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confirmed the presence of the protein during early development. Annexin A7 immunoreactivity using mAb 203–217, which specifically recognizes both isoforms of Annexin A7, can be observed in the two cell types of the cylinder stage at E5 with a weaker Annexin A7 expression in the ectoderm (Fig. 2A–C). At E8 Annexin A7 can be detected in almost all tissues of the embryo (data not shown). A correlation between the presence of Annexin A7 and the origin of a cell type from one of the three germ layers is not apparent. We specifically noted the localization of the protein in the neuroepithelium of the neural fold and tube (Fig. 2D–F, proximal, E8; Fig. 2G–I, distal, E13). At the subcellular level, Annexin A7 was present in punctate structures mainly in the cytosol of all examined cells (Fig. 2E,F). This distribution was also observed during subsequent development."}

    craft-ca-core-ex-dev

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    biosemtest

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confirmed the presence of the protein during early development. Annexin A7 immunoreactivity using mAb 203–217, which specifically recognizes both isoforms of Annexin A7, can be observed in the two cell types of the cylinder stage at E5 with a weaker Annexin A7 expression in the ectoderm (Fig. 2A–C). At E8 Annexin A7 can be detected in almost all tissues of the embryo (data not shown). A correlation between the presence of Annexin A7 and the origin of a cell type from one of the three germ layers is not apparent. We specifically noted the localization of the protein in the neuroepithelium of the neural fold and tube (Fig. 2D–F, proximal, E8; Fig. 2G–I, distal, E13). At the subcellular level, Annexin A7 was present in punctate structures mainly in the cytosol of all examined cells (Fig. 2E,F). This distribution was also observed during subsequent development."}

    craft-ca-core-dev

    {"project":"craft-ca-core-dev","denotations":[{"id":"T2207","span":{"begin":236,"end":244},"obj":"UBERON:0004013"},{"id":"T2208","span":{"begin":271,"end":281},"obj":"PR:000004080"},{"id":"T2209","span":{"begin":282,"end":292},"obj":"GO:0010467"},{"id":"T2210","span":{"begin":300,"end":308},"obj":"UBERON:0000924"},{"id":"T2211","span":{"begin":328,"end":338},"obj":"PR:000004080"},{"id":"T2212","span":{"begin":369,"end":379},"obj":"_FRAGMENT"},{"id":"T2213","span":{"begin":384,"end":390},"obj":"UBERON:0005291"},{"id":"T2214","span":{"begin":447,"end":457},"obj":"PR:000004080"},{"id":"T2215","span":{"begin":510,"end":521},"obj":"UBERON:0000923"},{"id":"T2216","span":{"begin":600,"end":615},"obj":"UBERON:0034705"},{"id":"T2217","span":{"begin":623,"end":634},"obj":"UBERON:0005062"},{"id":"T2218","span":{"begin":623,"end":629},"obj":"_FRAGMENT"},{"id":"T2219","span":{"begin":639,"end":643},"obj":"UBERON:0001049"},{"id":"T2220","span":{"begin":721,"end":731},"obj":"PR:000004080"},{"id":"T2221","span":{"begin":781,"end":788},"obj":"GO:0005829"},{"id":"T2204","span":{"begin":85,"end":95},"obj":"PR:000004080"},{"id":"T2205","span":{"begin":167,"end":175},"obj":"SO:0001060"},{"id":"T2206","span":{"begin":179,"end":189},"obj":"PR:000004080"}],"relations":[{"id":"R767","pred":"_lexicallyChainedTo","subj":"T2213","obj":"T2212"},{"id":"R768","pred":"_lexicallyChainedTo","subj":"T2219","obj":"T2218"}],"text":"Immunohistochemistry confirmed the presence of the protein during early development. Annexin A7 immunoreactivity using mAb 203–217, which specifically recognizes both isoforms of Annexin A7, can be observed in the two cell types of the cylinder stage at E5 with a weaker Annexin A7 expression in the ectoderm (Fig. 2A–C). At E8 Annexin A7 can be detected in almost all tissues of the embryo (data not shown). A correlation between the presence of Annexin A7 and the origin of a cell type from one of the three germ layers is not apparent. We specifically noted the localization of the protein in the neuroepithelium of the neural fold and tube (Fig. 2D–F, proximal, E8; Fig. 2G–I, distal, E13). At the subcellular level, Annexin A7 was present in punctate structures mainly in the cytosol of all examined cells (Fig. 2E,F). This distribution was also observed during subsequent development."}