PMC:1189073 / 6697-25762 JSON TXT

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craft-ca-core-ex-dev

Below, discontinuous spans are shown in the bag model. You can change it to the chain model.

Id	Subject	Object	Predicate	Lexical cue
T8418	561-574	SO_EXT:coding_sequence	denotes	coding region
T8419	21-28	SO_EXT:0000704	denotes	genetic
T8420	46-62	CHEBI:23995	denotes	ethylnitrosourea
T8421	64-67	CHEBI:23995	denotes	ENU
T8422	79-88	SO_EXT:sequence_alteration_entity_or_process	denotes	mutations
T8423	102-108	NCBITaxon:33208	denotes	animal
T8424	163-167	UBERON_EXT:body	denotes	body
T8425	168-178	GO:0008152	denotes	metabolism
T8426	209-213	NCBITaxon:10088	denotes	mice
T8427	219-227	GO:0060073	denotes	urinated
T8428	232-237	GO:0042756	denotes	drank
T8429	251-256	UBERON:0001977	denotes	Serum
T8430	261-266	UBERON:0001088	denotes	urine
T8431	288-294	UBERON:0001969	denotes	plasma
T8432	295-302	CHEBI:17234	denotes	glucose
T8433	339-346	CHEBI:17234	denotes	glucose
T8434	354-359	UBERON:0001088	denotes	urine
T8435	452-456	NCBITaxon:10088	denotes	mice
T8436	477-482	SO_EXT:0000704	denotes	genic
T8437	484-493	GO:0030849	denotes	autosomal
T8438	519-523	PR_EXT:000004182	denotes	Aqp2
T8439	536-540	SO_EXT:0000704	denotes	gene
T8440	556-560	PR_EXT:000004182	denotes	Aqp2
T8441	587-591	NCBITaxon:10088	denotes	mice
T8442	605-612	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	thymine
T8444	625-631,605-623,633-645	SO_EXT:1000022	denotes	T to G thymine to guanine transversion
T8445	616-623	CHEBI_EXT:guanine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	guanine
T8446	625-626	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	T
T8448	630-631	CHEBI_EXT:guanine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	G
T8449	691-697	CHEBI_SO_EXT:valine	denotes	valine
T8450	702-715	CHEBI_SO_EXT:phenylalanine	denotes	phenylalanine
T8451	716-728	SO_EXT:sequence_substitution_entity_or_process	denotes	substitution
T8452	732-742	CHEBI_SO_EXT:amino_acid	denotes	amino acid
T8453	754-761	CHEBI_PR_EXT:protein	denotes	protein
T8454	1795-1799	PR_EXT:000004182	denotes	AQP2
T8455	1814-1822	GO:0016020	denotes	membrane
T8456	1823-1828	CHEBI:15377	denotes	water
T8457	1823-1836	GO_EXT:0015250	denotes	water channel
T8458	1861-1874	GO:0005576	denotes	extracellular
T8459	1866-1874	CL_GO_EXT:cell	denotes	cellular
T8460	1893-1901	GO:0016020	denotes	membrane
T8461	1911-1917	SO_EXT:0000417	denotes	domain
T8462	1948-1959	CHEBI_SO_EXT:amino_acid	denotes	amino acids
T8463	1980-1988	GO:0016020	denotes	membrane
T8464	1998-2005	SO_EXT:0000417	denotes	domains
T8465	2010-2019	SO_EXT:biological_conservation_process_or_quality	denotes	conserved
T8466	2026-2036	NCBITaxon:7742	denotes	vertebrate
T8467	2037-2044	NCBITaxon:species	denotes	species
T8468	2050-2063	CHEBI_SO_EXT:phenylalanine	denotes	phenylalanine
T8469	2101-2110	SO_EXT:biological_conservation_process_or_quality	denotes	conserved
T8470	2139-2149	NCBITaxon:7742	denotes	vertebrate
T8471	2150-2154	PR_EXT:000004182	denotes	AQP2
T8472	2155-2163	CHEBI_PR_EXT:protein	denotes	proteins
T8473	2211-2215	PR_EXT:000004182	denotes	Aqp2
T8474	2227-2231	NCBITaxon:10088	denotes	mice
T8475	2260-2265	UBERON:0001088	denotes	urine
T8476	2315-2320	UBERON:0001088	denotes	urine
T8477	2348-2352	UBERON_EXT:body	denotes	body
T8478	2395-2404	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T8479	2431-2436	CHEBI:15377	denotes	water
T8480	2508-2513	CHEBI:15377	denotes	water
T8481	2514-2520	GO:0007631	denotes	intake
T8482	2530-2536	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T8483	2537-2541	NCBITaxon:10088	denotes	mice
T8484	2575-2580	CHEBI:15377	denotes	water
T8485	2581-2587	GO:0007631	denotes	intake
T8486	2634-2643	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T8487	2682-2689	UBERON:0001088	denotes	urinary
T8488	2701-2706	CHEBI:15377	denotes	water
T8489	2707-2713	GO:0007631	denotes	intake
T8490	2773-2781	SO_EXT:sequence_alteration_entity_or_process	denotes	mutation
T8491	2792-2799	NCBITaxon:33208	denotes	animals
T8492	2873-2878	UBERON:0001088	denotes	urine
T8493	2910-2919	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T8494	2949-2953	PR_EXT:000004182	denotes	Aqp2
T8495	2965-2969	NCBITaxon:10088	denotes	mice
T8496	2990-2995	UBERON:0001088	denotes	urine
T8497	3015-3020	UBERON:0001088	denotes	urine
T8498	3038-3044	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T8499	3045-3049	NCBITaxon:10088	denotes	mice
T8500	3101-3110	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T8501	3111-3115	NCBITaxon:10088	denotes	mice
T8502	3139-3144	UBERON:0001088	denotes	urine
T8503	3172-3179	GO:0065007	denotes	control
T8504	3187-3199	UBERON:0001898	denotes	hypothalamus
T8505	3211-3219	GO_EXT:reaction_or_response	denotes	response
T8506	3258-3266	GO:0046903	denotes	secretes
T8507	3267-3270	CHEBI_PR_EXT:arginine_vasopressin	denotes	AVP
T8508	3286-3289	CHEBI_PR_EXT:arginine_vasopressin	denotes	AVP
T8509	3298-3332	CHEBI:4450	denotes	1-deamino-8-D-arginine vasopressin
T8510	3334-3339	CHEBI:4450	denotes	dDAVP
T8511	3353-3365	CHEBI:4450	denotes	desmopressin
T8512	3380-3387	CHEBI_GO_EXT:agonist	denotes	agonist
T8513	3391-3396	PR_EXT:000001683	denotes	AVPR2
T8514	3419-3428	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T8515	3429-3433	NCBITaxon:10088	denotes	mice
T8516	3435-3440	CHEBI:4450	denotes	dDAVP
T8517	3473-3478	UBERON:0001088	denotes	urine
T8518	3566-3572	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T8519	3573-3577	NCBITaxon:10088	denotes	mice
T8520	3596-3601	UBERON:0001088	denotes	urine
T8521	3699-3706	NCBITaxon:33208	denotes	animals
T8522	3748-3753	UBERON:0001088	denotes	urine
T8523	3795-3803	GO_EXT:reaction_or_response	denotes	response
T8525	3826-3834	GO_EXT:reaction_or_response	denotes	response
T8526	3838-3843	CHEBI:4450	denotes	dDAVP
T8527	3884-3890	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T8528	3891-3895	PR_EXT:000004182	denotes	AQP2
T8529	3896-3903	GO_EXT:0015267	denotes	channel
T8530	3955-3965	NCBITaxon:1	denotes	individual
T8531	4001-4006	NCBITaxon:10088	denotes	mouse
T8532	4035-4042	GO:0007618	denotes	matings
T8533	4055-4062	NCBITaxon:33208	denotes	animals
T8534	4131-4140	SO_EXT:wild_type_entity_or_quality	denotes	wild type
T8535	4160-4166	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T8536	4261-4269	SO_EXT:sequence_alteration_entity_or_process	denotes	mutation
T8537	4296-4301	UBERON:0001088	denotes	urine
T8538	4317-4322	CHEBI:15377	denotes	water
T8539	4323-4329	GO:0007631	denotes	intake
T8540	4363-4369	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T8541	4370-4374	NCBITaxon:10088	denotes	mice
T8542	4391-4398	UBERON:0002113	denotes	kidneys
T8543	4433-4440	NCBITaxon:33208	denotes	animals
T8544	4490-4496	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T8545	4497-4501	NCBITaxon:10088	denotes	mice
T8546	4524-4532	UBERON:0000104	denotes	lifespan
T8547	4542-4548	NCBITaxon:33208	denotes	animal
T8548	4595-4602	NCBITaxon:33208	denotes	animals
T8549	5462-5466	PR_EXT:000004182	denotes	Aqp2
T8550	5478-5482	NCBITaxon:10088	denotes	mice
T8551	5635-5642	UBERON:0002113	denotes	kidneys
T8552	5650-5654	PR_EXT:000004182	denotes	Aqp2
T8553	5666-5670	NCBITaxon:10088	denotes	mice
T8554	5730-5734	NCBITaxon:10088	denotes	mice
T8555	5819-5823	PR_EXT:000004177	denotes	Aqp1
T8556	5824-5828	PR_EXT:000004183	denotes	Aqp3
T8557	5839-5843	NCBITaxon:10088	denotes	mice
T8558	5885-5889	PR_EXT:000004182	denotes	Aqp2
T8559	5901-5905	NCBITaxon:10088	denotes	mice
T8560	5951-5955	PR_EXT:000004182	denotes	Aqp2
T8561	5967-5971	NCBITaxon:10088	denotes	mice
T8562	6010-6022	UBERON:0001224	denotes	renal pelvis
T8563	6052-6058	UBERON:0000056	denotes	ureter
T8564	6098-6116	UBERON:0006660	denotes	muscularis propria
T8565	6205-6215	UBERON:0000365	denotes	urothelium
T8566	6226-6249	UBERON:0005910	denotes	transitional epithelium
T8567	6301-6307	UBERON:0002113	denotes	kidney
T8568	6325-6338	UBERON:0002015	denotes	renal capsule
T8569	6342-6354	UBERON:0001224	denotes	renal pelvis
T8570	6397-6406	UBERON:0000074	denotes	glomeruli
T8572	6411-6419,6427-6434	UBERON:0004134	denotes	proximal tubules
T8573	6420-6434	UBERON:0004135	denotes	distal tubules
T8574	6544-6548	PR_EXT:000004182	denotes	AQP2
T8575	6588-6601	GO_MOP_EXT:glycosylation	denotes	glycosylation
T8576	6658-6670	GO_MOP_EXT:glycosylation	denotes	glycosylated
T8577	6671-6678	CHEBI_PR_EXT:protein	denotes	protein
T8578	6719-6731	GO_MOP_EXT:glycosylation	denotes	glycosylated
T8579	6732-6739	CHEBI_PR_EXT:protein	denotes	protein
T8580	6845-6852	CHEBI:37684	denotes	mannose
T8581	6853-6866	GO_MOP_EXT:glycosylation	denotes	glycosylation
T8582	6870-6874	PR_EXT:000004182	denotes	AQP2
T8583	6904-6912	CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process	denotes	labeling
T8584	6957-6964	UBERON:0002113	denotes	kidneys
T8585	6968-6977	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T8586	6978-6985	NCBITaxon:33208	denotes	animals
T8587	6987-6991	PR_EXT:000004182	denotes	AQP2
T8588	6997-7003	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T8589	7004-7011	NCBITaxon:33208	denotes	animals
T8590	7041-7050	CHEBI_EXT:polyatomic_entity_or_group	denotes	molecular
T8591	7087-7096	CHEBI_EXT:polyatomic_entity_or_group	denotes	molecular
T8592	7143-7152	CHEBI_EXT:polyatomic_entity_or_group	denotes	molecular
T8593	7191-7198	NCBITaxon:33208	denotes	animals
T8594	7267-7279	GO_MOP_EXT:glycosylation	denotes	glycosylated
T8595	7355-7362	MOP:0000780	denotes	cleaves
T8596	7363-7370	CHEBI:37684	denotes	mannose
T8597	7376-7388	CHEBI:16646	denotes	carbohydrate
T8598	7398-7405	CHEBI_PR_EXT:protein	denotes	protein
T8599	7440-7444	PR_EXT:000004182	denotes	AQP2
T8600	7450-7457	UBERON:0002113	denotes	kidneys
T8601	7461-7470	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T8602	7490-7496	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T8603	7497-7504	NCBITaxon:33208	denotes	animals
T8604	7544-7553	CHEBI_EXT:polyatomic_entity_or_group	denotes	molecular
T8605	7607-7619	GO_MOP_EXT:glycosylation	denotes	glycosylated
T8606	7620-7628	CHEBI_PR_EXT:protein	denotes	proteins
T8607	7644-7648	PR_EXT:000004182	denotes	Aqp2
T8608	7660-7664	NCBITaxon:10088	denotes	mice
T8609	7711-7715	PR_EXT:000004182	denotes	Aqp2
T8610	7727-7731	NCBITaxon:10088	denotes	mice
T8611	7769-7777	GO_EXT:reaction_or_response	denotes	response
T8612	7781-7786	CHEBI:4450	denotes	dDAVP
T8613	8292-8298	NCBITaxon:9606	denotes	humans
T8614	8310-8317	SO_EXT:0001023	denotes	alleles
T8615	8321-8325	PR_EXT:000004182	denotes	Aqp2
T8616	8383-8394	GO_EXT:biological_movement_or_translocation_process	denotes	translocate
T8617	8402-8408	GO:0045177	denotes	apical
T8618	8409-8413	CL_GO_EXT:cell	denotes	cell
T8619	8409-8421	GO:0009986	denotes	cell surface
T8620	8425-8433	GO_EXT:reaction_or_response	denotes	response
T8621	8437-8440	CHEBI_PR_EXT:arginine_vasopressin	denotes	AVP
T8622	8501-8507	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T8623	8508-8512	PR_EXT:000004182	denotes	Aqp2
T8624	8513-8518	SO_EXT:cDNA	denotes	cDNAs
T8625	8536-8541	NCBITaxon:9606	denotes	human
T8626	8550-8559	SO_EXT:sequence_alteration_entity_or_process	denotes	mutations
T8627	8564-8575	GO:0009294	denotes	transfected
T8628	8581-8587	UBERON:0002113	denotes	kidney
T8629	8581-8592	CL:1000497	denotes	kidney cell
T8630	8588-8592	CL_GO_EXT:cell	denotes	cell
T8631	8627-8634	SO_EXT:0001023	denotes	alleles
T8632	8682-8690	GO_PATO_EXT:biological_localization_process_or_quality	denotes	localize
T8633	8694-8697	CHEBI_PR_EXT:arginine_vasopressin	denotes	AVP
T8634	8709-8717	GO:0031982	denotes	vesicles
T8635	8751-8772	GO:0005783	denotes	endoplasmic reticulum
T8636	8774-8776	GO:0005783	denotes	ER
T8637	8783-8788	NCBITaxon:10088	denotes	mouse
T8638	8868-8874	NCBITaxon:33208	denotes	animal
T8639	8929-8933	PR_EXT:000004182	denotes	AQP2
T8640	8957-8966	GO_PATO_EXT:biological_localization_process_or_quality	denotes	localized
T8641	8977-8983	GO:0016324	denotes	apical
T8643	8984-8993,9010-9015	GO:0044464	denotes	region of cells
T8644	8994-9009	UBERON:0001232	denotes	collecting duct
T8645	8994-9015	CL:1001225	denotes	collecting duct cells
T8646	9019-9026	UBERON:0002113	denotes	kidneys
T8647	9030-9039	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T8648	9040-9044	NCBITaxon:10088	denotes	mice
T8649	9068-9073	CHEBI:4450	denotes	dDAVP
T8650	9075-9079	PR_EXT:000004182	denotes	AQP2
T8651	9080-9092	GO_EXT:biological_movement_or_translocation_process	denotes	translocated
T8652	9108-9112	CL_GO_EXT:cell	denotes	cell
T8653	9108-9120	GO:0009986	denotes	cell surface
T8654	9149-9156	UBERON:0002113	denotes	kidneys
T8655	9168-9174	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T8656	9175-9182	NCBITaxon:33208	denotes	animals
T8657	9193-9197	PR_EXT:000004182	denotes	AQP2
T8658	9238-9242	CL_GO_EXT:cell	denotes	cell
T8659	9292-9296	PR_EXT:000004183	denotes	AQP3
T8660	9319-9328	GO_PATO_EXT:biological_localization_process_or_quality	denotes	localized
T8661	9336-9347	GO:1990794	denotes	basolateral
T8662	9348-9355	GO:0009986	denotes	surface
T8663	9380-9385	CHEBI:4450	denotes	dDAVP
T8664	9399-9403	PR_EXT:000004182	denotes	AQP2
T8665	9420-9431	GO_EXT:biological_movement_or_translocation_process	denotes	translocate
T8666	9439-9443	CL_GO_EXT:cell	denotes	cell
T8667	9439-9451	GO:0009986	denotes	cell surface
T8668	9533-9540	UBERON:0002113	denotes	kidneys
T8669	9564-9568	NCBITaxon:10088	denotes	mice
T8670	9585-9594	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T8671	9598-9604	SO_EXT:sequence_altered_entity	denotes	mutant
T8672	9622-9627	CHEBI:4450	denotes	dDAVP
T8673	11373-11386	GO_EXT:biological_translocation_entity_or_process	denotes	translocation
T8674	11390-11394	PR_EXT:000004182	denotes	AQP2
T8675	11415-11427	GO:0009294	denotes	transfection
T8676	11436-11441	CL_GO_EXT:cell	denotes	cells
T8677	11450-11454	CL_GO_EXT:cell	denotes	cell
T8678	11461-11471	GO:0010467	denotes	expressing
T8679	11472-11477	NCBITaxon:10088	denotes	mouse
T8680	11478-11487	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T8681	11488-11492	PR_EXT:000004182	denotes	AQP2
T8682	11497-11501	PR_EXT:000004182	denotes	AQP2
T8683	11541-11548	CHEBI_PR_EXT:protein	denotes	protein
T8684	11570-11574	CL_GO_EXT:cell	denotes	cell
T8685	11598-11603	CL_GO_EXT:cell	denotes	cells
T8686	11621-11634	GO_MOP_EXT:glycosylation	denotes	glycosylation
T8687	11650-11656	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T8688	11657-11661	NCBITaxon:10088	denotes	mice
T8689	11686-11695	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T8690	11696-11703	CHEBI_PR_EXT:protein	denotes	protein
T8691	11748-11753	CL_GO_EXT:cell	denotes	Cells
T8692	11754-11764	GO:0010467	denotes	expressing
T8693	11765-11769	PR_EXT:000004182	denotes	AQP2
T8694	11832-11844	GO_MOP_EXT:glycosylation	denotes	glycosylated
T8695	11861-11872	GO:0009294	denotes	transfected
T8696	11892-11897	CL_GO_EXT:cell	denotes	cells
T8697	11899-11908	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T8698	11909-11913	PR_EXT:000004182	denotes	AQP2
T8699	11991-11997	GO:0016324	denotes	apical
T8700	12064-12073	GO:0031982	denotes	vesicular
T8701	12096-12100	PR_EXT:000004182	denotes	AQP2
T8702	12158-12165	GO:0005634	denotes	nuclear
T8703	12223-12232	CHEBI:42471	denotes	forskolin
T8704	12236-12240	CHEBI:17489	denotes	cAMP
T8705	12236-12265	GO_EXT:0004691	denotes	cAMP-dependent protein kinase
T8706	12251-12258	CHEBI_PR_EXT:protein	denotes	protein
T8707	12251-12275	GO_EXT:0030295	denotes	protein kinase activator
T8708	12277-12286	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T8709	12287-12291	PR_EXT:000004182	denotes	AQP2
T8710	12292-12304	GO_EXT:biological_movement_or_translocation_process	denotes	translocated
T8711	12312-12318	GO:0045177	denotes	apical
T8713	12319-12329,12345-12350	GO:0009986	denotes	surface of cells
T8714	12345-12350	CL_GO_EXT:cell	denotes	cells
T8715	12405-12412	GO:0005634	denotes	nuclear
T8716	12429-12433	PR_EXT:000004182	denotes	AQP2
T8717	12498-12507	CHEBI:42471	denotes	forskolin
T8718	12560-12567	GO:0005634	denotes	nuclear
T8719	12584-12588	PR_EXT:000004182	denotes	AQP2
T8720	12617-12619	GO:0005783	denotes	ER
T8721	12664-12668	PR_EXT:000004182	denotes	AQP2
T8722	12675-12684	GO_PATO_EXT:biological_localization_process_or_quality	denotes	localizes
T8723	12692-12694	GO:0005783	denotes	ER
T8724	12710-12715	CL_GO_EXT:cell	denotes	cells
T8725	12716-12727	GO:0009294	denotes	transfected
T8726	12733-12737	PR_EXT:000004182	denotes	Aqp2
T8727	12744-12748	SO_EXT:cDNA	denotes	cDNA
T8728	12754-12758	PR_EXT:000004182	denotes	AQP2
T8729	12766-12768	GO:0005783	denotes	ER
T8730	12769-12775	CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag	denotes	marker
T8731	12777-12785	PR_EXT:000005005	denotes	calnexin
T8732	12801-12813	GO_PATO_EXT:biological_localization_process_or_quality	denotes	localization
T8733	12817-12825	PR_EXT:000005005	denotes	calnexin
T8734	12831-12835	PR_EXT:000004182	denotes	AQP2
T8735	12896-12900	PR_EXT:000004182	denotes	AQP2
T8736	12907-12914	CHEBI_PR_EXT:protein	denotes	protein
T8737	12917-12926	GO_PATO_EXT:biological_localization_process_or_quality	denotes	localized
T8738	12932-12940	PR_EXT:000005005	denotes	calnexin
T8739	12993-12995	GO:0005783	denotes	ER
T8740	13010-13014	PR_EXT:000004182	denotes	AQP2
T8741	13064-13066	GO:0005783	denotes	ER
T8742	13074-13076	GO:0005783	denotes	ER
T8743	13145-13157	GO_MOP_EXT:glycosylation	denotes	glycosylated
T8744	13159-13163	PR_EXT:000004182	denotes	AQP2
T8745	13173-13179	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T8746	13180-13187	UBERON:0002113	denotes	kidneys
T8747	13205-13212	NCBITaxon:33208	denotes	Animals
T8748	13234-13238	PR_EXT:000004182	denotes	Aqp2
T8749	13244-13252	SO_EXT:sequence_alteration_entity_or_process	denotes	mutation
T8750	13280-13285	UBERON:0001088	denotes	urine
T8751	13300-13305	UBERON:0001088	denotes	urine
T8752	13385-13391	SO_EXT:0001023	denotes	allele
T8753	13393-13397	PR_EXT:000004182	denotes	AQP2
T8754	13428-13437	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T8755	13438-13445	CHEBI_PR_EXT:protein	denotes	protein
T8756	13449-13456	CL:0000023	denotes	oocytes
T8757	13475-13491	GO:0051260	denotes	homo-oligomerize
T8758	13500-13505	CL_GO_EXT:cell	denotes	cells
T8759	13523-13530	UBERON:0002113	denotes	kidneys
T8760	13549-13556	NCBITaxon:33208	denotes	animals
T8761	13606-13610	PR_EXT:000004182	denotes	AQP2
T8762	13611-13618	CHEBI_PR_EXT:protein	denotes	protein
T8763	13666-13689	UBERON:0001232	denotes	kidney collecting ducts
T8764	13695-13699	PR_EXT:000004182	denotes	Aqp2
T8765	13705-13706	SO_EXT:normal_or_wild_type_or_present	denotes	+
T8766	13707-13711	NCBITaxon:10088	denotes	mice
T8767	13753-13762	SO_EXT:wild_type_entity_or_quality	denotes	wild type
T8768	13776-13780	PR_EXT:000004182	denotes	AQP2
T8769	13781-13793	GO_EXT:biological_movement_or_translocation_process	denotes	translocated
T8770	13812-13818	GO:0045177	denotes	apical
T8771	13819-13823	CL_GO_EXT:cell	denotes	cell
T8772	13819-13831	GO:0009986	denotes	cell surface
T8773	13837-13842	CHEBI:4450	denotes	dDAVP
T8774	13861-13870	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T8775	13946-13952	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T8776	13953-13960	CHEBI_PR_EXT:protein	denotes	protein
T8777	14046-14055	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T8778	14056-14063	CHEBI_PR_EXT:protein	denotes	protein
T8779	14078-14090	GO_PATO_EXT:biological_localization_process_or_quality	denotes	localization
T8780	14098-14104	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T8781	14105-14112	CHEBI_PR_EXT:protein	denotes	protein
T8782	14187-14196	GO_EXT:biological_routing_or_transport	denotes	transport
T8783	14203-14215	GO_MOP_EXT:glycosylation	denotes	glycosylated
T8784	14228-14232	PR_EXT:000004182	denotes	AQP2
T8785	14240-14244	CL_GO_EXT:cell	denotes	cell
T8786	14240-14252	GO:0009986	denotes	cell surface
T8787	14256-14268	GO_MOP_EXT:glycosylation	denotes	glycosylated
T8788	14312-14321	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T8789	14322-14326	PR_EXT:000004182	denotes	AQP2
T8790	14327-14334	CHEBI_PR_EXT:protein	denotes	protein
T8791	14339-14345	SO_EXT:sequence_rescue_process	denotes	rescue
T8792	14348-14361	GO_EXT:biological_translocation_entity_or_process	denotes	translocation
T8793	14372-14378	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T8794	14379-14386	CHEBI_PR_EXT:protein	denotes	protein
T8795	14388-14392	PR_EXT:000004182	denotes	AQP2
T8796	14419-14428	GO:0010467	denotes	expressed
T8797	14437-14442	CL_GO_EXT:cell	denotes	cells
T8798	16066-16082	UBERON:0001232	denotes	collecting ducts
T8799	16088-16092	PR_EXT:000004182	denotes	Aqp2
T8800	16098-16099	SO_EXT:normal_or_wild_type_or_present	denotes	+
T8801	16100-16104	NCBITaxon:10088	denotes	mice
T8802	16110-16119	SO_EXT:wild_type_entity_or_quality	denotes	wild type
T8803	16124-16130	SO_EXT:sequence_rescue_process	denotes	rescue
T8804	16135-16141	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T8805	16142-16149	CHEBI_PR_EXT:protein	denotes	protein
T8806	16170-16181	GO_UBERON_EXT:cellular_component_or_cell_part	denotes	subcellular
T8807	16173-16181	CL_GO_EXT:cell	denotes	cellular
T8808	16198-16202	PR_EXT:000004182	denotes	AQP2
T8809	16203-16210	CHEBI_PR_EXT:protein	denotes	protein
T8810	16274-16280	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T8811	16285-16294	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T8812	16295-16303	CHEBI_PR_EXT:protein	denotes	proteins
T8813	16307-16318	GO:0009294	denotes	transfected
T8814	16324-16329	CL_GO_EXT:cell	denotes	cells
T8815	16348-16353	CL_GO_EXT:cell	denotes	cells
T8816	16361-16371	GO:0010467	denotes	expressing
T8817	16372-16381	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T8818	16382-16386	PR_EXT:000004182	denotes	AQP2
T8819	16404-16415	GO:0009294	denotes	transfected
T8820	16425-16435	GO:0010467	denotes	expression
T8821	16436-16446	SO_EXT:engineered_biological_sequence	denotes	constructs
T8822	16447-16455	SO_EXT:sequence_coding_function	denotes	encoding
T8823	16460-16466	CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process	denotes	tagged
T8824	16467-16476	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T8825	16477-16481	PR_EXT:000004182	denotes	AQP2
T8826	16483-16487	PR_EXT:000004182	denotes	AQP2
T8827	16509-16519	GO:0042571	denotes	Antibodies
T8828	16553-16562	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T8829	16563-16567	PR_EXT:000004182	denotes	AQP2
T8830	16573-16577	PR_EXT:000004182	denotes	AQP2
T8831	16585-16589	PR_EXT:000004182	denotes	AQP2
T8832	16616-16627	GO:0009294	denotes	transfected
T8833	16672-16683	GO:0009294	denotes	transfected
T8834	16694-16699	CL_GO_EXT:cell	denotes	cells
T8835	16707-16717	GO:0010467	denotes	expressing
T8836	16718-16727	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T8837	16728-16732	PR_EXT:000004182	denotes	AQP2
T8838	16781-16790	GO:0016020	denotes	membranes
T8839	16803-16812	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T8840	16813-16817	PR_EXT:000004182	denotes	AQP2
T8841	16834-16843	GO:0010467	denotes	expressed
T8842	16892-16901	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T8843	16906-16912	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T8844	16913-16921	CHEBI_PR_EXT:protein	denotes	proteins
T8845	16952-16956	CL_GO_EXT:cell	denotes	cell
T8846	16992-16998	SO_EXT:sequence_rescue_process	denotes	rescue
T8847	17003-17015	GO_PATO_EXT:biological_localization_process_or_quality	denotes	localization
T8848	17019-17025	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T8849	17026-17033	CHEBI_PR_EXT:protein	denotes	protein
T8850	17073-17078	CL_GO_EXT:cell	denotes	cells
T8851	17086-17097	GO:0009294	denotes	transfected
T8852	17103-17112	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T8853	17113-17117	PR_EXT:000004182	denotes	AQP2
T8854	17118-17128	GO:0010467	denotes	expressing
T8855	17129-17135	SO_EXT:0000440	denotes	vector
T8856	17150-17156	SO_EXT:0000440	denotes	vector
T8857	17190-17201	GO:0009294	denotes	transfected
T8858	17202-17206	PR_EXT:000004182	denotes	AQP2
T8859	17214-17218	PR_EXT:000004182	denotes	AQP2
T8860	17229-17239	GO:0010467	denotes	expression
T8861	17240-17250	SO_EXT:engineered_biological_sequence	denotes	constructs
T8862	17252-17256	PR_EXT:000004182	denotes	AQP2
T8863	17261-17270	GO_PATO_EXT:biological_localization_process_or_quality	denotes	localized
T8864	17278-17284	GO:0045177	denotes	apical
T8865	17285-17292	GO:0009986	denotes	surface
T8866	17298-17307	CHEBI:42471	denotes	forskolin
T8867	17347-17358	GO:0009294	denotes	transfected
T8868	17364-17370	SO_EXT:0000440	denotes	vector
T8869	17376-17381	CL_GO_EXT:cell	denotes	cells
T8870	17421-17430	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T8871	17431-17435	PR_EXT:000004182	denotes	AQP2
T8872	17436-17441	CL_GO_EXT:cell	denotes	cells
T8873	17468-17472	PR_EXT:000004182	denotes	AQP2
T8874	17489-17498	GO:0010467	denotes	expressed
T8875	17512-17524	GO:0009294	denotes	transfection
T8876	17530-17536	SO_EXT:0000440	denotes	vector
T8877	17542-17547	CL_GO_EXT:cell	denotes	cells
T8878	17566-17577	GO:0005737	denotes	cytoplasmic
T8879	17629-17638	GO:0010467	denotes	expressed
T8880	17642-17651	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T8881	17652-17656	PR_EXT:000004182	denotes	AQP2
T8882	17657-17662	CL_GO_EXT:cell	denotes	cells
T8883	17673-17677	PR_EXT:000004182	denotes	AQP2
T8884	17688-17697	GO_PATO_EXT:biological_localization_process_or_quality	denotes	localized
T8885	17705-17711	GO:0045177	denotes	apical
T8886	17712-17719	GO:0009986	denotes	surface
T8887	17828-17833	CL_GO_EXT:cell	denotes	cells
T8888	17848-17860	GO:0009294	denotes	transfection
T8889	17880-17891	GO:0009294	denotes	transfected
T8890	17892-17896	CL_GO_EXT:cell	denotes	cell
T8891	17912-17924	GO_PATO_EXT:biological_localization_process_or_quality	denotes	localization
T8892	17939-17949	GO:0010467	denotes	expressing
T8893	17950-17959	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T8894	17960-17964	PR_EXT:000004182	denotes	AQP2
T8895	17975-17981	GO:0045177	denotes	apical
T8896	17987-17996	CHEBI:42471	denotes	forskolin
T8897	18028-18038	GO:0010467	denotes	expression
T8898	18058-18067	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T8899	18068-18072	PR_EXT:000004182	denotes	AQP2
T8900	18091-18095	PR_EXT:000004182	denotes	AQP2
T8901	18110-18114	CL_GO_EXT:cell	denotes	cell
T8902	18121-18133	GO_PATO_EXT:biological_localization_process_or_quality	denotes	localization
T8903	18137-18146	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T8904	18147-18151	PR_EXT:000004182	denotes	AQP2
T8905	18179-18183	PR_EXT:000004182	denotes	AQP2
T8906	18205-18211	GO:0045177	denotes	apical
T8907	18217-18226	CHEBI:42471	denotes	forskolin
T8908	18274-18278	CL_GO_EXT:cell	denotes	cell
T8909	18286-18290	PR_EXT:000004182	denotes	AQP2
T8910	18312-18321	GO_PATO_EXT:biological_localization_process_or_quality	denotes	localized
T8911	18329-18335	GO:0045177	denotes	apical
T8912	18336-18343	GO:0009986	denotes	surface
T8913	18360-18372	GO_PATO_EXT:biological_localization_process_or_quality	denotes	localization
T8914	18376-18380	PR_EXT:000004182	denotes	AQP2
T8915	18408-18414	GO:0045177	denotes	apical
T8916	18420-18429	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T8917	18430-18434	PR_EXT:000004182	denotes	AQP2
T8918	18444-18453	GO:0010467	denotes	expressed
T8919	18480-18493	CHEBI_EXT:biochemical	denotes	biochemically
T8920	18498-18509	GO:0009294	denotes	transfected
T8921	18519-18523	CL_GO_EXT:cell	denotes	cell
T8922	18531-18540	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T8923	18541-18545	PR_EXT:000004182	denotes	AQP2
T8924	18549-18555	SO_EXT:0000440	denotes	vector
T8925	18573-18585	MOP:0000093	denotes	biotinylated
T8926	18586-18593	GO:0009986	denotes	surface
T8927	18594-18602	CHEBI_PR_EXT:protein	denotes	proteins
T8928	18609-18618	CHEBI:42471	denotes	forskolin
T8929	18653-18665	MOP:0000093	denotes	biotinylated
T8930	18666-18674	CHEBI_PR_EXT:protein	denotes	proteins
T8931	18688-18692	PR_EXT:000004182	denotes	AQP2
T8932	18706-18715	GO:0010467	denotes	expressed
T8933	18746-18750	CL_GO_EXT:cell	denotes	cell
T8934	18775-18780	CL_GO_EXT:cell	denotes	cells
T8935	18790-18802	MOP:0000093	denotes	biotinylated
T8936	18813-18822	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T8937	18823-18827	PR_EXT:000004182	denotes	AQP2
T8938	18836-18845	GO:0010467	denotes	expressed
T8939	18858-18865	GO:0009986	denotes	surface
T8940	18866-18878	MOP:0000093	denotes	biotinylated
T8941	18892-18896	CL_GO_EXT:cell	denotes	cell
T8942	18892-18904	GO:0009986	denotes	cell surface
T8943	18905-18913	CHEBI_PR_EXT:protein	denotes	proteins
T8944	18932-18938	CHEBI:15956	denotes	biotin
T8945	18968-18972	PR_EXT:000004182	denotes	AQP2
T8946	18982-18993	GO_EXT:biological_routing_or_transport	denotes	transported
T8947	19001-19005	CL_GO_EXT:cell	denotes	cell
T8948	19001-19013	GO:0009986	denotes	cell surface
T8949	19019-19028	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T8950	19029-19033	PR_EXT:000004182	denotes	AQP2
T8524	3807-3812	CHEBI:4450	denotes	dDAVP

craft-ca-core-dev

Below, discontinuous spans are shown in the bag model. You can change it to the chain model.

