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PMC:1140370 / 8635-9610 JSONTXT

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craft-sa-dev

Id Subject Object Predicate Lexical cue
T2629 0-3 NN denotes DNA
T2630 4-12 NN denotes helicase
T2631 27-33 NNS denotes assays
T2632 13-16 CC denotes and
T2633 17-20 NN denotes gel
T2634 21-26 NN denotes shift
T2635 33-303 sentence denotes In gel shift assays, Mcm4/6/7 proteins were incubated at 30°C for 30 min in reaction mixtures (15 μl) containing 25 mM HEPES–NaOH (pH 7.5), 50 mM sodium acetate, 10 mM Mg(CH3COO)2, 1 mM DTT, 0.25 μg/ml BSA, 0.5 mM ATP-γ-S and labeled substrates of the amount indicated.
T2636 34-36 IN denotes In
T2637 78-87 VBN denotes incubated
T2638 37-40 NN denotes gel
T2639 41-46 NN denotes shift
T2640 47-53 NNS denotes assays
T2641 53-55 , denotes ,
T2642 55-59 NN denotes Mcm4
T2643 64-72 NN denotes proteins
T2644 59-60 HYPH denotes /
T2645 60-61 CD denotes 6
T2646 61-62 HYPH denotes /
T2647 62-63 CD denotes 7
T2648 73-77 VBD denotes were
T2649 88-90 IN denotes at
T2650 91-93 CD denotes 30
T2651 93-95 NN denotes °C
T2652 96-99 IN denotes for
T2653 100-102 CD denotes 30
T2654 103-106 NN denotes min
T2655 107-109 IN denotes in
T2656 110-118 NN denotes reaction
T2657 119-127 NNS denotes mixtures
T2658 128-129 -LRB- denotes (
T2659 132-134 NN denotes μl
T2660 129-131 CD denotes 15
T2661 134-135 -RRB- denotes )
T2662 136-146 VBG denotes containing
T2663 147-149 CD denotes 25
T2664 150-152 NN denotes mM
T2665 159-163 NN denotes NaOH
T2666 153-158 NN denotes HEPES
T2667 158-159 HYPH denotes –
T2668 164-165 -LRB- denotes (
T2669 165-167 NN denotes pH
T2670 168-171 CD denotes 7.5
T2671 171-172 -RRB- denotes )
T2672 172-174 , denotes ,
T2673 174-176 CD denotes 50
T2674 177-179 NN denotes mM
T2675 187-194 NN denotes acetate
T2676 180-186 NN denotes sodium
T2677 194-196 , denotes ,
T2678 196-198 CD denotes 10
T2679 199-201 NN denotes mM
T2680 205-211 NN denotes CH3COO
T2681 202-204 NN denotes Mg
T2682 204-205 -LRB- denotes (
T2683 211-212 -RRB- denotes )
T2684 212-213 CD denotes 2
T2685 213-215 , denotes ,
T2686 215-216 CD denotes 1
T2687 217-219 NN denotes mM
T2688 220-223 NN denotes DTT
T2689 223-225 , denotes ,
T2690 225-229 CD denotes 0.25
T2691 230-232 NN denotes μg
T2692 236-239 NN denotes BSA
T2693 232-233 SYM denotes /
T2694 233-235 NN denotes ml
T2695 239-241 , denotes ,
T2696 241-244 CD denotes 0.5
T2697 245-247 NN denotes mM
T2698 254-255 NN denotes S
T2699 248-251 NN denotes ATP
T2700 251-252 HYPH denotes -
T2701 252-253 SYM denotes γ
T2702 253-254 HYPH denotes -
T2703 256-259 CC denotes and
T2704 260-267 VBN denotes labeled
T2705 268-278 NNS denotes substrates
T2706 279-281 IN denotes of
T2707 282-285 DT denotes the
T2708 286-292 NN denotes amount
T2709 293-302 VBN denotes indicated
T2710 302-303 . denotes .
T2711 303-529 sentence denotes After addition of 2 μl of 50% glycerol, the reaction mixtures were directly applied on a polyacrylamide gel containing 6 mM magnesium acetate and 5% glycerol in 0.5× TBE, and electrophoresis was conducted at room temperature.
