PMC:1140370 / 19201-20615 JSONTXT

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We found that dT-tailed 3′-extension was displaced efficiently by Mcm 4/6/7 while almost no or very little activity was detected on other substrates (Figure 5B and C). This is in sharp contrast to yeast Mcm4/6/7 helicases which are not capable of unwinding 3′-extension substrates. Furthermore, dT-tailed 5′-extension, to which Mcm4/6/7 could bind, was not displaced by the Mcm helicase. The addition of the 5′-tail to dT-tailed 3′-extension further stimulated the displacement (Figure 5D), confirming the previous report that the fork structures facilitate the helicase action. 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