Id |
Subject |
Object |
Predicate |
Lexical cue |
T2003 |
160-162 |
NN |
denotes |
ES |
T2004 |
163-167 |
NN |
denotes |
cell |
T2005 |
174-185 |
VBD |
denotes |
transmitted |
T2006 |
186-193 |
IN |
denotes |
through |
T2007 |
194-197 |
DT |
denotes |
the |
T2008 |
198-206 |
NN |
denotes |
germline |
T2009 |
206-208 |
, |
denotes |
, |
T2010 |
208-215 |
VBG |
denotes |
leading |
T2011 |
216-218 |
IN |
denotes |
to |
T2012 |
219-222 |
DT |
denotes |
the |
T2013 |
223-236 |
NN |
denotes |
establishment |
T2014 |
237-239 |
IN |
denotes |
of |
T2015 |
239-241 |
, |
denotes |
, |
T2016 |
241-244 |
NN |
denotes |
CK4 |
T2017 |
245-249 |
NN |
denotes |
ECFP |
T2018 |
244-245 |
HYPH |
denotes |
/ |
T2019 |
250-253 |
CC |
denotes |
and |
T2020 |
254-257 |
NN |
denotes |
CK6 |
T2021 |
258-262 |
NN |
denotes |
ECFP |
T2022 |
257-258 |
HYPH |
denotes |
/ |
T2023 |
262-264 |
, |
denotes |
, |
T2024 |
264-267 |
CD |
denotes |
two |
T2025 |
268-275 |
NNS |
denotes |
strains |
T2026 |
276-278 |
IN |
denotes |
of |
T2027 |
279-283 |
NNS |
denotes |
mice |
T2028 |
284-290 |
VBG |
denotes |
having |
T2029 |
291-295 |
NN |
denotes |
cyan |
T2030 |
308-315 |
NN |
denotes |
protein |
T2031 |
296-307 |
JJ |
denotes |
fluorescent |
T2032 |
316-326 |
NN |
denotes |
expression |
T2033 |
327-330 |
CC |
denotes |
and |
T2034 |
330-332 |
, |
denotes |
, |
T2035 |
332-335 |
NN |
denotes |
YC5 |
T2036 |
336-340 |
NN |
denotes |
EYFP |
T2037 |
335-336 |
HYPH |
denotes |
/ |
T2038 |
341-344 |
CC |
denotes |
and |
T2039 |
345-348 |
NN |
denotes |
2A4 |
T2040 |
349-353 |
NN |
denotes |
EYFP |
T2069 |
535-542 |
IN |
denotes |
through |
T2070 |
543-559 |
NN |
denotes |
postimplantation |
T2071 |
560-573 |
NN |
denotes |
embryogenesis |
T2072 |
574-576 |
IN |
denotes |
to |
T2073 |
577-586 |
NN |
denotes |
adulthood |
T2074 |
587-588 |
-LRB- |
denotes |
( |
T2075 |
588-592 |
NN |
denotes |
Fig. |
T2076 |
593-594 |
CD |
denotes |
2 |
T2077 |
594-595 |
-RRB- |
denotes |
) |
T2078 |
595-596 |
. |
denotes |
. |
T2079 |
596-689 |
sentence |
denotes |
One line expressing each color was chosen for further analysis and breeding to homozygosity. |
T2080 |
597-600 |
CD |
denotes |
One |
T2081 |
601-605 |
NN |
denotes |
line |
T2082 |
632-638 |
VBN |
denotes |
chosen |
T2083 |
606-616 |
VBG |
denotes |
expressing |
T2084 |
617-621 |
DT |
denotes |
each |
T2085 |
622-627 |
NN |
denotes |
color |
T2086 |
628-631 |
VBD |
denotes |
was |
T2087 |
639-642 |
IN |
denotes |
for |
T2088 |
643-650 |
JJ |
denotes |
further |
T2089 |
651-659 |
NN |
denotes |
analysis |
T2090 |
660-663 |
CC |
denotes |
and |
T2091 |
664-672 |
NN |
denotes |
breeding |
T2092 |
673-675 |
IN |
denotes |
to |
T2093 |
