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All four ES cell lines transmitted through the germline, leading to the establishment of, CK4/ECFP and CK6/ECFP, two strains of mice having cyan fluorescent protein expression and, YC5/EYFP and 2A4/EYFP, two strains having widespread yellow fluorescent protein expression. In these mice the respective FP expression is constituitive, starting at preimplantation stages and continuing through postimplantation embryogenesis to adulthood (Fig. 2). One line expressing each color was chosen for further analysis and breeding to homozygosity. The availability of homozygous animals demonstrated that both reporter gene expression and the site of transgene integration were developmentally neutral."}

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{"project":"craft-ca-core-dev","denotations":[{"id":"T1166","span":{"begin":101,"end":108},"obj":"CL:0002322"},{"id":"T1167","span":{"begin":113,"end":119},"obj":"UBERON:0000085"},{"id":"T1168","span":{"begin":126,"end":132},"obj":"UBERON:0000922"},{"id":"T1169","span":{"begin":160,"end":167},"obj":"CL:0002322"},{"id":"T1170","span":{"begin":279,"end":283},"obj":"NCBITaxon:10088"},{"id":"T1171","span":{"begin":316,"end":326},"obj":"GO:0010467"},{"id":"T1172","span":{"begin":412,"end":422},"obj":"GO:0010467"},{"id":"T1173","span":{"begin":433,"end":437},"obj":"NCBITaxon:10088"},{"id":"T1174","span":{"begin":456,"end":466},"obj":"GO:0010467"},{"id":"T1175","span":{"begin":500,"end":512},"obj":"GO:0007566"},{"id":"T1176","span":{"begin":547,"end":559},"obj":"GO:0007566"},{"id":"T1177","span":{"begin":560,"end":573},"obj":"GO:0009790"},{"id":"T1178","span":{"begin":577,"end":586},"obj":"UBERON:0000113"},{"id":"T1179","span":{"begin":606,"end":616},"obj":"GO:0010467"},{"id":"T1180","span":{"begin":721,"end":728},"obj":"NCBITaxon:33208"},{"id":"T1181","span":{"begin":761,"end":765},"obj":"SO:0000704"},{"id":"T1182","span":{"begin":761,"end":776},"obj":"GO:0010467"},{"id":"T1183","span":{"begin":785,"end":792},"obj":"_FRAGMENT"},{"id":"T1184","span":{"begin":803,"end":814},"obj":"SO:0000366"},{"id":"T1185","span":{"begin":793,"end":802},"obj":"SO:0000902"}],"relations":[{"id":"R702","pred":"_lexicallyChainedTo","subj":"T1184","obj":"T1183"}],"text":"Two ECFP+ and two EYFP+ clones exhibiting robust fluorescence were used to generate chimeras through ES cell \u003c-\u003e morula stage embryo aggregation [13]. All four ES cell lines transmitted through the germline, leading to the establishment of, CK4/ECFP and CK6/ECFP, two strains of mice having cyan fluorescent protein expression and, YC5/EYFP and 2A4/EYFP, two strains having widespread yellow fluorescent protein expression. In these mice the respective FP expression is constituitive, starting at preimplantation stages and continuing through postimplantation embryogenesis to adulthood (Fig. 2). One line expressing each color was chosen for further analysis and breeding to homozygosity. The availability of homozygous animals demonstrated that both reporter gene expression and the site of transgene integration were developmentally neutral."}

{"project":"2_test","denotations":[{"id":"12079497-8361547-8577608","span":{"begin":146,"end":148},"obj":"8361547"}],"text":"Two ECFP+ and two EYFP+ clones exhibiting robust fluorescence were used to generate chimeras through ES cell \u003c-\u003e morula stage embryo aggregation [13]. All four ES cell lines transmitted through the germline, leading to the establishment of, CK4/ECFP and CK6/ECFP, two strains of mice having cyan fluorescent protein expression and, YC5/EYFP and 2A4/EYFP, two strains having widespread yellow fluorescent protein expression. In these mice the respective FP expression is constituitive, starting at preimplantation stages and continuing through postimplantation embryogenesis to adulthood (Fig. 2). One line expressing each color was chosen for further analysis and breeding to homozygosity. The availability of homozygous animals demonstrated that both reporter gene expression and the site of transgene integration were developmentally neutral."}