T2712 304-309 IN denotes After
T2713 380-387 VBN denotes applied
T2714 310-318 NN denotes addition
T2715 319-321 IN denotes of
T2716 322-323 CD denotes 2
T2717 324-326 NN denotes μl
T2718 327-329 IN denotes of
T2719 330-332 CD denotes 50
T2720 332-333 NN denotes %
T2721 334-342 NN denotes glycerol
T2722 342-344 , denotes ,
T2723 344-347 DT denotes the
T2724 357-365 NNS denotes mixtures
T2725 348-356 NN denotes reaction
T2726 366-370 VBD denotes were
T2727 371-379 RB denotes directly
T2728 388-390 IN denotes on
T2729 391-392 DT denotes a
T2730 408-411 NN denotes gel
T2731 393-407 NN denotes polyacrylamide
T2732 412-422 VBG denotes containing
T2733 423-424 CD denotes 6
T2734 425-427 NN denotes mM
T2735 438-445 NN denotes acetate
T2736 428-437 NN denotes magnesium
T2737 446-449 CC denotes and
T2738 450-451 CD denotes 5
T2739 451-452 NN denotes %
T2740 453-461 NN denotes glycerol
T2741 462-464 IN denotes in
T2742 465-468 CD denotes 0.5
T2743 470-473 NN denotes TBE
T2744 468-469 SYM denotes ×
T2745 473-475 , denotes ,
T2746 475-478 CC denotes and
T2747 479-494 NN denotes electrophoresis
T2748 499-508 VBN denotes conducted
T2749 495-498 VBD denotes was
T2750 509-511 IN denotes at
T2751 512-516 NN denotes room
T2752 517-528 NN denotes temperature
T2753 528-529 . denotes .
T2754 529-747 sentence denotes For DNA helicase assays, the reaction mixtures of the gel shift assays, as described above, were incubated at 30°C for 15 min, and then the ATP was added at 10 mM, followed by incubation at 37°C for additional 30 min.
T2755 530-533 IN denotes For
T2756 627-636 VBN denotes incubated
T2757 534-537 NN denotes DNA
T2758 547-553 NNS denotes assays
T2759 538-546 NN denotes helicase
T2760 553-555 , denotes ,
T2761 555-558 DT denotes the
T2762 568-576 NNS denotes mixtures
T2763 559-567 NN denotes reaction
T2764 577-579 IN denotes of
T2765 580-583 DT denotes the
T2766 594-600 NNS denotes assays
T2767 584-587 NN denotes gel
T2768 588-593 NN denotes shift
T2769 600-602 , denotes ,
T2770 602-604 IN denotes as
T2771 605-614 VBN denotes described
T2772 615-620 RB denotes above
T2773 620-622 , denotes ,
T2774 622-626 VBD denotes were
T2775 637-639 IN denotes at
T2776 640-642 CD denotes 30
T2777 642-644 NN denotes °C
T2778 645-648 IN denotes for
T2779 649-651 CD denotes 15
T2780 652-655 NN denotes min
T2781 655-657 , denotes ,
T2782 657-660 CC denotes and
T2783 661-665 RB denotes then
T2784 666-669 DT denotes the
T2785 670-673 NN denotes ATP
T2786 678-683 VBN denotes added
T2787 674-677 VBD denotes was
T2788 684-686 IN denotes at
T2789 687-689 CD denotes 10
T2790 690-692 NN denotes mM
T2791 692-694 , denotes ,
T2792 694-702 VBN denotes followed
T2793 703-705 IN denotes by
T2794 706-716 NN denotes incubation
T2795 717-719 IN denotes at
T2796 720-722 CD denotes 37
T2797 722-724 NN denotes °C
T2798 725-728 IN denotes for
T2799 729-739 JJ denotes additional
T2800 743-746 NN denotes min
T2801 740-742 CD denotes 30
T2802 746-747 . denotes .
