PMC:1183529 / 29779-30609 JSONTXT

{"project":"craft-ca-core-dev","denotations":[{"id":"T15210","span":{"begin":11,"end":15},"obj":"NCBITaxon:10088"},{"id":"T15211","span":{"begin":29,"end":33},"obj":"PR:000033987"},{"id":"T15212","span":{"begin":34,"end":38},"obj":"SO:0000704"},{"id":"T15213","span":{"begin":53,"end":57},"obj":"PR:000017653"},{"id":"T15214","span":{"begin":58,"end":66},"obj":"SO:0000167"},{"id":"T15215","span":{"begin":112,"end":119},"obj":"NCBITaxon:33208"},{"id":"T15216","span":{"begin":125,"end":129},"obj":"PR:000033987"},{"id":"T15217","span":{"begin":130,"end":134},"obj":"SO:0000704"},{"id":"T15218","span":{"begin":175,"end":179},"obj":"PR:000017653"},{"id":"T15219","span":{"begin":211,"end":221},"obj":"GO:0010467"},{"id":"T15220","span":{"begin":242,"end":250},"obj":"SO:0001817"},{"id":"T15221","span":{"begin":289,"end":293},"obj":"PR:000017653"},{"id":"T15222","span":{"begin":307,"end":311},"obj":"PR:000017653"},{"id":"T15223","span":{"begin":312,"end":316},"obj":"PR:000033987"},{"id":"T15224","span":{"begin":342,"end":346},"obj":"SO:0000243"},{"id":"T15225","span":{"begin":352,"end":360},"obj":"SO:0005853"},{"id":"T15226","span":{"begin":382,"end":388},"obj":"SO:0001023"},{"id":"T15227","span":{"begin":392,"end":396},"obj":"PR:000017653"},{"id":"T15228","span":{"begin":397,"end":401},"obj":"SO:0000704"},{"id":"T15229","span":{"begin":407,"end":411},"obj":"PR:000017653"},{"id":"T15230","span":{"begin":412,"end":416},"obj":"PR:000033987"},{"id":"T15231","span":{"begin":489,"end":497},"obj":"CL:0002322"},{"id":"T15232","span":{"begin":598,"end":609},"obj":"UBERON:0000358"},{"id":"T15233","span":{"begin":611,"end":615},"obj":"PR:000017653"},{"id":"T15234","span":{"begin":616,"end":620},"obj":"PR:000033987"},{"id":"T15235","span":{"begin":626,"end":633},"obj":"NCBITaxon:33208"},{"id":"T15236","span":{"begin":688,"end":692},"obj":"PR:000033987"},{"id":"T15237","span":{"begin":693,"end":703},"obj":"GO:0010467"},{"id":"T15238","span":{"begin":723,"end":732},"obj":"UBERON:0000922"},{"id":"T15239","span":{"begin":733,"end":738},"obj":"UBERON:0002048"},{"id":"T15240","span":{"begin":798,"end":803},"obj":"CHEBI:75055"}],"text":"Transgenic mice carrying the lacZ gene driven by the Fog2 promoter have been developed by S. Tevosian. In these animals, the lacZ gene is incorporated (“knocked-in”) into the Fog2 locus to allow β-galactosidase expression as a fusion protein in frame with the first 235 amino acids of the FOG2 protein. The Fog2-lacZ module is followed by an ires-eGFP cassette. This creates a null allele of Fog2 gene. The Fog2-LacZ-eGFP construct was linearized with KspI and electroporated into the CJ7 ES cells. The correctly targeted clone was selected by the Southern blot analysis and injected into C57BL/6J blastocysts. Fog2-lacZ-eGFP animals were maintained on the mixed C57BL/6J/129 background. lacZ Expression in whole dissected embryonic lungs was analyzed by staining for β-galactosidase activity with X-gal after fixation for 30 min."}

