Id |
Subject |
Object |
Predicate |
Lexical cue |
T2629 |
0-3 |
NN |
denotes |
DNA |
T2630 |
4-12 |
NN |
denotes |
helicase |
T2631 |
27-33 |
NNS |
denotes |
assays |
T2632 |
13-16 |
CC |
denotes |
and |
T2633 |
17-20 |
NN |
denotes |
gel |
T2634 |
21-26 |
NN |
denotes |
shift |
T2635 |
33-303 |
sentence |
denotes |
In gel shift assays, Mcm4/6/7 proteins were incubated at 30°C for 30 min in reaction mixtures (15 μl) containing 25 mM HEPES–NaOH (pH 7.5), 50 mM sodium acetate, 10 mM Mg(CH3COO)2, 1 mM DTT, 0.25 μg/ml BSA, 0.5 mM ATP-γ-S and labeled substrates of the amount indicated. |
T2636 |
34-36 |
IN |
denotes |
In |
T2637 |
78-87 |
VBN |
denotes |
incubated |
T2638 |
37-40 |
NN |
denotes |
gel |
T2639 |
41-46 |
NN |
denotes |
shift |
T2640 |
47-53 |
NNS |
denotes |
assays |
T2641 |
53-55 |
, |
denotes |
, |
T2642 |
55-59 |
NN |
denotes |
Mcm4 |
T2643 |
64-72 |
NN |
denotes |
proteins |
T2644 |
59-60 |
HYPH |
denotes |
/ |
T2645 |
60-61 |
CD |
denotes |
6 |
T2646 |
61-62 |
HYPH |
denotes |
/ |
T2647 |
62-63 |
CD |
denotes |
7 |
T2648 |
73-77 |
VBD |
denotes |
were |
T2649 |
88-90 |
IN |
denotes |
at |
T2650 |
91-93 |
CD |
denotes |
30 |
T2651 |
93-95 |
NN |
denotes |
°C |
T2652 |
96-99 |
IN |
denotes |
for |
T2653 |
100-102 |
CD |
denotes |
30 |
T2654 |
103-106 |
NN |
denotes |
min |
T2655 |
107-109 |
IN |
denotes |
in |
T2656 |
110-118 |
NN |
denotes |
reaction |
T2657 |
119-127 |
NNS |
denotes |
mixtures |
T2658 |
128-129 |
-LRB- |
denotes |
( |
T2659 |
132-134 |
NN |
denotes |
μl |
T2660 |
129-131 |
CD |
denotes |
15 |
T2661 |
134-135 |
-RRB- |
denotes |
) |
T2662 |
136-146 |
VBG |
denotes |
containing |
T2663 |
147-149 |
CD |
denotes |
25 |
T2664 |
150-152 |
NN |
denotes |
mM |
T2665 |
159-163 |
NN |
denotes |
NaOH |
T2666 |
153-158 |
NN |
denotes |
HEPES |
T2667 |
158-159 |
HYPH |
denotes |
– |
T2668 |
164-165 |
-LRB- |
denotes |
( |
T2669 |
165-167 |
NN |
denotes |
pH |
T2670 |
168-171 |
CD |
denotes |
7.5 |
T2671 |
171-172 |
-RRB- |
denotes |
) |
T2672 |
172-174 |
, |
denotes |
, |
T2673 |
174-176 |
CD |
denotes |
50 |
T2674 |
177-179 |
NN |
denotes |
mM |
T2675 |
187-194 |
NN |
denotes |
acetate |
T2676 |
180-186 |
NN |
denotes |
sodium |
T2677 |
194-196 |
, |
denotes |
, |
T2678 |
196-198 |
CD |
denotes |
10 |
T2679 |
199-201 |
NN |
denotes |
mM |
T2680 |
205-211 |
NN |
denotes |
CH3COO |
T2681 |
202-204 |
NN |
denotes |
Mg |
T2682 |
204-205 |
-LRB- |
denotes |
( |
T2683 |