T2803 747-882 sentence denotes The reactions were terminated by addition of EDTA (20 mM), SDS (0.1%) and 2 μg proteinase K, and were incubated for additional 15 min.
T2804 748-751 DT denotes The
T2805 752-761 NNS denotes reactions
T2806 767-777 VBN denotes terminated
T2807 762-766 VBD denotes were
T2808 778-780 IN denotes by
T2809 781-789 NN denotes addition
T2810 790-792 IN denotes of
T2811 793-797 NN denotes EDTA
T2812 798-799 -LRB- denotes (
T2813 802-804 NN denotes mM
T2814 799-801 CD denotes 20
T2815 804-805 -RRB- denotes )
T2816 805-807 , denotes ,
T2817 807-810 NN denotes SDS
T2818 811-812 -LRB- denotes (
T2819 815-816 NN denotes %
T2820 812-815 CD denotes 0.1
T2821 816-817 -RRB- denotes )
T2822 818-821 CC denotes and
T2823 822-823 CD denotes 2
T2824 824-826 NN denotes μg
T2825 838-839 NN denotes K
T2826 827-837 NN denotes proteinase
T2827 839-841 , denotes ,
T2828 841-844 CC denotes and
T2829 845-849 VBD denotes were
T2830 850-859 VBN denotes incubated
T2831 860-863 IN denotes for
T2832 864-874 JJ denotes additional
T2833 878-881 NN denotes min
T2834 875-877 CD denotes 15
T2835 881-882 . denotes .
T2836 882-975 sentence denotes The samples were separated by electrophoresis on nondenaturing polyacrylamide gel in 1× TBE.
T2837 883-886 DT denotes The
T2838 887-894 NNS denotes samples
T2839 900-909 VBN denotes separated
T2840 895-899 VBD denotes were
T2841 910-912 IN denotes by
T2842 913-928 NN denotes electrophoresis
T2843 929-931 IN denotes on
T2844 932-945 JJ denotes nondenaturing
T2845 961-964 NN denotes gel
T2846 946-960 NN denotes polyacrylamide
T2847 965-967 IN denotes in
T2848 968-969 CD denotes 1
T2849 971-974 NN denotes TBE
T2850 969-970 SYM denotes ×
T2851 974-975 . denotes .
R1668 T2629 T2630 nmod DNA,helicase
R1669 T2630 T2631 nmod helicase,assays
R1670 T2632 T2630 cc and,helicase
R1671 T2633 T2634 compound gel,shift
R1672 T2634 T2630 conj shift,helicase
R1673 T2636 T2637 prep In,incubated
R1674 T2638 T2639 compound gel,shift
R1675 T2639 T2640 compound shift,assays
R1676 T2640 T2636 pobj assays,In
R1677 T2641 T2637 punct ", ",incubated
R1678 T2642 T2643 nmod Mcm4,proteins
R1679 T2643 T2637 nsubjpass proteins,incubated
R1680 T2644 T2642 punct /,Mcm4
R1681 T2645 T2642 nummod 6,Mcm4
R1682 T2646 T2642 punct /,Mcm4
R1683 T2647 T2642 nummod 7,Mcm4
R1684 T2648 T2637 auxpass were,incubated
R1685 T2649 T2637 prep at,incubated
R1686 T2650 T2651 nummod 30,°C
R1687 T2651 T2649 pobj °C,at
R1688 T2652 T2637 prep for,incubated
R1689 T2653 T2654 nummod 30,min
R1690 T2654 T2652 pobj min,for
R1691 T2655 T2637 prep in,incubated
R1692 T2656 T2657 compound reaction,mixtures
R1693 T2657 T2655 pobj mixtures,in
R1694 T2658 T2659 punct (,μl
R1695 T2659 T2657 parataxis μl,mixtures
R1696 T2660 T2659 nummod 15,μl
R1697 T2661 T2659 punct ),μl
