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protocol was adapted from one used by Raviola [64] with some modifications derived from Carter-Dawson and Lavail [26]. Four adult wild-type and four mutant animals were deeply anesthetized by intraperitoneal injection of Avertin and the eyes were then removed. The cornea was gently punctured with sharp forceps and excised with iridectomy scissors. The eye was then transferred to a solution of 2% paraformaldehyde/2% glutaraldehyde in cacodylate buffer (0.1 M cacodylic acid; 0.1% calcium chloride). The lens was gently removed and the eyecup allowed to fix for 2 h at room temperature. The fixed eye was then placed on dental wax and sectioned in the midline with a fresh razor blade (half of the retinas were sectioned along the D-V axis and half along the nasal-temporal axis). The retinas (and attached retinal pigment epithelium) were carefully dissected away from the sclera, which was discarded."}

    craft-ca-core-ex-dev

    {"project":"craft-ca-core-ex-dev","denotations":[{"id":"T13421","span":{"begin":135,"end":144},"obj":"SO_EXT:wild_type_entity_or_quality"},{"id":"T13422","span":{"begin":154,"end":160},"obj":"SO_EXT:sequence_altered_entity_or_alteration_process"},{"id":"T13423","span":{"begin":161,"end":168},"obj":"NCBITaxon:33208"},{"id":"T13424","span":{"begin":181,"end":193},"obj":"CHEBI_EXT:anaesthetic_process"},{"id":"T13425","span":{"begin":202,"end":212},"obj":"UBERON:0001179"},{"id":"T13426","span":{"begin":242,"end":246},"obj":"UBERON:0000970"},{"id":"T13427","span":{"begin":270,"end":276},"obj":"UBERON:0000964"},{"id":"T13428","span":{"begin":359,"end":362},"obj":"UBERON:0000970"},{"id":"T13429","span":{"begin":372,"end":383},"obj":"GO_EXT:biological_movement_or_translocation_process"},{"id":"T13430","span":{"begin":389,"end":397},"obj":"CHEBI:75958"},{"id":"T13431","span":{"begin":404,"end":420},"obj":"CHEBI_EXT:paraformaldehye"},{"id":"T13432","span":{"begin":424,"end":438},"obj":"CHEBI:64276"},{"id":"T13433","span":{"begin":442,"end":452},"obj":"CHEBI:16223"},{"id":"T13434","span":{"begin":453,"end":459},"obj":"CHEBI_CHMO_EXT:buffer_solution"},{"id":"T13435","span":{"begin":467,"end":481},"obj":"CHEBI:48765"},{"id":"T13436","span":{"begin":488,"end":504},"obj":"CHEBI:3312"},{"id":"T13437","span":{"begin":511,"end":515},"obj":"UBERON_EXT:lens"},{"id":"T13438","span":{"begin":543,"end":549},"obj":"UBERON:0003072"},{"id":"T13439","span":{"begin":604,"end":607},"obj":"UBERON:0000970"},{"id":"T13440","span":{"begin":627,"end":633},"obj":"UBERON:0001091"},{"id":"T13441","span":{"begin":634,"end":637},"obj":"CHEBI:73702"},{"id":"T13442","span":{"begin":705,"end":712},"obj":"UBERON:0000966"},{"id":"T13443","span":{"begin":766,"end":771},"obj":"UBERON:0000004"},{"id":"T13444","span":{"begin":792,"end":799},"obj":"UBERON:0000966"},{"id":"T13445","span":{"begin":814,"end":840},"obj":"UBERON:0001782"},{"id":"T13446","span":{"begin":822,"end":829},"obj":"CHEBI_EXT:26130"},{"id":"T13447","span":{"begin":881,"end":887},"obj":"UBERON:0001773"}],"text":"This protocol was adapted from one used by Raviola [64] with some modifications derived from Carter-Dawson and Lavail [26]. Four adult wild-type and four mutant animals were deeply anesthetized by intraperitoneal injection of Avertin and the eyes were then removed. The cornea was gently punctured with sharp forceps and excised with iridectomy scissors. The eye was then transferred to a solution of 2% paraformaldehyde/2% glutaraldehyde in cacodylate buffer (0.1 M cacodylic acid; 0.1% calcium chloride). The lens was gently removed and the eyecup allowed to fix for 2 h at room temperature. The fixed eye was then placed on dental wax and sectioned in the midline with a fresh razor blade (half of the retinas were sectioned along the D-V axis and half along the nasal-temporal axis). The retinas (and attached retinal pigment epithelium) were carefully dissected away from the sclera, which was discarded."}