211-212 |
-RRB- |
denotes |
) |
T2684 |
212-213 |
CD |
denotes |
2 |
T2685 |
213-215 |
, |
denotes |
, |
T2686 |
215-216 |
CD |
denotes |
1 |
T2687 |
217-219 |
NN |
denotes |
mM |
T2688 |
220-223 |
NN |
denotes |
DTT |
T2689 |
223-225 |
, |
denotes |
, |
T2690 |
225-229 |
CD |
denotes |
0.25 |
T2691 |
230-232 |
NN |
denotes |
μg |
T2692 |
236-239 |
NN |
denotes |
BSA |
T2693 |
232-233 |
SYM |
denotes |
/ |
T2694 |
233-235 |
NN |
denotes |
ml |
T2695 |
239-241 |
, |
denotes |
, |
T2696 |
241-244 |
CD |
denotes |
0.5 |
T2697 |
245-247 |
NN |
denotes |
mM |
T2698 |
254-255 |
NN |
denotes |
S |
T2699 |
248-251 |
NN |
denotes |
ATP |
T2700 |
251-252 |
HYPH |
denotes |
- |
T2701 |
252-253 |
SYM |
denotes |
γ |
T2702 |
253-254 |
HYPH |
denotes |
- |
T2703 |
256-259 |
CC |
denotes |
and |
T2704 |
260-267 |
VBN |
denotes |
labeled |
T2705 |
268-278 |
NNS |
denotes |
substrates |
T2706 |
279-281 |
IN |
denotes |
of |
T2707 |
282-285 |
DT |
denotes |
the |
T2708 |
286-292 |
NN |
denotes |
amount |
T2709 |
293-302 |
VBN |
denotes |
indicated |
T2710 |
302-303 |
. |
denotes |
. |
T2711 |
303-529 |
sentence |
denotes |
After addition of 2 μl of 50% glycerol, the reaction mixtures were directly applied on a polyacrylamide gel containing 6 mM magnesium acetate and 5% glycerol in 0.5× TBE, and electrophoresis was conducted at room temperature. |
T2712 |
304-309 |
IN |
denotes |
After |
T2713 |
380-387 |
VBN |
denotes |
applied |
T2714 |
310-318 |
NN |
denotes |
addition |
T2715 |
319-321 |
IN |
denotes |
of |
T2716 |
322-323 |
CD |
denotes |
2 |
T2717 |
324-326 |
NN |
denotes |
μl |
T2718 |
327-329 |
IN |
denotes |
of |
T2719 |
330-332 |
CD |
denotes |
50 |
T2720 |
332-333 |
NN |
denotes |
% |
T2721 |
334-342 |
NN |
denotes |
glycerol |
T2722 |
342-344 |
, |
denotes |
, |
T2723 |
344-347 |
DT |
denotes |
the |
T2724 |
357-365 |
NNS |
denotes |
mixtures |
T2725 |
348-356 |
NN |
denotes |
reaction |
T2726 |
366-370 |
VBD |
denotes |
were |
T2727 |
371-379 |
RB |
denotes |
directly |
T2728 |
388-390 |
IN |
denotes |
on |
T2729 |
391-392 |
DT |
denotes |
a |
T2730 |
408-411 |
NN |
denotes |
gel |
T2731 |
393-407 |
NN |
denotes |
polyacrylamide |
T2732 |
412-422 |
VBG |
denotes |
containing |
T2733 |
423-424 |
CD |
denotes |
6 |
T2734 |
425-427 |
NN |
denotes |
mM |
T2735 |
438-445 |
NN |
denotes |
acetate |
T2736 |
428-437 |
NN |
denotes |
magnesium |
T2737 |
446-449 |
CC |
denotes |
and |
T2738 |
450-451 |
CD |