R1698 T2662 T2657 acl containing,mixtures
R1699 T2663 T2664 nummod 25,mM
R1700 T2664 T2665 compound mM,NaOH
R1701 T2665 T2662 dobj NaOH,containing
R1702 T2666 T2665 compound HEPES,NaOH
R1703 T2667 T2665 punct –,NaOH
R1704 T2668 T2669 punct (,pH
R1705 T2669 T2665 parataxis pH,NaOH
R1706 T2670 T2669 nummod 7.5,pH
R1707 T2671 T2669 punct ),pH
R1708 T2672 T2665 punct ", ",NaOH
R1709 T2673 T2674 nummod 50,mM
R1710 T2674 T2675 compound mM,acetate
R1711 T2675 T2665 conj acetate,NaOH
R1712 T2676 T2675 compound sodium,acetate
R1713 T2677 T2675 punct ", ",acetate
R1714 T2678 T2679 nummod 10,mM
R1715 T2679 T2680 nmod mM,CH3COO
R1716 T2680 T2675 conj CH3COO,acetate
R1717 T2681 T2680 nmod Mg,CH3COO
R1718 T2682 T2680 punct (,CH3COO
R1719 T2683 T2680 punct ),CH3COO
R1720 T2684 T2680 nummod 2,CH3COO
R1721 T2685 T2680 punct ", ",CH3COO
R1722 T2686 T2687 nummod 1,mM
R1723 T2687 T2688 compound mM,DTT
R1724 T2688 T2680 conj DTT,CH3COO
R1725 T2689 T2688 punct ", ",DTT
R1726 T2690 T2691 nummod 0.25,μg
R1727 T2691 T2692 nmod μg,BSA
R1728 T2692 T2688 conj BSA,DTT
R1729 T2693 T2694 punct /,ml
R1730 T2694 T2691 prep ml,μg
R1731 T2695 T2692 punct ", ",BSA
R1732 T2696 T2697 nummod 0.5,mM
R1733 T2697 T2698 nmod mM,S
R1734 T2698 T2692 conj S,BSA
R1735 T2699 T2698 nmod ATP,S
R1736 T2700 T2698 punct -,S
R1737 T2701 T2698 punct γ,S
R1738 T2702 T2698 punct -,S
R1739 T2703 T2698 cc and,S
R1740 T2704 T2705 amod labeled,substrates
R1741 T2705 T2698 conj substrates,S
R1742 T2706 T2705 prep of,substrates
R1743 T2707 T2708 det the,amount
R1744 T2708 T2706 pobj amount,of
R1745 T2709 T2708 acl indicated,amount
R1746 T2710 T2637 punct .,incubated
R1747 T2712 T2713 prep After,applied
R1748 T2714 T2712 pobj addition,After
R1749 T2715 T2714 prep of,addition
R1750 T2716 T2717 nummod 2,μl
R1751 T2717 T2715 pobj μl,of
R1752 T2718 T2717 prep of,μl
R1753 T2719 T2720 nummod 50,%
R1754 T2720 T2721 compound %,glycerol
R1755 T2721 T2718 pobj glycerol,of
R1756 T2722 T2713 punct ", ",applied
R1757 T2723 T2724 det the,mixtures
R1758 T2724 T2713 nsubjpass mixtures,applied
R1759 T2725 T2724 compound reaction,mixtures
R1760 T2726 T2713 auxpass were,applied
R1761 T2727 T2713 advmod directly,applied
R1762 T2728 T2713 prep on,applied
R1763 T2729 T2730 det a,gel
R1764 T2730 T2728 pobj gel,on
R1765 T2731 T2730 compound polyacrylamide,gel
R1766 T2732 T2730 acl containing,gel
R1767 T2733 T2734 nummod 6,mM
R1768 T2734 T2735 compound mM,acetate
R1769 T2735 T2732 dobj acetate,containing
R1770 T2736 T2735 compound magnesium,acetate
R1771 T2737 T2735 cc and,acetate
R1772 T2738 T2739 nummod 5,%
R1773 T2739 T2740 compound %,glycerol
R1774 T2740 T2735 conj glycerol,acetate
R1775 T2741 T2732 prep in,containing
R1776 T2742 T2743 nummod 0.5,TBE
R1777 T2743 T2741 pobj TBE,in
R1778 T2744 T2742 punct ×,0.5
R1779 T2745 T2713 punct ", ",applied