676-688 |
NN |
denotes |
homozygosity |
T2094 |
688-689 |
. |
denotes |
. |
T2095 |
689-844 |
sentence |
denotes |
The availability of homozygous animals demonstrated that both reporter gene expression and the site of transgene integration were developmentally neutral. |
T2096 |
690-693 |
DT |
denotes |
The |
T2097 |
694-706 |
NN |
denotes |
availability |
T2098 |
729-741 |
VBD |
denotes |
demonstrated |
T2099 |
707-709 |
IN |
denotes |
of |
T2100 |
710-720 |
JJ |
denotes |
homozygous |
T2101 |
721-728 |
NNS |
denotes |
animals |
T2102 |
742-746 |
IN |
denotes |
that |
T2103 |
815-819 |
VBD |
denotes |
were |
T2104 |
747-751 |
CC |
denotes |
both |
T2105 |
766-776 |
NN |
denotes |
expression |
T2106 |
752-760 |
NN |
denotes |
reporter |
T2107 |
761-765 |
NN |
denotes |
gene |
T2108 |
777-780 |
CC |
denotes |
and |
T2109 |
781-784 |
DT |
denotes |
the |
T2110 |
785-789 |
NN |
denotes |
site |
T2111 |
790-792 |
IN |
denotes |
of |
T2112 |
793-802 |
NN |
denotes |
transgene |
T2113 |
803-814 |
NN |
denotes |
integration |
T2114 |
820-835 |
RB |
denotes |
developmentally |
T2115 |
836-843 |
JJ |
denotes |
neutral |
T2116 |
843-844 |
. |
denotes |
. |
T1970 |
0-150 |
sentence |
denotes |
Two ECFP+ and two EYFP+ clones exhibiting robust fluorescence were used to generate chimeras through ES cell <-> morula stage embryo aggregation [13]. |
T1971 |
0-3 |
CD |
denotes |
Two |
T1972 |
4-9 |
JJ |
denotes |
ECFP+ |
T1973 |
24-30 |
NNS |
denotes |
clones |
T1974 |
10-13 |
CC |
denotes |
and |
T1975 |
14-17 |
CD |
denotes |
two |
T1976 |
18-23 |
JJ |
denotes |
EYFP+ |
T1977 |
67-71 |
VBN |
denotes |
used |
T1978 |
31-41 |
VBG |
denotes |
exhibiting |
T1979 |
42-48 |
JJ |
denotes |
robust |
T1980 |
49-61 |
NN |
denotes |
fluorescence |
T1981 |
62-66 |
VBD |
denotes |
were |
T1982 |
72-74 |
TO |
denotes |
to |
T1983 |
75-83 |
VB |
denotes |
generate |
T1984 |
84-92 |
NNS |
denotes |
chimeras |
T1985 |
93-100 |
IN |
denotes |
through |
T1986 |
101-103 |
NN |
denotes |
ES |
T1987 |
104-108 |
NN |
denotes |
cell |
T1988 |
109-110 |
SYM |
denotes |
< |
T1989 |
133-144 |
NN |
denotes |
aggregation |
T1990 |
110-111 |
HYPH |
denotes |
- |
T1991 |
111-112 |
SYM |
denotes |
> |
T1992 |
113-119 |
NN |
denotes |
morula |
T1993 |
120-125 |
NN |
denotes |
stage |
T1994 |
126-132 |
NN |
denotes |
embryo |
T1995 |
145-146 |
-LRB- |
denotes |
[ |
T1996 |
146-148 |
CD |
denotes |
13 |
T1997 |
148-149 |
-RRB- |
denotes |
] |
T1998 |
149-150 |
. |
denotes |
. |
T1999 |
150-423 |
sentence |
denotes |
All four ES cell lines transmitted through the germline, leading to the establishment of, CK4/ECFP and CK6/ECFP, two strains of mice having cyan fluorescent protein expression and, YC5/EYFP and 2A4/EYFP, two strains having widespread yellow fluorescent protein expression. |
