
denotes |
constructs |
| T11050 |
16425-16435 |
NN |
denotes |
expression |
| T11051 |
16447-16455 |
VBG |
denotes |
encoding |
| T11052 |
16456-16459 |
NN |
denotes |
GFP |
| T11053 |
16460-16466 |
VBN |
denotes |
tagged |
| T11054 |
16459-16460 |
HYPH |
denotes |
- |
| T11055 |
16477-16481 |
NN |
denotes |
AQP2 |
| T11056 |
16467-16471 |
JJ |
denotes |
wild |
| T11057 |
16472-16476 |
NN |
denotes |
type |
| T11058 |
16471-16472 |
HYPH |
denotes |
- |
| T11059 |
16481-16483 |
, |
denotes |
, |
| T11060 |
16483-16487 |
NN |
denotes |
AQP2 |
| T11061 |
16488-16493 |
NN |
denotes |
F204V |
| T11062 |
16487-16488 |
HYPH |
denotes |
- |
| T11063 |
16493-16495 |
, |
denotes |
, |
| T11064 |
16495-16497 |
CC |
denotes |
or |
| T11065 |
16498-16501 |
NN |
denotes |
GFP |
| T11066 |
16502-16507 |
RB |
denotes |
alone |
| T11067 |
16507-16508 |
. |
denotes |
. |
| T11068 |
16508-16758 |
sentence |
denotes |
Antibodies against GFP coimmunoprecipitated wild-type AQP2 when AQP2-GFP or AQP2-F204V-GFP was transiently transfected, but not when GFP by itself was transiently transfected into MDCK cells stably expressing wild-type AQP2 (Figure 5B, upper blots). |
| T11069 |
16509-16519 |
NNS |
denotes |
Antibodies |
| T11070 |
16532-16552 |
VBD |
denotes |
coimmunoprecipitated |
| T11071 |
16520-16527 |
IN |
denotes |
against |
| T11072 |
16528-16531 |
NN |
denotes |
GFP |
| T11073 |
16553-16557 |
JJ |
denotes |
wild |
| T11074 |
16558-16562 |
NN |
denotes |
type |
| T11075 |
16557-16558 |
HYPH |
denotes |
- |
| T11076 |
16563-16567 |
NN |
denotes |
AQP2 |
| T11077 |
16568-16572 |
WRB |
denotes |
when |
| T11078 |
16616-16627 |
VBN |
denotes |
transfected |
| T11079 |
16573-16577 |
NN |
denotes |
AQP2 |
| T11080 |
16578-16581 |
NN |
denotes |
GFP |
| T11081 |
16577-16578 |
HYPH |
denotes |
- |
| T11082 |
16582-16584 |
CC |
denotes |
or |
| T11083 |
16585-16589 |
NN |
denotes |
AQP2 |
| T11084 |
16596-16599 |
NN |
denotes |
GFP |
| T11085 |
16589-16590 |
HYPH |
denotes |
- |
| T11086 |
16590-16595 |
NN |
denotes |
F204V |
| T11087 |
16595-16596 |
HYPH |
denotes |
- |
| T11088 |
16600-16603 |
VBD |
denotes |
was |
| T11089 |
16604-16615 |
RB |
denotes |
transiently |
| T11090 |
16627-16629 |
, |
denotes |
, |
| T11091 |
16629-16632 |
CC |
denotes |
but |
| T11092 |
16633-16636 |
RB |
denotes |
not |
| T11093 |
16637-16641 |
WRB |
denotes |
when |
| T11094 |
16672-16683 |
VBN |
denotes |
transfected |
| T11095 |
16642-16645 |
NN |
denotes |
GFP |
| T11096 |
16646-16648 |
IN |
denotes |
by |
| T11097 |
16649-16655 |
PRP |
denotes |
itself |
| T11098 |
16656-16659 |
VBD |
denotes |
was |
| T11099 |
16660-16671 |
RB |
denotes |
transiently |
| T11100 |
16684-16688 |
IN |
denotes |
into |
| T11101 |
16689-16693 |
NN |
denotes |
MDCK |
| T11102 |
16694-16699 |
NNS |
denotes |
cells |
| T11103 |
16700-16706 |
RB |
denotes |
stably |
| T11104 |
16707-16717 |
VBG |
denotes |
expressing |
| T11105 |
16718-16722 |
JJ |
denotes |
wild |
| T11106 |
16723-16727 |
NN |
denotes |
type |
| T11107 |
16722-16723 |
HYPH |
denotes |
- |
| T11108 |
16728-16732 |
NN |
denotes |
AQP2 |
| T11109 |
16733-16734 |
-LRB- |
denotes |
( |
| T11110 |
16751-16756 |
NNS |
denotes |
blots |
| T11111 |
16734-16740 |
NN |
denotes |
Figure |
| T11112 |
16741-16743 |
CD |
denotes |
5B |
| T11113 |
16743-16745 |
, |
denotes |
, |
| T11114 |
16745-16750 |
JJ |
denotes |
upper |
| T11115 |
16756-16757 |
-RRB- |
denotes |
) |
| T11116 |
16757-16758 |
. |
denotes |
. |
| T11117 |
16758-16888 |
sentence |
denotes |
Western blot of total membranes showed that wild-type AQP2 is equivalently expressed in all three cases (Figure 5B, lower blots). |
| T11118 |
16759-16766 |
NNP |
denotes |
Western |
| T11119 |
16767-16771 |
NN |
denotes |
blot |
| T11120 |
16791-16797 |
VBD |
denotes |
showed |
| T11121 |
16772-16774 |
IN |
denotes |
of |
| T11122 |
16775-16780 |
JJ |
denotes |
total |
| T11123 |
16781-16790 |
NNS |
denotes |
membranes |
| T11124 |
16798-16802 |
IN |
denotes |
that |
| T11125 |
16834-16843 |
VBN |
denotes |
expressed |
| T11126 |
16803-16807 |
JJ |
denotes |
wild |
| T11127 |
16808-16812 |
NN |
denotes |
type |
| T11128 |
16807-16808 |
HYPH |
denotes |
- |
| T11129 |
16813-16817 |
NN |
denotes |
AQP2 |
| T11130 |
16818-16820 |
VBZ |
denotes |
is |
| T11131 |
16821-16833 |
RB |
denotes |
equivalently |
| T11132 |
16844-16846 |
IN |
denotes |
in |
| T11133 |
16847-16850 |
DT |
denotes |
all |
| T11134 |
16857-16862 |
NNS |
denotes |
cases |
| T11135 |
16851-16856 |
CD |
denotes |
three |
| T11136 |
16863-16864 |
-LRB- |
denotes |
( |
| T11137 |
16881-16886 |
NNS |
denotes |
blots |
| T11138 |
16864-16870 |
NN |
denotes |
Figure |
| T11139 |
16871-16873 |
CD |
denotes |
5B |
| T11140 |
16873-16875 |
, |
denotes |
, |
| T11141 |
16875-16880 |
JJ |
denotes |
lower |
| T11142 |
16886-16887 |
-RRB- |
denotes |
) |
| T11143 |
16887-16888 |
. |
denotes |
. |
| T11144 |
16888-17034 |
sentence |
denotes |
If wild-type and mutant proteins are indeed interacting in the cell, is this interaction sufficient to rescue the localization of mutant protein? |
| T11145 |
16889-16891 |
IN |
denotes |
If |
| T11146 |
16933-16944 |
VBG |
denotes |
interacting |
| T11147 |
16892-16896 |
JJ |
denotes |
wild |
| T11148 |
16897-16901 |
NN |
denotes |
type |
| T11149 |
16896-16897 |
HYPH |
denotes |
- |
| T11150 |
16913-16921 |
NN |
denotes |
proteins |
| T11151 |
16902-16905 |
CC |
denotes |
and |
| T11152 |
16906-16912 |
NN |
denotes |
mutant |
| T11153 |
16922-16925 |
VBP |
denotes |
are |
| T11154 |
16926-16932 |
RB |
denotes |
indeed |
| T11155 |
16958-16960 |
VBZ |
denotes |
is |
| T11156 |
16945-16947 |
IN |
denotes |
in |
| T11157 |
16948-16951 |
DT |
denotes |
the |
| T11158 |
16952-16956 |
NN |
denotes |
cell |
| T11159 |
16956-16958 |
, |
denotes |
, |
| T11160 |
16961-16965 |
DT |
denotes |
this |
| T11161 |
16966-16977 |
NN |
denotes |
interaction |
| T11162 |
16978-16988 |
JJ |
denotes |
sufficient |
| T11163 |
16989-16991 |
TO |
denotes |
to |
| T11164 |
16992-16998 |
VB |
denotes |
rescue |
| T11165 |
16999-17002 |
DT |
denotes |
the |
| T11166 |
17003-17015 |
NN |
denotes |
localization |
| T11167 |
17016-17018 |
IN |
denotes |
of |
| T11168 |
17019-17025 |
NN |
denotes |
mutant |
| T11169 |
17026-17033 |
NN |
denotes |
protein |
| T11170 |
17033-17034 |
. |
denotes |
? |
| T11171 |
17034-17157 |
sentence |
denotes |
To answer this question, we used MDCK cells stably transfected with wild-type AQP2 expressing vector or with empty vector. |
| T11172 |
17035-17037 |
TO |
denotes |
To |
| T11173 |
17038-17044 |
VB |
denotes |
answer |
| T11174 |
17063-17067 |
VBD |
denotes |
used |
| T11175 |
17045-17049 |
DT |
denotes |
this |
| T11176 |
17050-17058 |
NN |
denotes |
question |
| T11177 |
17058-17060 |
, |
denotes |
, |
| T11178 |
17060-17062 |
PRP |
denotes |
we |
| T11179 |
17068-17072 |
NN |
denotes |
MDCK |
| T11180 |
17073-17078 |
NNS |
denotes |
cells |
| T11181 |
17079-17085 |
RB |
denotes |
stably |
| T11182 |
17086-17097 |
VBN |
denotes |
transfected |
| T11183 |
17098-17102 |
IN |
denotes |
with |
| T11184 |
17103-17107 |
JJ |
denotes |
wild |
| T11185 |
17108-17112 |
NN |
denotes |
type |
| T11186 |
17107-17108 |
HYPH |
denotes |
- |
| T11187 |
17113-17117 |
NN |
denotes |
AQP2 |
| T11188 |
17118-17128 |
VBG |
denotes |
expressing |
| T11189 |
17129-17135 |
NN |
denotes |
vector |
| T11190 |
17136-17138 |
CC |
denotes |
or |
| T11191 |
17139-17143 |
IN |
denotes |
with |
| T11192 |
17144-17149 |
JJ |
denotes |
empty |
| T11193 |
17150-17156 |
NN |
denotes |
vector |
| T11194 |
17156-17157 |
. |
denotes |
. |
| T11195 |
17157-17251 |
sentence |
denotes |
On top of these, we transiently transfected AQP2-GFP or AQP2-F204V-GFP expression constructs. |
| T11196 |
17158-17160 |
IN |
denotes |
On |
| T11197 |
17190-17201 |
VBD |
denotes |
transfected |
| T11198 |
17161-17164 |
NN |
denotes |
top |
| T11199 |
17165-17167 |
IN |
denotes |
of |
| T11200 |
17168-17173 |
DT |
denotes |
these |
| T11201 |
17173-17175 |
, |
denotes |
, |
| T11202 |
17175-17177 |
PRP |
denotes |
we |
| T11203 |
17178-17189 |
RB |
denotes |
transiently |
| T11204 |
17202-17206 |
NN |
denotes |
AQP2 |
| T11205 |
17207-17210 |
NN |
denotes |
GFP |
| T11206 |
17206-17207 |
HYPH |
denotes |
- |
| T11207 |
17240-17250 |
NNS |
denotes |
constructs |
| T11208 |
17211-17213 |
CC |
denotes |
or |
| T11209 |
17214-17218 |
NN |
denotes |
AQP2 |
| T11210 |
17225-17228 |
NN |
denotes |
GFP |
| T11211 |
17218-17219 |
HYPH |
denotes |
- |
| T11212 |
17219-17224 |
NN |
denotes |
F204V |
| T11213 |
17224-17225 |
HYPH |
denotes |
- |
| T11214 |
17229-17239 |
NN |
denotes |
expression |
| T11215 |
17250-17251 |
. |
denotes |
. |
| T11216 |
17251-17467 |
sentence |
denotes |
AQP2-GFP localized to the apical surface upon forskolin stimulation whether it was transiently transfected into vector-only cells (Figure 5C, upper left images) or into wild-type AQP2 cells (Figure 5C, upper right). |
| T11217 |
17252-17256 |
NN |
denotes |
AQP2 |
| T11218 |
17257-17260 |
NN |
denotes |
GFP |
| T11219 |
17256-17257 |
HYPH |
denotes |
- |
| T11220 |
17261-17270 |
VBD |
denotes |
localized |
| T11221 |
17271-17273 |
IN |
denotes |
to |
| T11222 |
17274-17277 |
DT |
denotes |
the |
| T11223 |
17285-17292 |
NN |
denotes |
surface |
| T11224 |
17278-17284 |
JJ |
denotes |
apical |
| T11225 |
17293-17297 |
IN |
denotes |
upon |
| T11226 |
17298-17307 |
NN |
denotes |
forskolin |
| T11227 |
17308-17319 |
NN |
denotes |
stimulation |
| T11228 |
17320-17327 |
IN |
denotes |
whether |
| T11229 |
17347-17358 |
VBN |
denotes |
transfected |
| T11230 |
17328-17330 |
PRP |
denotes |
it |
| T11231 |
17331-17334 |
VBD |
denotes |
was |
| T11232 |
17335-17346 |
RB |
denotes |
transiently |
| T11233 |
17359-17363 |
IN |
denotes |
into |
| T11234 |
17364-17370 |
NN |
denotes |
vector |
| T11235 |
17371-17375 |
JJ |
denotes |
only |
| T11236 |
17370-17371 |
HYPH |
denotes |
- |
| T11237 |
17376-17381 |
NNS |
denotes |
cells |
| T11238 |
17382-17383 |
-LRB- |
denotes |
( |
| T11239 |
17405-17411 |
NNS |
denotes |
images |
| T11240 |
17383-17389 |
NN |
denotes |
Figure |
| T11241 |
17390-17392 |
CD |
denotes |
5C |
| T11242 |
17392-17394 |
, |
denotes |
, |
| T11243 |
17394-17399 |
JJ |
denotes |
upper |
| T11244 |
17400-17404 |
JJ |
denotes |
left |
| T11245 |
17411-17412 |
-RRB- |
denotes |
) |
| T11246 |
17413-17415 |
CC |
denotes |
or |
| T11247 |
17416-17420 |
IN |
denotes |
into |
| T11248 |
17421-17425 |
JJ |
denotes |
wild |
| T11249 |
17426-17430 |
NN |
denotes |
type |
| T11250 |
17425-17426 |
HYPH |
denotes |
- |
| T11251 |
17436-17441 |
NNS |
denotes |
cells |
| T11252 |
17431-17435 |
NN |
denotes |
AQP2 |
| T11253 |
17442-17443 |
-LRB- |
denotes |
( |
| T11254 |
17443-17449 |
NN |
denotes |
Figure |
| T11255 |
17450-17452 |
CD |
denotes |
5C |
| T11256 |
17452-17454 |
, |
denotes |
, |
| T11257 |
17454-17459 |
JJ |
denotes |
upper |
| T11258 |
17460-17465 |
JJ |
denotes |
right |
| T11259 |
17465-17466 |
-RRB- |
denotes |
) |
| T11260 |
17466-17467 |
. |
denotes |
. |
| T11261 |
17467-17623 |
sentence |
denotes |
AQP2-F204V-GFP, when expressed by transient transfection into vector only cells, showed a diffuse cytoplasmic distribution pattern (Figure 5C, lower left). |
| T11262 |
17468-17472 |
NN |
denotes |
AQP2 |
| T11263 |
17479-17482 |
NN |
denotes |
GFP |
| T11264 |
17472-17473 |
HYPH |
denotes |
- |
| T11265 |
17473-17478 |
NN |
denotes |
F204V |
| T11266 |
17478-17479 |
HYPH |
denotes |
- |
| T11267 |
17549-17555 |
VBD |
denotes |
showed |
| T11268 |
17482-17484 |
, |
denotes |
, |
| T11269 |
17484-17488 |
WRB |
denotes |
when |
| T11270 |
17489-17498 |
VBN |
denotes |
expressed |
| T11271 |
17499-17501 |
IN |
denotes |
by |
| T11272 |
17502-17511 |
JJ |
denotes |
transient |
| T11273 |
17512-17524 |
NN |
denotes |
transfection |
| T11274 |
17525-17529 |
IN |
denotes |
into |
| T11275 |
17530-17536 |
NN |
denotes |
vector |
| T11276 |
17537-17541 |
JJ |
denotes |
only |
| T11277 |
17542-17547 |
NNS |
denotes |
cells |
| T11278 |
17547-17549 |
, |
denotes |
, |
| T11279 |
17556-17557 |
DT |
denotes |
a |
| T11280 |
17591-17598 |
NN |
denotes |
pattern |
| T11281 |
17558-17565 |
JJ |
denotes |
diffuse |
| T11282 |
17566-17577 |
JJ |
denotes |
cytoplasmic |
| T11283 |
17578-17590 |
NN |
denotes |
distribution |
| T11293 |
17624-17628 |
WRB |
denotes |
When |
| T11294 |
17629-17638 |
VBN |
denotes |
expressed |
| T11295 |
17688-17697 |
VBD |
denotes |
localized |
| T11296 |
17639-17641 |
IN |
denotes |
in |
| T11297 |
17642-17646 |
JJ |
denotes |
wild |
| T11298 |
17647-17651 |
NN |
denotes |
type |
| T11299 |
17646-17647 |
HYPH |
denotes |
- |
| T11300 |
17657-17662 |
NNS |
denotes |
cells |
| T11301 |
17652-17656 |
NN |
denotes |
AQP2 |
| T11302 |
17662-17664 |
, |
denotes |
, |
| T11303 |
17664-17671 |
RB |
denotes |
however |
| T11304 |
17671-17673 |
, |
denotes |
, |
| T11305 |
17673-17677 |
NN |
denotes |
AQP2 |
| T11306 |
17684-17687 |
NN |
denotes |
GFP |
| T11307 |
17677-17678 |
HYPH |
denotes |
- |
| T11308 |
17678-17683 |
NN |
denotes |
F204V |
| T11309 |
17683-17684 |
HYPH |
denotes |
- |
| T11310 |
17698-17700 |
IN |
denotes |
to |
| T11311 |
17701-17704 |
DT |
denotes |
the |
| T11312 |
17712-17719 |
NN |
denotes |
surface |
| T11313 |
17705-17711 |
JJ |
denotes |
apical |
| T11314 |
17720-17722 |
IN |
denotes |
to |
| T11315 |
17723-17730 |
VBG |
denotes |
varying |
| T11316 |
17731-17738 |
NNS |
denotes |
degrees |
| T11317 |
17739-17740 |
-LRB- |
denotes |
( |
| T11318 |
17763-17769 |
NNS |
denotes |
images |
| T11319 |
17740-17746 |
NN |
denotes |
Figure |
| T11320 |
17747-17749 |
CD |
denotes |
5C |
| T11321 |
17749-17751 |
, |
denotes |
, |
| T11322 |
17751-17756 |
JJ |
denotes |
lower |
| T11323 |
17757-17762 |
JJ |
denotes |
right |
| T11324 |
17770-17771 |
-LRB- |
denotes |
[ |
| T11325 |
17771-17772 |
CD |
denotes |
i |
| T11326 |
17772-17773 |
SYM |
denotes |
– |
| T11327 |
17773-17776 |
CD |
denotes |
iii |
| T11328 |
17776-17777 |
-RRB- |
denotes |
] |
| T11329 |
17777-17778 |
-RRB- |
denotes |
) |
| T11330 |
17778-17779 |
. |
denotes |
. |
| T11331 |
17779-17861 |
sentence |
denotes |
The lower right images of Figure 5C shows three cells from a single transfection. |
| T11332 |
17780-17783 |
DT |
denotes |
The |
| T11333 |
17796-17802 |
NNS |
denotes |
images |
| T11334 |
17784-17789 |
JJ |
denotes |
lower |
| T11335 |
17790-17795 |
JJ |
denotes |
right |
| T11336 |
17816-17821 |
VBZ |
denotes |
shows |
| T11337 |
17803-17805 |
IN |
denotes |
of |
| T11338 |
17806-17812 |
NN |
denotes |
Figure |
| T11339 |
17813-17815 |
CD |
denotes |
5C |
| T11340 |
17822-17827 |
CD |
denotes |
three |
| T11341 |
17828-17833 |
NNS |
denotes |
cells |
| T11342 |
17834-17838 |
IN |
denotes |
from |
| T11343 |
17839-17840 |
DT |
denotes |
a |
| T11344 |
17848-17860 |
NN |
denotes |
transfection |
| T11345 |
17841-17847 |
JJ |
denotes |
single |
| T11346 |
17860-17861 |
. |
denotes |
. |
| T11347 |
17861-18009 |
sentence |
denotes |
The first is a nontransfected cell that shows the localization of the stably expressing wild-type AQP2, which is apical upon forskolin stimulation. |
| T11348 |
17862-17865 |
DT |
denotes |
The |
| T11349 |
17866-17871 |
JJ |
denotes |
first |
| T11350 |
17872-17874 |
VBZ |
denotes |
is |
| T11351 |
17875-17876 |
DT |
denotes |
a |
| T11352 |
17892-17896 |
NN |
denotes |
cell |
| T11353 |
17877-17891 |
JJ |
denotes |
nontransfected |
| T11354 |
17897-17901 |
WDT |
denotes |
that |
| T11355 |
17902-17907 |
VBZ |
denotes |
shows |
| T11356 |
17908-17911 |
DT |
denotes |
the |
| T11357 |
17912-17924 |
NN |
denotes |
localization |
| T11358 |
17925-17927 |
IN |
denotes |
of |
| T11359 |
17928-17931 |
DT |
denotes |
the |
| T11360 |
17960-17964 |
NN |
denotes |
AQP2 |
| T11361 |
17932-17938 |
RB |
denotes |
stably |
| T11362 |
17939-17949 |
VBG |
denotes |
expressing |
| T11363 |
17950-17954 |
JJ |
denotes |
wild |
| T11364 |
17955-17959 |
NN |
denotes |
type |
| T11365 |
17954-17955 |
HYPH |
denotes |
- |
| T11366 |
17964-17966 |
, |
denotes |
, |
| T11367 |
17966-17971 |
WDT |
denotes |
which |
| T11368 |
17972-17974 |
VBZ |
denotes |
is |
| T11369 |
17975-17981 |
JJ |
denotes |
apical |
| T11370 |
17982-17986 |
IN |
denotes |
upon |
| T11371 |
17987-17996 |
NN |
denotes |
forskolin |
| T11372 |
17997-18008 |
NN |
denotes |
stimulation |
| T11373 |
18008-18009 |
. |
denotes |
. |
| T11374 |
18009-18106 |
sentence |
denotes |
The next two show expression of both the stable wild-type AQP2 and the transient AQP2-F204V-GFP. |
| T11375 |
18010-18013 |
DT |
denotes |
The |
| T11376 |
18019-18022 |
CD |
denotes |
two |
| T11377 |
18014-18018 |
JJ |
denotes |
next |
| T11378 |
18023-18027 |
VBP |
denotes |
show |
| T11379 |
18028-18038 |
NN |
denotes |
expression |
| T11380 |
18039-18041 |
IN |
denotes |
of |
| T11381 |
18042-18046 |
CC |
denotes |
both |
| T11382 |
18068-18072 |
NN |
denotes |
AQP2 |
| T11383 |
18047-18050 |
DT |
denotes |
the |
| T11384 |
18051-18057 |
JJ |
denotes |
stable |
| T11385 |
18058-18062 |
JJ |
denotes |
wild |
| T11386 |
18063-18067 |
NN |
denotes |
type |
| T11387 |
18062-18063 |
HYPH |
denotes |
- |
| T11388 |
18073-18076 |
CC |
denotes |
and |
| T11389 |
18077-18080 |
DT |
denotes |
the |
| T11390 |
18102-18105 |
NN |
denotes |
GFP |
| T11391 |
18081-18090 |
JJ |
denotes |
transient |
| T11392 |
18091-18095 |
NN |
denotes |
AQP2 |
| T11393 |
18095-18096 |
HYPH |
denotes |
- |
| T11394 |
18096-18101 |
NN |
denotes |
F204V |
| T11395 |
18101-18102 |
HYPH |
denotes |
- |
| T11396 |
18105-18106 |
. |
denotes |
. |
| T11397 |
18106-18239 |
sentence |
denotes |
In cell (ii), localization of wild-type AQP2 was indistinguishable from AQP2-F204V-GFP; both were apical upon forskolin stimulation. |
| T11398 |
18107-18109 |
IN |
denotes |
In |
| T11399 |
18152-18155 |
VBD |
denotes |
was |
| T11400 |
18110-18114 |
NN |
denotes |
cell |
| T11401 |
18115-18116 |
-LRB- |
denotes |
( |
| T11402 |
18116-18118 |
CD |
denotes |
ii |
| T11403 |
18118-18119 |
-RRB- |
denotes |
) |
| T11404 |
18119-18121 |
, |
denotes |
, |
| T11405 |
18121-18133 |
NN |
denotes |
localization |
| T11406 |
18134-18136 |
IN |
denotes |
of |
| T11407 |
18137-18141 |
JJ |
denotes |
wild |
| T11408 |
18142-18146 |
NN |
denotes |
type |
| T11409 |
18141-18142 |
HYPH |
denotes |
- |
| T11410 |
18147-18151 |
NN |
denotes |
AQP2 |
| T11411 |
18200-18204 |
VBD |
denotes |
were |
| T11412 |
18156-18173 |
JJ |
denotes |
indistinguishable |
| T11413 |
18174-18178 |
IN |
denotes |
from |
| T11414 |
18179-18183 |
NN |
denotes |
AQP2 |
| T11415 |
18190-18193 |
NN |
denotes |
GFP |
| T11416 |
18183-18184 |
HYPH |
denotes |
- |
| T11417 |
18184-18189 |
NN |
denotes |
F204V |
| T11418 |
18189-18190 |
HYPH |
denotes |
- |
| T11419 |
18193-18194 |
: |
denotes |
; |