denotes |
5 |
T2739 |
451-452 |
NN |
denotes |
% |
T2740 |
453-461 |
NN |
denotes |
glycerol |
T2741 |
462-464 |
IN |
denotes |
in |
T2742 |
465-468 |
CD |
denotes |
0.5 |
T2743 |
470-473 |
NN |
denotes |
TBE |
T2744 |
468-469 |
SYM |
denotes |
× |
T2745 |
473-475 |
, |
denotes |
, |
T2746 |
475-478 |
CC |
denotes |
and |
T2747 |
479-494 |
NN |
denotes |
electrophoresis |
T2748 |
499-508 |
VBN |
denotes |
conducted |
T2749 |
495-498 |
VBD |
denotes |
was |
T2750 |
509-511 |
IN |
denotes |
at |
T2751 |
512-516 |
NN |
denotes |
room |
T2752 |
517-528 |
NN |
denotes |
temperature |
T2753 |
528-529 |
. |
denotes |
. |
T2754 |
529-747 |
sentence |
denotes |
For DNA helicase assays, the reaction mixtures of the gel shift assays, as described above, were incubated at 30°C for 15 min, and then the ATP was added at 10 mM, followed by incubation at 37°C for additional 30 min. |
T2755 |
530-533 |
IN |
denotes |
For |
T2756 |
627-636 |
VBN |
denotes |
incubated |
T2757 |
534-537 |
NN |
denotes |
DNA |
T2758 |
547-553 |
NNS |
denotes |
assays |
T2759 |
538-546 |
NN |
denotes |
helicase |
T2760 |
553-555 |
, |
denotes |
, |
T2761 |
555-558 |
DT |
denotes |
the |
T2762 |
568-576 |
NNS |
denotes |
mixtures |
T2763 |
559-567 |
NN |
denotes |
reaction |
T2764 |
577-579 |
IN |
denotes |
of |
T2765 |
580-583 |
DT |
denotes |
the |
T2766 |
594-600 |
NNS |
denotes |
assays |
T2767 |
584-587 |
NN |
denotes |
gel |
T2768 |
588-593 |
NN |
denotes |
shift |
T2769 |
600-602 |
, |
denotes |
, |
T2770 |
602-604 |
IN |
denotes |
as |
T2771 |
605-614 |
VBN |
denotes |
described |
T2772 |
615-620 |
RB |
denotes |
above |
T2773 |
620-622 |
, |
denotes |
, |
T2774 |
622-626 |
VBD |
denotes |
were |
T2775 |
637-639 |
IN |
denotes |
at |
T2776 |
640-642 |
CD |
denotes |
30 |
T2777 |
642-644 |
NN |
denotes |
°C |
T2778 |
645-648 |
IN |
denotes |
for |
T2779 |
649-651 |
CD |
denotes |
15 |
T2780 |
652-655 |
NN |
denotes |
min |
T2781 |
655-657 |
, |
denotes |
, |
T2782 |
657-660 |
CC |
denotes |
and |
T2783 |
661-665 |
RB |
denotes |
then |
T2784 |
666-669 |
DT |
denotes |
the |
T2785 |
670-673 |
NN |
denotes |
ATP |
T2786 |
678-683 |
VBN |
denotes |
added |
T2787 |
674-677 |
VBD |
denotes |
was |
T2788 |
684-686 |
IN |
denotes |
at |
T2789 |
687-689 |
CD |
denotes |
10 |
T2790 |
690-692 |
NN |
denotes |
mM |
T2791 |
692-694 |
, |
denotes |
, |
T2792 |
694-702 |
VBN |
denotes |
followed |
T2793 |
703-705 |
IN |
denotes |
by |