{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T15270","span":{"begin":0,"end":10},"obj":"SO_EXT:transgenic_entity"},{"id":"T15271","span":{"begin":11,"end":15},"obj":"NCBITaxon:10088"},{"id":"T15272","span":{"begin":29,"end":33},"obj":"PR_EXT:000033987"},{"id":"T15273","span":{"begin":34,"end":38},"obj":"SO_EXT:0000704"},{"id":"T15274","span":{"begin":53,"end":57},"obj":"PR_EXT:000017653"},{"id":"T15275","span":{"begin":58,"end":66},"obj":"SO_EXT:0000167"},{"id":"T15276","span":{"begin":112,"end":119},"obj":"NCBITaxon:33208"},{"id":"T15277","span":{"begin":125,"end":129},"obj":"PR_EXT:000033987"},{"id":"T15278","span":{"begin":130,"end":134},"obj":"SO_EXT:0000704"},{"id":"T15279","span":{"begin":175,"end":179},"obj":"PR_EXT:000017653"},{"id":"T15280","span":{"begin":195,"end":210},"obj":"GO_EXT:0004565"},{"id":"T15281","span":{"begin":211,"end":221},"obj":"GO:0010467"},{"id":"T15282","span":{"begin":227,"end":233},"obj":"SO_EXT:sequence_fusion_entity_or_process"},{"id":"T15283","span":{"begin":234,"end":241},"obj":"CHEBI_PR_EXT:protein"},{"id":"T15284","span":{"begin":242,"end":250},"obj":"SO:0001817"},{"id":"T15285","span":{"begin":270,"end":281},"obj":"CHEBI_SO_EXT:amino_acid"},{"id":"T15286","span":{"begin":289,"end":293},"obj":"PR_EXT:000017653"},{"id":"T15287","span":{"begin":294,"end":301},"obj":"CHEBI_PR_EXT:protein"},{"id":"T15288","span":{"begin":307,"end":311},"obj":"PR_EXT:000017653"},{"id":"T15289","span":{"begin":312,"end":316},"obj":"PR_EXT:000033987"},{"id":"T15290","span":{"begin":342,"end":346},"obj":"SO_EXT:0000243"},{"id":"T15291","span":{"begin":352,"end":360},"obj":"SO_EXT:0005853"},{"id":"T15292","span":{"begin":377,"end":381},"obj":"SO_EXT:sequence_nullness"},{"id":"T15293","span":{"begin":382,"end":388},"obj":"SO_EXT:0001023"},{"id":"T15294","span":{"begin":392,"end":396},"obj":"PR_EXT:000017653"},{"id":"T15295","span":{"begin":397,"end":401},"obj":"SO_EXT:0000704"},{"id":"T15296","span":{"begin":407,"end":411},"obj":"PR_EXT:000017653"},{"id":"T15297","span":{"begin":412,"end":416},"obj":"PR_EXT:000033987"},{"id":"T15298","span":{"begin":422,"end":431},"obj":"SO_EXT:engineered_biological_sequence"},{"id":"T15299","span":{"begin":436,"end":446},"obj":"SO_EXT:sequence_linearization"},{"id":"T15300","span":{"begin":489,"end":497},"obj":"CL:0002322"},{"id":"T15301","span":{"begin":492,"end":497},"obj":"CL_GO_EXT:cell"},{"id":"T15302","span":{"begin":522,"end":527},"obj":"SO_EXT:sequence_cloned_entity"},{"id":"T15303","span":{"begin":598,"end":609},"obj":"UBERON:0000358"},{"id":"T15304","span":{"begin":611,"end":615},"obj":"PR_EXT:000017653"},{"id":"T15305","span":{"begin":616,"end":620},"obj":"PR_EXT:000033987"},{"id":"T15306","span":{"begin":626,"end":633},"obj":"NCBITaxon:33208"},{"id":"T15307","span":{"begin":688,"end":692},"obj":"PR_EXT:000033987"},{"id":"T15308","span":{"begin":693,"end":703},"obj":"GO:0010467"},{"id":"T15309","span":{"begin":723,"end":732},"obj":"UBERON:0000922"},{"id":"T15310","span":{"begin":733,"end":738},"obj":"UBERON:0002048"},{"id":"T15311","span":{"begin":768,"end":783},"obj":"GO_EXT:0015925"},{"id":"T15312","span":{"begin":798,"end":803},"obj":"CHEBI:75055"}],"text":"Transgenic mice carrying the lacZ gene driven by the Fog2 promoter have been developed by S. Tevosian. In these animals, the lacZ gene is incorporated (“knocked-in”) into the Fog2 locus to allow β-galactosidase expression as a fusion protein in frame with the first 235 amino acids of the FOG2 protein. The Fog2-lacZ module is followed by an ires-eGFP cassette. This creates a null allele of Fog2 gene. The Fog2-LacZ-eGFP construct was linearized with KspI and electroporated into the CJ7 ES cells. The correctly targeted clone was selected by the Southern blot analysis and injected into C57BL/6J blastocysts. Fog2-lacZ-eGFP animals were maintained on the mixed C57BL/6J/129 background. lacZ Expression in whole dissected embryonic lungs was analyzed by staining for β-galactosidase activity with X-gal after fixation for 30 min."}