| T11420 |
18195-18199 |
DT |
denotes |
both |
| T11421 |
18205-18211 |
JJ |
denotes |
apical |
| T11422 |
18212-18216 |
IN |
denotes |
upon |
| T11423 |
18217-18226 |
NN |
denotes |
forskolin |
| T11424 |
18227-18238 |
NN |
denotes |
stimulation |
| T11425 |
18238-18239 |
. |
denotes |
. |
| T11426 |
18239-18344 |
sentence |
denotes |
Although the effect was subtle in cell (iii), AQP2-F204V-GFP was partly localized to the apical surface. |
| T11427 |
18240-18248 |
IN |
denotes |
Although |
| T11428 |
18260-18263 |
VBD |
denotes |
was |
| T11429 |
18249-18252 |
DT |
denotes |
the |
| T11430 |
18253-18259 |
NN |
denotes |
effect |
| T11431 |
18312-18321 |
VBN |
denotes |
localized |
| T11432 |
18264-18270 |
JJ |
denotes |
subtle |
| T11433 |
18271-18273 |
IN |
denotes |
in |
| T11434 |
18274-18278 |
NN |
denotes |
cell |
| T11435 |
18279-18280 |
-LRB- |
denotes |
( |
| T11436 |
18280-18283 |
CD |
denotes |
iii |
| T11437 |
18283-18284 |
-RRB- |
denotes |
) |
| T11438 |
18284-18286 |
, |
denotes |
, |
| T11439 |
18286-18290 |
NN |
denotes |
AQP2 |
| T11440 |
18297-18300 |
NN |
denotes |
GFP |
| T11441 |
18290-18291 |
HYPH |
denotes |
- |
| T11442 |
18291-18296 |
NN |
denotes |
F204V |
| T11443 |
18296-18297 |
HYPH |
denotes |
- |
| T11444 |
18301-18304 |
VBD |
denotes |
was |
| T11445 |
18305-18311 |
RB |
denotes |
partly |
| T11446 |
18322-18324 |
IN |
denotes |
to |
| T11447 |
18325-18328 |
DT |
denotes |
the |
| T11448 |
18336-18343 |
NN |
denotes |
surface |
| T11449 |
18329-18335 |
JJ |
denotes |
apical |
| T11450 |
18343-18344 |
. |
denotes |
. |
| T11451 |
18344-18454 |
sentence |
denotes |
Generally, the localization of AQP2-F204V-GFP was clearly more apical when wild-type AQP2 was also expressed. |
| T11452 |
18345-18354 |
RB |
denotes |
Generally |
| T11453 |
18391-18394 |
VBD |
denotes |
was |
| T11454 |
18354-18356 |
, |
denotes |
, |
| T11455 |
18356-18359 |
DT |
denotes |
the |
| T11456 |
18360-18372 |
NN |
denotes |
localization |
| T11457 |
18373-18375 |
IN |
denotes |
of |
| T11458 |
18376-18380 |
NN |
denotes |
AQP2 |
| T11459 |
18387-18390 |
NN |
denotes |
GFP |
| T11460 |
18380-18381 |
HYPH |
denotes |
- |
| T11461 |
18381-18386 |
NN |
denotes |
F204V |
| T11462 |
18386-18387 |
HYPH |
denotes |
- |
| T11463 |
18395-18402 |
RB |
denotes |
clearly |
| T11464 |
18403-18407 |
RBR |
denotes |
more |
| T11465 |
18408-18414 |
JJ |
denotes |
apical |
| T11466 |
18415-18419 |
WRB |
denotes |
when |
| T11467 |
18444-18453 |
VBN |
denotes |
expressed |
| T11468 |
18420-18424 |
JJ |
denotes |
wild |
| T11469 |
18425-18429 |
NN |
denotes |
type |
| T11470 |
18424-18425 |
HYPH |
denotes |
- |
| T11471 |
18430-18434 |
NN |
denotes |
AQP2 |
| T11472 |
18435-18438 |
VBD |
denotes |
was |
| T11473 |
18439-18443 |
RB |
denotes |
also |
| T11474 |
18453-18454 |
. |
denotes |
. |
| T11475 |
18454-18687 |
sentence |
denotes |
To confirm these results biochemically, we transfected the same cell lines (wild-type AQP2 or vector) with F204V-GFP, biotinylated surface proteins after forskolin stimulation, and precipitated the biotinylated proteins (Figure 5D). |
| T11476 |
18455-18457 |
TO |
denotes |
To |
| T11477 |
18458-18465 |
VB |
denotes |
confirm |
| T11478 |
18498-18509 |
VBD |
denotes |
transfected |
| T11479 |
18466-18471 |
DT |
denotes |
these |
| T11480 |
18472-18479 |
NNS |
denotes |
results |
| T11481 |
18480-18493 |
RB |
denotes |
biochemically |
| T11482 |
18493-18495 |
, |
denotes |
, |
| T11483 |
18495-18497 |
PRP |
denotes |
we |
| T11484 |
18510-18513 |
DT |
denotes |
the |
| T11485 |
18524-18529 |
NNS |
denotes |
lines |
| T11486 |
18514-18518 |
JJ |
denotes |
same |
| T11487 |
18519-18523 |
NN |
denotes |
cell |
| T11488 |
18530-18531 |
-LRB- |
denotes |
( |
| T11489 |
18531-18535 |
JJ |
denotes |
wild |
| T11490 |
18536-18540 |
NN |
denotes |
type |
| T11491 |
18535-18536 |
HYPH |
denotes |
- |
| T11492 |
18541-18545 |
NN |
denotes |
AQP2 |
| T11493 |
18546-18548 |
CC |
denotes |
or |
| T11494 |
18549-18555 |
NN |
denotes |
vector |
| T11495 |
18555-18556 |
-RRB- |
denotes |
) |
| T11496 |
18557-18561 |
IN |
denotes |
with |
| T11497 |
18562-18567 |
NN |
denotes |
F204V |
| T11498 |
18568-18571 |
NN |
denotes |
GFP |
| T11499 |
18567-18568 |
HYPH |
denotes |
- |
| T11500 |
18571-18573 |
, |
denotes |
, |
| T11501 |
18573-18585 |
VBD |
denotes |
biotinylated |
| T11502 |
18586-18593 |
NN |
denotes |
surface |
| T11503 |
18594-18602 |
NN |
denotes |
proteins |
| T11504 |
18603-18608 |
IN |
denotes |
after |
| T11505 |
18609-18618 |
NN |
denotes |
forskolin |
| T11506 |
18619-18630 |
NN |
denotes |
stimulation |
| T11507 |
18630-18632 |
, |
denotes |
, |
| T11508 |
18632-18635 |
CC |
denotes |
and |
| T11509 |
18636-18648 |
VBD |
denotes |
precipitated |
| T11510 |
18649-18652 |
DT |
denotes |
the |
| T11511 |
18666-18674 |
NN |
denotes |
proteins |
| T11512 |
18653-18665 |
VBN |
denotes |
biotinylated |
| T11513 |
18675-18676 |
-LRB- |
denotes |
( |
| T11514 |
18676-18682 |
NN |
denotes |
Figure |
| T11515 |
18683-18685 |
CD |
denotes |
5D |
| T11516 |
18685-18686 |
-RRB- |
denotes |
) |
| T11517 |
18686-18687 |
. |
denotes |
. |
| T11518 |
18687-18880 |
sentence |
denotes |
AQP2-F204V-GFP is expressed approximately equally in both cell lines (Figure 5D, total cells), but is biotinylated only when wild-type AQP2 is also expressed (Figure 5D, surface biotinylated). |
| T11519 |
18688-18692 |
NN |
denotes |
AQP2 |
| T11520 |
18699-18702 |
NN |
denotes |
GFP |
| T11521 |
18692-18693 |
HYPH |
denotes |
- |
| T11522 |
18693-18698 |
NN |
denotes |
F204V |
| T11523 |
18698-18699 |
HYPH |
denotes |
- |
| T11524 |
18706-18715 |
VBN |
denotes |
expressed |
| T11525 |
18703-18705 |
VBZ |
denotes |
is |
| T11526 |
18716-18729 |
RB |
denotes |
approximately |
| T11527 |
18730-18737 |
RB |
denotes |
equally |
| T11528 |
18738-18740 |
IN |
denotes |
in |
| T11529 |
18741-18745 |
DT |
denotes |
both |
| T11530 |
18751-18756 |
NNS |
denotes |
lines |
| T11531 |
18746-18750 |
NN |
denotes |
cell |
| T11532 |
18757-18758 |
-LRB- |
denotes |
( |
| T11533 |
18775-18780 |
NNS |
denotes |
cells |
| T11534 |
18758-18764 |
NN |
denotes |
Figure |
| T11535 |
18765-18767 |
CD |
denotes |
5D |
| T11536 |
18767-18769 |
, |
denotes |
, |
| T11537 |
18769-18774 |
JJ |
denotes |
total |
| T11538 |
18780-18781 |
-RRB- |
denotes |
) |
| T11539 |
18781-18783 |
, |
denotes |
, |
| T11540 |
18783-18786 |
CC |
denotes |
but |
| T11541 |
18787-18789 |
VBZ |
denotes |
is |
| T11542 |
18790-18802 |
VBN |
denotes |
biotinylated |
| T11543 |
18803-18807 |
RB |
denotes |
only |
| T11544 |
18836-18845 |
VBN |
denotes |
expressed |
| T11545 |
18808-18812 |
WRB |
denotes |
when |
| T11546 |
18813-18817 |
JJ |
denotes |
wild |
| T11547 |
18818-18822 |
NN |
denotes |
type |
| T11548 |
18817-18818 |
HYPH |
denotes |
- |
| T11549 |
18823-18827 |
NN |
denotes |
AQP2 |
| T11550 |
18828-18830 |
VBZ |
denotes |
is |
| T11551 |
18831-18835 |
RB |
denotes |
also |
| T11552 |
18846-18847 |
-LRB- |
denotes |
( |
| T11553 |
18847-18853 |
NN |
denotes |
Figure |
| T11554 |
18854-18856 |
CD |
denotes |
5D |
| T11555 |
18856-18858 |
, |
denotes |
, |
| T11556 |
18858-18865 |
NN |
denotes |
surface |
| T11557 |
18866-18878 |
VBN |
denotes |
biotinylated |
| T11558 |
18878-18879 |
-RRB- |
denotes |
) |
| T11559 |
18879-18880 |
. |
denotes |
. |
| T11560 |
18880-19065 |
sentence |
denotes |
Since only cell surface proteins are accessible to biotin, these results indicate that AQP2-F204V is transported to the cell surface when wild-type AQP2 is present, but not on its own. |
| T11561 |
18881-18886 |
IN |
denotes |
Since |
| T11562 |
18914-18917 |
VBP |
denotes |
are |
| T11563 |
18887-18891 |
RB |
denotes |
only |
| T11564 |
18905-18913 |
NN |
denotes |
proteins |
| T11565 |
18892-18896 |
NN |
denotes |
cell |
| T11566 |
18897-18904 |
NN |
denotes |
surface |
| T11567 |
18954-18962 |
VBP |
denotes |
indicate |
| T11568 |
18918-18928 |
JJ |
denotes |
accessible |
| T11569 |
18929-18931 |
IN |
denotes |
to |
| T11570 |
18932-18938 |
NN |
denotes |
biotin |
| T11571 |
18938-18940 |
, |
denotes |
, |
| T11572 |
18940-18945 |
DT |
denotes |
these |
| T11573 |
18946-18953 |
NNS |
denotes |
results |
| T11574 |
18963-18967 |
IN |
denotes |
that |
| T11575 |
18982-18993 |
VBN |
denotes |
transported |
| T11576 |
18968-18972 |
NN |
denotes |
AQP2 |
| T11577 |
18973-18978 |
NN |
denotes |
F204V |
| T11578 |
18972-18973 |
HYPH |
denotes |
- |
| T11579 |
18979-18981 |
VBZ |
denotes |
is |
| T11580 |
18994-18996 |
IN |
denotes |
to |
| T11581 |
18997-19000 |
DT |
denotes |
the |
| T11582 |
19006-19013 |
NN |
denotes |
surface |
| T11583 |
19001-19005 |
NN |
denotes |
cell |
| T11584 |
19014-19018 |
WRB |
denotes |
when |
| T11585 |
19034-19036 |
VBZ |
denotes |
is |
| T11586 |
19019-19023 |
JJ |
denotes |
wild |
| T11587 |
19024-19028 |
NN |
denotes |
type |
| T11588 |
19023-19024 |
HYPH |
denotes |
- |
| T11589 |
19029-19033 |
NN |
denotes |
AQP2 |
| T11590 |
19037-19044 |
JJ |
denotes |
present |
| T11591 |
19044-19046 |
, |
denotes |
, |
| T11592 |
19046-19049 |
CC |
denotes |
but |
| T11593 |
19050-19053 |
RB |
denotes |
not |
| T11594 |
19054-19056 |
IN |
denotes |
on |
| T11595 |
19057-19060 |
PRP$ |
denotes |
its |
| T11596 |
19061-19064 |
JJ |
denotes |
own |
| T11597 |
19064-19065 |
. |
denotes |
. |
| R916 |
T8980 |
T8981 |
punct |
[,20 |
| R917 |
T8951 |
T8952 |
prep |
In,found |
| R918 |
T8953 |
T8954 |
det |
a,screen |
| R919 |
T8954 |
T8951 |
pobj |
screen,In |
| R920 |
T8955 |
T8956 |
amod |
forward,genetic |
| R921 |
T8956 |
T8954 |
amod |
genetic,screen |
| R922 |
T8957 |
T8958 |
dep |
that,used |
| R923 |
T8958 |
T8954 |
relcl |
used,screen |
| R924 |
T8959 |
T8958 |
dobj |
ethylnitrosourea,used |
| R925 |
T8960 |
T8959 |
punct |
(,ethylnitrosourea |
| R926 |
T8961 |
T8959 |
appos |
ENU,ethylnitrosourea |
| R927 |
T8981 |
T8958 |
parataxis |
20,used |
| R928 |
T8962 |
T8958 |
punct |
),used |
| R929 |
T8963 |
T8964 |
aux |
to,induce |
| R930 |
T8964 |
T8958 |
advcl |
induce,used |
| R931 |
T8982 |
T8981 |
nummod |
19,20 |
| R932 |
T8965 |
T8964 |
dobj |
mutations,induce |
| R933 |
T8966 |
T8964 |
prep |
in,induce |
| R934 |
T8967 |
T8968 |
det |
a,animal |
| R935 |
T8983 |
T8981 |
punct |
",",20 |
| R936 |
T8968 |
T8966 |
pobj |
animal,in |
| R937 |
T8969 |
T8968 |
compound |
founder,animal |
| R938 |
T8970 |
T8971 |
poss |
whose,offspring |
| R939 |
T8984 |
T8981 |
punct |
],20 |
| R940 |
T8971 |
T8972 |
dep |
offspring,screened |
| R941 |
T8972 |
T8968 |
relcl |
screened,animal |
| R942 |
T8973 |
T8972 |
auxpass |
were,screened |
| R943 |
T8985 |
T8952 |
punct |
", ",found |
| R944 |
T8974 |
T8972 |
advmod |
then,screened |
| R945 |
T8986 |
T8952 |
nsubj |
we,found |
| R946 |
T8975 |
T8972 |
prep |
for,screened |
| R947 |
T8976 |
T8977 |
amod |
abnormal,metabolism |
| R948 |
T8987 |
T8988 |
det |
a,family |
| R949 |
T8977 |
T8975 |
pobj |
metabolism,for |
| R950 |
T8978 |
T8979 |
amod |
whole,body |
| R951 |
T8988 |
T8952 |
dobj |
family,found |
| R952 |
T8979 |
T8977 |
compound |
body,metabolism |
| R953 |
T8989 |
T8988 |
prep |
of,family |
| R954 |
T8990 |
T8989 |
pobj |
mice,of |
| R955 |
T8991 |
T8992 |
dep |
that,urinated |
| R956 |
T9086 |
T9084 |
pobj |
substitution,to |
| R957 |
T9087 |
T9086 |
nmod |
valine,substitution |
| R958 |
T9088 |
T9087 |
prep |
for,valine |
| R959 |
T9089 |
T9088 |
pobj |
phenylalanine,for |
| R960 |
T9090 |
T9086 |
prep |
at,substitution |
| R961 |
T8992 |
T8988 |
relcl |
urinated,family |
| R962 |
T9091 |
T9092 |
compound |
amino,acid |
| R963 |
T9092 |
T9090 |
pobj |
acid,at |
| R964 |
T9093 |
T9092 |
nummod |
204,acid |
| R965 |
T9094 |
T9092 |
prep |
of,acid |
| R966 |
T8993 |
T8992 |
cc |
and,urinated |
| R967 |
T9095 |
T9096 |
det |
the,protein |
| R968 |
T9096 |
T9094 |
pobj |
protein,of |
| R969 |
T9097 |
T9092 |
punct |
(,acid |
| R970 |
T8994 |
T8992 |
conj |
drank,urinated |
| R971 |
T9098 |
T9092 |
appos |
F204V,acid |
| R972 |
T9099 |
T9092 |
punct |
),acid |
| R973 |
T9100 |
T9056 |
punct |
.,identified |
| R974 |
T8995 |
T8994 |
advmod |
excessively,drank |
| R975 |
T8996 |
T8952 |
punct |
.,found |
| R976 |
T9102 |
T9103 |
nsubj |
AQP2,is |
| R977 |
T9104 |
T9105 |
det |
a,channel |
| R978 |
T8998 |
T8999 |
nmod |
Serum,analysis |
| R979 |
T9105 |
T9103 |
attr |
channel,is |
| R980 |
T9106 |
T9107 |
nummod |
six,transmembrane |
| R981 |
T9107 |
T9105 |
compound |
transmembrane,channel |
| R982 |
T9108 |
T9107 |
punct |
-,transmembrane |
| R983 |
T8999 |
T9002 |
nsubj |
analysis,showed |
| R984 |
T9109 |
T9105 |
compound |
water,channel |
| R985 |
T9110 |
T9103 |
punct |
", ",is |
| R986 |
T9000 |
T8998 |
cc |
and,Serum |
| R987 |
T9111 |
T9103 |
cc |
and,is |
| R988 |
T9112 |
T9113 |
nsubj |
F204,lies |
| R989 |
T9113 |
T9103 |
conj |
lies,is |
| R990 |
T9001 |
T8998 |
conj |
urine,Serum |
| R991 |
T9114 |
T9113 |
prep |
near,lies |
| R992 |
T9115 |
T9116 |
det |
the,face |
| R993 |
T9003 |
T9004 |
mark |
that,were |
| R994 |
T9116 |
T9114 |
pobj |
face,near |
| R995 |
T9117 |
T9116 |
amod |
extracellular,face |
| R996 |
T9118 |
T9116 |
prep |
of,face |
| R997 |
T9119 |
T9120 |
det |
the,domain |
| R998 |
T9120 |
T9118 |
pobj |
domain,of |
| R999 |
T9004 |
T9002 |
ccomp |
were,showed |
| R1000 |
T9121 |
T9120 |
amod |
sixth,domain |
| R1001 |
T9005 |
T9006 |
compound |
plasma,levels |
| R1002 |
T9122 |
T9123 |
npadvmod |
membrane,spanning |
| R1003 |
T9123 |
T9120 |
amod |
spanning,domain |
| R1004 |
T9124 |
T9120 |
punct |
", ",domain |
| R1005 |
T9125 |
T9126 |
det |
a,region |
| R1006 |
T9006 |
T9004 |
nsubj |
levels,were |
| R1007 |
T9126 |
T9120 |
appos |
region,domain |
| R1008 |
T9127 |
T9126 |
amod |
rich,region |
| R1009 |
T9128 |
T9127 |
prep |
in,rich |
| R1010 |
T9129 |
T9130 |
amod |
hydrophobic,acids |
| R1011 |
T9130 |
T9128 |
pobj |
acids,in |
| R1012 |
T9131 |
T9130 |
compound |
amino,acids |
| R1013 |
T9007 |
T9006 |
compound |
glucose,levels |
| R1014 |
T9132 |
T9113 |
punct |
.,lies |
| R1015 |
T9134 |
T9135 |
nsubjpass |
This,conserved |
| R1016 |
T9008 |
T9004 |
acomp |
normal,were |
| R1017 |
T9136 |
T9134 |
cc |
and,This |
| R1018 |
T9137 |
T9138 |
det |
the,other |
| R1019 |
T9009 |
T9004 |
cc |
and,were |
| R1020 |
T9138 |
T9139 |
nmod |
other,domains |
| R1021 |
T9139 |
T9134 |
conj |
domains,This |
| R1022 |
T9140 |
T9141 |
npadvmod |
membrane,spanning |
| R1023 |
T9010 |
T9011 |
expl |
there,was |
| R1024 |
T9141 |
T9139 |
amod |
spanning,domains |
| R1025 |
T9142 |
T9141 |
punct |
-,spanning |
| R1026 |
T9011 |
T9004 |
conj |
was,were |
| R1027 |
T9143 |
T9135 |
auxpass |
are,conserved |
| R1028 |
T9144 |
T9135 |
prep |
among,conserved |
| R1029 |
T9145 |
T9146 |
amod |
vertebrate,species |
| R1030 |
T9012 |
T9013 |
det |
no,glucose |
| R1031 |
T9146 |
T9144 |
pobj |
species,among |
| R1032 |
T9147 |
T9135 |
punct |
.,conserved |
| R1033 |
T9013 |
T9011 |
attr |
glucose,was |
| R1034 |
T9149 |
T9150 |
det |
The,phenylalanine |
| R1035 |
T9150 |
T9151 |
nsubj |
phenylalanine,is |
| R1036 |
T9014 |
T9011 |
prep |
in,was |
| R1037 |
T9152 |
T9150 |
prep |
at,phenylalanine |
| R1038 |
T9015 |
T9016 |
det |
the,urine |
| R1039 |
T9016 |
T9014 |
pobj |
urine,in |
| R1040 |
T9153 |
T9152 |
pobj |
position,at |
| R1041 |
T9154 |
T9153 |
nummod |
204,position |
| R1042 |
T9017 |
T9018 |
punct |
(,data |
| R1043 |
T9155 |
T9156 |
advmod |
particularly,conserved |
| R1044 |
T9156 |
T9151 |
acomp |
conserved,is |
| R1045 |
T9018 |
T9002 |
meta |
data,showed |
| R1046 |
T9157 |
T9156 |
advmod |
well,conserved |
| R1047 |
T9158 |
T9159 |
punct |
(,Figure |
| R1048 |
T9159 |
T9156 |
parataxis |
Figure,conserved |
| R1049 |
T9019 |
T9018 |
amod |
unpublished,data |
| R1050 |
T9160 |
T9159 |
nummod |
1B,Figure |
| R1051 |
T9161 |
T9159 |
punct |
),Figure |
| R1052 |
T9020 |
T9018 |
punct |
),data |
| R1053 |
T9162 |
T9151 |
punct |
", ",is |
| R1054 |
T9163 |
T9164 |
preconj |
not,among |
| R1055 |
T9164 |
T9151 |
prep |
among,is |
| R1056 |
T9021 |
T9002 |
punct |
.,showed |
| R1057 |
T9165 |
T9163 |
advmod |
only,not |
| R1058 |
T9166 |
T9167 |
amod |
vertebrate,proteins |
| R1059 |
T9023 |
T9024 |
advmod |
Hence,was |
| R1060 |
T9167 |
T9164 |
pobj |
proteins,among |
| R1061 |
T9168 |
T9167 |
compound |
AQP2,proteins |
| R1062 |
T9169 |
T9164 |
punct |
", ",among |
| R1063 |
T9170 |
T9164 |
cc |
but,among |
| R1064 |
T9171 |
T9170 |
advmod |
also,but |
| R1065 |
T9172 |
T9164 |
conj |
among,among |
| R1066 |
T9173 |
T9174 |
compound |
others,members |
| R1067 |
T9025 |
T9024 |
punct |
", ",was |
| R1068 |
T9174 |
T9172 |
pobj |
members,among |
| R1069 |
T9175 |
T9174 |
prep |
of,members |
| R1070 |
T9176 |
T9177 |
det |
this,family |
| R1071 |
T9177 |
T9175 |
pobj |
family,of |
| R1072 |
T9178 |
T9151 |
punct |
.,is |
| R1073 |
T9026 |
T9024 |
nsubj |
this,was |
| R1074 |
T9180 |
T9181 |
compound |
Aqp2F204V,F204V |
| R1075 |
T9181 |
T9183 |
compound |
F204V,mice |
| R1076 |
T9027 |
T9028 |
det |
an,example |
| R1077 |
T9182 |
T9181 |
punct |
/,F204V |
| R1078 |
T9183 |
T9184 |
nsubj |
mice,have |
| R1079 |
T9028 |
T9024 |
attr |
example,was |
| R1080 |
T9185 |
T9186 |
advmod |
dramatically,increased |
| R1081 |
T9186 |
T9187 |
amod |
increased,production |
| R1082 |
T9029 |
T9028 |
prep |
of,example |
| R1083 |
T9187 |
T9184 |
dobj |
production,have |
| R1084 |
T9188 |
T9187 |
compound |
urine,production |
| R1085 |
T9189 |
T9184 |
punct |
", ",have |
| R1086 |
T9030 |
T9031 |
compound |
diabetes,insipidus |
| R1087 |
T9190 |
T9191 |
prep |
in,producing |
| R1088 |
T9191 |
T9184 |
advcl |
producing,have |
| R1089 |
T9031 |
T9029 |
pobj |
insipidus,of |
| R1090 |
T9032 |
T9024 |
punct |
.,was |
| R1091 |
T9034 |
T9035 |
det |
The,disorder |
| R1092 |
T9192 |
T9193 |
det |
some,cases |
| R1093 |
T9193 |
T9190 |
pobj |
cases,in |
| R1094 |
T9194 |
T9195 |
det |
an,amount |
| R1095 |
T9035 |
T9036 |
nsubj |
disorder,segregated |
| R1096 |
T9195 |
T9191 |
dobj |
amount,producing |
| R1097 |
T9196 |
T9195 |
prep |
of,amount |
| R1098 |
T9197 |
T9196 |
pobj |
urine,of |
| R1099 |
T9037 |
T9035 |
prep |
in,disorder |
| R1100 |
T9198 |
T9191 |
prep |
in,producing |
| R1101 |
T9199 |
T9200 |
nummod |
24,h |
| R1102 |
T9200 |
T9198 |
pobj |
h,in |
| R1103 |
T9201 |
T9202 |
dep |
that,exceeds |
| R1104 |
T9038 |
T9039 |
det |
these,mice |
| R1105 |
T9202 |
T9191 |
ccomp |
exceeds,producing |
| R1106 |
T9203 |
T9204 |
poss |
their,weight |
| R1107 |
T9204 |
T9202 |
dobj |
weight,exceeds |
| R1108 |
T9039 |
T9037 |
pobj |
mice,in |
| R1109 |
T9205 |
T9204 |
compound |
body,weight |
| R1110 |
T9206 |
T9191 |
punct |
", ",producing |
| R1111 |
T9040 |
T9036 |
prep |
in,segregated |
| R1112 |
T9207 |
T9191 |
prep |
compared,producing |
| R1113 |
T9208 |
T9207 |
prep |
to,compared |
| R1114 |
T9209 |
T9210 |
poss |
their,littermates |
| R1115 |