Id	Subject	Object	Predicate	Lexical cue
T8093	46-62	CHEBI:23995	denotes	ethylnitrosourea
T8094	64-67	CHEBI:23995	denotes	ENU
T8095	102-108	NCBITaxon:33208	denotes	animal
T8096	168-178	GO:0008152	denotes	metabolism
T8097	209-213	NCBITaxon:10088	denotes	mice
T8098	219-227	GO:0060073	denotes	urinated
T8099	232-237	GO:0042756	denotes	drank
T8100	251-256	UBERON:0001977	denotes	Serum
T8101	261-266	UBERON:0001088	denotes	urine
T8102	288-294	UBERON:0001969	denotes	plasma
T8103	295-302	CHEBI:17234	denotes	glucose
T8104	339-346	CHEBI:17234	denotes	glucose
T8105	354-359	UBERON:0001088	denotes	urine
T8106	452-456	NCBITaxon:10088	denotes	mice
T8107	477-482	SO:0000704	denotes	genic
T8108	484-493	GO:0030849	denotes	autosomal
T8109	519-523	PR:000004182	denotes	Aqp2
T8110	536-540	SO:0000704	denotes	gene
T8111	556-560	PR:000004182	denotes	Aqp2
T8112	587-591	NCBITaxon:10088	denotes	mice
T8114	625-631,605-623,633-645	SO:1000022	denotes	T to G thymine to guanine transversion
T8116	1795-1799	PR:000004182	denotes	AQP2
T8117	1814-1822	GO:0016020	denotes	membrane
T8118	1823-1828	CHEBI:15377	denotes	water
T8119	1861-1874	GO:0005576	denotes	extracellular
T8120	1893-1901	GO:0016020	denotes	membrane
T8121	1911-1917	SO:0000417	denotes	domain
T8122	1980-1988	GO:0016020	denotes	membrane
T8123	1998-2005	SO:0000417	denotes	domains
T8124	2026-2036	NCBITaxon:7742	denotes	vertebrate
T8125	2037-2044	NCBITaxon:species	denotes	species
T8126	2139-2149	NCBITaxon:7742	denotes	vertebrate
T8127	2150-2154	PR:000004182	denotes	AQP2
T8128	2211-2215	PR:000004182	denotes	Aqp2
T8129	2227-2231	NCBITaxon:10088	denotes	mice
T8130	2260-2265	UBERON:0001088	denotes	urine
T8131	2315-2320	UBERON:0001088	denotes	urine
T8132	2431-2436	CHEBI:15377	denotes	water
T8133	2508-2513	CHEBI:15377	denotes	water
T8134	2514-2520	GO:0007631	denotes	intake
T8135	2537-2541	NCBITaxon:10088	denotes	mice
T8136	2575-2580	CHEBI:15377	denotes	water
T8137	2581-2587	GO:0007631	denotes	intake
T8138	2682-2689	UBERON:0001088	denotes	urinary
T8139	2701-2706	CHEBI:15377	denotes	water
T8140	2707-2713	GO:0007631	denotes	intake
T8141	2792-2799	NCBITaxon:33208	denotes	animals
T8142	2873-2878	UBERON:0001088	denotes	urine
T8143	2949-2953	PR:000004182	denotes	Aqp2
T8144	2965-2969	NCBITaxon:10088	denotes	mice
T8145	2990-2995	UBERON:0001088	denotes	urine
T8146	3015-3020	UBERON:0001088	denotes	urine
T8147	3045-3049	NCBITaxon:10088	denotes	mice
T8148	3111-3115	NCBITaxon:10088	denotes	mice
T8149	3139-3144	UBERON:0001088	denotes	urine
T8150	3172-3179	GO:0065007	denotes	control
T8151	3187-3199	UBERON:0001898	denotes	hypothalamus
T8152	3258-3266	GO:0046903	denotes	secretes
T8153	3298-3332	CHEBI:4450	denotes	1-deamino-8-D-arginine vasopressin
T8154	3334-3339	CHEBI:4450	denotes	dDAVP
T8155	3353-3365	CHEBI:4450	denotes	desmopressin
T8156	3391-3396	PR:000001683	denotes	AVPR2
T8157	3429-3433	NCBITaxon:10088	denotes	mice
T8158	3435-3440	CHEBI:4450	denotes	dDAVP
T8159	3473-3478	UBERON:0001088	denotes	urine
T8160	3573-3577	NCBITaxon:10088	denotes	mice
T8161	3596-3601	UBERON:0001088	denotes	urine
T8162	3699-3706	NCBITaxon:33208	denotes	animals
T8163	3748-3753	UBERON:0001088	denotes	urine
T8164	3807-3812	CHEBI:4450	denotes	dDAVP
T8165	3838-3843	CHEBI:4450	denotes	dDAVP
T8166	3891-3895	PR:000004182	denotes	AQP2
T8167	3955-3965	NCBITaxon:1	denotes	individual
T8168	4001-4006	NCBITaxon:10088	denotes	mouse
T8169	4035-4042	GO:0007618	denotes	matings
T8170	4055-4062	NCBITaxon:33208	denotes	animals
T8171	4296-4301	UBERON:0001088	denotes	urine
T8172	4317-4322	CHEBI:15377	denotes	water
T8173	4323-4329	GO:0007631	denotes	intake
T8174	4370-4374	NCBITaxon:10088	denotes	mice
T8175	4391-4398	UBERON:0002113	denotes	kidneys
T8176	4433-4440	NCBITaxon:33208	denotes	animals
T8177	4497-4501	NCBITaxon:10088	denotes	mice
T8178	4524-4532	UBERON:0000104	denotes	lifespan
T8179	4542-4548	NCBITaxon:33208	denotes	animal
T8180	4595-4602	NCBITaxon:33208	denotes	animals
T8181	5462-5466	PR:000004182	denotes	Aqp2
T8182	5478-5482	NCBITaxon:10088	denotes	mice
T8183	5635-5642	UBERON:0002113	denotes	kidneys
T8184	5650-5654	PR:000004182	denotes	Aqp2
T8185	5666-5670	NCBITaxon:10088	denotes	mice
T8186	5730-5734	NCBITaxon:10088	denotes	mice
T8187	5819-5823	PR:000004177	denotes	Aqp1
T8188	5824-5828	PR:000004183	denotes	Aqp3
T8189	5839-5843	NCBITaxon:10088	denotes	mice
T8190	5885-5889	PR:000004182	denotes	Aqp2
T8191	5901-5905	NCBITaxon:10088	denotes	mice
T8192	5951-5955	PR:000004182	denotes	Aqp2
T8193	5967-5971	NCBITaxon:10088	denotes	mice
T8194	6010-6022	UBERON:0001224	denotes	renal pelvis
T8195	6052-6058	UBERON:0000056	denotes	ureter
T8196	6098-6116	UBERON:0006660	denotes	muscularis propria
T8197	6205-6215	UBERON:0000365	denotes	urothelium
T8198	6226-6249	UBERON:0005910	denotes	transitional epithelium
T8199	6301-6307	UBERON:0002113	denotes	kidney
T8200	6325-6338	UBERON:0002015	denotes	renal capsule
T8201	6342-6354	UBERON:0001224	denotes	renal pelvis
T8202	6397-6406	UBERON:0000074	denotes	glomeruli
T8204	6411-6419,6427-6434	UBERON:0004134	denotes	proximal tubules
T8205	6420-6434	UBERON:0004135	denotes	distal tubules
T8206	6544-6548	PR:000004182	denotes	AQP2
T8207	6845-6852	CHEBI:37684	denotes	mannose
T8208	6870-6874	PR:000004182	denotes	AQP2
T8209	6957-6964	UBERON:0002113	denotes	kidneys
T8210	6978-6985	NCBITaxon:33208	denotes	animals
T8211	6987-6991	PR:000004182	denotes	AQP2
T8212	7004-7011	NCBITaxon:33208	denotes	animals
T8213	7191-7198	NCBITaxon:33208	denotes	animals
T8214	7355-7362	MOP:0000780	denotes	cleaves
T8215	7363-7370	CHEBI:37684	denotes	mannose
T8216	7376-7388	CHEBI:16646	denotes	carbohydrate
T8217	7440-7444	PR:000004182	denotes	AQP2
T8218	7450-7457	UBERON:0002113	denotes	kidneys
T8219	7497-7504	NCBITaxon:33208	denotes	animals
T8220	7644-7648	PR:000004182	denotes	Aqp2
T8221	7660-7664	NCBITaxon:10088	denotes	mice
T8222	7711-7715	PR:000004182	denotes	Aqp2
T8223	7727-7731	NCBITaxon:10088	denotes	mice
T8224	7781-7786	CHEBI:4450	denotes	dDAVP
T8225	8292-8298	NCBITaxon:9606	denotes	humans
T8226	8310-8317	SO:0001023	denotes	alleles
T8227	8321-8325	PR:000004182	denotes	Aqp2
T8228	8402-8408	GO:0045177	denotes	apical
T8229	8409-8421	GO:0009986	denotes	cell surface
T8230	8508-8512	PR:000004182	denotes	Aqp2
T8231	8536-8541	NCBITaxon:9606	denotes	human
T8232	8564-8575	GO:0009294	denotes	transfected
T8233	8581-8587	UBERON:0002113	denotes	kidney
T8234	8581-8592	CL:1000497	denotes	kidney cell
T8235	8627-8634	SO:0001023	denotes	alleles
T8236	8709-8717	GO:0031982	denotes	vesicles
T8237	8751-8772	GO:0005783	denotes	endoplasmic reticulum
T8238	8774-8776	GO:0005783	denotes	ER
T8239	8783-8788	NCBITaxon:10088	denotes	mouse
T8240	8868-8874	NCBITaxon:33208	denotes	animal
T8241	8929-8933	PR:000004182	denotes	AQP2
T8242	8977-8983	GO:0016324	denotes	apical
T8244	8984-8993,9010-9015	GO:0044464	denotes	region of cells
T8245	8994-9009	UBERON:0001232	denotes	collecting duct
T8246	8994-9015	CL:1001225	denotes	collecting duct cells
T8247	9019-9026	UBERON:0002113	denotes	kidneys
T8248	9040-9044	NCBITaxon:10088	denotes	mice
T8249	9068-9073	CHEBI:4450	denotes	dDAVP
T8250	9075-9079	PR:000004182	denotes	AQP2
T8251	9108-9120	GO:0009986	denotes	cell surface
T8252	9149-9156	UBERON:0002113	denotes	kidneys
T8253	9175-9182	NCBITaxon:33208	denotes	animals
T8254	9193-9197	PR:000004182	denotes	AQP2
T8255	9292-9296	PR:000004183	denotes	AQP3
T8256	9336-9347	GO:1990794	denotes	basolateral
T8257	9348-9355	GO:0009986	denotes	surface
T8258	9380-9385	CHEBI:4450	denotes	dDAVP
T8259	9399-9403	PR:000004182	denotes	AQP2
T8260	9439-9451	GO:0009986	denotes	cell surface
T8261	9533-9540	UBERON:0002113	denotes	kidneys
T8262	9564-9568	NCBITaxon:10088	denotes	mice
T8263	9622-9627	CHEBI:4450	denotes	dDAVP
T8264	11390-11394	PR:000004182	denotes	AQP2
T8265	11415-11427	GO:0009294	denotes	transfection
T8266	11461-11471	GO:0010467	denotes	expressing
T8267	11472-11477	NCBITaxon:10088	denotes	mouse
T8268	11488-11492	PR:000004182	denotes	AQP2
T8269	11497-11501	PR:000004182	denotes	AQP2
T8270	11657-11661	NCBITaxon:10088	denotes	mice
T8271	11754-11764	GO:0010467	denotes	expressing
T8272	11765-11769	PR:000004182	denotes	AQP2
T8273	11861-11872	GO:0009294	denotes	transfected
T8274	11909-11913	PR:000004182	denotes	AQP2
T8275	11991-11997	GO:0016324	denotes	apical
T8276	12064-12073	GO:0031982	denotes	vesicular
T8277	12096-12100	PR:000004182	denotes	AQP2
T8278	12158-12165	GO:0005634	denotes	nuclear
T8279	12223-12232	CHEBI:42471	denotes	forskolin
T8280	12236-12240	CHEBI:17489	denotes	cAMP
T8281	12287-12291	PR:000004182	denotes	AQP2
T8282	12312-12318	GO:0045177	denotes	apical
T8284	12319-12329,12345-12350	GO:0009986	denotes	surface of cells
T8285	12405-12412	GO:0005634	denotes	nuclear
T8286	12429-12433	PR:000004182	denotes	AQP2
T8287	12498-12507	CHEBI:42471	denotes	forskolin
T8288	12560-12567	GO:0005634	denotes	nuclear
T8289	12584-12588	PR:000004182	denotes	AQP2
T8290	12617-12619	GO:0005783	denotes	ER
T8291	12664-12668	PR:000004182	denotes	AQP2
T8292	12692-12694	GO:0005783	denotes	ER
T8293	12716-12727	GO:0009294	denotes	transfected
T8294	12733-12737	PR:000004182	denotes	Aqp2
T8295	12754-12758	PR:000004182	denotes	AQP2
T8296	12766-12768	GO:0005783	denotes	ER
T8297	12777-12785	PR:000005005	denotes	calnexin
T8298	12817-12825	PR:000005005	denotes	calnexin
T8299	12831-12835	PR:000004182	denotes	AQP2
T8300	12896-12900	PR:000004182	denotes	AQP2
T8301	12932-12940	PR:000005005	denotes	calnexin
T8302	12993-12995	GO:0005783	denotes	ER
T8303	13010-13014	PR:000004182	denotes	AQP2
T8304	13064-13066	GO:0005783	denotes	ER
T8305	13074-13076	GO:0005783	denotes	ER
T8306	13159-13163	PR:000004182	denotes	AQP2
T8307	13180-13187	UBERON:0002113	denotes	kidneys
T8308	13205-13212	NCBITaxon:33208	denotes	Animals
T8309	13234-13238	PR:000004182	denotes	Aqp2
T8310	13280-13285	UBERON:0001088	denotes	urine
T8311	13300-13305	UBERON:0001088	denotes	urine
T8312	13385-13391	SO:0001023	denotes	allele
T8313	13393-13397	PR:000004182	denotes	AQP2
T8314	13449-13456	CL:0000023	denotes	oocytes
T8315	13475-13491	GO:0051260	denotes	homo-oligomerize
T8316	13523-13530	UBERON:0002113	denotes	kidneys
T8317	13549-13556	NCBITaxon:33208	denotes	animals
T8318	13606-13610	PR:000004182	denotes	AQP2
T8319	13666-13689	UBERON:0001232	denotes	kidney collecting ducts
T8320	13695-13699	PR:000004182	denotes	Aqp2
T8321	13707-13711	NCBITaxon:10088	denotes	mice
T8322	13776-13780	PR:000004182	denotes	AQP2
T8323	13812-13818	GO:0045177	denotes	apical
T8324	13819-13831	GO:0009986	denotes	cell surface
T8325	13837-13842	CHEBI:4450	denotes	dDAVP
T8326	14228-14232	PR:000004182	denotes	AQP2
T8327	14240-14252	GO:0009986	denotes	cell surface
T8328	14322-14326	PR:000004182	denotes	AQP2
T8329	14388-14392	PR:000004182	denotes	AQP2
T8330	14419-14428	GO:0010467	denotes	expressed
T8331	16066-16082	UBERON:0001232	denotes	collecting ducts
T8332	16088-16092	PR:000004182	denotes	Aqp2
T8333	16100-16104	NCBITaxon:10088	denotes	mice
T8334	16198-16202	PR:000004182	denotes	AQP2
T8335	16307-16318	GO:0009294	denotes	transfected
T8336	16361-16371	GO:0010467	denotes	expressing
T8337	16382-16386	PR:000004182	denotes	AQP2
T8338	16404-16415	GO:0009294	denotes	transfected
T8339	16425-16435	GO:0010467	denotes	expression
T8340	16477-16481	PR:000004182	denotes	AQP2
T8341	16483-16487	PR:000004182	denotes	AQP2
T8342	16509-16519	GO:0042571	denotes	Antibodies
T8343	16563-16567	PR:000004182	denotes	AQP2
T8344	16573-16577	PR:000004182	denotes	AQP2
T8345	16585-16589	PR:000004182	denotes	AQP2
T8346	16616-16627	GO:0009294	denotes	transfected
T8347	16672-16683	GO:0009294	denotes	transfected
T8348	16707-16717	GO:0010467	denotes	expressing
T8349	16728-16732	PR:000004182	denotes	AQP2
T8350	16781-16790	GO:0016020	denotes	membranes
T8351	16813-16817	PR:000004182	denotes	AQP2
T8352	16834-16843	GO:0010467	denotes	expressed
T8353	17086-17097	GO:0009294	denotes	transfected
T8354	17113-17117	PR:000004182	denotes	AQP2
T8355	17118-17128	GO:0010467	denotes	expressing
T8356	17129-17135	SO:0000440	denotes	vector
T8357	17150-17156	SO:0000440	denotes	vector
T8358	17190-17201	GO:0009294	denotes	transfected
T8359	17202-17206	PR:000004182	denotes	AQP2
T8360	17214-17218	PR:000004182	denotes	AQP2
T8361	17229-17239	GO:0010467	denotes	expression
T8362	17252-17256	PR:000004182	denotes	AQP2
T8363	17278-17284	GO:0045177	denotes	apical
T8364	17285-17292	GO:0009986	denotes	surface
T8365	17298-17307	CHEBI:42471	denotes	forskolin
T8366	17347-17358	GO:0009294	denotes	transfected
T8367	17364-17370	SO:0000440	denotes	vector
T8368	17431-17435	PR:000004182	denotes	AQP2
T8369	17468-17472	PR:000004182	denotes	AQP2
T8370	17489-17498	GO:0010467	denotes	expressed
T8371	17512-17524	GO:0009294	denotes	transfection
T8372	17530-17536	SO:0000440	denotes	vector
T8373	17566-17577	GO:0005737	denotes	cytoplasmic
T8374	17629-17638	GO:0010467	denotes	expressed
T8375	17652-17656	PR:000004182	denotes	AQP2
T8376	17673-17677	PR:000004182	denotes	AQP2
T8377	17705-17711	GO:0045177	denotes	apical
T8378	17712-17719	GO:0009986	denotes	surface
T8379	17848-17860	GO:0009294	denotes	transfection
T8380	17880-17891	GO:0009294	denotes	transfected
T8381	17939-17949	GO:0010467	denotes	expressing
T8382	17960-17964	PR:000004182	denotes	AQP2
T8383	17975-17981	GO:0045177	denotes	apical
T8384	17987-17996	CHEBI:42471	denotes	forskolin
T8385	18028-18038	GO:0010467	denotes	expression
T8386	18068-18072	PR:000004182	denotes	AQP2
T8387	18091-18095	PR:000004182	denotes	AQP2
T8388	18147-18151	PR:000004182	denotes	AQP2
T8389	18179-18183	PR:000004182	denotes	AQP2
T8390	18205-18211	GO:0045177	denotes	apical
T8391	18217-18226	CHEBI:42471	denotes	forskolin
T8392	18286-18290	PR:000004182	denotes	AQP2
T8393	18329-18335	GO:0045177	denotes	apical
T8394	18336-18343	GO:0009986	denotes	surface
T8395	18376-18380	PR:000004182	denotes	AQP2
T8396	18408-18414	GO:0045177	denotes	apical
T8397	18430-18434	PR:000004182	denotes	AQP2
T8398	18444-18453	GO:0010467	denotes	expressed
T8399	18498-18509	GO:0009294	denotes	transfected
T8400	18541-18545	PR:000004182	denotes	AQP2
T8401	18549-18555	SO:0000440	denotes	vector
T8402	18573-18585	MOP:0000093	denotes	biotinylated
T8403	18586-18593	GO:0009986	denotes	surface
T8404	18609-18618	CHEBI:42471	denotes	forskolin
T8405	18653-18665	MOP:0000093	denotes	biotinylated
T8406	18688-18692	PR:000004182	denotes	AQP2
T8407	18706-18715	GO:0010467	denotes	expressed
T8408	18790-18802	MOP:0000093	denotes	biotinylated
T8409	18823-18827	PR:000004182	denotes	AQP2
T8410	18836-18845	GO:0010467	denotes	expressed
T8411	18858-18865	GO:0009986	denotes	surface
T8412	18866-18878	MOP:0000093	denotes	biotinylated
T8413	18892-18904	GO:0009986	denotes	cell surface
T8414	18932-18938	CHEBI:15956	denotes	biotin
T8415	18968-18972	PR:000004182	denotes	AQP2
T8416	19001-19013	GO:0009986	denotes	cell surface
T8417	19029-19033	PR:000004182	denotes	AQP2
T8092	21-28	SO:0000704	denotes	genetic

craft-sa-dev

Id	Subject	Object	Predicate	Lexical cue
T11284	17599-17600	-LRB-	denotes	(
T11285	17617-17621	JJ	denotes	left
T11286	17600-17606	NN	denotes	Figure
T11287	17607-17609	CD	denotes	5C
T11288	17609-17611	,	denotes	,
T11289	17611-17616	JJ	denotes	lower
T11290	17621-17622	-RRB-	denotes	)
T11291	17622-17623	.	denotes	.
T11292	17623-17779	sentence	denotes	When expressed in wild-type AQP2 cells, however, AQP2-F204V-GFP localized to the apical surface to varying degrees (Figure 5C, lower right images [i–iii]).
T8951	8-10	IN	denotes	In
T8952	191-196	VBD	denotes	found
T8953	11-12	DT	denotes	a
T8954	29-35	NN	denotes	screen
T8955	13-20	JJ	denotes	forward
T8956	21-28	JJ	denotes	genetic
T8957	36-40	WDT	denotes	that
T8958	41-45	VBD	denotes	used
T8959	46-62	NN	denotes	ethylnitrosourea
T8960	63-64	-LRB-	denotes	(
T8961	64-67	NN	denotes	ENU
T8962	67-68	-RRB-	denotes	)
T8963	69-71	TO	denotes	to
T8964	72-78	VB	denotes	induce
T8965	79-88	NNS	denotes	mutations
T8966	89-91	IN	denotes	in
T8967	92-93	DT	denotes	a
T8968	102-108	NN	denotes	animal
T8969	94-101	NN	denotes	founder
T8970	109-114	WP$	denotes	whose
T8971	115-124	NN	denotes	offspring
T8972	135-143	VBN	denotes	screened
T8973	125-129	VBD	denotes	were
T8974	130-134	RB	denotes	then
T8975	144-147	IN	denotes	for
T8976	148-156	JJ	denotes	abnormal
T8977	168-178	NN	denotes	metabolism
T8978	157-162	JJ	denotes	whole
T8979	163-167	NN	denotes	body
T8980	179-180	-LRB-	denotes	[
T8981	183-185	CD	denotes	20
T8982	180-182	CD	denotes	19
T8983	182-183	,	denotes	,
T8984	185-186	-RRB-	denotes	]
T8985	186-188	,	denotes	,
T8986	188-190	PRP	denotes	we
T8987	197-198	DT	denotes	a
T8988	199-205	NN	denotes	family
T8989	206-208	IN	denotes	of
T8990	209-213	NNS	denotes	mice
T8991	214-218	WDT	denotes	that
T8992	219-227	VBD	denotes	urinated
T8993	228-231	CC	denotes	and
T8994	232-237	VBD	denotes	drank
T8995	238-249	RB	denotes	excessively
T8996	249-250	.	denotes	.
T8997	250-379	sentence	denotes	Serum and urine analysis showed that plasma glucose levels were normal and there was no glucose in the urine (unpublished data).
T8998	251-256	NN	denotes	Serum
T8999	267-275	NN	denotes	analysis
T9000	257-260	CC	denotes	and
T9001	261-266	NN	denotes	urine
T9002	276-282	VBD	denotes	showed
T9003	283-287	IN	denotes	that
T9004	310-314	VBD	denotes	were
T9005	288-294	NN	denotes	plasma
T9006	303-309	NNS	denotes	levels
T9007	295-302	NN	denotes	glucose
T9008	315-321	JJ	denotes	normal
T9009	322-325	CC	denotes	and
T9010	326-331	EX	denotes	there
T9011	332-335	VBD	denotes	was
T9012	336-338	DT	denotes	no
T9013	339-346	NN	denotes	glucose
T9014	347-349	IN	denotes	in
T9015	350-353	DT	denotes	the
T9016	354-359	NN	denotes	urine
T9017	360-361	-LRB-	denotes	(
T9018	373-377	NNS	denotes	data
T9019	361-372	JJ	denotes	unpublished
T9020	377-378	-RRB-	denotes	)
T9021	378-379	.	denotes	.
T9022	379-429	sentence	denotes	Hence, this was an example of diabetes insipidus.
T9023	380-385	RB	denotes	Hence
T9024	392-395	VBD	denotes	was
T9025	385-387	,	denotes	,
T9026	387-391	DT	denotes	this
T9027	396-398	DT	denotes	an
T9028	399-406	NN	denotes	example
T9029	407-409	IN	denotes	of
T9030	410-418	NN	denotes	diabetes
T9031	419-428	NN	denotes	insipidus
T9032	428-429	.	denotes	.
T9033	429-541	sentence	denotes	The disorder in these mice segregated in a monogenic, autosomal recessive manner, making Aqp2 a candidate gene.
T9034	430-433	DT	denotes	The
T9035	434-442	NN	denotes	disorder
T9036	457-467	VBD	denotes	segregated
T9037	443-445	IN	denotes	in
T9038	446-451	DT	denotes	these
T9039	452-456	NNS	denotes	mice
T9040	468-470	IN	denotes	in
T9041	471-472	DT	denotes	a
T9042	504-510	NN	denotes	manner
T9043	473-482	JJ	denotes	monogenic
T9044	482-484	,	denotes	,
T9045	484-493	JJ	denotes	autosomal
T9046	494-503	JJ	denotes	recessive
T9047	510-512	,	denotes	,
T9048	512-518	VBG	denotes	making
T9049	519-523	NN	denotes	Aqp2
T9050	536-540	NN	denotes	gene
T9051	524-525	DT	denotes	a
T9052	526-535	NN	denotes	candidate
T9053	540-541	.	denotes	.
T9054	541-770	sentence	denotes	Sequencing of Aqp2 coding region of affected mice identified a thymine to guanine (T to G) transversion (Figure 1A), which is predicted to lead to a valine for phenylalanine substitution at amino acid 204 of the protein (F204V).
T9055	542-552	NN	denotes	Sequencing
T9056	592-602	VBD	denotes	identified
T9057	553-555	IN	denotes	of
T9058	556-560	NN	denotes	Aqp2
T9059	568-574	NN	denotes	region
T9060	561-567	NN	denotes	coding
T9061	575-577	IN	denotes	of
T9062	578-586	VBN	denotes	affected
T9063	587-591	NNS	denotes	mice
T9064	603-604	DT	denotes	a
T9065	633-645	NN	denotes	transversion
T9066	605-612	NN	denotes	thymine
T9067	613-615	IN	denotes	to
T9068	616-623	NN	denotes	guanine
T9069	624-625	-LRB-	denotes	(
T9070	625-626	NN	denotes	T
T9071	627-629	IN	denotes	to
T9072	630-631	NN	denotes	G
T9073	631-632	-RRB-	denotes	)
T9074	646-647	-LRB-	denotes	(
T9075	647-653	NN	denotes	Figure
T9076	654-656	CD	denotes	1A
T9077	656-657	-RRB-	denotes	)
T9078	657-659	,	denotes	,
T9079	659-664	WDT	denotes	which
T9080	668-677	VBN	denotes	predicted
T9081	665-667	VBZ	denotes	is
T9082	678-680	TO	denotes	to
T9083	681-685	VB	denotes	lead
T9084	686-688	IN	denotes	to
T9085	689-690	DT	denotes	a
T9086	716-728	NN	denotes	substitution
T9087	691-697	NN	denotes	valine
T9088	698-701	IN	denotes	for
T9089	702-715	NN	denotes	phenylalanine
T9090	729-731	IN	denotes	at
T9091	732-737	NN	denotes	amino
T9092	738-742	NN	denotes	acid
T9093	743-746	CD	denotes	204
T9094	747-749	IN	denotes	of
T9095	750-753	DT	denotes	the
T9096	754-761	NN	denotes	protein
T9097	762-763	-LRB-	denotes	(
T9098	763-768	NN	denotes	F204V
T9099	768-769	-RRB-	denotes	)
T9100	769-770	.	denotes	.
T9101	770-1960	sentence	denotes	Figure 1 Analysis of Aqp2 Sequence and Phenotype in Mutant Mice (A) Chromatographic traces of Aqp2 F204V mutation. The box shows the mutated codon, TTC (Phe) to GTC (Val) at position 204. WT, wild type; Mut, mutant. (B) Amino acid conservation of mouse AQP2 (residues 194–214). The boxed residue indicates phenylalanine at position 204. hAQP2, human AQP2; mAQP1, mouse AQP1; mAQP2, mouse AQP2; rAQP2, rat AQP2; xAQP2, Xenopus AQP2. (C) Urine production (ml) and water consumption (ml) of 58 F2 mice over a 24-h period (both sexes, aged 10–22 wk). Mutant mice (black squares) exhibit overt polyuria and polydipsia compared to littermate wild-type (white triangles) and heterozygous (grey circles) mice. (D) Urine osmolality and concentrating ability in Aqp2 mutant and their littermates (10–22 wk, both sexes), before (white bars) and after (black bars) dDAVP treatment. Wild type (WT; n = 12); heterozygote (Het; n = 20); mutant (Mut; n = 9). Data represent averages ± standard error of the mean, p < 0.01; *p < 0.001. AQP2 is a six-transmembrane water channel, and F204 lies near the extracellular face of the sixth membrane spanning domain, a region rich in hydrophobic amino acids.
T9102	1795-1799	NN	denotes	AQP2
T9103	1800-1802	VBZ	denotes	is
T9104	1803-1804	DT	denotes	a
T9105	1829-1836	NN	denotes	channel
T9106	1805-1808	CD	denotes	six
T9107	1809-1822	NN	denotes	transmembrane
T9108	1808-1809	HYPH	denotes	-
T9109	1823-1828	NN	denotes	water
T9110	1836-1838	,	denotes	,
T9111	1838-1841	CC	denotes	and
T9112	1842-1846	NN	denotes	F204
T9113	1847-1851	VBZ	denotes	lies
T9114	1852-1856	IN	denotes	near
T9115	1857-1860	DT	denotes	the
T9116	1875-1879	NN	denotes	face
T9117	1861-1874	JJ	denotes	extracellular
T9118	1880-1882	IN	denotes	of
T9119	1883-1886	DT	denotes	the
T9120	1911-1917	NN	denotes	domain
T9121	1887-1892	JJ	denotes	sixth
T9122	1893-1901	NN	denotes	membrane
T9123	1902-1910	VBG	denotes	spanning
T9124	1917-1919	,	denotes	,
T9125	1919-1920	DT	denotes	a
T9126	1921-1927	NN	denotes	region
T9127	1928-1932	JJ	denotes	rich
T9128	1933-1935	IN	denotes	in
T9129	1936-1947	JJ	denotes	hydrophobic
T9130	1954-1959	NNS	denotes	acids
T9131	1948-1953	NN	denotes	amino
T9132	1959-1960	.	denotes	.
T9133	1960-2045	sentence	denotes	This and the other membrane-spanning domains are conserved among vertebrate species.
T9134	1961-1965	DT	denotes	This
T9135	2010-2019	VBN	denotes	conserved
T9136	1966-1969	CC	denotes	and
T9137	1970-1973	DT	denotes	the
T9138	1974-1979	JJ	denotes	other
T9139	1998-2005	NNS	denotes	domains
T9140	1980-1988	NN	denotes	membrane
T9141	1989-1997	VBG	denotes	spanning
T9142	1988-1989	HYPH	denotes	-
T9143	2006-2009	VBP	denotes	are
T9144	2020-2025	IN	denotes	among
T9145	2026-2036	JJ	denotes	vertebrate
T9146	2037-2044	NNS	denotes	species
T9147	2044-2045	.	denotes	.
T9148	2045-2210	sentence	denotes	The phenylalanine at position 204 is particularly well conserved (Figure 1B), not only among vertebrate AQP2 proteins, but also among others members of this family.
T9149	2046-2049	DT	denotes	The
T9150	2050-2063	NN	denotes	phenylalanine
T9151	2080-2082	VBZ	denotes	is
T9152	2064-2066	IN	denotes	at
T9153	2067-2075	NN	denotes	position
T9154	2076-2079	CD	denotes	204
T9155	2083-2095	RB	denotes	particularly
T9156	2101-2110	VBN	denotes	conserved
T9157	2096-2100	RB	denotes	well
T9158	2111-2112	-LRB-	denotes	(
T9159	2112-2118	NN	denotes	Figure
T9160	2119-2121	CD	denotes	1B
T9161	2121-2122	-RRB-	denotes	)
T9162	2122-2124	,	denotes	,
T9163	2124-2127	RB	denotes	not
T9164	2133-2138	IN	denotes	among
T9165	2128-2132	RB	denotes	only
T9166	2139-2149	JJ	denotes	vertebrate
T9167	2155-2163	NN	denotes	proteins
T9168	2150-2154	NN	denotes	AQP2
T9169	2163-2165	,	denotes	,
T9170	2165-2168	CC	denotes	but
T9171	2169-2173	RB	denotes	also
T9172	2174-2179	IN	denotes	among
T9173	2180-2186	NNS	denotes	others
T9174	2187-2194	NNS	denotes	members
T9175	2195-2197	IN	denotes	of
T9176	2198-2202	DT	denotes	this
T9177	2203-2209	NN	denotes	family
T9178	2209-2210	.	denotes	.
T9179	2210-2417	sentence	denotes	Aqp2F204V/F204V mice have dramatically increased urine production, in some cases producing an amount of urine in 24 h that exceeds their body weight, compared to their heterozygous or wild-type littermates.
T9180	2211-2220	NN	denotes	Aqp2F204V
T9181	2221-2226	NN	denotes	F204V
T9182	2220-2221	HYPH	denotes	/
T9183	2227-2231	NNS	denotes	mice
T9184	2232-2236	VBP	denotes	have
T9185	2237-2249	RB	denotes	dramatically
T9186	2250-2259	VBN	denotes	increased
T9187	2266-2276	NN	denotes	production
T9188	2260-2265	NN	denotes	urine
T9189	2276-2278	,	denotes	,
T9190	2278-2280	IN	denotes	in
T9191	2292-2301	VBG	denotes	producing
T9192	2281-2285	DT	denotes	some
T9193	2286-2291	NNS	denotes	cases
T9194	2302-2304	DT	denotes	an
T9195	2305-2311	NN	denotes	amount
T9196	2312-2314	IN	denotes	of
T9197	2315-2320	NN	denotes	urine
T9198	2321-2323	IN	denotes	in
T9199	2324-2326	CD	denotes	24
T9200	2327-2328	NN	denotes	h
T9201	2329-2333	WDT	denotes	that
T9202	2334-2341	VBZ	denotes	exceeds
T9203	2342-2347	PRP$	denotes	their
T9204	2353-2359	NN	denotes	weight
T9205	2348-2352	NN	denotes	body
T9206	2359-2361	,	denotes	,
T9207	2361-2369	VBN	denotes	compared
T9208	2370-2372	IN	denotes	to
T9209	2373-2378	PRP$	denotes	their
T9210	2405-2416	NNS	denotes	littermates
T9211	2379-2391	JJ	denotes	heterozygous
T9212	2392-2394	CC	denotes	or
T9213	2395-2399	JJ	denotes	wild
T9214	2400-2404	NN	denotes	type
T9215	2399-2400	HYPH	denotes	-
T9216	2416-2417	.	denotes	.
T9217	2417-2521	sentence	denotes	Such loss of water would rapidly lead to dehydration were it not compensated by increased water intake.
T9218	2418-2422	JJ	denotes	Such
T9219	2423-2427	NN	denotes	loss
T9220	2451-2455	VB	denotes	lead
T9221	2428-2430	IN	denotes	of
T9222	2431-2436	NN	denotes	water
T9223	2437-2442	MD	denotes	would
T9224	2443-2450	RB	denotes	rapidly
T9225	2456-2458	IN	denotes	to
T9226	2459-2470	NN	denotes	dehydration
T9227	2471-2475	VBD	denotes	were
T9228	2483-2494	VBN	denotes	compensated
T9229	2476-2478	PRP	denotes	it
T9230	2479-2482	RB	denotes	not
T9231	2495-2497	IN	denotes	by
T9232	2498-2507	VBN	denotes	increased
T9233	2514-2520	NN	denotes	intake
T9234	2508-2513	NN	denotes	water
T9235	2520-2521	.	denotes	.
T9236	2521-2656	sentence	denotes	Indeed, mutant mice also dramatically increase their water intake (Figure 1C) compared to their heterozygous or wild-type littermates.
T9237	2522-2528	RB	denotes	Indeed
T9238	2560-2568	VB	denotes	increase
T9239	2528-2530	,	denotes	,
T9240	2530-2536	NN	denotes	mutant
T9241	2537-2541	NNS	denotes	mice
T9242	2542-2546	RB	denotes	also
T9243	2547-2559	RB	denotes	dramatically
T9244	2569-2574	PRP$	denotes	their
T9245	2581-2587	NN	denotes	intake
T9246	2575-2580	NN	denotes	water
T9247	2588-2589	-LRB-	denotes	(
T9248	2589-2595	NN	denotes	Figure
T9249	2596-2598	CD	denotes	1C
T9250	2598-2599	-RRB-	denotes	)
T9251	2600-2608	VBN	denotes	compared
T9252	2609-2611	IN	denotes	to
T9253	2612-2617	PRP$	denotes	their
T9254	2644-2655	NNS	denotes	littermates
T9255	2618-2630	JJ	denotes	heterozygous
T9256	2631-2633	CC	denotes	or
T9257	2634-2638	JJ	denotes	wild
T9258	2639-2643	NN	denotes	type
T9259	2638-2639	HYPH	denotes	-
T9260	2655-2656	.	denotes	.
T9261	2656-2807	sentence	denotes	This phenotype—increased urinary output and water intake—showed complete concordance with homozygosity of the F204V mutation in the 58 animals tested.
T9262	2657-2661	DT	denotes	This
T9263	2662-2671	NN	denotes	phenotype
T9264	2714-2720	VBD	denotes	showed
T9265	2671-2672	,	denotes	—
T9266	2672-2681	VBN	denotes	increased
T9267	2690-2696	NN	denotes	output
T9268	2682-2689	JJ	denotes	urinary
T9269	2697-2700	CC	denotes	and
T9270	2701-2706	NN	denotes	water
T9271	2707-2713	NN	denotes	intake
T9272	2713-2714	,	denotes	—
T9273	2721-2729	JJ	denotes	complete
T9274	2730-2741	NN	denotes	concordance
T9275	2742-2746	IN	denotes	with
T9276	2747-2759	NN	denotes	homozygosity
T9277	2760-2762	IN	denotes	of
T9278	2763-2766	DT	denotes	the
T9279	2773-2781	NN	denotes	mutation
T9280	2767-2772	NN	denotes	F204V
T9281	2782-2784	IN	denotes	in
T9282	2785-2788	DT	denotes	the
T9283	2792-2799	NNS	denotes	animals
T9284	2789-2791	CD	denotes	58
T9285	2800-2806	VBN	denotes	tested
T9286	2806-2807	.	denotes	.
T9287	2807-2897	sentence	denotes	Diabetes insipidus can be defined as an inability to concentrate urine where appropriate.
T9288	2808-2816	NN	denotes	Diabetes
T9289	2817-2826	NN	denotes	insipidus
T9290	2834-2841	VBN	denotes	defined
T9291	2827-2830	MD	denotes	can
T9292	2831-2833	VB	denotes	be
T9293	2842-2844	IN	denotes	as
T9294	2845-2847	DT	denotes	an
T9295	2848-2857	NN	denotes	inability
T9296	2858-2860	TO	denotes	to
T9297	2861-2872	VB	denotes	concentrate
T9298	2873-2878	NN	denotes	urine
T9299	2879-2884	WRB	denotes	where
T9300	2885-2896	JJ	denotes	appropriate
T9301	2896-2897	.	denotes	.
T9302	2897-3008	sentence	denotes	Compared to wild-type or heterozygous littermates, Aqp2F204V/F204V mice produce very dilute urine (Figure 1D).
T9303	2898-2906	VBN	denotes	Compared
T9304	2970-2977	VBP	denotes	produce
T9305	2907-2909	IN	denotes	to
T9306	2910-2914	JJ	denotes	wild
T9307	2915-2919	NN	denotes	type
T9308	2914-2915	HYPH	denotes	-
T9309	2936-2947	NNS	denotes	littermates
T9310	2920-2922	CC	denotes	or
T9311	2923-2935	JJ	denotes	heterozygous
T9312	2947-2949	,	denotes	,
T9313	2949-2958	NN	denotes	Aqp2F204V
T9314	2959-2964	NN	denotes	F204V
T9315	2958-2959	HYPH	denotes	/
T9316	2965-2969	NNS	denotes	mice
T9317	2978-2982	RB	denotes	very
T9318	2983-2989	JJ	denotes	dilute
T9319	2990-2995	NN	denotes	urine
T9320	2996-2997	-LRB-	denotes	(
T9321	2997-3003	NN	denotes	Figure
T9322	3004-3006	CD	denotes	1D
T9323	3006-3007	-RRB-	denotes	)
T9324	3007-3008	.	denotes	.
T9325	3008-3128	sentence	denotes	Basal urine concentration in mutant mice is about 161 mOsm, compared to about 1,293 mOsm in wild-type mice (p < 0.001).
T9326	3009-3014	JJ	denotes	Basal
T9327	3021-3034	NN	denotes	concentration
T9328	3015-3020	NN	denotes	urine
T9329	3050-3052	VBZ	denotes	is
T9330	3035-3037	IN	denotes	in
T9331	3038-3044	NN	denotes	mutant
T9332	3045-3049	NNS	denotes	mice
T9333	3053-3058	RB	denotes	about
T9334	3059-3062	CD	denotes	161
T9335	3063-3067	NN	denotes	mOsm
T9336	3067-3069	,	denotes	,
T9337	3069-3077	VBN	denotes	compared
T9338	3078-3080	IN	denotes	to
T9339	3081-3086	IN	denotes	about
T9340	3087-3092	CD	denotes	1,293
T9341	3093-3097	NN	denotes	mOsm
T9342	3098-3100	IN	denotes	in
T9343	3101-3105	JJ	denotes	wild
T9344	3106-3110	NN	denotes	type
T9345	3105-3106	HYPH	denotes	-
T9346	3111-3115	NNS	denotes	mice
T9347	3116-3117	-LRB-	denotes	(
T9348	3121-3126	CD	denotes	0.001
T9349	3117-3118	NN	denotes	p
T9350	3119-3120	SYM	denotes	<
T9351	3126-3127	-RRB-	denotes	)
T9352	3127-3128	.	denotes	.
T9353	3128-3271	sentence	denotes	Normally, urine concentration is under the control of the hypothalamus, which, in response to hypovolemia or hypernatremia [21], secretes AVP.
T9354	3129-3137	RB	denotes	Normally
T9355	3159-3161	VBZ	denotes	is
T9356	3137-3139	,	denotes	,
T9357	3139-3144	NN	denotes	urine
T9358	3145-3158	NN	denotes	concentration
T9359	3162-3167	IN	denotes	under
T9360	3168-3171	DT	denotes	the
T9361	3172-3179	NN	denotes	control
T9362	3180-3182	IN	denotes	of
T9363	3183-3186	DT	denotes	the
T9364	3187-3199	NN	denotes	hypothalamus
T9365	3199-3201	,	denotes	,
T9366	3201-3206	WDT	denotes	which
T9367	3258-3266	VBZ	denotes	secretes
T9368	3206-3208	,	denotes	,
T9369	3208-3210	IN	denotes	in
T9370	3211-3219	NN	denotes	response
T9371	3220-3222	IN	denotes	to
T9372	3223-3234	NN	denotes	hypovolemia
T9373	3235-3237	CC	denotes	or
T9374	3238-3251	NN	denotes	hypernatremia
T9375	3252-3253	-LRB-	denotes	[
T9376	3253-3255	CD	denotes	21
T9377	3255-3256	-RRB-	denotes	]
T9378	3256-3258	,	denotes	,
T9379	3267-3270	NN	denotes	AVP
T9380	3270-3271	.	denotes	.
T9381	3271-3397	sentence	denotes	The synthetic AVP analog, 1-deamino-8-D-arginine vasopressin (dDAVP; also called desmopressin), is a potent agonist of AVPR2.
T9382	3272-3275	DT	denotes	The
T9383	3290-3296	NN	denotes	analog
T9384	3276-3285	JJ	denotes	synthetic
T9385	3286-3289	NN	denotes	AVP
T9386	3368-3370	VBZ	denotes	is
T9387	3296-3298	,	denotes	,
T9388	3298-3299	CD	denotes	1
T9389	3300-3307	NN	denotes	deamino
T9390	3299-3300	HYPH	denotes	-
T9391	3312-3320	NN	denotes	arginine
T9392	3307-3308	HYPH	denotes	-
T9393	3308-3309	CD	denotes	8
T9394	3309-3310	HYPH	denotes	-
T9395	3310-3311	NN	denotes	D
T9396	3311-3312	HYPH	denotes	-
T9397	3321-3332	NN	denotes	vasopressin
T9398	3333-3334	-LRB-	denotes	(
T9399	3334-3339	NN	denotes	dDAVP
T9400	3339-3340	:	denotes	;
T9401	3341-3345	RB	denotes	also
T9402	3346-3352	VBN	denotes	called
T9403	3353-3365	NN	denotes	desmopressin
T9404	3365-3366	-RRB-	denotes	)
T9405	3366-3368	,	denotes	,
T9406	3371-3372	DT	denotes	a
T9407	3380-3387	NN	denotes	agonist
T9408	3373-3379	JJ	denotes	potent
T9409	3388-3390	IN	denotes	of
T9410	3391-3396	NN	denotes	AVPR2
T9411	3396-3397	.	denotes	.
T9412	3397-3541	sentence	denotes	When administered to wild-type mice, dDAVP leads to a dramatic increase in urine concentration, from 1,293 to 5,885 mOsm (4.6-fold; Figure 1D).
T9413	3398-3402	WRB	denotes	When
T9414	3403-3415	VBN	denotes	administered
T9415	3441-3446	VBZ	denotes	leads
T9416	3416-3418	IN	denotes	to
T9417	3419-3423	JJ	denotes	wild
T9418	3424-3428	NN	denotes	type
T9419	3423-3424	HYPH	denotes	-
T9420	3429-3433	NNS	denotes	mice
T9421	3433-3435	,	denotes	,
T9422	3435-3440	NN	denotes	dDAVP
T9423	3447-3449	IN	denotes	to
T9424	3450-3451	DT	denotes	a
T9425	3461-3469	NN	denotes	increase
T9426	3452-3460	JJ	denotes	dramatic
T9427	3470-3472	IN	denotes	in
T9428	3473-3478	NN	denotes	urine
T9429	3479-3492	NN	denotes	concentration
T9430	3492-3494	,	denotes	,
T9431	3494-3498	IN	denotes	from
T9432	3499-3504	CD	denotes	1,293
T9433	3505-3507	IN	denotes	to
T9434	3508-3513	CD	denotes	5,885
T9435	3514-3518	NN	denotes	mOsm
T9436	3519-3520	-LRB-	denotes	(
T9437	3530-3536	NN	denotes	Figure
T9438	3520-3528	RB	denotes	4.6-fold
T9439	3528-3529	:	denotes	;
T9440	3537-3539	CD	denotes	1D
T9441	3539-3540	-RRB-	denotes	)
T9442	3540-3541	.	denotes	.
