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PMC:1189073 / 32205-33053 JSONTXT

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craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T15876 13-28 SO_EXT:coding_sequence denotes coding sequence
T15877 32-37 NCBITaxon:10088 denotes mouse
T15878 38-42 PR_EXT:000004182 denotes AQP2
T15879 57-62 SO_EXT:sequence_cloned_entity denotes clone
T15880 97-104 SO_EXT:0000155 denotes plasmid
T15881 129-136 GO:0006266 denotes ligated
T15882 212-220 SO_EXT:sequence_alteration_entity_or_process denotes mutation
T15883 244-252 GO_EXT:biological_direction_or_guidance denotes directed
T15884 253-264 SO_EXT:sequence_alteration_process denotes mutagenesis
T15885 332-347 CHEBI_SO_EXT:oligonucleotide denotes oligonucleotide
T15886 391-400 SO:0000077 denotes antisense
T15887 401-416 CHEBI_SO_EXT:oligonucleotide denotes oligonucleotide
T15888 472-479 SO_EXT:sequence_fusion_entity_or_process denotes fusions
T15889 483-487 PR_EXT:000004182 denotes AQP2
T15890 505-509 PR_EXT:000004182 denotes AQP2
T15891 510-519 SO_EXT:engineered_biological_sequence denotes construct
T15892 556-563 SO_EXT:0000112 denotes primers
T15893 625-635 SO_EXT:0000319 denotes stop codon
T15894 639-643 PR_EXT:000004182 denotes AQP2
T15895 684-689 PR_EXT:P23940 denotes BamHI
T15896 694-701 GO:0006266 denotes ligated
T15897 782-790 SO_EXT:sequence_alteration_entity_or_process denotes mutation
T15898 821-830 CHEBI_EXT:25435 denotes mutagenic
T15899 831-847 CHEBI_SO_EXT:oligonucleotide denotes oligonucleotides

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T15862 32-37 NCBITaxon:10088 denotes mouse
T15863 38-42 PR:000004182 denotes AQP2
T15864 97-104 SO:0000155 denotes plasmid
T15865 129-136 GO:0006266 denotes ligated
T15866 391-400 SO:0000077 denotes antisense
T15867 483-487 PR:000004182 denotes AQP2
T15868 505-509 PR:000004182 denotes AQP2
T15869 556-563 SO:0000112 denotes primers
T15870 625-635 SO:0000319 denotes stop codon
T15871 639-643 PR:000004182 denotes AQP2
T15872 684-689 PR:P23940 denotes BamHI
T15873 694-701 GO:0006266 denotes ligated
T15874 821-830 CHEBI:25435 denotes mutagenic

craft-sa-dev

Id Subject Object Predicate Lexical cue
T15903 0-3 DT denotes The
T15904 20-28 NN denotes sequence
T15905 4-12 JJ denotes complete
T15906 13-19 NN denotes coding
T15907 67-75 VBN denotes digested
T15908 29-31 IN denotes of
T15909 32-37 NN denotes mouse
T15910 38-42 NN denotes AQP2
T15911 43-47 IN denotes from
T15912 48-50 DT denotes an
T15913 57-62 NN denotes clone
T15914 51-56 NN denotes IMAGE
T15915 63-66 VBD denotes was
T15916 76-80 IN denotes from
T15917 81-84 DT denotes the
T15918 97-104 NN denotes plasmid
T15919 85-96 NN denotes pCMV⋅SPORT6
T15920 105-109 IN denotes with
T15921 110-115 NN denotes EcoRI
T15922 116-119 CC denotes and
T15923 120-124 NN denotes NotI
T15924 125-128 CC denotes and
T15925 129-136 VBN denotes ligated
T15926 137-141 IN denotes into
T15927 142-150 NN denotes pcDNA3.1
T15928 151-152 -LRB- denotes (
T15929 152-162 NNP denotes Invitrogen
T15930 162-164 , denotes ,
T15931 164-172 NNP denotes Carlsbad
T15932 172-174 , denotes ,
T15933 174-184 NNP denotes California
T15934 184-186 , denotes ,
T15935 186-192 NNP denotes United
T15936 193-199 NNP denotes States
T15937 199-200 -RRB- denotes )
T15938 200-201 . denotes .