T9210 |
T9208 |
pobj |
littermates,to |
| R1116 |
T9211 |
T9210 |
amod |
heterozygous,littermates |
| R1117 |
T9212 |
T9211 |
cc |
or,heterozygous |
| R1118 |
T9041 |
T9042 |
det |
a,manner |
| R1119 |
T9213 |
T9214 |
amod |
wild,type |
| R1120 |
T9214 |
T9211 |
conj |
type,heterozygous |
| R1121 |
T9215 |
T9214 |
punct |
-,type |
| R1122 |
T9042 |
T9040 |
pobj |
manner,in |
| R1123 |
T9216 |
T9184 |
punct |
.,have |
| R1124 |
T9043 |
T9042 |
amod |
monogenic,manner |
| R1125 |
T9218 |
T9219 |
amod |
Such,loss |
| R1126 |
T9219 |
T9220 |
nsubj |
loss,lead |
| R1127 |
T9044 |
T9042 |
punct |
", ",manner |
| R1128 |
T9221 |
T9219 |
prep |
of,loss |
| R1129 |
T9222 |
T9221 |
pobj |
water,of |
| R1130 |
T9223 |
T9220 |
aux |
would,lead |
| R1131 |
T9224 |
T9220 |
advmod |
rapidly,lead |
| R1132 |
T9045 |
T9046 |
amod |
autosomal,recessive |
| R1133 |
T9225 |
T9220 |
prep |
to,lead |
| R1134 |
T9226 |
T9225 |
pobj |
dehydration,to |
| R1135 |
T9227 |
T9228 |
auxpass |
were,compensated |
| R1136 |
T9046 |
T9042 |
amod |
recessive,manner |
| R1137 |
T9228 |
T9220 |
advcl |
compensated,lead |
| R1138 |
T9229 |
T9228 |
nsubjpass |
it,compensated |
| R1139 |
T9047 |
T9036 |
punct |
", ",segregated |
| R1140 |
T9230 |
T9228 |
neg |
not,compensated |
| R1141 |
T9231 |
T9228 |
agent |
by,compensated |
| R1142 |
T9232 |
T9233 |
amod |
increased,intake |
| R1143 |
T9048 |
T9036 |
advcl |
making,segregated |
| R1144 |
T9233 |
T9231 |
pobj |
intake,by |
| R1145 |
T9234 |
T9233 |
compound |
water,intake |
| R1146 |
T9235 |
T9220 |
punct |
.,lead |
| R1147 |
T9049 |
T9050 |
nsubj |
Aqp2,gene |
| R1148 |
T9237 |
T9238 |
advmod |
Indeed,increase |
| R1149 |
T9050 |
T9048 |
ccomp |
gene,making |
| R1150 |
T9239 |
T9238 |
punct |
", ",increase |
| R1151 |
T9051 |
T9050 |
det |
a,gene |
| R1152 |
T9240 |
T9241 |
compound |
mutant,mice |
| R1153 |
T9052 |
T9050 |
compound |
candidate,gene |
| R1154 |
T9241 |
T9238 |
nsubj |
mice,increase |
| R1155 |
T9242 |
T9238 |
advmod |
also,increase |
| R1156 |
T9053 |
T9036 |
punct |
.,segregated |
| R1157 |
T9243 |
T9238 |
advmod |
dramatically,increase |
| R1158 |
T9244 |
T9245 |
poss |
their,intake |
| R1159 |
T9245 |
T9238 |
dobj |
intake,increase |
| R1160 |
T9055 |
T9056 |
nsubj |
Sequencing,identified |
| R1161 |
T9246 |
T9245 |
compound |
water,intake |
| R1162 |
T9247 |
T9248 |
punct |
(,Figure |
| R1163 |
T9248 |
T9238 |
parataxis |
Figure,increase |
| R1164 |
T9249 |
T9248 |
nummod |
1C,Figure |
| R1165 |
T9057 |
T9055 |
prep |
of,Sequencing |
| R1166 |
T9250 |
T9248 |
punct |
),Figure |
| R1167 |
T9251 |
T9238 |
prep |
compared,increase |
| R1168 |
T9252 |
T9251 |
prep |
to,compared |
| R1169 |
T9253 |
T9254 |
poss |
their,littermates |
| R1170 |
T9254 |
T9252 |
pobj |
littermates,to |
| R1171 |
T9058 |
T9059 |
compound |
Aqp2,region |
| R1172 |
T9255 |
T9254 |
amod |
heterozygous,littermates |
| R1173 |
T9256 |
T9255 |
cc |
or,heterozygous |
| R1174 |
T9257 |
T9258 |
amod |
wild,type |
| R1175 |
T9059 |
T9057 |
pobj |
region,of |
| R1176 |
T9258 |
T9255 |
conj |
type,heterozygous |
| R1177 |
T9259 |
T9258 |
punct |
-,type |
| R1178 |
T9260 |
T9238 |
punct |
.,increase |
| R1179 |
T9060 |
T9059 |
compound |
coding,region |
| R1180 |
T9262 |
T9263 |
det |
This,phenotype |
| R1181 |
T9061 |
T9059 |
prep |
of,region |
| R1182 |
T9263 |
T9264 |
nsubj |
phenotype,showed |
| R1183 |
T9265 |
T9263 |
punct |
—,phenotype |
| R1184 |
T9062 |
T9063 |
amod |
affected,mice |
| R1185 |
T9266 |
T9267 |
amod |
increased,output |
| R1186 |
T9267 |
T9263 |
appos |
output,phenotype |
| R1187 |
T9268 |
T9267 |
amod |
urinary,output |
| R1188 |
T9063 |
T9061 |
pobj |
mice,of |
| R1189 |
T9269 |
T9267 |
cc |
and,output |
| R1190 |
T9270 |
T9271 |
compound |
water,intake |
| R1191 |
T9271 |
T9267 |
conj |
intake,output |
| R1192 |
T9064 |
T9065 |
det |
a,transversion |
| R1193 |
T9272 |
T9264 |
punct |
—,showed |
| R1194 |
T9273 |
T9274 |
amod |
complete,concordance |
| R1195 |
T9274 |
T9264 |
dobj |
concordance,showed |
| R1196 |
T9065 |
T9056 |
dobj |
transversion,identified |
| R1197 |
T9275 |
T9274 |
prep |
with,concordance |
| R1198 |
T9276 |
T9275 |
pobj |
homozygosity,with |
| R1199 |
T9277 |
T9276 |
prep |
of,homozygosity |
| R1200 |
T9066 |
T9065 |
nmod |
thymine,transversion |
| R1201 |
T9278 |
T9279 |
det |
the,mutation |
| R1202 |
T9279 |
T9277 |
pobj |
mutation,of |
| R1203 |
T9280 |
T9279 |
compound |
F204V,mutation |
| R1204 |
T9067 |
T9066 |
prep |
to,thymine |
| R1205 |
T9281 |
T9264 |
prep |
in,showed |
| R1206 |
T9282 |
T9283 |
det |
the,animals |
| R1207 |
T9283 |
T9281 |
pobj |
animals,in |
| R1208 |
T9068 |
T9067 |
pobj |
guanine,to |
| R1209 |
T9284 |
T9283 |
nummod |
58,animals |
| R1210 |
T9285 |
T9283 |
acl |
tested,animals |
| R1211 |
T9286 |
T9264 |
punct |
.,showed |
| R1212 |
T9069 |
T9066 |
punct |
(,thymine |
| R1213 |
T9288 |
T9289 |
compound |
Diabetes,insipidus |
| R1214 |
T9289 |
T9290 |
nsubjpass |
insipidus,defined |
| R1215 |
T9070 |
T9066 |
appos |
T,thymine |
| R1216 |
T9291 |
T9290 |
aux |
can,defined |
| R1217 |
T9292 |
T9290 |
auxpass |
be,defined |
| R1218 |
T9293 |
T9290 |
prep |
as,defined |
| R1219 |
T9294 |
T9295 |
det |
an,inability |
| R1220 |
T9295 |
T9293 |
pobj |
inability,as |
| R1221 |
T9071 |
T9070 |
prep |
to,T |
| R1222 |
T9296 |
T9297 |
aux |
to,concentrate |
| R1223 |
T9297 |
T9295 |
acl |
concentrate,inability |
| R1224 |
T9072 |
T9071 |
pobj |
G,to |
| R1225 |
T9073 |
T9065 |
punct |
),transversion |
| R1226 |
T9074 |
T9075 |
punct |
(,Figure |
| R1227 |
T9298 |
T9297 |
dobj |
urine,concentrate |
| R1228 |
T9299 |
T9300 |
advmod |
where,appropriate |
| R1229 |
T9075 |
T9065 |
parataxis |
Figure,transversion |
| R1230 |
T9300 |
T9297 |
advcl |
appropriate,concentrate |
| R1231 |
T9301 |
T9290 |
punct |
.,defined |
| R1232 |
T9076 |
T9075 |
nummod |
1A,Figure |
| R1233 |
T9303 |
T9304 |
prep |
Compared,produce |
| R1234 |
T9305 |
T9303 |
prep |
to,Compared |
| R1235 |
T9077 |
T9075 |
punct |
),Figure |
| R1236 |
T9306 |
T9307 |
amod |
wild,type |
| R1237 |
T9307 |
T9309 |
nmod |
type,littermates |
| R1238 |
T9308 |
T9307 |
punct |
-,type |
| R1239 |
T9078 |
T9065 |
punct |
", ",transversion |
| R1240 |
T9309 |
T9305 |
pobj |
littermates,to |
| R1241 |
T9310 |
T9307 |
cc |
or,type |
| R1242 |
T9311 |
T9307 |
conj |
heterozygous,type |
| R1243 |
T9079 |
T9080 |
dep |
which,predicted |
| R1244 |
T9312 |
T9304 |
punct |
", ",produce |
| R1245 |
T9313 |
T9314 |
compound |
Aqp2F204V,F204V |
| R1246 |
T9314 |
T9316 |
compound |
F204V,mice |
| R1247 |
T9080 |
T9065 |
relcl |
predicted,transversion |
| R1248 |
T9315 |
T9314 |
punct |
/,F204V |
| R1249 |
T9316 |
T9304 |
nsubj |
mice,produce |
| R1250 |
T9317 |
T9318 |
advmod |
very,dilute |
| R1251 |
T9318 |
T9319 |
amod |
dilute,urine |
| R1252 |
T9081 |
T9080 |
auxpass |
is,predicted |
| R1253 |
T9319 |
T9304 |
dobj |
urine,produce |
| R1254 |
T9320 |
T9321 |
punct |
(,Figure |
| R1255 |
T9321 |
T9304 |
parataxis |
Figure,produce |
| R1256 |
T9082 |
T9083 |
aux |
to,lead |
| R1257 |
T9322 |
T9321 |
nummod |
1D,Figure |
| R1258 |
T9323 |
T9321 |
punct |
),Figure |
| R1259 |
T9083 |
T9080 |
xcomp |
lead,predicted |
| R1260 |
T9324 |
T9304 |
punct |
.,produce |
| R1261 |
T9326 |
T9327 |
amod |
Basal,concentration |
| R1262 |
T9084 |
T9083 |
prep |
to,lead |
| R1263 |
T9085 |
T9086 |
det |
a,substitution |
| R1264 |
T9327 |
T9329 |
nsubj |
concentration,is |
| R1265 |
T9403 |
T9402 |
oprd |
desmopressin,called |
| R1266 |
T9328 |
T9327 |
compound |
urine,concentration |
| R1267 |
T9330 |
T9327 |
prep |
in,concentration |
| R1268 |
T9331 |
T9332 |
compound |
mutant,mice |
| R1269 |
T9332 |
T9330 |
pobj |
mice,in |
| R1270 |
T9333 |
T9334 |
advmod |
about,161 |
| R1271 |
T9404 |
T9383 |
punct |
),analog |
| R1272 |
T9334 |
T9335 |
nummod |
161,mOsm |
| R1273 |
T9335 |
T9329 |
attr |
mOsm,is |
| R1274 |
T9405 |
T9386 |
punct |
", ",is |
| R1275 |
T9336 |
T9335 |
punct |
", ",mOsm |
| R1276 |
T9337 |
T9335 |
prep |
compared,mOsm |
| R1277 |
T9338 |
T9337 |
prep |
to,compared |
| R1278 |
T9406 |
T9407 |
det |
a,agonist |
| R1279 |
T9339 |
T9340 |
quantmod |
about,"1,293" |
| R1280 |
T9340 |
T9341 |
nummod |
"1,293",mOsm |
| R1281 |
T9341 |
T9338 |
pobj |
mOsm,to |
| R1282 |
T9407 |
T9386 |
attr |
agonist,is |
| R1283 |
T9342 |
T9341 |
prep |
in,mOsm |
| R1284 |
T9343 |
T9344 |
amod |
wild,type |
| R1285 |
T9344 |
T9346 |
compound |
type,mice |
| R1286 |
T9408 |
T9407 |
amod |
potent,agonist |
| R1287 |
T9345 |
T9344 |
punct |
-,type |
| R1288 |
T9346 |
T9342 |
pobj |
mice,in |
| R1289 |
T9409 |
T9407 |
prep |
of,agonist |
| R1290 |
T9347 |
T9348 |
punct |
(,0.001 |
| R1291 |
T9348 |
T9329 |
parataxis |
0.001,is |
| R1292 |
T9349 |
T9348 |
nsubj |
p,0.001 |
| R1293 |
T9410 |
T9409 |
pobj |
AVPR2,of |
| R1294 |
T9350 |
T9348 |
punct |
<,0.001 |
| R1295 |
T9351 |
T9348 |
punct |
),0.001 |
| R1296 |
T9352 |
T9329 |
punct |
.,is |
| R1297 |
T9411 |
T9386 |
punct |
.,is |
| R1298 |
T9354 |
T9355 |
advmod |
Normally,is |
| R1299 |
T9413 |
T9414 |
advmod |
When,administered |
| R1300 |
T9356 |
T9355 |
punct |
", ",is |
| R1301 |
T9357 |
T9358 |
compound |
urine,concentration |
| R1302 |
T9358 |
T9355 |
nsubj |
concentration,is |
| R1303 |
T9359 |
T9355 |
prep |
under,is |
| R1304 |
T9360 |
T9361 |
det |
the,control |
| R1305 |
T9361 |
T9359 |
pobj |
control,under |
| R1306 |
T9414 |
T9415 |
advcl |
administered,leads |
| R1307 |
T9362 |
T9361 |
prep |
of,control |
| R1308 |
T9363 |
T9364 |
det |
the,hypothalamus |
| R1309 |
T9364 |
T9362 |
pobj |
hypothalamus,of |
| R1310 |
T9365 |
T9364 |
punct |
", ",hypothalamus |
| R1311 |
T9416 |
T9414 |
prep |
to,administered |
| R1312 |
T9366 |
T9367 |
dep |
which,secretes |
| R1313 |
T9367 |
T9364 |
relcl |
secretes,hypothalamus |
| R1314 |
T9368 |
T9367 |
punct |
", ",secretes |
| R1315 |
T9369 |
T9367 |
prep |
in,secretes |
| R1316 |
T9417 |
T9418 |
amod |
wild,type |
| R1317 |
T9370 |
T9369 |
pobj |
response,in |
| R1318 |
T9371 |
T9370 |
prep |
to,response |
| R1319 |
T9372 |
T9371 |
pobj |
hypovolemia,to |
| R1320 |
T9373 |
T9372 |
cc |
or,hypovolemia |
| R1321 |
T9374 |
T9372 |
conj |
hypernatremia,hypovolemia |
| R1322 |
T9375 |
T9376 |
punct |
[,21 |
| R1323 |
T9418 |
T9420 |
compound |
type,mice |
| R1324 |
T9376 |
T9370 |
parataxis |
21,response |
| R1325 |
T9377 |
T9376 |
punct |
],21 |
| R1326 |
T9378 |
T9367 |
punct |
", ",secretes |
| R1327 |
T9379 |
T9367 |
dobj |
AVP,secretes |
| R1328 |
T9419 |
T9418 |
punct |
-,type |
| R1329 |
T9380 |
T9355 |
punct |
.,is |
| R1330 |
T9382 |
T9383 |
det |
The,analog |
| R1331 |
T9420 |
T9416 |
pobj |
mice,to |
| R1332 |
T9383 |
T9386 |
nsubj |
analog,is |
| R1333 |
T9384 |
T9383 |
amod |
synthetic,analog |
| R1334 |
T9421 |
T9415 |
punct |
", ",leads |
| R1335 |
T9385 |
T9383 |
compound |
AVP,analog |
| R1336 |
T9387 |
T9383 |
punct |
", ",analog |
| R1337 |
T9422 |
T9415 |
nsubj |
dDAVP,leads |
| R1338 |
T9388 |
T9389 |
nummod |
1,deamino |
| R1339 |
T9389 |
T9391 |
nmod |
deamino,arginine |
| R1340 |
T9390 |
T9389 |
punct |
-,deamino |
| R1341 |
T9423 |
T9415 |
prep |
to,leads |
| R1342 |
T9424 |
T9425 |
det |
a,increase |
| R1343 |
T9391 |
T9397 |
compound |
arginine,vasopressin |
| R1344 |
T9425 |
T9423 |
pobj |
increase,to |
| R1345 |
T9392 |
T9391 |
punct |
-,arginine |
| R1346 |
T9393 |
T9391 |
nummod |
8,arginine |
| R1347 |
T9426 |
T9425 |
amod |
dramatic,increase |
| R1348 |
T9394 |
T9391 |
punct |
-,arginine |
| R1349 |
T9395 |
T9391 |
compound |
D,arginine |
| R1350 |
T9396 |
T9391 |
punct |
-,arginine |
| R1351 |
T9427 |
T9425 |
prep |
in,increase |
| R1352 |
T9397 |
T9383 |
appos |
vasopressin,analog |
| R1353 |
T9398 |
T9397 |
punct |
(,vasopressin |
| R1354 |
T9399 |
T9397 |
appos |
dDAVP,vasopressin |
| R1355 |
T9428 |
T9429 |
compound |
urine,concentration |
| R1356 |
T9400 |
T9397 |
punct |
;,vasopressin |
| R1357 |
T9401 |
T9402 |
advmod |
also,called |
| R1358 |
T9402 |
T9397 |
acl |
called,vasopressin |
| R1359 |
T9429 |
T9427 |
pobj |
concentration,in |
| R1360 |
T9430 |
T9425 |
punct |
", ",increase |
| R1361 |
T9431 |
T9425 |
prep |
from,increase |
| R1362 |
T9432 |
T9431 |
pobj |
"1,293",from |
| R1363 |
T9509 |
T9510 |
dep |
which,be |
| R1364 |
T9510 |
T9505 |
relcl |
be,channel |
| R1365 |
T9433 |
T9431 |
prep |
to,from |
| R1366 |
T9511 |
T9510 |
aux |
must,be |
| R1367 |
T9512 |
T9510 |
acomp |
sufficient,be |
| R1368 |
T9513 |
T9514 |
aux |
to,allow |
| R1369 |
T9434 |
T9433 |
pobj |
"5,885",to |
| R1370 |
T9514 |
T9512 |
xcomp |
allow,sufficient |
| R1371 |
T9515 |
T9514 |
dobj |
survival,allow |
| R1372 |
T9516 |
T9515 |
prep |
of,survival |
| R1373 |
T9517 |
T9518 |
det |
the,individual |
| R1374 |
T9518 |
T9516 |
pobj |
individual,of |
| R1375 |
T9519 |
T9497 |
punct |
", ",indicates |
| R1376 |
T9435 |
T9431 |
pobj |
mOsm,from |
| R1377 |
T9520 |
T9497 |
prep |
in,indicates |
| R1378 |
T9521 |
T9520 |
pobj |
contrast,in |
| R1379 |
T9522 |
T9521 |
prep |
to,contrast |
| R1380 |
T9436 |
T9437 |
punct |
(,Figure |
| R1381 |
T9523 |
T9524 |
det |
the,mouse |
| R1382 |
T9524 |
T9522 |
pobj |
mouse,to |
| R1383 |
T9437 |
T9415 |
parataxis |
Figure,leads |
| R1384 |
T9525 |
T9524 |
nmod |
T126M,mouse |
| R1385 |
T9526 |
T9524 |
amod |
knock,mouse |
| R1386 |
T9527 |
T9526 |
punct |
-,knock |
| R1387 |
T9528 |
T9526 |
prt |
in,knock |
| R1388 |
T9438 |
T9437 |
advmod |
4.6-fold,Figure |
| R1389 |
T9529 |
T9530 |
punct |
[,18 |
| R1390 |
T9530 |
T9497 |
parataxis |
18,indicates |
| R1391 |
T9531 |
T9530 |
punct |
],18 |
| R1392 |
T9439 |
T9437 |
punct |
;,Figure |
| R1393 |
T9532 |
T9497 |
punct |
.,indicates |
| R1394 |
T9534 |
T9535 |
amod |
Multiple,matings |
| R1395 |
T9440 |
T9437 |
nummod |
1D,Figure |
| R1396 |
T9535 |
T9537 |
nsubj |
matings,yielded |
| R1397 |
T9536 |
T9535 |
amod |
heterozygous,matings |
| R1398 |
T9441 |
T9437 |
punct |
),Figure |
| R1399 |
T9538 |
T9539 |
nummod |
101,animals |
| R1400 |
T9539 |
T9537 |
dobj |
animals,yielded |
| R1401 |
T9442 |
T9415 |
punct |
.,leads |
| R1402 |
T9540 |
T9539 |
punct |
", ",animals |
| R1403 |
T9541 |
T9542 |
dep |
which,appeared |
| R1404 |
T9542 |
T9539 |
relcl |
appeared,animals |
| R1405 |
T9543 |
T9542 |
prep |
at,appeared |
| R1406 |
T9444 |
T9445 |
prep |
With,concentrate |
| R1407 |
T9544 |
T9545 |
det |
a,ratio |
| R1408 |
T9545 |
T9543 |
pobj |
ratio,at |
| R1409 |
T9546 |
T9545 |
prep |
of,ratio |
| R1410 |
T9547 |
T9546 |
pobj |
26,of |
| R1411 |
T9446 |
T9447 |
amod |
similar,treatment |
| R1412 |
T9548 |
T9549 |
punct |
:,49 |
| R1413 |
T9549 |
T9547 |
prep |
49,26 |
| R1414 |
T9550 |
T9551 |
punct |
:,26 |
| R1415 |
T9447 |
T9444 |
pobj |
treatment,With |
| R1416 |
T9551 |
T9547 |
prep |
26,26 |
| R1417 |
T9552 |
T9542 |
punct |
", ",appeared |
| R1418 |
T9553 |
T9542 |
prep |
near,appeared |
| R1419 |
T9448 |
T9445 |
punct |
", ",concentrate |
| R1420 |
T9554 |
T9555 |
det |
the,frequencies |
| R1421 |
T9555 |
T9553 |
pobj |
frequencies,near |
| R1422 |
T9556 |
T9555 |
amod |
expected,frequencies |
| R1423 |
T9557 |
T9558 |
amod |
Mendelian,type |
| R1424 |
T9558 |
T9555 |
nmod |
type,frequencies |
| R1425 |
T9559 |
T9558 |
amod |
wild,type |
| R1426 |
T9449 |
T9450 |
compound |
mutant,mice |
| R1427 |
T9560 |
T9558 |
punct |
", ",type |
| R1428 |
T9561 |
T9558 |
conj |
heterozygote,type |
| R1429 |
T9562 |
T9561 |
punct |
", ",heterozygote |
| R1430 |
T9450 |
T9445 |
nsubj |
mice,concentrate |
| R1431 |
T9563 |
T9561 |
cc |
and,heterozygote |
| R1432 |
T9564 |
T9561 |
conj |
mutant,heterozygote |
| R1433 |
T9565 |
T9542 |
punct |
", ",appeared |
| R1434 |
T9451 |
T9452 |
poss |
their,urine |
| R1435 |
T9566 |
T9542 |
advmod |
respectively,appeared |
| R1436 |
T9567 |
T9537 |
punct |
", ",yielded |
| R1437 |
T9452 |
T9445 |
dobj |
urine,concentrate |
| R1438 |
T9568 |
T9537 |
advcl |
indicating,yielded |
| R1439 |
T9569 |
T9570 |
mark |
that,is |
| R1440 |
T9570 |
T9568 |
ccomp |
is,indicating |
| R1441 |
T9571 |
T9570 |
expl |
there,is |
| R1442 |
T9453 |
T9445 |
prep |
to,concentrate |
| R1443 |
T9572 |
T9573 |
det |
no,viability |
| R1444 |
T9573 |
T9570 |
attr |
viability,is |
| R1445 |
T9454 |
T9455 |
det |
a,extent |
| R1446 |
T9574 |
T9573 |
amod |
reduced,viability |
| R1447 |
T9575 |
T9573 |
acl |
associated,viability |
| R1448 |
T9576 |
T9575 |
prep |
with,associated |
| R1449 |
T9455 |
T9453 |
pobj |
extent,to |
| R1450 |
T9577 |
T9578 |
det |
this,mutation |
| R1451 |
T9578 |
T9576 |
pobj |
mutation,with |
| R1452 |
T9579 |
T9537 |
punct |
.,yielded |
| R1453 |
T9456 |
T9455 |
amod |
lesser,extent |
| R1454 |
T9581 |
T9582 |
amod |
Other,was |
| R1455 |
T9457 |
T9456 |
cc |
but,lesser |
| R1456 |
T9583 |
T9581 |
prep |
than,Other |
| R1457 |
T9458 |
T9459 |
advmod |
still,significant |
| R1458 |
T9459 |
T9456 |
conj |
significant,lesser |
| R1459 |
T9584 |
T9585 |
det |
the,production |
| R1460 |
T9585 |
T9583 |
pobj |
production,than |
| R1461 |
T9586 |
T9585 |
amod |
increased,production |
| R1462 |
T9460 |
T9445 |
punct |
", ",concentrate |
| R1463 |
T9587 |
T9585 |
compound |
urine,production |
| R1464 |
T9588 |
T9585 |
cc |
and,production |
| R1465 |
T9589 |
T9590 |
compound |
water,intake |
| R1466 |
T9461 |
T9445 |
prep |
from,concentrate |
| R1467 |
T9590 |
T9585 |
conj |
intake,production |
| R1468 |
T9591 |
T9582 |
punct |
", ",was |
| R1469 |
T9592 |
T9582 |
expl |
there,was |
| R1470 |
T9462 |
T9461 |
pobj |
161,from |
| R1471 |
T9593 |
T9594 |
det |
no,phenotype |
| R1472 |
T9594 |
T9582 |
attr |
phenotype,was |
| R1473 |
T9463 |
T9461 |
prep |
to,from |
| R1474 |
T9595 |
T9594 |
amod |
overt,phenotype |
| R1475 |
T9596 |
T9594 |
prep |
in,phenotype |
| R1476 |
T9597 |
T9598 |
compound |
mutant,mice |
| R1477 |
T9598 |
T9596 |
pobj |
mice,in |
| R1478 |
T9599 |
T9594 |
punct |
", ",phenotype |
| R1479 |
T9600 |
T9594 |
prep |
save,phenotype |
| R1480 |
T9464 |
T9463 |
pobj |
470,to |
| R1481 |
T9601 |
T9602 |
amod |
distended,kidneys |
| R1482 |
T9602 |
T9600 |
pobj |
kidneys,save |
| R1483 |
T9603 |
T9602 |
punct |
", ",kidneys |
| R1484 |
T9465 |
T9461 |
pobj |
mOsm,from |
| R1485 |
T9604 |
T9605 |
dep |
which,appeared |
| R1486 |
T9605 |
T9602 |
relcl |
appeared,kidneys |
| R1487 |
T9606 |
T9605 |
advmod |
variably,appeared |
| R1488 |
T9466 |
T9467 |
punct |
(,2.9-fold |
| R1489 |
T9607 |
T9605 |
prep |
in,appeared |
| R1490 |
T9608 |
T9609 |
amod |
adult,animals |
| R1491 |
T9609 |
T9607 |
pobj |
animals,in |
| R1492 |
T9467 |
T9461 |
parataxis |
2.9-fold,from |
| R1493 |
T9610 |
T9611 |
punct |
(,Figure |
| R1494 |
T9611 |
T9582 |
parataxis |
Figure,was |
| R1495 |
T9612 |
T9611 |
nummod |
2A,Figure |
| R1496 |
T9468 |
T9467 |
punct |
),2.9-fold |
| R1497 |
T9613 |
T9611 |
punct |
),Figure |
| R1498 |
T9614 |
T9582 |
punct |
.,was |
| R1499 |
T9469 |
T9445 |
punct |
", ",concentrate |
| R1500 |
T9470 |
T9445 |
advcl |