T9443	3541-3813	sentence	denotes	With similar treatment, mutant mice concentrate their urine to a lesser but still significant extent, from 161 to 470 mOsm (2.9-fold), indicating that these animals are not only unable to concentrate their urine properly but are also defective in their response to dDAVP.
T9444	3542-3546	IN	denotes	With
T9445	3578-3589	VBP	denotes	concentrate
T9446	3547-3554	JJ	denotes	similar
T9447	3555-3564	NN	denotes	treatment
T9448	3564-3566	,	denotes	,
T9449	3566-3572	NN	denotes	mutant
T9450	3573-3577	NNS	denotes	mice
T9451	3590-3595	PRP$	denotes	their
T9452	3596-3601	NN	denotes	urine
T9453	3602-3604	IN	denotes	to
T9454	3605-3606	DT	denotes	a
T9455	3636-3642	NN	denotes	extent
T9456	3607-3613	JJR	denotes	lesser
T9457	3614-3617	CC	denotes	but
T9458	3618-3623	RB	denotes	still
T9459	3624-3635	JJ	denotes	significant
T9460	3642-3644	,	denotes	,
T9461	3644-3648	IN	denotes	from
T9462	3649-3652	CD	denotes	161
T9463	3653-3655	IN	denotes	to
T9464	3656-3659	CD	denotes	470
T9465	3660-3664	NN	denotes	mOsm
T9466	3665-3666	-LRB-	denotes	(
T9467	3666-3674	RB	denotes	2.9-fold
T9468	3674-3675	-RRB-	denotes	)
T9469	3675-3677	,	denotes	,
T9470	3677-3687	VBG	denotes	indicating
T9471	3688-3692	IN	denotes	that
T9472	3707-3710	VBP	denotes	are
T9473	3693-3698	DT	denotes	these
T9474	3699-3706	NNS	denotes	animals
T9475	3711-3714	RB	denotes	not
T9476	3715-3719	RB	denotes	only
T9477	3720-3726	JJ	denotes	unable
T9478	3727-3729	TO	denotes	to
T9479	3730-3741	VB	denotes	concentrate
T9480	3742-3747	PRP$	denotes	their
T9481	3748-3753	NN	denotes	urine
T9482	3754-3762	RB	denotes	properly
T9483	3763-3766	CC	denotes	but
T9484	3767-3770	VBP	denotes	are
T9485	3771-3775	RB	denotes	also
T9486	3776-3785	JJ	denotes	defective
T9487	3786-3788	IN	denotes	in
T9488	3789-3794	PRP$	denotes	their
T9489	3795-3803	NN	denotes	response
T9490	3804-3806	IN	denotes	to
T9491	3807-3812	NN	denotes	dDAVP
T9492	3812-3813	.	denotes	.
T9493	3813-4012	sentence	denotes	The smaller response to dDAVP indicates some residual activity of the mutant AQP2 channel, which must be sufficient to allow survival of the individual, in contrast to the T126M knock-in mouse [18].
T9494	3814-3817	DT	denotes	The
T9495	3826-3834	NN	denotes	response
T9496	3818-3825	JJR	denotes	smaller
T9497	3844-3853	VBZ	denotes	indicates
T9498	3835-3837	IN	denotes	to
T9499	3838-3843	NN	denotes	dDAVP
T9500	3854-3858	DT	denotes	some
T9501	3868-3876	NN	denotes	activity
T9502	3859-3867	JJ	denotes	residual
T9503	3877-3879	IN	denotes	of
T9504	3880-3883	DT	denotes	the
T9505	3896-3903	NN	denotes	channel
T9506	3884-3890	NN	denotes	mutant
T9507	3891-3895	NN	denotes	AQP2
T9508	3903-3905	,	denotes	,
T9509	3905-3910	WDT	denotes	which
T9510	3916-3918	VB	denotes	be
T9511	3911-3915	MD	denotes	must
T9512	3919-3929	JJ	denotes	sufficient
T9513	3930-3932	TO	denotes	to
T9514	3933-3938	VB	denotes	allow
T9515	3939-3947	NN	denotes	survival
T9516	3948-3950	IN	denotes	of
T9517	3951-3954	DT	denotes	the
T9518	3955-3965	NN	denotes	individual
T9519	3965-3967	,	denotes	,
T9520	3967-3969	IN	denotes	in
T9521	3970-3978	NN	denotes	contrast
T9522	3979-3981	IN	denotes	to
T9523	3982-3985	DT	denotes	the
T9524	4001-4006	NN	denotes	mouse
T9525	3986-3991	NN	denotes	T126M
T9526	3992-3997	VB	denotes	knock
T9527	3997-3998	HYPH	denotes	-
T9528	3998-4000	RP	denotes	in
T9529	4007-4008	-LRB-	denotes	[
T9530	4008-4010	CD	denotes	18
T9531	4010-4011	-RRB-	denotes	]
T9532	4011-4012	.	denotes	.
T9533	4012-4270	sentence	denotes	Multiple heterozygous matings yielded 101 animals, which appeared at a ratio of 26:49:26, near the expected Mendelian wild type, heterozygote, and mutant frequencies, respectively, indicating that there is no reduced viability associated with this mutation.
T9534	4013-4021	JJ	denotes	Multiple
T9535	4035-4042	NNS	denotes	matings
T9536	4022-4034	JJ	denotes	heterozygous
T9537	4043-4050	VBD	denotes	yielded
T9538	4051-4054	CD	denotes	101
T9539	4055-4062	NNS	denotes	animals
T9540	4062-4064	,	denotes	,
T9541	4064-4069	WDT	denotes	which
T9542	4070-4078	VBD	denotes	appeared
T9543	4079-4081	IN	denotes	at
T9544	4082-4083	DT	denotes	a
T9545	4084-4089	NN	denotes	ratio
T9546	4090-4092	IN	denotes	of
T9547	4093-4095	CD	denotes	26
T9548	4095-4096	SYM	denotes	:
T9549	4096-4098	CD	denotes	49
T9550	4098-4099	SYM	denotes	:
T9551	4099-4101	CD	denotes	26
T9552	4101-4103	,	denotes	,
T9553	4103-4107	IN	denotes	near
T9554	4108-4111	DT	denotes	the
T9555	4167-4178	NNS	denotes	frequencies
T9556	4112-4120	VBN	denotes	expected
T9557	4121-4130	JJ	denotes	Mendelian
T9558	4136-4140	NN	denotes	type
T9559	4131-4135	JJ	denotes	wild
T9560	4140-4142	,	denotes	,
T9561	4142-4154	NN	denotes	heterozygote
T9562	4154-4156	,	denotes	,
T9563	4156-4159	CC	denotes	and
T9564	4160-4166	NN	denotes	mutant
T9565	4178-4180	,	denotes	,
T9566	4180-4192	RB	denotes	respectively
T9567	4192-4194	,	denotes	,
T9568	4194-4204	VBG	denotes	indicating
T9569	4205-4209	IN	denotes	that
T9570	4216-4218	VBZ	denotes	is
T9571	4210-4215	EX	denotes	there
T9572	4219-4221	DT	denotes	no
T9573	4230-4239	NN	denotes	viability
T9574	4222-4229	VBN	denotes	reduced
T9575	4240-4250	VBN	denotes	associated
T9576	4251-4255	IN	denotes	with
T9577	4256-4260	DT	denotes	this
T9578	4261-4269	NN	denotes	mutation
T9579	4269-4270	.	denotes	.
T9580	4270-4453	sentence	denotes	Other than the increased urine production and water intake, there was no overt phenotype in mutant mice, save distended kidneys, which appeared variably in adult animals (Figure 2A).
T9581	4271-4276	JJ	denotes	Other
T9582	4337-4340	VBD	denotes	was
T9583	4277-4281	IN	denotes	than
T9584	4282-4285	DT	denotes	the
T9585	4302-4312	NN	denotes	production
T9586	4286-4295	VBN	denotes	increased
T9587	4296-4301	NN	denotes	urine
T9588	4313-4316	CC	denotes	and
T9589	4317-4322	NN	denotes	water
T9590	4323-4329	NN	denotes	intake
T9591	4329-4331	,	denotes	,
T9592	4331-4336	EX	denotes	there
T9593	4341-4343	DT	denotes	no
T9594	4350-4359	NN	denotes	phenotype
T9595	4344-4349	JJ	denotes	overt
T9596	4360-4362	IN	denotes	in
T9597	4363-4369	NN	denotes	mutant
T9598	4370-4374	NNS	denotes	mice
T9599	4374-4376	,	denotes	,
T9600	4376-4380	IN	denotes	save
T9601	4381-4390	JJ	denotes	distended
T9602	4391-4398	NNS	denotes	kidneys
T9603	4398-4400	,	denotes	,
T9604	4400-4405	WDT	denotes	which
T9605	4406-4414	VBD	denotes	appeared
T9606	4415-4423	RB	denotes	variably
T9607	4424-4426	IN	denotes	in
T9608	4427-4432	JJ	denotes	adult
T9609	4433-4440	NNS	denotes	animals
T9610	4441-4442	-LRB-	denotes	(
T9611	4442-4448	NN	denotes	Figure
T9612	4449-4451	CD	denotes	2A
T9613	4451-4452	-RRB-	denotes	)
T9614	4452-4453	.	denotes	.
T9615	4453-4533	sentence	denotes	Although not specifically measured, mutant mice seem to have a normal lifespan.
T9616	4454-4462	IN	denotes	Although
T9617	4480-4488	VBN	denotes	measured
T9618	4463-4466	RB	denotes	not
T9619	4467-4479	RB	denotes	specifically
T9620	4502-4506	VBP	denotes	seem
T9621	4488-4490	,	denotes	,
T9622	4490-4496	NN	denotes	mutant
T9623	4497-4501	NNS	denotes	mice
T9624	4507-4509	TO	denotes	to
T9625	4510-4514	VB	denotes	have
T9626	4515-4516	DT	denotes	a
T9627	4524-4532	NN	denotes	lifespan
T9628	4517-4523	JJ	denotes	normal
T9629	4532-4533	.	denotes	.
T9630	4533-4617	sentence	denotes	The one animal that was followed lived to 18 mo, typical for animals in our colony.
T9631	4534-4537	DT	denotes	The
T9632	4542-4548	NN	denotes	animal
T9633	4538-4541	CD	denotes	one
T9634	4567-4572	VBN	denotes	lived
T9635	4549-4553	WDT	denotes	that
T9636	4558-4566	VBN	denotes	followed
T9637	4554-4557	VBD	denotes	was
T9638	4573-4575	IN	denotes	to
T9639	4576-4578	CD	denotes	18
T9640	4579-4581	NNS	denotes	mo
T9641	4581-4583	,	denotes	,
T9642	4583-4590	JJ	denotes	typical
T9643	4591-4594	IN	denotes	for
T9644	4595-4602	NNS	denotes	animals
T9645	4603-4605	IN	denotes	in
T9646	4606-4609	PRP$	denotes	our
T9647	4610-4616	NN	denotes	colony
T9648	4616-4617	.	denotes	.
T9649	4617-5615	sentence	denotes	Figure 2 Anatomy and Histology of Mouse Kidneys (A) Gross anatomy of an affected mouse (8-mo-old male). This shows the enlargement and cystic dilatation of the renal pelvis. There is thinning of the overlying renal parenchyma imparting a translucent appearance to portions of the kidney and collecting system. The bladder is also dilated. (B) Left kidney from mutant mouse (right) shown in (A) compared to a kidney from an age-sex matched unaffected littermate (left). (C) Hematoxylin and eosin stained section of ureter from a mutant mouse, showing normal histology despite bloating of the kidney. (D) Hematoxylin and eosin stained histologic section of a kidney from a 4-wk-old female mutant mouse. The mutant kidney shows marked dilatation of the renal pelvis with blunting of the papilla. There is preservation of the cortex and medulla. Aqp2F204V/F204V mice suffer from severe hydronephrosis (Figure 2A and 2B), presumably as a consequence of an inability to cope with the extreme polyuria.
T9650	5462-5471	NN	denotes	Aqp2F204V
T9651	5472-5477	NN	denotes	F204V
T9652	5471-5472	HYPH	denotes	/
T9653	5478-5482	NNS	denotes	mice
T9654	5483-5489	VBP	denotes	suffer
T9655	5490-5494	IN	denotes	from
T9656	5495-5501	JJ	denotes	severe
T9657	5502-5516	NN	denotes	hydronephrosis
T9658	5517-5518	-LRB-	denotes	(
T9659	5525-5527	CD	denotes	2A
T9660	5518-5524	NN	denotes	Figure
T9661	5528-5531	CC	denotes	and
T9662	5532-5534	CD	denotes	2B
T9663	5534-5535	-RRB-	denotes	)
T9664	5535-5537	,	denotes	,
T9665	5537-5547	RB	denotes	presumably
T9666	5548-5550	IN	denotes	as
T9667	5551-5552	DT	denotes	a
T9668	5553-5564	NN	denotes	consequence
T9669	5565-5567	IN	denotes	of
T9670	5568-5570	DT	denotes	an
T9671	5571-5580	NN	denotes	inability
T9672	5581-5583	TO	denotes	to
T9673	5584-5588	VB	denotes	cope
T9674	5589-5593	IN	denotes	with
T9675	5594-5597	DT	denotes	the
T9676	5606-5614	NN	denotes	polyuria
T9677	5598-5605	JJ	denotes	extreme
T9678	5614-5615	.	denotes	.
T9679	5615-5757	sentence	denotes	We found distended kidneys in all Aqp2F204V/F204V mice; however, the degree of inflation was variable in affected mice and worsened with age.
T9680	5616-5618	PRP	denotes	We
T9681	5619-5624	VBD	denotes	found
T9682	5705-5708	VBD	denotes	was
T9683	5625-5634	JJ	denotes	distended
T9684	5635-5642	NNS	denotes	kidneys
T9685	5643-5645	IN	denotes	in
T9686	5646-5649	DT	denotes	all
T9687	5666-5670	NNS	denotes	mice
T9688	5650-5659	NN	denotes	Aqp2F204V
T9689	5660-5665	NN	denotes	F204V
T9690	5659-5660	HYPH	denotes	/
T9691	5670-5671	:	denotes	;
T9692	5672-5679	RB	denotes	however
T9693	5679-5681	,	denotes	,
T9694	5681-5684	DT	denotes	the
T9695	5685-5691	NN	denotes	degree
T9696	5692-5694	IN	denotes	of
T9697	5695-5704	NN	denotes	inflation
T9698	5709-5717	JJ	denotes	variable
T9699	5718-5720	IN	denotes	in
T9700	5721-5729	VBN	denotes	affected
T9701	5730-5734	NNS	denotes	mice
T9702	5735-5738	CC	denotes	and
T9703	5739-5747	VBD	denotes	worsened
T9704	5748-5752	IN	denotes	with
T9705	5753-5756	NN	denotes	age
T9706	5756-5757	.	denotes	.
T9707	5757-5870	sentence	denotes	Severe hydronephrosis has previously been observed in double Aqp1/Aqp3 knock-out mice [17], and appears at 6 wk.
T9708	5758-5764	JJ	denotes	Severe
T9709	5765-5779	NN	denotes	hydronephrosis
T9710	5800-5808	VBN	denotes	observed
T9711	5780-5783	VBZ	denotes	has
T9712	5784-5794	RB	denotes	previously
T9713	5795-5799	VBN	denotes	been
T9714	5809-5811	IN	denotes	in
T9715	5812-5818	JJ	denotes	double
T9716	5829-5834	VB	denotes	knock
T9717	5819-5823	NN	denotes	Aqp1
T9718	5824-5828	NN	denotes	Aqp3
T9719	5823-5824	HYPH	denotes	/
T9720	5839-5843	NNS	denotes	mice
T9721	5834-5835	HYPH	denotes	-
T9722	5835-5838	RP	denotes	out
T9723	5844-5845	-LRB-	denotes	[
T9724	5845-5847	CD	denotes	17
T9725	5847-5848	-RRB-	denotes	]
T9726	5848-5850	,	denotes	,
T9727	5850-5853	CC	denotes	and
T9728	5854-5861	VBZ	denotes	appears
T9729	5862-5864	IN	denotes	at
T9730	5865-5866	CD	denotes	6
T9731	5867-5869	NNS	denotes	wk
T9732	5869-5870	.	denotes	.
T9733	5870-5925	sentence	denotes	Even at 4 wk, Aqp2F204V/F204V mice had hydronephrosis.
T9734	5871-5875	RB	denotes	Even
T9735	5876-5878	IN	denotes	at
T9736	5906-5909	VBD	denotes	had
T9737	5879-5880	CD	denotes	4
T9738	5881-5883	NNS	denotes	wk
T9739	5883-5885	,	denotes	,
T9740	5885-5894	NN	denotes	Aqp2F204V
T9741	5895-5900	NN	denotes	F204V
T9742	5894-5895	HYPH	denotes	/
T9743	5901-5905	NNS	denotes	mice
T9744	5910-5924	NN	denotes	hydronephrosis
T9745	5924-5925	.	denotes	.
T9746	5925-6078	sentence	denotes	Histologic sections from Aqp2F204V/F204V mice demonstrated marked dilatation of the renal pelvis yet normal morphology of the ureter (Figure 2C and 2D).
T9747	5926-5936	JJ	denotes	Histologic
T9748	5937-5945	NNS	denotes	sections
T9749	5972-5984	VBD	denotes	demonstrated
T9750	5946-5950	IN	denotes	from
T9751	5951-5960	NN	denotes	Aqp2F204V
T9752	5961-5966	NN	denotes	F204V
T9753	5960-5961	HYPH	denotes	/
T9754	5967-5971	NNS	denotes	mice
T9755	5985-5991	JJ	denotes	marked
T9756	5992-6002	NN	denotes	dilatation
T9757	6003-6005	IN	denotes	of
T9758	6006-6009	DT	denotes	the
T9759	6016-6022	NN	denotes	pelvis
T9760	6010-6015	JJ	denotes	renal
T9761	6023-6026	CC	denotes	yet
T9762	6027-6033	JJ	denotes	normal
T9763	6034-6044	NN	denotes	morphology
T9764	6045-6047	IN	denotes	of
T9765	6048-6051	DT	denotes	the
T9766	6052-6058	NN	denotes	ureter
T9767	6059-6060	-LRB-	denotes	(
T9768	6067-6069	CD	denotes	2C
T9769	6060-6066	NN	denotes	Figure
T9770	6070-6073	CC	denotes	and
T9771	6074-6076	CD	denotes	2D
T9772	6076-6077	-RRB-	denotes	)
T9773	6077-6078	.	denotes	.
T9774	6078-6155	sentence	denotes	In particular, the muscularis propria was neither hypertrophied nor thinned.
T9775	6079-6081	IN	denotes	In
T9776	6117-6120	VBD	denotes	was
T9777	6082-6092	JJ	denotes	particular
T9778	6092-6094	,	denotes	,
T9779	6094-6097	DT	denotes	the
T9780	6109-6116	NN	denotes	propria
T9781	6098-6108	NN	denotes	muscularis
T9782	6121-6128	CC	denotes	neither
T9783	6129-6142	JJ	denotes	hypertrophied
T9784	6143-6146	CC	denotes	nor
T9785	6147-6154	JJ	denotes	thinned
T9786	6154-6155	.	denotes	.
T9787	6155-6274	sentence	denotes	There was the normal festooned appearance of the urothelium, and this transitional epithelium was of normal thickness.
T9788	6156-6161	EX	denotes	There
T9789	6162-6165	VBD	denotes	was
T9790	6166-6169	DT	denotes	the
T9791	6187-6197	NN	denotes	appearance
T9792	6170-6176	JJ	denotes	normal
T9793	6177-6186	VBN	denotes	festooned
T9794	6198-6200	IN	denotes	of
T9795	6201-6204	DT	denotes	the
T9796	6205-6215	NN	denotes	urothelium
T9797	6215-6217	,	denotes	,
T9798	6217-6220	CC	denotes	and
T9799	6221-6225	DT	denotes	this
T9800	6239-6249	NN	denotes	epithelium
T9801	6226-6238	JJ	denotes	transitional
T9802	6250-6253	VBD	denotes	was
T9803	6254-6256	IN	denotes	of
T9804	6257-6263	JJ	denotes	normal
T9805	6264-6273	NN	denotes	thickness
T9806	6273-6274	.	denotes	.
T9807	6274-6355	sentence	denotes	There was thinning of the kidney as measured from renal capsule to renal pelvis.
T9808	6275-6280	EX	denotes	There
T9809	6281-6284	VBD	denotes	was
T9810	6285-6293	NN	denotes	thinning
T9811	6294-6296	IN	denotes	of
T9812	6297-6300	DT	denotes	the
T9813	6301-6307	NN	denotes	kidney
T9814	6308-6310	IN	denotes	as
T9815	6311-6319	VBN	denotes	measured
T9816	6320-6324	IN	denotes	from
T9817	6325-6330	JJ	denotes	renal
T9818	6331-6338	NN	denotes	capsule
T9819	6339-6341	IN	denotes	to
T9820	6342-6347	JJ	denotes	renal
T9821	6348-6354	NN	denotes	pelvis
T9822	6354-6355	.	denotes	.
T9823	6355-6465	sentence	denotes	However, the morphologic features of the glomeruli and proximal/distal tubules were unremarkable (Figure 2D).
T9824	6356-6363	RB	denotes	However
T9825	6435-6439	VBD	denotes	were
T9826	6363-6365	,	denotes	,
T9827	6365-6368	DT	denotes	the
T9828	6381-6389	NNS	denotes	features
T9829	6369-6380	JJ	denotes	morphologic
T9830	6390-6392	IN	denotes	of
T9831	6393-6396	DT	denotes	the
T9832	6397-6406	NNS	denotes	glomeruli
T9833	6407-6410	CC	denotes	and
T9834	6411-6419	JJ	denotes	proximal
T9835	6420-6426	JJ	denotes	distal
T9836	6419-6420	HYPH	denotes	/
T9837	6427-6434	NNS	denotes	tubules
T9838	6440-6452	JJ	denotes	unremarkable
T9839	6453-6454	-LRB-	denotes	(
T9840	6454-6460	NN	denotes	Figure
T9841	6461-6463	CD	denotes	2D
T9842	6463-6464	-RRB-	denotes	)
T9843	6464-6465	.	denotes	.
T9844	6465-6614	sentence	denotes	As shown previously [18,22], immunoblotting revealed three different forms of AQP2, due to different degrees and forms of glycosylation (Figure 3A).
T9845	6466-6468	IN	denotes	As
T9846	6469-6474	VBN	denotes	shown
T9847	6510-6518	VBD	denotes	revealed
T9848	6475-6485	RB	denotes	previously
T9849	6486-6487	-LRB-	denotes	[
T9850	6490-6492	CD	denotes	22
T9851	6487-6489	CD	denotes	18
T9852	6489-6490	,	denotes	,
T9853	6492-6493	-RRB-	denotes	]
T9854	6493-6495	,	denotes	,
T9855	6495-6509	NN	denotes	immunoblotting
T9856	6519-6524	CD	denotes	three
T9857	6535-6540	NNS	denotes	forms
T9858	6525-6534	JJ	denotes	different
T9859	6541-6543	IN	denotes	of
T9860	6544-6548	NN	denotes	AQP2
T9861	6548-6550	,	denotes	,
T9862	6550-6553	IN	denotes	due
T9863	6554-6556	IN	denotes	to
T9864	6557-6566	JJ	denotes	different
T9865	6567-6574	NNS	denotes	degrees
T9866	6575-6578	CC	denotes	and
T9867	6579-6584	NNS	denotes	forms
T9868	6585-6587	IN	denotes	of
T9869	6588-6601	NN	denotes	glycosylation
T9870	6602-6603	-LRB-	denotes	(
T9871	6603-6609	NN	denotes	Figure
T9872	6610-6612	CD	denotes	3A
T9873	6612-6613	-RRB-	denotes	)
T9874	6613-6614	.	denotes	.
T9875	6614-6778	sentence	denotes	Previous reports have demonstrated that nonglycosylated protein appears as a 29 kDa band, while complex glycosylated protein runs as a smear between 35 and 45 kDa.
T9876	6615-6623	JJ	denotes	Previous
T9877	6624-6631	NNS	denotes	reports
T9878	6637-6649	VBN	denotes	demonstrated
T9879	6632-6636	VBP	denotes	have
T9880	6650-6654	IN	denotes	that
T9881	6679-6686	VBZ	denotes	appears
T9882	6655-6670	JJ	denotes	nonglycosylated
T9883	6671-6678	NN	denotes	protein
T9884	6687-6689	IN	denotes	as
T9885	6690-6691	DT	denotes	a
T9886	6699-6703	NN	denotes	band
T9887	6692-6694	CD	denotes	29
T9888	6695-6698	NN	denotes	kDa
T9889	6703-6705	,	denotes	,
T9890	6705-6710	IN	denotes	while
T9891	6740-6744	VBZ	denotes	runs
T9892	6711-6718	JJ	denotes	complex
T9893	6732-6739	NN	denotes	protein
T9894	6719-6731	VBN	denotes	glycosylated
T9895	6745-6747	IN	denotes	as
T9896	6748-6749	DT	denotes	a
T9897	6750-6755	NN	denotes	smear
T9898	6756-6763	IN	denotes	between
T9899	6764-6766	CD	denotes	35
T9900	6774-6777	NN	denotes	kDa
T9901	6767-6770	CC	denotes	and
T9902	6771-6773	CD	denotes	45
T9903	6777-6778	.	denotes	.
T9904	6778-6930	sentence	denotes	A short-lived intermediate form of 31 kDa representing core, high-mannose glycosylation of AQP2 is apparent from pulse-chase labeling experiments [22].
T9905	6779-6780	DT	denotes	A
T9906	6806-6810	NN	denotes	form
T9907	6781-6786	JJ	denotes	short
T9908	6787-6792	JJ	denotes	lived
T9909	6786-6787	HYPH	denotes	-
T9910	6793-6805	JJ	denotes	intermediate
T9911	6875-6877	VBZ	denotes	is
T9912	6811-6813	IN	denotes	of
T9913	6814-6816	CD	denotes	31
T9914	6817-6820	NN	denotes	kDa
T9915	6821-6833	VBG	denotes	representing
T9916	6834-6838	NN	denotes	core
T9917	6853-6866	NN	denotes	glycosylation
T9918	6838-6840	,	denotes	,
T9919	6840-6844	JJ	denotes	high
T9920	6845-6852	NN	denotes	mannose
T9921	6844-6845	HYPH	denotes	-
T9922	6867-6869	IN	denotes	of
T9923	6870-6874	NN	denotes	AQP2
T9924	6878-6886	JJ	denotes	apparent
T9925	6887-6891	IN	denotes	from
T9926	6892-6897	NN	denotes	pulse
T9927	6898-6903	NN	denotes	chase
T9928	6897-6898	HYPH	denotes	-
T9929	6904-6912	NN	denotes	labeling
T9930	6913-6924	NNS	denotes	experiments
T9931	6925-6926	-LRB-	denotes	[
T9932	6926-6928	CD	denotes	22
T9933	6928-6929	-RRB-	denotes	]
T9934	6929-6930	.	denotes	.
T9935	6930-7177	sentence	denotes	Compared to that from the kidneys of wild-type animals, AQP2 from mutant animals was reduced in both the high molecular weight, diffuse form and the lowest molecular weight form, but enriched in the intermediate molecular weight form (Figure 3A).
T9936	6931-6939	VBN	denotes	Compared
T9937	7016-7023	VBN	denotes	reduced
T9938	6940-6942	IN	denotes	to
T9939	6943-6947	DT	denotes	that
T9940	6948-6952	IN	denotes	from
T9941	6953-6956	DT	denotes	the
T9942	6957-6964	NNS	denotes	kidneys
T9943	6965-6967	IN	denotes	of
T9944	6968-6972	JJ	denotes	wild
T9945	6973-6977	NN	denotes	type
T9946	6972-6973	HYPH	denotes	-
T9947	6978-6985	NNS	denotes	animals
T9948	6985-6987	,	denotes	,
T9949	6987-6991	NN	denotes	AQP2
T9950	6992-6996	IN	denotes	from
T9951	6997-7003	NN	denotes	mutant
T9952	7004-7011	NNS	denotes	animals
T9953	7012-7015	VBD	denotes	was
T9954	7024-7026	IN	denotes	in
T9955	7027-7031	CC	denotes	both
T9956	7067-7071	NN	denotes	form
T9957	7032-7035	DT	denotes	the
T9958	7036-7040	JJ	denotes	high
T9959	7051-7057	NN	denotes	weight
T9960	7041-7050	JJ	denotes	molecular
T9961	7057-7059	,	denotes	,
T9962	7059-7066	JJ	denotes	diffuse
T9963	7072-7075	CC	denotes	and
T9964	7076-7079	DT	denotes	the
T9965	7104-7108	NN	denotes	form
T9966	7080-7086	JJS	denotes	lowest
T9967	7097-7103	NN	denotes	weight
T9968	7087-7096	JJ	denotes	molecular
T9969	7108-7110	,	denotes	,
T9970	7110-7113	CC	denotes	but
T9971	7114-7122	VBN	denotes	enriched
T9972	7123-7125	IN	denotes	in
T9973	7126-7129	DT	denotes	the
T9974	7160-7164	NN	denotes	form
T9975	7130-7142	JJ	denotes	intermediate
T9976	7153-7159	NN	denotes	weight
T9977	7143-7152	JJ	denotes	molecular
T9978	7165-7166	-LRB-	denotes	(
T9979	7166-7172	NN	denotes	Figure
T9980	7173-7175	CD	denotes	3A
T9981	7175-7176	-RRB-	denotes	)
T9982	7176-7177	.	denotes	.
T9983	7177-7246	sentence	denotes	Heterozygous animals showed intermediate amounts of all three forms.
T9984	7178-7190	JJ	denotes	Heterozygous
T9985	7191-7198	NNS	denotes	animals
T9986	7199-7205	VBD	denotes	showed
T9987	7206-7218	JJ	denotes	intermediate
T9988	7219-7226	NNS	denotes	amounts
T9989	7227-7229	IN	denotes	of
T9990	7230-7233	DT	denotes	all
T9991	7240-7245	NNS	denotes	forms
T9992	7234-7239	CD	denotes	three
T9993	7245-7246	.	denotes	.
T9994	7246-7415	sentence	denotes	The nature of these glycosylated forms was revealed by digestion with endoglycosidase H, which specifically cleaves mannose-rich carbohydrate from the protein backbone.
T9995	7247-7250	DT	denotes	The
T9996	7251-7257	NN	denotes	nature
T9997	7290-7298	VBN	denotes	revealed
T9998	7258-7260	IN	denotes	of
T9999	7261-7266	DT	denotes	these
T10000	7280-7285	NNS	denotes	forms
T10001	7267-7279	VBN	denotes	glycosylated
T10002	7286-7289	VBD	denotes	was
T10003	7299-7301	IN	denotes	by
T10004	7302-7311	NN	denotes	digestion
T10005	7312-7316	IN	denotes	with
T10006	7317-7332	NN	denotes	endoglycosidase
T10007	7333-7334	NN	denotes	H
T10008	7334-7336	,	denotes	,
T10009	7336-7341	WDT	denotes	which
T10010	7355-7362	VBZ	denotes	cleaves
T10011	7342-7354	RB	denotes	specifically
T10012	7363-7370	NN	denotes	mannose
T10013	7371-7375	JJ	denotes	rich
T10014	7370-7371	HYPH	denotes	-
T10015	7376-7388	NN	denotes	carbohydrate
T10016	7389-7393	IN	denotes	from
T10017	7394-7397	DT	denotes	the
T10018	7406-7414	NN	denotes	backbone
T10019	7398-7405	NN	denotes	protein
T10020	7414-7415	.	denotes	.
T10021	7415-7578	sentence	denotes	Treatment of endogenous AQP2 from kidneys of wild-type, heterozygous, and mutant animals specifically affected the intermediate molecular weight form (Figure 3B).
T10022	7416-7425	NN	denotes	Treatment
T10023	7518-7526	VBD	denotes	affected
T10024	7426-7428	IN	denotes	of
T10025	7429-7439	JJ	denotes	endogenous
T10026	7440-7444	NN	denotes	AQP2
T10027	7445-7449	IN	denotes	from
T10028	7450-7457	NNS	denotes	kidneys
T10029	7458-7460	IN	denotes	of
T10030	7461-7465	JJ	denotes	wild
T10031	7466-7470	NN	denotes	type
T10032	7465-7466	HYPH	denotes	-
T10033	7497-7504	NNS	denotes	animals
T10034	7470-7472	,	denotes	,
T10035	7472-7484	JJ	denotes	heterozygous
T10036	7484-7486	,	denotes	,
T10037	7486-7489	CC	denotes	and
T10038	7490-7496	JJ	denotes	mutant
T10039	7505-7517	RB	denotes	specifically
T10040	7527-7530	DT	denotes	the
T10041	7561-7565	NN	denotes	form
T10042	7531-7543	JJ	denotes	intermediate
T10043	7554-7560	NN	denotes	weight
T10044	7544-7553	JJ	denotes	molecular
T10045	7566-7567	-LRB-	denotes	(
T10046	7567-7573	NN	denotes	Figure
T10047	7574-7576	CD	denotes	3B
T10048	7576-7577	-RRB-	denotes	)
T10049	7577-7578	.	denotes	.
T10050	7578-7787	sentence	denotes	The presence of some mature glycosylated proteins (35–45 kDa) in Aqp2F204V/F204V mice presumably permits their survival compared to Aqp2T126M/T126M mice, and is consistent with a diminished response to dDAVP.
T10051	7579-7582	DT	denotes	The
T10052	7583-7591	NN	denotes	presence
T10053	7676-7683	VBZ	denotes	permits
T10054	7592-7594	IN	denotes	of
T10055	7595-7599	DT	denotes	some
T10056	7620-7628	NN	denotes	proteins
T10057	7600-7606	JJ	denotes	mature
T10058	7607-7619	VBN	denotes	glycosylated
T10059	7629-7630	-LRB-	denotes	(
T10060	7636-7639	NN	denotes	kDa
T10061	7630-7632	CD	denotes	35
T10062	7633-7635	CD	denotes	45
T10063	7632-7633	SYM	denotes	–
T10064	7639-7640	-RRB-	denotes	)
T10065	7641-7643	IN	denotes	in
T10066	7644-7653	NN	denotes	Aqp2F204V
T10067	7654-7659	NN	denotes	F204V
T10068	7653-7654	HYPH	denotes	/
T10069	7660-7664	NNS	denotes	mice
T10070	7665-7675	RB	denotes	presumably
T10071	7684-7689	PRP$	denotes	their
T10072	7690-7698	NN	denotes	survival
T10073	7699-7707	VBN	denotes	compared
T10074	7708-7710	IN	denotes	to
T10075	7711-7720	NN	denotes	Aqp2T126M
T10076	7721-7726	NN	denotes	T126M
T10077	7720-7721	HYPH	denotes	/
T10078	7727-7731	NNS	denotes	mice
T10079	7731-7733	,	denotes	,
T10080	7733-7736	CC	denotes	and
T10081	7737-7739	VBZ	denotes	is
T10082	7740-7750	JJ	denotes	consistent
T10083	7751-7755	IN	denotes	with
T10084	7756-7757	DT	denotes	a
T10085	7769-7777	NN	denotes	response
T10086	7758-7768	JJ	denotes	diminished
T10087	7778-7780	IN	denotes	to
T10088	7781-7786	NN	denotes	dDAVP
T10089	7786-7787	.	denotes	.
T10090	7787-8441	sentence	denotes	Figure 3 Immunoblot Analyses of AQP2 from Mouse Kidneys (A) Western blot analyses of total kidney membranes from littermate mice. An intermediate form of AQP2 at 31 kDa was identified in kidney membranes from a mutant mouse (Mut) and partially in a heterozygous mouse (Het). (B) Total kidney membranes were subjected to endoglycosidase H treatment (Endo H) prior to Western blotting. High-mannose (h.m.) glycosylated proteins that have not exited the ER are sensitive to endoglycosidase H digestion. In humans, recessive alleles of Aqp2 are postulated to cause NDI because they do not properly translocate to the apical cell surface in response to AVP.
T10091	8289-8291	IN	denotes	In
T10092	8330-8340	VBN	denotes	postulated
T10093	8292-8298	NNS	denotes	humans
T10094	8298-8300	,	denotes	,
T10095	8300-8309	JJ	denotes	recessive
T10096	8310-8317	NNS	denotes	alleles
T10097	8318-8320	IN	denotes	of
T10098	8321-8325	NN	denotes	Aqp2
T10099	8326-8329	VBP	denotes	are
T10100	8341-8343	TO	denotes	to
T10101	8344-8349	VB	denotes	cause
T10102	8350-8353	NN	denotes	NDI
T10103	8354-8361	IN	denotes	because
T10104	8383-8394	VB	denotes	translocate
T10105	8362-8366	PRP	denotes	they
T10106	8367-8369	VBP	denotes	do
T10107	8370-8373	RB	denotes	not
T10108	8374-8382	RB	denotes	properly
T10109	8395-8397	IN	denotes	to
T10110	8398-8401	DT	denotes	the
T10111	8414-8421	NN	denotes	surface
T10112	8402-8408	JJ	denotes	apical
T10113	8409-8413	NN	denotes	cell
T10114	8422-8424	IN	denotes	in
T10115	8425-8433	NN	denotes	response
T10116	8434-8436	IN	denotes	to
T10117	8437-8440	NN	denotes	AVP
T10118	8440-8441	.	denotes	.
T10119	8441-8599	sentence	denotes	This postulate comes solely from in vitro studies in which mutant Aqp2 cDNAs corresponding to human disease mutations are transfected into kidney cell lines.
T10120	8442-8446	DT	denotes	This
T10121	8447-8456	NN	denotes	postulate
T10122	8457-8462	VBZ	denotes	comes
T10123	8463-8469	RB	denotes	solely
T10124	8470-8474	IN	denotes	from
T10125	8475-8477	FW	denotes	in
T10126	8478-8483	FW	denotes	vitro
T10127	8484-8491	NNS	denotes	studies
T10128	8492-8494	IN	denotes	in
T10129	8564-8575	VBN	denotes	transfected
T10130	8495-8500	WDT	denotes	which
T10131	8501-8507	NN	denotes	mutant
T10132	8513-8518	NNS	denotes	cDNAs
T10133	8508-8512	NN	denotes	Aqp2
T10134	8519-8532	VBG	denotes	corresponding
T10135	8533-8535	IN	denotes	to
T10136	8536-8541	JJ	denotes	human
T10137	8550-8559	NNS	denotes	mutations
T10138	8542-8549	NN	denotes	disease
T10139	8560-8563	VBP	denotes	are
T10140	8576-8580	IN	denotes	into
T10141	8581-8587	NN	denotes	kidney
T10142	8593-8598	NNS	denotes	lines
T10143	8588-8592	NN	denotes	cell
T10144	8598-8599	.	denotes	.
T10145	8599-8718	sentence	denotes	In general, such recessive alleles, when visualized immunocytochemically, fail to localize to AVP-responsive vesicles.
T10146	8600-8602	IN	denotes	In
T10147	8674-8678	VBP	denotes	fail
T10148	8603-8610	JJ	denotes	general
T10149	8610-8612	,	denotes	,
T10150	8612-8616	JJ	denotes	such
T10151	8627-8634	NNS	denotes	alleles
T10152	8617-8626	JJ	denotes	recessive
T10153	8634-8636	,	denotes	,
T10154	8636-8640	WRB	denotes	when
T10155	8641-8651	VBN	denotes	visualized
T10156	8652-8672	RB	denotes	immunocytochemically
T10157	8672-8674	,	denotes	,
T10158	8679-8681	TO	denotes	to
T10159	8682-8690	VB	denotes	localize
T10160	8691-8693	IN	denotes	to
T10161	8694-8697	NN	denotes	AVP
T10162	8698-8708	JJ	denotes	responsive
T10163	8697-8698	HYPH	denotes	-
T10164	8709-8717	NNS	denotes	vesicles
T10165	8717-8718	.	denotes	.
T10166	8718-8778	sentence	denotes	Rather, they get trapped in the endoplasmic reticulum (ER).
T10167	8719-8725	RB	denotes	Rather
T10168	8736-8743	VBN	denotes	trapped
T10169	8725-8727	,	denotes	,
T10170	8727-8731	PRP	denotes	they
T10171	8732-8735	VBP	denotes	get
T10172	8744-8746	IN	denotes	in
T10173	8747-8750	DT	denotes	the
T10174	8763-8772	NN	denotes	reticulum
T10175	8751-8762	JJ	denotes	endoplasmic
T10176	8773-8774	-LRB-	denotes	(
T10177	8774-8776	NN	denotes	ER
T10178	8776-8777	-RRB-	denotes	)
T10179	8777-8778	.	denotes	.
T10180	8778-8875	sentence	denotes	Our mouse model of NDI affords the first opportunity to test this hypothesis in a mature animal.