T2000 |
151-154 |
DT |
denotes |
All |
T2001 |
168-173 |
NNS |
denotes |
lines |
T2002 |
155-159 |
CD |
denotes |
four |
T2041 |
348-349 |
HYPH |
denotes |
/ |
T2042 |
353-355 |
, |
denotes |
, |
T2043 |
355-358 |
CD |
denotes |
two |
T2044 |
359-366 |
NNS |
denotes |
strains |
T2045 |
367-373 |
VBG |
denotes |
having |
T2046 |
374-384 |
JJ |
denotes |
widespread |
T2047 |
412-422 |
NN |
denotes |
expression |
T2048 |
385-391 |
JJ |
denotes |
yellow |
T2049 |
404-411 |
NN |
denotes |
protein |
T2050 |
392-403 |
JJ |
denotes |
fluorescent |
T2051 |
422-423 |
. |
denotes |
. |
T2052 |
423-596 |
sentence |
denotes |
In these mice the respective FP expression is constituitive, starting at preimplantation stages and continuing through postimplantation embryogenesis to adulthood (Fig. 2). |
T2053 |
424-426 |
IN |
denotes |
In |
T2054 |
467-469 |
VBZ |
denotes |
is |
T2055 |
427-432 |
DT |
denotes |
these |
T2056 |
433-437 |
NNS |
denotes |
mice |
T2057 |
438-441 |
DT |
denotes |
the |
T2058 |
456-466 |
NN |
denotes |
expression |
T2059 |
442-452 |
JJ |
denotes |
respective |
T2060 |
453-455 |
NN |
denotes |
FP |
T2061 |
470-483 |
JJ |
denotes |
constituitive |
T2062 |
483-485 |
, |
denotes |
, |
T2063 |
485-493 |
VBG |
denotes |
starting |
T2064 |
494-496 |
IN |
denotes |
at |
T2065 |
497-512 |
NN |
denotes |
preimplantation |
T2066 |
513-519 |
NNS |
denotes |
stages |
T2067 |
520-523 |
CC |
denotes |
and |
T2068 |
524-534 |
VBG |
denotes |
continuing |
R1280 |
T2014 |
T2013 |
prep |
of,establishment |
R1281 |
T2015 |
T2014 |
punct |
", ",of |
R1282 |
T2016 |
T2017 |
compound |
CK4,ECFP |
R1283 |
T2017 |
T2014 |
pobj |
ECFP,of |
R1284 |
T2018 |
T2017 |
punct |
/,ECFP |
R1285 |
T2019 |
T2017 |
cc |
and,ECFP |
R1286 |
T2020 |
T2021 |
compound |
CK6,ECFP |
R1287 |
T2021 |
T2017 |
conj |
ECFP,ECFP |
R1288 |
T2022 |
T2021 |
punct |
/,ECFP |
R1289 |
T2023 |
T2017 |
punct |
", ",ECFP |
R1290 |
T2024 |
T2025 |
nummod |
two,strains |
R1291 |
T2025 |
T2017 |
appos |
strains,ECFP |
R1292 |
T2026 |
T2025 |
prep |
of,strains |
R1293 |
T2027 |
T2026 |
pobj |
mice,of |
R1294 |
T2028 |
T2025 |
acl |
having,strains |
R1295 |
T2029 |
T2030 |
nmod |
cyan,protein |
R1296 |
T2030 |
T2032 |
compound |
protein,expression |
R1297 |
T2031 |
T2030 |
amod |
fluorescent,protein |
R1298 |
T2032 |
T2028 |
dobj |
expression,having |
R1299 |
T2033 |
T2017 |
cc |
and,ECFP |
R1300 |
T2034 |
T2017 |
punct |
", ",ECFP |
R1301 |
T2035 |
T2036 |
compound |
YC5,EYFP |
R1302 |
T2036 |
T2017 |
conj |
EYFP,ECFP |
R1303 |
T2037 |
T2036 |
punct |
/,EYFP |
R1304 |
T2038 |
T2036 |
cc |
and,EYFP |