indicating,concentrate |
| R1501 |
T9471 |
T9472 |
mark |
that,are |
| R1502 |
T9616 |
T9617 |
mark |
Although,measured |
| R1503 |
T9472 |
T9470 |
ccomp |
are,indicating |
| R1504 |
T9617 |
T9620 |
advcl |
measured,seem |
| R1505 |
T9618 |
T9617 |
neg |
not,measured |
| R1506 |
T9619 |
T9617 |
advmod |
specifically,measured |
| R1507 |
T9473 |
T9474 |
det |
these,animals |
| R1508 |
T9621 |
T9620 |
punct |
", ",seem |
| R1509 |
T9622 |
T9623 |
compound |
mutant,mice |
| R1510 |
T9474 |
T9472 |
nsubj |
animals,are |
| R1511 |
T9623 |
T9620 |
nsubj |
mice,seem |
| R1512 |
T9624 |
T9625 |
aux |
to,have |
| R1513 |
T9625 |
T9620 |
xcomp |
have,seem |
| R1514 |
T9475 |
T9472 |
preconj |
not,are |
| R1515 |
T9626 |
T9627 |
det |
a,lifespan |
| R1516 |
T9627 |
T9625 |
dobj |
lifespan,have |
| R1517 |
T9628 |
T9627 |
amod |
normal,lifespan |
| R1518 |
T9476 |
T9475 |
advmod |
only,not |
| R1519 |
T9629 |
T9620 |
punct |
.,seem |
| R1520 |
T9477 |
T9472 |
conj |
unable,are |
| R1521 |
T9631 |
T9632 |
det |
The,animal |
| R1522 |
T9632 |
T9634 |
nsubj |
animal,lived |
| R1523 |
T9633 |
T9632 |
nummod |
one,animal |
| R1524 |
T9478 |
T9479 |
aux |
to,concentrate |
| R1525 |
T9635 |
T9636 |
dep |
that,followed |
| R1526 |
T9636 |
T9632 |
relcl |
followed,animal |
| R1527 |
T9637 |
T9636 |
auxpass |
was,followed |
| R1528 |
T9638 |
T9634 |
prep |
to,lived |
| R1529 |
T9639 |
T9640 |
nummod |
18,mo |
| R1530 |
T9479 |
T9477 |
xcomp |
concentrate,unable |
| R1531 |
T9640 |
T9638 |
pobj |
mo,to |
| R1532 |
T9641 |
T9634 |
punct |
", ",lived |
| R1533 |
T9642 |
T9634 |
advcl |
typical,lived |
| R1534 |
T9480 |
T9481 |
poss |
their,urine |
| R1535 |
T9643 |
T9642 |
prep |
for,typical |
| R1536 |
T9644 |
T9643 |
pobj |
animals,for |
| R1537 |
T9645 |
T9644 |
prep |
in,animals |
| R1538 |
T9481 |
T9479 |
dobj |
urine,concentrate |
| R1539 |
T9646 |
T9647 |
poss |
our,colony |
| R1540 |
T9647 |
T9645 |
pobj |
colony,in |
| R1541 |
T9648 |
T9634 |
punct |
.,lived |
| R1542 |
T9482 |
T9479 |
advmod |
properly,concentrate |
| R1543 |
T9650 |
T9651 |
compound |
Aqp2F204V,F204V |
| R1544 |
T9651 |
T9653 |
compound |
F204V,mice |
| R1545 |
T9483 |
T9472 |
cc |
but,are |
| R1546 |
T9652 |
T9651 |
punct |
/,F204V |
| R1547 |
T9653 |
T9654 |
nsubj |
mice,suffer |
| R1548 |
T9484 |
T9472 |
conj |
are,are |
| R1549 |
T9655 |
T9654 |
prep |
from,suffer |
| R1550 |
T9656 |
T9657 |
amod |
severe,hydronephrosis |
| R1551 |
T9485 |
T9484 |
advmod |
also,are |
| R1552 |
T9657 |
T9655 |
pobj |
hydronephrosis,from |
| R1553 |
T9658 |
T9659 |
punct |
(,2A |
| R1554 |
T9486 |
T9484 |
acomp |
defective,are |
| R1555 |
T9659 |
T9654 |
parataxis |
2A,suffer |
| R1556 |
T9660 |
T9659 |
nmod |
Figure,2A |
| R1557 |
T9661 |
T9659 |
cc |
and,2A |
| R1558 |
T9487 |
T9484 |
prep |
in,are |
| R1559 |
T9662 |
T9659 |
conj |
2B,2A |
| R1560 |
T9663 |
T9659 |
punct |
),2A |
| R1561 |
T9664 |
T9654 |
punct |
", ",suffer |
| R1562 |
T9488 |
T9489 |
poss |
their,response |
| R1563 |
T9665 |
T9666 |
advmod |
presumably,as |
| R1564 |
T9666 |
T9654 |
prep |
as,suffer |
| R1565 |
T9667 |
T9668 |
det |
a,consequence |
| R1566 |
T9489 |
T9487 |
pobj |
response,in |
| R1567 |
T9668 |
T9666 |
pobj |
consequence,as |
| R1568 |
T9669 |
T9668 |
prep |
of,consequence |
| R1569 |
T9490 |
T9489 |
prep |
to,response |
| R1570 |
T9491 |
T9490 |
pobj |
dDAVP,to |
| R1571 |
T9670 |
T9671 |
det |
an,inability |
| R1572 |
T9492 |
T9445 |
punct |
.,concentrate |
| R1573 |
T9671 |
T9669 |
pobj |
inability,of |
| R1574 |
T9672 |
T9673 |
aux |
to,cope |
| R1575 |
T9673 |
T9671 |
acl |
cope,inability |
| R1576 |
T9494 |
T9495 |
det |
The,response |
| R1577 |
T9674 |
T9673 |
prep |
with,cope |
| R1578 |
T9675 |
T9676 |
det |
the,polyuria |
| R1579 |
T9676 |
T9674 |
pobj |
polyuria,with |
| R1580 |
T9677 |
T9676 |
amod |
extreme,polyuria |
| R1581 |
T9678 |
T9654 |
punct |
.,suffer |
| R1582 |
T9495 |
T9497 |
nsubj |
response,indicates |
| R1583 |
T9680 |
T9681 |
nsubj |
We,found |
| R1584 |
T9681 |
T9682 |
ccomp |
found,was |
| R1585 |
T9496 |
T9495 |
amod |
smaller,response |
| R1586 |
T9683 |
T9684 |
amod |
distended,kidneys |
| R1587 |
T9684 |
T9681 |
dobj |
kidneys,found |
| R1588 |
T9685 |
T9681 |
prep |
in,found |
| R1589 |
T9498 |
T9495 |
prep |
to,response |
| R1590 |
T9686 |
T9687 |
det |
all,mice |
| R1591 |
T9687 |
T9685 |
pobj |
mice,in |
| R1592 |
T9688 |
T9689 |
compound |
Aqp2F204V,F204V |
| R1593 |
T9689 |
T9687 |
compound |
F204V,mice |
| R1594 |
T9499 |
T9498 |
pobj |
dDAVP,to |
| R1595 |
T9690 |
T9689 |
punct |
/,F204V |
| R1596 |
T9691 |
T9682 |
punct |
;,was |
| R1597 |
T9692 |
T9682 |
advmod |
however,was |
| R1598 |
T9500 |
T9501 |
det |
some,activity |
| R1599 |
T9693 |
T9682 |
punct |
", ",was |
| R1600 |
T9694 |
T9695 |
det |
the,degree |
| R1601 |
T9695 |
T9682 |
nsubj |
degree,was |
| R1602 |
T9501 |
T9497 |
dobj |
activity,indicates |
| R1603 |
T9696 |
T9695 |
prep |
of,degree |
| R1604 |
T9697 |
T9696 |
pobj |
inflation,of |
| R1605 |
T9502 |
T9501 |
amod |
residual,activity |
| R1606 |
T9698 |
T9682 |
acomp |
variable,was |
| R1607 |
T9699 |
T9682 |
prep |
in,was |
| R1608 |
T9700 |
T9701 |
amod |
affected,mice |
| R1609 |
T9503 |
T9501 |
prep |
of,activity |
| R1610 |
T9701 |
T9699 |
pobj |
mice,in |
| R1611 |
T9702 |
T9682 |
cc |
and,was |
| R1612 |
T9703 |
T9682 |
conj |
worsened,was |
| R1613 |
T9704 |
T9703 |
prep |
with,worsened |
| R1614 |
T9504 |
T9505 |
det |
the,channel |
| R1615 |
T9705 |
T9704 |
pobj |
age,with |
| R1616 |
T9706 |
T9682 |
punct |
.,was |
| R1617 |
T9505 |
T9503 |
pobj |
channel,of |
| R1618 |
T9708 |
T9709 |
amod |
Severe,hydronephrosis |
| R1619 |
T9709 |
T9710 |
nsubjpass |
hydronephrosis,observed |
| R1620 |
T9506 |
T9505 |
compound |
mutant,channel |
| R1621 |
T9711 |
T9710 |
aux |
has,observed |
| R1622 |
T9712 |
T9710 |
advmod |
previously,observed |
| R1623 |
T9507 |
T9505 |
compound |
AQP2,channel |
| R1624 |
T9713 |
T9710 |
auxpass |
been,observed |
| R1625 |
T9714 |
T9710 |
prep |
in,observed |
| R1626 |
T9715 |
T9716 |
amod |
double,knock |
| R1627 |
T9508 |
T9505 |
punct |
", ",channel |
| R1628 |
T9716 |
T9720 |
amod |
knock,mice |
| R1629 |
T9717 |
T9718 |
compound |
Aqp1,Aqp3 |
| R1630 |
T9718 |
T9716 |
npadvmod |
Aqp3,knock |
| R1631 |
T9719 |
T9718 |
punct |
/,Aqp3 |
| R1632 |
T9720 |
T9714 |
pobj |
mice,in |
| R1633 |
T9827 |
T9828 |
det |
the,features |
| R1634 |
T9721 |
T9716 |
punct |
-,knock |
| R1635 |
T9828 |
T9825 |
nsubj |
features,were |
| R1636 |
T9722 |
T9716 |
prt |
out,knock |
| R1637 |
T9723 |
T9724 |
punct |
[,17 |
| R1638 |
T9829 |
T9828 |
amod |
morphologic,features |
| R1639 |
T9724 |
T9710 |
parataxis |
17,observed |
| R1640 |
T9725 |
T9724 |
punct |
],17 |
| R1641 |
T9726 |
T9710 |
punct |
", ",observed |
| R1642 |
T9830 |
T9828 |
prep |
of,features |
| R1643 |
T9727 |
T9710 |
cc |
and,observed |
| R1644 |
T9728 |
T9710 |
conj |
appears,observed |
| R1645 |
T9729 |
T9728 |
prep |
at,appears |
| R1646 |
T9831 |
T9832 |
det |
the,glomeruli |
| R1647 |
T9730 |
T9731 |
nummod |
6,wk |
| R1648 |
T9731 |
T9729 |
pobj |
wk,at |
| R1649 |
T9732 |
T9710 |
punct |
.,observed |
| R1650 |
T9832 |
T9830 |
pobj |
glomeruli,of |
| R1651 |
T9734 |
T9735 |
advmod |
Even,at |
| R1652 |
T9833 |
T9832 |
cc |
and,glomeruli |
| R1653 |
T9735 |
T9736 |
prep |
at,had |
| R1654 |
T9737 |
T9738 |
nummod |
4,wk |
| R1655 |
T9834 |
T9835 |
amod |
proximal,distal |
| R1656 |
T9738 |
T9735 |
pobj |
wk,at |
| R1657 |
T9739 |
T9736 |
punct |
", ",had |
| R1658 |
T9835 |
T9837 |
amod |
distal,tubules |
| R1659 |
T9740 |
T9741 |
compound |
Aqp2F204V,F204V |
| R1660 |
T9741 |
T9743 |
compound |
F204V,mice |
| R1661 |
T9742 |
T9741 |
punct |
/,F204V |
| R1662 |
T9743 |
T9736 |
nsubj |
mice,had |
| R1663 |
T9836 |
T9835 |
punct |
/,distal |
| R1664 |
T9744 |
T9736 |
dobj |
hydronephrosis,had |
| R1665 |
T9745 |
T9736 |
punct |
.,had |
| R1666 |
T9837 |
T9832 |
conj |
tubules,glomeruli |
| R1667 |
T9747 |
T9748 |
amod |
Histologic,sections |
| R1668 |
T9748 |
T9749 |
nsubj |
sections,demonstrated |
| R1669 |
T9838 |
T9825 |
acomp |
unremarkable,were |
| R1670 |
T9750 |
T9748 |
prep |
from,sections |
| R1671 |
T9751 |
T9752 |
compound |
Aqp2F204V,F204V |
| R1672 |
T9839 |
T9840 |
punct |
(,Figure |
| R1673 |
T9752 |
T9754 |
compound |
F204V,mice |
| R1674 |
T9753 |
T9752 |
punct |
/,F204V |
| R1675 |
T9754 |
T9750 |
pobj |
mice,from |
| R1676 |
T9840 |
T9825 |
parataxis |
Figure,were |
| R1677 |
T9755 |
T9756 |
amod |
marked,dilatation |
| R1678 |
T9756 |
T9749 |
dobj |
dilatation,demonstrated |
| R1679 |
T9841 |
T9840 |
nummod |
2D,Figure |
| R1680 |
T9757 |
T9756 |
prep |
of,dilatation |
| R1681 |
T9758 |
T9759 |
det |
the,pelvis |
| R1682 |
T9842 |
T9840 |
punct |
),Figure |
| R1683 |
T9759 |
T9757 |
pobj |
pelvis,of |
| R1684 |
T9760 |
T9759 |
amod |
renal,pelvis |
| R1685 |
T9761 |
T9756 |
cc |
yet,dilatation |
| R1686 |
T9762 |
T9763 |
amod |
normal,morphology |
| R1687 |
T9843 |
T9825 |
punct |
.,were |
| R1688 |
T9763 |
T9756 |
conj |
morphology,dilatation |
| R1689 |
T9764 |
T9763 |
prep |
of,morphology |
| R1690 |
T9765 |
T9766 |
det |
the,ureter |
| R1691 |
T9845 |
T9846 |
mark |
As,shown |
| R1692 |
T9766 |
T9764 |
pobj |
ureter,of |
| R1693 |
T9767 |
T9768 |
punct |
(,2C |
| R1694 |
T9768 |
T9749 |
parataxis |
2C,demonstrated |
| R1695 |
T9846 |
T9847 |
advcl |
shown,revealed |
| R1696 |
T9769 |
T9768 |
nmod |
Figure,2C |
| R1697 |
T9770 |
T9768 |
cc |
and,2C |
| R1698 |
T9771 |
T9768 |
conj |
2D,2C |
| R1699 |
T9848 |
T9846 |
advmod |
previously,shown |
| R1700 |
T9772 |
T9768 |
punct |
),2C |
| R1701 |
T9773 |
T9749 |
punct |
.,demonstrated |
| R1702 |
T9849 |
T9850 |
punct |
[,22 |
| R1703 |
T9775 |
T9776 |
prep |
In,was |
| R1704 |
T9777 |
T9775 |
amod |
particular,In |
| R1705 |
T9778 |
T9776 |
punct |
", ",was |
| R1706 |
T9779 |
T9780 |
det |
the,propria |
| R1707 |
T9850 |
T9846 |
parataxis |
22,shown |
| R1708 |
T9780 |
T9776 |
nsubj |
propria,was |
| R1709 |
T9781 |
T9780 |
compound |
muscularis,propria |
| R1710 |
T9782 |
T9783 |
preconj |
neither,hypertrophied |
| R1711 |
T9851 |
T9850 |
nummod |
18,22 |
| R1712 |
T9852 |
T9850 |
punct |
",",22 |
| R1713 |
T9783 |
T9776 |
acomp |
hypertrophied,was |
| R1714 |
T9853 |
T9850 |
punct |
],22 |
| R1715 |
T9784 |
T9783 |
cc |
nor,hypertrophied |
| R1716 |
T9785 |
T9783 |
conj |
thinned,hypertrophied |
| R1717 |
T9854 |
T9847 |
punct |
", ",revealed |
| R1718 |
T9786 |
T9776 |
punct |
.,was |
| R1719 |
T9855 |
T9847 |
nsubj |
immunoblotting,revealed |
| R1720 |
T9788 |
T9789 |
expl |
There,was |
| R1721 |
T9790 |
T9791 |
det |
the,appearance |
| R1722 |
T9856 |
T9857 |
nummod |
three,forms |
| R1723 |
T9791 |
T9789 |
attr |
appearance,was |
| R1724 |
T9792 |
T9791 |
amod |
normal,appearance |
| R1725 |
T9857 |
T9847 |
dobj |
forms,revealed |
| R1726 |
T9793 |
T9791 |
amod |
festooned,appearance |
| R1727 |
T9794 |
T9791 |
prep |
of,appearance |
| R1728 |
T9795 |
T9796 |
det |
the,urothelium |
| R1729 |
T9796 |
T9794 |
pobj |
urothelium,of |
| R1730 |
T9858 |
T9857 |
amod |
different,forms |
| R1731 |
T9797 |
T9789 |
punct |
", ",was |
| R1732 |
T9798 |
T9789 |
cc |
and,was |
| R1733 |
T9859 |
T9857 |
prep |
of,forms |
| R1734 |
T9799 |
T9800 |
det |
this,epithelium |
| R1735 |
T9800 |
T9802 |
nsubj |
epithelium,was |
| R1736 |
T9801 |
T9800 |
amod |
transitional,epithelium |
| R1737 |
T9802 |
T9789 |
conj |
was,was |
| R1738 |
T9803 |
T9802 |
prep |
of,was |
| R1739 |
T9804 |
T9805 |
amod |
normal,thickness |
| R1740 |
T9860 |
T9859 |
pobj |
AQP2,of |
| R1741 |
T9805 |
T9803 |
pobj |
thickness,of |
| R1742 |
T9806 |
T9802 |
punct |
.,was |
| R1743 |
T9861 |
T9847 |
punct |
", ",revealed |
| R1744 |
T9808 |
T9809 |
expl |
There,was |
| R1745 |
T9810 |
T9809 |
attr |
thinning,was |
| R1746 |
T9862 |
T9847 |
prep |
due,revealed |
| R1747 |
T9811 |
T9810 |
prep |
of,thinning |
| R1748 |
T9812 |
T9813 |
det |
the,kidney |
| R1749 |
T9813 |
T9811 |
pobj |
kidney,of |
| R1750 |
T9863 |
T9862 |
pcomp |
to,due |
| R1751 |
T9814 |
T9815 |
mark |
as,measured |
| R1752 |
T9815 |
T9809 |
advcl |
measured,was |
| R1753 |
T9816 |
T9815 |
prep |
from,measured |
| R1754 |
T9864 |
T9865 |
amod |
different,degrees |
| R1755 |
T9817 |
T9818 |
amod |
renal,capsule |
| R1756 |
T9818 |
T9816 |
pobj |
capsule,from |
| R1757 |
T9819 |
T9816 |
prep |
to,from |
| R1758 |
T9865 |
T9862 |
pobj |
degrees,due |
| R1759 |
T9820 |
T9821 |
amod |
renal,pelvis |
| R1760 |
T9821 |
T9819 |
pobj |
pelvis,to |
| R1761 |
T9866 |
T9865 |
cc |
and,degrees |
| R1762 |
T9822 |
T9809 |
punct |
.,was |
| R1763 |
T9824 |
T9825 |
advmod |
However,were |
| R1764 |
T9867 |
T9865 |
conj |
forms,degrees |
| R1765 |
T9826 |
T9825 |
punct |
", ",were |
| R1766 |
T9868 |
T9865 |
prep |
of,degrees |
| R1767 |
T9869 |
T9868 |
pobj |
glycosylation,of |
| R1768 |
T9870 |
T9871 |
punct |
(,Figure |
| R1769 |
T9871 |
T9847 |
parataxis |
Figure,revealed |
| R1770 |
T9872 |
T9871 |
nummod |
3A,Figure |
| R1771 |
T9933 |
T9932 |
punct |
],22 |
| R1772 |
T9873 |
T9871 |
punct |
),Figure |
| R1773 |
T9934 |
T9911 |
punct |
.,is |
| R1774 |
T9874 |
T9847 |
punct |
.,revealed |
| R1775 |
T9936 |
T9937 |
prep |
Compared,reduced |
| R1776 |
T9876 |
T9877 |
amod |
Previous,reports |
| R1777 |
T9938 |
T9936 |
prep |
to,Compared |
| R1778 |
T9939 |
T9938 |
pobj |
that,to |
| R1779 |
T9940 |
T9939 |
prep |
from,that |
| R1780 |
T9941 |
T9942 |
det |
the,kidneys |
| R1781 |
T9877 |
T9878 |
nsubj |
reports,demonstrated |
| R1782 |
T9942 |
T9940 |
pobj |
kidneys,from |
| R1783 |
T9943 |
T9942 |
prep |
of,kidneys |
| R1784 |
T9879 |
T9878 |
aux |
have,demonstrated |
| R1785 |
T9944 |
T9945 |
amod |
wild,type |
| R1786 |
T9945 |
T9947 |
compound |
type,animals |
| R1787 |
T9946 |
T9945 |
punct |
-,type |
| R1788 |
T9947 |
T9943 |
pobj |
animals,of |
| R1789 |
T9948 |
T9937 |
punct |
", ",reduced |
| R1790 |
T9949 |
T9937 |
nsubjpass |
AQP2,reduced |
| R1791 |
T9880 |
T9881 |
mark |
that,appears |
| R1792 |
T9950 |
T9949 |
prep |
from,AQP2 |
| R1793 |
T9951 |
T9952 |
compound |
mutant,animals |
| R1794 |
T9952 |
T9950 |
pobj |
animals,from |
| R1795 |
T9881 |
T9878 |
ccomp |
appears,demonstrated |
| R1796 |
T9953 |
T9937 |
auxpass |
was,reduced |
| R1797 |
T9954 |
T9937 |
prep |
in,reduced |
| R1798 |
T9955 |
T9956 |
preconj |
both,form |
| R1799 |
T9882 |
T9883 |
amod |
nonglycosylated,protein |
| R1800 |
T9956 |
T9954 |
pobj |
form,in |
| R1801 |
T9957 |
T9956 |
det |
the,form |
| R1802 |
T9883 |
T9881 |
nsubj |
protein,appears |
| R1803 |
T9958 |
T9959 |
amod |
high,weight |
| R1804 |
T9959 |
T9956 |
nmod |
weight,form |
| R1805 |
T9960 |
T9959 |
amod |
molecular,weight |
| R1806 |
T9884 |
T9881 |
prep |
as,appears |
| R1807 |
T9961 |
T9956 |
punct |
", ",form |
| R1808 |
T9962 |
T9956 |
amod |
diffuse,form |
| R1809 |
T9963 |
T9956 |
cc |
and,form |
| R1810 |
T9885 |
T9886 |
det |
a,band |
| R1811 |
T9964 |
T9965 |
det |
the,form |
| R1812 |
T9965 |
T9956 |
conj |
form,form |
| R1813 |
T9966 |
T9967 |
amod |
lowest,weight |
| R1814 |
T9886 |
T9884 |
pobj |
band,as |
| R1815 |
T9967 |
T9965 |
compound |
weight,form |
| R1816 |
T9968 |
T9967 |
amod |
molecular,weight |
| R1817 |
T9969 |
T9937 |
punct |
", ",reduced |
| R1818 |
T9887 |
T9888 |
nummod |
29,kDa |
| R1819 |
T9970 |
T9937 |
cc |
but,reduced |
| R1820 |
T9971 |
T9937 |
conj |
enriched,reduced |
| R1821 |
T9888 |
T9886 |
compound |
kDa,band |
| R1822 |
T9972 |
T9971 |
prep |
in,enriched |
| R1823 |
T9973 |
T9974 |
det |
the,form |
| R1824 |
T9974 |
T9972 |
pobj |
form,in |
| R1825 |
T9889 |
T9881 |
punct |
", ",appears |
| R1826 |
T9975 |
T9976 |
amod |
intermediate,weight |
| R1827 |
T9976 |
T9974 |
compound |
weight,form |
| R1828 |
T9977 |
T9976 |
amod |
molecular,weight |
| R1829 |
T9890 |
T9891 |
mark |
while,runs |
| R1830 |
T9978 |
T9979 |
punct |
(,Figure |
| R1831 |
T9979 |
T9971 |
parataxis |
Figure,enriched |
| R1832 |
T9980 |
T9979 |
nummod |
3A,Figure |
| R1833 |
T9891 |
T9881 |
advcl |
runs,appears |
| R1834 |
T9981 |
T9979 |
punct |
),Figure |
| R1835 |
T9982 |
T9937 |
punct |
.,reduced |
| R1836 |
T9892 |
T9893 |
amod |
complex,protein |
| R1837 |
T9984 |
T9985 |
amod |
Heterozygous,animals |
| R1838 |
T9985 |
T9986 |
nsubj |
animals,showed |
| R1839 |
T9987 |
T9988 |
amod |
intermediate,amounts |
| R1840 |
T9988 |
T9986 |
dobj |
amounts,showed |
| R1841 |
T9893 |
T9891 |
nsubj |
protein,runs |
| R1842 |
T9989 |
T9988 |
prep |
of,amounts |
| R1843 |
T9990 |
T9991 |
det |
all,forms |
| R1844 |
T9991 |
T9989 |
pobj |
forms,of |
| R1845 |
T9894 |
T9893 |
amod |
glycosylated,protein |
| R1846 |
T9992 |
T9991 |
nummod |
three,forms |
| R1847 |
T9993 |
T9986 |
punct |
.,showed |
| R1848 |
T9895 |
T9891 |
prep |
as,runs |
| R1849 |
T9995 |
T9996 |
det |
The,nature |
| R1850 |
T9996 |
T9997 |
nsubjpass |
nature,revealed |
| R1851 |
T9896 |
T9897 |
det |
a,smear |
| R1852 |
T9998 |
T9996 |
prep |
of,nature |
| R1853 |
T9999 |
T10000 |
det |
these,forms |
| R1854 |
T9897 |
T9895 |
pobj |
smear,as |
| R1855 |
T10000 |
T9998 |
pobj |
forms,of |
| R1856 |
T10001 |
T10000 |
amod |
glycosylated,forms |
| R1857 |
T9898 |
T9897 |
prep |
between,smear |
| R1858 |
T10002 |
T9997 |
auxpass |
was,revealed |
| R1859 |
T10003 |
T9997 |
prep |
by,revealed |
| R1860 |
T10004 |
T10003 |
pobj |
digestion,by |
| R1861 |
T9899 |
T9900 |
nummod |
35,kDa |
| R1862 |
T10005 |
T10004 |
prep |
with,digestion |
| R1863 |
T10006 |
T10007 |
compound |
endoglycosidase,H |
| R1864 |
T10007 |
T10005 |
pobj |
H,with |
| R1865 |
T9900 |
T9898 |
pobj |
kDa,between |
| R1866 |
T10008 |
T10007 |
punct |
", ",H |
| R1867 |
T10009 |
T10010 |
dep |
which,cleaves |
| R1868 |
T9901 |
T9899 |
cc |
and,35 |
| R1869 |
T10010 |
T10007 |
relcl |
cleaves,H |
| R1870 |
T10011 |
T10010 |
advmod |
specifically,cleaves |
| R1871 |
T10012 |
T10013 |
npadvmod |
mannose,rich |
| R1872 |
T9902 |
T9899 |
conj |
45,35 |
| R1873 |
T10013 |
T10015 |
amod |
rich,carbohydrate |
| R1874 |
T10014 |
T10013 |
punct |
-,rich |
| R1875 |
T10015 |
T10010 |
dobj |
carbohydrate,cleaves |
| R1876 |
T9903 |
T9878 |
punct |
.,demonstrated |
| R1877 |
T10016 |
T10010 |
prep |
from,cleaves |
| R1878 |
T10017 |
T10018 |
det |
the,backbone |
| R1879 |
T10018 |
T10016 |
pobj |
backbone,from |
| R1880 |
T9905 |
T9906 |
det |
A,form |
| R1881 |
T10019 |
T10018 |
compound |
protein,backbone |
| R1882 |
T9906 |
T9911 |
nsubj |
form,is |
| R1883 |
T10020 |
T9997 |
punct |
.,revealed |
| R1884 |
T9907 |
T9908 |
amod |
short,lived |
| R1885 |
T10022 |
T10023 |
nsubj |
Treatment,affected |
| R1886 |
T9908 |
T9906 |
amod |
lived,form |
| R1887 |
T10024 |
T10022 |
prep |
of,Treatment |
| R1888 |
T10025 |
T10026 |
amod |
endogenous,AQP2 |
| R1889 |
T10026 |
T10024 |
pobj |
AQP2,of |
| R1890 |
T9909 |
T9908 |
punct |
-,lived |
| R1891 |
T10027 |
T10026 |
prep |
from,AQP2 |
| R1892 |
T10028 |
T10027 |