T10181	8779-8782	PRP$	denotes	Our
T10182	8789-8794	NN	denotes	model
T10183	8783-8788	NN	denotes	mouse
T10184	8802-8809	VBZ	denotes	affords
T10185	8795-8797	IN	denotes	of
T10186	8798-8801	NN	denotes	NDI
T10187	8810-8813	DT	denotes	the
T10188	8820-8831	NN	denotes	opportunity
T10189	8814-8819	JJ	denotes	first
T10190	8832-8834	TO	denotes	to
T10191	8835-8839	VB	denotes	test
T10192	8840-8844	DT	denotes	this
T10193	8845-8855	NN	denotes	hypothesis
T10194	8856-8858	IN	denotes	in
T10195	8859-8860	DT	denotes	a
T10196	8868-8874	NN	denotes	animal
T10197	8861-8867	JJ	denotes	mature
T10198	8874-8875	.	denotes	.
T10199	8875-9045	sentence	denotes	As shown in Figure 4A (top row of photomicrographs), AQP2 (stained red) normally localized to the subapical region of collecting duct cells in kidneys of wild-type mice.
T10200	8876-8878	IN	denotes	As
T10201	8879-8884	VBN	denotes	shown
T10202	8957-8966	VBD	denotes	localized
T10203	8885-8887	IN	denotes	in
T10204	8888-8894	NN	denotes	Figure
T10205	8895-8897	CD	denotes	4A
T10206	8898-8899	-LRB-	denotes	(
T10207	8903-8906	NN	denotes	row
T10208	8899-8902	JJ	denotes	top
T10209	8907-8909	IN	denotes	of
T10210	8910-8926	NNS	denotes	photomicrographs
T10211	8926-8927	-RRB-	denotes	)
T10212	8927-8929	,	denotes	,
T10213	8929-8933	NN	denotes	AQP2
T10214	8934-8935	-LRB-	denotes	(
T10215	8935-8942	VBN	denotes	stained
T10216	8943-8946	JJ	denotes	red
T10217	8946-8947	-RRB-	denotes	)
T10218	8948-8956	RB	denotes	normally
T10219	8967-8969	IN	denotes	to
T10220	8970-8973	DT	denotes	the
T10221	8984-8990	NN	denotes	region
T10222	8974-8983	JJ	denotes	subapical
T10223	8991-8993	IN	denotes	of
T10224	8994-9004	VBG	denotes	collecting
T10225	9005-9009	NN	denotes	duct
T10226	9010-9015	NNS	denotes	cells
T10227	9016-9018	IN	denotes	in
T10228	9019-9026	NNS	denotes	kidneys
T10229	9027-9029	IN	denotes	of
T10230	9030-9034	JJ	denotes	wild
T10231	9035-9039	NN	denotes	type
T10232	9034-9035	HYPH	denotes	-
T10233	9040-9044	NNS	denotes	mice
T10234	9044-9045	.	denotes	.
T10235	9045-9145	sentence	denotes	Upon stimulation with dDAVP, AQP2 translocated to or near the cell surface (Figure 4A, second row).
T10236	9046-9050	IN	denotes	Upon
T10237	9080-9092	VBD	denotes	translocated
T10238	9051-9062	NN	denotes	stimulation
T10239	9063-9067	IN	denotes	with
T10240	9068-9073	NN	denotes	dDAVP
T10241	9073-9075	,	denotes	,
T10242	9075-9079	NN	denotes	AQP2
T10243	9093-9095	IN	denotes	to
T10244	9096-9098	CC	denotes	or
T10245	9099-9103	IN	denotes	near
T10246	9104-9107	DT	denotes	the
T10247	9113-9120	NN	denotes	surface
T10248	9108-9112	NN	denotes	cell
T10249	9121-9122	-LRB-	denotes	(
T10250	9140-9143	NN	denotes	row
T10251	9122-9128	NN	denotes	Figure
T10252	9129-9131	CD	denotes	4A
T10253	9131-9133	,	denotes	,
T10254	9133-9139	JJ	denotes	second
T10255	9143-9144	-RRB-	denotes	)
T10256	9144-9145	.	denotes	.
T10257	9145-9361	sentence	denotes	In kidneys taken from mutant animals, however, AQP2 was distributed randomly throughout the cell in the basal state (Figure 4A, third row), while AQP3 (green) appropriately localized to the basolateral surface [23].
T10258	9146-9148	IN	denotes	In
T10259	9202-9213	VBN	denotes	distributed
T10260	9149-9156	NNS	denotes	kidneys
T10261	9157-9162	VBN	denotes	taken
T10262	9163-9167	IN	denotes	from
T10263	9168-9174	NN	denotes	mutant
T10264	9175-9182	NNS	denotes	animals
T10265	9182-9184	,	denotes	,
T10266	9184-9191	RB	denotes	however
T10267	9191-9193	,	denotes	,
T10268	9193-9197	NN	denotes	AQP2
T10269	9198-9201	VBD	denotes	was
T10270	9214-9222	RB	denotes	randomly
T10271	9223-9233	IN	denotes	throughout
T10272	9234-9237	DT	denotes	the
T10273	9238-9242	NN	denotes	cell
T10274	9243-9245	IN	denotes	in
T10275	9246-9249	DT	denotes	the
T10276	9256-9261	NN	denotes	state
T10277	9250-9255	JJ	denotes	basal
T10278	9262-9263	-LRB-	denotes	(
T10279	9280-9283	NN	denotes	row
T10280	9263-9269	NN	denotes	Figure
T10281	9270-9272	CD	denotes	4A
T10282	9272-9274	,	denotes	,
T10283	9274-9279	JJ	denotes	third
T10284	9283-9284	-RRB-	denotes	)
T10285	9284-9286	,	denotes	,
T10286	9286-9291	IN	denotes	while
T10287	9319-9328	VBD	denotes	localized
T10288	9292-9296	NN	denotes	AQP3
T10289	9297-9298	-LRB-	denotes	(
T10290	9298-9303	NN	denotes	green
T10291	9303-9304	-RRB-	denotes	)
T10292	9305-9318	RB	denotes	appropriately
T10293	9329-9331	IN	denotes	to
T10294	9332-9335	DT	denotes	the
T10295	9348-9355	NN	denotes	surface
T10296	9336-9347	JJ	denotes	basolateral
T10297	9356-9357	-LRB-	denotes	[
T10298	9357-9359	CD	denotes	23
T10299	9359-9360	-RRB-	denotes	]
T10300	9360-9361	.	denotes	.
T10301	9361-9476	sentence	denotes	Furthermore, upon dDAVP stimulation, AQP2-F204V failed to translocate to the cell surface (Figure 4A, bottom row).
T10302	9362-9373	RB	denotes	Furthermore
T10303	9410-9416	VBD	denotes	failed
T10304	9373-9375	,	denotes	,
T10305	9375-9379	IN	denotes	upon
T10306	9380-9385	NN	denotes	dDAVP
T10307	9386-9397	NN	denotes	stimulation
T10308	9397-9399	,	denotes	,
T10309	9399-9403	NN	denotes	AQP2
T10310	9404-9409	NN	denotes	F204V
T10311	9403-9404	HYPH	denotes	-
T10312	9417-9419	TO	denotes	to
T10313	9420-9431	VB	denotes	translocate
T10314	9432-9434	IN	denotes	to
T10315	9435-9438	DT	denotes	the
T10316	9444-9451	NN	denotes	surface
T10317	9439-9443	NN	denotes	cell
T10318	9452-9453	-LRB-	denotes	(
T10319	9471-9474	NN	denotes	row
T10320	9453-9459	NN	denotes	Figure
T10321	9460-9462	CD	denotes	4A
T10322	9462-9464	,	denotes	,
T10323	9464-9470	JJ	denotes	bottom
T10324	9474-9475	-RRB-	denotes	)
T10325	9475-9476	.	denotes	.
T10326	9476-9662	sentence	denotes	To confirm these findings, the staining was repeated in kidneys taken from two further mice for each class, wild-type or mutant, with or without dDAVP treatment, with identical results.
T10327	9477-9479	TO	denotes	To
T10328	9480-9487	VB	denotes	confirm
T10329	9521-9529	VBN	denotes	repeated
T10330	9488-9493	DT	denotes	these
T10331	9494-9502	NNS	denotes	findings
T10332	9502-9504	,	denotes	,
T10333	9504-9507	DT	denotes	the
T10334	9508-9516	NN	denotes	staining
T10335	9517-9520	VBD	denotes	was
T10336	9530-9532	IN	denotes	in
T10337	9533-9540	NNS	denotes	kidneys
T10338	9541-9546	VBN	denotes	taken
T10339	9547-9551	IN	denotes	from
T10340	9552-9555	CD	denotes	two
T10341	9564-9568	NNS	denotes	mice
T10342	9556-9563	JJ	denotes	further
T10343	9569-9572	IN	denotes	for
T10344	9573-9577	DT	denotes	each
T10345	9578-9583	NN	denotes	class
T10346	9583-9585	,	denotes	,
T10347	9585-9589	JJ	denotes	wild
T10348	9590-9594	NN	denotes	type
T10349	9589-9590	HYPH	denotes	-
T10350	9595-9597	CC	denotes	or
T10351	9598-9604	NN	denotes	mutant
T10352	9604-9606	,	denotes	,
T10353	9606-9610	IN	denotes	with
T10354	9611-9613	CC	denotes	or
T10355	9614-9621	IN	denotes	without
T10356	9622-9627	NN	denotes	dDAVP
T10357	9628-9637	NN	denotes	treatment
T10358	9637-9639	,	denotes	,
T10359	9639-9643	IN	denotes	with
T10360	9644-9653	JJ	denotes	identical
T10361	9654-9661	NNS	denotes	results
T10362	9661-9662	.	denotes	.
T10363	9662-11442	sentence	denotes	Figure 4 AQP2 Subcellular Localization and Translocation in Mouse Kidney Collecting Ducts and MDCK Cell Lines (A) Immunohistochemistry on collecting ducts in kidney sections from an AQP2-F204V mutant (Mut) mouse and an age-sex matched wild-type (WT) littermate. Mice were injected intraperitoneally with PBS (NT) or dDAVP before sacrificing and fixation of the kidneys. Kidneys sections were immunostained for AQP2 (red) and the basolateral marker AQP3 (green). The images were merged and an area of the cytoplasm was magnified (zoom). Note that mutant AQP2 is not properly localized to the subapical compartment, nor does it respond to dDAVP. (B) MDCK cell lines, stably transfected with constructs encoding mouse WT or AQP2-F204V, were treated with and without 150 μM forskolin for 90 min, after which cells were fixed, permeabilized, and subjected to immunocytochemistry. AQP2 is shown in green, and the basolateral marker Na+/K+-ATPase is shown in red, alongside the nuclear stain DAPI. The z-profile images were reconstructed from multiple z-sections, along the dotted line. Mutant AQP2 fails to localize to the cell surface upon forskolin stimulation. Rather, the perinuclear staining is consistent with an ER localization of mutant AQP2. (C) The MDCK cell line expressing AQP2-F204V was grown on fibronectin-coated coverslips until tight junctions formed, at which point the cells were treated with 150 μM forskolin for 90 min. Cells were fixed, permeabilized, and sequentially immunoblotted for AQP2 (green) and calnexin (red), an ER marker. The merged image shows that AQP2-F204V colocalizes with the endoplasmic reticulum marker. Scale bar refers to 10 μm. To investigate the mechanism of defective translocation of AQP2-F204V, we turned to transfection of MDCK cells.
T10364	11331-11333	TO	denotes	To
T10365	11334-11345	VB	denotes	investigate
T10366	11405-11411	VBD	denotes	turned
T10367	11346-11349	DT	denotes	the
T10368	11350-11359	NN	denotes	mechanism
T10369	11360-11362	IN	denotes	of
T10370	11363-11372	JJ	denotes	defective
T10371	11373-11386	NN	denotes	translocation
T10372	11387-11389	IN	denotes	of
T10373	11390-11394	NN	denotes	AQP2
T10374	11395-11400	NN	denotes	F204V
T10375	11394-11395	HYPH	denotes	-
T10376	11400-11402	,	denotes	,
T10377	11402-11404	PRP	denotes	we
T10378	11412-11414	IN	denotes	to
T10379	11415-11427	NN	denotes	transfection
T10380	11428-11430	IN	denotes	of
T10381	11431-11435	NN	denotes	MDCK
T10382	11436-11441	NNS	denotes	cells
T10383	11441-11442	.	denotes	.
T10384	11442-11525	sentence	denotes	Stable cell lines expressing mouse wild-type AQP2 and AQP2-F204V were established.
T10385	11443-11449	JJ	denotes	Stable
T10386	11455-11460	NNS	denotes	lines
T10387	11450-11454	NN	denotes	cell
T10388	11513-11524	VBN	denotes	established
T10389	11461-11471	VBG	denotes	expressing
T10390	11472-11477	NN	denotes	mouse
T10391	11488-11492	NN	denotes	AQP2
T10392	11478-11482	JJ	denotes	wild
T10393	11483-11487	NN	denotes	type
T10394	11482-11483	HYPH	denotes	-
T10395	11493-11496	CC	denotes	and
T10396	11497-11501	NN	denotes	AQP2
T10397	11502-11507	NN	denotes	F204V
T10398	11501-11502	HYPH	denotes	-
T10399	11508-11512	VBD	denotes	were
T10400	11524-11525	.	denotes	.
T10401	11525-11681	sentence	denotes	Immunoblots of protein extracts from stable cell lines showed that MDCK cells recapitulate the glycosylation defect seen in mutant mice (unpublished data).
T10402	11526-11537	NNS	denotes	Immunoblots
T10403	11581-11587	VBD	denotes	showed
T10404	11538-11540	IN	denotes	of
T10405	11541-11548	NN	denotes	protein
T10406	11549-11557	NNS	denotes	extracts
T10407	11558-11562	IN	denotes	from
T10408	11563-11569	JJ	denotes	stable
T10409	11575-11580	NNS	denotes	lines
T10410	11570-11574	NN	denotes	cell
T10411	11588-11592	IN	denotes	that
T10412	11604-11616	VBP	denotes	recapitulate
T10413	11593-11597	NN	denotes	MDCK
T10414	11598-11603	NNS	denotes	cells
T10415	11617-11620	DT	denotes	the
T10416	11635-11641	NN	denotes	defect
T10417	11621-11634	NN	denotes	glycosylation
T10418	11642-11646	VBN	denotes	seen
T10419	11647-11649	IN	denotes	in
T10420	11650-11656	NN	denotes	mutant
T10421	11657-11661	NNS	denotes	mice
T10422	11662-11663	-LRB-	denotes	(
T10423	11675-11679	NNS	denotes	data
T10424	11663-11674	JJ	denotes	unpublished
T10425	11679-11680	-RRB-	denotes	)
T10426	11680-11681	.	denotes	.
T10427	11681-11747	sentence	denotes	The wild-type protein was again present in three different forms.
T10428	11682-11685	DT	denotes	The
T10429	11696-11703	NN	denotes	protein
T10430	11686-11690	JJ	denotes	wild
T10431	11691-11695	NN	denotes	type
T10432	11690-11691	HYPH	denotes	-
T10433	11704-11707	VBD	denotes	was
T10434	11708-11713	RB	denotes	again
T10435	11714-11721	JJ	denotes	present
T10436	11722-11724	IN	denotes	in
T10437	11725-11730	CD	denotes	three
T10438	11741-11746	NNS	denotes	forms
T10439	11731-11740	JJ	denotes	different
T10440	11746-11747	.	denotes	.
T10441	11747-11857	sentence	denotes	Cells expressing AQP2-F204V lacked the 35–45 kDa form and were enriched in the core-glycosylated 31 kDa form.
T10442	11748-11753	NNS	denotes	Cells
T10443	11776-11782	VBD	denotes	lacked
T10444	11754-11764	VBG	denotes	expressing
T10445	11765-11769	NN	denotes	AQP2
T10446	11770-11775	NN	denotes	F204V
T10447	11769-11770	HYPH	denotes	-
T10448	11783-11786	DT	denotes	the
T10449	11797-11801	NN	denotes	form
T10450	11787-11789	CD	denotes	35
T10451	11790-11792	CD	denotes	45
T10452	11789-11790	SYM	denotes	–
T10453	11793-11796	NN	denotes	kDa
T10454	11802-11805	CC	denotes	and
T10455	11806-11810	VBD	denotes	were
T10456	11811-11819	VBN	denotes	enriched
T10457	11820-11822	IN	denotes	in
T10458	11823-11826	DT	denotes	the
T10459	11852-11856	NN	denotes	form
T10460	11827-11831	NN	denotes	core
T10461	11832-11844	VBN	denotes	glycosylated
T10462	11831-11832	HYPH	denotes	-
T10463	11845-11847	CD	denotes	31
T10464	11848-11851	NN	denotes	kDa
T10465	11856-11857	.	denotes	.
T10466	11857-12095	sentence	denotes	In transfected, unstimulated MDCK cells, wild-type AQP2 (stained green) appeared in a punctate pattern distributed throughout the subapical region (Figure 4B, left column photomicrographs), consistent with vesicular compartmentalization.
T10467	11858-11860	IN	denotes	In
T10468	11930-11938	VBD	denotes	appeared
T10469	11861-11872	VBN	denotes	transfected
T10470	11892-11897	NNS	denotes	cells
T10471	11872-11874	,	denotes	,
T10472	11874-11886	JJ	denotes	unstimulated
T10473	11887-11891	NN	denotes	MDCK
T10474	11897-11899	,	denotes	,
T10475	11899-11903	JJ	denotes	wild
T10476	11904-11908	NN	denotes	type
T10477	11903-11904	HYPH	denotes	-
T10478	11909-11913	NN	denotes	AQP2
T10479	11914-11915	-LRB-	denotes	(
T10480	11915-11922	VBN	denotes	stained
T10481	11923-11928	JJ	denotes	green
T10482	11928-11929	-RRB-	denotes	)
T10483	11939-11941	IN	denotes	in
T10484	11942-11943	DT	denotes	a
T10485	11953-11960	NN	denotes	pattern
T10486	11944-11952	NN	denotes	punctate
T10487	11961-11972	VBN	denotes	distributed
T10488	11973-11983	IN	denotes	throughout
T10489	11984-11987	DT	denotes	the
T10490	11998-12004	NN	denotes	region
T10491	11988-11997	JJ	denotes	subapical
T10492	12005-12006	-LRB-	denotes	(
T10493	12029-12045	NNS	denotes	photomicrographs
T10494	12006-12012	NN	denotes	Figure
T10495	12013-12015	CD	denotes	4B
T10496	12015-12017	,	denotes	,
T10497	12017-12021	JJ	denotes	left
T10498	12022-12028	NN	denotes	column
T10499	12045-12046	-RRB-	denotes	)
T10500	12046-12048	,	denotes	,
T10501	12048-12058	JJ	denotes	consistent
T10502	12059-12063	IN	denotes	with
T10503	12064-12073	JJ	denotes	vesicular
T10504	12074-12094	NN	denotes	compartmentalization
T10505	12094-12095	.	denotes	.
T10506	12095-12200	sentence	denotes	AQP2-F204V, on the other hand, appeared in a punctate but perinuclear pattern (Figure 4B, third column).
T10507	12096-12100	NN	denotes	AQP2
T10508	12101-12106	NN	denotes	F204V
T10509	12100-12101	HYPH	denotes	-
T10510	12127-12135	VBD	denotes	appeared
T10511	12106-12108	,	denotes	,
T10512	12108-12110	IN	denotes	on
T10513	12111-12114	DT	denotes	the
T10514	12121-12125	NN	denotes	hand
T10515	12115-12120	JJ	denotes	other
T10516	12125-12127	,	denotes	,
T10517	12136-12138	IN	denotes	in
T10518	12139-12140	DT	denotes	a
T10519	12166-12173	NN	denotes	pattern
T10520	12141-12149	NN	denotes	punctate
T10521	12154-12165	JJ	denotes	perinuclear
T10522	12150-12153	CC	denotes	but
T10523	12174-12175	-LRB-	denotes	(
T10524	12192-12198	NN	denotes	column
T10525	12175-12181	NN	denotes	Figure
T10526	12182-12184	CD	denotes	4B
T10527	12184-12186	,	denotes	,
T10528	12186-12191	JJ	denotes	third
T10529	12198-12199	-RRB-	denotes	)
T10530	12199-12200	.	denotes	.
T10531	12200-12378	sentence	denotes	Upon stimulation with forskolin, a cAMP-dependent protein kinase activator, wild-type AQP2 translocated to the apical surface of polarized MDCK cells (Figure 4B, second column).
T10532	12201-12205	IN	denotes	Upon
T10533	12292-12304	VBD	denotes	translocated
T10534	12206-12217	NN	denotes	stimulation
T10535	12218-12222	IN	denotes	with
T10536	12223-12232	NN	denotes	forskolin
T10537	12232-12234	,	denotes	,
T10538	12234-12235	DT	denotes	a
T10539	12266-12275	NN	denotes	activator
T10540	12236-12240	NN	denotes	cAMP
T10541	12241-12250	JJ	denotes	dependent
T10542	12240-12241	HYPH	denotes	-
T10543	12251-12258	NN	denotes	protein
T10544	12259-12265	NN	denotes	kinase
T10545	12275-12277	,	denotes	,
T10546	12277-12281	JJ	denotes	wild
T10547	12282-12286	NN	denotes	type
T10548	12281-12282	HYPH	denotes	-
T10549	12287-12291	NN	denotes	AQP2
T10550	12305-12307	IN	denotes	to
T10551	12308-12311	DT	denotes	the
T10552	12319-12326	NN	denotes	surface
T10553	12312-12318	JJ	denotes	apical
T10554	12327-12329	IN	denotes	of
T10555	12330-12339	VBN	denotes	polarized
T10556	12345-12350	NNS	denotes	cells
T10557	12340-12344	NN	denotes	MDCK
T10558	12351-12352	-LRB-	denotes	(
T10559	12370-12376	NN	denotes	column
T10560	12352-12358	NN	denotes	Figure
T10561	12359-12361	CD	denotes	4B
T10562	12361-12363	,	denotes	,
T10563	12363-12369	JJ	denotes	second
T10564	12376-12377	-RRB-	denotes	)
T10565	12377-12378	.	denotes	.
T10566	12378-12551	sentence	denotes	Along the z-axis, the perinuclear distribution of AQP2-F204V was clearly seen, and this distribution is not altered by forskolin (Figure 4B, bottom row, two right columns).
T10567	12379-12384	IN	denotes	Along
T10568	12452-12456	VBN	denotes	seen
T10569	12385-12388	DT	denotes	the
T10570	12391-12395	NN	denotes	axis
T10571	12389-12390	NN	denotes	z
T10572	12390-12391	HYPH	denotes	-
T10573	12395-12397	,	denotes	,
T10574	12397-12400	DT	denotes	the
T10575	12413-12425	NN	denotes	distribution
T10576	12401-12412	JJ	denotes	perinuclear
T10577	12426-12428	IN	denotes	of
T10578	12429-12433	NN	denotes	AQP2
T10579	12434-12439	NN	denotes	F204V
T10580	12433-12434	HYPH	denotes	-
T10581	12440-12443	VBD	denotes	was
T10582	12444-12451	RB	denotes	clearly
T10583	12456-12458	,	denotes	,
T10584	12458-12461	CC	denotes	and
T10585	12462-12466	DT	denotes	this
T10586	12467-12479	NN	denotes	distribution
T10587	12487-12494	VBN	denotes	altered
T10588	12480-12482	VBZ	denotes	is
T10589	12483-12486	RB	denotes	not
T10590	12495-12497	IN	denotes	by
T10591	12498-12507	NN	denotes	forskolin
T10592	12508-12509	-LRB-	denotes	(
T10593	12542-12549	NNS	denotes	columns
T10594	12509-12515	NN	denotes	Figure
T10595	12516-12518	CD	denotes	4B
T10596	12518-12520	,	denotes	,
T10597	12520-12526	JJ	denotes	bottom
T10598	12527-12530	NN	denotes	row
T10599	12530-12532	,	denotes	,
T10600	12532-12535	CD	denotes	two
T10601	12536-12541	JJ	denotes	right
T10602	12549-12550	-RRB-	denotes	)
T10603	12550-12551	.	denotes	.
T10604	12551-12641	sentence	denotes	The perinuclear distribution of AQP2-F204V is consistent with an ER compartmentalization.
T10605	12552-12555	DT	denotes	The
T10606	12568-12580	NN	denotes	distribution
T10607	12556-12567	JJ	denotes	perinuclear
T10608	12595-12597	VBZ	denotes	is
T10609	12581-12583	IN	denotes	of
T10610	12584-12588	NN	denotes	AQP2
T10611	12589-12594	NN	denotes	F204V
T10612	12588-12589	HYPH	denotes	-
T10613	12598-12608	JJ	denotes	consistent
T10614	12609-12613	IN	denotes	with
T10615	12614-12616	DT	denotes	an
T10616	12620-12640	NN	denotes	compartmentalization
T10617	12617-12619	NN	denotes	ER
T10618	12640-12641	.	denotes	.
T10619	12641-12798	sentence	denotes	To test the idea that AQP2-F204V localizes to the ER, we co-stained cells transfected with Aqp2F204V (cDNA) for AQP2 and an ER marker, calnexin (Figure 4C).
T10620	12642-12644	TO	denotes	To
T10621	12645-12649	VB	denotes	test
T10622	12699-12709	VBD	denotes	co-stained
T10623	12650-12653	DT	denotes	the
T10624	12654-12658	NN	denotes	idea
T10625	12659-12663	IN	denotes	that
T10626	12675-12684	VBZ	denotes	localizes
T10627	12664-12668	NN	denotes	AQP2
T10628	12669-12674	NN	denotes	F204V
T10629	12668-12669	HYPH	denotes	-
T10630	12685-12687	IN	denotes	to
T10631	12688-12691	DT	denotes	the
T10632	12692-12694	NN	denotes	ER
T10633	12694-12696	,	denotes	,
T10634	12696-12698	PRP	denotes	we
T10635	12710-12715	NNS	denotes	cells
T10636	12716-12727	VBN	denotes	transfected
T10637	12728-12732	IN	denotes	with
T10638	12733-12742	NN	denotes	Aqp2F204V
T10639	12743-12744	-LRB-	denotes	(
T10640	12744-12748	NN	denotes	cDNA
T10641	12748-12749	-RRB-	denotes	)
T10642	12750-12753	IN	denotes	for
T10643	12754-12758	NN	denotes	AQP2
T10644	12759-12762	CC	denotes	and
T10645	12763-12765	DT	denotes	an
T10646	12769-12775	NN	denotes	marker
T10647	12766-12768	NN	denotes	ER
T10648	12775-12777	,	denotes	,
T10649	12777-12785	NN	denotes	calnexin
T10650	12786-12787	-LRB-	denotes	(
T10651	12787-12793	NN	denotes	Figure
T10652	12794-12796	CD	denotes	4C
T10653	12796-12797	-RRB-	denotes	)
T10654	12797-12798	.	denotes	.
T10655	12798-12941	sentence	denotes	Colocalization of calnexin with AQP2 was investigated directly, and it was found that 80% of all AQP2-F204V protein colocalized with calnexin.
T10656	12799-12813	NN	denotes	Colocalization
T10657	12840-12852	VBN	denotes	investigated
T10658	12814-12816	IN	denotes	of
T10659	12817-12825	NN	denotes	calnexin
T10660	12826-12830	IN	denotes	with
T10661	12831-12835	NN	denotes	AQP2
T10662	12836-12839	VBD	denotes	was
T10663	12853-12861	RB	denotes	directly
T10664	12861-12863	,	denotes	,
T10665	12863-12866	CC	denotes	and
T10666	12867-12869	PRP	denotes	it
T10667	12874-12879	VBN	denotes	found
T10668	12870-12873	VBD	denotes	was
T10669	12880-12884	IN	denotes	that
T10670	12915-12926	VBD	denotes	colocalized
T10671	12885-12887	CD	denotes	80
T10672	12887-12888	NN	denotes	%
T10673	12889-12891	IN	denotes	of
T10674	12892-12895	DT	denotes	all
T10675	12907-12914	NN	denotes	protein
T10676	12896-12900	NN	denotes	AQP2
T10677	12901-12906	NN	denotes	F204V
T10678	12900-12901	HYPH	denotes	-
T10679	12927-12931	IN	denotes	with
T10680	12932-12940	NN	denotes	calnexin
T10681	12940-12941	.	denotes	.
T10682	12941-13067	sentence	denotes	The remaining 20% appeared at the periphery of the ER, representing AQP2-F204V that had potentially progressed beyond the ER.
T10683	12942-12945	DT	denotes	The
T10684	12958-12959	NN	denotes	%
T10685	12946-12955	VBG	denotes	remaining
T10686	12956-12958	CD	denotes	20
T10687	12960-12968	VBD	denotes	appeared
T10688	12969-12971	IN	denotes	at
T10689	12972-12975	DT	denotes	the
T10690	12976-12985	NN	denotes	periphery
T10691	12986-12988	IN	denotes	of
T10692	12989-12992	DT	denotes	the
T10693	12993-12995	NN	denotes	ER
T10694	12995-12997	,	denotes	,
T10695	12997-13009	VBG	denotes	representing
T10696	13010-13014	NN	denotes	AQP2
T10697	13015-13020	NN	denotes	F204V
T10698	13014-13015	HYPH	denotes	-
T10699	13021-13025	WDT	denotes	that
T10700	13042-13052	VBN	denotes	progressed
T10701	13026-13029	VBD	denotes	had
T10702	13030-13041	RB	denotes	potentially
T10703	13053-13059	IN	denotes	beyond
T10704	13060-13063	DT	denotes	the
T10705	13064-13066	NN	denotes	ER
T10706	13066-13067	.	denotes	.
T10707	13067-13204	sentence	denotes	This “ER escape” was consistent with the small proportion of mature, complex glycosylated, AQP2-F204V in mutant kidneys (see Figure 3A).
T10708	13068-13072	DT	denotes	This
T10709	13077-13083	NN	denotes	escape
T10710	13073-13074	``	denotes	“
T10711	13074-13076	NN	denotes	ER
T10712	13085-13088	VBD	denotes	was
T10713	13083-13084	''	denotes	”
T10714	13089-13099	JJ	denotes	consistent
T10715	13100-13104	IN	denotes	with
T10716	13105-13108	DT	denotes	the
T10717	13115-13125	NN	denotes	proportion
T10718	13109-13114	JJ	denotes	small
T10719	13126-13128	IN	denotes	of
T10720	13129-13135	JJ	denotes	mature
T10721	13164-13169	NN	denotes	F204V
T10722	13135-13137	,	denotes	,
T10723	13137-13144	JJ	denotes	complex
T10724	13145-13157	VBN	denotes	glycosylated
T10725	13157-13159	,	denotes	,
T10726	13159-13163	NN	denotes	AQP2
T10727	13163-13164	HYPH	denotes	-
T10728	13170-13172	IN	denotes	in
T10729	13173-13179	NN	denotes	mutant
T10730	13180-13187	NNS	denotes	kidneys
T10731	13188-13189	-LRB-	denotes	(
T10732	13189-13192	VB	denotes	see
T10733	13193-13199	NN	denotes	Figure
T10734	13200-13202	CD	denotes	3A
T10735	13202-13203	-RRB-	denotes	)
T10736	13203-13204	.	denotes	.
T10737	13204-13340	sentence	denotes	Animals heterozygous for the Aqp2F204V mutation were not affected in their urine production or urine osmolality (see Figure 1C and 1D).
T10738	13205-13212	NNS	denotes	Animals
T10739	13262-13270	VBN	denotes	affected
T10740	13213-13225	JJ	denotes	heterozygous
T10741	13226-13229	IN	denotes	for
T10742	13230-13233	DT	denotes	the
T10743	13244-13252	NN	denotes	mutation
T10744	13234-13243	NN	denotes	Aqp2F204V
T10745	13253-13257	VBD	denotes	were
T10746	13258-13261	RB	denotes	not
T10747	13271-13273	IN	denotes	in
T10748	13274-13279	PRP$	denotes	their
T10749	13286-13296	NN	denotes	production
T10750	13280-13285	NN	denotes	urine
T10751	13297-13299	CC	denotes	or
T10752	13300-13305	NN	denotes	urine
T10753	13306-13316	NN	denotes	osmolality
T10754	13317-13318	-LRB-	denotes	(
T10755	13318-13321	VB	denotes	see
T10756	13322-13328	NN	denotes	Figure
T10757	13329-13331	CD	denotes	1C
T10758	13332-13335	CC	denotes	and
T10759	13336-13338	CD	denotes	1D
T10760	13338-13339	-RRB-	denotes	)
T10761	13339-13340	.	denotes	.
T10762	13340-13511	sentence	denotes	It has also been shown that a recessive NDI allele, AQP2-R187C, does not interact with wild-type protein in oocytes [24], nor does it homo-oligomerize in MDCK cells [22].
T10763	13341-13343	PRP	denotes	It
T10764	13358-13363	VBN	denotes	shown
T10765	13344-13347	VBZ	denotes	has
T10766	13348-13352	RB	denotes	also
T10767	13353-13357	VBN	denotes	been
T10768	13364-13368	IN	denotes	that
T10769	13414-13422	VB	denotes	interact
T10770	13369-13370	DT	denotes	a
T10771	13385-13391	NN	denotes	allele
T10772	13371-13380	JJ	denotes	recessive
T10773	13381-13384	NN	denotes	NDI
T10774	13391-13393	,	denotes	,
T10775	13393-13397	NN	denotes	AQP2
T10776	13398-13403	NN	denotes	R187C
T10777	13397-13398	HYPH	denotes	-
T10778	13403-13405	,	denotes	,
T10779	13405-13409	VBZ	denotes	does
T10780	13410-13413	RB	denotes	not
T10781	13423-13427	IN	denotes	with
T10782	13428-13432	JJ	denotes	wild
T10783	13433-13437	NN	denotes	type
T10784	13432-13433	HYPH	denotes	-
T10785	13438-13445	NN	denotes	protein
T10786	13446-13448	IN	denotes	in
T10787	13449-13456	NNS	denotes	oocytes
T10788	13457-13458	-LRB-	denotes	[
T10789	13458-13460	CD	denotes	24
T10790	13460-13461	-RRB-	denotes	]
T10791	13461-13463	,	denotes	,
T10792	13463-13466	CC	denotes	nor
T10793	13467-13471	VBZ	denotes	does
T10794	13475-13491	VB	denotes	homo-oligomerize
T10795	13472-13474	PRP	denotes	it
T10796	13492-13494	IN	denotes	in
T10797	13495-13499	NN	denotes	MDCK
T10798	13500-13505	NNS	denotes	cells
T10799	13506-13507	-LRB-	denotes	[
T10800	13507-13509	CD	denotes	22
T10801	13509-13510	-RRB-	denotes	]
T10802	13510-13511	.	denotes	.
T10803	13511-13619	sentence	denotes	Therefore, kidneys from heterozygous animals were examined for evidence of two populations of AQP2 protein.
T10804	13512-13521	RB	denotes	Therefore
T10805	13562-13570	VBN	denotes	examined
T10806	13521-13523	,	denotes	,
T10807	13523-13530	NNS	denotes	kidneys
T10808	13531-13535	IN	denotes	from
T10809	13536-13548	JJ	denotes	heterozygous
T10810	13549-13556	NNS	denotes	animals
T10811	13557-13561	VBD	denotes	were
T10812	13571-13574	IN	denotes	for
T10813	13575-13583	NN	denotes	evidence
T10814	13584-13586	IN	denotes	of
T10815	13587-13590	CD	denotes	two
T10816	13591-13602	NNS	denotes	populations
T10817	13603-13605	IN	denotes	of
T10818	13606-13610	NN	denotes	AQP2
T10819	13611-13618	NN	denotes	protein
T10820	13618-13619	.	denotes	.
T10821	13619-13775	sentence	denotes	Surprisingly, immunohistochemical staining of kidney collecting ducts from Aqp2F204V/+ mice revealed a pattern remarkably similar to wild type (Figure 5A).
T10822	13620-13632	RB	denotes	Surprisingly
T10823	13712-13720	VBD	denotes	revealed
T10824	13632-13634	,	denotes	,
T10825	13634-13653	JJ	denotes	immunohistochemical
T10826	13654-13662	NN	denotes	staining
T10827	13663-13665	IN	denotes	of
T10828	13666-13672	NN	denotes	kidney
T10829	13684-13689	NNS	denotes	ducts
T10830	13673-13683	VBG	denotes	collecting
T10831	13690-13694	IN	denotes	from
T10832	13695-13704	NN	denotes	Aqp2F204V
T10833	13707-13711	NNS	denotes	mice
T10834	13704-13705	HYPH	denotes	/
T10835	13705-13706	SYM	denotes	+
T10836	13721-13722	DT	denotes	a
T10837	13723-13730	NN	denotes	pattern
T10838	13731-13741	RB	denotes	remarkably
T10839	13742-13749	JJ	denotes	similar
T10840	13750-13752	IN	denotes	to
T10841	13753-13757	JJ	denotes	wild
T10842	13758-13762	NN	denotes	type
T10843	13763-13764	-LRB-	denotes	(
T10844	13764-13770	NN	denotes	Figure
T10845	13771-13773	CD	denotes	5A
T10846	13773-13774	-RRB-	denotes	)
T10847	13774-13775	.	denotes	.
T10848	13775-13855	sentence	denotes	AQP2 translocated completely to the apical cell surface upon dDAVP stimulation.
T10849	13776-13780	NN	denotes	AQP2
T10850	13781-13793	VBD	denotes	translocated
T10851	13794-13804	RB	denotes	completely
T10852	13805-13807	IN	denotes	to
T10853	13808-13811	DT	denotes	the
T10854	13824-13831	NN	denotes	surface
T10855	13812-13818	JJ	denotes	apical
T10856	13819-13823	NN	denotes	cell
T10857	13832-13836	IN	denotes	upon
T10858	13837-13842	NN	denotes	dDAVP
T10859	13843-13854	NN	denotes	stimulation
T10860	13854-13855	.	denotes	.
T10861	13855-14014	sentence	denotes	This wild-type staining pattern may simply reflect the fact that decreasing the amount of mutant protein by half makes it undetectable by immunocytochemistry.
T10862	13856-13860	DT	denotes	This
T10863	13880-13887	NN	denotes	pattern
T10864	13861-13865	JJ	denotes	wild
T10865	13866-13870	NN	denotes	type
T10866	13865-13866	HYPH	denotes	-
T10867	13871-13879	NN	denotes	staining
T10868	13899-13906	VB	denotes	reflect
T10869	13888-13891	MD	denotes	may
T10870	13892-13898	RB	denotes	simply
T10871	13907-13910	DT	denotes	the
T10872	13911-13915	NN	denotes	fact
T10873	13916-13920	IN	denotes	that
T10874	13969-13974	VBZ	denotes	makes
T10875	13921-13931	VBG	denotes	decreasing
T10876	13932-13935	DT	denotes	the
T10877	13936-13942	NN	denotes	amount
T10878	13943-13945	IN	denotes	of
T10879	13946-13952	NN	denotes	mutant
T10880	13953-13960	NN	denotes	protein
T10881	13961-13963	IN	denotes	by
T10882	13964-13968	NN	denotes	half
T10883	13975-13977	PRP	denotes	it
T10884	13978-13990	JJ	denotes	undetectable
T10885	13991-13993	IN	denotes	by
T10886	13994-14013	NN	denotes	immunocytochemistry
T10887	14013-14014	.	denotes	.
T10888	14014-14113	sentence	denotes	Alternatively, the presence of wild-type protein may alter the localization of the mutant protein.
T10889	14015-14028	RB	denotes	Alternatively
T10890	14068-14073	VB	denotes	alter
T10891	14028-14030	,	denotes	,
T10892	14030-14033	DT	denotes	the
T10893	14034-14042	NN	denotes	presence
T10894	14043-14045	IN	denotes	of
T10895	14046-14050	JJ	denotes	wild
T10896	14051-14055	NN	denotes	type
T10897	14050-14051	HYPH	denotes	-
T10898	14056-14063	NN	denotes	protein
T10899	14064-14067	MD	denotes	may
T10900	14074-14077	DT	denotes	the
T10901	14078-14090	NN	denotes	localization
T10902	14091-14093	IN	denotes	of
T10903	14094-14097	DT	denotes	the
T10904	14105-14112	NN	denotes	protein
T10905	14098-14104	NN	denotes	mutant
T10906	14112-14113	.	denotes	.
T10907	14113-14283	sentence	denotes	Indeed, Hendriks et al. proposed a “piggy-back” mechanism to explain the transport of nonglycosylated subunits of AQP2 to the cell surface by glycosylated subunits [22].
T10908	14114-14120	RB	denotes	Indeed
T10909	14138-14146	VBD	denotes	proposed
T10910	14120-14122	,	denotes	,
T10911	14122-14130	NNP	denotes	Hendriks
T10912	14131-14133	FW	denotes	et
T10913	14134-14137	FW	denotes	al.
T10914	14147-14148	DT	denotes	a
T10915	14162-14171	NN	denotes	mechanism
T10916	14149-14150	``	denotes	“
T10917	14150-14155	NN	denotes	piggy
T10918	14156-14160	NN	denotes	back
T10919	14155-14156	HYPH	denotes	-
T10920	14160-14161	''	denotes	”
T10921	14172-14174	TO	denotes	to
T10922	14175-14182	VB	denotes	explain
T10923	14183-14186	DT	denotes	the
T10924	14187-14196	NN	denotes	transport
T10925	14197-14199	IN	denotes	of
T10926	14200-14215	JJ	denotes	nonglycosylated
T10927	14216-14224	NNS	denotes	subunits
T10928	14225-14227	IN	denotes	of
T10929	14228-14232	NN	denotes	AQP2
T10930	14233-14235	IN	denotes	to
T10931	14236-14239	DT	denotes	the
T10932	14245-14252	NN	denotes	surface
T10933	14240-14244	NN	denotes	cell
T10934	14253-14255	IN	denotes	by
T10935	14256-14268	VBN	denotes	glycosylated
T10936	14269-14277	NNS	denotes	subunits
T10937	14278-14279	-LRB-	denotes	[
T10938	14279-14281	CD	denotes	22
T10939	14281-14282	-RRB-	denotes	]
T10940	14282-14283	.	denotes	.
