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contrast, only the substrate containing a 3′ single-stranded DNA tail (A-fork[5′]) was efficiently unwound and A-fork[3′] or A-fork[3′,5′], was not unwound by Mcm4/6/7 (Figure 4B). Absence of helicase actions on A-fork[3′,5′] composed only of duplex regions is expected since double-stranded DNA is not able to activate the ATPase activity of Mcm4/6/7 (10). In contrast, the PriA, a structure-specific helicase used as a control, binds at the fork junction and unwinds the fork and the nascent lagging strand DNA on A-fork[5′], or unwinds the nascent lagging strand on A-fork[3′,5′], as reported before (25). These results indicate that the Mcm4/6/7 complex binds to single-stranded DNA and that its helicase is activated only in the presence of a single-stranded 3′-tail."}
craft-ca-core-ex-dev
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craft-ca-core-dev
{"project":"craft-ca-core-dev","denotations":[{"id":"T5170","span":{"begin":48,"end":63},"obj":"SO:0000984"},{"id":"T5171","span":{"begin":76,"end":80},"obj":"GO:0005657"},{"id":"T5172","span":{"begin":116,"end":120},"obj":"GO:0005657"},{"id":"T5173","span":{"begin":130,"end":134},"obj":"GO:0005657"},{"id":"T5174","span":{"begin":162,"end":166},"obj":"PR:000010246"},{"id":"T5175","span":{"begin":162,"end":170},"obj":"GO:0097373"},{"id":"T5176","span":{"begin":217,"end":221},"obj":"GO:0005657"},{"id":"T5177","span":{"begin":246,"end":252},"obj":"SO:0000985"},{"id":"T5178","span":{"begin":279,"end":294},"obj":"SO:0000985"},{"id":"T5179","span":{"begin":346,"end":350},"obj":"PR:000010246"},{"id":"T5180","span":{"begin":346,"end":354},"obj":"GO:0097373"},{"id":"T5181","span":{"begin":378,"end":382},"obj":"PR:000023591"},{"id":"T5182","span":{"begin":446,"end":450},"obj":"GO:0005657"},{"id":"T5183","span":{"begin":476,"end":480},"obj":"GO:0005657"},{"id":"T5184","span":{"begin":521,"end":525},"obj":"GO:0005657"},{"id":"T5185","span":{"begin":574,"end":578},"obj":"GO:0005657"},{"id":"T5186","span":{"begin":644,"end":648},"obj":"PR:000010246"},{"id":"T5187","span":{"begin":644,"end":660},"obj":"GO:0097373"},{"id":"T5188","span":{"begin":670,"end":685},"obj":"SO:0000984"},{"id":"T5189","span":{"begin":751,"end":766},"obj":"SO:0000984"}],"text":"In contrast, only the substrate containing a 3′ single-stranded DNA tail (A-fork[5′]) was efficiently unwound and A-fork[3′] or A-fork[3′,5′], was not unwound by Mcm4/6/7 (Figure 4B). Absence of helicase actions on A-fork[3′,5′] composed only of duplex regions is expected since double-stranded DNA is not able to activate the ATPase activity of Mcm4/6/7 (10). In contrast, the PriA, a structure-specific helicase used as a control, binds at the fork junction and unwinds the fork and the nascent lagging strand DNA on A-fork[5′], or unwinds the nascent lagging strand on A-fork[3′,5′], as reported before (25). These results indicate that the Mcm4/6/7 complex binds to single-stranded DNA and that its helicase is activated only in the presence of a single-stranded 3′-tail."}
2_test
{"project":"2_test","denotations":[{"id":"15917436-9305914-76775516","span":{"begin":356,"end":358},"obj":"9305914"},{"id":"15917436-12917421-76775517","span":{"begin":607,"end":609},"obj":"12917421"}],"text":"In contrast, only the substrate containing a 3′ single-stranded DNA tail (A-fork[5′]) was efficiently unwound and A-fork[3′] or A-fork[3′,5′], was not unwound by Mcm4/6/7 (Figure 4B). Absence of helicase actions on A-fork[3′,5′] composed only of duplex regions is expected since double-stranded DNA is not able to activate the ATPase activity of Mcm4/6/7 (10). In contrast, the PriA, a structure-specific helicase used as a control, binds at the fork junction and unwinds the fork and the nascent lagging strand DNA on A-fork[5′], or unwinds the nascent lagging strand on A-fork[3′,5′], as reported before (25). These results indicate that the Mcm4/6/7 complex binds to single-stranded DNA and that its helicase is activated only in the presence of a single-stranded 3′-tail."}