{"project":"craft-sa-dev","denotations":[{"id":"T15547","span":{"begin":0,"end":10},"obj":"JJ"},{"id":"T15548","span":{"begin":11,"end":15},"obj":"NNS"},{"id":"T15549","span":{"begin":77,"end":86},"obj":"VBN"},{"id":"T15550","span":{"begin":16,"end":24},"obj":"VBG"},{"id":"T15551","span":{"begin":25,"end":28},"obj":"DT"},{"id":"T15552","span":{"begin":34,"end":38},"obj":"NN"},{"id":"T15553","span":{"begin":29,"end":33},"obj":"NN"},{"id":"T15554","span":{"begin":39,"end":45},"obj":"VBN"},{"id":"T15555","span":{"begin":46,"end":48},"obj":"IN"},{"id":"T15556","span":{"begin":49,"end":52},"obj":"DT"},{"id":"T15557","span":{"begin":58,"end":66},"obj":"NN"},{"id":"T15558","span":{"begin":53,"end":57},"obj":"NN"},{"id":"T15559","span":{"begin":67,"end":71},"obj":"VBP"},{"id":"T15560","span":{"begin":72,"end":76},"obj":"VBN"},{"id":"T15561","span":{"begin":87,"end":89},"obj":"IN"},{"id":"T15562","span":{"begin":90,"end":92},"obj":"NNP"},{"id":"T15563","span":{"begin":93,"end":101},"obj":"NNP"},{"id":"T15564","span":{"begin":101,"end":102},"obj":"."},{"id":"T15565","span":{"begin":102,"end":302},"obj":"sentence"},{"id":"T15566","span":{"begin":103,"end":105},"obj":"IN"},{"id":"T15567","span":{"begin":138,"end":150},"obj":"VBN"},{"id":"T15568","span":{"begin":106,"end":111},"obj":"DT"},{"id":"T15569","span":{"begin":112,"end":119},"obj":"NNS"},{"id":"T15570","span":{"begin":119,"end":121},"obj":","},{"id":"T15571","span":{"begin":121,"end":124},"obj":"DT"},{"id":"T15572","span":{"begin":130,"end":134},"obj":"NN"},{"id":"T15573","span":{"begin":125,"end":129},"obj":"NN"},{"id":"T15574","span":{"begin":135,"end":137},"obj":"VBZ"},{"id":"T15575","span":{"begin":151,"end":152},"obj":"-LRB-"},{"id":"T15576","span":{"begin":153,"end":160},"obj":"VBN"},{"id":"T15577","span":{"begin":152,"end":153},"obj":"``"},{"id":"T15578","span":{"begin":160,"end":161},"obj":"HYPH"},{"id":"T15579","span":{"begin":161,"end":163},"obj":"RP"},{"id":"T15580","span":{"begin":163,"end":164},"obj":"''"},{"id":"T15581","span":{"begin":164,"end":165},"obj":"-RRB-"},{"id":"T15582","span":{"begin":166,"end":170},"obj":"IN"},{"id":"T15583","span":{"begin":171,"end":174},"obj":"DT"},{"id":"T15584","span":{"begin":180,"end":185},"obj":"NN"},{"id":"T15585","span":{"begin":175,"end":179},"obj":"NN"},{"id":"T15586","span":{"begin":186,"end":188},"obj":"TO"},{"id":"T15587","span":{"begin":189,"end":194},"obj":"VB"},{"id":"T15588","span":{"begin":195,"end":196},"obj":"NN"},{"id":"T15589","span":{"begin":197,"end":210},"obj":"NN"},{"id":"T15590","span":{"begin":196,"end":197},"obj":"HYPH"},{"id":"T15591","span":{"begin":211,"end":221},"obj":"NN"},{"id":"T15592","span":{"begin":222,"end":224},"obj":"IN"},{"id":"T15593","span":{"begin":225,"end":226},"obj":"DT"},{"id":"T15594","span":{"begin":234,"end":241},"obj":"NN"},{"id":"T15595","span":{"begin":227,"end":233},"obj":"NN"},{"id":"T15596","span":{"begin":242,"end":244},"obj":"IN"},{"id":"T15597","span":{"begin":245,"end":250},"obj":"NN"},{"id":"T15598","span":{"begin":251,"end":255},"obj":"IN"},{"id":"T15599","span":{"begin":256,"end":259},"obj":"DT"},{"id":"T15600","span":{"begin":276,"end":281},"obj":"NNS"},{"id":"T15601","span":{"begin":260,"end":265},"obj":"JJ"},{"id":"T15602","span":{"begin":266,"end":269},"obj":"CD"},{"id":"T15603","span":{"begin":270,"end":275},"obj":"NN"},{"id":"T15604","span":{"begin":282,"end":284},"obj":"IN"},{"id":"T15605","span":{"begin":285,"end":288},"obj":"DT"},{"id":"T15606","span":{"begin":294,"end":301},"obj":"NN"},{"id":"T15607","span":{"begin":289,"end":293},"obj":"NN"},{"id":"T15608","span":{"begin":301,"end":302},"obj":"."