T10941	14283-14448	sentence	denotes	It has also been shown that wild-type AQP2 protein can rescue a translocation-defective mutant protein, AQP2-P262L, when the two are coexpressed in MDCK cells [25].
T10942	14284-14286	PRP	denotes	It
T10943	14301-14306	VBN	denotes	shown
T10944	14287-14290	VBZ	denotes	has
T10945	14291-14295	RB	denotes	also
T10946	14296-14300	VBN	denotes	been
T10947	14307-14311	IN	denotes	that
T10948	14339-14345	VB	denotes	rescue
T10949	14312-14316	JJ	denotes	wild
T10950	14317-14321	NN	denotes	type
T10951	14316-14317	HYPH	denotes	-
T10952	14327-14334	NN	denotes	protein
T10953	14322-14326	NN	denotes	AQP2
T10954	14335-14338	MD	denotes	can
T10955	14346-14347	DT	denotes	a
T10956	14379-14386	NN	denotes	protein
T10957	14348-14361	NN	denotes	translocation
T10958	14362-14371	JJ	denotes	defective
T10959	14361-14362	HYPH	denotes	-
T10960	14372-14378	NN	denotes	mutant
T10961	14386-14388	,	denotes	,
T10962	14388-14392	NN	denotes	AQP2
T10963	14393-14398	NN	denotes	P262L
T10964	14392-14393	HYPH	denotes	-
T10965	14398-14400	,	denotes	,
T10966	14400-14404	WRB	denotes	when
T10967	14417-14428	VBN	denotes	coexpressed
T10968	14405-14408	DT	denotes	the
T10969	14409-14412	CD	denotes	two
T10970	14413-14416	VBP	denotes	are
T10971	14429-14431	IN	denotes	in
T10972	14432-14436	NN	denotes	MDCK
T10973	14437-14442	NNS	denotes	cells
T10974	14443-14444	-LRB-	denotes	[
T10975	14444-14446	CD	denotes	25
T10976	14446-14447	-RRB-	denotes	]
T10977	14447-14448	.	denotes	.
T10978	14448-16211	sentence	denotes	Figure 5 AQP2-F204V Rescue in Heterozygous Mouse Collecting Ducts and in Cotransfected MDCK Cells (A) In heterozygous animals, AQP2 localizes and responds to dDAVP normally. Immunohistochemistry was carried out on kidney sections from an Aqp2F204V/+ mouse, after injection with dDAVP. Kidney sections were sequentially immunostained for AQP2 (red) and the basolateral marker AQP3 (green). (B) Mutant and wild-type AQP2 physically interact. MDCK cells stably expressing wild-type AQP2 were transiently transfected with GFP tagged wild-type AQP2, AQP2-F204V, or GFP alone. Solubilized membranes were immunoprecipitated with a GFP antibody. Total membranes and immunoprecipitates (GFP-IP) were Western blotted using an antibody against AQP2 (arrow) or AQP2-GFP fusions (arrowhead). (C) Wild-type AQP2 rescues the localization defect of mutant AQP2. GFP fusions of either wild-type AQP2 (WT-GFP, top photomicrographs) or F204V AQP2 (F204V-GFP, bottom photomicrographs) were expressed in polarized MDCK stable cell lines expressing vector alone (vector, left photomicrographs) or AQP2-WT (right photomicrographs). Cells were stimulated with forskolin, processed for immunocytochemistry, and used to generate z-sectional images. (D) Mutant AQP2 is present at the cell surface in cells coexpressing wild-type AQP2 (AQP2-WT). GFP fused to AQP2-F204V was expressed in MDCK cells expressing wild-type AQP2 or vector alone. Cells were stimulated with forskolin, and cell surface biotinylated proteins were precipitated then analyzed for the presence of wild-type AQP2 (arrow) and AQP2-F204V (arrowhead) by Western blot. In the collecting ducts from Aqp2F204V/+ mice, the wild type may rescue the mutant protein as suggested by the subcellular distribution of AQP2 protein.
T10979	16059-16061	IN	denotes	In
T10980	16124-16130	VB	denotes	rescue
T10981	16062-16065	DT	denotes	the
T10982	16077-16082	NNS	denotes	ducts
T10983	16066-16076	VBG	denotes	collecting
T10984	16083-16087	IN	denotes	from
T10985	16088-16097	NN	denotes	Aqp2F204V
T10986	16100-16104	NNS	denotes	mice
T10987	16097-16098	HYPH	denotes	/
T10988	16098-16099	SYM	denotes	+
T10989	16104-16106	,	denotes	,
T10990	16106-16109	DT	denotes	the
T10991	16115-16119	NN	denotes	type
T10992	16110-16114	JJ	denotes	wild
T10993	16120-16123	MD	denotes	may
T10994	16131-16134	DT	denotes	the
T10995	16142-16149	NN	denotes	protein
T10996	16135-16141	NN	denotes	mutant
T10997	16150-16152	IN	denotes	as
T10998	16153-16162	VBN	denotes	suggested
T10999	16163-16165	IN	denotes	by
T11000	16166-16169	DT	denotes	the
T11001	16182-16194	NN	denotes	distribution
T11002	16170-16181	JJ	denotes	subcellular
T11003	16195-16197	IN	denotes	of
T11004	16198-16202	NN	denotes	AQP2
T11005	16203-16210	NN	denotes	protein
T11006	16210-16211	.	denotes	.
T11007	16211-16342	sentence	denotes	To test this idea, we first looked for an interaction between mutant and wild-type proteins in transfected MDCK cells (Figure 5B).
T11008	16212-16214	TO	denotes	To
T11009	16215-16219	VB	denotes	test
T11010	16240-16246	VBD	denotes	looked
T11011	16220-16224	DT	denotes	this
T11012	16225-16229	NN	denotes	idea
T11013	16229-16231	,	denotes	,
T11014	16231-16233	PRP	denotes	we
T11015	16234-16239	RB	denotes	first
T11016	16247-16250	IN	denotes	for
T11017	16251-16253	DT	denotes	an
T11018	16254-16265	NN	denotes	interaction
T11019	16266-16273	IN	denotes	between
T11020	16274-16280	NN	denotes	mutant
T11021	16295-16303	NN	denotes	proteins
T11022	16281-16284	CC	denotes	and
T11023	16285-16289	JJ	denotes	wild
T11024	16290-16294	NN	denotes	type
T11025	16289-16290	HYPH	denotes	-
T11026	16304-16306	IN	denotes	in
T11027	16307-16318	VBN	denotes	transfected
T11028	16324-16329	NNS	denotes	cells
T11029	16319-16323	NN	denotes	MDCK
T11030	16330-16331	-LRB-	denotes	(
T11031	16331-16337	NN	denotes	Figure
T11032	16338-16340	CD	denotes	5B
T11033	16340-16341	-RRB-	denotes	)
T11034	16341-16342	.	denotes	.
T11035	16342-16508	sentence	denotes	MDCK cells stably expressing wild-type AQP2 were transiently transfected with GFP expression constructs encoding GFP-tagged wild-type AQP2, AQP2-F204V, or GFP alone.
T11036	16343-16347	NN	denotes	MDCK
T11037	16348-16353	NNS	denotes	cells
T11038	16404-16415	VBN	denotes	transfected
T11039	16354-16360	RB	denotes	stably
T11040	16361-16371	VBG	denotes	expressing
T11041	16372-16376	JJ	denotes	wild
T11042	16377-16381	NN	denotes	type
T11043	16376-16377	HYPH	denotes	-
T11044	16382-16386	NN	denotes	AQP2
T11045	16387-16391	VBD	denotes	were
T11046	16392-16403	RB	denotes	transiently
T11047	16416-16420	IN	denotes	with
T11048	16421-16424	NN	denotes	GFP
T11049	16436-16446	NNS	denotes	constructs
T11050	16425-16435	NN	denotes	expression
T11051	16447-16455	VBG	denotes	encoding
T11052	16456-16459	NN	denotes	GFP
T11053	16460-16466	VBN	denotes	tagged
T11054	16459-16460	HYPH	denotes	-
T11055	16477-16481	NN	denotes	AQP2
T11056	16467-16471	JJ	denotes	wild
T11057	16472-16476	NN	denotes	type
T11058	16471-16472	HYPH	denotes	-
T11059	16481-16483	,	denotes	,
T11060	16483-16487	NN	denotes	AQP2
T11061	16488-16493	NN	denotes	F204V
T11062	16487-16488	HYPH	denotes	-
T11063	16493-16495	,	denotes	,
T11064	16495-16497	CC	denotes	or
T11065	16498-16501	NN	denotes	GFP
T11066	16502-16507	RB	denotes	alone
T11067	16507-16508	.	denotes	.
T11068	16508-16758	sentence	denotes	Antibodies against GFP coimmunoprecipitated wild-type AQP2 when AQP2-GFP or AQP2-F204V-GFP was transiently transfected, but not when GFP by itself was transiently transfected into MDCK cells stably expressing wild-type AQP2 (Figure 5B, upper blots).
T11069	16509-16519	NNS	denotes	Antibodies
T11070	16532-16552	VBD	denotes	coimmunoprecipitated
T11071	16520-16527	IN	denotes	against
T11072	16528-16531	NN	denotes	GFP
T11073	16553-16557	JJ	denotes	wild
T11074	16558-16562	NN	denotes	type
T11075	16557-16558	HYPH	denotes	-
T11076	16563-16567	NN	denotes	AQP2
T11077	16568-16572	WRB	denotes	when
T11078	16616-16627	VBN	denotes	transfected
T11079	16573-16577	NN	denotes	AQP2
T11080	16578-16581	NN	denotes	GFP
T11081	16577-16578	HYPH	denotes	-
T11082	16582-16584	CC	denotes	or
T11083	16585-16589	NN	denotes	AQP2
T11084	16596-16599	NN	denotes	GFP
T11085	16589-16590	HYPH	denotes	-
T11086	16590-16595	NN	denotes	F204V
T11087	16595-16596	HYPH	denotes	-
T11088	16600-16603	VBD	denotes	was
T11089	16604-16615	RB	denotes	transiently
T11090	16627-16629	,	denotes	,
T11091	16629-16632	CC	denotes	but
T11092	16633-16636	RB	denotes	not
T11093	16637-16641	WRB	denotes	when
T11094	16672-16683	VBN	denotes	transfected
T11095	16642-16645	NN	denotes	GFP
T11096	16646-16648	IN	denotes	by
T11097	16649-16655	PRP	denotes	itself
T11098	16656-16659	VBD	denotes	was
T11099	16660-16671	RB	denotes	transiently
T11100	16684-16688	IN	denotes	into
T11101	16689-16693	NN	denotes	MDCK
T11102	16694-16699	NNS	denotes	cells
T11103	16700-16706	RB	denotes	stably
T11104	16707-16717	VBG	denotes	expressing
T11105	16718-16722	JJ	denotes	wild
T11106	16723-16727	NN	denotes	type
T11107	16722-16723	HYPH	denotes	-
T11108	16728-16732	NN	denotes	AQP2
T11109	16733-16734	-LRB-	denotes	(
T11110	16751-16756	NNS	denotes	blots
T11111	16734-16740	NN	denotes	Figure
T11112	16741-16743	CD	denotes	5B
T11113	16743-16745	,	denotes	,
T11114	16745-16750	JJ	denotes	upper
T11115	16756-16757	-RRB-	denotes	)
T11116	16757-16758	.	denotes	.
T11117	16758-16888	sentence	denotes	Western blot of total membranes showed that wild-type AQP2 is equivalently expressed in all three cases (Figure 5B, lower blots).
T11118	16759-16766	NNP	denotes	Western
T11119	16767-16771	NN	denotes	blot
T11120	16791-16797	VBD	denotes	showed
T11121	16772-16774	IN	denotes	of
T11122	16775-16780	JJ	denotes	total
T11123	16781-16790	NNS	denotes	membranes
T11124	16798-16802	IN	denotes	that
T11125	16834-16843	VBN	denotes	expressed
T11126	16803-16807	JJ	denotes	wild
T11127	16808-16812	NN	denotes	type
T11128	16807-16808	HYPH	denotes	-
T11129	16813-16817	NN	denotes	AQP2
T11130	16818-16820	VBZ	denotes	is
T11131	16821-16833	RB	denotes	equivalently
T11132	16844-16846	IN	denotes	in
T11133	16847-16850	DT	denotes	all
T11134	16857-16862	NNS	denotes	cases
T11135	16851-16856	CD	denotes	three
T11136	16863-16864	-LRB-	denotes	(
T11137	16881-16886	NNS	denotes	blots
T11138	16864-16870	NN	denotes	Figure
T11139	16871-16873	CD	denotes	5B
T11140	16873-16875	,	denotes	,
T11141	16875-16880	JJ	denotes	lower
T11142	16886-16887	-RRB-	denotes	)
T11143	16887-16888	.	denotes	.
T11144	16888-17034	sentence	denotes	If wild-type and mutant proteins are indeed interacting in the cell, is this interaction sufficient to rescue the localization of mutant protein?
T11145	16889-16891	IN	denotes	If
T11146	16933-16944	VBG	denotes	interacting
T11147	16892-16896	JJ	denotes	wild
T11148	16897-16901	NN	denotes	type
T11149	16896-16897	HYPH	denotes	-
T11150	16913-16921	NN	denotes	proteins
T11151	16902-16905	CC	denotes	and
T11152	16906-16912	NN	denotes	mutant
T11153	16922-16925	VBP	denotes	are
T11154	16926-16932	RB	denotes	indeed
T11155	16958-16960	VBZ	denotes	is
T11156	16945-16947	IN	denotes	in
T11157	16948-16951	DT	denotes	the
T11158	16952-16956	NN	denotes	cell
T11159	16956-16958	,	denotes	,
T11160	16961-16965	DT	denotes	this
T11161	16966-16977	NN	denotes	interaction
T11162	16978-16988	JJ	denotes	sufficient
T11163	16989-16991	TO	denotes	to
T11164	16992-16998	VB	denotes	rescue
T11165	16999-17002	DT	denotes	the
T11166	17003-17015	NN	denotes	localization
T11167	17016-17018	IN	denotes	of
T11168	17019-17025	NN	denotes	mutant
T11169	17026-17033	NN	denotes	protein
T11170	17033-17034	.	denotes	?
T11171	17034-17157	sentence	denotes	To answer this question, we used MDCK cells stably transfected with wild-type AQP2 expressing vector or with empty vector.
T11172	17035-17037	TO	denotes	To
T11173	17038-17044	VB	denotes	answer
T11174	17063-17067	VBD	denotes	used
T11175	17045-17049	DT	denotes	this
T11176	17050-17058	NN	denotes	question
T11177	17058-17060	,	denotes	,
T11178	17060-17062	PRP	denotes	we
T11179	17068-17072	NN	denotes	MDCK
T11180	17073-17078	NNS	denotes	cells
T11181	17079-17085	RB	denotes	stably
T11182	17086-17097	VBN	denotes	transfected
T11183	17098-17102	IN	denotes	with
T11184	17103-17107	JJ	denotes	wild
T11185	17108-17112	NN	denotes	type
T11186	17107-17108	HYPH	denotes	-
T11187	17113-17117	NN	denotes	AQP2
T11188	17118-17128	VBG	denotes	expressing
T11189	17129-17135	NN	denotes	vector
T11190	17136-17138	CC	denotes	or
T11191	17139-17143	IN	denotes	with
T11192	17144-17149	JJ	denotes	empty
T11193	17150-17156	NN	denotes	vector
T11194	17156-17157	.	denotes	.
T11195	17157-17251	sentence	denotes	On top of these, we transiently transfected AQP2-GFP or AQP2-F204V-GFP expression constructs.
T11196	17158-17160	IN	denotes	On
T11197	17190-17201	VBD	denotes	transfected
T11198	17161-17164	NN	denotes	top
T11199	17165-17167	IN	denotes	of
T11200	17168-17173	DT	denotes	these
T11201	17173-17175	,	denotes	,
T11202	17175-17177	PRP	denotes	we
T11203	17178-17189	RB	denotes	transiently
T11204	17202-17206	NN	denotes	AQP2
T11205	17207-17210	NN	denotes	GFP
T11206	17206-17207	HYPH	denotes	-
T11207	17240-17250	NNS	denotes	constructs
T11208	17211-17213	CC	denotes	or
T11209	17214-17218	NN	denotes	AQP2
T11210	17225-17228	NN	denotes	GFP
T11211	17218-17219	HYPH	denotes	-
T11212	17219-17224	NN	denotes	F204V
T11213	17224-17225	HYPH	denotes	-
T11214	17229-17239	NN	denotes	expression
T11215	17250-17251	.	denotes	.
T11216	17251-17467	sentence	denotes	AQP2-GFP localized to the apical surface upon forskolin stimulation whether it was transiently transfected into vector-only cells (Figure 5C, upper left images) or into wild-type AQP2 cells (Figure 5C, upper right).
T11217	17252-17256	NN	denotes	AQP2
T11218	17257-17260	NN	denotes	GFP
T11219	17256-17257	HYPH	denotes	-
T11220	17261-17270	VBD	denotes	localized
T11221	17271-17273	IN	denotes	to
T11222	17274-17277	DT	denotes	the
T11223	17285-17292	NN	denotes	surface
T11224	17278-17284	JJ	denotes	apical
T11225	17293-17297	IN	denotes	upon
T11226	17298-17307	NN	denotes	forskolin
T11227	17308-17319	NN	denotes	stimulation
T11228	17320-17327	IN	denotes	whether
T11229	17347-17358	VBN	denotes	transfected
T11230	17328-17330	PRP	denotes	it
T11231	17331-17334	VBD	denotes	was
T11232	17335-17346	RB	denotes	transiently
T11233	17359-17363	IN	denotes	into
T11234	17364-17370	NN	denotes	vector
T11235	17371-17375	JJ	denotes	only
T11236	17370-17371	HYPH	denotes	-
T11237	17376-17381	NNS	denotes	cells
T11238	17382-17383	-LRB-	denotes	(
T11239	17405-17411	NNS	denotes	images
T11240	17383-17389	NN	denotes	Figure
T11241	17390-17392	CD	denotes	5C
T11242	17392-17394	,	denotes	,
T11243	17394-17399	JJ	denotes	upper
T11244	17400-17404	JJ	denotes	left
T11245	17411-17412	-RRB-	denotes	)
T11246	17413-17415	CC	denotes	or
T11247	17416-17420	IN	denotes	into
T11248	17421-17425	JJ	denotes	wild
T11249	17426-17430	NN	denotes	type
T11250	17425-17426	HYPH	denotes	-
T11251	17436-17441	NNS	denotes	cells
T11252	17431-17435	NN	denotes	AQP2
T11253	17442-17443	-LRB-	denotes	(
T11254	17443-17449	NN	denotes	Figure
T11255	17450-17452	CD	denotes	5C
T11256	17452-17454	,	denotes	,
T11257	17454-17459	JJ	denotes	upper
T11258	17460-17465	JJ	denotes	right
T11259	17465-17466	-RRB-	denotes	)
T11260	17466-17467	.	denotes	.
T11261	17467-17623	sentence	denotes	AQP2-F204V-GFP, when expressed by transient transfection into vector only cells, showed a diffuse cytoplasmic distribution pattern (Figure 5C, lower left).
T11262	17468-17472	NN	denotes	AQP2
T11263	17479-17482	NN	denotes	GFP
T11264	17472-17473	HYPH	denotes	-
T11265	17473-17478	NN	denotes	F204V
T11266	17478-17479	HYPH	denotes	-
T11267	17549-17555	VBD	denotes	showed
T11268	17482-17484	,	denotes	,
T11269	17484-17488	WRB	denotes	when
T11270	17489-17498	VBN	denotes	expressed
T11271	17499-17501	IN	denotes	by
T11272	17502-17511	JJ	denotes	transient
T11273	17512-17524	NN	denotes	transfection
T11274	17525-17529	IN	denotes	into
T11275	17530-17536	NN	denotes	vector
T11276	17537-17541	JJ	denotes	only
T11277	17542-17547	NNS	denotes	cells
T11278	17547-17549	,	denotes	,
T11279	17556-17557	DT	denotes	a
T11280	17591-17598	NN	denotes	pattern
T11281	17558-17565	JJ	denotes	diffuse
T11282	17566-17577	JJ	denotes	cytoplasmic
T11283	17578-17590	NN	denotes	distribution
T11293	17624-17628	WRB	denotes	When
T11294	17629-17638	VBN	denotes	expressed
T11295	17688-17697	VBD	denotes	localized
T11296	17639-17641	IN	denotes	in
T11297	17642-17646	JJ	denotes	wild
T11298	17647-17651	NN	denotes	type
T11299	17646-17647	HYPH	denotes	-
T11300	17657-17662	NNS	denotes	cells
T11301	17652-17656	NN	denotes	AQP2
T11302	17662-17664	,	denotes	,
T11303	17664-17671	RB	denotes	however
T11304	17671-17673	,	denotes	,
T11305	17673-17677	NN	denotes	AQP2
T11306	17684-17687	NN	denotes	GFP
T11307	17677-17678	HYPH	denotes	-
T11308	17678-17683	NN	denotes	F204V
T11309	17683-17684	HYPH	denotes	-
T11310	17698-17700	IN	denotes	to
T11311	17701-17704	DT	denotes	the
T11312	17712-17719	NN	denotes	surface
T11313	17705-17711	JJ	denotes	apical
T11314	17720-17722	IN	denotes	to
T11315	17723-17730	VBG	denotes	varying
T11316	17731-17738	NNS	denotes	degrees
T11317	17739-17740	-LRB-	denotes	(
T11318	17763-17769	NNS	denotes	images
T11319	17740-17746	NN	denotes	Figure
T11320	17747-17749	CD	denotes	5C
T11321	17749-17751	,	denotes	,
T11322	17751-17756	JJ	denotes	lower
T11323	17757-17762	JJ	denotes	right
T11324	17770-17771	-LRB-	denotes	[
T11325	17771-17772	CD	denotes	i
T11326	17772-17773	SYM	denotes	–
T11327	17773-17776	CD	denotes	iii
T11328	17776-17777	-RRB-	denotes	]
T11329	17777-17778	-RRB-	denotes	)
T11330	17778-17779	.	denotes	.
T11331	17779-17861	sentence	denotes	The lower right images of Figure 5C shows three cells from a single transfection.
T11332	17780-17783	DT	denotes	The
T11333	17796-17802	NNS	denotes	images
T11334	17784-17789	JJ	denotes	lower
T11335	17790-17795	JJ	denotes	right
T11336	17816-17821	VBZ	denotes	shows
T11337	17803-17805	IN	denotes	of
T11338	17806-17812	NN	denotes	Figure
T11339	17813-17815	CD	denotes	5C
T11340	17822-17827	CD	denotes	three
T11341	17828-17833	NNS	denotes	cells
T11342	17834-17838	IN	denotes	from
T11343	17839-17840	DT	denotes	a
T11344	17848-17860	NN	denotes	transfection
T11345	17841-17847	JJ	denotes	single
T11346	17860-17861	.	denotes	.
T11347	17861-18009	sentence	denotes	The first is a nontransfected cell that shows the localization of the stably expressing wild-type AQP2, which is apical upon forskolin stimulation.
T11348	17862-17865	DT	denotes	The
T11349	17866-17871	JJ	denotes	first
T11350	17872-17874	VBZ	denotes	is
T11351	17875-17876	DT	denotes	a
T11352	17892-17896	NN	denotes	cell
T11353	17877-17891	JJ	denotes	nontransfected
T11354	17897-17901	WDT	denotes	that
T11355	17902-17907	VBZ	denotes	shows
T11356	17908-17911	DT	denotes	the
T11357	17912-17924	NN	denotes	localization
T11358	17925-17927	IN	denotes	of
T11359	17928-17931	DT	denotes	the
T11360	17960-17964	NN	denotes	AQP2
T11361	17932-17938	RB	denotes	stably
T11362	17939-17949	VBG	denotes	expressing
T11363	17950-17954	JJ	denotes	wild
T11364	17955-17959	NN	denotes	type
T11365	17954-17955	HYPH	denotes	-
T11366	17964-17966	,	denotes	,
T11367	17966-17971	WDT	denotes	which
T11368	17972-17974	VBZ	denotes	is
T11369	17975-17981	JJ	denotes	apical
T11370	17982-17986	IN	denotes	upon
T11371	17987-17996	NN	denotes	forskolin
T11372	17997-18008	NN	denotes	stimulation
T11373	18008-18009	.	denotes	.
T11374	18009-18106	sentence	denotes	The next two show expression of both the stable wild-type AQP2 and the transient AQP2-F204V-GFP.
T11375	18010-18013	DT	denotes	The
T11376	18019-18022	CD	denotes	two
T11377	18014-18018	JJ	denotes	next
T11378	18023-18027	VBP	denotes	show
T11379	18028-18038	NN	denotes	expression
T11380	18039-18041	IN	denotes	of
T11381	18042-18046	CC	denotes	both
T11382	18068-18072	NN	denotes	AQP2
T11383	18047-18050	DT	denotes	the
T11384	18051-18057	JJ	denotes	stable
T11385	18058-18062	JJ	denotes	wild
T11386	18063-18067	NN	denotes	type
T11387	18062-18063	HYPH	denotes	-
T11388	18073-18076	CC	denotes	and
T11389	18077-18080	DT	denotes	the
T11390	18102-18105	NN	denotes	GFP
T11391	18081-18090	JJ	denotes	transient
T11392	18091-18095	NN	denotes	AQP2
T11393	18095-18096	HYPH	denotes	-
T11394	18096-18101	NN	denotes	F204V
T11395	18101-18102	HYPH	denotes	-
T11396	18105-18106	.	denotes	.
T11397	18106-18239	sentence	denotes	In cell (ii), localization of wild-type AQP2 was indistinguishable from AQP2-F204V-GFP; both were apical upon forskolin stimulation.
T11398	18107-18109	IN	denotes	In
T11399	18152-18155	VBD	denotes	was
T11400	18110-18114	NN	denotes	cell
T11401	18115-18116	-LRB-	denotes	(
T11402	18116-18118	CD	denotes	ii
T11403	18118-18119	-RRB-	denotes	)
T11404	18119-18121	,	denotes	,
T11405	18121-18133	NN	denotes	localization
T11406	18134-18136	IN	denotes	of
T11407	18137-18141	JJ	denotes	wild
T11408	18142-18146	NN	denotes	type
T11409	18141-18142	HYPH	denotes	-
T11410	18147-18151	NN	denotes	AQP2
T11411	18200-18204	VBD	denotes	were
T11412	18156-18173	JJ	denotes	indistinguishable
T11413	18174-18178	IN	denotes	from
T11414	18179-18183	NN	denotes	AQP2
T11415	18190-18193	NN	denotes	GFP
T11416	18183-18184	HYPH	denotes	-
T11417	18184-18189	NN	denotes	F204V
T11418	18189-18190	HYPH	denotes	-
T11419	18193-18194	:	denotes	;
T11420	18195-18199	DT	denotes	both
T11421	18205-18211	JJ	denotes	apical
T11422	18212-18216	IN	denotes	upon
T11423	18217-18226	NN	denotes	forskolin
T11424	18227-18238	NN	denotes	stimulation
T11425	18238-18239	.	denotes	.
T11426	18239-18344	sentence	denotes	Although the effect was subtle in cell (iii), AQP2-F204V-GFP was partly localized to the apical surface.
T11427	18240-18248	IN	denotes	Although
T11428	18260-18263	VBD	denotes	was
T11429	18249-18252	DT	denotes	the
T11430	18253-18259	NN	denotes	effect
T11431	18312-18321	VBN	denotes	localized
T11432	18264-18270	JJ	denotes	subtle
T11433	18271-18273	IN	denotes	in
T11434	18274-18278	NN	denotes	cell
T11435	18279-18280	-LRB-	denotes	(
T11436	18280-18283	CD	denotes	iii
T11437	18283-18284	-RRB-	denotes	)
T11438	18284-18286	,	denotes	,
T11439	18286-18290	NN	denotes	AQP2
T11440	18297-18300	NN	denotes	GFP
T11441	18290-18291	HYPH	denotes	-
T11442	18291-18296	NN	denotes	F204V
T11443	18296-18297	HYPH	denotes	-
T11444	18301-18304	VBD	denotes	was
T11445	18305-18311	RB	denotes	partly
T11446	18322-18324	IN	denotes	to
T11447	18325-18328	DT	denotes	the
T11448	18336-18343	NN	denotes	surface
T11449	18329-18335	JJ	denotes	apical
T11450	18343-18344	.	denotes	.
T11451	18344-18454	sentence	denotes	Generally, the localization of AQP2-F204V-GFP was clearly more apical when wild-type AQP2 was also expressed.
T11452	18345-18354	RB	denotes	Generally
T11453	18391-18394	VBD	denotes	was
T11454	18354-18356	,	denotes	,
T11455	18356-18359	DT	denotes	the
T11456	18360-18372	NN	denotes	localization
T11457	18373-18375	IN	denotes	of
T11458	18376-18380	NN	denotes	AQP2
T11459	18387-18390	NN	denotes	GFP
T11460	18380-18381	HYPH	denotes	-
T11461	18381-18386	NN	denotes	F204V
T11462	18386-18387	HYPH	denotes	-
T11463	18395-18402	RB	denotes	clearly
T11464	18403-18407	RBR	denotes	more
T11465	18408-18414	JJ	denotes	apical
T11466	18415-18419	WRB	denotes	when
T11467	18444-18453	VBN	denotes	expressed
T11468	18420-18424	JJ	denotes	wild
T11469	18425-18429	NN	denotes	type
T11470	18424-18425	HYPH	denotes	-
T11471	18430-18434	NN	denotes	AQP2
T11472	18435-18438	VBD	denotes	was
T11473	18439-18443	RB	denotes	also
T11474	18453-18454	.	denotes	.
T11475	18454-18687	sentence	denotes	To confirm these results biochemically, we transfected the same cell lines (wild-type AQP2 or vector) with F204V-GFP, biotinylated surface proteins after forskolin stimulation, and precipitated the biotinylated proteins (Figure 5D).
T11476	18455-18457	TO	denotes	To
T11477	18458-18465	VB	denotes	confirm
T11478	18498-18509	VBD	denotes	transfected
T11479	18466-18471	DT	denotes	these
T11480	18472-18479	NNS	denotes	results
T11481	18480-18493	RB	denotes	biochemically
T11482	18493-18495	,	denotes	,
T11483	18495-18497	PRP	denotes	we
T11484	18510-18513	DT	denotes	the
T11485	18524-18529	NNS	denotes	lines
T11486	18514-18518	JJ	denotes	same
T11487	18519-18523	NN	denotes	cell
T11488	18530-18531	-LRB-	denotes	(
T11489	18531-18535	JJ	denotes	wild
T11490	18536-18540	NN	denotes	type
T11491	18535-18536	HYPH	denotes	-
T11492	18541-18545	NN	denotes	AQP2
T11493	18546-18548	CC	denotes	or
T11494	18549-18555	NN	denotes	vector
T11495	18555-18556	-RRB-	denotes	)
T11496	18557-18561	IN	denotes	with
T11497	18562-18567	NN	denotes	F204V
T11498	18568-18571	NN	denotes	GFP
T11499	18567-18568	HYPH	denotes	-
T11500	18571-18573	,	denotes	,
T11501	18573-18585	VBD	denotes	biotinylated
T11502	18586-18593	NN	denotes	surface
T11503	18594-18602	NN	denotes	proteins
T11504	18603-18608	IN	denotes	after
T11505	18609-18618	NN	denotes	forskolin
T11506	18619-18630	NN	denotes	stimulation
T11507	18630-18632	,	denotes	,
T11508	18632-18635	CC	denotes	and
T11509	18636-18648	VBD	denotes	precipitated
T11510	18649-18652	DT	denotes	the
T11511	18666-18674	NN	denotes	proteins
T11512	18653-18665	VBN	denotes	biotinylated
T11513	18675-18676	-LRB-	denotes	(
T11514	18676-18682	NN	denotes	Figure
T11515	18683-18685	CD	denotes	5D
T11516	18685-18686	-RRB-	denotes	)
T11517	18686-18687	.	denotes	.
T11518	18687-18880	sentence	denotes	AQP2-F204V-GFP is expressed approximately equally in both cell lines (Figure 5D, total cells), but is biotinylated only when wild-type AQP2 is also expressed (Figure 5D, surface biotinylated).
T11519	18688-18692	NN	denotes	AQP2
T11520	18699-18702	NN	denotes	GFP
T11521	18692-18693	HYPH	denotes	-
T11522	18693-18698	NN	denotes	F204V
T11523	18698-18699	HYPH	denotes	-
T11524	18706-18715	VBN	denotes	expressed
T11525	18703-18705	VBZ	denotes	is
T11526	18716-18729	RB	denotes	approximately
T11527	18730-18737	RB	denotes	equally
T11528	18738-18740	IN	denotes	in
T11529	18741-18745	DT	denotes	both
T11530	18751-18756	NNS	denotes	lines
T11531	18746-18750	NN	denotes	cell
T11532	18757-18758	-LRB-	denotes	(
T11533	18775-18780	NNS	denotes	cells
T11534	18758-18764	NN	denotes	Figure
T11535	18765-18767	CD	denotes	5D
T11536	18767-18769	,	denotes	,
T11537	18769-18774	JJ	denotes	total
T11538	18780-18781	-RRB-	denotes	)
T11539	18781-18783	,	denotes	,
T11540	18783-18786	CC	denotes	but
T11541	18787-18789	VBZ	denotes	is
T11542	18790-18802	VBN	denotes	biotinylated
T11543	18803-18807	RB	denotes	only
T11544	18836-18845	VBN	denotes	expressed
T11545	18808-18812	WRB	denotes	when
T11546	18813-18817	JJ	denotes	wild
T11547	18818-18822	NN	denotes	type
T11548	18817-18818	HYPH	denotes	-
T11549	18823-18827	NN	denotes	AQP2
T11550	18828-18830	VBZ	denotes	is
T11551	18831-18835	RB	denotes	also
T11552	18846-18847	-LRB-	denotes	(
T11553	18847-18853	NN	denotes	Figure
T11554	18854-18856	CD	denotes	5D
T11555	18856-18858	,	denotes	,
T11556	18858-18865	NN	denotes	surface
T11557	18866-18878	VBN	denotes	biotinylated
T11558	18878-18879	-RRB-	denotes	)
T11559	18879-18880	.	denotes	.
T11560	18880-19065	sentence	denotes	Since only cell surface proteins are accessible to biotin, these results indicate that AQP2-F204V is transported to the cell surface when wild-type AQP2 is present, but not on its own.
T11561	18881-18886	IN	denotes	Since
T11562	18914-18917	VBP	denotes	are
T11563	18887-18891	RB	denotes	only
T11564	18905-18913	NN	denotes	proteins
T11565	18892-18896	NN	denotes	cell
T11566	18897-18904	NN	denotes	surface
T11567	18954-18962	VBP	denotes	indicate
T11568	18918-18928	JJ	denotes	accessible
T11569	18929-18931	IN	denotes	to
T11570	18932-18938	NN	denotes	biotin
T11571	18938-18940	,	denotes	,
T11572	18940-18945	DT	denotes	these
T11573	18946-18953	NNS	denotes	results
T11574	18963-18967	IN	denotes	that
T11575	18982-18993	VBN	denotes	transported
T11576	18968-18972	NN	denotes	AQP2
T11577	18973-18978	NN	denotes	F204V
T11578	18972-18973	HYPH	denotes	-
T11579	18979-18981	VBZ	denotes	is
T11580	18994-18996	IN	denotes	to
T11581	18997-19000	DT	denotes	the
T11582	19006-19013	NN	denotes	surface
T11583	19001-19005	NN	denotes	cell
T11584	19014-19018	WRB	denotes	when
T11585	19034-19036	VBZ	denotes	is
T11586	19019-19023	JJ	denotes	wild
T11587	19024-19028	NN	denotes	type
T11588	19023-19024	HYPH	denotes	-
T11589	19029-19033	NN	denotes	AQP2
T11590	19037-19044	JJ	denotes	present
T11591	19044-19046	,	denotes	,
T11592	19046-19049	CC	denotes	but
T11593	19050-19053	RB	denotes	not
T11594	19054-19056	IN	denotes	on
T11595	19057-19060	PRP$	denotes	its
T11596	19061-19064	JJ	denotes	own
T11597	19064-19065	.	denotes	.