T2794 |
706-716 |
NN |
denotes |
incubation |
T2795 |
717-719 |
IN |
denotes |
at |
T2796 |
720-722 |
CD |
denotes |
37 |
T2797 |
722-724 |
NN |
denotes |
°C |
T2798 |
725-728 |
IN |
denotes |
for |
T2799 |
729-739 |
JJ |
denotes |
additional |
T2800 |
743-746 |
NN |
denotes |
min |
T2801 |
740-742 |
CD |
denotes |
30 |
T2802 |
746-747 |
. |
denotes |
. |
T2803 |
747-882 |
sentence |
denotes |
The reactions were terminated by addition of EDTA (20 mM), SDS (0.1%) and 2 μg proteinase K, and were incubated for additional 15 min. |
T2804 |
748-751 |
DT |
denotes |
The |
T2805 |
752-761 |
NNS |
denotes |
reactions |
T2806 |
767-777 |
VBN |
denotes |
terminated |
T2807 |
762-766 |
VBD |
denotes |
were |
T2808 |
778-780 |
IN |
denotes |
by |
T2809 |
781-789 |
NN |
denotes |
addition |
T2810 |
790-792 |
IN |
denotes |
of |
T2811 |
793-797 |
NN |
denotes |
EDTA |
T2812 |
798-799 |
-LRB- |
denotes |
( |
T2813 |
802-804 |
NN |
denotes |
mM |
T2814 |
799-801 |
CD |
denotes |
20 |
T2815 |
804-805 |
-RRB- |
denotes |
) |
T2816 |
805-807 |
, |
denotes |
, |
T2817 |
807-810 |
NN |
denotes |
SDS |
T2818 |
811-812 |
-LRB- |
denotes |
( |
T2819 |
815-816 |
NN |
denotes |
% |
T2820 |
812-815 |
CD |
denotes |
0.1 |
T2821 |
816-817 |
-RRB- |
denotes |
) |
T2822 |
818-821 |
CC |
denotes |
and |
T2823 |
822-823 |
CD |
denotes |
2 |
T2824 |
824-826 |
NN |
denotes |
μg |
T2825 |
838-839 |
NN |
denotes |
K |
T2826 |
827-837 |
NN |
denotes |
proteinase |
T2827 |
839-841 |
, |
denotes |
, |
T2828 |
841-844 |
CC |
denotes |
and |
T2829 |
845-849 |
VBD |
denotes |
were |
T2830 |
850-859 |
VBN |
denotes |
incubated |
T2831 |
860-863 |
IN |
denotes |
for |
T2832 |
864-874 |
JJ |
denotes |
additional |
T2833 |
878-881 |
NN |
denotes |
min |
T2834 |
875-877 |
CD |
denotes |
15 |
T2835 |
881-882 |
. |
denotes |
. |
T2836 |
882-975 |
sentence |
denotes |
The samples were separated by electrophoresis on nondenaturing polyacrylamide gel in 1× TBE. |
T2837 |
883-886 |
DT |
denotes |
The |
T2838 |
887-894 |
NNS |
denotes |
samples |
T2839 |
900-909 |
VBN |
denotes |
separated |
T2840 |
895-899 |
VBD |
denotes |
were |
T2841 |
910-912 |
IN |
denotes |
by |
T2842 |
913-928 |
NN |
denotes |
electrophoresis |
T2843 |
929-931 |
IN |
denotes |
on |
T2844 |
932-945 |
JJ |
denotes |
nondenaturing |
T2845 |
961-964 |
NN |
denotes |
gel |
T2846 |
946-960 |
NN |
denotes |
polyacrylamide |
T2847 |
965-967 |
IN |
denotes |
in |
T2848 |
