PMC:1189073 / 36903-37035 JSONTXT

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    craft-ca-core-ex-dev

    {"project":"craft-ca-core-ex-dev","denotations":[{"id":"T18750","span":{"begin":50,"end":58},"obj":"CHEBI_PR_EXT:protein"},{"id":"T18751","span":{"begin":79,"end":85},"obj":"CHEBI_CHMO_EXT:buffer_solution"},{"id":"T18752","span":{"begin":125,"end":131},"obj":"CHEBI_CHMO_EXT:buffer_solution"}],"text":" Following overnight incubation, the precipitated proteins were washed in RIPA buffer and finally boiled in 50 μl of Laemmli buffer."}

    craft-sa-dev

    {"project":"craft-sa-dev","denotations":[{"id":"T18947","span":{"begin":0,"end":132},"obj":"sentence"},{"id":"T18948","span":{"begin":1,"end":10},"obj":"VBG"},{"id":"T18949","span":{"begin":64,"end":70},"obj":"VBN"},{"id":"T18950","span":{"begin":11,"end":20},"obj":"JJ"},{"id":"T18951","span":{"begin":21,"end":31},"obj":"NN"},{"id":"T18952","span":{"begin":31,"end":33},"obj":","},{"id":"T18953","span":{"begin":33,"end":36},"obj":"DT"},{"id":"T18954","span":{"begin":50,"end":58},"obj":"NN"},{"id":"T18955","span":{"begin":37,"end":49},"obj":"VBN"},{"id":"T18956","span":{"begin":59,"end":63},"obj":"VBD"},{"id":"T18957","span":{"begin":71,"end":73},"obj":"IN"},{"id":"T18958","span":{"begin":74,"end":78},"obj":"NN"},{"id":"T18959","span":{"begin":79,"end":85},"obj":"NN"},{"id":"T18960","span":{"begin":86,"end":89},"obj":"CC"},{"id":"T18961","span":{"begin":90,"end":97},"obj":"RB"},{"id":"T18962","span":{"begin":98,"end":104},"obj":"VBN"},{"id":"T18963","span":{"begin":105,"end":107},"obj":"IN"},{"id":"T18964","span":{"begin":108,"end":110},"obj":"CD"},{"id":"T18965","span":{"begin":111,"end":113},"obj":"NN"},{"id":"T18966","span":{"begin":114,"end":116},"obj":"IN"},{"id":"T18967","span":{"begin":117,"end":124},"obj":"NNP"},{"id":"T18968","span":{"begin":125,"end":131},"obj":"NN"},{"id":"T18969","span":{"begin":131,"end":132},"obj":"."}],"relations":[{"id":"R5443","pred":"prep","subj":"T18948","obj":"T18949"},{"id":"R5444","pred":"amod","subj":"T18950","obj":"T18951"},{"id":"R5445","pred":"pobj","subj":"T18951","obj":"T18948"},{"id":"R5446","pred":"punct","subj":"T18952","obj":"T18949"},{"id":"R5447","pred":"det","subj":"T18953","obj":"T18954"},{"id":"R5448","pred":"nsubjpass","subj":"T18954","obj":"T18949"},{"id":"R5449","pred":"amod","subj":"T18955","obj":"T18954"},{"id":"R5450","pred":"auxpass","subj":"T18956","obj":"T18949"},{"id":"R5451","pred":"prep","subj":"T18957","obj":"T18949"},{"id":"R5452","pred":"compound","subj":"T18958","obj":"T18959"},{"id":"R5453","pred":"pobj","subj":"T18959","obj":"T18957"},{"id":"R5454","pred":"cc","subj":"T18960","obj":"T18949"},{"id":"R5455","pred":"advmod","subj":"T18961","obj":"T18962"},{"id":"R5456","pred":"conj","subj":"T18962","obj":"T18949"},{"id":"R5457","pred":"prep","subj":"T18963","obj":"T18962"},{"id":"R5458","pred":"nummod","subj":"T18964","obj":"T18965"},{"id":"R5459","pred":"pobj","subj":"T18965","obj":"T18963"},{"id":"R5460","pred":"prep","subj":"T18966","obj":"T18965"},{"id":"R5461","pred":"compound","subj":"T18967","obj":"T18968"},{"id":"R5462","pred":"pobj","subj":"T18968","obj":"T18966"},{"id":"R5463","pred":"punct","subj":"T18969","obj":"T18949"}],"text":" Following overnight incubation, the precipitated proteins were washed in RIPA buffer and finally boiled in 50 μl of Laemmli buffer."}