R916	T8980	T8981	punct	[,20
R917	T8951	T8952	prep	In,found
R918	T8953	T8954	det	a,screen
R919	T8954	T8951	pobj	screen,In
R920	T8955	T8956	amod	forward,genetic
R921	T8956	T8954	amod	genetic,screen
R922	T8957	T8958	dep	that,used
R923	T8958	T8954	relcl	used,screen
R924	T8959	T8958	dobj	ethylnitrosourea,used
R925	T8960	T8959	punct	(,ethylnitrosourea
R926	T8961	T8959	appos	ENU,ethylnitrosourea
R927	T8981	T8958	parataxis	20,used
R928	T8962	T8958	punct	),used
R929	T8963	T8964	aux	to,induce
R930	T8964	T8958	advcl	induce,used
R931	T8982	T8981	nummod	19,20
R932	T8965	T8964	dobj	mutations,induce
R933	T8966	T8964	prep	in,induce
R934	T8967	T8968	det	a,animal
R935	T8983	T8981	punct	",",20
R936	T8968	T8966	pobj	animal,in
R937	T8969	T8968	compound	founder,animal
R938	T8970	T8971	poss	whose,offspring
R939	T8984	T8981	punct	],20
R940	T8971	T8972	dep	offspring,screened
R941	T8972	T8968	relcl	screened,animal
R942	T8973	T8972	auxpass	were,screened
R943	T8985	T8952	punct	", ",found
R944	T8974	T8972	advmod	then,screened
R945	T8986	T8952	nsubj	we,found
R946	T8975	T8972	prep	for,screened
R947	T8976	T8977	amod	abnormal,metabolism
R948	T8987	T8988	det	a,family
R949	T8977	T8975	pobj	metabolism,for
R950	T8978	T8979	amod	whole,body
R951	T8988	T8952	dobj	family,found
R952	T8979	T8977	compound	body,metabolism
R953	T8989	T8988	prep	of,family
R954	T8990	T8989	pobj	mice,of
R955	T8991	T8992	dep	that,urinated
R956	T9086	T9084	pobj	substitution,to
R957	T9087	T9086	nmod	valine,substitution
R958	T9088	T9087	prep	for,valine
R959	T9089	T9088	pobj	phenylalanine,for
R960	T9090	T9086	prep	at,substitution
R961	T8992	T8988	relcl	urinated,family
R962	T9091	T9092	compound	amino,acid
R963	T9092	T9090	pobj	acid,at
R964	T9093	T9092	nummod	204,acid
R965	T9094	T9092	prep	of,acid
R966	T8993	T8992	cc	and,urinated
R967	T9095	T9096	det	the,protein
R968	T9096	T9094	pobj	protein,of
R969	T9097	T9092	punct	(,acid
R970	T8994	T8992	conj	drank,urinated
R971	T9098	T9092	appos	F204V,acid
R972	T9099	T9092	punct	),acid
R973	T9100	T9056	punct	.,identified
R974	T8995	T8994	advmod	excessively,drank
R975	T8996	T8952	punct	.,found
R976	T9102	T9103	nsubj	AQP2,is
R977	T9104	T9105	det	a,channel
R978	T8998	T8999	nmod	Serum,analysis
R979	T9105	T9103	attr	channel,is
R980	T9106	T9107	nummod	six,transmembrane
R981	T9107	T9105	compound	transmembrane,channel
R982	T9108	T9107	punct	-,transmembrane
R983	T8999	T9002	nsubj	analysis,showed
R984	T9109	T9105	compound	water,channel
R985	T9110	T9103	punct	", ",is
R986	T9000	T8998	cc	and,Serum
R987	T9111	T9103	cc	and,is
R988	T9112	T9113	nsubj	F204,lies
R989	T9113	T9103	conj	lies,is
R990	T9001	T8998	conj	urine,Serum
R991	T9114	T9113	prep	near,lies
R992	T9115	T9116	det	the,face
R993	T9003	T9004	mark	that,were
R994	T9116	T9114	pobj	face,near
R995	T9117	T9116	amod	extracellular,face
R996	T9118	T9116	prep	of,face
R997	T9119	T9120	det	the,domain
R998	T9120	T9118	pobj	domain,of
R999	T9004	T9002	ccomp	were,showed
R1000	T9121	T9120	amod	sixth,domain
R1001	T9005	T9006	compound	plasma,levels
R1002	T9122	T9123	npadvmod	membrane,spanning
R1003	T9123	T9120	amod	spanning,domain
R1004	T9124	T9120	punct	", ",domain
R1005	T9125	T9126	det	a,region
R1006	T9006	T9004	nsubj	levels,were
R1007	T9126	T9120	appos	region,domain
R1008	T9127	T9126	amod	rich,region
R1009	T9128	T9127	prep	in,rich
R1010	T9129	T9130	amod	hydrophobic,acids
R1011	T9130	T9128	pobj	acids,in
R1012	T9131	T9130	compound	amino,acids
R1013	T9007	T9006	compound	glucose,levels
R1014	T9132	T9113	punct	.,lies
R1015	T9134	T9135	nsubjpass	This,conserved
R1016	T9008	T9004	acomp	normal,were
R1017	T9136	T9134	cc	and,This
R1018	T9137	T9138	det	the,other
R1019	T9009	T9004	cc	and,were
R1020	T9138	T9139	nmod	other,domains
R1021	T9139	T9134	conj	domains,This
R1022	T9140	T9141	npadvmod	membrane,spanning
R1023	T9010	T9011	expl	there,was
R1024	T9141	T9139	amod	spanning,domains
R1025	T9142	T9141	punct	-,spanning
R1026	T9011	T9004	conj	was,were
R1027	T9143	T9135	auxpass	are,conserved
R1028	T9144	T9135	prep	among,conserved
R1029	T9145	T9146	amod	vertebrate,species
R1030	T9012	T9013	det	no,glucose
R1031	T9146	T9144	pobj	species,among
R1032	T9147	T9135	punct	.,conserved
R1033	T9013	T9011	attr	glucose,was
R1034	T9149	T9150	det	The,phenylalanine
R1035	T9150	T9151	nsubj	phenylalanine,is
R1036	T9014	T9011	prep	in,was
R1037	T9152	T9150	prep	at,phenylalanine
R1038	T9015	T9016	det	the,urine
R1039	T9016	T9014	pobj	urine,in
R1040	T9153	T9152	pobj	position,at
R1041	T9154	T9153	nummod	204,position
R1042	T9017	T9018	punct	(,data
R1043	T9155	T9156	advmod	particularly,conserved
R1044	T9156	T9151	acomp	conserved,is
R1045	T9018	T9002	meta	data,showed
R1046	T9157	T9156	advmod	well,conserved
R1047	T9158	T9159	punct	(,Figure
R1048	T9159	T9156	parataxis	Figure,conserved
R1049	T9019	T9018	amod	unpublished,data
R1050	T9160	T9159	nummod	1B,Figure
R1051	T9161	T9159	punct	),Figure
R1052	T9020	T9018	punct	),data
R1053	T9162	T9151	punct	", ",is
R1054	T9163	T9164	preconj	not,among
R1055	T9164	T9151	prep	among,is
R1056	T9021	T9002	punct	.,showed
R1057	T9165	T9163	advmod	only,not
R1058	T9166	T9167	amod	vertebrate,proteins
R1059	T9023	T9024	advmod	Hence,was
R1060	T9167	T9164	pobj	proteins,among
R1061	T9168	T9167	compound	AQP2,proteins
R1062	T9169	T9164	punct	", ",among
R1063	T9170	T9164	cc	but,among
R1064	T9171	T9170	advmod	also,but
R1065	T9172	T9164	conj	among,among
R1066	T9173	T9174	compound	others,members
R1067	T9025	T9024	punct	", ",was
R1068	T9174	T9172	pobj	members,among
R1069	T9175	T9174	prep	of,members
R1070	T9176	T9177	det	this,family
R1071	T9177	T9175	pobj	family,of
R1072	T9178	T9151	punct	.,is
R1073	T9026	T9024	nsubj	this,was
R1074	T9180	T9181	compound	Aqp2F204V,F204V
R1075	T9181	T9183	compound	F204V,mice
R1076	T9027	T9028	det	an,example
R1077	T9182	T9181	punct	/,F204V
R1078	T9183	T9184	nsubj	mice,have
R1079	T9028	T9024	attr	example,was
R1080	T9185	T9186	advmod	dramatically,increased
R1081	T9186	T9187	amod	increased,production
R1082	T9029	T9028	prep	of,example
R1083	T9187	T9184	dobj	production,have
R1084	T9188	T9187	compound	urine,production
R1085	T9189	T9184	punct	", ",have
R1086	T9030	T9031	compound	diabetes,insipidus
R1087	T9190	T9191	prep	in,producing
R1088	T9191	T9184	advcl	producing,have
R1089	T9031	T9029	pobj	insipidus,of
R1090	T9032	T9024	punct	.,was
R1091	T9034	T9035	det	The,disorder
R1092	T9192	T9193	det	some,cases
R1093	T9193	T9190	pobj	cases,in
R1094	T9194	T9195	det	an,amount
R1095	T9035	T9036	nsubj	disorder,segregated
R1096	T9195	T9191	dobj	amount,producing
R1097	T9196	T9195	prep	of,amount
R1098	T9197	T9196	pobj	urine,of
R1099	T9037	T9035	prep	in,disorder
R1100	T9198	T9191	prep	in,producing
R1101	T9199	T9200	nummod	24,h
R1102	T9200	T9198	pobj	h,in
R1103	T9201	T9202	dep	that,exceeds
R1104	T9038	T9039	det	these,mice
R1105	T9202	T9191	ccomp	exceeds,producing
R1106	T9203	T9204	poss	their,weight
R1107	T9204	T9202	dobj	weight,exceeds
R1108	T9039	T9037	pobj	mice,in
R1109	T9205	T9204	compound	body,weight
R1110	T9206	T9191	punct	", ",producing
R1111	T9040	T9036	prep	in,segregated
R1112	T9207	T9191	prep	compared,producing
R1113	T9208	T9207	prep	to,compared
R1114	T9209	T9210	poss	their,littermates
R1115	T9210	T9208	pobj	littermates,to
R1116	T9211	T9210	amod	heterozygous,littermates
R1117	T9212	T9211	cc	or,heterozygous
R1118	T9041	T9042	det	a,manner
R1119	T9213	T9214	amod	wild,type
R1120	T9214	T9211	conj	type,heterozygous
R1121	T9215	T9214	punct	-,type
R1122	T9042	T9040	pobj	manner,in
R1123	T9216	T9184	punct	.,have
R1124	T9043	T9042	amod	monogenic,manner
R1125	T9218	T9219	amod	Such,loss
R1126	T9219	T9220	nsubj	loss,lead
R1127	T9044	T9042	punct	", ",manner
R1128	T9221	T9219	prep	of,loss
R1129	T9222	T9221	pobj	water,of
R1130	T9223	T9220	aux	would,lead
R1131	T9224	T9220	advmod	rapidly,lead
R1132	T9045	T9046	amod	autosomal,recessive
R1133	T9225	T9220	prep	to,lead
R1134	T9226	T9225	pobj	dehydration,to
R1135	T9227	T9228	auxpass	were,compensated
R1136	T9046	T9042	amod	recessive,manner
R1137	T9228	T9220	advcl	compensated,lead
R1138	T9229	T9228	nsubjpass	it,compensated
R1139	T9047	T9036	punct	", ",segregated
R1140	T9230	T9228	neg	not,compensated
R1141	T9231	T9228	agent	by,compensated
R1142	T9232	T9233	amod	increased,intake
R1143	T9048	T9036	advcl	making,segregated
R1144	T9233	T9231	pobj	intake,by
R1145	T9234	T9233	compound	water,intake
R1146	T9235	T9220	punct	.,lead
R1147	T9049	T9050	nsubj	Aqp2,gene
R1148	T9237	T9238	advmod	Indeed,increase
R1149	T9050	T9048	ccomp	gene,making
R1150	T9239	T9238	punct	", ",increase
R1151	T9051	T9050	det	a,gene
R1152	T9240	T9241	compound	mutant,mice
R1153	T9052	T9050	compound	candidate,gene
R1154	T9241	T9238	nsubj	mice,increase
R1155	T9242	T9238	advmod	also,increase
R1156	T9053	T9036	punct	.,segregated
R1157	T9243	T9238	advmod	dramatically,increase
R1158	T9244	T9245	poss	their,intake
R1159	T9245	T9238	dobj	intake,increase
R1160	T9055	T9056	nsubj	Sequencing,identified
R1161	T9246	T9245	compound	water,intake
R1162	T9247	T9248	punct	(,Figure
R1163	T9248	T9238	parataxis	Figure,increase
R1164	T9249	T9248	nummod	1C,Figure
R1165	T9057	T9055	prep	of,Sequencing
R1166	T9250	T9248	punct	),Figure
R1167	T9251	T9238	prep	compared,increase
R1168	T9252	T9251	prep	to,compared
R1169	T9253	T9254	poss	their,littermates
R1170	T9254	T9252	pobj	littermates,to
R1171	T9058	T9059	compound	Aqp2,region
R1172	T9255	T9254	amod	heterozygous,littermates
R1173	T9256	T9255	cc	or,heterozygous
R1174	T9257	T9258	amod	wild,type
R1175	T9059	T9057	pobj	region,of
R1176	T9258	T9255	conj	type,heterozygous
R1177	T9259	T9258	punct	-,type
R1178	T9260	T9238	punct	.,increase
R1179	T9060	T9059	compound	coding,region
R1180	T9262	T9263	det	This,phenotype
R1181	T9061	T9059	prep	of,region
R1182	T9263	T9264	nsubj	phenotype,showed
R1183	T9265	T9263	punct	—,phenotype
R1184	T9062	T9063	amod	affected,mice
R1185	T9266	T9267	amod	increased,output
R1186	T9267	T9263	appos	output,phenotype
R1187	T9268	T9267	amod	urinary,output
R1188	T9063	T9061	pobj	mice,of
R1189	T9269	T9267	cc	and,output
R1190	T9270	T9271	compound	water,intake
R1191	T9271	T9267	conj	intake,output
R1192	T9064	T9065	det	a,transversion
R1193	T9272	T9264	punct	—,showed
R1194	T9273	T9274	amod	complete,concordance
R1195	T9274	T9264	dobj	concordance,showed
R1196	T9065	T9056	dobj	transversion,identified
R1197	T9275	T9274	prep	with,concordance
R1198	T9276	T9275	pobj	homozygosity,with
R1199	T9277	T9276	prep	of,homozygosity
R1200	T9066	T9065	nmod	thymine,transversion
R1201	T9278	T9279	det	the,mutation
R1202	T9279	T9277	pobj	mutation,of
R1203	T9280	T9279	compound	F204V,mutation
R1204	T9067	T9066	prep	to,thymine
R1205	T9281	T9264	prep	in,showed
R1206	T9282	T9283	det	the,animals
R1207	T9283	T9281	pobj	animals,in
R1208	T9068	T9067	pobj	guanine,to
R1209	T9284	T9283	nummod	58,animals
R1210	T9285	T9283	acl	tested,animals
R1211	T9286	T9264	punct	.,showed
R1212	T9069	T9066	punct	(,thymine
R1213	T9288	T9289	compound	Diabetes,insipidus
R1214	T9289	T9290	nsubjpass	insipidus,defined
R1215	T9070	T9066	appos	T,thymine
R1216	T9291	T9290	aux	can,defined
R1217	T9292	T9290	auxpass	be,defined
R1218	T9293	T9290	prep	as,defined
R1219	T9294	T9295	det	an,inability
R1220	T9295	T9293	pobj	inability,as
R1221	T9071	T9070	prep	to,T
R1222	T9296	T9297	aux	to,concentrate
R1223	T9297	T9295	acl	concentrate,inability
R1224	T9072	T9071	pobj	G,to
R1225	T9073	T9065	punct	),transversion
R1226	T9074	T9075	punct	(,Figure
R1227	T9298	T9297	dobj	urine,concentrate
R1228	T9299	T9300	advmod	where,appropriate
R1229	T9075	T9065	parataxis	Figure,transversion
R1230	T9300	T9297	advcl	appropriate,concentrate
R1231	T9301	T9290	punct	.,defined
R1232	T9076	T9075	nummod	1A,Figure
R1233	T9303	T9304	prep	Compared,produce
R1234	T9305	T9303	prep	to,Compared
R1235	T9077	T9075	punct	),Figure
R1236	T9306	T9307	amod	wild,type
R1237	T9307	T9309	nmod	type,littermates
R1238	T9308	T9307	punct	-,type
R1239	T9078	T9065	punct	", ",transversion
R1240	T9309	T9305	pobj	littermates,to
R1241	T9310	T9307	cc	or,type
R1242	T9311	T9307	conj	heterozygous,type
R1243	T9079	T9080	dep	which,predicted
R1244	T9312	T9304	punct	", ",produce
R1245	T9313	T9314	compound	Aqp2F204V,F204V
R1246	T9314	T9316	compound	F204V,mice
R1247	T9080	T9065	relcl	predicted,transversion
R1248	T9315	T9314	punct	/,F204V
R1249	T9316	T9304	nsubj	mice,produce
R1250	T9317	T9318	advmod	very,dilute
R1251	T9318	T9319	amod	dilute,urine
R1252	T9081	T9080	auxpass	is,predicted
R1253	T9319	T9304	dobj	urine,produce
R1254	T9320	T9321	punct	(,Figure
R1255	T9321	T9304	parataxis	Figure,produce
R1256	T9082	T9083	aux	to,lead
R1257	T9322	T9321	nummod	1D,Figure
R1258	T9323	T9321	punct	),Figure
R1259	T9083	T9080	xcomp	lead,predicted
R1260	T9324	T9304	punct	.,produce
R1261	T9326	T9327	amod	Basal,concentration
R1262	T9084	T9083	prep	to,lead
R1263	T9085	T9086	det	a,substitution
R1264	T9327	T9329	nsubj	concentration,is
R1265	T9403	T9402	oprd	desmopressin,called
R1266	T9328	T9327	compound	urine,concentration
R1267	T9330	T9327	prep	in,concentration
R1268	T9331	T9332	compound	mutant,mice
R1269	T9332	T9330	pobj	mice,in
R1270	T9333	T9334	advmod	about,161
R1271	T9404	T9383	punct	),analog
R1272	T9334	T9335	nummod	161,mOsm
R1273	T9335	T9329	attr	mOsm,is
R1274	T9405	T9386	punct	", ",is
R1275	T9336	T9335	punct	", ",mOsm
R1276	T9337	T9335	prep	compared,mOsm
R1277	T9338	T9337	prep	to,compared
R1278	T9406	T9407	det	a,agonist
R1279	T9339	T9340	quantmod	about,"1,293"
R1280	T9340	T9341	nummod	"1,293",mOsm
R1281	T9341	T9338	pobj	mOsm,to
R1282	T9407	T9386	attr	agonist,is
R1283	T9342	T9341	prep	in,mOsm
R1284	T9343	T9344	amod	wild,type
R1285	T9344	T9346	compound	type,mice
R1286	T9408	T9407	amod	potent,agonist
R1287	T9345	T9344	punct	-,type
R1288	T9346	T9342	pobj	mice,in
R1289	T9409	T9407	prep	of,agonist
R1290	T9347	T9348	punct	(,0.001
R1291	T9348	T9329	parataxis	0.001,is
R1292	T9349	T9348	nsubj	p,0.001
R1293	T9410	T9409	pobj	AVPR2,of
R1294	T9350	T9348	punct	<,0.001
R1295	T9351	T9348	punct	),0.001
R1296	T9352	T9329	punct	.,is
R1297	T9411	T9386	punct	.,is
R1298	T9354	T9355	advmod	Normally,is
R1299	T9413	T9414	advmod	When,administered
R1300	T9356	T9355	punct	", ",is
R1301	T9357	T9358	compound	urine,concentration
R1302	T9358	T9355	nsubj	concentration,is
R1303	T9359	T9355	prep	under,is
R1304	T9360	T9361	det	the,control
R1305	T9361	T9359	pobj	control,under
R1306	T9414	T9415	advcl	administered,leads
R1307	T9362	T9361	prep	of,control
R1308	T9363	T9364	det	the,hypothalamus
R1309	T9364	T9362	pobj	hypothalamus,of
R1310	T9365	T9364	punct	", ",hypothalamus
R1311	T9416	T9414	prep	to,administered
R1312	T9366	T9367	dep	which,secretes
R1313	T9367	T9364	relcl	secretes,hypothalamus
R1314	T9368	T9367	punct	", ",secretes
R1315	T9369	T9367	prep	in,secretes
R1316	T9417	T9418	amod	wild,type
R1317	T9370	T9369	pobj	response,in
R1318	T9371	T9370	prep	to,response
R1319	T9372	T9371	pobj	hypovolemia,to
R1320	T9373	T9372	cc	or,hypovolemia
R1321	T9374	T9372	conj	hypernatremia,hypovolemia
R1322	T9375	T9376	punct	[,21
R1323	T9418	T9420	compound	type,mice
R1324	T9376	T9370	parataxis	21,response
R1325	T9377	T9376	punct	],21
R1326	T9378	T9367	punct	", ",secretes
R1327	T9379	T9367	dobj	AVP,secretes
R1328	T9419	T9418	punct	-,type
R1329	T9380	T9355	punct	.,is
R1330	T9382	T9383	det	The,analog
R1331	T9420	T9416	pobj	mice,to
R1332	T9383	T9386	nsubj	analog,is
R1333	T9384	T9383	amod	synthetic,analog
R1334	T9421	T9415	punct	", ",leads
R1335	T9385	T9383	compound	AVP,analog
R1336	T9387	T9383	punct	", ",analog
R1337	T9422	T9415	nsubj	dDAVP,leads
R1338	T9388	T9389	nummod	1,deamino
R1339	T9389	T9391	nmod	deamino,arginine
R1340	T9390	T9389	punct	-,deamino
R1341	T9423	T9415	prep	to,leads
R1342	T9424	T9425	det	a,increase
R1343	T9391	T9397	compound	arginine,vasopressin
R1344	T9425	T9423	pobj	increase,to
R1345	T9392	T9391	punct	-,arginine
R1346	T9393	T9391	nummod	8,arginine
R1347	T9426	T9425	amod	dramatic,increase
R1348	T9394	T9391	punct	-,arginine
R1349	T9395	T9391	compound	D,arginine
R1350	T9396	T9391	punct	-,arginine
R1351	T9427	T9425	prep	in,increase
R1352	T9397	T9383	appos	vasopressin,analog
R1353	T9398	T9397	punct	(,vasopressin
R1354	T9399	T9397	appos	dDAVP,vasopressin
R1355	T9428	T9429	compound	urine,concentration
R1356	T9400	T9397	punct	;,vasopressin
R1357	T9401	T9402	advmod	also,called
R1358	T9402	T9397	acl	called,vasopressin
R1359	T9429	T9427	pobj	concentration,in
R1360	T9430	T9425	punct	", ",increase
R1361	T9431	T9425	prep	from,increase
R1362	T9432	T9431	pobj	"1,293",from
R1363	T9509	T9510	dep	which,be
R1364	T9510	T9505	relcl	be,channel
R1365	T9433	T9431	prep	to,from
R1366	T9511	T9510	aux	must,be
R1367	T9512	T9510	acomp	sufficient,be
R1368	T9513	T9514	aux	to,allow
R1369	T9434	T9433	pobj	"5,885",to
R1370	T9514	T9512	xcomp	allow,sufficient
R1371	T9515	T9514	dobj	survival,allow
R1372	T9516	T9515	prep	of,survival
R1373	T9517	T9518	det	the,individual
R1374	T9518	T9516	pobj	individual,of
R1375	T9519	T9497	punct	", ",indicates
R1376	T9435	T9431	pobj	mOsm,from
R1377	T9520	T9497	prep	in,indicates
R1378	T9521	T9520	pobj	contrast,in
R1379	T9522	T9521	prep	to,contrast
R1380	T9436	T9437	punct	(,Figure
R1381	T9523	T9524	det	the,mouse
R1382	T9524	T9522	pobj	mouse,to
R1383	T9437	T9415	parataxis	Figure,leads
R1384	T9525	T9524	nmod	T126M,mouse
R1385	T9526	T9524	amod	knock,mouse
R1386	T9527	T9526	punct	-,knock
R1387	T9528	T9526	prt	in,knock
R1388	T9438	T9437	advmod	4.6-fold,Figure
R1389	T9529	T9530	punct	[,18
R1390	T9530	T9497	parataxis	18,indicates
R1391	T9531	T9530	punct	],18
R1392	T9439	T9437	punct	;,Figure
R1393	T9532	T9497	punct	.,indicates
R1394	T9534	T9535	amod	Multiple,matings
R1395	T9440	T9437	nummod	1D,Figure
R1396	T9535	T9537	nsubj	matings,yielded
R1397	T9536	T9535	amod	heterozygous,matings
R1398	T9441	T9437	punct	),Figure
R1399	T9538	T9539	nummod	101,animals
R1400	T9539	T9537	dobj	animals,yielded
R1401	T9442	T9415	punct	.,leads
R1402	T9540	T9539	punct	", ",animals
R1403	T9541	T9542	dep	which,appeared
R1404	T9542	T9539	relcl	appeared,animals
R1405	T9543	T9542	prep	at,appeared
R1406	T9444	T9445	prep	With,concentrate
R1407	T9544	T9545	det	a,ratio
R1408	T9545	T9543	pobj	ratio,at
R1409	T9546	T9545	prep	of,ratio
R1410	T9547	T9546	pobj	26,of
R1411	T9446	T9447	amod	similar,treatment
R1412	T9548	T9549	punct	:,49
R1413	T9549	T9547	prep	49,26
R1414	T9550	T9551	punct	:,26
R1415	T9447	T9444	pobj	treatment,With
R1416	T9551	T9547	prep	26,26
R1417	T9552	T9542	punct	", ",appeared
R1418	T9553	T9542	prep	near,appeared
R1419	T9448	T9445	punct	", ",concentrate
R1420	T9554	T9555	det	the,frequencies
R1421	T9555	T9553	pobj	frequencies,near
R1422	T9556	T9555	amod	expected,frequencies
R1423	T9557	T9558	amod	Mendelian,type
R1424	T9558	T9555	nmod	type,frequencies
R1425	T9559	T9558	amod	wild,type
R1426	T9449	T9450	compound	mutant,mice
R1427	T9560	T9558	punct	", ",type
R1428	T9561	T9558	conj	heterozygote,type
R1429	T9562	T9561	punct	", ",heterozygote
R1430	T9450	T9445	nsubj	mice,concentrate
R1431	T9563	T9561	cc	and,heterozygote
R1432	T9564	T9561	conj	mutant,heterozygote
R1433	T9565	T9542	punct	", ",appeared
R1434	T9451	T9452	poss	their,urine
R1435	T9566	T9542	advmod	respectively,appeared
R1436	T9567	T9537	punct	", ",yielded
R1437	T9452	T9445	dobj	urine,concentrate
R1438	T9568	T9537	advcl	indicating,yielded
R1439	T9569	T9570	mark	that,is
R1440	T9570	T9568	ccomp	is,indicating
R1441	T9571	T9570	expl	there,is
R1442	T9453	T9445	prep	to,concentrate
R1443	T9572	T9573	det	no,viability
R1444	T9573	T9570	attr	viability,is
R1445	T9454	T9455	det	a,extent
R1446	T9574	T9573	amod	reduced,viability
R1447	T9575	T9573	acl	associated,viability
R1448	T9576	T9575	prep	with,associated
R1449	T9455	T9453	pobj	extent,to
R1450	T9577	T9578	det	this,mutation
R1451	T9578	T9576	pobj	mutation,with
R1452	T9579	T9537	punct	.,yielded
R1453	T9456	T9455	amod	lesser,extent
R1454	T9581	T9582	amod	Other,was
R1455	T9457	T9456	cc	but,lesser
R1456	T9583	T9581	prep	than,Other
R1457	T9458	T9459	advmod	still,significant
R1458	T9459	T9456	conj	significant,lesser
R1459	T9584	T9585	det	the,production
R1460	T9585	T9583	pobj	production,than
R1461	T9586	T9585	amod	increased,production
R1462	T9460	T9445	punct	", ",concentrate
R1463	T9587	T9585	compound	urine,production
R1464	T9588	T9585	cc	and,production
R1465	T9589	T9590	compound	water,intake
R1466	T9461	T9445	prep	from,concentrate
R1467	T9590	T9585	conj	intake,production
R1468	T9591	T9582	punct	", ",was
R1469	T9592	T9582	expl	there,was
R1470	T9462	T9461	pobj	161,from
R1471	T9593	T9594	det	no,phenotype
R1472	T9594	T9582	attr	phenotype,was
R1473	T9463	T9461	prep	to,from
R1474	T9595	T9594	amod	overt,phenotype
R1475	T9596	T9594	prep	in,phenotype
R1476	T9597	T9598	compound	mutant,mice
R1477	T9598	T9596	pobj	mice,in
R1478	T9599	T9594	punct	", ",phenotype
R1479	T9600	T9594	prep	save,phenotype
R1480	T9464	T9463	pobj	470,to
R1481	T9601	T9602	amod	distended,kidneys
R1482	T9602	T9600	pobj	kidneys,save
R1483	T9603	T9602	punct	", ",kidneys
R1484	T9465	T9461	pobj	mOsm,from
R1485	T9604	T9605	dep	which,appeared
R1486	T9605	T9602	relcl	appeared,kidneys
R1487	T9606	T9605	advmod	variably,appeared
R1488	T9466	T9467	punct	(,2.9-fold
R1489	T9607	T9605	prep	in,appeared
R1490	T9608	T9609	amod	adult,animals
R1491	T9609	T9607	pobj	animals,in
R1492	T9467	T9461	parataxis	2.9-fold,from
R1493	T9610	T9611	punct	(,Figure
R1494	T9611	T9582	parataxis	Figure,was
R1495	T9612	T9611	nummod	2A,Figure
R1496	T9468	T9467	punct	),2.9-fold
R1497	T9613	T9611	punct	),Figure
R1498	T9614	T9582	punct	.,was
R1499	T9469	T9445	punct	", ",concentrate
R1500	T9470	T9445	advcl	indicating,concentrate
R1501	T9471	T9472	mark	that,are
R1502	T9616	T9617	mark	Although,measured
R1503	T9472	T9470	ccomp	are,indicating
R1504	T9617	T9620	advcl	measured,seem
R1505	T9618	T9617	neg	not,measured
R1506	T9619	T9617	advmod	specifically,measured
R1507	T9473	T9474	det	these,animals
R1508	T9621	T9620	punct	", ",seem
R1509	T9622	T9623	compound	mutant,mice
R1510	T9474	T9472	nsubj	animals,are
R1511	T9623	T9620	nsubj	mice,seem
R1512	T9624	T9625	aux	to,have
R1513	T9625	T9620	xcomp	have,seem
R1514	T9475	T9472	preconj	not,are
R1515	T9626	T9627	det	a,lifespan
R1516	T9627	T9625	dobj	lifespan,have
R1517	T9628	T9627	amod	normal,lifespan
R1518	T9476	T9475	advmod	only,not
R1519	T9629	T9620	punct	.,seem
R1520	T9477	T9472	conj	unable,are
R1521	T9631	T9632	det	The,animal
R1522	T9632	T9634	nsubj	animal,lived
R1523	T9633	T9632	nummod	one,animal
R1524	T9478	T9479	aux	to,concentrate
R1525	T9635	T9636	dep	that,followed
R1526	T9636	T9632	relcl	followed,animal
R1527	T9637	T9636	auxpass	was,followed
R1528	T9638	T9634	prep	to,lived
R1529	T9639	T9640	nummod	18,mo
R1530	T9479	T9477	xcomp	concentrate,unable
R1531	T9640	T9638	pobj	mo,to
R1532	T9641	T9634	punct	", ",lived
R1533	T9642	T9634	advcl	typical,lived
R1534	T9480	T9481	poss	their,urine
R1535	T9643	T9642	prep	for,typical
R1536	T9644	T9643	pobj	animals,for
R1537	T9645	T9644	prep	in,animals
R1538	T9481	T9479	dobj	urine,concentrate
R1539	T9646	T9647	poss	our,colony
R1540	T9647	T9645	pobj	colony,in
R1541	T9648	T9634	punct	.,lived
R1542	T9482	T9479	advmod	properly,concentrate
R1543	T9650	T9651	compound	Aqp2F204V,F204V
R1544	T9651	T9653	compound	F204V,mice
R1545	T9483	T9472	cc	but,are
R1546	T9652	T9651	punct	/,F204V
R1547	T9653	T9654	nsubj	mice,suffer
R1548	T9484	T9472	conj	are,are
R1549	T9655	T9654	prep	from,suffer
R1550	T9656	T9657	amod	severe,hydronephrosis
R1551	T9485	T9484	advmod	also,are
R1552	T9657	T9655	pobj	hydronephrosis,from
R1553	T9658	T9659	punct	(,2A
R1554	T9486	T9484	acomp	defective,are
R1555	T9659	T9654	parataxis	2A,suffer
R1556	T9660	T9659	nmod	Figure,2A
R1557	T9661	T9659	cc	and,2A
R1558	T9487	T9484	prep	in,are
R1559	T9662	T9659	conj	2B,2A
R1560	T9663	T9659	punct	),2A
R1561	T9664	T9654	punct	", ",suffer
R1562	T9488	T9489	poss	their,response
R1563	T9665	T9666	advmod	presumably,as
R1564	T9666	T9654	prep	as,suffer
R1565	T9667	T9668	det	a,consequence
R1566	T9489	T9487	pobj	response,in
R1567	T9668	T9666	pobj	consequence,as
R1568	T9669	T9668	prep	of,consequence
R1569	T9490	T9489	prep	to,response
R1570	T9491	T9490	pobj	dDAVP,to
R1571	T9670	T9671	det	an,inability
R1572	T9492	T9445	punct	.,concentrate
R1573	T9671	T9669	pobj	inability,of
R1574	T9672	T9673	aux	to,cope
R1575	T9673	T9671	acl	cope,inability
R1576	T9494	T9495	det	The,response
R1577	T9674	T9673	prep	with,cope
R1578	T9675	T9676	det	the,polyuria
R1579	T9676	T9674	pobj	polyuria,with
R1580	T9677	T9676	amod	extreme,polyuria
R1581	T9678	T9654	punct	.,suffer
R1582	T9495	T9497	nsubj	response,indicates
R1583	T9680	T9681	nsubj	We,found
R1584	T9681	T9682	ccomp	found,was
R1585	T9496	T9495	amod	smaller,response
R1586	T9683	T9684	amod	distended,kidneys
R1587	T9684	T9681	dobj	kidneys,found
R1588	T9685	T9681	prep	in,found
R1589	T9498	T9495	prep	to,response
R1590	T9686	T9687	det	all,mice
R1591	T9687	T9685	pobj	mice,in
R1592	T9688	T9689	compound	Aqp2F204V,F204V
R1593	T9689	T9687	compound	F204V,mice
R1594	T9499	T9498	pobj	dDAVP,to
R1595	T9690	T9689	punct	/,F204V
R1596	T9691	T9682	punct	;,was
R1597	T9692	T9682	advmod	however,was
R1598	T9500	T9501	det	some,activity
R1599	T9693	T9682	punct	", ",was
R1600	T9694	T9695	det	the,degree
R1601	T9695	T9682	nsubj	degree,was
R1602	T9501	T9497	dobj	activity,indicates
R1603	T9696	T9695	prep	of,degree
R1604	T9697	T9696	pobj	inflation,of
R1605	T9502	T9501	amod	residual,activity
R1606	T9698	T9682	acomp	variable,was
R1607	T9699	T9682	prep	in,was
R1608	T9700	T9701	amod	affected,mice
R1609	T9503	T9501	prep	of,activity
R1610	T9701	T9699	pobj	mice,in
R1611	T9702	T9682	cc	and,was
R1612	T9703	T9682	conj	worsened,was
R1613	T9704	T9703	prep	with,worsened
R1614	T9504	T9505	det	the,channel
R1615	T9705	T9704	pobj	age,with
R1616	T9706	T9682	punct	.,was
R1617	T9505	T9503	pobj	channel,of
R1618	T9708	T9709	amod	Severe,hydronephrosis
R1619	T9709	T9710	nsubjpass	hydronephrosis,observed
R1620	T9506	T9505	compound	mutant,channel
R1621	T9711	T9710	aux	has,observed
R1622	T9712	T9710	advmod	previously,observed
R1623	T9507	T9505	compound	AQP2,channel
R1624	T9713	T9710	auxpass	been,observed
R1625	T9714	T9710	prep	in,observed
R1626	T9715	T9716	amod	double,knock
R1627	T9508	T9505	punct	", ",channel
R1628	T9716	T9720	amod	knock,mice
R1629	T9717	T9718	compound	Aqp1,Aqp3
R1630	T9718	T9716	npadvmod	Aqp3,knock
R1631	T9719	T9718	punct	/,Aqp3
R1632	T9720	T9714	pobj	mice,in
R1633	T9827	T9828	det	the,features
R1634	T9721	T9716	punct	-,knock
R1635	T9828	T9825	nsubj	features,were
R1636	T9722	T9716	prt	out,knock
R1637	T9723	T9724	punct	[,17
R1638	T9829	T9828	amod	morphologic,features
R1639	T9724	T9710	parataxis	17,observed
R1640	T9725	T9724	punct	],17
R1641	T9726	T9710	punct	", ",observed
R1642	T9830	T9828	prep	of,features
R1643	T9727	T9710	cc	and,observed
R1644	T9728	T9710	conj	appears,observed
R1645	T9729	T9728	prep	at,appears
R1646	T9831	T9832	det	the,glomeruli
R1647	T9730	T9731	nummod	6,wk
R1648	T9731	T9729	pobj	wk,at
R1649	T9732	T9710	punct	.,observed
R1650	T9832	T9830	pobj	glomeruli,of
R1651	T9734	T9735	advmod	Even,at
R1652	T9833	T9832	cc	and,glomeruli
R1653	T9735	T9736	prep	at,had
R1654	T9737	T9738	nummod	4,wk
R1655	T9834	T9835	amod	proximal,distal
R1656	T9738	T9735	pobj	wk,at
R1657	T9739	T9736	punct	", ",had
R1658	T9835	T9837	amod	distal,tubules
R1659	T9740	T9741	compound	Aqp2F204V,F204V
R1660	T9741	T9743	compound	F204V,mice
R1661	T9742	T9741	punct	/,F204V
R1662	T9743	T9736	nsubj	mice,had
R1663	T9836	T9835	punct	/,distal
R1664	T9744	T9736	dobj	hydronephrosis,had
R1665	T9745	T9736	punct	.,had
R1666	T9837	T9832	conj	tubules,glomeruli
R1667	T9747	T9748	amod	Histologic,sections
R1668	T9748	T9749	nsubj	sections,demonstrated
R1669	T9838	T9825	acomp	unremarkable,were
R1670	T9750	T9748	prep	from,sections
R1671	T9751	T9752	compound	Aqp2F204V,F204V
R1672	T9839	T9840	punct	(,Figure
R1673	T9752	T9754	compound	F204V,mice
R1674	T9753	T9752	punct	/,F204V
R1675	T9754	T9750	pobj	mice,from
R1676	T9840	T9825	parataxis	Figure,were
R1677	T9755	T9756	amod	marked,dilatation
R1678	T9756	T9749	dobj	dilatation,demonstrated
R1679	T9841	T9840	nummod	2D,Figure
R1680	T9757	T9756	prep	of,dilatation
R1681	T9758	T9759	det	the,pelvis
R1682	T9842	T9840	punct	),Figure
R1683	T9759	T9757	pobj	pelvis,of
R1684	T9760	T9759	amod	renal,pelvis
R1685	T9761	T9756	cc	yet,dilatation
R1686	T9762	T9763	amod	normal,morphology
R1687	T9843	T9825	punct	.,were
R1688	T9763	T9756	conj	morphology,dilatation
R1689	T9764	T9763	prep	of,morphology
R1690	T9765	T9766	det	the,ureter
R1691	T9845	T9846	mark	As,shown
R1692	T9766	T9764	pobj	ureter,of
R1693	T9767	T9768	punct	(,2C
R1694	T9768	T9749	parataxis	2C,demonstrated
R1695	T9846	T9847	advcl	shown,revealed
R1696	T9769	T9768	nmod	Figure,2C
R1697	T9770	T9768	cc	and,2C
R1698	T9771	T9768	conj	2D,2C
R1699	T9848	T9846	advmod	previously,shown
R1700	T9772	T9768	punct	),2C
R1701	T9773	T9749	punct	.,demonstrated
R1702	T9849	T9850	punct	[,22
R1703	T9775	T9776	prep	In,was
R1704	T9777	T9775	amod	particular,In
R1705	T9778	T9776	punct	", ",was
R1706	T9779	T9780	det	the,propria
R1707	T9850	T9846	parataxis	22,shown
R1708	T9780	T9776	nsubj	propria,was
R1709	T9781	T9780	compound	muscularis,propria
R1710	T9782	T9783	preconj	neither,hypertrophied
R1711	T9851	T9850	nummod	18,22
R1712	T9852	T9850	punct	",",22
R1713	T9783	T9776	acomp	hypertrophied,was
R1714	T9853	T9850	punct	],22
R1715	T9784	T9783	cc	nor,hypertrophied
R1716	T9785	T9783	conj	thinned,hypertrophied
R1717	T9854	T9847	punct	", ",revealed
R1718	T9786	T9776	punct	.,was
R1719	T9855	T9847	nsubj	immunoblotting,revealed
R1720	T9788	T9789	expl	There,was
R1721	T9790	T9791	det	the,appearance
R1722	T9856	T9857	nummod	three,forms
R1723	T9791	T9789	attr	appearance,was
R1724	T9792	T9791	amod	normal,appearance
R1725	T9857	T9847	dobj	forms,revealed
R1726	T9793	T9791	amod	festooned,appearance
R1727	T9794	T9791	prep	of,appearance
R1728	T9795	T9796	det	the,urothelium
R1729	T9796	T9794	pobj	urothelium,of
R1730	T9858	T9857	amod	different,forms
R1731	T9797	T9789	punct	", ",was
R1732	T9798	T9789	cc	and,was
R1733	T9859	T9857	prep	of,forms
R1734	T9799	T9800	det	this,epithelium
R1735	T9800	T9802	nsubj	epithelium,was
R1736	T9801	T9800	amod	transitional,epithelium
R1737	T9802	T9789	conj	was,was
R1738	T9803	T9802	prep	of,was
R1739	T9804	T9805	amod	normal,thickness
R1740	T9860	T9859	pobj	AQP2,of
R1741	T9805	T9803	pobj	thickness,of
R1742	T9806	T9802	punct	.,was
R1743	T9861	T9847	punct	", ",revealed
R1744	T9808	T9809	expl	There,was
R1745	T9810	T9809	attr	thinning,was
R1746	T9862	T9847	prep	due,revealed
R1747	T9811	T9810	prep	of,thinning
R1748	T9812	T9813	det	the,kidney
R1749	T9813	T9811	pobj	kidney,of
R1750	T9863	T9862	pcomp	to,due
R1751	T9814	T9815	mark	as,measured
R1752	T9815	T9809	advcl	measured,was
R1753	T9816	T9815	prep	from,measured
R1754	T9864	T9865	amod	different,degrees
R1755	T9817	T9818	amod	renal,capsule
R1756	T9818	T9816	pobj	capsule,from
R1757	T9819	T9816	prep	to,from
R1758	T9865	T9862	pobj	degrees,due
R1759	T9820	T9821	amod	renal,pelvis
R1760	T9821	T9819	pobj	pelvis,to
R1761	T9866	T9865	cc	and,degrees
R1762	T9822	T9809	punct	.,was
R1763	T9824	T9825	advmod	However,were
R1764	T9867	T9865	conj	forms,degrees
R1765	T9826	T9825	punct	", ",were
R1766	T9868	T9865	prep	of,degrees
R1767	T9869	T9868	pobj	glycosylation,of
R1768	T9870	T9871	punct	(,Figure
R1769	T9871	T9847	parataxis	Figure,revealed
R1770	T9872	T9871	nummod	3A,Figure
R1771	T9933	T9932	punct	],22
R1772	T9873	T9871	punct	),Figure
R1773	T9934	T9911	punct	.,is
R1774	T9874	T9847	punct	.,revealed
R1775	T9936	T9937	prep	Compared,reduced
R1776	T9876	T9877	amod	Previous,reports
R1777	T9938	T9936	prep	to,Compared
R1778	T9939	T9938	pobj	that,to
R1779	T9940	T9939	prep	from,that
R1780	T9941	T9942	det	the,kidneys
R1781	T9877	T9878	nsubj	reports,demonstrated
R1782	T9942	T9940	pobj	kidneys,from
R1783	T9943	T9942	prep	of,kidneys
R1784	T9879	T9878	aux	have,demonstrated
R1785	T9944	T9945	amod	wild,type
R1786	T9945	T9947	compound	type,animals
R1787	T9946	T9945	punct	-,type
R1788	T9947	T9943	pobj	animals,of
R1789	T9948	T9937	punct	", ",reduced
R1790	T9949	T9937	nsubjpass	AQP2,reduced
R1791	T9880	T9881	mark	that,appears
R1792	T9950	T9949	prep	from,AQP2
R1793	T9951	T9952	compound	mutant,animals
R1794	T9952	T9950	pobj	animals,from
R1795	T9881	T9878	ccomp	appears,demonstrated
R1796	T9953	T9937	auxpass	was,reduced
R1797	T9954	T9937	prep	in,reduced
R1798	T9955	T9956	preconj	both,form
R1799	T9882	T9883	amod	nonglycosylated,protein
R1800	T9956	T9954	pobj	form,in
R1801	T9957	T9956	det	the,form
R1802	T9883	T9881	nsubj	protein,appears
R1803	T9958	T9959	amod	high,weight
R1804	T9959	T9956	nmod	weight,form
R1805	T9960	T9959	amod	molecular,weight
R1806	T9884	T9881	prep	as,appears
R1807	T9961	T9956	punct	", ",form
R1808	T9962	T9956	amod	diffuse,form
R1809	T9963	T9956	cc	and,form
R1810	T9885	T9886	det	a,band
R1811	T9964	T9965	det	the,form
R1812	T9965	T9956	conj	form,form
R1813	T9966	T9967	amod	lowest,weight
R1814	T9886	T9884	pobj	band,as
R1815	T9967	T9965	compound	weight,form
R1816	T9968	T9967	amod	molecular,weight
R1817	T9969	T9937	punct	", ",reduced
R1818	T9887	T9888	nummod	29,kDa
R1819	T9970	T9937	cc	but,reduced
R1820	T9971	T9937	conj	enriched,reduced
R1821	T9888	T9886	compound	kDa,band
R1822	T9972	T9971	prep	in,enriched
R1823	T9973	T9974	det	the,form
R1824	T9974	T9972	pobj	form,in
R1825	T9889	T9881	punct	", ",appears
R1826	T9975	T9976	amod	intermediate,weight
R1827	T9976	T9974	compound	weight,form
R1828	T9977	T9976	amod	molecular,weight
R1829	T9890	T9891	mark	while,runs
R1830	T9978	T9979	punct	(,Figure
R1831	T9979	T9971	parataxis	Figure,enriched
R1832	T9980	T9979	nummod	3A,Figure
R1833	T9891	T9881	advcl	runs,appears
R1834	T9981	T9979	punct	),Figure
R1835	T9982	T9937	punct	.,reduced
R1836	T9892	T9893	amod	complex,protein
R1837	T9984	T9985	amod	Heterozygous,animals
R1838	T9985	T9986	nsubj	animals,showed
R1839	T9987	T9988	amod	intermediate,amounts
R1840	T9988	T9986	dobj	amounts,showed
R1841	T9893	T9891	nsubj	protein,runs
R1842	T9989	T9988	prep	of,amounts
R1843	T9990	T9991	det	all,forms
R1844	T9991	T9989	pobj	forms,of
R1845	T9894	T9893	amod	glycosylated,protein
R1846	T9992	T9991	nummod	three,forms
R1847	T9993	T9986	punct	.,showed
R1848	T9895	T9891	prep	as,runs
R1849	T9995	T9996	det	The,nature
R1850	T9996	T9997	nsubjpass	nature,revealed
R1851	T9896	T9897	det	a,smear
R1852	T9998	T9996	prep	of,nature
R1853	T9999	T10000	det	these,forms
R1854	T9897	T9895	pobj	smear,as
R1855	T10000	T9998	pobj	forms,of