968-969 |
CD |
denotes |
1 |
T2849 |
971-974 |
NN |
denotes |
TBE |
T2850 |
969-970 |
SYM |
denotes |
× |
T2851 |
974-975 |
. |
denotes |
. |
R1668 |
T2629 |
T2630 |
nmod |
DNA,helicase |
R1669 |
T2630 |
T2631 |
nmod |
helicase,assays |
R1670 |
T2632 |
T2630 |
cc |
and,helicase |
R1671 |
T2633 |
T2634 |
compound |
gel,shift |
R1672 |
T2634 |
T2630 |
conj |
shift,helicase |
R1673 |
T2636 |
T2637 |
prep |
In,incubated |
R1674 |
T2638 |
T2639 |
compound |
gel,shift |
R1675 |
T2639 |
T2640 |
compound |
shift,assays |
R1676 |
T2640 |
T2636 |
pobj |
assays,In |
R1677 |
T2641 |
T2637 |
punct |
", ",incubated |
R1678 |
T2642 |
T2643 |
nmod |
Mcm4,proteins |
R1679 |
T2643 |
T2637 |
nsubjpass |
proteins,incubated |
R1680 |
T2644 |
T2642 |
punct |
/,Mcm4 |
R1681 |
T2645 |
T2642 |
nummod |
6,Mcm4 |
R1682 |
T2646 |
T2642 |
punct |
/,Mcm4 |
R1683 |
T2647 |
T2642 |
nummod |
7,Mcm4 |
R1684 |
T2648 |
T2637 |
auxpass |
were,incubated |
R1685 |
T2649 |
T2637 |
prep |
at,incubated |
R1686 |
T2650 |
T2651 |
nummod |
30,°C |
R1687 |
T2651 |
T2649 |
pobj |
°C,at |
R1688 |
T2652 |
T2637 |
prep |
for,incubated |
R1689 |
T2653 |
T2654 |
nummod |
30,min |
R1690 |
T2654 |
T2652 |
pobj |
min,for |
R1691 |
T2655 |
T2637 |
prep |
in,incubated |
R1692 |
T2656 |
T2657 |
compound |
reaction,mixtures |
R1693 |
T2657 |
T2655 |
pobj |
mixtures,in |
R1694 |
T2658 |
T2659 |
punct |
(,μl |
R1695 |
T2659 |
T2657 |
parataxis |
μl,mixtures |
R1696 |
T2660 |
T2659 |
nummod |
15,μl |
R1697 |
T2661 |
T2659 |
punct |
),μl |
R1698 |
T2662 |
T2657 |
acl |
containing,mixtures |
R1699 |
T2663 |
T2664 |
nummod |
25,mM |
R1700 |
T2664 |
T2665 |
compound |
mM,NaOH |
R1701 |
T2665 |
T2662 |
dobj |
NaOH,containing |
R1702 |
T2666 |
T2665 |
compound |
HEPES,NaOH |
R1703 |
T2667 |
T2665 |
punct |
–,NaOH |
R1704 |
T2668 |
T2669 |
punct |
(,pH |
R1705 |
T2669 |
T2665 |
parataxis |
pH,NaOH |
R1706 |
T2670 |
T2669 |
nummod |
7.5,pH |
R1707 |
T2671 |
T2669 |
punct |
),pH |
R1708 |
T2672 |
T2665 |
punct |
", ",NaOH |
R1709 |
T2673 |
T2674 |
nummod |
50,mM |
R1710 |
T2674 |
T2675 |
compound |
mM,acetate |
R1711 |
T2675 |
T2665 |
conj |
acetate,NaOH |
R1712 |
T2676 |
T2675 |
compound |
sodium,acetate |
R1713 |
T2677 |
T2675 |
punct |
", ",acetate |
R1714 |
T2678 |
T2679 |
nummod |
10,mM |
R1715 |
T2679 |
T2680 |
nmod |
mM,CH3COO |
R1716 |
T2680 |
T2675 |
conj |
CH3COO,acetate |
R1717 |
T2681 |
T2680 |
nmod |
Mg,CH3COO |
R1718 |
T2682 |
T2680 |
punct |