},{"id":"T15609","span":{"begin":302,"end":361},"obj":"sentence"},{"id":"T15610","span":{"begin":303,"end":306},"obj":"DT"},{"id":"T15611","span":{"begin":317,"end":323},"obj":"NN"},{"id":"T15612","span":{"begin":307,"end":311},"obj":"NN"},{"id":"T15613","span":{"begin":312,"end":316},"obj":"NN"},{"id":"T15614","span":{"begin":311,"end":312},"obj":"HYPH"},{"id":"T15615","span":{"begin":327,"end":335},"obj":"VBN"},{"id":"T15616","span":{"begin":324,"end":326},"obj":"VBZ"},{"id":"T15617","span":{"begin":336,"end":338},"obj":"IN"},{"id":"T15618","span":{"begin":339,"end":341},"obj":"DT"},{"id":"T15619","span":{"begin":352,"end":360},"obj":"NN"},{"id":"T15620","span":{"begin":342,"end":346},"obj":"NN"},{"id":"T15621","span":{"begin":347,"end":351},"obj":"NN"},{"id":"T15622","span":{"begin":346,"end":347},"obj":"HYPH"},{"id":"T15623","span":{"begin":360,"end":361},"obj":"."},{"id":"T15624","span":{"begin":361,"end":402},"obj":"sentence"},{"id":"T15625","span":{"begin":362,"end":366},"obj":"DT"},{"id":"T15626","span":{"begin":367,"end":374},"obj":"VBZ"},{"id":"T15627","span":{"begin":375,"end":376},"obj":"DT"},{"id":"T15628","span":{"begin":382,"end":388},"obj":"NN"},{"id":"T15629","span":{"begin":377,"end":381},"obj":"JJ"},{"id":"T15630","span":{"begin":389,"end":391},"obj":"IN"},{"id":"T15631","span":{"begin":392,"end":396},"obj":"NN"},{"id":"T15632","span":{"begin":397,"end":401},"obj":"NN"},{"id":"T15633","span":{"begin":401,"end":402},"obj":"."},{"id":"T15634","span":{"begin":402,"end":498},"obj":"sentence"},{"id":"T15635","span":{"begin":403,"end":406},"obj":"DT"},{"id":"T15636","span":{"begin":422,"end":431},"obj":"NN"},{"id":"T15637","span":{"begin":407,"end":411},"obj":"NN"},{"id":"T15638","span":{"begin":417,"end":421},"obj":"NN"},{"id":"T15639","span":{"begin":411,"end":412},"obj":"HYPH"},{"id":"T15640","span":{"begin":412,"end":416},"obj":"NN"},{"id":"T15641","span":{"begin":416,"end":417},"obj":"HYPH"},{"id":"T15642","span":{"begin":436,"end":446},"obj":"VBN"},{"id":"T15643","span":{"begin":432,"end":435},"obj":"VBD"},{"id":"T15644","span":{"begin":447,"end":451},"obj":"IN"},{"id":"T15645","span":{"begin":452,"end":456},"obj":"NN"},{"id":"T15646","span":{"begin":457,"end":460},"obj":"CC"},{"id":"T15647","span":{"begin":461,"end":475},"obj":"VBN"},{"id":"T15648","span":{"begin":476,"end":480},"obj":"IN"},{"id":"T15649","span":{"begin":481,"end":484},"obj":"DT"},{"id":"T15650","span":{"begin":492,"end":497},"obj":"NNS"},{"id":"T15651","span":{"begin":485,"end":488},"obj":"NN"},{"id":"T15652","span":{"begin":489,"end":491},"obj":"NN"},{"id":"T15653","span":{"begin":497,"end":498},"obj":"."},{"id":"T15654","span":{"begin":498,"end":610},"obj":"sentence"},{"id":"T15655","span":{"begin":499,"end":502},"obj":"DT"},{"id":"T15656","span":{"begin":522,"end":527},"obj":"NN"},{"id":"T15657","span":{"begin":503,"end":512},"obj":"RB"},{"id":"T15658","span":{"begin":513,"end":521},"obj":"VBN"},{"id":"T15659","span":{"begin":532,"end":540},"obj":"VBN"},{"id":"T15660","span":{"begin":528,"end":531},"obj":"VBD"},{"id":"T15661","span":{"begin":541,"end":543},"obj":"IN"},{"id":"T15662","span":{"begin":544,"end":547},"obj":"DT"},{"id":"T15663","span":