T15939 201-455 sentence denotes The F204V mutation was introduced by site-directed mutagenesis (Stratagene, La Jolla, California, United States), using the sense oligonucleotide 5′-GATGATCACTGGGTCGTCTGGATCGGACCCC-3′, and antisense oligonucleotide 5′-GGGGTCCGATCCAGACGACCCAGTGATCATC-3′.
T15940 202-205 DT denotes The
T15941 212-220 NN denotes mutation
T15942 206-211 NN denotes F204V
T15943 225-235 VBN denotes introduced
T15944 221-224 VBD denotes was
T15945 236-238 IN denotes by
T15946 239-243 NN denotes site
T15947 244-252 JJ denotes directed
T15948 243-244 HYPH denotes -
T15949 253-264 NN denotes mutagenesis
T15950 265-266 -LRB- denotes (
T15951 266-276 NNP denotes Stratagene
T15952 276-278 , denotes ,
T15953 278-280 NNP denotes La
T15954 281-286 NNP denotes Jolla
T15955 286-288 , denotes ,
T15956 288-298 NNP denotes California
T15957 298-300 , denotes ,
T15958 300-306 NNP denotes United
T15959 307-313 NNP denotes States
T15960 313-314 -RRB- denotes )
T15961 314-316 , denotes ,
T15962 316-321 VBG denotes using
T15963 322-325 DT denotes the
T15964 332-347 NN denotes oligonucleotide
T15965 326-331 NN denotes sense
T15966 348-349 CD denotes 5
T15967 351-382 NN denotes GATGATCACTGGGTCGTCTGGATCGGACCCC
T15968 349-350 SYM denotes ′
T15969 350-351 HYPH denotes -
T15970 382-383 HYPH denotes -
T15971 383-384 CD denotes 3
T15972 384-385 SYM denotes ′
T15973 385-387 , denotes ,
T15974 387-390 CC denotes and
T15975 391-400 JJ denotes antisense
T15976 401-416 NN denotes oligonucleotide
T15977 417-418 CD denotes 5
T15978 420-451 NN denotes GGGGTCCGATCCAGACGACCCAGTGATCATC
T15979 418-419 SYM denotes ′
T15980 419-420 HYPH denotes -
T15981 451-452 HYPH denotes -
T15982 452-453 CD denotes 3
T15983 453-454 SYM denotes ′
T15984 454-455 . denotes .
T15985 455-644 sentence denotes To generate GFP fusions of AQP2, the pCMV⋅SPORT6 AQP2 construct was used in a PCR reaction with the primers Sp6 and 5′-GACTGGATCCCGGCCTTGCTGCCGCGCGGCAG-3′ to remove the stop codon of AQP2.
T15986 456-458 TO denotes To
T15987 459-467 VB denotes generate
T15988 524-528 VBN denotes used
T15989 468-471 NN denotes GFP
T15990 472-479 NNS denotes fusions
T15991 480-482 IN denotes of
T15992 483-487 NN denotes AQP2
T15993 487-489 , denotes ,
T15994 489-492 DT denotes the
T15995 510-519 NN denotes construct
T15996 493-504 NN denotes pCMV⋅SPORT6
T15997 505-509 NN denotes AQP2
T15998 520-523 VBD denotes was
T15999 529-531 IN denotes in
T16000 532-533 DT denotes a
T16001 538-546 NN denotes reaction
T16002 534-537 NN denotes PCR
T16003 547-551 IN denotes with
T16004 552-555 DT denotes the
T16005 564-567 NN denotes Sp6
T16006 556-563 NNS denotes primers
T16007 568-571 CC denotes and
T16008 572-573 CD denotes 5
T16009 575-607 NN denotes GACTGGATCCCGGCCTTGCTGCCGCGCGGCAG
T16010 573-574 SYM denotes ′
T16011 574-575 HYPH denotes -
T16012 607-608 HYPH denotes -
T16013 608-609 CD denotes 3
T16014 609-610 SYM denotes ′
T16015 611-613 TO denotes to
T16016 614-620 VB denotes remove
T16017 621-624 DT denotes the
T16018 630-635 NN denotes codon
T16019 625-629 NN denotes stop
T16020 636-638 IN denotes of
T16021 639-643 NN denotes AQP2
T16022 643-644 . denotes .