(,CH3COO |
R1719 |
T2683 |
T2680 |
punct |
),CH3COO |
R1720 |
T2684 |
T2680 |
nummod |
2,CH3COO |
R1721 |
T2685 |
T2680 |
punct |
", ",CH3COO |
R1722 |
T2686 |
T2687 |
nummod |
1,mM |
R1723 |
T2687 |
T2688 |
compound |
mM,DTT |
R1724 |
T2688 |
T2680 |
conj |
DTT,CH3COO |
R1725 |
T2689 |
T2688 |
punct |
", ",DTT |
R1726 |
T2690 |
T2691 |
nummod |
0.25,μg |
R1727 |
T2691 |
T2692 |
nmod |
μg,BSA |
R1728 |
T2692 |
T2688 |
conj |
BSA,DTT |
R1729 |
T2693 |
T2694 |
punct |
/,ml |
R1730 |
T2694 |
T2691 |
prep |
ml,μg |
R1731 |
T2695 |
T2692 |
punct |
", ",BSA |
R1732 |
T2696 |
T2697 |
nummod |
0.5,mM |
R1733 |
T2697 |
T2698 |
nmod |
mM,S |
R1734 |
T2698 |
T2692 |
conj |
S,BSA |
R1735 |
T2699 |
T2698 |
nmod |
ATP,S |
R1736 |
T2700 |
T2698 |
punct |
-,S |
R1737 |
T2701 |
T2698 |
punct |
γ,S |
R1738 |
T2702 |
T2698 |
punct |
-,S |
R1739 |
T2703 |
T2698 |
cc |
and,S |
R1740 |
T2704 |
T2705 |
amod |
labeled,substrates |
R1741 |
T2705 |
T2698 |
conj |
substrates,S |
R1742 |
T2706 |
T2705 |
prep |
of,substrates |
R1743 |
T2707 |
T2708 |
det |
the,amount |
R1744 |
T2708 |
T2706 |
pobj |
amount,of |
R1745 |
T2709 |
T2708 |
acl |
indicated,amount |
R1746 |
T2710 |
T2637 |
punct |
.,incubated |
R1747 |
T2712 |
T2713 |
prep |
After,applied |
R1748 |
T2714 |
T2712 |
pobj |
addition,After |
R1749 |
T2715 |
T2714 |
prep |
of,addition |
R1750 |
T2716 |
T2717 |
nummod |
2,μl |
R1751 |
T2717 |
T2715 |
pobj |
μl,of |
R1752 |
T2718 |
T2717 |
prep |
of,μl |
R1753 |
T2719 |
T2720 |
nummod |
50,% |
R1754 |
T2720 |
T2721 |
compound |
%,glycerol |
R1755 |
T2721 |
T2718 |
pobj |
glycerol,of |
R1756 |
T2722 |
T2713 |
punct |
", ",applied |
R1757 |
T2723 |
T2724 |
det |
the,mixtures |
R1758 |
T2724 |
T2713 |
nsubjpass |
mixtures,applied |
R1759 |
T2725 |
T2724 |
compound |
reaction,mixtures |
R1760 |
T2726 |
T2713 |
auxpass |
were,applied |
R1761 |
T2727 |
T2713 |
advmod |
directly,applied |
R1762 |
T2728 |
T2713 |
prep |
on,applied |
R1763 |
T2729 |
T2730 |
det |
a,gel |
R1764 |
T2730 |
T2728 |
pobj |
gel,on |
R1765 |
T2731 |
T2730 |
compound |
polyacrylamide,gel |
R1766 |
T2732 |
T2730 |
acl |
containing,gel |
R1767 |
T2733 |
T2734 |
nummod |
6,mM |
R1768 |
T2734 |
T2735 |
compound |
mM,acetate |
R1769 |
T2735 |
T2732 |
dobj |
acetate,containing |
R1770 |
T2736 |
T2735 |
compound |
magnesium,acetate |
R1771 |
T2737 |
T2735 |
cc |
and,acetate |
R1772 |
T2738 |
T2739 |
nummod |
5,% |
R1773 |
T2739 |
T2740 |
compound |
%,glycerol |