R1305 |
T2039 |
T2040 |
compound |
2A4,EYFP |
R1306 |
T2040 |
T2036 |
conj |
EYFP,EYFP |
R1307 |
T2041 |
T2040 |
punct |
/,EYFP |
R1308 |
T2042 |
T2036 |
punct |
", ",EYFP |
R1309 |
T2043 |
T2044 |
nummod |
two,strains |
R1310 |
T2044 |
T2036 |
appos |
strains,EYFP |
R1311 |
T2045 |
T2044 |
acl |
having,strains |
R1312 |
T2046 |
T2047 |
amod |
widespread,expression |
R1313 |
T2047 |
T2045 |
dobj |
expression,having |
R1314 |
T2048 |
T2049 |
amod |
yellow,protein |
R1315 |
T2049 |
T2047 |
compound |
protein,expression |
R1316 |
T2050 |
T2049 |
amod |
fluorescent,protein |
R1317 |
T2051 |
T2005 |
punct |
.,transmitted |
R1318 |
T2053 |
T2054 |
prep |
In,is |
R1319 |
T2055 |
T2056 |
det |
these,mice |
R1320 |
T2056 |
T2053 |
pobj |
mice,In |
R1321 |
T2057 |
T2058 |
det |
the,expression |
R1322 |
T2058 |
T2054 |
nsubj |
expression,is |
R1323 |
T2059 |
T2058 |
amod |
respective,expression |
R1324 |
T2060 |
T2058 |
compound |
FP,expression |
R1325 |
T2061 |
T2054 |
acomp |
constituitive,is |
R1326 |
T2062 |
T2054 |
punct |
", ",is |
R1327 |
T2063 |
T2054 |
advcl |
starting,is |
R1328 |
T2064 |
T2063 |
prep |
at,starting |
R1329 |
T2065 |
T2066 |
compound |
preimplantation,stages |
R1330 |
T2066 |
T2064 |
pobj |
stages,at |
R1331 |
T2067 |
T2063 |
cc |
and,starting |
R1332 |
T2068 |
T2063 |
conj |
continuing,starting |
R1333 |
T2069 |
T2068 |
prep |
through,continuing |
R1334 |
T2070 |
T2071 |
compound |
postimplantation,embryogenesis |
R1335 |
T2071 |
T2069 |
pobj |
embryogenesis,through |
R1336 |
T2072 |
T2068 |
prep |
to,continuing |
R1337 |
T2073 |
T2072 |
pobj |
adulthood,to |
R1338 |
T2074 |
T2075 |
punct |
(,Fig. |
R1339 |
T2075 |
T2054 |
parataxis |
Fig.,is |
R1340 |
T2076 |
T2075 |
nummod |
2,Fig. |
R1341 |
T2077 |
T2075 |
punct |
),Fig. |
R1342 |
T2078 |
T2054 |
punct |
.,is |
R1343 |
T2080 |
T2081 |
nummod |
One,line |
R1344 |
T2081 |
T2082 |
nsubjpass |
line,chosen |
R1345 |
T2083 |
T2081 |
acl |
expressing,line |
R1346 |
T2084 |
T2085 |
det |
each,color |
R1347 |
T2085 |
T2083 |
dobj |
color,expressing |
R1348 |
T2086 |
T2082 |
auxpass |
was,chosen |
R1349 |
T2087 |
T2082 |
prep |
for,chosen |
R1350 |
T2088 |
T2089 |
amod |
further,analysis |
R1351 |
T2089 |
T2087 |
pobj |
analysis,for |
R1352 |
T2090 |
T2089 |
cc |
and,analysis |
R1353 |
T2091 |
T2089 |
conj |
breeding,analysis |
R1354 |
T2092 |
T2091 |
prep |
to,breeding |
R1355 |
T2093 |
T2092 |
pobj |
homozygosity,to |
R1356 |
T2094 |
T2082 |
punct |
.,chosen |
R1357 |
T2096 |
T2097 |
det |
The,availability |
R1358 |
T2097 |
T2098 |
nsubj |
availability,demonstrated |
R1359 |
T2099 |
T2097 |
prep |
of,availability |
R1360 |
T2100 |
T2101 |
amod |
homozygous,animals |
R1361 |