T16023 644-771 sentence denotes The product was digested with KpnI and BamHI and ligated into pEGFP-N2 (BD Biosciences, San Diego, California, United States).
T16024 645-648 DT denotes The
T16025 649-656 NN denotes product
T16026 661-669 VBN denotes digested
T16027 657-660 VBD denotes was
T16028 670-674 IN denotes with
T16029 675-679 NN denotes KpnI
T16030 680-683 CC denotes and
T16031 684-689 NN denotes BamHI
T16032 690-693 CC denotes and
T16033 694-701 VBN denotes ligated
T16034 702-706 IN denotes into
T16035 707-712 NN denotes pEGFP
T16036 713-715 NN denotes N2
T16037 712-713 HYPH denotes -
T16038 716-717 -LRB- denotes (
T16039 720-731 NNP denotes Biosciences
T16040 717-719 NN denotes BD
T16041 731-733 , denotes ,
T16042 733-736 NNP denotes San
T16043 737-742 NNP denotes Diego
T16044 742-744 , denotes ,
T16045 744-754 NNP denotes California
T16046 754-756 , denotes ,
T16047 756-762 NNP denotes United
T16048 763-769 NNP denotes States
T16049 769-770 -RRB- denotes )
T16050 770-771 . denotes .
T16051 771-848 sentence denotes The F204V mutation was introduced using the same mutagenic oligonucleotides.
T16052 772-775 DT denotes The
T16053 782-790 NN denotes mutation
T16054 776-781 NN denotes F204V
T16055 795-805 VBN denotes introduced
T16056 791-794 VBD denotes was
T16057 806-811 VBG denotes using
T16058 812-815 DT denotes the
T16059 831-847 NNS denotes oligonucleotides
T16060 816-820 JJ denotes same
T16061 821-830 JJ denotes mutagenic
T16062 847-848 . denotes .
R4549 T15903 T15904 det The,sequence
R4550 T15904 T15907 nsubjpass sequence,digested
R4551 T15905 T15904 amod complete,sequence
R4552 T15906 T15904 compound coding,sequence
R4553 T15908 T15904 prep of,sequence
R4554 T15909 T15910 compound mouse,AQP2
R4555 T15910 T15908 pobj AQP2,of
R4556 T15911 T15904 prep from,sequence
R4557 T15912 T15913 det an,clone
R4558 T15913 T15911 pobj clone,from
R4559 T15914 T15913 compound IMAGE,clone
R4560 T15915 T15907 auxpass was,digested
R4561 T15916 T15907 prep from,digested
R4562 T15917 T15918 det the,plasmid
R4563 T15918 T15916 pobj plasmid,from
R4564 T15919 T15918 compound pCMV⋅SPORT6,plasmid
R4565 T15920 T15907 prep with,digested
R4566 T15921 T15920 pobj EcoRI,with
R4567 T15922 T15921 cc and,EcoRI
R4568 T15923 T15921 conj NotI,EcoRI
R4569 T15924 T15907 cc and,digested
R4570 T15925 T15907 conj ligated,digested
R4571 T15926 T15925 prep into,ligated
R4572 T15927 T15926 pobj pcDNA3.1,into
R4573 T15928 T15929 punct (,Invitrogen
R4574 T15929 T15927 parataxis Invitrogen,pcDNA3.1
R4575 T15930 T15929 punct ", ",Invitrogen
R4576 T15931 T15929 npadvmod Carlsbad,Invitrogen
R4577 T15932 T15929 punct ", ",Invitrogen
R4578 T15933 T15929 npadvmod California,Invitrogen