R1774 |
T2740 |
T2735 |
conj |
glycerol,acetate |
R1775 |
T2741 |
T2732 |
prep |
in,containing |
R1776 |
T2742 |
T2743 |
nummod |
0.5,TBE |
R1777 |
T2743 |
T2741 |
pobj |
TBE,in |
R1778 |
T2744 |
T2742 |
punct |
×,0.5 |
R1779 |
T2745 |
T2713 |
punct |
", ",applied |
R1780 |
T2746 |
T2713 |
cc |
and,applied |
R1781 |
T2747 |
T2748 |
nsubjpass |
electrophoresis,conducted |
R1782 |
T2748 |
T2713 |
conj |
conducted,applied |
R1783 |
T2749 |
T2748 |
auxpass |
was,conducted |
R1784 |
T2750 |
T2748 |
prep |
at,conducted |
R1785 |
T2751 |
T2752 |
compound |
room,temperature |
R1786 |
T2752 |
T2750 |
pobj |
temperature,at |
R1787 |
T2753 |
T2748 |
punct |
.,conducted |
R1788 |
T2755 |
T2756 |
prep |
For,incubated |
R1789 |
T2757 |
T2758 |
compound |
DNA,assays |
R1790 |
T2758 |
T2755 |
pobj |
assays,For |
R1791 |
T2759 |
T2758 |
compound |
helicase,assays |
R1792 |
T2760 |
T2756 |
punct |
", ",incubated |
R1793 |
T2761 |
T2762 |
det |
the,mixtures |
R1794 |
T2762 |
T2756 |
nsubjpass |
mixtures,incubated |
R1795 |
T2763 |
T2762 |
compound |
reaction,mixtures |
R1796 |
T2764 |
T2762 |
prep |
of,mixtures |
R1797 |
T2765 |
T2766 |
det |
the,assays |
R1798 |
T2766 |
T2764 |
pobj |
assays,of |
R1799 |
T2767 |
T2768 |
compound |
gel,shift |
R1800 |
T2768 |
T2766 |
compound |
shift,assays |
R1801 |
T2769 |
T2756 |
punct |
", ",incubated |
R1802 |
T2770 |
T2771 |
mark |
as,described |
R1803 |
T2771 |
T2756 |
advcl |
described,incubated |
R1804 |
T2772 |
T2771 |
advmod |
above,described |
R1805 |
T2773 |
T2756 |
punct |
", ",incubated |
R1806 |
T2774 |
T2756 |
auxpass |
were,incubated |
R1807 |
T2775 |
T2756 |
prep |
at,incubated |
R1808 |
T2776 |
T2777 |
nummod |
30,°C |
R1809 |
T2777 |
T2775 |
pobj |
°C,at |
R1810 |
T2778 |
T2756 |
prep |
for,incubated |
R1811 |
T2779 |
T2780 |
nummod |
15,min |
R1812 |
T2780 |
T2778 |
pobj |
min,for |
R1813 |
T2781 |
T2756 |
punct |
", ",incubated |
R1814 |
T2782 |
T2756 |
cc |
and,incubated |
R1815 |
T2783 |
T2782 |
advmod |
then,and |
R1816 |
T2784 |
T2785 |
det |
the,ATP |
R1817 |
T2785 |
T2786 |
nsubjpass |
ATP,added |
R1818 |
T2786 |
T2756 |
conj |
added,incubated |
R1819 |
T2787 |
T2786 |
auxpass |
was,added |
R1820 |
T2788 |
T2786 |
prep |
at,added |
R1821 |
T2789 |
T2790 |
nummod |
10,mM |
R1822 |
T2790 |
T2788 |
pobj |
mM,at |
R1823 |
T2791 |
T2786 |
punct |
", ",added |
R1824 |
T2792 |
T2786 |
advcl |
followed,added |
R1825 |
T2793 |
T2792 |
agent |
by,followed |