pobj |
kidneys,from |
| R1893 |
T10029 |
T10028 |
prep |
of,kidneys |
| R1894 |
T10030 |
T10031 |
amod |
wild,type |
| R1895 |
T10031 |
T10033 |
nmod |
type,animals |
| R1896 |
T9910 |
T9906 |
amod |
intermediate,form |
| R1897 |
T10032 |
T10031 |
punct |
-,type |
| R1898 |
T10033 |
T10029 |
pobj |
animals,of |
| R1899 |
T9912 |
T9906 |
prep |
of,form |
| R1900 |
T10034 |
T10031 |
punct |
", ",type |
| R1901 |
T10035 |
T10031 |
conj |
heterozygous,type |
| R1902 |
T10036 |
T10035 |
punct |
", ",heterozygous |
| R1903 |
T10037 |
T10035 |
cc |
and,heterozygous |
| R1904 |
T9913 |
T9914 |
nummod |
31,kDa |
| R1905 |
T10038 |
T10035 |
conj |
mutant,heterozygous |
| R1906 |
T9914 |
T9912 |
pobj |
kDa,of |
| R1907 |
T9915 |
T9906 |
acl |
representing,form |
| R1908 |
T9916 |
T9917 |
nmod |
core,glycosylation |
| R1909 |
T10039 |
T10023 |
advmod |
specifically,affected |
| R1910 |
T10040 |
T10041 |
det |
the,form |
| R1911 |
T9917 |
T9915 |
dobj |
glycosylation,representing |
| R1912 |
T10041 |
T10023 |
dobj |
form,affected |
| R1913 |
T10042 |
T10043 |
amod |
intermediate,weight |
| R1914 |
T10043 |
T10041 |
compound |
weight,form |
| R1915 |
T9918 |
T9917 |
punct |
", ",glycosylation |
| R1916 |
T10044 |
T10043 |
amod |
molecular,weight |
| R1917 |
T10045 |
T10046 |
punct |
(,Figure |
| R1918 |
T9919 |
T9920 |
amod |
high,mannose |
| R1919 |
T10046 |
T10023 |
parataxis |
Figure,affected |
| R1920 |
T10047 |
T10046 |
nummod |
3B,Figure |
| R1921 |
T10048 |
T10046 |
punct |
),Figure |
| R1922 |
T9920 |
T9917 |
compound |
mannose,glycosylation |
| R1923 |
T10049 |
T10023 |
punct |
.,affected |
| R1924 |
T10051 |
T10052 |
det |
The,presence |
| R1925 |
T9921 |
T9920 |
punct |
-,mannose |
| R1926 |
T10052 |
T10053 |
nsubj |
presence,permits |
| R1927 |
T9922 |
T9917 |
prep |
of,glycosylation |
| R1928 |
T10054 |
T10052 |
prep |
of,presence |
| R1929 |
T10055 |
T10056 |
det |
some,proteins |
| R1930 |
T10056 |
T10054 |
pobj |
proteins,of |
| R1931 |
T9923 |
T9922 |
pobj |
AQP2,of |
| R1932 |
T10057 |
T10056 |
amod |
mature,proteins |
| R1933 |
T10058 |
T10056 |
amod |
glycosylated,proteins |
| R1934 |
T10059 |
T10060 |
punct |
(,kDa |
| R1935 |
T9924 |
T9911 |
acomp |
apparent,is |
| R1936 |
T10060 |
T10056 |
parataxis |
kDa,proteins |
| R1937 |
T10061 |
T10062 |
quantmod |
35,45 |
| R1938 |
T10062 |
T10060 |
nummod |
45,kDa |
| R1939 |
T9925 |
T9924 |
prep |
from,apparent |
| R1940 |
T10063 |
T10062 |
punct |
–,45 |
| R1941 |
T10064 |
T10060 |
punct |
),kDa |
| R1942 |
T9926 |
T9927 |
compound |
pulse,chase |
| R1943 |
T10065 |
T10056 |
prep |
in,proteins |
| R1944 |
T10066 |
T10067 |
compound |
Aqp2F204V,F204V |
| R1945 |
T10067 |
T10069 |
compound |
F204V,mice |
| R1946 |
T10068 |
T10067 |
punct |
/,F204V |
| R1947 |
T10069 |
T10065 |
pobj |
mice,in |
| R1948 |
T10070 |
T10053 |
advmod |
presumably,permits |
| R1949 |
T9927 |
T9929 |
compound |
chase,labeling |
| R1950 |
T10071 |
T10072 |
poss |
their,survival |
| R1951 |
T10072 |
T10053 |
dobj |
survival,permits |
| R1952 |
T10073 |
T10072 |
prep |
compared,survival |
| R1953 |
T9928 |
T9927 |
punct |
-,chase |
| R1954 |
T10074 |
T10073 |
prep |
to,compared |
| R1955 |
T10075 |
T10076 |
compound |
Aqp2T126M,T126M |
| R1956 |
T10076 |
T10078 |
compound |
T126M,mice |
| R1957 |
T9929 |
T9930 |
compound |
labeling,experiments |
| R1958 |
T10077 |
T10076 |
punct |
/,T126M |
| R1959 |
T10078 |
T10074 |
pobj |
mice,to |
| R1960 |
T10079 |
T10053 |
punct |
", ",permits |
| R1961 |
T9930 |
T9925 |
pobj |
experiments,from |
| R1962 |
T10080 |
T10053 |
cc |
and,permits |
| R1963 |
T10081 |
T10053 |
conj |
is,permits |
| R1964 |
T10082 |
T10081 |
acomp |
consistent,is |
| R1965 |
T9931 |
T9932 |
punct |
[,22 |
| R1966 |
T10083 |
T10082 |
prep |
with,consistent |
| R1967 |
T10084 |
T10085 |
det |
a,response |
| R1968 |
T9932 |
T9911 |
parataxis |
22,is |
| R1969 |
T10085 |
T10083 |
pobj |
response,with |
| R1970 |
T10086 |
T10085 |
amod |
diminished,response |
| R1971 |
T10087 |
T10085 |
prep |
to,response |
| R1972 |
T10088 |
T10087 |
pobj |
dDAVP,to |
| R1973 |
T10146 |
T10147 |
prep |
In,fail |
| R1974 |
T10089 |
T10053 |
punct |
.,permits |
| R1975 |
T10091 |
T10092 |
prep |
In,postulated |
| R1976 |
T10093 |
T10091 |
pobj |
humans,In |
| R1977 |
T10148 |
T10146 |
amod |
general,In |
| R1978 |
T10094 |
T10092 |
punct |
", ",postulated |
| R1979 |
T10095 |
T10096 |
amod |
recessive,alleles |
| R1980 |
T10096 |
T10092 |
nsubjpass |
alleles,postulated |
| R1981 |
T10097 |
T10096 |
prep |
of,alleles |
| R1982 |
T10149 |
T10147 |
punct |
", ",fail |
| R1983 |
T10098 |
T10097 |
pobj |
Aqp2,of |
| R1984 |
T10099 |
T10092 |
auxpass |
are,postulated |
| R1985 |
T10100 |
T10101 |
aux |
to,cause |
| R1986 |
T10150 |
T10151 |
amod |
such,alleles |
| R1987 |
T10101 |
T10092 |
xcomp |
cause,postulated |
| R1988 |
T10102 |
T10101 |
dobj |
NDI,cause |
| R1989 |
T10103 |
T10104 |
mark |
because,translocate |
| R1990 |
T10151 |
T10147 |
nsubj |
alleles,fail |
| R1991 |
T10152 |
T10151 |
amod |
recessive,alleles |
| R1992 |
T10104 |
T10101 |
advcl |
translocate,cause |
| R1993 |
T10105 |
T10104 |
nsubj |
they,translocate |
| R1994 |
T10153 |
T10147 |
punct |
", ",fail |
| R1995 |
T10106 |
T10104 |
aux |
do,translocate |
| R1996 |
T10107 |
T10104 |
neg |
not,translocate |
| R1997 |
T10108 |
T10104 |
advmod |
properly,translocate |
| R1998 |
T10109 |
T10104 |
prep |
to,translocate |
| R1999 |
T10110 |
T10111 |
det |
the,surface |
| R2000 |
T10154 |
T10155 |
advmod |
when,visualized |
| R2001 |
T10111 |
T10109 |
pobj |
surface,to |
| R2002 |
T10112 |
T10111 |
amod |
apical,surface |
| R2003 |
T10113 |
T10111 |
compound |
cell,surface |
| R2004 |
T10114 |
T10104 |
prep |
in,translocate |
| R2005 |
T10155 |
T10147 |
advcl |
visualized,fail |
| R2006 |
T10115 |
T10114 |
pobj |
response,in |
| R2007 |
T10116 |
T10115 |
prep |
to,response |
| R2008 |
T10156 |
T10155 |
advmod |
immunocytochemically,visualized |
| R2009 |
T10117 |
T10116 |
pobj |
AVP,to |
| R2010 |
T10118 |
T10092 |
punct |
.,postulated |
| R2011 |
T10157 |
T10147 |
punct |
", ",fail |
| R2012 |
T10120 |
T10121 |
det |
This,postulate |
| R2013 |
T10121 |
T10122 |
nsubj |
postulate,comes |
| R2014 |
T10158 |
T10159 |
aux |
to,localize |
| R2015 |
T10123 |
T10124 |
advmod |
solely,from |
| R2016 |
T10124 |
T10122 |
prep |
from,comes |
| R2017 |
T10159 |
T10147 |
xcomp |
localize,fail |
| R2018 |
T10125 |
T10126 |
advmod |
in,vitro |
| R2019 |
T10126 |
T10127 |
amod |
vitro,studies |
| R2020 |
T10127 |
T10124 |
pobj |
studies,from |
| R2021 |
T10160 |
T10159 |
prep |
to,localize |
| R2022 |
T10128 |
T10129 |
prep |
in,transfected |
| R2023 |
T10129 |
T10127 |
relcl |
transfected,studies |
| R2024 |
T10130 |
T10128 |
pobj |
which,in |
| R2025 |
T10161 |
T10162 |
npadvmod |
AVP,responsive |
| R2026 |
T10131 |
T10132 |
compound |
mutant,cDNAs |
| R2027 |
T10132 |
T10129 |
nsubjpass |
cDNAs,transfected |
| R2028 |
T10133 |
T10132 |
compound |
Aqp2,cDNAs |
| R2029 |
T10162 |
T10164 |
amod |
responsive,vesicles |
| R2030 |
T10134 |
T10132 |
acl |
corresponding,cDNAs |
| R2031 |
T10135 |
T10134 |
prep |
to,corresponding |
| R2032 |
T10136 |
T10137 |
amod |
human,mutations |
| R2033 |
T10163 |
T10162 |
punct |
-,responsive |
| R2034 |
T10137 |
T10135 |
pobj |
mutations,to |
| R2035 |
T10138 |
T10137 |
compound |
disease,mutations |
| R2036 |
T10164 |
T10160 |
pobj |
vesicles,to |
| R2037 |
T10139 |
T10129 |
auxpass |
are,transfected |
| R2038 |
T10140 |
T10129 |
prep |
into,transfected |
| R2039 |
T10141 |
T10142 |
compound |
kidney,lines |
| R2040 |
T10165 |
T10147 |
punct |
.,fail |
| R2041 |
T10142 |
T10140 |
pobj |
lines,into |
| R2042 |
T10143 |
T10142 |
compound |
cell,lines |
| R2043 |
T10144 |
T10122 |
punct |
.,comes |
| R2044 |
T10167 |
T10168 |
advmod |
Rather,trapped |
| R2045 |
T10169 |
T10168 |
punct |
", ",trapped |
| R2046 |
T10170 |
T10168 |
nsubjpass |
they,trapped |
| R2047 |
T10171 |
T10168 |
auxpass |
get,trapped |
| R2048 |
T10172 |
T10168 |
prep |
in,trapped |
| R2049 |
T10250 |
T10237 |
parataxis |
row,translocated |
| R2050 |
T10251 |
T10250 |
dep |
Figure,row |
| R2051 |
T10173 |
T10174 |
det |
the,reticulum |
| R2052 |
T10252 |
T10251 |
nummod |
4A,Figure |
| R2053 |
T10253 |
T10250 |
punct |
", ",row |
| R2054 |
T10174 |
T10172 |
pobj |
reticulum,in |
| R2055 |
T10254 |
T10250 |
amod |
second,row |
| R2056 |
T10255 |
T10250 |
punct |
),row |
| R2057 |
T10256 |
T10237 |
punct |
.,translocated |
| R2058 |
T10175 |
T10174 |
amod |
endoplasmic,reticulum |
| R2059 |
T10258 |
T10259 |
prep |
In,distributed |
| R2060 |
T10176 |
T10174 |
punct |
(,reticulum |
| R2061 |
T10260 |
T10258 |
pobj |
kidneys,In |
| R2062 |
T10261 |
T10260 |
acl |
taken,kidneys |
| R2063 |
T10177 |
T10174 |
appos |
ER,reticulum |
| R2064 |
T10262 |
T10261 |
prep |
from,taken |
| R2065 |
T10263 |
T10264 |
compound |
mutant,animals |
| R2066 |
T10264 |
T10262 |
pobj |
animals,from |
| R2067 |
T10178 |
T10168 |
punct |
),trapped |
| R2068 |
T10265 |
T10259 |
punct |
", ",distributed |
| R2069 |
T10266 |
T10259 |
advmod |
however,distributed |
| R2070 |
T10267 |
T10259 |
punct |
", ",distributed |
| R2071 |
T10179 |
T10168 |
punct |
.,trapped |
| R2072 |
T10268 |
T10259 |
nsubjpass |
AQP2,distributed |
| R2073 |
T10269 |
T10259 |
auxpass |
was,distributed |
| R2074 |
T10270 |
T10259 |
advmod |
randomly,distributed |
| R2075 |
T10271 |
T10259 |
prep |
throughout,distributed |
| R2076 |
T10181 |
T10182 |
poss |
Our,model |
| R2077 |
T10272 |
T10273 |
det |
the,cell |
| R2078 |
T10273 |
T10271 |
pobj |
cell,throughout |
| R2079 |
T10274 |
T10259 |
prep |
in,distributed |
| R2080 |
T10275 |
T10276 |
det |
the,state |
| R2081 |
T10182 |
T10184 |
nsubj |
model,affords |
| R2082 |
T10276 |
T10274 |
pobj |
state,in |
| R2083 |
T10277 |
T10276 |
amod |
basal,state |
| R2084 |
T10278 |
T10279 |
punct |
(,row |
| R2085 |
T10183 |
T10182 |
compound |
mouse,model |
| R2086 |
T10185 |
T10182 |
prep |
of,model |
| R2087 |
T10279 |
T10259 |
parataxis |
row,distributed |
| R2088 |
T10186 |
T10185 |
pobj |
NDI,of |
| R2089 |
T10280 |
T10279 |
dep |
Figure,row |
| R2090 |
T10281 |
T10280 |
nummod |
4A,Figure |
| R2091 |
T10187 |
T10188 |
det |
the,opportunity |
| R2092 |
T10282 |
T10279 |
punct |
", ",row |
| R2093 |
T10283 |
T10279 |
amod |
third,row |
| R2094 |
T10284 |
T10279 |
punct |
),row |
| R2095 |
T10188 |
T10184 |
dobj |
opportunity,affords |
| R2096 |
T10285 |
T10259 |
punct |
", ",distributed |
| R2097 |
T10286 |
T10287 |
mark |
while,localized |
| R2098 |
T10189 |
T10188 |
amod |
first,opportunity |
| R2099 |
T10287 |
T10259 |
advcl |
localized,distributed |
| R2100 |
T10288 |
T10287 |
nsubj |
AQP3,localized |
| R2101 |
T10289 |
T10290 |
punct |
(,green |
| R2102 |
T10290 |
T10288 |
parataxis |
green,AQP3 |
| R2103 |
T10291 |
T10290 |
punct |
),green |
| R2104 |
T10190 |
T10191 |
aux |
to,test |
| R2105 |
T10292 |
T10287 |
advmod |
appropriately,localized |
| R2106 |
T10293 |
T10287 |
prep |
to,localized |
| R2107 |
T10294 |
T10295 |
det |
the,surface |
| R2108 |
T10191 |
T10188 |
acl |
test,opportunity |
| R2109 |
T10295 |
T10293 |
pobj |
surface,to |
| R2110 |
T10296 |
T10295 |
amod |
basolateral,surface |
| R2111 |
T10297 |
T10298 |
punct |
[,23 |
| R2112 |
T10192 |
T10193 |
det |
this,hypothesis |
| R2113 |
T10298 |
T10287 |
parataxis |
23,localized |
| R2114 |
T10299 |
T10298 |
punct |
],23 |
| R2115 |
T10300 |
T10259 |
punct |
.,distributed |
| R2116 |
T10193 |
T10191 |
dobj |
hypothesis,test |
| R2117 |
T10302 |
T10303 |
advmod |
Furthermore,failed |
| R2118 |
T10194 |
T10191 |
prep |
in,test |
| R2119 |
T10304 |
T10303 |
punct |
", ",failed |
| R2120 |
T10305 |
T10303 |
prep |
upon,failed |
| R2121 |
T10306 |
T10307 |
compound |
dDAVP,stimulation |
| R2122 |
T10195 |
T10196 |
det |
a,animal |
| R2123 |
T10307 |
T10305 |
pobj |
stimulation,upon |
| R2124 |
T10308 |
T10303 |
punct |
", ",failed |
| R2125 |
T10309 |
T10310 |
compound |
AQP2,F204V |
| R2126 |
T10196 |
T10194 |
pobj |
animal,in |
| R2127 |
T10310 |
T10303 |
nsubj |
F204V,failed |
| R2128 |
T10311 |
T10310 |
punct |
-,F204V |
| R2129 |
T10312 |
T10313 |
aux |
to,translocate |
| R2130 |
T10197 |
T10196 |
amod |
mature,animal |
| R2131 |
T10313 |
T10303 |
xcomp |
translocate,failed |
| R2132 |
T10314 |
T10313 |
prep |
to,translocate |
| R2133 |
T10198 |
T10184 |
punct |
.,affords |
| R2134 |
T10315 |
T10316 |
det |
the,surface |
| R2135 |
T10316 |
T10314 |
pobj |
surface,to |
| R2136 |
T10317 |
T10316 |
compound |
cell,surface |
| R2137 |
T10200 |
T10201 |
mark |
As,shown |
| R2138 |
T10318 |
T10319 |
punct |
(,row |
| R2139 |
T10319 |
T10303 |
parataxis |
row,failed |
| R2140 |
T10320 |
T10319 |
dep |
Figure,row |
| R2141 |
T10321 |
T10320 |
nummod |
4A,Figure |
| R2142 |
T10322 |
T10319 |
punct |
", ",row |
| R2143 |
T10201 |
T10202 |
advcl |
shown,localized |
| R2144 |
T10323 |
T10319 |
amod |
bottom,row |
| R2145 |
T10324 |
T10319 |
punct |
),row |
| R2146 |
T10203 |
T10201 |
prep |
in,shown |
| R2147 |
T10325 |
T10303 |
punct |
.,failed |
| R2148 |
T10327 |
T10328 |
aux |
To,confirm |
| R2149 |
T10328 |
T10329 |
advcl |
confirm,repeated |
| R2150 |
T10204 |
T10203 |
pobj |
Figure,in |
| R2151 |
T10330 |
T10331 |
det |
these,findings |
| R2152 |
T10331 |
T10328 |
dobj |
findings,confirm |
| R2153 |
T10332 |
T10329 |
punct |
", ",repeated |
| R2154 |
T10333 |
T10334 |
det |
the,staining |
| R2155 |
T10334 |
T10329 |
nsubjpass |
staining,repeated |
| R2156 |
T10205 |
T10204 |
nummod |
4A,Figure |
| R2157 |
T10335 |
T10329 |
auxpass |
was,repeated |
| R2158 |
T10336 |
T10329 |
prep |
in,repeated |
| R2159 |
T10337 |
T10336 |
pobj |
kidneys,in |
| R2160 |
T10206 |
T10207 |
punct |
(,row |
| R2161 |
T10338 |
T10337 |
acl |
taken,kidneys |
| R2162 |
T10339 |
T10338 |
prep |
from,taken |
| R2163 |
T10340 |
T10341 |
nummod |
two,mice |
| R2164 |
T10341 |
T10339 |
pobj |
mice,from |
| R2165 |
T10207 |
T10204 |
parataxis |
row,Figure |
| R2166 |
T10342 |
T10341 |
amod |
further,mice |
| R2167 |
T10343 |
T10338 |
prep |
for,taken |
| R2168 |
T10208 |
T10207 |
amod |
top,row |
| R2169 |
T10344 |
T10345 |
det |
each,class |
| R2170 |
T10345 |
T10343 |
pobj |
class,for |
| R2171 |
T10209 |
T10207 |
prep |
of,row |
| R2172 |
T10346 |
T10345 |
punct |
", ",class |
| R2173 |
T10347 |
T10348 |
amod |
wild,type |
| R2174 |
T10348 |
T10345 |
appos |
type,class |
| R2175 |
T10210 |
T10209 |
pobj |
photomicrographs,of |
| R2176 |
T10349 |
T10348 |
punct |
-,type |
| R2177 |
T10350 |
T10348 |
cc |
or,type |
| R2178 |
T10351 |
T10348 |
conj |
mutant,type |
| R2179 |
T10211 |
T10207 |
punct |
),row |
| R2180 |
T10352 |
T10329 |
punct |
", ",repeated |
| R2181 |
T10353 |
T10329 |
prep |
with,repeated |
| R2182 |
T10354 |
T10353 |
cc |
or,with |
| R2183 |
T10212 |
T10202 |
punct |
", ",localized |
| R2184 |
T10355 |
T10353 |
conj |
without,with |
| R2185 |
T10213 |
T10202 |
nsubj |
AQP2,localized |
| R2186 |
T10214 |
T10213 |
punct |
(,AQP2 |
| R2187 |
T10215 |
T10213 |
acl |
stained,AQP2 |
| R2188 |
T10356 |
T10357 |
compound |
dDAVP,treatment |
| R2189 |
T10357 |
T10355 |
pobj |
treatment,without |
| R2190 |
T10216 |
T10215 |
oprd |
red,stained |
| R2191 |
T10358 |
T10329 |
punct |
", ",repeated |
| R2192 |
T10359 |
T10329 |
prep |
with,repeated |
| R2193 |
T10217 |
T10213 |
punct |
),AQP2 |
| R2194 |
T10360 |
T10361 |
amod |
identical,results |
| R2195 |
T10361 |
T10359 |
pobj |
results,with |
| R2196 |
T10362 |
T10329 |
punct |
.,repeated |
| R2197 |
T10218 |
T10202 |
advmod |
normally,localized |
| R2198 |
T10364 |
T10365 |
aux |
To,investigate |
| R2199 |
T10365 |
T10366 |
advcl |
investigate,turned |
| R2200 |
T10219 |
T10202 |
prep |
to,localized |
| R2201 |
T10367 |
T10368 |
det |
the,mechanism |
| R2202 |
T10220 |
T10221 |
det |
the,region |
| R2203 |
T10368 |
T10365 |
dobj |
mechanism,investigate |
| R2204 |
T10221 |
T10219 |
pobj |
region,to |
| R2205 |
T10369 |
T10368 |
prep |
of,mechanism |
| R2206 |
T10370 |
T10371 |
amod |
defective,translocation |
| R2207 |
T10222 |
T10221 |
amod |
subapical,region |
| R2208 |
T10371 |
T10369 |
pobj |
translocation,of |
| R2209 |
T10372 |
T10371 |
prep |
of,translocation |
| R2210 |
T10223 |
T10221 |
prep |
of,region |
| R2211 |
T10373 |
T10374 |
compound |
AQP2,F204V |
| R2212 |
T10374 |
T10372 |
pobj |
F204V,of |
| R2213 |
T10375 |
T10374 |
punct |
-,F204V |
| R2214 |
T10224 |
T10225 |
amod |
collecting,duct |
| R2215 |
T10376 |
T10366 |
punct |
", ",turned |
| R2216 |
T10377 |
T10366 |
nsubj |
we,turned |
| R2217 |
T10225 |
T10226 |
compound |
duct,cells |
| R2218 |
T10378 |
T10366 |
prep |
to,turned |
| R2219 |
T10379 |
T10378 |
pobj |
transfection,to |
| R2220 |
T10226 |
T10223 |
pobj |
cells,of |
| R2221 |
T10380 |
T10379 |
prep |
of,transfection |
| R2222 |
T10381 |
T10382 |
compound |
MDCK,cells |
| R2223 |
T10382 |
T10380 |
pobj |
cells,of |
| R2224 |
T10227 |
T10221 |
prep |
in,region |
| R2225 |
T10383 |
T10366 |
punct |
.,turned |
| R2226 |
T10228 |
T10227 |
pobj |
kidneys,in |
| R2227 |
T10385 |
T10386 |
amod |
Stable,lines |
| R2228 |
T10386 |
T10388 |
nsubjpass |
lines,established |
| R2229 |
T10387 |
T10386 |
compound |
cell,lines |
| R2230 |
T10229 |
T10228 |
prep |
of,kidneys |
| R2231 |
T10389 |
T10386 |
acl |
expressing,lines |
| R2232 |
T10390 |
T10391 |
nmod |
mouse,AQP2 |
| R2233 |
T10230 |
T10231 |
amod |
wild,type |
| R2234 |
T10391 |
T10389 |
dobj |
AQP2,expressing |
| R2235 |
T10392 |
T10393 |
amod |
wild,type |
| R2236 |
T10393 |
T10391 |
compound |
type,AQP2 |
| R2237 |
T10231 |
T10233 |
compound |
type,mice |
| R2238 |
T10394 |
T10393 |
punct |
-,type |
| R2239 |
T10395 |
T10391 |
cc |
and,AQP2 |
| R2240 |
T10232 |
T10231 |
punct |
-,type |
| R2241 |
T10396 |
T10397 |
compound |
AQP2,F204V |
| R2242 |
T10397 |
T10391 |
conj |
F204V,AQP2 |
| R2243 |
T10398 |
T10397 |
punct |
-,F204V |
| R2244 |
T10233 |
T10229 |
pobj |
mice,of |
| R2245 |
T10399 |
T10388 |
auxpass |
were,established |
| R2246 |
T10400 |
T10388 |
punct |
.,established |
| R2247 |
T10234 |
T10202 |
punct |
.,localized |
| R2248 |
T10402 |
T10403 |
nsubj |
Immunoblots,showed |
| R2249 |
T10236 |
T10237 |
prep |
Upon,translocated |
| R2250 |
T10404 |
T10402 |
prep |
of,Immunoblots |
| R2251 |
T10405 |
T10406 |
compound |
protein,extracts |
| R2252 |
T10406 |
T10404 |
pobj |
extracts,of |
| R2253 |
T10407 |
T10406 |
prep |
from,extracts |
| R2254 |
T10408 |
T10409 |
amod |
stable,lines |
| R2255 |
T10409 |
T10407 |
pobj |
lines,from |
| R2256 |
T10410 |
T10409 |
compound |
cell,lines |
| R2257 |
T10238 |
T10236 |
pobj |
stimulation,Upon |
| R2258 |
T10411 |
T10412 |
mark |
that,recapitulate |
| R2259 |
T10412 |
T10403 |
ccomp |
recapitulate,showed |
| R2260 |
T10413 |
T10414 |
compound |
MDCK,cells |
| R2261 |
T10414 |
T10412 |
nsubj |
cells,recapitulate |
| R2262 |
T10415 |
T10416 |
det |
the,defect |
| R2263 |