R4579 T15934 T15929 punct ", ",Invitrogen
R4580 T15935 T15936 compound United,States
R4581 T15936 T15929 npadvmod States,Invitrogen
R4582 T15937 T15929 punct ),Invitrogen
R4583 T15938 T15907 punct .,digested
R4584 T15940 T15941 det The,mutation
R4585 T15941 T15943 nsubjpass mutation,introduced
R4586 T15942 T15941 compound F204V,mutation
R4587 T15944 T15943 auxpass was,introduced
R4588 T15945 T15943 prep by,introduced
R4589 T15946 T15947 npadvmod site,directed
R4590 T15947 T15949 amod directed,mutagenesis
R4591 T15948 T15947 punct -,directed
R4592 T15949 T15945 pobj mutagenesis,by
R4593 T15950 T15951 punct (,Stratagene
R4594 T15951 T15949 parataxis Stratagene,mutagenesis
R4595 T15952 T15951 punct ", ",Stratagene
R4596 T15953 T15954 compound La,Jolla
R4597 T15954 T15951 npadvmod Jolla,Stratagene
R4598 T15955 T15951 punct ", ",Stratagene
R4599 T15956 T15951 npadvmod California,Stratagene
R4600 T15957 T15951 punct ", ",Stratagene
R4601 T15958 T15959 compound United,States
R4602 T15959 T15951 npadvmod States,Stratagene
R4603 T15960 T15951 punct ),Stratagene
R4604 T15961 T15943 punct ", ",introduced
R4605 T15962 T15943 advcl using,introduced
R4606 T15963 T15964 det the,oligonucleotide
R4607 T15964 T15962 dobj oligonucleotide,using
R4608 T15965 T15964 compound sense,oligonucleotide
R4609 T15966 T15967 nummod 5,GATGATCACTGGGTCGTCTGGATCGGACCCC
R4610 T15967 T15964 appos GATGATCACTGGGTCGTCTGGATCGGACCCC,oligonucleotide
R4611 T15968 T15966 punct ′,5
R4612 T15969 T15967 punct -,GATGATCACTGGGTCGTCTGGATCGGACCCC
R4613 T15970 T15967 punct -,GATGATCACTGGGTCGTCTGGATCGGACCCC
R4614 T15971 T15967 nummod 3,GATGATCACTGGGTCGTCTGGATCGGACCCC
R4615 T15972 T15967 punct ′,GATGATCACTGGGTCGTCTGGATCGGACCCC
R4616 T15973 T15964 punct ", ",oligonucleotide
R4617 T15974 T15964 cc and,oligonucleotide
R4618 T15975 T15976 amod antisense,oligonucleotide
R4619 T15976 T15964 conj oligonucleotide,oligonucleotide
R4620 T15977 T15978 nummod 5,GGGGTCCGATCCAGACGACCCAGTGATCATC
R4621 T15978 T15976 appos GGGGTCCGATCCAGACGACCCAGTGATCATC,oligonucleotide
R4622 T15979 T15977 punct ′,5
R4623 T15980 T15978 punct -,GGGGTCCGATCCAGACGACCCAGTGATCATC
R4624 T15981 T15978 punct -,GGGGTCCGATCCAGACGACCCAGTGATCATC
R4625 T15982 T15978 nummod 3,GGGGTCCGATCCAGACGACCCAGTGATCATC
R4626 T15983 T15978 punct ′,GGGGTCCGATCCAGACGACCCAGTGATCATC
R4627 T15984 T15943 punct .,introduced
R4628 T15986 T15987 aux To,generate
R4629 T15987 T15988 advcl generate,used
R4630 T15989 T15990 compound GFP,fusions
R4631 T15990 T15987 dobj fusions,generate
R4632 T15991 T15990 prep of,fusions
R4633 T15992 T15991 pobj AQP2,of
R4634 T15993 T15988 punct ", ",used
R4635 T15994 T15995 det the,construct