T2101 |
T2099 |
pobj |
animals,of |
R1263 |
T1995 |
T1996 |
punct |
[,13 |
R1240 |
T1971 |
T1972 |
advmod |
Two,ECFP+ |
R1241 |
T1972 |
T1973 |
amod |
ECFP+,clones |
R1242 |
T1973 |
T1977 |
nsubjpass |
clones,used |
R1243 |
T1974 |
T1972 |
cc |
and,ECFP+ |
R1244 |
T1975 |
T1976 |
advmod |
two,EYFP+ |
R1245 |
T1976 |
T1972 |
conj |
EYFP+,ECFP+ |
R1246 |
T1978 |
T1973 |
acl |
exhibiting,clones |
R1247 |
T1979 |
T1980 |
amod |
robust,fluorescence |
R1248 |
T1980 |
T1978 |
dobj |
fluorescence,exhibiting |
R1249 |
T1981 |
T1977 |
auxpass |
were,used |
R1250 |
T1982 |
T1983 |
aux |
to,generate |
R1251 |
T1983 |
T1977 |
advcl |
generate,used |
R1252 |
T1984 |
T1983 |
dobj |
chimeras,generate |
R1253 |
T1985 |
T1983 |
prep |
through,generate |
R1254 |
T1986 |
T1987 |
compound |
ES,cell |
R1255 |
T1987 |
T1985 |
pobj |
cell,through |
R1256 |
T1988 |
T1989 |
punct |
<,aggregation |
R1257 |
T1989 |
T1987 |
prep |
aggregation,cell |
R1258 |
T1990 |
T1989 |
punct |
-,aggregation |
R1259 |
T1991 |
T1989 |
punct |
>,aggregation |
R1260 |
T1992 |
T1993 |
compound |
morula,stage |
R1261 |
T1993 |
T1989 |
compound |
stage,aggregation |
R1262 |
T1994 |
T1989 |
compound |
embryo,aggregation |
R1264 |
T1996 |
T1977 |
parataxis |
13,used |
R1265 |
T1997 |
T1996 |
punct |
],13 |
R1266 |
T1998 |
T1977 |
punct |
.,used |
R1267 |
T2000 |
T2001 |
det |
All,lines |
R1268 |
T2001 |
T2005 |
nsubj |
lines,transmitted |
R1269 |
T2002 |
T2001 |
nummod |
four,lines |
R1270 |
T2003 |
T2001 |
compound |
ES,lines |
R1271 |
T2004 |
T2001 |
compound |
cell,lines |
R1272 |
T2006 |
T2005 |
prep |
through,transmitted |
R1273 |
T2007 |
T2008 |
det |
the,germline |
R1274 |
T2008 |
T2006 |
pobj |
germline,through |
R1275 |
T2009 |
T2005 |
punct |
", ",transmitted |
R1276 |
T2010 |
T2005 |
advcl |
leading,transmitted |
R1277 |
T2011 |
T2010 |
prep |
to,leading |
R1278 |
T2012 |
T2013 |
det |
the,establishment |
R1279 |
T2013 |
T2011 |
pobj |
establishment,to |
R1362 |
T2102 |
T2103 |
mark |
that,were |
R1363 |
T2103 |
T2098 |
ccomp |
were,demonstrated |
R1364 |
T2104 |
T2105 |
preconj |
both,expression |
R1365 |
T2105 |
T2103 |
nsubj |
expression,were |
R1366 |
T2106 |
T2107 |
compound |
reporter,gene |
R1367 |
T2107 |
T2105 |
compound |
gene,expression |
R1368 |
T2108 |
T2105 |
cc |
and,expression |
R1369 |
T2109 |
T2110 |
det |
the,site |
R1370 |
T2110 |
T2105 |
conj |
site,expression |
R1371 |
T2111 |
T2110 |
prep |
of,site |
R1372 |
T2112 |
T2113 |
compound |
transgene,integration |
R1373 |
T2113 |
T2111 |
pobj |
integration,of |
R1374 |
T2114 |
T2115 |
advmod |
developmentally,neutral |
R1375 |
T2115 |
T2103 |
acomp |
neutral,were |
R1376 |
T2116 |
T2098 |
punct |
.,demonstrated |