R1780 T2746 T2713 cc and,applied
R1781 T2747 T2748 nsubjpass electrophoresis,conducted
R1782 T2748 T2713 conj conducted,applied
R1783 T2749 T2748 auxpass was,conducted
R1784 T2750 T2748 prep at,conducted
R1785 T2751 T2752 compound room,temperature
R1786 T2752 T2750 pobj temperature,at
R1787 T2753 T2748 punct .,conducted
R1788 T2755 T2756 prep For,incubated
R1789 T2757 T2758 compound DNA,assays
R1790 T2758 T2755 pobj assays,For
R1791 T2759 T2758 compound helicase,assays
R1792 T2760 T2756 punct ", ",incubated
R1793 T2761 T2762 det the,mixtures
R1794 T2762 T2756 nsubjpass mixtures,incubated
R1795 T2763 T2762 compound reaction,mixtures
R1796 T2764 T2762 prep of,mixtures
R1797 T2765 T2766 det the,assays
R1798 T2766 T2764 pobj assays,of
R1799 T2767 T2768 compound gel,shift
R1800 T2768 T2766 compound shift,assays
R1801 T2769 T2756 punct ", ",incubated
R1802 T2770 T2771 mark as,described
R1803 T2771 T2756 advcl described,incubated
R1804 T2772 T2771 advmod above,described
R1805 T2773 T2756 punct ", ",incubated
R1806 T2774 T2756 auxpass were,incubated
R1807 T2775 T2756 prep at,incubated
R1808 T2776 T2777 nummod 30,°C
R1809 T2777 T2775 pobj °C,at
R1810 T2778 T2756 prep for,incubated
R1811 T2779 T2780 nummod 15,min
R1812 T2780 T2778 pobj min,for
R1813 T2781 T2756 punct ", ",incubated
R1814 T2782 T2756 cc and,incubated
R1815 T2783 T2782 advmod then,and
R1816 T2784 T2785 det the,ATP
R1817 T2785 T2786 nsubjpass ATP,added
R1818 T2786 T2756 conj added,incubated
R1819 T2787 T2786 auxpass was,added
R1820 T2788 T2786 prep at,added
R1821 T2789 T2790 nummod 10,mM
R1822 T2790 T2788 pobj mM,at
R1823 T2791 T2786 punct ", ",added
R1824 T2792 T2786 advcl followed,added
R1825 T2793 T2792 agent by,followed
R1826 T2794 T2793 pobj incubation,by
R1827 T2795 T2794 prep at,incubation
R1828 T2796 T2797 nummod 37,°C
R1829 T2797 T2795 pobj °C,at
R1830 T2798 T2794 prep for,incubation
R1831 T2799 T2800 amod additional,min
R1832 T2800 T2798 pobj min,for
R1833 T2801 T2800 nummod 30,min
R1834 T2802 T2786 punct .,added
R1835 T2804 T2805 det The,reactions
R1836 T2805 T2806 nsubjpass reactions,terminated
R1837 T2807 T2806 auxpass were,terminated
R1838 T2808 T2806 agent by,terminated
R1839 T2809 T2808 pobj addition,by
R1840 T2810 T2809 prep of,addition
R1841 T2811 T2810 pobj EDTA,of
R1842 T2812 T2813 punct (,mM
R1843 T2813 T2811 parataxis mM,EDTA
R1844 T2814 T2813 nummod 20,mM
R1845 T2815 T2813 punct ),mM
R1846 T2816 T2811 punct ", ",EDTA
R1847 T2817 T2811 conj SDS,EDTA
R1848 T2818 T2819 punct (,%
R1849 T2819 T2817 parataxis %,SDS
R1850 T2820 T2819 nummod 0.1,%
R1851 T2821 T2819 punct ),%
R1852 T2822 T2817 cc and,SDS
R1853 T2823 T2824 nummod 2,μg
R1854 T2824 T2825 compound μg,K
R1855 T2825 T2817 conj K,SDS
R1856 T2826 T2825 compound proteinase,K
R1857 T2827 T2806 punct ", ",terminated