{"begin":562,"end":570},"obj":"NN"},{"id":"T15664","span":{"begin":548,"end":556},"obj":"NNP"},{"id":"T15665","span":{"begin":557,"end":561},"obj":"NN"},{"id":"T15666","span":{"begin":571,"end":574},"obj":"CC"},{"id":"T15667","span":{"begin":575,"end":583},"obj":"VBN"},{"id":"T15668","span":{"begin":584,"end":588},"obj":"IN"},{"id":"T15669","span":{"begin":589,"end":594},"obj":"NN"},{"id":"T15670","span":{"begin":595,"end":597},"obj":"NN"},{"id":"T15671","span":{"begin":594,"end":595},"obj":"HYPH"},{"id":"T15672","span":{"begin":598,"end":609},"obj":"NNS"},{"id":"T15673","span":{"begin":609,"end":610},"obj":"."},{"id":"T15674","span":{"begin":610,"end":687},"obj":"sentence"},{"id":"T15675","span":{"begin":611,"end":615},"obj":"NN"},{"id":"T15676","span":{"begin":621,"end":625},"obj":"NN"},{"id":"T15677","span":{"begin":615,"end":616},"obj":"HYPH"},{"id":"T15678","span":{"begin":616,"end":620},"obj":"NN"},{"id":"T15679","span":{"begin":620,"end":621},"obj":"HYPH"},{"id":"T15680","span":{"begin":626,"end":633},"obj":"NNS"},{"id":"T15681","span":{"begin":639,"end":649},"obj":"VBN"},{"id":"T15682","span":{"begin":634,"end":638},"obj":"VBD"},{"id":"T15683","span":{"begin":650,"end":652},"obj":"IN"},{"id":"T15684","span":{"begin":653,"end":656},"obj":"DT"},{"id":"T15685","span":{"begin":676,"end":686},"obj":"NN"},{"id":"T15686","span":{"begin":657,"end":662},"obj":"JJ"},{"id":"T15687","span":{"begin":663,"end":668},"obj":"NN"},{"id":"T15688","span":{"begin":669,"end":671},"obj":"NN"},{"id":"T15689","span":{"begin":668,"end":669},"obj":"HYPH"},{"id":"T15690","span":{"begin":671,"end":672},"obj":"HYPH"},{"id":"T15691","span":{"begin":672,"end":675},"obj":"CD"},{"id":"T15692","span":{"begin":686,"end":687},"obj":"."},{"id":"T15693","span":{"begin":687,"end":830},"obj":"sentence"},{"id":"T15694","span":{"begin":688,"end":692},"obj":"NN"},{"id":"T15695","span":{"begin":693,"end":703},"obj":"NN"},{"id":"T15696","span":{"begin":743,"end":751},"obj":"VBN"},{"id":"T15697","span":{"begin":704,"end":706},"obj":"IN"},{"id":"T15698","span":{"begin":707,"end":712},"obj":"JJ"},{"id":"T15699","span":{"begin":733,"end":738},"obj":"NNS"},{"id":"T15700","span":{"begin":713,"end":722},"obj":"VBN"},{"id":"T15701","span":{"begin":723,"end":732},"obj":"JJ"},{"id":"T15702","span":{"begin":739,"end":742},"obj":"VBD"},{"id":"T15703","span":{"begin":752,"end":754},"obj":"IN"},{"id":"T15704","span":{"begin":755,"end":763},"obj":"VBG"},{"id":"T15705","span":{"begin":764,"end":767},"obj":"IN"},{"id":"T15706","span":{"begin":768,"end":769},"obj":"NN"},{"id":"T15707","span":{"begin":770,"end":783},"obj":"NN"},{"id":"T15708","span":{"begin":769,"end":770},"obj":"HYPH"},{"id":"T15709","span":{"begin":784,"end":792},"obj":"NN"},{"id":"T15710","span":{"begin":793,"end":797},"obj":"IN"},{"id":"T15711","span":{"begin":798,"end":799},"obj":"NN"},{"id":"T15712","span":{"begin":800,"end":803},"obj":"NN"},{"id":"T15713","span":{"begin":799,"end":800},"obj":"HYPH"},{"id":"T15714","span":{"begin":804,"end":809},"obj":"IN"},{"id":"T15715","span":{"begin":810,"end":818},"obj":"NN"},{"id":"T15716","span":{"begin":819,"end":822},"obj":"IN"},{"id":"T15717","span":{"begin":823,"end":825},"obj":"CD"},{"id":"T15718","span":{"begin":826,"end":829},"obj":"NN"},{"id":"T15719","span":{"begin":829,"end":830},"obj":"."