    craft-ca-core-dev

    {"project":"craft-ca-core-dev","denotations":[{"id":"T13357","span":{"begin":161,"end":168},"obj":"NCBITaxon:33208"},{"id":"T13358","span":{"begin":202,"end":212},"obj":"UBERON:0001179"},{"id":"T13359","span":{"begin":242,"end":246},"obj":"UBERON:0000970"},{"id":"T13360","span":{"begin":270,"end":276},"obj":"UBERON:0000964"},{"id":"T13361","span":{"begin":359,"end":362},"obj":"UBERON:0000970"},{"id":"T13362","span":{"begin":389,"end":397},"obj":"CHEBI:75958"},{"id":"T13363","span":{"begin":424,"end":438},"obj":"CHEBI:64276"},{"id":"T13364","span":{"begin":442,"end":452},"obj":"CHEBI:16223"},{"id":"T13365","span":{"begin":467,"end":481},"obj":"CHEBI:48765"},{"id":"T13366","span":{"begin":488,"end":504},"obj":"CHEBI:3312"},{"id":"T13367","span":{"begin":543,"end":549},"obj":"UBERON:0003072"},{"id":"T13368","span":{"begin":604,"end":607},"obj":"UBERON:0000970"},{"id":"T13369","span":{"begin":627,"end":633},"obj":"UBERON:0001091"},{"id":"T13370","span":{"begin":634,"end":637},"obj":"CHEBI:73702"},{"id":"T13371","span":{"begin":705,"end":712},"obj":"UBERON:0000966"},{"id":"T13372","span":{"begin":766,"end":771},"obj":"UBERON:0000004"},{"id":"T13373","span":{"begin":792,"end":799},"obj":"UBERON:0000966"},{"id":"T13374","span":{"begin":814,"end":840},"obj":"UBERON:0001782"},{"id":"T13375","span":{"begin":822,"end":829},"obj":"CHEBI:26130"},{"id":"T13376","span":{"begin":881,"end":887},"obj":"UBERON:0001773"}],"text":"This protocol was adapted from one used by Raviola [64] with some modifications derived from Carter-Dawson and Lavail [26]. Four adult wild-type and four mutant animals were deeply anesthetized by intraperitoneal injection of Avertin and the eyes were then removed. The cornea was gently punctured with sharp forceps and excised with iridectomy scissors. The eye was then transferred to a solution of 2% paraformaldehyde/2% glutaraldehyde in cacodylate buffer (0.1 M cacodylic acid; 0.1% calcium chloride). The lens was gently removed and the eyecup allowed to fix for 2 h at room temperature. The fixed eye was then placed on dental wax and sectioned in the midline with a fresh razor blade (half of the retinas were sectioned along the D-V axis and half along the nasal-temporal axis). The retinas (and attached retinal pigment epithelium) were carefully dissected away from the sclera, which was discarded."}

    2_test

    {"project":"2_test","denotations":[{"id":"16110338-15676071-85798578","span":{"begin":52,"end":54},"obj":"15676071"},{"id":"16110338-500858-85798579","span":{"begin":119,"end":121},"obj":"500858"},{"id":"T90420","span":{"begin":52,"end":54},"obj":"15676071"},{"id":"T88610","span":{"begin":119,"end":121},"obj":"500858"}],"text":"This protocol was adapted from one used by Raviola [64] with some modifications derived from Carter-Dawson and Lavail [26]. Four adult wild-type and four mutant animals were deeply anesthetized by intraperitoneal injection of Avertin and the eyes were then removed. The cornea was gently punctured with sharp forceps and excised with iridectomy scissors. The eye was then transferred to a solution of 2% paraformaldehyde/2% glutaraldehyde in cacodylate buffer (0.1 M cacodylic acid; 0.1% calcium chloride). The lens was gently removed and the eyecup allowed to fix for 2 h at room temperature. The fixed eye was then placed on dental wax and sectioned in the midline with a fresh razor blade (half of the retinas were sectioned along the D-V axis and half along the nasal-temporal axis). The retinas (and attached retinal pigment epithelium) were carefully dissected away from the sclera, which was discarded."}