T10239 |
T10238 |
prep |
with,stimulation |
| R2264 |
T10416 |
T10412 |
dobj |
defect,recapitulate |
| R2265 |
T10417 |
T10416 |
compound |
glycosylation,defect |
| R2266 |
T10240 |
T10239 |
pobj |
dDAVP,with |
| R2267 |
T10418 |
T10416 |
acl |
seen,defect |
| R2268 |
T10419 |
T10418 |
prep |
in,seen |
| R2269 |
T10420 |
T10421 |
compound |
mutant,mice |
| R2270 |
T10241 |
T10237 |
punct |
", ",translocated |
| R2271 |
T10421 |
T10419 |
pobj |
mice,in |
| R2272 |
T10422 |
T10423 |
punct |
(,data |
| R2273 |
T10423 |
T10403 |
meta |
data,showed |
| R2274 |
T10424 |
T10423 |
amod |
unpublished,data |
| R2275 |
T10242 |
T10237 |
nsubj |
AQP2,translocated |
| R2276 |
T10425 |
T10423 |
punct |
),data |
| R2277 |
T10426 |
T10403 |
punct |
.,showed |
| R2278 |
T10243 |
T10237 |
prep |
to,translocated |
| R2279 |
T10428 |
T10429 |
det |
The,protein |
| R2280 |
T10429 |
T10433 |
nsubj |
protein,was |
| R2281 |
T10244 |
T10243 |
cc |
or,to |
| R2282 |
T10430 |
T10431 |
amod |
wild,type |
| R2283 |
T10431 |
T10429 |
compound |
type,protein |
| R2284 |
T10432 |
T10431 |
punct |
-,type |
| R2285 |
T10245 |
T10243 |
conj |
near,to |
| R2286 |
T10434 |
T10433 |
advmod |
again,was |
| R2287 |
T10246 |
T10247 |
det |
the,surface |
| R2288 |
T10435 |
T10433 |
acomp |
present,was |
| R2289 |
T10436 |
T10433 |
prep |
in,was |
| R2290 |
T10437 |
T10438 |
nummod |
three,forms |
| R2291 |
T10247 |
T10245 |
pobj |
surface,near |
| R2292 |
T10438 |
T10436 |
pobj |
forms,in |
| R2293 |
T10439 |
T10438 |
amod |
different,forms |
| R2294 |
T10248 |
T10247 |
compound |
cell,surface |
| R2295 |
T10440 |
T10433 |
punct |
.,was |
| R2296 |
T10442 |
T10443 |
nsubj |
Cells,lacked |
| R2297 |
T10249 |
T10250 |
punct |
(,row |
| R2298 |
T10444 |
T10442 |
acl |
expressing,Cells |
| R2299 |
T10445 |
T10446 |
compound |
AQP2,F204V |
| R2300 |
T10462 |
T10461 |
punct |
-,glycosylated |
| R2301 |
T10446 |
T10444 |
dobj |
F204V,expressing |
| R2302 |
T10447 |
T10446 |
punct |
-,F204V |
| R2303 |
T10448 |
T10449 |
det |
the,form |
| R2304 |
T10449 |
T10443 |
dobj |
form,lacked |
| R2305 |
T10463 |
T10464 |
nummod |
31,kDa |
| R2306 |
T10450 |
T10451 |
quantmod |
35,45 |
| R2307 |
T10451 |
T10453 |
nummod |
45,kDa |
| R2308 |
T10452 |
T10451 |
punct |
–,45 |
| R2309 |
T10453 |
T10449 |
compound |
kDa,form |
| R2310 |
T10454 |
T10443 |
cc |
and,lacked |
| R2311 |
T10455 |
T10456 |
auxpass |
were,enriched |
| R2312 |
T10464 |
T10459 |
compound |
kDa,form |
| R2313 |
T10456 |
T10443 |
conj |
enriched,lacked |
| R2314 |
T10457 |
T10456 |
prep |
in,enriched |
| R2315 |
T10465 |
T10443 |
punct |
.,lacked |
| R2316 |
T10458 |
T10459 |
det |
the,form |
| R2317 |
T10467 |
T10468 |
prep |
In,appeared |
| R2318 |
T10459 |
T10457 |
pobj |
form,in |
| R2319 |
T10460 |
T10461 |
npadvmod |
core,glycosylated |
| R2320 |
T10461 |
T10459 |
amod |
glycosylated,form |
| R2321 |
T10469 |
T10470 |
amod |
transfected,cells |
| R2322 |
T10470 |
T10467 |
pobj |
cells,In |
| R2323 |
T10471 |
T10470 |
punct |
", ",cells |
| R2324 |
T10567 |
T10568 |
prep |
Along,seen |
| R2325 |
T10472 |
T10470 |
amod |
unstimulated,cells |
| R2326 |
T10569 |
T10570 |
det |
the,axis |
| R2327 |
T10570 |
T10567 |
pobj |
axis,Along |
| R2328 |
T10473 |
T10470 |
compound |
MDCK,cells |
| R2329 |
T10571 |
T10570 |
compound |
z,axis |
| R2330 |
T10572 |
T10570 |
punct |
-,axis |
| R2331 |
T10573 |
T10568 |
punct |
", ",seen |
| R2332 |
T10474 |
T10468 |
punct |
", ",appeared |
| R2333 |
T10574 |
T10575 |
det |
the,distribution |
| R2334 |
T10475 |
T10476 |
amod |
wild,type |
| R2335 |
T10476 |
T10478 |
compound |
type,AQP2 |
| R2336 |
T10575 |
T10568 |
nsubjpass |
distribution,seen |
| R2337 |
T10576 |
T10575 |
amod |
perinuclear,distribution |
| R2338 |
T10477 |
T10476 |
punct |
-,type |
| R2339 |
T10577 |
T10575 |
prep |
of,distribution |
| R2340 |
T10578 |
T10579 |
compound |
AQP2,F204V |
| R2341 |
T10478 |
T10468 |
nsubj |
AQP2,appeared |
| R2342 |
T10579 |
T10577 |
pobj |
F204V,of |
| R2343 |
T10580 |
T10579 |
punct |
-,F204V |
| R2344 |
T10581 |
T10568 |
auxpass |
was,seen |
| R2345 |
T10582 |
T10568 |
advmod |
clearly,seen |
| R2346 |
T10479 |
T10478 |
punct |
(,AQP2 |
| R2347 |
T10583 |
T10568 |
punct |
", ",seen |
| R2348 |
T10584 |
T10568 |
cc |
and,seen |
| R2349 |
T10585 |
T10586 |
det |
this,distribution |
| R2350 |
T10480 |
T10478 |
acl |
stained,AQP2 |
| R2351 |
T10586 |
T10587 |
nsubjpass |
distribution,altered |
| R2352 |
T10587 |
T10568 |
conj |
altered,seen |
| R2353 |
T10588 |
T10587 |
auxpass |
is,altered |
| R2354 |
T10481 |
T10480 |
oprd |
green,stained |
| R2355 |
T10589 |
T10587 |
neg |
not,altered |
| R2356 |
T10590 |
T10587 |
agent |
by,altered |
| R2357 |
T10482 |
T10478 |
punct |
),AQP2 |
| R2358 |
T10591 |
T10590 |
pobj |
forskolin,by |
| R2359 |
T10592 |
T10593 |
punct |
(,columns |
| R2360 |
T10593 |
T10587 |
parataxis |
columns,altered |
| R2361 |
T10594 |
T10593 |
dep |
Figure,columns |
| R2362 |
T10595 |
T10594 |
nummod |
4B,Figure |
| R2363 |
T10596 |
T10593 |
punct |
", ",columns |
| R2364 |
T10483 |
T10468 |
prep |
in,appeared |
| R2365 |
T10597 |
T10598 |
amod |
bottom,row |
| R2366 |
T10598 |
T10593 |
dep |
row,columns |
| R2367 |
T10599 |
T10593 |
punct |
", ",columns |
| R2368 |
T10484 |
T10485 |
det |
a,pattern |
| R2369 |
T10600 |
T10593 |
nummod |
two,columns |
| R2370 |
T10601 |
T10593 |
amod |
right,columns |
| R2371 |
T10602 |
T10593 |
punct |
),columns |
| R2372 |
T10485 |
T10483 |
pobj |
pattern,in |
| R2373 |
T10603 |
T10587 |
punct |
.,altered |
| R2374 |
T10486 |
T10485 |
compound |
punctate,pattern |
| R2375 |
T10605 |
T10606 |
det |
The,distribution |
| R2376 |
T10606 |
T10608 |
nsubj |
distribution,is |
| R2377 |
T10607 |
T10606 |
amod |
perinuclear,distribution |
| R2378 |
T10487 |
T10485 |
acl |
distributed,pattern |
| R2379 |
T10609 |
T10606 |
prep |
of,distribution |
| R2380 |
T10610 |
T10611 |
compound |
AQP2,F204V |
| R2381 |
T10488 |
T10487 |
prep |
throughout,distributed |
| R2382 |
T10611 |
T10609 |
pobj |
F204V,of |
| R2383 |
T10612 |
T10611 |
punct |
-,F204V |
| R2384 |
T10489 |
T10490 |
det |
the,region |
| R2385 |
T10613 |
T10608 |
acomp |
consistent,is |
| R2386 |
T10614 |
T10613 |
prep |
with,consistent |
| R2387 |
T10615 |
T10616 |
det |
an,compartmentalization |
| R2388 |
T10616 |
T10614 |
pobj |
compartmentalization,with |
| R2389 |
T10490 |
T10488 |
pobj |
region,throughout |
| R2390 |
T10617 |
T10616 |
compound |
ER,compartmentalization |
| R2391 |
T10618 |
T10608 |
punct |
.,is |
| R2392 |
T10491 |
T10490 |
amod |
subapical,region |
| R2393 |
T10620 |
T10621 |
aux |
To,test |
| R2394 |
T10621 |
T10622 |
advcl |
test,co-stained |
| R2395 |
T10492 |
T10493 |
punct |
(,photomicrographs |
| R2396 |
T10623 |
T10624 |
det |
the,idea |
| R2397 |
T10493 |
T10468 |
parataxis |
photomicrographs,appeared |
| R2398 |
T10624 |
T10621 |
dobj |
idea,test |
| R2399 |
T10625 |
T10626 |
mark |
that,localizes |
| R2400 |
T10626 |
T10624 |
acl |
localizes,idea |
| R2401 |
T10494 |
T10493 |
dep |
Figure,photomicrographs |
| R2402 |
T10627 |
T10628 |
compound |
AQP2,F204V |
| R2403 |
T10628 |
T10626 |
nsubj |
F204V,localizes |
| R2404 |
T10629 |
T10628 |
punct |
-,F204V |
| R2405 |
T10495 |
T10494 |
nummod |
4B,Figure |
| R2406 |
T10630 |
T10626 |
prep |
to,localizes |
| R2407 |
T10631 |
T10632 |
det |
the,ER |
| R2408 |
T10496 |
T10493 |
punct |
", ",photomicrographs |
| R2409 |
T10497 |
T10498 |
amod |
left,column |
| R2410 |
T10632 |
T10630 |
pobj |
ER,to |
| R2411 |
T10633 |
T10622 |
punct |
", ",co-stained |
| R2412 |
T10634 |
T10622 |
nsubj |
we,co-stained |
| R2413 |
T10635 |
T10622 |
dobj |
cells,co-stained |
| R2414 |
T10636 |
T10635 |
acl |
transfected,cells |
| R2415 |
T10498 |
T10493 |
compound |
column,photomicrographs |
| R2416 |
T10637 |
T10636 |
prep |
with,transfected |
| R2417 |
T10638 |
T10637 |
pobj |
Aqp2F204V,with |
| R2418 |
T10499 |
T10493 |
punct |
),photomicrographs |
| R2419 |
T10639 |
T10640 |
punct |
(,cDNA |
| R2420 |
T10640 |
T10638 |
parataxis |
cDNA,Aqp2F204V |
| R2421 |
T10500 |
T10468 |
punct |
", ",appeared |
| R2422 |
T10641 |
T10640 |
punct |
),cDNA |
| R2423 |
T10642 |
T10622 |
prep |
for,co-stained |
| R2424 |
T10643 |
T10642 |
pobj |
AQP2,for |
| R2425 |
T10501 |
T10468 |
advcl |
consistent,appeared |
| R2426 |
T10644 |
T10643 |
cc |
and,AQP2 |
| R2427 |
T10645 |
T10646 |
det |
an,marker |
| R2428 |
T10646 |
T10643 |
conj |
marker,AQP2 |
| R2429 |
T10502 |
T10501 |
prep |
with,consistent |
| R2430 |
T10647 |
T10646 |
compound |
ER,marker |
| R2431 |
T10648 |
T10646 |
punct |
", ",marker |
| R2432 |
T10649 |
T10646 |
appos |
calnexin,marker |
| R2433 |
T10503 |
T10504 |
amod |
vesicular,compartmentalization |
| R2434 |
T10650 |
T10651 |
punct |
(,Figure |
| R2435 |
T10651 |
T10622 |
parataxis |
Figure,co-stained |
| R2436 |
T10652 |
T10651 |
nummod |
4C,Figure |
| R2437 |
T10504 |
T10502 |
pobj |
compartmentalization,with |
| R2438 |
T10653 |
T10651 |
punct |
),Figure |
| R2439 |
T10654 |
T10622 |
punct |
.,co-stained |
| R2440 |
T10505 |
T10468 |
punct |
.,appeared |
| R2441 |
T10656 |
T10657 |
nsubjpass |
Colocalization,investigated |
| R2442 |
T10658 |
T10656 |
prep |
of,Colocalization |
| R2443 |
T10507 |
T10508 |
compound |
AQP2,F204V |
| R2444 |
T10659 |
T10658 |
pobj |
calnexin,of |
| R2445 |
T10660 |
T10656 |
prep |
with,Colocalization |
| R2446 |
T10661 |
T10660 |
pobj |
AQP2,with |
| R2447 |
T10662 |
T10657 |
auxpass |
was,investigated |
| R2448 |
T10508 |
T10510 |
nsubj |
F204V,appeared |
| R2449 |
T10663 |
T10657 |
advmod |
directly,investigated |
| R2450 |
T10664 |
T10657 |
punct |
", ",investigated |
| R2451 |
T10665 |
T10657 |
cc |
and,investigated |
| R2452 |
T10509 |
T10508 |
punct |
-,F204V |
| R2453 |
T10666 |
T10667 |
nsubjpass |
it,found |
| R2454 |
T10667 |
T10657 |
conj |
found,investigated |
| R2455 |
T10668 |
T10667 |
auxpass |
was,found |
| R2456 |
T10511 |
T10510 |
punct |
", ",appeared |
| R2457 |
T10669 |
T10670 |
mark |
that,colocalized |
| R2458 |
T10670 |
T10667 |
ccomp |
colocalized,found |
| R2459 |
T10671 |
T10672 |
nummod |
80,% |
| R2460 |
T10672 |
T10670 |
nsubj |
%,colocalized |
| R2461 |
T10512 |
T10510 |
prep |
on,appeared |
| R2462 |
T10513 |
T10514 |
det |
the,hand |
| R2463 |
T10673 |
T10672 |
prep |
of,% |
| R2464 |
T10514 |
T10512 |
pobj |
hand,on |
| R2465 |
T10674 |
T10675 |
det |
all,protein |
| R2466 |
T10675 |
T10673 |
pobj |
protein,of |
| R2467 |
T10676 |
T10677 |
compound |
AQP2,F204V |
| R2468 |
T10515 |
T10514 |
amod |
other,hand |
| R2469 |
T10677 |
T10675 |
compound |
F204V,protein |
| R2470 |
T10678 |
T10677 |
punct |
-,F204V |
| R2471 |
T10679 |
T10670 |
prep |
with,colocalized |
| R2472 |
T10516 |
T10510 |
punct |
", ",appeared |
| R2473 |
T10680 |
T10679 |
pobj |
calnexin,with |
| R2474 |
T10681 |
T10667 |
punct |
.,found |
| R2475 |
T10517 |
T10510 |
prep |
in,appeared |
| R2476 |
T10683 |
T10684 |
det |
The,% |
| R2477 |
T10684 |
T10687 |
nsubj |
%,appeared |
| R2478 |
T10518 |
T10519 |
det |
a,pattern |
| R2479 |
T10685 |
T10684 |
amod |
remaining,% |
| R2480 |
T10686 |
T10684 |
nummod |
20,% |
| R2481 |
T10519 |
T10517 |
pobj |
pattern,in |
| R2482 |
T10688 |
T10687 |
prep |
at,appeared |
| R2483 |
T10689 |
T10690 |
det |
the,periphery |
| R2484 |
T10520 |
T10521 |
npadvmod |
punctate,perinuclear |
| R2485 |
T10690 |
T10688 |
pobj |
periphery,at |
| R2486 |
T10691 |
T10690 |
prep |
of,periphery |
| R2487 |
T10692 |
T10693 |
det |
the,ER |
| R2488 |
T10521 |
T10519 |
amod |
perinuclear,pattern |
| R2489 |
T10693 |
T10691 |
pobj |
ER,of |
| R2490 |
T10694 |
T10687 |
punct |
", ",appeared |
| R2491 |
T10695 |
T10687 |
advcl |
representing,appeared |
| R2492 |
T10522 |
T10521 |
cc |
but,perinuclear |
| R2493 |
T10696 |
T10697 |
compound |
AQP2,F204V |
| R2494 |
T10697 |
T10695 |
dobj |
F204V,representing |
| R2495 |
T10698 |
T10697 |
punct |
-,F204V |
| R2496 |
T10523 |
T10524 |
punct |
(,column |
| R2497 |
T10699 |
T10700 |
dep |
that,progressed |
| R2498 |
T10700 |
T10697 |
relcl |
progressed,F204V |
| R2499 |
T10524 |
T10510 |
parataxis |
column,appeared |
| R2500 |
T10701 |
T10700 |
aux |
had,progressed |
| R2501 |
T10702 |
T10700 |
advmod |
potentially,progressed |
| R2502 |
T10703 |
T10700 |
prep |
beyond,progressed |
| R2503 |
T10525 |
T10524 |
dep |
Figure,column |
| R2504 |
T10704 |
T10705 |
det |
the,ER |
| R2505 |
T10705 |
T10703 |
pobj |
ER,beyond |
| R2506 |
T10706 |
T10687 |
punct |
.,appeared |
| R2507 |
T10526 |
T10525 |
nummod |
4B,Figure |
| R2508 |
T10708 |
T10709 |
det |
This,escape |
| R2509 |
T10709 |
T10712 |
nsubj |
escape,was |
| R2510 |
T10527 |
T10524 |
punct |
", ",column |
| R2511 |
T10710 |
T10709 |
punct |
“,escape |
| R2512 |
T10711 |
T10709 |
compound |
ER,escape |
| R2513 |
T10528 |
T10524 |
amod |
third,column |
| R2514 |
T10713 |
T10709 |
punct |
”,escape |
| R2515 |
T10714 |
T10712 |
acomp |
consistent,was |
| R2516 |
T10715 |
T10714 |
prep |
with,consistent |
| R2517 |
T10716 |
T10717 |
det |
the,proportion |
| R2518 |
T10717 |
T10715 |
pobj |
proportion,with |
| R2519 |
T10529 |
T10524 |
punct |
),column |
| R2520 |
T10718 |
T10717 |
amod |
small,proportion |
| R2521 |
T10530 |
T10510 |
punct |
.,appeared |
| R2522 |
T10719 |
T10717 |
prep |
of,proportion |
| R2523 |
T10720 |
T10721 |
amod |
mature,F204V |
| R2524 |
T10721 |
T10719 |
pobj |
F204V,of |
| R2525 |
T10532 |
T10533 |
prep |
Upon,translocated |
| R2526 |
T10722 |
T10721 |
punct |
", ",F204V |
| R2527 |
T10723 |
T10724 |
amod |
complex,glycosylated |
| R2528 |
T10724 |
T10721 |
amod |
glycosylated,F204V |
| R2529 |
T10725 |
T10721 |
punct |
", ",F204V |
| R2530 |
T10534 |
T10532 |
pobj |
stimulation,Upon |
| R2531 |
T10726 |
T10721 |
compound |
AQP2,F204V |
| R2532 |
T10727 |
T10721 |
punct |
-,F204V |
| R2533 |
T10728 |
T10717 |
prep |
in,proportion |
| R2534 |
T10535 |
T10534 |
prep |
with,stimulation |
| R2535 |
T10729 |
T10730 |
compound |
mutant,kidneys |
| R2536 |
T10730 |
T10728 |
pobj |
kidneys,in |
| R2537 |
T10731 |
T10732 |
punct |
(,see |
| R2538 |
T10536 |
T10535 |
pobj |
forskolin,with |
| R2539 |
T10732 |
T10712 |
parataxis |
see,was |
| R2540 |
T10733 |
T10732 |
dobj |
Figure,see |
| R2541 |
T10537 |
T10533 |
punct |
", ",translocated |
| R2542 |
T10734 |
T10733 |
nummod |
3A,Figure |
| R2543 |
T10735 |
T10732 |
punct |
),see |
| R2544 |
T10736 |
T10712 |
punct |
.,was |
| R2545 |
T10538 |
T10539 |
det |
a,activator |
| R2546 |
T10738 |
T10739 |
nsubjpass |
Animals,affected |
| R2547 |
T10539 |
T10533 |
nsubj |
activator,translocated |
| R2548 |
T10740 |
T10738 |
amod |
heterozygous,Animals |
| R2549 |
T10741 |
T10740 |
prep |
for,heterozygous |
| R2550 |
T10742 |
T10743 |
det |
the,mutation |
| R2551 |
T10540 |
T10541 |
npadvmod |
cAMP,dependent |
| R2552 |
T10743 |
T10741 |
pobj |
mutation,for |
| R2553 |
T10744 |
T10743 |
compound |
Aqp2F204V,mutation |
| R2554 |
T10745 |
T10739 |
auxpass |
were,affected |
| R2555 |
T10541 |
T10539 |
amod |
dependent,activator |
| R2556 |
T10746 |
T10739 |
neg |
not,affected |
| R2557 |
T10747 |
T10739 |
prep |
in,affected |
| R2558 |
T10748 |
T10749 |
poss |
their,production |
| R2559 |
T10542 |
T10541 |
punct |
-,dependent |
| R2560 |
T10749 |
T10747 |
pobj |
production,in |
| R2561 |
T10750 |
T10749 |
compound |
urine,production |
| R2562 |
T10751 |
T10749 |
cc |
or,production |
| R2563 |
T10543 |
T10544 |
compound |
protein,kinase |
| R2564 |
T10752 |
T10753 |
compound |
urine,osmolality |
| R2565 |
T10753 |
T10749 |
conj |
osmolality,production |
| R2566 |
T10754 |
T10755 |
punct |
(,see |
| R2567 |
T10755 |
T10739 |
parataxis |
see,affected |
| R2568 |
T10756 |
T10757 |
nmod |
Figure,1C |
| R2569 |
T10757 |
T10755 |
dobj |
1C,see |
| R2570 |
T10544 |
T10539 |
compound |
kinase,activator |
| R2571 |
T10758 |
T10757 |
cc |
and,1C |
| R2572 |
T10759 |
T10757 |
conj |
1D,1C |
| R2573 |
T10760 |
T10755 |
punct |
),see |
| R2574 |
T10545 |
T10539 |
punct |
", ",activator |
| R2575 |
T10761 |
T10739 |
punct |
.,affected |
| R2576 |
T10763 |
T10764 |
nsubjpass |
It,shown |
| R2577 |
T10546 |
T10547 |
amod |
wild,type |
| R2578 |
T10765 |
T10764 |
aux |
has,shown |
| R2579 |
T10766 |
T10764 |
advmod |
also,shown |
| R2580 |
T10547 |
T10549 |
compound |
type,AQP2 |
| R2581 |
T10767 |
T10764 |
auxpass |
been,shown |
| R2582 |
T10768 |
T10769 |
mark |
that,interact |
| R2583 |
T10769 |
T10764 |
ccomp |
interact,shown |
| R2584 |
T10548 |
T10547 |
punct |
-,type |
| R2585 |
T10770 |
T10771 |
det |
a,allele |
| R2586 |
T10771 |
T10769 |
nsubj |
allele,interact |
| R2587 |
T10772 |
T10771 |
amod |
recessive,allele |
| R2588 |
T10549 |
T10539 |
appos |
AQP2,activator |
| R2589 |
T10773 |
T10771 |
compound |
NDI,allele |
| R2590 |
T10774 |
T10771 |
punct |
", ",allele |
| R2591 |
T10775 |
T10776 |
compound |
AQP2,R187C |
| R2592 |
T10550 |
T10533 |
prep |
to,translocated |
| R2593 |
T10776 |
T10771 |
appos |
R187C,allele |
| R2594 |
T10777 |
T10776 |
punct |
-,R187C |
| R2595 |
T10778 |
T10769 |
punct |
", ",interact |
| R2596 |
T10551 |
T10552 |
det |
the,surface |
| R2597 |
T10552 |
T10550 |
pobj |
surface,to |
| R2598 |
T10553 |
T10552 |
amod |
apical,surface |
| R2599 |
T10779 |
T10769 |
aux |
does,interact |
| R2600 |
T10554 |
T10552 |
prep |
of,surface |
| R2601 |
T10780 |
T10769 |
neg |
not,interact |
| R2602 |
T10781 |
T10769 |
prep |
with,interact |
| R2603 |
T10782 |
T10783 |
amod |
wild,type |
| R2604 |
T10555 |
T10556 |
amod |
polarized,cells |
| R2605 |
T10783 |
T10785 |
compound |
type,protein |
| R2606 |
T10784 |
T10783 |
punct |
-,type |
| R2607 |
T10556 |
T10554 |
pobj |
cells,of |
| R2608 |
T10785 |
T10781 |
pobj |
protein,with |
| R2609 |
T10786 |
T10769 |
prep |
in,interact |
| R2610 |
T10787 |
T10786 |
pobj |
oocytes,in |
| R2611 |
T10557 |
T10556 |
compound |
MDCK,cells |
| R2612 |
T10788 |
T10789 |
punct |
[,24 |
| R2613 |
T10789 |
T10769 |
parataxis |
24,interact |
| R2614 |
T10790 |
T10789 |
punct |
],24 |
| R2615 |
T10558 |
T10559 |
punct |
(,column |
| R2616 |
T10791 |
T10769 |
punct |
", ",interact |
| R2617 |
T10792 |
T10769 |
cc |
nor,interact |
| R2618 |
T10793 |
T10794 |
aux |
does,homo-oligomerize |
| R2619 |
T10794 |
T10769 |
conj |
homo-oligomerize,interact |
| R2620 |
T10795 |
T10794 |
nsubj |
it,homo-oligomerize |
| R2621 |
T10796 |
T10794 |
prep |
in,homo-oligomerize |
| R2622 |
T10559 |
T10533 |
parataxis |
column,translocated |
| R2623 |
T10797 |
T10798 |
compound |
MDCK,cells |
| R2624 |
T10798 |
T10796 |
pobj |
cells,in |
| R2625 |
T10799 |
T10800 |
punct |
[,22 |
| R2626 |
T10560 |
T10559 |
dep |
Figure,column |
| R2627 |
T10800 |
T10764 |
parataxis |
22,shown |
| R2628 |
T10801 |
T10800 |
punct |
],22 |
| R2629 |
T10802 |
T10764 |
punct |
.,shown |
| R2630 |
T10561 |
T10560 |
nummod |
4B,Figure |
| R2631 |
T10804 |
T10805 |
advmod |
Therefore,examined |
| R2632 |
T10562 |
T10559 |
punct |
", ",column |