}],"relations":[{"id":"R4421","pred":"amod","subj":"T15547","obj":"T15548"},{"id":"R4422","pred":"nsubjpass","subj":"T15548","obj":"T15549"},{"id":"R4423","pred":"acl","subj":"T15550","obj":"T15548"},{"id":"R4424","pred":"det","subj":"T15551","obj":"T15552"},{"id":"R4425","pred":"dobj","subj":"T15552","obj":"T15550"},{"id":"R4426","pred":"compound","subj":"T15553","obj":"T15552"},{"id":"R4427","pred":"acl","subj":"T15554","obj":"T15552"},{"id":"R4428","pred":"agent","subj":"T15555","obj":"T15554"},{"id":"R4429","pred":"det","subj":"T15556","obj":"T15557"},{"id":"R4430","pred":"pobj","subj":"T15557","obj":"T15555"},{"id":"R4431","pred":"compound","subj":"T15558","obj":"T15557"},{"id":"R4432","pred":"aux","subj":"T15559","obj":"T15549"},{"id":"R4433","pred":"auxpass","subj":"T15560","obj":"T15549"},{"id":"R4434","pred":"agent","subj":"T15561","obj":"T15549"},{"id":"R4435","pred":"compound","subj":"T15562","obj":"T15563"},{"id":"R4436","pred":"pobj","subj":"T15563","obj":"T15561"},{"id":"R4437","pred":"punct","subj":"T15564","obj":"T15549"},{"id":"R4438","pred":"prep","subj":"T15566","obj":"T15567"},{"id":"R4439","pred":"det","subj":"T15568","obj":"T15569"},{"id":"R4440","pred":"pobj","subj":"T15569","obj":"T15566"},{"id":"R4441","pred":"punct","subj":"T15570","obj":"T15567"},{"id":"R4442","pred":"det","subj":"T15571","obj":"T15572"},{"id":"R4443","pred":"nsubjpass","subj":"T15572","obj":"T15567"},{"id":"R4444","pred":"compound","subj":"T15573","obj":"T15572"},{"id":"R4445","pred":"auxpass","subj":"T15574","obj":"T15567"},{"id":"R4446","pred":"punct","subj":"T15575","obj":"T15576"},{"id":"R4447","pred":"parataxis","subj":"T15576","obj":"T15567"},{"id":"R4448","pred":"punct","subj":"T15577","obj":"T15576"},{"id":"R4449","pred":"punct","subj":"T15578","obj":"T15576"},{"id":"R4450","pred":"prt","subj":"T15579","obj":"T15576"},{"id":"R4451","pred":"punct","subj":"T15580","obj":"T15576"},{"id":"R4452","pred":"punct","subj":"T15581","obj":"T15576"},{"id":"R4453","pred":"prep","subj":"T15582","obj":"T15567"},{"id":"R4454","pred":"det","subj":"T15583","obj":"T15584"},{"id":"R4455","pred":"pobj","subj":"T15584","obj":"T15582"},{"id":"R4456","pred":"compound","subj":"T15585","obj":"T15584"},{"id":"R4457","pred":"aux","subj":"T15586","obj":"T15587"},{"id":"R4458","pred":"advcl","subj":"T15587","obj":"T15567"},{"id":"R4459","pred":"compound","subj":"T15588","obj":"T15589"},{"id":"R4460","pred":"compound","subj":"T15589","obj":"T15591"},{"id":"R4461","pred":"punct","subj":"T15590","obj":"T15589"},{"id":"R4462","pred":"dobj","subj":"T15591","obj":"T15587"},{"id":"R4463","pred":"prep","subj":"T15592","obj":"T15587"},{"id":"R4464","pred":"det","subj":"T15593","obj":"T15594"},{"id":"R4465","pred":"pobj","subj":"T15594","obj":"T15592"},{"id":"R4466","pred":"compound","subj":"T15595","obj":"T15594"},{"id":"R4467","pred":"prep","subj":"T15596","obj":"T15594"},{"id":"R4468","pred":"pobj","subj":"T15597","obj":"T15596"},{"id":"R4469","pred":"prep","subj":"T15598","obj":"T15597"},{"id":"R4470","pred":"det","subj":"T15599","obj":"T15600"},{"id":"R4471","pred":"pobj","subj":"T15600","obj":"T15598"},{"id":"R4472","pred":"amod","subj":"T15601","obj":"T15600"},{"id":"R4473","pred":"nummod","subj":"T15602","obj":"T15600"},{"id":"R4474","pred":"compound","subj":"T15603","obj":"T15600"},{"id":"R4475","pred":"prep","subj":"T15604","obj":"T15600"},{"id":"R4476","pred":"det","subj":"T15605","obj":"T15606"},{"id":"R4477","pred":"pobj","subj":"T15606","obj":"T15604"},{"id":"R4478","pred":"compound","subj":"T15607","obj":"T15606"},{"id":"R4479","pred":"punct","subj":"T15608","obj":"T15