PMC:1189073 / 36903-37035 JSON TXT

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Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id	Subject	Object	Predicate	Lexical cue
T18750	50-58	CHEBI_PR_EXT:protein	denotes	proteins
T18751	79-85	CHEBI_CHMO_EXT:buffer_solution	denotes	buffer
T18752	125-131	CHEBI_CHMO_EXT:buffer_solution	denotes	buffer

Id	Subject	Object	Predicate	Lexical cue
T18947	0-132	sentence	denotes	Following overnight incubation, the precipitated proteins were washed in RIPA buffer and finally boiled in 50 μl of Laemmli buffer.
T18948	1-10	VBG	denotes	Following
T18949	64-70	VBN	denotes	washed
T18950	11-20	JJ	denotes	overnight
T18951	21-31	NN	denotes	incubation
T18952	31-33	,	denotes	,
T18953	33-36	DT	denotes	the
T18954	50-58	NN	denotes	proteins
T18955	37-49	VBN	denotes	precipitated
T18956	59-63	VBD	denotes	were
T18957	71-73	IN	denotes	in
T18958	74-78	NN	denotes	RIPA
T18959	79-85	NN	denotes	buffer
T18960	86-89	CC	denotes	and
T18961	90-97	RB	denotes	finally
T18962	98-104	VBN	denotes	boiled
T18963	105-107	IN	denotes	in
T18964	108-110	CD	denotes	50
T18965	111-113	NN	denotes	μl
T18966	114-116	IN	denotes	of
T18967	117-124	NNP	denotes	Laemmli
T18968	125-131	NN	denotes	buffer
T18969	131-132	.	denotes	.
R5443	T18948	T18949	prep	Following,washed
R5444	T18950	T18951	amod	overnight,incubation
R5445	T18951	T18948	pobj	incubation,Following
R5446	T18952	T18949	punct	", ",washed
R5447	T18953	T18954	det	the,proteins
R5448	T18954	T18949	nsubjpass	proteins,washed
R5449	T18955	T18954	amod	precipitated,proteins
R5450	T18956	T18949	auxpass	were,washed
R5451	T18957	T18949	prep	in,washed
R5452	T18958	T18959	compound	RIPA,buffer
R5453	T18959	T18957	pobj	buffer,in
R5454	T18960	T18949	cc	and,washed
R5455	T18961	T18962	advmod	finally,boiled
R5456	T18962	T18949	conj	boiled,washed
R5457	T18963	T18962	prep	in,boiled
R5458	T18964	T18965	nummod	50,μl
R5459	T18965	T18963	pobj	μl,in
R5460	T18966	T18965	prep	of,μl
R5461	T18967	T18968	compound	Laemmli,buffer
R5462	T18968	T18966	pobj	buffer,of
R5463	T18969	T18949	punct	.,washed