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we analysed the cellular and subcellular distribution of Annexin A7 in the developing mouse brain (Fig. 3). At E13–E16 in transverse sections of the embryonic brain, most of the immunoreactive cells are present in the ventricular germinative zone surrounding the lateral ventricle (Fig. 3A, overview E16; Fig. 3H, overview E13). A closer inspection revealed that Annexin A7 is mainly localized in the cytosol at E13 (Fig. 3B,E). During further development the immature cells have rounded up two days later, but they retain Annexin A7 in the cytosol at E15 (Fig. 3C,F). At E16 we observed the first prominent nuclear staining of Annexin A7 (Fig. 3D,G) in cells of the intermediate zone (Fig. 3I, oval), which contains neurons radially migrating towards the growing neopallium cortex [13,14] and also glial cells forming the white matter of the adult cortex. Cells located marginally in the neopallial cortex also exhibit a nuclear stain. Labelling in the ventricular germinative zone is less prominent and the cytosol is no longer more strongly stained than the nucleus."}

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At E13–E16 in transverse sections of the embryonic brain, most of the immunoreactive cells are present in the ventricular germinative zone surrounding the lateral ventricle (Fig. 3A, overview E16; Fig. 3H, overview E13). A closer inspection revealed that Annexin A7 is mainly localized in the cytosol at E13 (Fig. 3B,E). During further development the immature cells have rounded up two days later, but they retain Annexin A7 in the cytosol at E15 (Fig. 3C,F). At E16 we observed the first prominent nuclear staining of Annexin A7 (Fig. 3D,G) in cells of the intermediate zone (Fig. 3I, oval), which contains neurons radially migrating towards the growing neopallium cortex [13,14] and also glial cells forming the white matter of the adult cortex. Cells located marginally in the neopallial cortex also exhibit a nuclear stain. Labelling in the ventricular germinative zone is less prominent and the cytosol is no longer more strongly stained than the nucleus."}

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