R1856	T10001	T10000	amod	glycosylated,forms
R1857	T9898	T9897	prep	between,smear
R1858	T10002	T9997	auxpass	was,revealed
R1859	T10003	T9997	prep	by,revealed
R1860	T10004	T10003	pobj	digestion,by
R1861	T9899	T9900	nummod	35,kDa
R1862	T10005	T10004	prep	with,digestion
R1863	T10006	T10007	compound	endoglycosidase,H
R1864	T10007	T10005	pobj	H,with
R1865	T9900	T9898	pobj	kDa,between
R1866	T10008	T10007	punct	", ",H
R1867	T10009	T10010	dep	which,cleaves
R1868	T9901	T9899	cc	and,35
R1869	T10010	T10007	relcl	cleaves,H
R1870	T10011	T10010	advmod	specifically,cleaves
R1871	T10012	T10013	npadvmod	mannose,rich
R1872	T9902	T9899	conj	45,35
R1873	T10013	T10015	amod	rich,carbohydrate
R1874	T10014	T10013	punct	-,rich
R1875	T10015	T10010	dobj	carbohydrate,cleaves
R1876	T9903	T9878	punct	.,demonstrated
R1877	T10016	T10010	prep	from,cleaves
R1878	T10017	T10018	det	the,backbone
R1879	T10018	T10016	pobj	backbone,from
R1880	T9905	T9906	det	A,form
R1881	T10019	T10018	compound	protein,backbone
R1882	T9906	T9911	nsubj	form,is
R1883	T10020	T9997	punct	.,revealed
R1884	T9907	T9908	amod	short,lived
R1885	T10022	T10023	nsubj	Treatment,affected
R1886	T9908	T9906	amod	lived,form
R1887	T10024	T10022	prep	of,Treatment
R1888	T10025	T10026	amod	endogenous,AQP2
R1889	T10026	T10024	pobj	AQP2,of
R1890	T9909	T9908	punct	-,lived
R1891	T10027	T10026	prep	from,AQP2
R1892	T10028	T10027	pobj	kidneys,from
R1893	T10029	T10028	prep	of,kidneys
R1894	T10030	T10031	amod	wild,type
R1895	T10031	T10033	nmod	type,animals
R1896	T9910	T9906	amod	intermediate,form
R1897	T10032	T10031	punct	-,type
R1898	T10033	T10029	pobj	animals,of
R1899	T9912	T9906	prep	of,form
R1900	T10034	T10031	punct	", ",type
R1901	T10035	T10031	conj	heterozygous,type
R1902	T10036	T10035	punct	", ",heterozygous
R1903	T10037	T10035	cc	and,heterozygous
R1904	T9913	T9914	nummod	31,kDa
R1905	T10038	T10035	conj	mutant,heterozygous
R1906	T9914	T9912	pobj	kDa,of
R1907	T9915	T9906	acl	representing,form
R1908	T9916	T9917	nmod	core,glycosylation
R1909	T10039	T10023	advmod	specifically,affected
R1910	T10040	T10041	det	the,form
R1911	T9917	T9915	dobj	glycosylation,representing
R1912	T10041	T10023	dobj	form,affected
R1913	T10042	T10043	amod	intermediate,weight
R1914	T10043	T10041	compound	weight,form
R1915	T9918	T9917	punct	", ",glycosylation
R1916	T10044	T10043	amod	molecular,weight
R1917	T10045	T10046	punct	(,Figure
R1918	T9919	T9920	amod	high,mannose
R1919	T10046	T10023	parataxis	Figure,affected
R1920	T10047	T10046	nummod	3B,Figure
R1921	T10048	T10046	punct	),Figure
R1922	T9920	T9917	compound	mannose,glycosylation
R1923	T10049	T10023	punct	.,affected
R1924	T10051	T10052	det	The,presence
R1925	T9921	T9920	punct	-,mannose
R1926	T10052	T10053	nsubj	presence,permits
R1927	T9922	T9917	prep	of,glycosylation
R1928	T10054	T10052	prep	of,presence
R1929	T10055	T10056	det	some,proteins
R1930	T10056	T10054	pobj	proteins,of
R1931	T9923	T9922	pobj	AQP2,of
R1932	T10057	T10056	amod	mature,proteins
R1933	T10058	T10056	amod	glycosylated,proteins
R1934	T10059	T10060	punct	(,kDa
R1935	T9924	T9911	acomp	apparent,is
R1936	T10060	T10056	parataxis	kDa,proteins
R1937	T10061	T10062	quantmod	35,45
R1938	T10062	T10060	nummod	45,kDa
R1939	T9925	T9924	prep	from,apparent
R1940	T10063	T10062	punct	–,45
R1941	T10064	T10060	punct	),kDa
R1942	T9926	T9927	compound	pulse,chase
R1943	T10065	T10056	prep	in,proteins
R1944	T10066	T10067	compound	Aqp2F204V,F204V
R1945	T10067	T10069	compound	F204V,mice
R1946	T10068	T10067	punct	/,F204V
R1947	T10069	T10065	pobj	mice,in
R1948	T10070	T10053	advmod	presumably,permits
R1949	T9927	T9929	compound	chase,labeling
R1950	T10071	T10072	poss	their,survival
R1951	T10072	T10053	dobj	survival,permits
R1952	T10073	T10072	prep	compared,survival
R1953	T9928	T9927	punct	-,chase
R1954	T10074	T10073	prep	to,compared
R1955	T10075	T10076	compound	Aqp2T126M,T126M
R1956	T10076	T10078	compound	T126M,mice
R1957	T9929	T9930	compound	labeling,experiments
R1958	T10077	T10076	punct	/,T126M
R1959	T10078	T10074	pobj	mice,to
R1960	T10079	T10053	punct	", ",permits
R1961	T9930	T9925	pobj	experiments,from
R1962	T10080	T10053	cc	and,permits
R1963	T10081	T10053	conj	is,permits
R1964	T10082	T10081	acomp	consistent,is
R1965	T9931	T9932	punct	[,22
R1966	T10083	T10082	prep	with,consistent
R1967	T10084	T10085	det	a,response
R1968	T9932	T9911	parataxis	22,is
R1969	T10085	T10083	pobj	response,with
R1970	T10086	T10085	amod	diminished,response
R1971	T10087	T10085	prep	to,response
R1972	T10088	T10087	pobj	dDAVP,to
R1973	T10146	T10147	prep	In,fail
R1974	T10089	T10053	punct	.,permits
R1975	T10091	T10092	prep	In,postulated
R1976	T10093	T10091	pobj	humans,In
R1977	T10148	T10146	amod	general,In
R1978	T10094	T10092	punct	", ",postulated
R1979	T10095	T10096	amod	recessive,alleles
R1980	T10096	T10092	nsubjpass	alleles,postulated
R1981	T10097	T10096	prep	of,alleles
R1982	T10149	T10147	punct	", ",fail
R1983	T10098	T10097	pobj	Aqp2,of
R1984	T10099	T10092	auxpass	are,postulated
R1985	T10100	T10101	aux	to,cause
R1986	T10150	T10151	amod	such,alleles
R1987	T10101	T10092	xcomp	cause,postulated
R1988	T10102	T10101	dobj	NDI,cause
R1989	T10103	T10104	mark	because,translocate
R1990	T10151	T10147	nsubj	alleles,fail
R1991	T10152	T10151	amod	recessive,alleles
R1992	T10104	T10101	advcl	translocate,cause
R1993	T10105	T10104	nsubj	they,translocate
R1994	T10153	T10147	punct	", ",fail
R1995	T10106	T10104	aux	do,translocate
R1996	T10107	T10104	neg	not,translocate
R1997	T10108	T10104	advmod	properly,translocate
R1998	T10109	T10104	prep	to,translocate
R1999	T10110	T10111	det	the,surface
R2000	T10154	T10155	advmod	when,visualized
R2001	T10111	T10109	pobj	surface,to
R2002	T10112	T10111	amod	apical,surface
R2003	T10113	T10111	compound	cell,surface
R2004	T10114	T10104	prep	in,translocate
R2005	T10155	T10147	advcl	visualized,fail
R2006	T10115	T10114	pobj	response,in
R2007	T10116	T10115	prep	to,response
R2008	T10156	T10155	advmod	immunocytochemically,visualized
R2009	T10117	T10116	pobj	AVP,to
R2010	T10118	T10092	punct	.,postulated
R2011	T10157	T10147	punct	", ",fail
R2012	T10120	T10121	det	This,postulate
R2013	T10121	T10122	nsubj	postulate,comes
R2014	T10158	T10159	aux	to,localize
R2015	T10123	T10124	advmod	solely,from
R2016	T10124	T10122	prep	from,comes
R2017	T10159	T10147	xcomp	localize,fail
R2018	T10125	T10126	advmod	in,vitro
R2019	T10126	T10127	amod	vitro,studies
R2020	T10127	T10124	pobj	studies,from
R2021	T10160	T10159	prep	to,localize
R2022	T10128	T10129	prep	in,transfected
R2023	T10129	T10127	relcl	transfected,studies
R2024	T10130	T10128	pobj	which,in
R2025	T10161	T10162	npadvmod	AVP,responsive
R2026	T10131	T10132	compound	mutant,cDNAs
R2027	T10132	T10129	nsubjpass	cDNAs,transfected
R2028	T10133	T10132	compound	Aqp2,cDNAs
R2029	T10162	T10164	amod	responsive,vesicles
R2030	T10134	T10132	acl	corresponding,cDNAs
R2031	T10135	T10134	prep	to,corresponding
R2032	T10136	T10137	amod	human,mutations
R2033	T10163	T10162	punct	-,responsive
R2034	T10137	T10135	pobj	mutations,to
R2035	T10138	T10137	compound	disease,mutations
R2036	T10164	T10160	pobj	vesicles,to
R2037	T10139	T10129	auxpass	are,transfected
R2038	T10140	T10129	prep	into,transfected
R2039	T10141	T10142	compound	kidney,lines
R2040	T10165	T10147	punct	.,fail
R2041	T10142	T10140	pobj	lines,into
R2042	T10143	T10142	compound	cell,lines
R2043	T10144	T10122	punct	.,comes
R2044	T10167	T10168	advmod	Rather,trapped
R2045	T10169	T10168	punct	", ",trapped
R2046	T10170	T10168	nsubjpass	they,trapped
R2047	T10171	T10168	auxpass	get,trapped
R2048	T10172	T10168	prep	in,trapped
R2049	T10250	T10237	parataxis	row,translocated
R2050	T10251	T10250	dep	Figure,row
R2051	T10173	T10174	det	the,reticulum
R2052	T10252	T10251	nummod	4A,Figure
R2053	T10253	T10250	punct	", ",row
R2054	T10174	T10172	pobj	reticulum,in
R2055	T10254	T10250	amod	second,row
R2056	T10255	T10250	punct	),row
R2057	T10256	T10237	punct	.,translocated
R2058	T10175	T10174	amod	endoplasmic,reticulum
R2059	T10258	T10259	prep	In,distributed
R2060	T10176	T10174	punct	(,reticulum
R2061	T10260	T10258	pobj	kidneys,In
R2062	T10261	T10260	acl	taken,kidneys
R2063	T10177	T10174	appos	ER,reticulum
R2064	T10262	T10261	prep	from,taken
R2065	T10263	T10264	compound	mutant,animals
R2066	T10264	T10262	pobj	animals,from
R2067	T10178	T10168	punct	),trapped
R2068	T10265	T10259	punct	", ",distributed
R2069	T10266	T10259	advmod	however,distributed
R2070	T10267	T10259	punct	", ",distributed
R2071	T10179	T10168	punct	.,trapped
R2072	T10268	T10259	nsubjpass	AQP2,distributed
R2073	T10269	T10259	auxpass	was,distributed
R2074	T10270	T10259	advmod	randomly,distributed
R2075	T10271	T10259	prep	throughout,distributed
R2076	T10181	T10182	poss	Our,model
R2077	T10272	T10273	det	the,cell
R2078	T10273	T10271	pobj	cell,throughout
R2079	T10274	T10259	prep	in,distributed
R2080	T10275	T10276	det	the,state
R2081	T10182	T10184	nsubj	model,affords
R2082	T10276	T10274	pobj	state,in
R2083	T10277	T10276	amod	basal,state
R2084	T10278	T10279	punct	(,row
R2085	T10183	T10182	compound	mouse,model
R2086	T10185	T10182	prep	of,model
R2087	T10279	T10259	parataxis	row,distributed
R2088	T10186	T10185	pobj	NDI,of
R2089	T10280	T10279	dep	Figure,row
R2090	T10281	T10280	nummod	4A,Figure
R2091	T10187	T10188	det	the,opportunity
R2092	T10282	T10279	punct	", ",row
R2093	T10283	T10279	amod	third,row
R2094	T10284	T10279	punct	),row
R2095	T10188	T10184	dobj	opportunity,affords
R2096	T10285	T10259	punct	", ",distributed
R2097	T10286	T10287	mark	while,localized
R2098	T10189	T10188	amod	first,opportunity
R2099	T10287	T10259	advcl	localized,distributed
R2100	T10288	T10287	nsubj	AQP3,localized
R2101	T10289	T10290	punct	(,green
R2102	T10290	T10288	parataxis	green,AQP3
R2103	T10291	T10290	punct	),green
R2104	T10190	T10191	aux	to,test
R2105	T10292	T10287	advmod	appropriately,localized
R2106	T10293	T10287	prep	to,localized
R2107	T10294	T10295	det	the,surface
R2108	T10191	T10188	acl	test,opportunity
R2109	T10295	T10293	pobj	surface,to
R2110	T10296	T10295	amod	basolateral,surface
R2111	T10297	T10298	punct	[,23
R2112	T10192	T10193	det	this,hypothesis
R2113	T10298	T10287	parataxis	23,localized
R2114	T10299	T10298	punct	],23
R2115	T10300	T10259	punct	.,distributed
R2116	T10193	T10191	dobj	hypothesis,test
R2117	T10302	T10303	advmod	Furthermore,failed
R2118	T10194	T10191	prep	in,test
R2119	T10304	T10303	punct	", ",failed
R2120	T10305	T10303	prep	upon,failed
R2121	T10306	T10307	compound	dDAVP,stimulation
R2122	T10195	T10196	det	a,animal
R2123	T10307	T10305	pobj	stimulation,upon
R2124	T10308	T10303	punct	", ",failed
R2125	T10309	T10310	compound	AQP2,F204V
R2126	T10196	T10194	pobj	animal,in
R2127	T10310	T10303	nsubj	F204V,failed
R2128	T10311	T10310	punct	-,F204V
R2129	T10312	T10313	aux	to,translocate
R2130	T10197	T10196	amod	mature,animal
R2131	T10313	T10303	xcomp	translocate,failed
R2132	T10314	T10313	prep	to,translocate
R2133	T10198	T10184	punct	.,affords
R2134	T10315	T10316	det	the,surface
R2135	T10316	T10314	pobj	surface,to
R2136	T10317	T10316	compound	cell,surface
R2137	T10200	T10201	mark	As,shown
R2138	T10318	T10319	punct	(,row
R2139	T10319	T10303	parataxis	row,failed
R2140	T10320	T10319	dep	Figure,row
R2141	T10321	T10320	nummod	4A,Figure
R2142	T10322	T10319	punct	", ",row
R2143	T10201	T10202	advcl	shown,localized
R2144	T10323	T10319	amod	bottom,row
R2145	T10324	T10319	punct	),row
R2146	T10203	T10201	prep	in,shown
R2147	T10325	T10303	punct	.,failed
R2148	T10327	T10328	aux	To,confirm
R2149	T10328	T10329	advcl	confirm,repeated
R2150	T10204	T10203	pobj	Figure,in
R2151	T10330	T10331	det	these,findings
R2152	T10331	T10328	dobj	findings,confirm
R2153	T10332	T10329	punct	", ",repeated
R2154	T10333	T10334	det	the,staining
R2155	T10334	T10329	nsubjpass	staining,repeated
R2156	T10205	T10204	nummod	4A,Figure
R2157	T10335	T10329	auxpass	was,repeated
R2158	T10336	T10329	prep	in,repeated
R2159	T10337	T10336	pobj	kidneys,in
R2160	T10206	T10207	punct	(,row
R2161	T10338	T10337	acl	taken,kidneys
R2162	T10339	T10338	prep	from,taken
R2163	T10340	T10341	nummod	two,mice
R2164	T10341	T10339	pobj	mice,from
R2165	T10207	T10204	parataxis	row,Figure
R2166	T10342	T10341	amod	further,mice
R2167	T10343	T10338	prep	for,taken
R2168	T10208	T10207	amod	top,row
R2169	T10344	T10345	det	each,class
R2170	T10345	T10343	pobj	class,for
R2171	T10209	T10207	prep	of,row
R2172	T10346	T10345	punct	", ",class
R2173	T10347	T10348	amod	wild,type
R2174	T10348	T10345	appos	type,class
R2175	T10210	T10209	pobj	photomicrographs,of
R2176	T10349	T10348	punct	-,type
R2177	T10350	T10348	cc	or,type
R2178	T10351	T10348	conj	mutant,type
R2179	T10211	T10207	punct	),row
R2180	T10352	T10329	punct	", ",repeated
R2181	T10353	T10329	prep	with,repeated
R2182	T10354	T10353	cc	or,with
R2183	T10212	T10202	punct	", ",localized
R2184	T10355	T10353	conj	without,with
R2185	T10213	T10202	nsubj	AQP2,localized
R2186	T10214	T10213	punct	(,AQP2
R2187	T10215	T10213	acl	stained,AQP2
R2188	T10356	T10357	compound	dDAVP,treatment
R2189	T10357	T10355	pobj	treatment,without
R2190	T10216	T10215	oprd	red,stained
R2191	T10358	T10329	punct	", ",repeated
R2192	T10359	T10329	prep	with,repeated
R2193	T10217	T10213	punct	),AQP2
R2194	T10360	T10361	amod	identical,results
R2195	T10361	T10359	pobj	results,with
R2196	T10362	T10329	punct	.,repeated
R2197	T10218	T10202	advmod	normally,localized
R2198	T10364	T10365	aux	To,investigate
R2199	T10365	T10366	advcl	investigate,turned
R2200	T10219	T10202	prep	to,localized
R2201	T10367	T10368	det	the,mechanism
R2202	T10220	T10221	det	the,region
R2203	T10368	T10365	dobj	mechanism,investigate
R2204	T10221	T10219	pobj	region,to
R2205	T10369	T10368	prep	of,mechanism
R2206	T10370	T10371	amod	defective,translocation
R2207	T10222	T10221	amod	subapical,region
R2208	T10371	T10369	pobj	translocation,of
R2209	T10372	T10371	prep	of,translocation
R2210	T10223	T10221	prep	of,region
R2211	T10373	T10374	compound	AQP2,F204V
R2212	T10374	T10372	pobj	F204V,of
R2213	T10375	T10374	punct	-,F204V
R2214	T10224	T10225	amod	collecting,duct
R2215	T10376	T10366	punct	", ",turned
R2216	T10377	T10366	nsubj	we,turned
R2217	T10225	T10226	compound	duct,cells
R2218	T10378	T10366	prep	to,turned
R2219	T10379	T10378	pobj	transfection,to
R2220	T10226	T10223	pobj	cells,of
R2221	T10380	T10379	prep	of,transfection
R2222	T10381	T10382	compound	MDCK,cells
R2223	T10382	T10380	pobj	cells,of
R2224	T10227	T10221	prep	in,region
R2225	T10383	T10366	punct	.,turned
R2226	T10228	T10227	pobj	kidneys,in
R2227	T10385	T10386	amod	Stable,lines
R2228	T10386	T10388	nsubjpass	lines,established
R2229	T10387	T10386	compound	cell,lines
R2230	T10229	T10228	prep	of,kidneys
R2231	T10389	T10386	acl	expressing,lines
R2232	T10390	T10391	nmod	mouse,AQP2
R2233	T10230	T10231	amod	wild,type
R2234	T10391	T10389	dobj	AQP2,expressing
R2235	T10392	T10393	amod	wild,type
R2236	T10393	T10391	compound	type,AQP2
R2237	T10231	T10233	compound	type,mice
R2238	T10394	T10393	punct	-,type
R2239	T10395	T10391	cc	and,AQP2
R2240	T10232	T10231	punct	-,type
R2241	T10396	T10397	compound	AQP2,F204V
R2242	T10397	T10391	conj	F204V,AQP2
R2243	T10398	T10397	punct	-,F204V
R2244	T10233	T10229	pobj	mice,of
R2245	T10399	T10388	auxpass	were,established
R2246	T10400	T10388	punct	.,established
R2247	T10234	T10202	punct	.,localized
R2248	T10402	T10403	nsubj	Immunoblots,showed
R2249	T10236	T10237	prep	Upon,translocated
R2250	T10404	T10402	prep	of,Immunoblots
R2251	T10405	T10406	compound	protein,extracts
R2252	T10406	T10404	pobj	extracts,of
R2253	T10407	T10406	prep	from,extracts
R2254	T10408	T10409	amod	stable,lines
R2255	T10409	T10407	pobj	lines,from
R2256	T10410	T10409	compound	cell,lines
R2257	T10238	T10236	pobj	stimulation,Upon
R2258	T10411	T10412	mark	that,recapitulate
R2259	T10412	T10403	ccomp	recapitulate,showed
R2260	T10413	T10414	compound	MDCK,cells
R2261	T10414	T10412	nsubj	cells,recapitulate
R2262	T10415	T10416	det	the,defect
R2263	T10239	T10238	prep	with,stimulation
R2264	T10416	T10412	dobj	defect,recapitulate
R2265	T10417	T10416	compound	glycosylation,defect
R2266	T10240	T10239	pobj	dDAVP,with
R2267	T10418	T10416	acl	seen,defect
R2268	T10419	T10418	prep	in,seen
R2269	T10420	T10421	compound	mutant,mice
R2270	T10241	T10237	punct	", ",translocated
R2271	T10421	T10419	pobj	mice,in
R2272	T10422	T10423	punct	(,data
R2273	T10423	T10403	meta	data,showed
R2274	T10424	T10423	amod	unpublished,data
R2275	T10242	T10237	nsubj	AQP2,translocated
R2276	T10425	T10423	punct	),data
R2277	T10426	T10403	punct	.,showed
R2278	T10243	T10237	prep	to,translocated
R2279	T10428	T10429	det	The,protein
R2280	T10429	T10433	nsubj	protein,was
R2281	T10244	T10243	cc	or,to
R2282	T10430	T10431	amod	wild,type
R2283	T10431	T10429	compound	type,protein
R2284	T10432	T10431	punct	-,type
R2285	T10245	T10243	conj	near,to
R2286	T10434	T10433	advmod	again,was
R2287	T10246	T10247	det	the,surface
R2288	T10435	T10433	acomp	present,was
R2289	T10436	T10433	prep	in,was
R2290	T10437	T10438	nummod	three,forms
R2291	T10247	T10245	pobj	surface,near
R2292	T10438	T10436	pobj	forms,in
R2293	T10439	T10438	amod	different,forms
R2294	T10248	T10247	compound	cell,surface
R2295	T10440	T10433	punct	.,was
R2296	T10442	T10443	nsubj	Cells,lacked
R2297	T10249	T10250	punct	(,row
R2298	T10444	T10442	acl	expressing,Cells
R2299	T10445	T10446	compound	AQP2,F204V
R2300	T10462	T10461	punct	-,glycosylated
R2301	T10446	T10444	dobj	F204V,expressing
R2302	T10447	T10446	punct	-,F204V
R2303	T10448	T10449	det	the,form
R2304	T10449	T10443	dobj	form,lacked
R2305	T10463	T10464	nummod	31,kDa
R2306	T10450	T10451	quantmod	35,45
R2307	T10451	T10453	nummod	45,kDa
R2308	T10452	T10451	punct	–,45
R2309	T10453	T10449	compound	kDa,form
R2310	T10454	T10443	cc	and,lacked
R2311	T10455	T10456	auxpass	were,enriched
R2312	T10464	T10459	compound	kDa,form
R2313	T10456	T10443	conj	enriched,lacked
R2314	T10457	T10456	prep	in,enriched
R2315	T10465	T10443	punct	.,lacked
R2316	T10458	T10459	det	the,form
R2317	T10467	T10468	prep	In,appeared
R2318	T10459	T10457	pobj	form,in
R2319	T10460	T10461	npadvmod	core,glycosylated
R2320	T10461	T10459	amod	glycosylated,form
R2321	T10469	T10470	amod	transfected,cells
R2322	T10470	T10467	pobj	cells,In
R2323	T10471	T10470	punct	", ",cells
R2324	T10567	T10568	prep	Along,seen
R2325	T10472	T10470	amod	unstimulated,cells
R2326	T10569	T10570	det	the,axis
R2327	T10570	T10567	pobj	axis,Along
R2328	T10473	T10470	compound	MDCK,cells
R2329	T10571	T10570	compound	z,axis
R2330	T10572	T10570	punct	-,axis
R2331	T10573	T10568	punct	", ",seen
R2332	T10474	T10468	punct	", ",appeared
R2333	T10574	T10575	det	the,distribution
R2334	T10475	T10476	amod	wild,type
R2335	T10476	T10478	compound	type,AQP2
R2336	T10575	T10568	nsubjpass	distribution,seen
R2337	T10576	T10575	amod	perinuclear,distribution
R2338	T10477	T10476	punct	-,type
R2339	T10577	T10575	prep	of,distribution
R2340	T10578	T10579	compound	AQP2,F204V
R2341	T10478	T10468	nsubj	AQP2,appeared
R2342	T10579	T10577	pobj	F204V,of
R2343	T10580	T10579	punct	-,F204V
R2344	T10581	T10568	auxpass	was,seen
R2345	T10582	T10568	advmod	clearly,seen
R2346	T10479	T10478	punct	(,AQP2
R2347	T10583	T10568	punct	", ",seen
R2348	T10584	T10568	cc	and,seen
R2349	T10585	T10586	det	this,distribution
R2350	T10480	T10478	acl	stained,AQP2
R2351	T10586	T10587	nsubjpass	distribution,altered
R2352	T10587	T10568	conj	altered,seen
R2353	T10588	T10587	auxpass	is,altered
R2354	T10481	T10480	oprd	green,stained
R2355	T10589	T10587	neg	not,altered
R2356	T10590	T10587	agent	by,altered
R2357	T10482	T10478	punct	),AQP2
R2358	T10591	T10590	pobj	forskolin,by
R2359	T10592	T10593	punct	(,columns
R2360	T10593	T10587	parataxis	columns,altered
R2361	T10594	T10593	dep	Figure,columns
R2362	T10595	T10594	nummod	4B,Figure
R2363	T10596	T10593	punct	", ",columns
R2364	T10483	T10468	prep	in,appeared
R2365	T10597	T10598	amod	bottom,row
R2366	T10598	T10593	dep	row,columns
R2367	T10599	T10593	punct	", ",columns
R2368	T10484	T10485	det	a,pattern
R2369	T10600	T10593	nummod	two,columns
R2370	T10601	T10593	amod	right,columns
R2371	T10602	T10593	punct	),columns
R2372	T10485	T10483	pobj	pattern,in
R2373	T10603	T10587	punct	.,altered
R2374	T10486	T10485	compound	punctate,pattern
R2375	T10605	T10606	det	The,distribution
R2376	T10606	T10608	nsubj	distribution,is
R2377	T10607	T10606	amod	perinuclear,distribution
R2378	T10487	T10485	acl	distributed,pattern
R2379	T10609	T10606	prep	of,distribution
R2380	T10610	T10611	compound	AQP2,F204V
R2381	T10488	T10487	prep	throughout,distributed
R2382	T10611	T10609	pobj	F204V,of
R2383	T10612	T10611	punct	-,F204V
R2384	T10489	T10490	det	the,region
R2385	T10613	T10608	acomp	consistent,is
R2386	T10614	T10613	prep	with,consistent
R2387	T10615	T10616	det	an,compartmentalization
R2388	T10616	T10614	pobj	compartmentalization,with
R2389	T10490	T10488	pobj	region,throughout
R2390	T10617	T10616	compound	ER,compartmentalization
R2391	T10618	T10608	punct	.,is
R2392	T10491	T10490	amod	subapical,region
R2393	T10620	T10621	aux	To,test
R2394	T10621	T10622	advcl	test,co-stained
R2395	T10492	T10493	punct	(,photomicrographs
R2396	T10623	T10624	det	the,idea
R2397	T10493	T10468	parataxis	photomicrographs,appeared
R2398	T10624	T10621	dobj	idea,test
R2399	T10625	T10626	mark	that,localizes
R2400	T10626	T10624	acl	localizes,idea
R2401	T10494	T10493	dep	Figure,photomicrographs
R2402	T10627	T10628	compound	AQP2,F204V
R2403	T10628	T10626	nsubj	F204V,localizes
R2404	T10629	T10628	punct	-,F204V
R2405	T10495	T10494	nummod	4B,Figure
R2406	T10630	T10626	prep	to,localizes
R2407	T10631	T10632	det	the,ER
R2408	T10496	T10493	punct	", ",photomicrographs
R2409	T10497	T10498	amod	left,column
R2410	T10632	T10630	pobj	ER,to
R2411	T10633	T10622	punct	", ",co-stained
R2412	T10634	T10622	nsubj	we,co-stained
R2413	T10635	T10622	dobj	cells,co-stained
R2414	T10636	T10635	acl	transfected,cells
R2415	T10498	T10493	compound	column,photomicrographs
R2416	T10637	T10636	prep	with,transfected
R2417	T10638	T10637	pobj	Aqp2F204V,with
R2418	T10499	T10493	punct	),photomicrographs
R2419	T10639	T10640	punct	(,cDNA
R2420	T10640	T10638	parataxis	cDNA,Aqp2F204V
R2421	T10500	T10468	punct	", ",appeared
R2422	T10641	T10640	punct	),cDNA
R2423	T10642	T10622	prep	for,co-stained
R2424	T10643	T10642	pobj	AQP2,for
R2425	T10501	T10468	advcl	consistent,appeared
R2426	T10644	T10643	cc	and,AQP2
R2427	T10645	T10646	det	an,marker
R2428	T10646	T10643	conj	marker,AQP2
R2429	T10502	T10501	prep	with,consistent
R2430	T10647	T10646	compound	ER,marker
R2431	T10648	T10646	punct	", ",marker
R2432	T10649	T10646	appos	calnexin,marker
R2433	T10503	T10504	amod	vesicular,compartmentalization
R2434	T10650	T10651	punct	(,Figure
R2435	T10651	T10622	parataxis	Figure,co-stained
R2436	T10652	T10651	nummod	4C,Figure
R2437	T10504	T10502	pobj	compartmentalization,with
R2438	T10653	T10651	punct	),Figure
R2439	T10654	T10622	punct	.,co-stained
R2440	T10505	T10468	punct	.,appeared
R2441	T10656	T10657	nsubjpass	Colocalization,investigated
R2442	T10658	T10656	prep	of,Colocalization
R2443	T10507	T10508	compound	AQP2,F204V
R2444	T10659	T10658	pobj	calnexin,of
R2445	T10660	T10656	prep	with,Colocalization
R2446	T10661	T10660	pobj	AQP2,with
R2447	T10662	T10657	auxpass	was,investigated
R2448	T10508	T10510	nsubj	F204V,appeared
R2449	T10663	T10657	advmod	directly,investigated
R2450	T10664	T10657	punct	", ",investigated
R2451	T10665	T10657	cc	and,investigated
R2452	T10509	T10508	punct	-,F204V
R2453	T10666	T10667	nsubjpass	it,found
R2454	T10667	T10657	conj	found,investigated
R2455	T10668	T10667	auxpass	was,found
R2456	T10511	T10510	punct	", ",appeared
R2457	T10669	T10670	mark	that,colocalized
R2458	T10670	T10667	ccomp	colocalized,found
R2459	T10671	T10672	nummod	80,%
R2460	T10672	T10670	nsubj	%,colocalized
R2461	T10512	T10510	prep	on,appeared
R2462	T10513	T10514	det	the,hand
R2463	T10673	T10672	prep	of,%
R2464	T10514	T10512	pobj	hand,on
R2465	T10674	T10675	det	all,protein
R2466	T10675	T10673	pobj	protein,of
R2467	T10676	T10677	compound	AQP2,F204V
R2468	T10515	T10514	amod	other,hand
R2469	T10677	T10675	compound	F204V,protein
R2470	T10678	T10677	punct	-,F204V
R2471	T10679	T10670	prep	with,colocalized
R2472	T10516	T10510	punct	", ",appeared
R2473	T10680	T10679	pobj	calnexin,with
R2474	T10681	T10667	punct	.,found
R2475	T10517	T10510	prep	in,appeared
R2476	T10683	T10684	det	The,%
R2477	T10684	T10687	nsubj	%,appeared
R2478	T10518	T10519	det	a,pattern
R2479	T10685	T10684	amod	remaining,%
R2480	T10686	T10684	nummod	20,%
R2481	T10519	T10517	pobj	pattern,in
R2482	T10688	T10687	prep	at,appeared
R2483	T10689	T10690	det	the,periphery
R2484	T10520	T10521	npadvmod	punctate,perinuclear
R2485	T10690	T10688	pobj	periphery,at
R2486	T10691	T10690	prep	of,periphery
R2487	T10692	T10693	det	the,ER
R2488	T10521	T10519	amod	perinuclear,pattern
R2489	T10693	T10691	pobj	ER,of
R2490	T10694	T10687	punct	", ",appeared
R2491	T10695	T10687	advcl	representing,appeared
R2492	T10522	T10521	cc	but,perinuclear
R2493	T10696	T10697	compound	AQP2,F204V
R2494	T10697	T10695	dobj	F204V,representing
R2495	T10698	T10697	punct	-,F204V
R2496	T10523	T10524	punct	(,column
R2497	T10699	T10700	dep	that,progressed
R2498	T10700	T10697	relcl	progressed,F204V
R2499	T10524	T10510	parataxis	column,appeared
R2500	T10701	T10700	aux	had,progressed
R2501	T10702	T10700	advmod	potentially,progressed
R2502	T10703	T10700	prep	beyond,progressed
R2503	T10525	T10524	dep	Figure,column
R2504	T10704	T10705	det	the,ER
R2505	T10705	T10703	pobj	ER,beyond
R2506	T10706	T10687	punct	.,appeared
R2507	T10526	T10525	nummod	4B,Figure
R2508	T10708	T10709	det	This,escape
R2509	T10709	T10712	nsubj	escape,was
R2510	T10527	T10524	punct	", ",column
R2511	T10710	T10709	punct	“,escape
R2512	T10711	T10709	compound	ER,escape
R2513	T10528	T10524	amod	third,column
R2514	T10713	T10709	punct	”,escape
R2515	T10714	T10712	acomp	consistent,was
R2516	T10715	T10714	prep	with,consistent
R2517	T10716	T10717	det	the,proportion
R2518	T10717	T10715	pobj	proportion,with
R2519	T10529	T10524	punct	),column
R2520	T10718	T10717	amod	small,proportion
R2521	T10530	T10510	punct	.,appeared
R2522	T10719	T10717	prep	of,proportion
R2523	T10720	T10721	amod	mature,F204V
R2524	T10721	T10719	pobj	F204V,of
R2525	T10532	T10533	prep	Upon,translocated
R2526	T10722	T10721	punct	", ",F204V
R2527	T10723	T10724	amod	complex,glycosylated
R2528	T10724	T10721	amod	glycosylated,F204V
R2529	T10725	T10721	punct	", ",F204V
R2530	T10534	T10532	pobj	stimulation,Upon
R2531	T10726	T10721	compound	AQP2,F204V
R2532	T10727	T10721	punct	-,F204V
R2533	T10728	T10717	prep	in,proportion
R2534	T10535	T10534	prep	with,stimulation
R2535	T10729	T10730	compound	mutant,kidneys
R2536	T10730	T10728	pobj	kidneys,in
R2537	T10731	T10732	punct	(,see
R2538	T10536	T10535	pobj	forskolin,with
R2539	T10732	T10712	parataxis	see,was
R2540	T10733	T10732	dobj	Figure,see
R2541	T10537	T10533	punct	", ",translocated
R2542	T10734	T10733	nummod	3A,Figure
R2543	T10735	T10732	punct	),see
R2544	T10736	T10712	punct	.,was
R2545	T10538	T10539	det	a,activator
R2546	T10738	T10739	nsubjpass	Animals,affected
R2547	T10539	T10533	nsubj	activator,translocated
R2548	T10740	T10738	amod	heterozygous,Animals
R2549	T10741	T10740	prep	for,heterozygous
R2550	T10742	T10743	det	the,mutation
R2551	T10540	T10541	npadvmod	cAMP,dependent
R2552	T10743	T10741	pobj	mutation,for
R2553	T10744	T10743	compound	Aqp2F204V,mutation
R2554	T10745	T10739	auxpass	were,affected
R2555	T10541	T10539	amod	dependent,activator
R2556	T10746	T10739	neg	not,affected
R2557	T10747	T10739	prep	in,affected
R2558	T10748	T10749	poss	their,production
R2559	T10542	T10541	punct	-,dependent
R2560	T10749	T10747	pobj	production,in
R2561	T10750	T10749	compound	urine,production
R2562	T10751	T10749	cc	or,production
R2563	T10543	T10544	compound	protein,kinase
R2564	T10752	T10753	compound	urine,osmolality
R2565	T10753	T10749	conj	osmolality,production
R2566	T10754	T10755	punct	(,see
R2567	T10755	T10739	parataxis	see,affected
R2568	T10756	T10757	nmod	Figure,1C
R2569	T10757	T10755	dobj	1C,see
R2570	T10544	T10539	compound	kinase,activator
R2571	T10758	T10757	cc	and,1C
R2572	T10759	T10757	conj	1D,1C
R2573	T10760	T10755	punct	),see
R2574	T10545	T10539	punct	", ",activator
R2575	T10761	T10739	punct	.,affected
R2576	T10763	T10764	nsubjpass	It,shown
R2577	T10546	T10547	amod	wild,type
R2578	T10765	T10764	aux	has,shown
R2579	T10766	T10764	advmod	also,shown
R2580	T10547	T10549	compound	type,AQP2
R2581	T10767	T10764	auxpass	been,shown
R2582	T10768	T10769	mark	that,interact
R2583	T10769	T10764	ccomp	interact,shown
R2584	T10548	T10547	punct	-,type
R2585	T10770	T10771	det	a,allele
R2586	T10771	T10769	nsubj	allele,interact
R2587	T10772	T10771	amod	recessive,allele
R2588	T10549	T10539	appos	AQP2,activator
R2589	T10773	T10771	compound	NDI,allele
R2590	T10774	T10771	punct	", ",allele
R2591	T10775	T10776	compound	AQP2,R187C
R2592	T10550	T10533	prep	to,translocated
R2593	T10776	T10771	appos	R187C,allele
R2594	T10777	T10776	punct	-,R187C
R2595	T10778	T10769	punct	", ",interact
R2596	T10551	T10552	det	the,surface
R2597	T10552	T10550	pobj	surface,to
R2598	T10553	T10552	amod	apical,surface
R2599	T10779	T10769	aux	does,interact
R2600	T10554	T10552	prep	of,surface
R2601	T10780	T10769	neg	not,interact
R2602	T10781	T10769	prep	with,interact
R2603	T10782	T10783	amod	wild,type
R2604	T10555	T10556	amod	polarized,cells
R2605	T10783	T10785	compound	type,protein
R2606	T10784	T10783	punct	-,type
R2607	T10556	T10554	pobj	cells,of
R2608	T10785	T10781	pobj	protein,with
R2609	T10786	T10769	prep	in,interact
R2610	T10787	T10786	pobj	oocytes,in
R2611	T10557	T10556	compound	MDCK,cells
R2612	T10788	T10789	punct	[,24
R2613	T10789	T10769	parataxis	24,interact
R2614	T10790	T10789	punct	],24
R2615	T10558	T10559	punct	(,column
R2616	T10791	T10769	punct	", ",interact
R2617	T10792	T10769	cc	nor,interact
R2618	T10793	T10794	aux	does,homo-oligomerize
R2619	T10794	T10769	conj	homo-oligomerize,interact
R2620	T10795	T10794	nsubj	it,homo-oligomerize
R2621	T10796	T10794	prep	in,homo-oligomerize
R2622	T10559	T10533	parataxis	column,translocated
R2623	T10797	T10798	compound	MDCK,cells
R2624	T10798	T10796	pobj	cells,in
R2625	T10799	T10800	punct	[,22
R2626	T10560	T10559	dep	Figure,column
R2627	T10800	T10764	parataxis	22,shown
R2628	T10801	T10800	punct	],22
R2629	T10802	T10764	punct	.,shown
R2630	T10561	T10560	nummod	4B,Figure
R2631	T10804	T10805	advmod	Therefore,examined
R2632	T10562	T10559	punct	", ",column
R2633	T10563	T10559	amod	second,column
R2634	T10806	T10805	punct	", ",examined
R2635	T10564	T10559	punct	),column
R2636	T10807	T10805	nsubjpass	kidneys,examined
R2637	T10808	T10807	prep	from,kidneys
R2638	T10565	T10533	punct	.,translocated
R2639	T10809	T10810	amod	heterozygous,animals
R2640	T10810	T10808	pobj	animals,from
R2641	T10811	T10805	auxpass	were,examined
R2642	T10885	T10884	prep	by,undetectable
R2643	T10812	T10805	prep	for,examined
R2644	T10813	T10812	pobj	evidence,for
R2645	T10814	T10813	prep	of,evidence
R2646	T10815	T10816	nummod	two,populations
R2647	T10886	T10885	pobj	immunocytochemistry,by
R2648	T10816	T10814	pobj	populations,of
R2649	T10817	T10816	prep	of,populations
R2650	T10818	T10819	compound	AQP2,protein
R2651	T10887	T10868	punct	.,reflect
R2652	T10819	T10817	pobj	protein,of
R2653	T10820	T10805	punct	.,examined
R2654	T10889	T10890	advmod	Alternatively,alter
R2655	T10822	T10823	advmod	Surprisingly,revealed
R2656	T10824	T10823	punct	", ",revealed
R2657	T10891	T10890	punct	", ",alter
R2658	T10825	T10826	amod	immunohistochemical,staining
R2659	T10826	T10823	nsubj	staining,revealed
R2660	T10827	T10826	prep	of,staining
R2661	T10828	T10829	nmod	kidney,ducts
R2662	T10892	T10893	det	the,presence
R2663	T10829	T10827	pobj	ducts,of
R2664	T10830	T10829	amod	collecting,ducts
R2665	T10831	T10829	prep	from,ducts
R2666	T10893	T10890	nsubj	presence,alter
R2667	T10832	T10833	nmod	Aqp2F204V,mice
R2668	T10833	T10831	pobj	mice,from
R2669	T10834	T10832	punct	/,Aqp2F204V
R2670	T10835	T10832	punct	+,Aqp2F204V
R2671	T10836	T10837	det	a,pattern
R2672	T10837	T10823	dobj	pattern,revealed
R2673	T10894	T10893	prep	of,presence
R2674	T10838	T10839	advmod	remarkably,similar
R2675	T10839	T10837	amod	similar,pattern
R2676	T10840	T10839	prep	to,similar
R2677	T10895	T10896	amod	wild,type
R2678	T10841	T10842	amod	wild,type
R2679	T10842	T10840	pobj	type,to
R2680	T10843	T10844	punct	(,Figure
R2681	T10844	T10823	parataxis	Figure,revealed
R2682	T10845	T10844	nummod	5A,Figure
R2683	T10846	T10844	punct	),Figure
R2684	T10847	T10823	punct	.,revealed
R2685	T10896	T10898	compound	type,protein
R2686	T10849	T10850	nsubj	AQP2,translocated
R2687	T10897	T10896	punct	-,type
R2688	T10851	T10850	advmod	completely,translocated
R2689	T10852	T10850	prep	to,translocated
R2690	T10898	T10894	pobj	protein,of
R2691	T10853	T10854	det	the,surface
R2692	T10854	T10852	pobj	surface,to
R2693	T10855	T10854	amod	apical,surface
R2694	T10856	T10854	compound	cell,surface
R2695	T10899	T10890	aux	may,alter
R2696	T10857	T10850	prep	upon,translocated
R2697	T10858	T10859	compound	dDAVP,stimulation
R2698	T10859	T10857	pobj	stimulation,upon
R2699	T10900	T10901	det	the,localization
R2700	T10860	T10850	punct	.,translocated
R2701	T10901	T10890	dobj	localization,alter
R2702	T10862	T10863	det	This,pattern
R2703	T10863	T10868	nsubj	pattern,reflect
R2704	T10864	T10865	amod	wild,type
R2705	T10902	T10901	prep	of,localization
R2706	T10865	T10863	compound	type,pattern
R2707	T10866	T10865	punct	-,type
R2708	T10867	T10863	compound	staining,pattern
R2709	T10903	T10904	det	the,protein
R2710	T10869	T10868	aux	may,reflect
R2711	T10904	T10902	pobj	protein,of
R2712	T10870	T10868	advmod	simply,reflect
R2713	T10871	T10872	det	the,fact
R2714	T10872	T10868	dobj	fact,reflect
R2715	T10873	T10874	mark	that,makes
R2716	T10905	T10904	compound	mutant,protein
R2717	T10874	T10872	acl	makes,fact
R2718	T10875	T10874	csubj	decreasing,makes
R2719	T10876	T10877	det	the,amount
R2720	T10906	T10890	punct	.,alter
R2721	T10877	T10875	dobj	amount,decreasing
R2722	T10878	T10877	prep	of,amount
R2723	T10879	T10880	compound	mutant,protein
R2724	T10880	T10878	pobj	protein,of
R2725	T10881	T10875	prep	by,decreasing
R2726	T10882	T10881	pobj	half,by
R2727	T10908	T10909	advmod	Indeed,proposed
R2728	T10883	T10884	nsubj	it,undetectable
R2729	T10884	T10874	ccomp	undetectable,makes
R2730	T10910	T10909	punct	", ",proposed
R2731	T10911	T10909	nsubj	Hendriks,proposed
R2732	T10912	T10913	advmod	et,al.