567"},{"id":"R4480","pred":"det","subj":"T15610","obj":"T15611"},{"id":"R4481","pred":"nsubjpass","subj":"T15611","obj":"T15615"},{"id":"R4482","pred":"compound","subj":"T15612","obj":"T15613"},{"id":"R4483","pred":"compound","subj":"T15613","obj":"T15611"},{"id":"R4484","pred":"punct","subj":"T15614","obj":"T15613"},{"id":"R4485","pred":"auxpass","subj":"T15616","obj":"T15615"},{"id":"R4486","pred":"agent","subj":"T15617","obj":"T15615"},{"id":"R4487","pred":"det","subj":"T15618","obj":"T15619"},{"id":"R4488","pred":"pobj","subj":"T15619","obj":"T15617"},{"id":"R4489","pred":"compound","subj":"T15620","obj":"T15621"},{"id":"R4490","pred":"compound","subj":"T15621","obj":"T15619"},{"id":"R4491","pred":"punct","subj":"T15622","obj":"T15621"},{"id":"R4492","pred":"punct","subj":"T15623","obj":"T15615"},{"id":"R4493","pred":"nsubj","subj":"T15625","obj":"T15626"},{"id":"R4494","pred":"det","subj":"T15627","obj":"T15628"},{"id":"R4495","pred":"dobj","subj":"T15628","obj":"T15626"},{"id":"R4496","pred":"amod","subj":"T15629","obj":"T15628"},{"id":"R4497","pred":"prep","subj":"T15630","obj":"T15628"},{"id":"R4498","pred":"compound","subj":"T15631","obj":"T15632"},{"id":"R4499","pred":"pobj","subj":"T15632","obj":"T15630"},{"id":"R4500","pred":"punct","subj":"T15633","obj":"T15626"},{"id":"R4501","pred":"det","subj":"T15635","obj":"T15636"},{"id":"R4502","pred":"nsubjpass","subj":"T15636","obj":"T15642"},{"id":"R4503","pred":"compound","subj":"T15637","obj":"T15638"},{"id":"R4504","pred":"compound","subj":"T15638","obj":"T15636"},{"id":"R4505","pred":"punct","subj":"T15639","obj":"T15638"},{"id":"R4506","pred":"compound","subj":"T15640","obj":"T15638"},{"id":"R4507","pred":"punct","subj":"T15641","obj":"T15638"},{"id":"R4508","pred":"auxpass","subj":"T15643","obj":"T15642"},{"id":"R4509","pred":"prep","subj":"T15644","obj":"T15642"},{"id":"R4510","pred":"pobj","subj":"T15645","obj":"T15644"},{"id":"R4511","pred":"cc","subj":"T15646","obj":"T15642"},{"id":"R4512","pred":"conj","subj":"T15647","obj":"T15642"},{"id":"R4513","pred":"prep","subj":"T15648","obj":"T15647"},{"id":"R4514","pred":"det","subj":"T15649","obj":"T15650"},{"id":"R4515","pred":"pobj","subj":"T15650","obj":"T15648"},{"id":"R4516","pred":"compound","subj":"T15651","obj":"T15650"},{"id":"R4517","pred":"compound","subj":"T15652","obj":"T15650"},{"id":"R4518","pred":"punct","subj":"T15653","obj":"T15642"},{"id":"R4519","pred":"det","subj":"T15655","obj":"T15656"},{"id":"R4520","pred":"nsubjpass","subj":"T15656","obj":"T15659"},{"id":"R4521","pred":"advmod","subj":"T15657","obj":"T15658"},{"id":"R4522","pred":"amod","subj":"T15658","obj":"T15656"},{"id":"R4523","pred":"auxpass","subj":"T15660","obj":"T15659"},{"id":"R4524","pred":"prep","subj":"T15661","obj":"T15659"},{"id":"R4525","pred":"det","subj":"T15662","obj":"T15663"},{"id":"R4526","pred":"pobj","subj":"T15663","obj":"T15661"},{"id":"R4527","pred":"compound","subj":"T15664","obj":"T15665"},{"id":"R4528","pred":"compound","subj":"T15665","obj":"T15663"},{"id":"R4529","pred":"cc","subj":"T15666","obj":"T15659"},{"id":"R4530","pred":"conj","subj":"T15667","obj":"T15659"},{"id":"R4531","pred":"prep","subj":"T15668","obj":"T15667"},{"id":"R4532","pred":"compound","subj":"T15669","obj":"T15670"},{"id":"R4533","pred":"compound","subj":"T15670","obj":"T15672"},{"id":"R4534","pred":"punct","subj":"T15671","obj":"T15670"},{"id":"R4535","pred":"pobj","subj":"T15672","obj":"T15668"},{"id":"R4536","