R4636 T15995 T15988 nsubjpass construct,used
R4637 T15996 T15995 compound pCMV⋅SPORT6,construct
R4638 T15997 T15995 compound AQP2,construct
R4639 T15998 T15988 auxpass was,used
R4640 T15999 T15988 prep in,used
R4641 T16000 T16001 det a,reaction
R4642 T16001 T15999 pobj reaction,in
R4643 T16002 T16001 compound PCR,reaction
R4644 T16003 T16001 prep with,reaction
R4645 T16004 T16005 det the,Sp6
R4646 T16005 T16003 pobj Sp6,with
R4647 T16006 T16005 compound primers,Sp6
R4648 T16007 T16005 cc and,Sp6
R4649 T16008 T16009 nummod 5,GACTGGATCCCGGCCTTGCTGCCGCGCGGCAG
R4650 T16009 T16005 conj GACTGGATCCCGGCCTTGCTGCCGCGCGGCAG,Sp6
R4651 T16010 T16008 punct ′,5
R4652 T16011 T16009 punct -,GACTGGATCCCGGCCTTGCTGCCGCGCGGCAG
R4653 T16012 T16009 punct -,GACTGGATCCCGGCCTTGCTGCCGCGCGGCAG
R4654 T16013 T16009 nummod 3,GACTGGATCCCGGCCTTGCTGCCGCGCGGCAG
R4655 T16014 T16009 punct ′,GACTGGATCCCGGCCTTGCTGCCGCGCGGCAG
R4656 T16015 T16016 aux to,remove
R4657 T16016 T15988 advcl remove,used
R4658 T16017 T16018 det the,codon
R4659 T16018 T16016 dobj codon,remove
R4660 T16019 T16018 compound stop,codon
R4661 T16020 T16018 prep of,codon
R4662 T16021 T16020 pobj AQP2,of
R4663 T16022 T15988 punct .,used
R4664 T16024 T16025 det The,product
R4665 T16025 T16026 nsubjpass product,digested
R4666 T16027 T16026 auxpass was,digested
R4667 T16028 T16026 prep with,digested
R4668 T16029 T16028 pobj KpnI,with
R4669 T16030 T16029 cc and,KpnI
R4670 T16031 T16029 conj BamHI,KpnI
R4671 T16032 T16026 cc and,digested
R4672 T16033 T16026 conj ligated,digested
R4673 T16034 T16033 prep into,ligated
R4674 T16035 T16036 compound pEGFP,N2
R4675 T16036 T16034 pobj N2,into
R4676 T16037 T16036 punct -,N2
R4677 T16038 T16039 punct (,Biosciences
R4678 T16039 T16036 parataxis Biosciences,N2
R4679 T16040 T16039 compound BD,Biosciences
R4680 T16041 T16039 punct ", ",Biosciences
R4681 T16042 T16043 compound San,Diego
R4682 T16043 T16039 npadvmod Diego,Biosciences
R4683 T16044 T16039 punct ", ",Biosciences
R4684 T16045 T16039 npadvmod California,Biosciences
R4685 T16046 T16039 punct ", ",Biosciences
R4686 T16047 T16048 compound United,States
R4687 T16048 T16039 npadvmod States,Biosciences
R4688 T16049 T16039 punct ),Biosciences
R4689 T16050 T16026 punct .,digested
R4690 T16052 T16053 det The,mutation
R4691 T16053 T16055 nsubjpass mutation,introduced
R4692 T16054 T16053 compound F204V,mutation
R4693 T16056 T16055 auxpass was,introduced
R4694 T16057 T16055 advcl using,introduced
R4695 T16058 T16059 det the,oligonucleotides
R4696 T16059 T16057 dobj oligonucleotides,using
R4697 T16060 T16059 amod same,oligonucleotides
R4698 T16061 T16059 amod mutagenic,oligonucleotides
R4699 T16062 T16055 punct .,introduced