R2733	T10913	T10911	advmod	al.,Hendriks
R2734	T10991	T10980	nsubj	type,rescue
R2735	T10914	T10915	det	a,mechanism
R2736	T10992	T10991	amod	wild,type
R2737	T10915	T10909	dobj	mechanism,proposed
R2738	T10993	T10980	aux	may,rescue
R2739	T10994	T10995	det	the,protein
R2740	T10916	T10915	punct	“,mechanism
R2741	T10995	T10980	dobj	protein,rescue
R2742	T10996	T10995	compound	mutant,protein
R2743	T10997	T10998	mark	as,suggested
R2744	T10917	T10918	nmod	piggy,back
R2745	T10998	T10980	advcl	suggested,rescue
R2746	T10999	T10998	agent	by,suggested
R2747	T10918	T10915	nmod	back,mechanism
R2748	T11000	T11001	det	the,distribution
R2749	T11001	T10999	pobj	distribution,by
R2750	T11002	T11001	amod	subcellular,distribution
R2751	T11003	T11001	prep	of,distribution
R2752	T10919	T10918	punct	-,back
R2753	T11004	T11005	compound	AQP2,protein
R2754	T11005	T11003	pobj	protein,of
R2755	T10920	T10915	punct	”,mechanism
R2756	T11006	T10980	punct	.,rescue
R2757	T11008	T11009	aux	To,test
R2758	T10921	T10922	aux	to,explain
R2759	T11009	T11010	advcl	test,looked
R2760	T10922	T10909	advcl	explain,proposed
R2761	T11011	T11012	det	this,idea
R2762	T11012	T11009	dobj	idea,test
R2763	T11013	T11010	punct	", ",looked
R2764	T11014	T11010	nsubj	we,looked
R2765	T10923	T10924	det	the,transport
R2766	T11015	T11010	advmod	first,looked
R2767	T11016	T11010	prep	for,looked
R2768	T10924	T10922	dobj	transport,explain
R2769	T11017	T11018	det	an,interaction
R2770	T11018	T11016	pobj	interaction,for
R2771	T11019	T11018	prep	between,interaction
R2772	T10925	T10924	prep	of,transport
R2773	T11020	T11021	nmod	mutant,proteins
R2774	T11021	T11019	pobj	proteins,between
R2775	T11022	T11020	cc	and,mutant
R2776	T11023	T11024	amod	wild,type
R2777	T11024	T11020	conj	type,mutant
R2778	T10926	T10927	amod	nonglycosylated,subunits
R2779	T11025	T11024	punct	-,type
R2780	T11026	T11010	prep	in,looked
R2781	T11027	T11028	amod	transfected,cells
R2782	T10927	T10925	pobj	subunits,of
R2783	T11028	T11026	pobj	cells,in
R2784	T11029	T11028	compound	MDCK,cells
R2785	T11030	T11031	punct	(,Figure
R2786	T10928	T10927	prep	of,subunits
R2787	T11031	T11010	parataxis	Figure,looked
R2788	T11032	T11031	nummod	5B,Figure
R2789	T11033	T11031	punct	),Figure
R2790	T11034	T11010	punct	.,looked
R2791	T10929	T10928	pobj	AQP2,of
R2792	T11036	T11037	compound	MDCK,cells
R2793	T10930	T10924	prep	to,transport
R2794	T11037	T11038	nsubjpass	cells,transfected
R2795	T11039	T11040	advmod	stably,expressing
R2796	T10931	T10932	det	the,surface
R2797	T11040	T11037	acl	expressing,cells
R2798	T11041	T11042	amod	wild,type
R2799	T10932	T10930	pobj	surface,to
R2800	T11042	T11044	compound	type,AQP2
R2801	T11043	T11042	punct	-,type
R2802	T11044	T11040	dobj	AQP2,expressing
R2803	T10933	T10932	compound	cell,surface
R2804	T11045	T11038	auxpass	were,transfected
R2805	T11046	T11038	advmod	transiently,transfected
R2806	T11047	T11038	prep	with,transfected
R2807	T10934	T10924	prep	by,transport
R2808	T11048	T11049	compound	GFP,constructs
R2809	T11049	T11047	pobj	constructs,with
R2810	T11050	T11049	compound	expression,constructs
R2811	T10935	T10936	amod	glycosylated,subunits
R2812	T11051	T11049	acl	encoding,constructs
R2813	T11052	T11053	npadvmod	GFP,tagged
R2814	T10936	T10934	pobj	subunits,by
R2815	T11053	T11055	amod	tagged,AQP2
R2816	T11054	T11053	punct	-,tagged
R2817	T11055	T11051	dobj	AQP2,encoding
R2818	T10937	T10938	punct	[,22
R2819	T11056	T11057	amod	wild,type
R2820	T11057	T11055	compound	type,AQP2
R2821	T11058	T11057	punct	-,type
R2822	T10938	T10922	parataxis	22,explain
R2823	T11059	T11055	punct	", ",AQP2
R2824	T11060	T11061	compound	AQP2,F204V
R2825	T11061	T11055	conj	F204V,AQP2
R2826	T11062	T11061	punct	-,F204V
R2827	T11063	T11061	punct	", ",F204V
R2828	T11064	T11061	cc	or,F204V
R2829	T10939	T10938	punct	],22
R2830	T11065	T11061	conj	GFP,F204V
R2831	T11066	T11065	advmod	alone,GFP
R2832	T10940	T10909	punct	.,proposed
R2833	T10942	T10943	nsubjpass	It,shown
R2834	T11067	T11038	punct	.,transfected
R2835	T10944	T10943	aux	has,shown
R2836	T11069	T11070	nsubj	Antibodies,coimmunoprecipitated
R2837	T11071	T11069	prep	against,Antibodies
R2838	T11072	T11071	pobj	GFP,against
R2839	T10945	T10943	advmod	also,shown
R2840	T11073	T11074	amod	wild,type
R2841	T11074	T11076	compound	type,AQP2
R2842	T10946	T10943	auxpass	been,shown
R2843	T11075	T11074	punct	-,type
R2844	T11076	T11070	dobj	AQP2,coimmunoprecipitated
R2845	T11077	T11078	advmod	when,transfected
R2846	T10947	T10948	mark	that,rescue
R2847	T11078	T11070	advcl	transfected,coimmunoprecipitated
R2848	T11079	T11080	compound	AQP2,GFP
R2849	T10948	T10943	ccomp	rescue,shown
R2850	T11080	T11078	nsubjpass	GFP,transfected
R2851	T11081	T11080	punct	-,GFP
R2852	T11082	T11080	cc	or,GFP
R2853	T10949	T10950	amod	wild,type
R2854	T11083	T11084	compound	AQP2,GFP
R2855	T11084	T11080	conj	GFP,GFP
R2856	T11085	T11084	punct	-,GFP
R2857	T10950	T10952	compound	type,protein
R2858	T11086	T11084	compound	F204V,GFP
R2859	T11087	T11084	punct	-,GFP
R2860	T10951	T10950	punct	-,type
R2861	T11088	T11078	auxpass	was,transfected
R2862	T11089	T11078	advmod	transiently,transfected
R2863	T11090	T11078	punct	", ",transfected
R2864	T10952	T10948	nsubj	protein,rescue
R2865	T11091	T11078	cc	but,transfected
R2866	T11092	T11091	neg	not,but
R2867	T11093	T11094	advmod	when,transfected
R2868	T10953	T10952	compound	AQP2,protein
R2869	T11094	T11078	conj	transfected,transfected
R2870	T11095	T11094	nsubjpass	GFP,transfected
R2871	T10954	T10948	aux	can,rescue
R2872	T11096	T11095	prep	by,GFP
R2873	T10955	T10956	det	a,protein
R2874	T10956	T10948	dobj	protein,rescue
R2875	T10957	T10958	npadvmod	translocation,defective
R2876	T11097	T11096	pobj	itself,by
R2877	T11098	T11094	auxpass	was,transfected
R2878	T11099	T11094	advmod	transiently,transfected
R2879	T11100	T11094	prep	into,transfected
R2880	T11101	T11102	compound	MDCK,cells
R2881	T10958	T10956	amod	defective,protein
R2882	T11102	T11100	pobj	cells,into
R2883	T11103	T11104	advmod	stably,expressing
R2884	T11104	T11102	acl	expressing,cells
R2885	T10959	T10958	punct	-,defective
R2886	T11105	T11106	amod	wild,type
R2887	T11106	T11108	compound	type,AQP2
R2888	T11107	T11106	punct	-,type
R2889	T10960	T10956	compound	mutant,protein
R2890	T11108	T11104	dobj	AQP2,expressing
R2891	T11109	T11110	punct	(,blots
R2892	T10961	T10956	punct	", ",protein
R2893	T11110	T11070	parataxis	blots,coimmunoprecipitated
R2894	T11111	T11110	dep	Figure,blots
R2895	T11112	T11111	nummod	5B,Figure
R2896	T10962	T10963	compound	AQP2,P262L
R2897	T11113	T11110	punct	", ",blots
R2898	T11114	T11110	amod	upper,blots
R2899	T11115	T11110	punct	),blots
R2900	T10963	T10956	appos	P262L,protein
R2901	T11116	T11070	punct	.,coimmunoprecipitated
R2902	T10964	T10963	punct	-,P262L
R2903	T11118	T11119	compound	Western,blot
R2904	T11119	T11120	nsubj	blot,showed
R2905	T10965	T10948	punct	", ",rescue
R2906	T11121	T11119	prep	of,blot
R2907	T11122	T11123	amod	total,membranes
R2908	T11123	T11121	pobj	membranes,of
R2909	T10966	T10967	advmod	when,coexpressed
R2910	T11124	T11125	mark	that,expressed
R2911	T11125	T11120	ccomp	expressed,showed
R2912	T11126	T11127	amod	wild,type
R2913	T10967	T10948	advcl	coexpressed,rescue
R2914	T11127	T11129	compound	type,AQP2
R2915	T11128	T11127	punct	-,type
R2916	T11129	T11125	nsubjpass	AQP2,expressed
R2917	T10968	T10969	det	the,two
R2918	T11130	T11125	auxpass	is,expressed
R2919	T11131	T11125	advmod	equivalently,expressed
R2920	T10969	T10967	nsubjpass	two,coexpressed
R2921	T11132	T11125	prep	in,expressed
R2922	T11133	T11134	det	all,cases
R2923	T11134	T11132	pobj	cases,in
R2924	T11135	T11134	nummod	three,cases
R2925	T10970	T10967	auxpass	are,coexpressed
R2926	T11136	T11137	punct	(,blots
R2927	T11137	T11120	parataxis	blots,showed
R2928	T11138	T11137	dep	Figure,blots
R2929	T11139	T11138	nummod	5B,Figure
R2930	T11140	T11137	punct	", ",blots
R2931	T11141	T11137	amod	lower,blots
R2932	T10971	T10967	prep	in,coexpressed
R2933	T11142	T11137	punct	),blots
R2934	T11143	T11120	punct	.,showed
R2935	T11145	T11146	mark	If,interacting
R2936	T10972	T10973	compound	MDCK,cells
R2937	T10973	T10971	pobj	cells,in
R2938	T11146	T11155	advcl	interacting,is
R2939	T10974	T10975	punct	[,25
R2940	T11147	T11148	amod	wild,type
R2941	T11148	T11150	nmod	type,proteins
R2942	T11149	T11148	punct	-,type
R2943	T11150	T11146	nsubj	proteins,interacting
R2944	T10975	T10943	parataxis	25,shown
R2945	T11151	T11148	cc	and,type
R2946	T11152	T11148	conj	mutant,type
R2947	T11153	T11146	aux	are,interacting
R2948	T10976	T10975	punct	],25
R2949	T11154	T11146	advmod	indeed,interacting
R2950	T11156	T11146	prep	in,interacting
R2951	T10977	T10943	punct	.,shown
R2952	T11157	T11158	det	the,cell
R2953	T11158	T11156	pobj	cell,in
R2954	T11159	T11155	punct	", ",is
R2955	T10979	T10980	prep	In,rescue
R2956	T11160	T11161	det	this,interaction
R2957	T11161	T11155	nsubj	interaction,is
R2958	T11162	T11155	acomp	sufficient,is
R2959	T11163	T11164	aux	to,rescue
R2960	T10981	T10982	det	the,ducts
R2961	T11164	T11162	xcomp	rescue,sufficient
R2962	T11165	T11166	det	the,localization
R2963	T11166	T11164	dobj	localization,rescue
R2964	T10982	T10979	pobj	ducts,In
R2965	T11167	T11166	prep	of,localization
R2966	T11168	T11169	compound	mutant,protein
R2967	T11169	T11167	pobj	protein,of
R2968	T11170	T11155	punct	?,is
R2969	T10983	T10982	amod	collecting,ducts
R2970	T11172	T11173	aux	To,answer
R2971	T11173	T11174	advcl	answer,used
R2972	T10984	T10982	prep	from,ducts
R2973	T11175	T11176	det	this,question
R2974	T11176	T11173	dobj	question,answer
R2975	T10985	T10986	nmod	Aqp2F204V,mice
R2976	T11177	T11174	punct	", ",used
R2977	T11178	T11174	nsubj	we,used
R2978	T10986	T10984	pobj	mice,from
R2979	T11179	T11180	compound	MDCK,cells
R2980	T11180	T11174	dobj	cells,used
R2981	T11181	T11182	advmod	stably,transfected
R2982	T11182	T11180	acl	transfected,cells
R2983	T11183	T11182	prep	with,transfected
R2984	T11184	T11185	amod	wild,type
R2985	T10987	T10985	punct	/,Aqp2F204V
R2986	T11185	T11187	compound	type,AQP2
R2987	T11186	T11185	punct	-,type
R2988	T11187	T11183	pobj	AQP2,with
R2989	T10988	T10985	punct	+,Aqp2F204V
R2990	T11188	T11187	acl	expressing,AQP2
R2991	T11189	T11188	dobj	vector,expressing
R2992	T11190	T11188	cc	or,expressing
R2993	T10989	T10980	punct	", ",rescue
R2994	T11191	T11188	conj	with,expressing
R2995	T11192	T11193	amod	empty,vector
R2996	T11193	T11191	pobj	vector,with
R2997	T10990	T10991	det	the,type
R2998	T11194	T11174	punct	.,used
R2999	T11196	T11197	prep	On,transfected
R3000	T11203	T11197	advmod	transiently,transfected
R3001	T11198	T11196	pobj	top,On
R3002	T11199	T11198	prep	of,top
R3003	T11200	T11199	pobj	these,of
R3004	T11201	T11197	punct	", ",transfected
R3005	T11204	T11205	nmod	AQP2,GFP
R3006	T11202	T11197	nsubj	we,transfected
R3007	T11205	T11207	nmod	GFP,constructs
R3008	T11206	T11205	punct	-,GFP
R3009	T11207	T11197	dobj	constructs,transfected
R3010	T11208	T11205	cc	or,GFP
R3011	T11308	T11306	compound	F204V,GFP
R3012	T11309	T11306	punct	-,GFP
R3013	T11209	T11210	compound	AQP2,GFP
R3014	T11310	T11295	prep	to,localized
R3015	T11311	T11312	det	the,surface
R3016	T11312	T11310	pobj	surface,to
R3017	T11210	T11205	conj	GFP,GFP
R3018	T11313	T11312	amod	apical,surface
R3019	T11314	T11295	prep	to,localized
R3020	T11211	T11210	punct	-,GFP
R3021	T11315	T11316	amod	varying,degrees
R3022	T11316	T11314	pobj	degrees,to
R3023	T11317	T11318	punct	(,images
R3024	T11212	T11210	compound	F204V,GFP
R3025	T11318	T11295	parataxis	images,localized
R3026	T11319	T11318	dep	Figure,images
R3027	T11320	T11319	nummod	5C,Figure
R3028	T11213	T11210	punct	-,GFP
R3029	T11321	T11318	punct	", ",images
R3030	T11322	T11318	amod	lower,images
R3031	T11214	T11207	compound	expression,constructs
R3032	T11323	T11318	amod	right,images
R3033	T11324	T11325	punct	[,i
R3034	T11325	T11318	parataxis	i,images
R3035	T11215	T11197	punct	.,transfected
R3036	T11326	T11327	punct	–,iii
R3037	T11327	T11325	prep	iii,i
R3038	T11217	T11218	compound	AQP2,GFP
R3039	T11328	T11325	punct	],i
R3040	T11329	T11318	punct	),images
R3041	T11330	T11295	punct	.,localized
R3042	T11218	T11220	nsubj	GFP,localized
R3043	T11332	T11333	det	The,images
R3044	T11333	T11336	nsubj	images,shows
R3045	T11219	T11218	punct	-,GFP
R3046	T11334	T11335	amod	lower,right
R3047	T11335	T11333	amod	right,images
R3048	T11221	T11220	prep	to,localized
R3049	T11337	T11333	prep	of,images
R3050	T11338	T11337	pobj	Figure,of
R3051	T11339	T11338	nummod	5C,Figure
R3052	T11222	T11223	det	the,surface
R3053	T11340	T11341	nummod	three,cells
R3054	T11341	T11336	dobj	cells,shows
R3055	T11342	T11341	prep	from,cells
R3056	T11223	T11221	pobj	surface,to
R3057	T11343	T11344	det	a,transfection
R3058	T11344	T11342	pobj	transfection,from
R3059	T11345	T11344	amod	single,transfection
R3060	T11224	T11223	amod	apical,surface
R3061	T11346	T11336	punct	.,shows
R3062	T11348	T11349	det	The,first
R3063	T11225	T11220	prep	upon,localized
R3064	T11349	T11350	nsubj	first,is
R3065	T11226	T11227	compound	forskolin,stimulation
R3066	T11351	T11352	det	a,cell
R3067	T11352	T11350	attr	cell,is
R3068	T11353	T11352	amod	nontransfected,cell
R3069	T11227	T11225	pobj	stimulation,upon
R3070	T11354	T11355	dep	that,shows
R3071	T11355	T11352	relcl	shows,cell
R3072	T11356	T11357	det	the,localization
R3073	T11228	T11229	mark	whether,transfected
R3074	T11357	T11355	dobj	localization,shows
R3075	T11358	T11357	prep	of,localization
R3076	T11359	T11360	det	the,AQP2
R3077	T11229	T11220	advcl	transfected,localized
R3078	T11360	T11358	pobj	AQP2,of
R3079	T11230	T11229	nsubjpass	it,transfected
R3080	T11361	T11362	advmod	stably,expressing
R3081	T11231	T11229	auxpass	was,transfected
R3082	T11362	T11360	amod	expressing,AQP2
R3083	T11363	T11364	amod	wild,type
R3084	T11232	T11229	advmod	transiently,transfected
R3085	T11364	T11360	compound	type,AQP2
R3086	T11365	T11364	punct	-,type
R3087	T11366	T11360	punct	", ",AQP2
R3088	T11367	T11368	dep	which,is
R3089	T11368	T11360	relcl	is,AQP2
R3090	T11233	T11229	prep	into,transfected
R3091	T11369	T11368	acomp	apical,is
R3092	T11370	T11368	prep	upon,is
R3093	T11371	T11372	compound	forskolin,stimulation
R3094	T11234	T11235	npadvmod	vector,only
R3095	T11372	T11370	pobj	stimulation,upon
R3096	T11373	T11350	punct	.,is
R3097	T11235	T11237	amod	only,cells
R3098	T11375	T11376	det	The,two
R3099	T11376	T11378	nsubj	two,show
R3100	T11377	T11376	amod	next,two
R3101	T11236	T11235	punct	-,only
R3102	T11379	T11378	dobj	expression,show
R3103	T11380	T11379	prep	of,expression
R3104	T11237	T11233	pobj	cells,into
R3105	T11381	T11382	preconj	both,AQP2
R3106	T11382	T11380	pobj	AQP2,of
R3107	T11238	T11239	punct	(,images
R3108	T11383	T11382	det	the,AQP2
R3109	T11384	T11382	amod	stable,AQP2
R3110	T11385	T11386	amod	wild,type
R3111	T11239	T11237	parataxis	images,cells
R3112	T11386	T11382	compound	type,AQP2
R3113	T11387	T11386	punct	-,type
R3114	T11388	T11382	cc	and,AQP2
R3115	T11240	T11239	dep	Figure,images
R3116	T11389	T11390	det	the,GFP
R3117	T11390	T11382	conj	GFP,AQP2
R3118	T11391	T11390	amod	transient,GFP
R3119	T11241	T11240	nummod	5C,Figure
R3120	T11392	T11390	compound	AQP2,GFP
R3121	T11393	T11390	punct	-,GFP
R3122	T11394	T11390	compound	F204V,GFP
R3123	T11242	T11239	punct	", ",images
R3124	T11395	T11390	punct	-,GFP
R3125	T11396	T11378	punct	.,show
R3126	T11243	T11239	amod	upper,images
R3127	T11398	T11399	prep	In,was
R3128	T11399	T11411	ccomp	was,were
R3129	T11244	T11239	amod	left,images
R3130	T11400	T11398	pobj	cell,In
R3131	T11401	T11400	punct	(,cell
R3132	T11402	T11400	nummod	ii,cell
R3133	T11403	T11400	punct	),cell
R3134	T11245	T11239	punct	),images
R3135	T11404	T11399	punct	", ",was
R3136	T11405	T11399	nsubj	localization,was
R3137	T11406	T11405	prep	of,localization
R3138	T11407	T11408	amod	wild,type
R3139	T11408	T11410	compound	type,AQP2
R3140	T11409	T11408	punct	-,type
R3141	T11246	T11233	cc	or,into
R3142	T11410	T11406	pobj	AQP2,of
R3143	T11412	T11399	acomp	indistinguishable,was
R3144	T11247	T11233	conj	into,into
R3145	T11248	T11249	amod	wild,type
R3146	T11249	T11251	compound	type,cells
R3147	T11413	T11412	prep	from,indistinguishable
R3148	T11414	T11415	compound	AQP2,GFP
R3149	T11250	T11249	punct	-,type
R3150	T11251	T11247	pobj	cells,into
R3151	T11415	T11413	pobj	GFP,from
R3152	T11416	T11415	punct	-,GFP
R3153	T11252	T11251	compound	AQP2,cells
R3154	T11417	T11415	compound	F204V,GFP
R3155	T11418	T11415	punct	-,GFP
R3156	T11419	T11411	punct	;,were
R3157	T11253	T11254	punct	(,Figure
R3158	T11420	T11411	nsubj	both,were
R3159	T11421	T11411	acomp	apical,were
R3160	T11254	T11251	parataxis	Figure,cells
R3161	T11422	T11411	prep	upon,were
R3162	T11423	T11424	compound	forskolin,stimulation
R3163	T11255	T11254	nummod	5C,Figure
R3164	T11424	T11422	pobj	stimulation,upon
R3165	T11425	T11411	punct	.,were
R3166	T11256	T11254	punct	", ",Figure
R3167	T11427	T11428	mark	Although,was
R3168	T11428	T11431	advcl	was,localized
R3169	T11257	T11258	amod	upper,right
R3170	T11429	T11430	det	the,effect
R3171	T11430	T11428	nsubj	effect,was
R3172	T11258	T11254	amod	right,Figure
R3173	T11432	T11428	acomp	subtle,was
R3174	T11433	T11428	prep	in,was
R3175	T11259	T11254	punct	),Figure
R3176	T11434	T11433	pobj	cell,in
R3177	T11435	T11434	punct	(,cell
R3178	T11436	T11434	nummod	iii,cell
R3179	T11260	T11220	punct	.,localized
R3180	T11437	T11434	punct	),cell
R3181	T11438	T11431	punct	", ",localized
R3182	T11439	T11440	compound	AQP2,GFP
R3183	T11262	T11263	compound	AQP2,GFP
R3184	T11440	T11431	nsubjpass	GFP,localized
R3185	T11441	T11440	punct	-,GFP
R3186	T11442	T11440	compound	F204V,GFP
R3187	T11443	T11440	punct	-,GFP
R3188	T11444	T11431	auxpass	was,localized
R3189	T11445	T11431	advmod	partly,localized
R3190	T11446	T11431	prep	to,localized
R3191	T11263	T11267	nsubj	GFP,showed
R3192	T11447	T11448	det	the,surface
R3193	T11448	T11446	pobj	surface,to
R3194	T11449	T11448	amod	apical,surface
R3195	T11264	T11263	punct	-,GFP
R3196	T11450	T11431	punct	.,localized
R3197	T11452	T11453	advmod	Generally,was
R3198	T11265	T11263	compound	F204V,GFP
R3199	T11454	T11453	punct	", ",was
R3200	T11266	T11263	punct	-,GFP
R3201	T11455	T11456	det	the,localization
R3202	T11456	T11453	nsubj	localization,was
R3203	T11457	T11456	prep	of,localization
R3204	T11268	T11267	punct	", ",showed
R3205	T11458	T11459	compound	AQP2,GFP
R3206	T11459	T11457	pobj	GFP,of
R3207	T11460	T11459	punct	-,GFP
R3208	T11461	T11459	compound	F204V,GFP
R3209	T11269	T11270	advmod	when,expressed
R3210	T11462	T11459	punct	-,GFP
R3211	T11463	T11453	advmod	clearly,was
R3212	T11464	T11465	advmod	more,apical
R3213	T11270	T11267	advcl	expressed,showed
R3214	T11465	T11453	acomp	apical,was
R3215	T11466	T11467	advmod	when,expressed
R3216	T11467	T11453	advcl	expressed,was
R3217	T11271	T11270	prep	by,expressed
R3218	T11468	T11469	amod	wild,type
R3219	T11469	T11471	compound	type,AQP2
R3220	T11470	T11469	punct	-,type
R3221	T11272	T11273	amod	transient,transfection
R3222	T11471	T11467	nsubjpass	AQP2,expressed
R3223	T11472	T11467	auxpass	was,expressed
R3224	T11473	T11467	advmod	also,expressed
R3225	T11273	T11271	pobj	transfection,by
R3226	T11474	T11453	punct	.,was
R3227	T11274	T11270	prep	into,expressed
R3228	T11476	T11477	aux	To,confirm
R3229	T11477	T11478	advcl	confirm,transfected
R3230	T11275	T11276	npadvmod	vector,only
R3231	T11479	T11480	det	these,results
R3232	T11480	T11477	dobj	results,confirm
R3233	T11276	T11277	amod	only,cells
R3234	T11481	T11477	advmod	biochemically,confirm
R3235	T11482	T11478	punct	", ",transfected
R3236	T11483	T11478	nsubj	we,transfected
R3237	T11277	T11274	pobj	cells,into
R3238	T11484	T11485	det	the,lines
R3239	T11485	T11478	dobj	lines,transfected
R3240	T11486	T11485	amod	same,lines
R3241	T11487	T11485	compound	cell,lines
R3242	T11488	T11485	punct	(,lines
R3243	T11278	T11267	punct	", ",showed
R3244	T11489	T11490	amod	wild,type
R3245	T11490	T11492	compound	type,AQP2
R3246	T11491	T11490	punct	-,type
R3247	T11279	T11280	det	a,pattern
R3248	T11492	T11485	appos	AQP2,lines
R3249	T11493	T11492	cc	or,AQP2
R3250	T11494	T11492	conj	vector,AQP2
R3251	T11280	T11267	dobj	pattern,showed
R3252	T11495	T11485	punct	),lines
R3253	T11496	T11478	prep	with,transfected
R3254	T11497	T11498	compound	F204V,GFP
R3255	T11281	T11280	amod	diffuse,pattern
R3256	T11498	T11496	pobj	GFP,with
R3257	T11499	T11498	punct	-,GFP
R3258	T11500	T11478	punct	", ",transfected
R3259	T11282	T11280	amod	cytoplasmic,pattern
R3260	T11501	T11478	conj	biotinylated,transfected
R3261	T11283	T11280	compound	distribution,pattern
R3262	T11502	T11503	compound	surface,proteins
R3263	T11284	T11285	punct	(,left
R3264	T11503	T11501	dobj	proteins,biotinylated
R3265	T11504	T11501	prep	after,biotinylated
R3266	T11505	T11506	compound	forskolin,stimulation
R3267	T11285	T11267	parataxis	left,showed
R3268	T11506	T11504	pobj	stimulation,after
R3269	T11507	T11501	punct	", ",biotinylated
R3270	T11286	T11285	dep	Figure,left
R3271	T11508	T11501	cc	and,biotinylated
R3272	T11509	T11501	conj	precipitated,biotinylated
R3273	T11510	T11511	det	the,proteins
R3274	T11287	T11286	nummod	5C,Figure
R3275	T11511	T11509	dobj	proteins,precipitated
R3276	T11512	T11511	amod	biotinylated,proteins
R3277	T11288	T11285	punct	", ",left
R3278	T11513	T11514	punct	(,Figure
R3279	T11514	T11509	parataxis	Figure,precipitated
R3280	T11515	T11514	nummod	5D,Figure
R3281	T11289	T11285	amod	lower,left
R3282	T11516	T11514	punct	),Figure
R3283	T11517	T11478	punct	.,transfected
R3284	T11290	T11285	punct	),left
R3285	T11291	T11267	punct	.,showed
R3286	T11293	T11294	advmod	When,expressed
R3287	T11519	T11520	compound	AQP2,GFP
R3288	T11520	T11524	nsubjpass	GFP,expressed
R3289	T11294	T11295	advcl	expressed,localized
R3290	T11521	T11520	punct	-,GFP
R3291	T11522	T11520	compound	F204V,GFP
R3292	T11523	T11520	punct	-,GFP
R3293	T11525	T11524	auxpass	is,expressed
R3294	T11296	T11294	prep	in,expressed
R3295	T11526	T11527	advmod	approximately,equally
R3296	T11527	T11524	advmod	equally,expressed
R3297	T11528	T11524	prep	in,expressed
R3298	T11529	T11530	det	both,lines
R3299	T11530	T11528	pobj	lines,in
R3300	T11297	T11298	amod	wild,type
R3301	T11531	T11530	compound	cell,lines
R3302	T11532	T11533	punct	(,cells
R3303	T11533	T11524	parataxis	cells,expressed
R3304	T11298	T11300	compound	type,cells
R3305	T11534	T11533	dep	Figure,cells
R3306	T11535	T11534	nummod	5D,Figure
R3307	T11536	T11533	punct	", ",cells
R3308	T11299	T11298	punct	-,type
R3309	T11537	T11533	amod	total,cells
R3310	T11538	T11533	punct	),cells
R3311	T11300	T11296	pobj	cells,in
R3312	T11539	T11524	punct	", ",expressed
R3313	T11540	T11524	cc	but,expressed
R3314	T11541	T11542	auxpass	is,biotinylated
R3315	T11301	T11300	compound	AQP2,cells
R3316	T11542	T11524	conj	biotinylated,expressed
R3317	T11543	T11544	advmod	only,expressed
R3318	T11544	T11542	ccomp	expressed,biotinylated
R3319	T11302	T11295	punct	", ",localized
R3320	T11545	T11544	advmod	when,expressed
R3321	T11546	T11547	amod	wild,type
R3322	T11547	T11549	compound	type,AQP2
R3323	T11303	T11295	advmod	however,localized
R3324	T11548	T11547	punct	-,type
R3325	T11549	T11544	nsubjpass	AQP2,expressed
R3326	T11550	T11544	auxpass	is,expressed
R3327	T11304	T11295	punct	", ",localized
R3328	T11551	T11544	advmod	also,expressed
R3329	T11552	T11553	punct	(,Figure
R3330	T11553	T11542	parataxis	Figure,biotinylated
R3331	T11305	T11306	compound	AQP2,GFP
R3332	T11554	T11553	nummod	5D,Figure
R3333	T11555	T11553	punct	", ",Figure
R3334	T11556	T11557	npadvmod	surface,biotinylated
R3335	T11306	T11295	nsubj	GFP,localized
R3336	T11557	T11553	amod	biotinylated,Figure
R3337	T11558	T11553	punct	),Figure
R3338	T11559	T11524	punct	.,expressed
R3339	T11307	T11306	punct	-,GFP
R3340	T11561	T11562	mark	Since,are
R3341	T11562	T11567	advcl	are,indicate
R3342	T11563	T11564	advmod	only,proteins
R3343	T11564	T11562	nsubj	proteins,are
R3344	T11565	T11566	compound	cell,surface
R3345	T11566	T11564	compound	surface,proteins
R3346	T11568	T11562	acomp	accessible,are
R3347	T11569	T11568	prep	to,accessible
R3348	T11570	T11569	pobj	biotin,to
R3349	T11571	T11567	punct	", ",indicate
R3350	T11572	T11573	det	these,results
R3351	T11573	T11567	nsubj	results,indicate
R3352	T11574	T11575	mark	that,transported
R3353	T11575	T11567	ccomp	transported,indicate
R3354	T11576	T11577	compound	AQP2,F204V
R3355	T11577	T11575	nsubjpass	F204V,transported
R3356	T11578	T11577	punct	-,F204V
R3357	T11579	T11575	auxpass	is,transported
R3358	T11580	T11575	prep	to,transported
R3359	T11581	T11582	det	the,surface
R3360	T11582	T11580	pobj	surface,to
R3361	T11583	T11582	compound	cell,surface
R3362	T11584	T11585	advmod	when,is
R3363	T11585	T11575	advcl	is,transported
R3364	T11586	T11587	amod	wild,type
R3365	T11587	T11589	compound	type,AQP2
R3366	T11588	T11587	punct	-,type
R3367	T11589	T11585	nsubj	AQP2,is
R3368	T11590	T11585	acomp	present,is
R3369	T11591	T11585	punct	", ",is
R3370	T11592	T11585	cc	but,is
R3371	T11593	T11592	neg	not,but
R3372	T11594	T11585	conj	on,is
R3373	T11595	T11596	poss	its,own
R3374	T11596	T11594	pobj	own,on
R3375	T11597	T11567	punct	.,indicate

2_test

Id	Subject	Object	Predicate	Lexical cue
16121255-15064401-85587788	180-182	15064401	denotes	19
16121255-11567631-85587789	183-185	11567631	denotes	20
16121255-11793119-85587790	3253-3255	11793119	denotes	21
16121255-11035038-85587791	4008-4010	11035038	denotes	18
16121255-9916798-85587792	5845-5847	9916798	denotes	17
16121255-11035038-85587793	6487-6489	11035038	denotes	18
16121255-14593099-85587794	6490-6492	14593099	denotes	22
16121255-14593099-85587795	6926-6928	14593099	denotes	22
16121255-7517548-85587796	9357-9359	7517548	denotes	23
16121255-11929850-85587797	13458-13460	11929850	denotes	24
16121255-14593099-85587798	13507-13509	14593099	denotes	22
16121255-14593099-85587799	14279-14281	14593099	denotes	22
16121255-15509592-85587800	14444-14446	15509592	denotes	25
T47462	180-182	15064401	denotes	19
T2172	183-185	11567631	denotes	20
T493	3253-3255	11793119	denotes	21
T51608	4008-4010	11035038	denotes	18
T17076	5845-5847	9916798	denotes	17
T44574	6487-6489	11035038	denotes	18
T91505	6490-6492	14593099	denotes	22
T46479	6926-6928	14593099	denotes	22
T63600	9357-9359	7517548	denotes	23
T18045	13458-13460	11929850	denotes	24
T35488	13507-13509	14593099	denotes	22
T95687	14279-14281	14593099	denotes	22
T49378	14444-14446	15509592	denotes	25

PMC:1189073 / 6697-25762 JSONTXT

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2_test

PMC:1189073 / 6697-25762 JSON TXT