pred":"punct","subj":"T15673","obj":"T15659"},{"id":"R4537","pred":"compound","subj":"T15675","obj":"T15676"},{"id":"R4538","pred":"compound","subj":"T15676","obj":"T15680"},{"id":"R4539","pred":"punct","subj":"T15677","obj":"T15676"},{"id":"R4540","pred":"compound","subj":"T15678","obj":"T15676"},{"id":"R4541","pred":"punct","subj":"T15679","obj":"T15676"},{"id":"R4542","pred":"nsubjpass","subj":"T15680","obj":"T15681"},{"id":"R4543","pred":"auxpass","subj":"T15682","obj":"T15681"},{"id":"R4544","pred":"prep","subj":"T15683","obj":"T15681"},{"id":"R4545","pred":"det","subj":"T15684","obj":"T15685"},{"id":"R4546","pred":"pobj","subj":"T15685","obj":"T15683"},{"id":"R4547","pred":"amod","subj":"T15686","obj":"T15685"},{"id":"R4548","pred":"nmod","subj":"T15687","obj":"T15688"},{"id":"R4549","pred":"nmod","subj":"T15688","obj":"T15685"},{"id":"R4550","pred":"punct","subj":"T15689","obj":"T15688"},{"id":"R4551","pred":"punct","subj":"T15690","obj":"T15688"},{"id":"R4552","pred":"nummod","subj":"T15691","obj":"T15688"},{"id":"R4553","pred":"punct","subj":"T15692","obj":"T15681"},{"id":"R4554","pred":"compound","subj":"T15694","obj":"T15695"},{"id":"R4555","pred":"nsubjpass","subj":"T15695","obj":"T15696"},{"id":"R4556","pred":"prep","subj":"T15697","obj":"T15695"},{"id":"R4557","pred":"amod","subj":"T15698","obj":"T15699"},{"id":"R4558","pred":"pobj","subj":"T15699","obj":"T15697"},{"id":"R4559","pred":"amod","subj":"T15700","obj":"T15699"},{"id":"R4560","pred":"amod","subj":"T15701","obj":"T15699"},{"id":"R4561","pred":"auxpass","subj":"T15702","obj":"T15696"},{"id":"R4562","pred":"prep","subj":"T15703","obj":"T15696"},{"id":"R4563","pred":"pcomp","subj":"T15704","obj":"T15703"},{"id":"R4564","pred":"prep","subj":"T15705","obj":"T15704"},{"id":"R4565","pred":"compound","subj":"T15706","obj":"T15707"},{"id":"R4566","pred":"compound","subj":"T15707","obj":"T15709"},{"id":"R4567","pred":"punct","subj":"T15708","obj":"T15707"},{"id":"R4568","pred":"pobj","subj":"T15709","obj":"T15705"},{"id":"R4569","pred":"prep","subj":"T15710","obj":"T15704"},{"id":"R4570","pred":"compound","subj":"T15711","obj":"T15712"},{"id":"R4571","pred":"pobj","subj":"T15712","obj":"T15710"},{"id":"R4572","pred":"punct","subj":"T15713","obj":"T15712"},{"id":"R4573","pred":"prep","subj":"T15714","obj":"T15704"},{"id":"R4574","pred":"pobj","subj":"T15715","obj":"T15714"},{"id":"R4575","pred":"prep","subj":"T15716","obj":"T15715"},{"id":"R4576","pred":"nummod","subj":"T15717","obj":"T15718"},{"id":"R4577","pred":"pobj","subj":"T15718","obj":"T15716"},{"id":"R4578","pred":"punct","subj":"T15719","obj":"T15696"}],"text":"Transgenic mice carrying the lacZ gene driven by the Fog2 promoter have been developed by S. Tevosian. In these animals, the lacZ gene is incorporated (“knocked-in”) into the Fog2 locus to allow β-galactosidase expression as a fusion protein in frame with the first 235 amino acids of the FOG2 protein. The Fog2-lacZ module is followed by an ires-eGFP cassette. This creates a null allele of Fog2 gene. The Fog2-LacZ-eGFP construct was linearized with KspI and electroporated into the CJ7 ES cells. The correctly targeted clone was selected by the Southern blot analysis and injected into C57BL/6J blastocysts. Fog2-lacZ-eGFP animals were maintained on the mixed C57BL/6J/129 background. lacZ Expression in whole dissected embryonic lungs was analyzed by staining for β-galactosidase activity with X-gal after fixation for 30 min."}