| R2633 |
T10563 |
T10559 |
amod |
second,column |
| R2634 |
T10806 |
T10805 |
punct |
", ",examined |
| R2635 |
T10564 |
T10559 |
punct |
),column |
| R2636 |
T10807 |
T10805 |
nsubjpass |
kidneys,examined |
| R2637 |
T10808 |
T10807 |
prep |
from,kidneys |
| R2638 |
T10565 |
T10533 |
punct |
.,translocated |
| R2639 |
T10809 |
T10810 |
amod |
heterozygous,animals |
| R2640 |
T10810 |
T10808 |
pobj |
animals,from |
| R2641 |
T10811 |
T10805 |
auxpass |
were,examined |
| R2642 |
T10885 |
T10884 |
prep |
by,undetectable |
| R2643 |
T10812 |
T10805 |
prep |
for,examined |
| R2644 |
T10813 |
T10812 |
pobj |
evidence,for |
| R2645 |
T10814 |
T10813 |
prep |
of,evidence |
| R2646 |
T10815 |
T10816 |
nummod |
two,populations |
| R2647 |
T10886 |
T10885 |
pobj |
immunocytochemistry,by |
| R2648 |
T10816 |
T10814 |
pobj |
populations,of |
| R2649 |
T10817 |
T10816 |
prep |
of,populations |
| R2650 |
T10818 |
T10819 |
compound |
AQP2,protein |
| R2651 |
T10887 |
T10868 |
punct |
.,reflect |
| R2652 |
T10819 |
T10817 |
pobj |
protein,of |
| R2653 |
T10820 |
T10805 |
punct |
.,examined |
| R2654 |
T10889 |
T10890 |
advmod |
Alternatively,alter |
| R2655 |
T10822 |
T10823 |
advmod |
Surprisingly,revealed |
| R2656 |
T10824 |
T10823 |
punct |
", ",revealed |
| R2657 |
T10891 |
T10890 |
punct |
", ",alter |
| R2658 |
T10825 |
T10826 |
amod |
immunohistochemical,staining |
| R2659 |
T10826 |
T10823 |
nsubj |
staining,revealed |
| R2660 |
T10827 |
T10826 |
prep |
of,staining |
| R2661 |
T10828 |
T10829 |
nmod |
kidney,ducts |
| R2662 |
T10892 |
T10893 |
det |
the,presence |
| R2663 |
T10829 |
T10827 |
pobj |
ducts,of |
| R2664 |
T10830 |
T10829 |
amod |
collecting,ducts |
| R2665 |
T10831 |
T10829 |
prep |
from,ducts |
| R2666 |
T10893 |
T10890 |
nsubj |
presence,alter |
| R2667 |
T10832 |
T10833 |
nmod |
Aqp2F204V,mice |
| R2668 |
T10833 |
T10831 |
pobj |
mice,from |
| R2669 |
T10834 |
T10832 |
punct |
/,Aqp2F204V |
| R2670 |
T10835 |
T10832 |
punct |
+,Aqp2F204V |
| R2671 |
T10836 |
T10837 |
det |
a,pattern |
| R2672 |
T10837 |
T10823 |
dobj |
pattern,revealed |
| R2673 |
T10894 |
T10893 |
prep |
of,presence |
| R2674 |
T10838 |
T10839 |
advmod |
remarkably,similar |
| R2675 |
T10839 |
T10837 |
amod |
similar,pattern |
| R2676 |
T10840 |
T10839 |
prep |
to,similar |
| R2677 |
T10895 |
T10896 |
amod |
wild,type |
| R2678 |
T10841 |
T10842 |
amod |
wild,type |
| R2679 |
T10842 |
T10840 |
pobj |
type,to |
| R2680 |
T10843 |
T10844 |
punct |
(,Figure |
| R2681 |
T10844 |
T10823 |
parataxis |
Figure,revealed |
| R2682 |
T10845 |
T10844 |
nummod |
5A,Figure |
| R2683 |
T10846 |
T10844 |
punct |
),Figure |
| R2684 |
T10847 |
T10823 |
punct |
.,revealed |
| R2685 |
T10896 |
T10898 |
compound |
type,protein |
| R2686 |
T10849 |
T10850 |
nsubj |
AQP2,translocated |
| R2687 |
T10897 |
T10896 |
punct |
-,type |
| R2688 |
T10851 |
T10850 |
advmod |
completely,translocated |
| R2689 |
T10852 |
T10850 |
prep |
to,translocated |
| R2690 |
T10898 |
T10894 |
pobj |
protein,of |
| R2691 |
T10853 |
T10854 |
det |
the,surface |
| R2692 |
T10854 |
T10852 |
pobj |
surface,to |
| R2693 |
T10855 |
T10854 |
amod |
apical,surface |
| R2694 |
T10856 |
T10854 |
compound |
cell,surface |
| R2695 |
T10899 |
T10890 |
aux |
may,alter |
| R2696 |
T10857 |
T10850 |
prep |
upon,translocated |
| R2697 |
T10858 |
T10859 |
compound |
dDAVP,stimulation |
| R2698 |
T10859 |
T10857 |
pobj |
stimulation,upon |
| R2699 |
T10900 |
T10901 |
det |
the,localization |
| R2700 |
T10860 |
T10850 |
punct |
.,translocated |
| R2701 |
T10901 |
T10890 |
dobj |
localization,alter |
| R2702 |
T10862 |
T10863 |
det |
This,pattern |
| R2703 |
T10863 |
T10868 |
nsubj |
pattern,reflect |
| R2704 |
T10864 |
T10865 |
amod |
wild,type |
| R2705 |
T10902 |
T10901 |
prep |
of,localization |
| R2706 |
T10865 |
T10863 |
compound |
type,pattern |
| R2707 |
T10866 |
T10865 |
punct |
-,type |
| R2708 |
T10867 |
T10863 |
compound |
staining,pattern |
| R2709 |
T10903 |
T10904 |
det |
the,protein |
| R2710 |
T10869 |
T10868 |
aux |
may,reflect |
| R2711 |
T10904 |
T10902 |
pobj |
protein,of |
| R2712 |
T10870 |
T10868 |
advmod |
simply,reflect |
| R2713 |
T10871 |
T10872 |
det |
the,fact |
| R2714 |
T10872 |
T10868 |
dobj |
fact,reflect |
| R2715 |
T10873 |
T10874 |
mark |
that,makes |
| R2716 |
T10905 |
T10904 |
compound |
mutant,protein |
| R2717 |
T10874 |
T10872 |
acl |
makes,fact |
| R2718 |
T10875 |
T10874 |
csubj |
decreasing,makes |
| R2719 |
T10876 |
T10877 |
det |
the,amount |
| R2720 |
T10906 |
T10890 |
punct |
.,alter |
| R2721 |
T10877 |
T10875 |
dobj |
amount,decreasing |
| R2722 |
T10878 |
T10877 |
prep |
of,amount |
| R2723 |
T10879 |
T10880 |
compound |
mutant,protein |
| R2724 |
T10880 |
T10878 |
pobj |
protein,of |
| R2725 |
T10881 |
T10875 |
prep |
by,decreasing |
| R2726 |
T10882 |
T10881 |
pobj |
half,by |
| R2727 |
T10908 |
T10909 |
advmod |
Indeed,proposed |
| R2728 |
T10883 |
T10884 |
nsubj |
it,undetectable |
| R2729 |
T10884 |
T10874 |
ccomp |
undetectable,makes |
| R2730 |
T10910 |
T10909 |
punct |
", ",proposed |
| R2731 |
T10911 |
T10909 |
nsubj |
Hendriks,proposed |
| R2732 |
T10912 |
T10913 |
advmod |
et,al. |
| R2733 |
T10913 |
T10911 |
advmod |
al.,Hendriks |
| R2734 |
T10991 |
T10980 |
nsubj |
type,rescue |
| R2735 |
T10914 |
T10915 |
det |
a,mechanism |
| R2736 |
T10992 |
T10991 |
amod |
wild,type |
| R2737 |
T10915 |
T10909 |
dobj |
mechanism,proposed |
| R2738 |
T10993 |
T10980 |
aux |
may,rescue |
| R2739 |
T10994 |
T10995 |
det |
the,protein |
| R2740 |
T10916 |
T10915 |
punct |
“,mechanism |
| R2741 |
T10995 |
T10980 |
dobj |
protein,rescue |
| R2742 |
T10996 |
T10995 |
compound |
mutant,protein |
| R2743 |
T10997 |
T10998 |
mark |
as,suggested |
| R2744 |
T10917 |
T10918 |
nmod |
piggy,back |
| R2745 |
T10998 |
T10980 |
advcl |
suggested,rescue |
| R2746 |
T10999 |
T10998 |
agent |
by,suggested |
| R2747 |
T10918 |
T10915 |
nmod |
back,mechanism |
| R2748 |
T11000 |
T11001 |
det |
the,distribution |
| R2749 |
T11001 |
T10999 |
pobj |
distribution,by |
| R2750 |
T11002 |
T11001 |
amod |
subcellular,distribution |
| R2751 |
T11003 |
T11001 |
prep |
of,distribution |
| R2752 |
T10919 |
T10918 |
punct |
-,back |
| R2753 |
T11004 |
T11005 |
compound |
AQP2,protein |
| R2754 |
T11005 |
T11003 |
pobj |
protein,of |
| R2755 |
T10920 |
T10915 |
punct |
”,mechanism |
| R2756 |
T11006 |
T10980 |
punct |
.,rescue |
| R2757 |
T11008 |
T11009 |
aux |
To,test |
| R2758 |
T10921 |
T10922 |
aux |
to,explain |
| R2759 |
T11009 |
T11010 |
advcl |
test,looked |
| R2760 |
T10922 |
T10909 |
advcl |
explain,proposed |
| R2761 |
T11011 |
T11012 |
det |
this,idea |
| R2762 |
T11012 |
T11009 |
dobj |
idea,test |
| R2763 |
T11013 |
T11010 |
punct |
", ",looked |
| R2764 |
T11014 |
T11010 |
nsubj |
we,looked |
| R2765 |
T10923 |
T10924 |
det |
the,transport |
| R2766 |
T11015 |
T11010 |
advmod |
first,looked |
| R2767 |
T11016 |
T11010 |
prep |
for,looked |
| R2768 |
T10924 |
T10922 |
dobj |
transport,explain |
| R2769 |
T11017 |
T11018 |
det |
an,interaction |
| R2770 |
T11018 |
T11016 |
pobj |
interaction,for |
| R2771 |
T11019 |
T11018 |
prep |
between,interaction |
| R2772 |
T10925 |
T10924 |
prep |
of,transport |
| R2773 |
T11020 |
T11021 |
nmod |
mutant,proteins |
| R2774 |
T11021 |
T11019 |
pobj |
proteins,between |
| R2775 |
T11022 |
T11020 |
cc |
and,mutant |
| R2776 |
T11023 |
T11024 |
amod |
wild,type |
| R2777 |
T11024 |
T11020 |
conj |
type,mutant |
| R2778 |
T10926 |
T10927 |
amod |
nonglycosylated,subunits |
| R2779 |
T11025 |
T11024 |
punct |
-,type |
| R2780 |
T11026 |
T11010 |
prep |
in,looked |
| R2781 |
T11027 |
T11028 |
amod |
transfected,cells |
| R2782 |
T10927 |
T10925 |
pobj |
subunits,of |
| R2783 |
T11028 |
T11026 |
pobj |
cells,in |
| R2784 |
T11029 |
T11028 |
compound |
MDCK,cells |
| R2785 |
T11030 |
T11031 |
punct |
(,Figure |
| R2786 |
T10928 |
T10927 |
prep |
of,subunits |
| R2787 |
T11031 |
T11010 |
parataxis |
Figure,looked |
| R2788 |
T11032 |
T11031 |
nummod |
5B,Figure |
| R2789 |
T11033 |
T11031 |
punct |
),Figure |
| R2790 |
T11034 |
T11010 |
punct |
.,looked |
| R2791 |
T10929 |
T10928 |
pobj |
AQP2,of |
| R2792 |
T11036 |
T11037 |
compound |
MDCK,cells |
| R2793 |
T10930 |
T10924 |
prep |
to,transport |
| R2794 |
T11037 |
T11038 |
nsubjpass |
cells,transfected |
| R2795 |
T11039 |
T11040 |
advmod |
stably,expressing |
| R2796 |
T10931 |
T10932 |
det |
the,surface |
| R2797 |
T11040 |
T11037 |
acl |
expressing,cells |
| R2798 |
T11041 |
T11042 |
amod |
wild,type |
| R2799 |
T10932 |
T10930 |
pobj |
surface,to |
| R2800 |
T11042 |
T11044 |
compound |
type,AQP2 |
| R2801 |
T11043 |
T11042 |
punct |
-,type |
| R2802 |
T11044 |
T11040 |
dobj |
AQP2,expressing |
| R2803 |
T10933 |
T10932 |
compound |
cell,surface |
| R2804 |
T11045 |
T11038 |
auxpass |
were,transfected |
| R2805 |
T11046 |
T11038 |
advmod |
transiently,transfected |
| R2806 |
T11047 |
T11038 |
prep |
with,transfected |
| R2807 |
T10934 |
T10924 |
prep |
by,transport |
| R2808 |
T11048 |
T11049 |
compound |
GFP,constructs |
| R2809 |
T11049 |
T11047 |
pobj |
constructs,with |
| R2810 |
T11050 |
T11049 |
compound |
expression,constructs |
| R2811 |
T10935 |
T10936 |
amod |
glycosylated,subunits |
| R2812 |
T11051 |
T11049 |
acl |
encoding,constructs |
| R2813 |
T11052 |
T11053 |
npadvmod |
GFP,tagged |
| R2814 |
T10936 |
T10934 |
pobj |
subunits,by |
| R2815 |
T11053 |
T11055 |
amod |
tagged,AQP2 |
| R2816 |
T11054 |
T11053 |
punct |
-,tagged |
| R2817 |
T11055 |
T11051 |
dobj |
AQP2,encoding |
| R2818 |
T10937 |
T10938 |
punct |
[,22 |
| R2819 |
T11056 |
T11057 |
amod |
wild,type |
| R2820 |
T11057 |
T11055 |
compound |
type,AQP2 |
| R2821 |
T11058 |
T11057 |
punct |
-,type |
| R2822 |
T10938 |
T10922 |
parataxis |
22,explain |
| R2823 |
T11059 |
T11055 |
punct |
", ",AQP2 |
| R2824 |
T11060 |
T11061 |
compound |
AQP2,F204V |
| R2825 |
T11061 |
T11055 |
conj |
F204V,AQP2 |
| R2826 |
T11062 |
T11061 |
punct |
-,F204V |
| R2827 |
T11063 |
T11061 |
punct |
", ",F204V |
| R2828 |
T11064 |
T11061 |
cc |
or,F204V |
| R2829 |
T10939 |
T10938 |
punct |
],22 |
| R2830 |
T11065 |
T11061 |
conj |
GFP,F204V |
| R2831 |
T11066 |
T11065 |
advmod |
alone,GFP |
| R2832 |
T10940 |
T10909 |
punct |
.,proposed |
| R2833 |
T10942 |
T10943 |
nsubjpass |
It,shown |
| R2834 |
T11067 |
T11038 |
punct |
.,transfected |
| R2835 |
T10944 |
T10943 |
aux |
has,shown |
| R2836 |
T11069 |
T11070 |
nsubj |
Antibodies,coimmunoprecipitated |
| R2837 |
T11071 |
T11069 |
prep |
against,Antibodies |
| R2838 |
T11072 |
T11071 |
pobj |
GFP,against |
| R2839 |
T10945 |
T10943 |
advmod |
also,shown |
| R2840 |
T11073 |
T11074 |
amod |
wild,type |
| R2841 |
T11074 |
T11076 |
compound |
type,AQP2 |
| R2842 |
T10946 |
T10943 |
auxpass |
been,shown |
| R2843 |
T11075 |
T11074 |
punct |
-,type |
| R2844 |
T11076 |
T11070 |
dobj |
AQP2,coimmunoprecipitated |
| R2845 |
T11077 |
T11078 |
advmod |
when,transfected |
| R2846 |
T10947 |
T10948 |
mark |
that,rescue |
| R2847 |
T11078 |
T11070 |
advcl |
transfected,coimmunoprecipitated |
| R2848 |
T11079 |
T11080 |
compound |
AQP2,GFP |
| R2849 |
T10948 |
T10943 |
ccomp |
rescue,shown |
| R2850 |
T11080 |
T11078 |
nsubjpass |
GFP,transfected |
| R2851 |
T11081 |
T11080 |
punct |
-,GFP |
| R2852 |
T11082 |
T11080 |
cc |
or,GFP |
| R2853 |
T10949 |
T10950 |
amod |
wild,type |
| R2854 |
T11083 |
T11084 |
compound |
AQP2,GFP |
| R2855 |
T11084 |
T11080 |
conj |
GFP,GFP |
| R2856 |
T11085 |
T11084 |
punct |
-,GFP |
| R2857 |
T10950 |
T10952 |
compound |
type,protein |
| R2858 |
T11086 |
T11084 |
compound |
F204V,GFP |
| R2859 |
T11087 |
T11084 |
punct |
-,GFP |
| R2860 |
T10951 |
T10950 |
punct |
-,type |
| R2861 |
T11088 |
T11078 |
auxpass |
was,transfected |
| R2862 |
T11089 |
T11078 |
advmod |
transiently,transfected |
| R2863 |
T11090 |
T11078 |
punct |
", ",transfected |
| R2864 |
T10952 |
T10948 |
nsubj |
protein,rescue |
| R2865 |
T11091 |
T11078 |
cc |
but,transfected |
| R2866 |
T11092 |
T11091 |
neg |
not,but |
| R2867 |
T11093 |
T11094 |
advmod |
when,transfected |
| R2868 |
T10953 |
T10952 |
compound |
AQP2,protein |
| R2869 |
T11094 |
T11078 |
conj |
transfected,transfected |
| R2870 |
T11095 |
T11094 |
nsubjpass |
GFP,transfected |
| R2871 |
T10954 |
T10948 |
aux |
can,rescue |
| R2872 |
T11096 |
T11095 |
prep |
by,GFP |
| R2873 |
T10955 |
T10956 |
det |
a,protein |
| R2874 |
T10956 |
T10948 |
dobj |
protein,rescue |
| R2875 |
T10957 |
T10958 |
npadvmod |
translocation,defective |
| R2876 |
T11097 |
T11096 |
pobj |
itself,by |
| R2877 |
T11098 |
T11094 |
auxpass |
was,transfected |
| R2878 |
T11099 |
T11094 |
advmod |
transiently,transfected |
| R2879 |
T11100 |
T11094 |
prep |
into,transfected |
| R2880 |
T11101 |
T11102 |
compound |
MDCK,cells |
| R2881 |
T10958 |
T10956 |
amod |
defective,protein |
| R2882 |
T11102 |
T11100 |
pobj |
cells,into |
| R2883 |
T11103 |
T11104 |
advmod |
stably,expressing |
| R2884 |
T11104 |
T11102 |
acl |
expressing,cells |
| R2885 |
T10959 |
T10958 |
punct |
-,defective |
| R2886 |
T11105 |
T11106 |
amod |
wild,type |
| R2887 |
T11106 |
T11108 |
compound |
type,AQP2 |
| R2888 |
T11107 |
T11106 |
punct |
-,type |
| R2889 |
T10960 |
T10956 |
compound |
mutant,protein |
| R2890 |
T11108 |
T11104 |
dobj |
AQP2,expressing |
| R2891 |
T11109 |
T11110 |
punct |
(,blots |
| R2892 |
T10961 |
T10956 |
punct |
", ",protein |
| R2893 |
T11110 |
T11070 |
parataxis |
blots,coimmunoprecipitated |
| R2894 |
T11111 |
T11110 |
dep |
Figure,blots |
| R2895 |
T11112 |
T11111 |
nummod |
5B,Figure |
| R2896 |
T10962 |
T10963 |
compound |
AQP2,P262L |
| R2897 |
T11113 |
T11110 |
punct |
", ",blots |
| R2898 |
T11114 |
T11110 |
amod |
upper,blots |
| R2899 |
T11115 |
T11110 |
punct |
),blots |
| R2900 |
T10963 |
T10956 |
appos |
P262L,protein |
| R2901 |
T11116 |
T11070 |
punct |
.,coimmunoprecipitated |
| R2902 |
T10964 |
T10963 |
punct |
-,P262L |
| R2903 |
T11118 |
T11119 |
compound |
Western,blot |
| R2904 |
T11119 |
T11120 |
nsubj |
blot,showed |
| R2905 |
T10965 |
T10948 |
punct |
", ",rescue |
| R2906 |
T11121 |
T11119 |
prep |
of,blot |
| R2907 |
T11122 |
T11123 |
amod |
total,membranes |
| R2908 |
T11123 |
T11121 |
pobj |
membranes,of |
| R2909 |
T10966 |
T10967 |
advmod |
when,coexpressed |
| R2910 |
T11124 |
T11125 |
mark |
that,expressed |
| R2911 |
T11125 |
T11120 |
ccomp |
expressed,showed |
| R2912 |
T11126 |
T11127 |
amod |
wild,type |
| R2913 |
T10967 |
T10948 |
advcl |
coexpressed,rescue |
| R2914 |
T11127 |
T11129 |
compound |
type,AQP2 |
| R2915 |
T11128 |
T11127 |
punct |
-,type |
| R2916 |
T11129 |
T11125 |
nsubjpass |
AQP2,expressed |
| R2917 |
T10968 |
T10969 |
det |
the,two |
| R2918 |
T11130 |
T11125 |
auxpass |
is,expressed |
| R2919 |
T11131 |
T11125 |
advmod |
equivalently,expressed |
| R2920 |
T10969 |
T10967 |
nsubjpass |
two,coexpressed |
| R2921 |
T11132 |
T11125 |
prep |
in,expressed |
| R2922 |
T11133 |
T11134 |
det |
all,cases |
| R2923 |
T11134 |
T11132 |
pobj |
cases,in |
| R2924 |
T11135 |
T11134 |
nummod |
three,cases |
| R2925 |
T10970 |
T10967 |
auxpass |
are,coexpressed |
| R2926 |
T11136 |
T11137 |
punct |
(,blots |
| R2927 |
T11137 |
T11120 |
parataxis |
blots,showed |
| R2928 |
T11138 |
T11137 |
dep |
Figure,blots |
| R2929 |
T11139 |
T11138 |
nummod |
5B,Figure |
| R2930 |
T11140 |
T11137 |
punct |
", ",blots |
| R2931 |
T11141 |
T11137 |
amod |
lower,blots |
| R2932 |
T10971 |
T10967 |
prep |
in,coexpressed |
| R2933 |
T11142 |
T11137 |
punct |
),blots |
| R2934 |
T11143 |
T11120 |
punct |
.,showed |
| R2935 |
T11145 |
T11146 |
mark |
If,interacting |
| R2936 |
T10972 |
T10973 |
compound |
MDCK,cells |
| R2937 |
T10973 |
T10971 |
pobj |
cells,in |
| R2938 |
T11146 |
T11155 |
advcl |
interacting,is |
| R2939 |
T10974 |
T10975 |
punct |
[,25 |
| R2940 |
T11147 |
T11148 |
amod |
wild,type |
| R2941 |
T11148 |
T11150 |
nmod |
type,proteins |
| R2942 |
T11149 |
T11148 |
punct |
-,type |
| R2943 |
T11150 |
T11146 |
nsubj |
proteins,interacting |
| R2944 |
T10975 |
T10943 |
parataxis |
25,shown |
| R2945 |
T11151 |
T11148 |
cc |
and,type |
| R2946 |
T11152 |
T11148 |
conj |
mutant,type |
| R2947 |
T11153 |
T11146 |
aux |
are,interacting |
| R2948 |
T10976 |
T10975 |
punct |
],25 |
| R2949 |
T11154 |
T11146 |
advmod |
indeed,interacting |
| R2950 |
T11156 |
T11146 |
prep |
in,interacting |
| R2951 |
T10977 |
T10943 |
punct |
.,shown |
| R2952 |
T11157 |
T11158 |
det |
the,cell |
| R2953 |
T11158 |
T11156 |
pobj |
cell,in |
| R2954 |
T11159 |
T11155 |
punct |
", ",is |
| R2955 |
T10979 |
T10980 |
prep |
In,rescue |
| R2956 |
T11160 |
T11161 |
det |
this,interaction |
| R2957 |
T11161 |
T11155 |
nsubj |
interaction,is |
| R2958 |
T11162 |
T11155 |
acomp |
sufficient,is |
| R2959 |
T11163 |
T11164 |
aux |
to,rescue |
| R2960 |
T10981 |
T10982 |
det |
the,ducts |
| R2961 |
T11164 |
T11162 |
xcomp |
rescue,sufficient |
| R2962 |
T11165 |
T11166 |
det |
the,localization |
| R2963 |
T11166 |
T11164 |
dobj |
localization,rescue |
| R2964 |
T10982 |
T10979 |
pobj |
ducts,In |
| R2965 |
T11167 |
T11166 |
prep |
of,localization |
| R2966 |
T11168 |
T11169 |
compound |
mutant,protein |
| R2967 |
T11169 |
T11167 |
pobj |
protein,of |
| R2968 |
T11170 |
T11155 |
punct |
?,is |
| R2969 |
T10983 |
T10982 |
amod |
collecting,ducts |
| R2970 |
T11172 |
T11173 |
aux |
To,answer |
| R2971 |
T11173 |
T11174 |
advcl |
answer,used |
| R2972 |
T10984 |
T10982 |
prep |
from,ducts |
| R2973 |
T11175 |
T11176 |
det |
this,question |
| R2974 |
T11176 |
T11173 |
dobj |
question,answer |
| R2975 |
T10985 |
T10986 |
nmod |
Aqp2F204V,mice |
| R2976 |
T11177 |
T11174 |
punct |
", ",used |
| R2977 |
T11178 |
T11174 |
nsubj |
we,used |
| R2978 |
T10986 |
T10984 |
pobj |
mice,from |
| R2979 |
T11179 |
T11180 |
compound |
MDCK,cells |
| R2980 |
T11180 |
T11174 |
dobj |
cells,used |
| R2981 |
T11181 |
T11182 |
advmod |
stably,transfected |
| R2982 |
T11182 |
T11180 |
acl |
transfected,cells |
| R2983 |
T11183 |
T11182 |
prep |
with,transfected |
| R2984 |
T11184 |
T11185 |
amod |
wild,type |
| R2985 |
T10987 |
T10985 |
punct |
/,Aqp2F204V |
| R2986 |
T11185 |
T11187 |
compound |
type,AQP2 |
| R2987 |
T11186 |
T11185 |
punct |
-,type |
| R2988 |
T11187 |
T11183 |
pobj |
AQP2,with |
| R2989 |
T10988 |
T10985 |
punct |
+,Aqp2F204V |
| R2990 |
T11188 |
T11187 |
acl |
expressing,AQP2 |
| R2991 |
T11189 |
T11188 |
dobj |
vector,expressing |
| R2992 |
T11190 |
T11188 |
cc |
or,expressing |
| R2993 |
T10989 |
T10980 |
punct |
", ",rescue |
| R2994 |
T11191 |
T11188 |
conj |
with,expressing |
| R2995 |
T11192 |
T11193 |
amod |
empty,vector |
| R2996 |
T11193 |
T11191 |
pobj |
vector,with |
| R2997 |
T10990 |
T10991 |
det |
the,type |
| R2998 |
T11194 |
T11174 |
punct |
.,used |
| R2999 |
T11196 |
T11197 |
prep |
On,transfected |
| R3000 |
T11203 |
T11197 |
advmod |
transiently,transfected |
| R3001 |