R1826 |
T2794 |
T2793 |
pobj |
incubation,by |
R1827 |
T2795 |
T2794 |
prep |
at,incubation |
R1828 |
T2796 |
T2797 |
nummod |
37,°C |
R1829 |
T2797 |
T2795 |
pobj |
°C,at |
R1830 |
T2798 |
T2794 |
prep |
for,incubation |
R1831 |
T2799 |
T2800 |
amod |
additional,min |
R1832 |
T2800 |
T2798 |
pobj |
min,for |
R1833 |
T2801 |
T2800 |
nummod |
30,min |
R1834 |
T2802 |
T2786 |
punct |
.,added |
R1835 |
T2804 |
T2805 |
det |
The,reactions |
R1836 |
T2805 |
T2806 |
nsubjpass |
reactions,terminated |
R1837 |
T2807 |
T2806 |
auxpass |
were,terminated |
R1838 |
T2808 |
T2806 |
agent |
by,terminated |
R1839 |
T2809 |
T2808 |
pobj |
addition,by |
R1840 |
T2810 |
T2809 |
prep |
of,addition |
R1841 |
T2811 |
T2810 |
pobj |
EDTA,of |
R1842 |
T2812 |
T2813 |
punct |
(,mM |
R1843 |
T2813 |
T2811 |
parataxis |
mM,EDTA |
R1844 |
T2814 |
T2813 |
nummod |
20,mM |
R1845 |
T2815 |
T2813 |
punct |
),mM |
R1846 |
T2816 |
T2811 |
punct |
", ",EDTA |
R1847 |
T2817 |
T2811 |
conj |
SDS,EDTA |
R1848 |
T2818 |
T2819 |
punct |
(,% |
R1849 |
T2819 |
T2817 |
parataxis |
%,SDS |
R1850 |
T2820 |
T2819 |
nummod |
0.1,% |
R1851 |
T2821 |
T2819 |
punct |
),% |
R1852 |
T2822 |
T2817 |
cc |
and,SDS |
R1853 |
T2823 |
T2824 |
nummod |
2,μg |
R1854 |
T2824 |
T2825 |
compound |
μg,K |
R1855 |
T2825 |
T2817 |
conj |
K,SDS |
R1856 |
T2826 |
T2825 |
compound |
proteinase,K |
R1857 |
T2827 |
T2806 |
punct |
", ",terminated |
R1858 |
T2828 |
T2806 |
cc |
and,terminated |
R1859 |
T2829 |
T2830 |
auxpass |
were,incubated |
R1860 |
T2830 |
T2806 |
conj |
incubated,terminated |
R1861 |
T2831 |
T2830 |
prep |
for,incubated |
R1862 |
T2832 |
T2833 |
amod |
additional,min |
R1863 |
T2833 |
T2831 |
pobj |
min,for |
R1864 |
T2834 |
T2833 |
nummod |
15,min |
R1865 |
T2835 |
T2806 |
punct |
.,terminated |
R1866 |
T2837 |
T2838 |
det |
The,samples |
R1867 |
T2838 |
T2839 |
nsubjpass |
samples,separated |
R1868 |
T2840 |
T2839 |
auxpass |
were,separated |
R1869 |
T2841 |
T2839 |
prep |
by,separated |
R1870 |
T2842 |
T2841 |
pobj |
electrophoresis,by |
R1871 |
T2843 |
T2839 |
prep |
on,separated |
R1872 |
T2844 |
T2845 |
amod |
nondenaturing,gel |
R1873 |
T2845 |
T2843 |
pobj |
gel,on |
R1874 |
T2846 |
T2845 |
compound |
polyacrylamide,gel |
R1875 |
T2847 |
T2845 |
prep |
in,gel |
R1876 |
T2848 |
T2849 |
nummod |
1,TBE |
R1877 |
T2849 |
T2847 |
pobj |
TBE,in |
R1878 |
T2850 |
T2848 |
punct |
×,1 |
R1879 |
T2851 |
T2839 |
punct |
.,separated |