R1858 T2828 T2806 cc and,terminated
R1859 T2829 T2830 auxpass were,incubated
R1860 T2830 T2806 conj incubated,terminated
R1861 T2831 T2830 prep for,incubated
R1862 T2832 T2833 amod additional,min
R1863 T2833 T2831 pobj min,for
R1864 T2834 T2833 nummod 15,min
R1865 T2835 T2806 punct .,terminated
R1866 T2837 T2838 det The,samples
R1867 T2838 T2839 nsubjpass samples,separated
R1868 T2840 T2839 auxpass were,separated
R1869 T2841 T2839 prep by,separated
R1870 T2842 T2841 pobj electrophoresis,by
R1871 T2843 T2839 prep on,separated
R1872 T2844 T2845 amod nondenaturing,gel
R1873 T2845 T2843 pobj gel,on
R1874 T2846 T2845 compound polyacrylamide,gel
R1875 T2847 T2845 prep in,gel
R1876 T2848 T2849 nummod 1,TBE
R1877 T2849 T2847 pobj TBE,in
R1878 T2850 T2848 punct ×,1
R1879 T2851 T2839 punct .,separated

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T2602 0-3 CHEBI_SO_EXT:DNA denotes DNA
T2603 0-12 GO_EXT:0003678 denotes DNA helicase
T2604 55-59 PR_EXT:000010246 denotes Mcm4
T2605 55-63 GO:0097373 denotes Mcm4/6/7
T2606 64-72 CHEBI_PR_EXT:protein denotes proteins
T2607 119-127 CHEBI:60004 denotes mixtures
T2608 153-158 CHEBI:46756 denotes HEPES
T2609 159-163 CHEBI:32145 denotes NaOH
T2610 180-194 CHEBI:32954 denotes sodium acetate
T2611 202-213 CHEBI:62964 denotes Mg(CH3COO)2
T2612 220-223 CHEBI:18320 denotes DTT
T2613 248-255 CHEBI:27575 denotes ATP-γ-S
T2614 260-267 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes labeled
T2615 334-342 CHEBI:17754 denotes glycerol
T2616 357-365 CHEBI:60004 denotes mixtures
T2617 393-407 CHEBI_EXT:polyacrylamide denotes polyacrylamide
T2618 428-445 CHEBI:62964 denotes magnesium acetate
T2619 453-461 CHEBI:17754 denotes glycerol
T2620 534-537 CHEBI_SO_EXT:DNA denotes DNA
T2621 534-546 GO_EXT:0003678 denotes DNA helicase
T2622 568-576 CHEBI:60004 denotes mixtures
T2623 670-673 CHEBI_EXT:ATP denotes ATP
T2624 793-797 CHEBI_EXT:EDTA denotes EDTA
T2625 807-810 CHEBI:8984 denotes SDS
T2626 827-837 GO_EXT:0008233 denotes proteinase
T2627 935-945 GO_EXT:macromolecule_denaturation denotes denaturing
T2628 946-960 CHEBI_EXT:polyacrylamide denotes polyacrylamide

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T2587 55-59 PR:000010246 denotes Mcm4
T2588 55-63 GO:0097373 denotes Mcm4/6/7
T2589 119-127 CHEBI:60004 denotes mixtures
T2590 153-158 CHEBI:46756 denotes HEPES
T2591 159-163 CHEBI:32145 denotes NaOH
T2592 180-194 CHEBI:32954 denotes sodium acetate
T2593 202-213 CHEBI:62964 denotes Mg(CH3COO)2
T2594 220-223 CHEBI:18320 denotes DTT
T2595 248-255 CHEBI:27575 denotes ATP-γ-S
T2596 334-342 CHEBI:17754 denotes glycerol
T2597 357-365 CHEBI:60004 denotes mixtures
T2598 428-445 CHEBI:62964 denotes magnesium acetate
T2599 453-461 CHEBI:17754 denotes glycerol
T2600 568-576 CHEBI:60004 denotes mixtures
T2601 807-810 CHEBI:8984 denotes SDS