T11198 |
T11196 |
pobj |
top,On |
| R3002 |
T11199 |
T11198 |
prep |
of,top |
| R3003 |
T11200 |
T11199 |
pobj |
these,of |
| R3004 |
T11201 |
T11197 |
punct |
", ",transfected |
| R3005 |
T11204 |
T11205 |
nmod |
AQP2,GFP |
| R3006 |
T11202 |
T11197 |
nsubj |
we,transfected |
| R3007 |
T11205 |
T11207 |
nmod |
GFP,constructs |
| R3008 |
T11206 |
T11205 |
punct |
-,GFP |
| R3009 |
T11207 |
T11197 |
dobj |
constructs,transfected |
| R3010 |
T11208 |
T11205 |
cc |
or,GFP |
| R3011 |
T11308 |
T11306 |
compound |
F204V,GFP |
| R3012 |
T11309 |
T11306 |
punct |
-,GFP |
| R3013 |
T11209 |
T11210 |
compound |
AQP2,GFP |
| R3014 |
T11310 |
T11295 |
prep |
to,localized |
| R3015 |
T11311 |
T11312 |
det |
the,surface |
| R3016 |
T11312 |
T11310 |
pobj |
surface,to |
| R3017 |
T11210 |
T11205 |
conj |
GFP,GFP |
| R3018 |
T11313 |
T11312 |
amod |
apical,surface |
| R3019 |
T11314 |
T11295 |
prep |
to,localized |
| R3020 |
T11211 |
T11210 |
punct |
-,GFP |
| R3021 |
T11315 |
T11316 |
amod |
varying,degrees |
| R3022 |
T11316 |
T11314 |
pobj |
degrees,to |
| R3023 |
T11317 |
T11318 |
punct |
(,images |
| R3024 |
T11212 |
T11210 |
compound |
F204V,GFP |
| R3025 |
T11318 |
T11295 |
parataxis |
images,localized |
| R3026 |
T11319 |
T11318 |
dep |
Figure,images |
| R3027 |
T11320 |
T11319 |
nummod |
5C,Figure |
| R3028 |
T11213 |
T11210 |
punct |
-,GFP |
| R3029 |
T11321 |
T11318 |
punct |
", ",images |
| R3030 |
T11322 |
T11318 |
amod |
lower,images |
| R3031 |
T11214 |
T11207 |
compound |
expression,constructs |
| R3032 |
T11323 |
T11318 |
amod |
right,images |
| R3033 |
T11324 |
T11325 |
punct |
[,i |
| R3034 |
T11325 |
T11318 |
parataxis |
i,images |
| R3035 |
T11215 |
T11197 |
punct |
.,transfected |
| R3036 |
T11326 |
T11327 |
punct |
–,iii |
| R3037 |
T11327 |
T11325 |
prep |
iii,i |
| R3038 |
T11217 |
T11218 |
compound |
AQP2,GFP |
| R3039 |
T11328 |
T11325 |
punct |
],i |
| R3040 |
T11329 |
T11318 |
punct |
),images |
| R3041 |
T11330 |
T11295 |
punct |
.,localized |
| R3042 |
T11218 |
T11220 |
nsubj |
GFP,localized |
| R3043 |
T11332 |
T11333 |
det |
The,images |
| R3044 |
T11333 |
T11336 |
nsubj |
images,shows |
| R3045 |
T11219 |
T11218 |
punct |
-,GFP |
| R3046 |
T11334 |
T11335 |
amod |
lower,right |
| R3047 |
T11335 |
T11333 |
amod |
right,images |
| R3048 |
T11221 |
T11220 |
prep |
to,localized |
| R3049 |
T11337 |
T11333 |
prep |
of,images |
| R3050 |
T11338 |
T11337 |
pobj |
Figure,of |
| R3051 |
T11339 |
T11338 |
nummod |
5C,Figure |
| R3052 |
T11222 |
T11223 |
det |
the,surface |
| R3053 |
T11340 |
T11341 |
nummod |
three,cells |
| R3054 |
T11341 |
T11336 |
dobj |
cells,shows |
| R3055 |
T11342 |
T11341 |
prep |
from,cells |
| R3056 |
T11223 |
T11221 |
pobj |
surface,to |
| R3057 |
T11343 |
T11344 |
det |
a,transfection |
| R3058 |
T11344 |
T11342 |
pobj |
transfection,from |
| R3059 |
T11345 |
T11344 |
amod |
single,transfection |
| R3060 |
T11224 |
T11223 |
amod |
apical,surface |
| R3061 |
T11346 |
T11336 |
punct |
.,shows |
| R3062 |
T11348 |
T11349 |
det |
The,first |
| R3063 |
T11225 |
T11220 |
prep |
upon,localized |
| R3064 |
T11349 |
T11350 |
nsubj |
first,is |
| R3065 |
T11226 |
T11227 |
compound |
forskolin,stimulation |
| R3066 |
T11351 |
T11352 |
det |
a,cell |
| R3067 |
T11352 |
T11350 |
attr |
cell,is |
| R3068 |
T11353 |
T11352 |
amod |
nontransfected,cell |
| R3069 |
T11227 |
T11225 |
pobj |
stimulation,upon |
| R3070 |
T11354 |
T11355 |
dep |
that,shows |
| R3071 |
T11355 |
T11352 |
relcl |
shows,cell |
| R3072 |
T11356 |
T11357 |
det |
the,localization |
| R3073 |
T11228 |
T11229 |
mark |
whether,transfected |
| R3074 |
T11357 |
T11355 |
dobj |
localization,shows |
| R3075 |
T11358 |
T11357 |
prep |
of,localization |
| R3076 |
T11359 |
T11360 |
det |
the,AQP2 |
| R3077 |
T11229 |
T11220 |
advcl |
transfected,localized |
| R3078 |
T11360 |
T11358 |
pobj |
AQP2,of |
| R3079 |
T11230 |
T11229 |
nsubjpass |
it,transfected |
| R3080 |
T11361 |
T11362 |
advmod |
stably,expressing |
| R3081 |
T11231 |
T11229 |
auxpass |
was,transfected |
| R3082 |
T11362 |
T11360 |
amod |
expressing,AQP2 |
| R3083 |
T11363 |
T11364 |
amod |
wild,type |
| R3084 |
T11232 |
T11229 |
advmod |
transiently,transfected |
| R3085 |
T11364 |
T11360 |
compound |
type,AQP2 |
| R3086 |
T11365 |
T11364 |
punct |
-,type |
| R3087 |
T11366 |
T11360 |
punct |
", ",AQP2 |
| R3088 |
T11367 |
T11368 |
dep |
which,is |
| R3089 |
T11368 |
T11360 |
relcl |
is,AQP2 |
| R3090 |
T11233 |
T11229 |
prep |
into,transfected |
| R3091 |
T11369 |
T11368 |
acomp |
apical,is |
| R3092 |
T11370 |
T11368 |
prep |
upon,is |
| R3093 |
T11371 |
T11372 |
compound |
forskolin,stimulation |
| R3094 |
T11234 |
T11235 |
npadvmod |
vector,only |
| R3095 |
T11372 |
T11370 |
pobj |
stimulation,upon |
| R3096 |
T11373 |
T11350 |
punct |
.,is |
| R3097 |
T11235 |
T11237 |
amod |
only,cells |
| R3098 |
T11375 |
T11376 |
det |
The,two |
| R3099 |
T11376 |
T11378 |
nsubj |
two,show |
| R3100 |
T11377 |
T11376 |
amod |
next,two |
| R3101 |
T11236 |
T11235 |
punct |
-,only |
| R3102 |
T11379 |
T11378 |
dobj |
expression,show |
| R3103 |
T11380 |
T11379 |
prep |
of,expression |
| R3104 |
T11237 |
T11233 |
pobj |
cells,into |
| R3105 |
T11381 |
T11382 |
preconj |
both,AQP2 |
| R3106 |
T11382 |
T11380 |
pobj |
AQP2,of |
| R3107 |
T11238 |
T11239 |
punct |
(,images |
| R3108 |
T11383 |
T11382 |
det |
the,AQP2 |
| R3109 |
T11384 |
T11382 |
amod |
stable,AQP2 |
| R3110 |
T11385 |
T11386 |
amod |
wild,type |
| R3111 |
T11239 |
T11237 |
parataxis |
images,cells |
| R3112 |
T11386 |
T11382 |
compound |
type,AQP2 |
| R3113 |
T11387 |
T11386 |
punct |
-,type |
| R3114 |
T11388 |
T11382 |
cc |
and,AQP2 |
| R3115 |
T11240 |
T11239 |
dep |
Figure,images |
| R3116 |
T11389 |
T11390 |
det |
the,GFP |
| R3117 |
T11390 |
T11382 |
conj |
GFP,AQP2 |
| R3118 |
T11391 |
T11390 |
amod |
transient,GFP |
| R3119 |
T11241 |
T11240 |
nummod |
5C,Figure |
| R3120 |
T11392 |
T11390 |
compound |
AQP2,GFP |
| R3121 |
T11393 |
T11390 |
punct |
-,GFP |
| R3122 |
T11394 |
T11390 |
compound |
F204V,GFP |
| R3123 |
T11242 |
T11239 |
punct |
", ",images |
| R3124 |
T11395 |
T11390 |
punct |
-,GFP |
| R3125 |
T11396 |
T11378 |
punct |
.,show |
| R3126 |
T11243 |
T11239 |
amod |
upper,images |
| R3127 |
T11398 |
T11399 |
prep |
In,was |
| R3128 |
T11399 |
T11411 |
ccomp |
was,were |
| R3129 |
T11244 |
T11239 |
amod |
left,images |
| R3130 |
T11400 |
T11398 |
pobj |
cell,In |
| R3131 |
T11401 |
T11400 |
punct |
(,cell |
| R3132 |
T11402 |
T11400 |
nummod |
ii,cell |
| R3133 |
T11403 |
T11400 |
punct |
),cell |
| R3134 |
T11245 |
T11239 |
punct |
),images |
| R3135 |
T11404 |
T11399 |
punct |
", ",was |
| R3136 |
T11405 |
T11399 |
nsubj |
localization,was |
| R3137 |
T11406 |
T11405 |
prep |
of,localization |
| R3138 |
T11407 |
T11408 |
amod |
wild,type |
| R3139 |
T11408 |
T11410 |
compound |
type,AQP2 |
| R3140 |
T11409 |
T11408 |
punct |
-,type |
| R3141 |
T11246 |
T11233 |
cc |
or,into |
| R3142 |
T11410 |
T11406 |
pobj |
AQP2,of |
| R3143 |
T11412 |
T11399 |
acomp |
indistinguishable,was |
| R3144 |
T11247 |
T11233 |
conj |
into,into |
| R3145 |
T11248 |
T11249 |
amod |
wild,type |
| R3146 |
T11249 |
T11251 |
compound |
type,cells |
| R3147 |
T11413 |
T11412 |
prep |
from,indistinguishable |
| R3148 |
T11414 |
T11415 |
compound |
AQP2,GFP |
| R3149 |
T11250 |
T11249 |
punct |
-,type |
| R3150 |
T11251 |
T11247 |
pobj |
cells,into |
| R3151 |
T11415 |
T11413 |
pobj |
GFP,from |
| R3152 |
T11416 |
T11415 |
punct |
-,GFP |
| R3153 |
T11252 |
T11251 |
compound |
AQP2,cells |
| R3154 |
T11417 |
T11415 |
compound |
F204V,GFP |
| R3155 |
T11418 |
T11415 |
punct |
-,GFP |
| R3156 |
T11419 |
T11411 |
punct |
;,were |
| R3157 |
T11253 |
T11254 |
punct |
(,Figure |
| R3158 |
T11420 |
T11411 |
nsubj |
both,were |
| R3159 |
T11421 |
T11411 |
acomp |
apical,were |
| R3160 |
T11254 |
T11251 |
parataxis |
Figure,cells |
| R3161 |
T11422 |
T11411 |
prep |
upon,were |
| R3162 |
T11423 |
T11424 |
compound |
forskolin,stimulation |
| R3163 |
T11255 |
T11254 |
nummod |
5C,Figure |
| R3164 |
T11424 |
T11422 |
pobj |
stimulation,upon |
| R3165 |
T11425 |
T11411 |
punct |
.,were |
| R3166 |
T11256 |
T11254 |
punct |
", ",Figure |
| R3167 |
T11427 |
T11428 |
mark |
Although,was |
| R3168 |
T11428 |
T11431 |
advcl |
was,localized |
| R3169 |
T11257 |
T11258 |
amod |
upper,right |
| R3170 |
T11429 |
T11430 |
det |
the,effect |
| R3171 |
T11430 |
T11428 |
nsubj |
effect,was |
| R3172 |
T11258 |
T11254 |
amod |
right,Figure |
| R3173 |
T11432 |
T11428 |
acomp |
subtle,was |
| R3174 |
T11433 |
T11428 |
prep |
in,was |
| R3175 |
T11259 |
T11254 |
punct |
),Figure |
| R3176 |
T11434 |
T11433 |
pobj |
cell,in |
| R3177 |
T11435 |
T11434 |
punct |
(,cell |
| R3178 |
T11436 |
T11434 |
nummod |
iii,cell |
| R3179 |
T11260 |
T11220 |
punct |
.,localized |
| R3180 |
T11437 |
T11434 |
punct |
),cell |
| R3181 |
T11438 |
T11431 |
punct |
", ",localized |
| R3182 |
T11439 |
T11440 |
compound |
AQP2,GFP |
| R3183 |
T11262 |
T11263 |
compound |
AQP2,GFP |
| R3184 |
T11440 |
T11431 |
nsubjpass |
GFP,localized |
| R3185 |
T11441 |
T11440 |
punct |
-,GFP |
| R3186 |
T11442 |
T11440 |
compound |
F204V,GFP |
| R3187 |
T11443 |
T11440 |
punct |
-,GFP |
| R3188 |
T11444 |
T11431 |
auxpass |
was,localized |
| R3189 |
T11445 |
T11431 |
advmod |
partly,localized |
| R3190 |
T11446 |
T11431 |
prep |
to,localized |
| R3191 |
T11263 |
T11267 |
nsubj |
GFP,showed |
| R3192 |
T11447 |
T11448 |
det |
the,surface |
| R3193 |
T11448 |
T11446 |
pobj |
surface,to |
| R3194 |
T11449 |
T11448 |
amod |
apical,surface |
| R3195 |
T11264 |
T11263 |
punct |
-,GFP |
| R3196 |
T11450 |
T11431 |
punct |
.,localized |
| R3197 |
T11452 |
T11453 |
advmod |
Generally,was |
| R3198 |
T11265 |
T11263 |
compound |
F204V,GFP |
| R3199 |
T11454 |
T11453 |
punct |
", ",was |
| R3200 |
T11266 |
T11263 |
punct |
-,GFP |
| R3201 |
T11455 |
T11456 |
det |
the,localization |
| R3202 |
T11456 |
T11453 |
nsubj |
localization,was |
| R3203 |
T11457 |
T11456 |
prep |
of,localization |
| R3204 |
T11268 |
T11267 |
punct |
", ",showed |
| R3205 |
T11458 |
T11459 |
compound |
AQP2,GFP |
| R3206 |
T11459 |
T11457 |
pobj |
GFP,of |
| R3207 |
T11460 |
T11459 |
punct |
-,GFP |
| R3208 |
T11461 |
T11459 |
compound |
F204V,GFP |
| R3209 |
T11269 |
T11270 |
advmod |
when,expressed |
| R3210 |
T11462 |
T11459 |
punct |
-,GFP |
| R3211 |
T11463 |
T11453 |
advmod |
clearly,was |
| R3212 |
T11464 |
T11465 |
advmod |
more,apical |
| R3213 |
T11270 |
T11267 |
advcl |
expressed,showed |
| R3214 |
T11465 |
T11453 |
acomp |
apical,was |
| R3215 |
T11466 |
T11467 |
advmod |
when,expressed |
| R3216 |
T11467 |
T11453 |
advcl |
expressed,was |
| R3217 |
T11271 |
T11270 |
prep |
by,expressed |
| R3218 |
T11468 |
T11469 |
amod |
wild,type |
| R3219 |
T11469 |
T11471 |
compound |
type,AQP2 |
| R3220 |
T11470 |
T11469 |
punct |
-,type |
| R3221 |
T11272 |
T11273 |
amod |
transient,transfection |
| R3222 |
T11471 |
T11467 |
nsubjpass |
AQP2,expressed |
| R3223 |
T11472 |
T11467 |
auxpass |
was,expressed |
| R3224 |
T11473 |
T11467 |
advmod |
also,expressed |
| R3225 |
T11273 |
T11271 |
pobj |
transfection,by |
| R3226 |
T11474 |
T11453 |
punct |
.,was |
| R3227 |
T11274 |
T11270 |
prep |
into,expressed |
| R3228 |
T11476 |
T11477 |
aux |
To,confirm |
| R3229 |
T11477 |
T11478 |
advcl |
confirm,transfected |
| R3230 |
T11275 |
T11276 |
npadvmod |
vector,only |
| R3231 |
T11479 |
T11480 |
det |
these,results |
| R3232 |
T11480 |
T11477 |
dobj |
results,confirm |
| R3233 |
T11276 |
T11277 |
amod |
only,cells |
| R3234 |
T11481 |
T11477 |
advmod |
biochemically,confirm |
| R3235 |
T11482 |
T11478 |
punct |
", ",transfected |
| R3236 |
T11483 |
T11478 |
nsubj |
we,transfected |
| R3237 |
T11277 |
T11274 |
pobj |
cells,into |
| R3238 |
T11484 |
T11485 |
det |
the,lines |
| R3239 |
T11485 |
T11478 |
dobj |
lines,transfected |
| R3240 |
T11486 |
T11485 |
amod |
same,lines |
| R3241 |
T11487 |
T11485 |
compound |
cell,lines |
| R3242 |
T11488 |
T11485 |
punct |
(,lines |
| R3243 |
T11278 |
T11267 |
punct |
", ",showed |
| R3244 |
T11489 |
T11490 |
amod |
wild,type |
| R3245 |
T11490 |
T11492 |
compound |
type,AQP2 |
| R3246 |
T11491 |
T11490 |
punct |
-,type |
| R3247 |
T11279 |
T11280 |
det |
a,pattern |
| R3248 |
T11492 |
T11485 |
appos |
AQP2,lines |
| R3249 |
T11493 |
T11492 |
cc |
or,AQP2 |
| R3250 |
T11494 |
T11492 |
conj |
vector,AQP2 |
| R3251 |
T11280 |
T11267 |
dobj |
pattern,showed |
| R3252 |
T11495 |
T11485 |
punct |
),lines |
| R3253 |
T11496 |
T11478 |
prep |
with,transfected |
| R3254 |
T11497 |
T11498 |
compound |
F204V,GFP |
| R3255 |
T11281 |
T11280 |
amod |
diffuse,pattern |
| R3256 |
T11498 |
T11496 |
pobj |
GFP,with |
| R3257 |
T11499 |
T11498 |
punct |
-,GFP |
| R3258 |
T11500 |
T11478 |
punct |
", ",transfected |
| R3259 |
T11282 |
T11280 |
amod |
cytoplasmic,pattern |
| R3260 |
T11501 |
T11478 |
conj |
biotinylated,transfected |
| R3261 |
T11283 |
T11280 |
compound |
distribution,pattern |
| R3262 |
T11502 |
T11503 |
compound |
surface,proteins |
| R3263 |
T11284 |
T11285 |
punct |
(,left |
| R3264 |
T11503 |
T11501 |
dobj |
proteins,biotinylated |
| R3265 |
T11504 |
T11501 |
prep |
after,biotinylated |
| R3266 |
T11505 |
T11506 |
compound |
forskolin,stimulation |
| R3267 |
T11285 |
T11267 |
parataxis |
left,showed |
| R3268 |
T11506 |
T11504 |
pobj |
stimulation,after |
| R3269 |
T11507 |
T11501 |
punct |
", ",biotinylated |
| R3270 |
T11286 |
T11285 |
dep |
Figure,left |
| R3271 |
T11508 |
T11501 |
cc |
and,biotinylated |
| R3272 |
T11509 |
T11501 |
conj |
precipitated,biotinylated |
| R3273 |
T11510 |
T11511 |
det |
the,proteins |
| R3274 |
T11287 |
T11286 |
nummod |
5C,Figure |
| R3275 |
T11511 |
T11509 |
dobj |
proteins,precipitated |
| R3276 |
T11512 |
T11511 |
amod |
biotinylated,proteins |
| R3277 |
T11288 |
T11285 |
punct |
", ",left |
| R3278 |
T11513 |
T11514 |
punct |
(,Figure |
| R3279 |
T11514 |
T11509 |
parataxis |
Figure,precipitated |
| R3280 |
T11515 |
T11514 |
nummod |
5D,Figure |
| R3281 |
T11289 |
T11285 |
amod |
lower,left |
| R3282 |
T11516 |
T11514 |
punct |
),Figure |
| R3283 |
T11517 |
T11478 |
punct |
.,transfected |
| R3284 |
T11290 |
T11285 |
punct |
),left |
| R3285 |
T11291 |
T11267 |
punct |
.,showed |
| R3286 |
T11293 |
T11294 |
advmod |
When,expressed |
| R3287 |
T11519 |
T11520 |
compound |
AQP2,GFP |
| R3288 |
T11520 |
T11524 |
nsubjpass |
GFP,expressed |
| R3289 |
T11294 |
T11295 |
advcl |
expressed,localized |
| R3290 |
T11521 |
T11520 |
punct |
-,GFP |
| R3291 |
T11522 |
T11520 |
compound |
F204V,GFP |
| R3292 |
T11523 |
T11520 |
punct |
-,GFP |
| R3293 |
T11525 |
T11524 |
auxpass |
is,expressed |
| R3294 |
T11296 |
T11294 |
prep |
in,expressed |
| R3295 |
T11526 |
T11527 |
advmod |
approximately,equally |
| R3296 |
T11527 |
T11524 |
advmod |
equally,expressed |
| R3297 |
T11528 |
T11524 |
prep |
in,expressed |
| R3298 |
T11529 |
T11530 |
det |
both,lines |
| R3299 |
T11530 |
T11528 |
pobj |
lines,in |
| R3300 |
T11297 |
T11298 |
amod |
wild,type |
| R3301 |
T11531 |
T11530 |
compound |
cell,lines |
| R3302 |
T11532 |
T11533 |
punct |
(,cells |
| R3303 |
T11533 |
T11524 |
parataxis |
cells,expressed |
| R3304 |
T11298 |
T11300 |
compound |
type,cells |
| R3305 |
T11534 |
T11533 |
dep |
Figure,cells |
| R3306 |
T11535 |
T11534 |
nummod |
5D,Figure |
| R3307 |
T11536 |
T11533 |
punct |
", ",cells |
| R3308 |
T11299 |
T11298 |
punct |
-,type |
| R3309 |
T11537 |
T11533 |
amod |
total,cells |
| R3310 |
T11538 |
T11533 |
punct |
),cells |
| R3311 |
T11300 |
T11296 |
pobj |
cells,in |
| R3312 |
T11539 |
T11524 |
punct |
", ",expressed |
| R3313 |
T11540 |
T11524 |
cc |
but,expressed |
| R3314 |
T11541 |
T11542 |
auxpass |
is,biotinylated |
| R3315 |
T11301 |
T11300 |
compound |
AQP2,cells |
| R3316 |
T11542 |
T11524 |
conj |
biotinylated,expressed |
| R3317 |
T11543 |
T11544 |
advmod |
only,expressed |
| R3318 |
T11544 |
T11542 |
ccomp |
expressed,biotinylated |
| R3319 |
T11302 |
T11295 |
punct |
", ",localized |
| R3320 |
T11545 |
T11544 |
advmod |
when,expressed |
| R3321 |
T11546 |
T11547 |
amod |
wild,type |
| R3322 |
T11547 |
T11549 |
compound |
type,AQP2 |
| R3323 |
T11303 |
T11295 |
advmod |
however,localized |
| R3324 |
T11548 |
T11547 |
punct |
-,type |
| R3325 |
T11549 |
T11544 |
nsubjpass |
AQP2,expressed |
| R3326 |
T11550 |
T11544 |
auxpass |
is,expressed |
| R3327 |
T11304 |
T11295 |
punct |
", ",localized |
| R3328 |
T11551 |
T11544 |
advmod |
also,expressed |
| R3329 |
T11552 |
T11553 |
punct |
(,Figure |
| R3330 |
T11553 |
T11542 |
parataxis |
Figure,biotinylated |
| R3331 |
T11305 |
T11306 |
compound |
AQP2,GFP |
| R3332 |
T11554 |
T11553 |
nummod |
5D,Figure |
| R3333 |
T11555 |
T11553 |
punct |
", ",Figure |
| R3334 |
T11556 |
T11557 |
npadvmod |
surface,biotinylated |
| R3335 |
T11306 |
T11295 |
nsubj |
GFP,localized |
| R3336 |
T11557 |
T11553 |
amod |
biotinylated,Figure |
| R3337 |
T11558 |
T11553 |
punct |
),Figure |
| R3338 |
T11559 |
T11524 |
punct |
.,expressed |
| R3339 |
T11307 |
T11306 |
punct |
-,GFP |
| R3340 |
T11561 |
T11562 |
mark |
Since,are |
| R3341 |
T11562 |
T11567 |
advcl |
are,indicate |
| R3342 |
T11563 |
T11564 |
advmod |
only,proteins |
| R3343 |
T11564 |
T11562 |
nsubj |
proteins,are |
| R3344 |
T11565 |
T11566 |
compound |
cell,surface |
| R3345 |
T11566 |
T11564 |
compound |
surface,proteins |
| R3346 |
T11568 |
T11562 |
acomp |
accessible,are |
| R3347 |
T11569 |
T11568 |
prep |
to,accessible |
| R3348 |
T11570 |
T11569 |
pobj |
biotin,to |
| R3349 |
T11571 |
T11567 |
punct |
", ",indicate |
| R3350 |
T11572 |
T11573 |
det |
these,results |
| R3351 |
T11573 |
T11567 |
nsubj |
results,indicate |
| R3352 |
T11574 |
T11575 |
mark |
that,transported |
| R3353 |
T11575 |
T11567 |
ccomp |
transported,indicate |
| R3354 |
T11576 |
T11577 |
compound |
AQP2,F204V |
| R3355 |
T11577 |
T11575 |
nsubjpass |
F204V,transported |
| R3356 |
T11578 |
T11577 |
punct |
-,F204V |
| R3357 |
T11579 |
T11575 |
auxpass |
is,transported |
| R3358 |
T11580 |
T11575 |
prep |
to,transported |
| R3359 |
T11581 |
T11582 |
det |
the,surface |
| R3360 |
T11582 |
T11580 |
pobj |
surface,to |
| R3361 |
T11583 |
T11582 |
compound |
cell,surface |
| R3362 |
T11584 |
T11585 |
advmod |
when,is |
| R3363 |
T11585 |
T11575 |
advcl |
is,transported |
| R3364 |
T11586 |
T11587 |
amod |
wild,type |
| R3365 |
T11587 |
T11589 |
compound |
type,AQP2 |
| R3366 |
T11588 |
T11587 |
punct |
-,type |
| R3367 |
T11589 |
T11585 |
nsubj |
AQP2,is |
| R3368 |
T11590 |
T11585 |
acomp |
present,is |
| R3369 |
T11591 |
T11585 |
punct |
", ",is |
| R3370 |
T11592 |
T11585 |
cc |
but,is |
| R3371 |
T11593 |
T11592 |
neg |
not,but |
| R3372 |
T11594 |
T11585 |
conj |
on,is |
| R3373 |
T11595 |
T11596 |
poss |
its,own |
| R3374 |
T11596 |
T11594 |
pobj |
own,on |
| R3375 |
T11597 |
T11567 |
punct |
.,indicate |
