PMC:1087847 / 38001-38335
Annnotations
{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/1087847","sourcedb":"PMC","sourceid":"1087847","source_url":"http://www.ncbi.nlm.nih.gov/pmc/1087847","text":"Western blotting\nCells or cellular fractions were lysed in SDS sample buffer and homogenates were subjected to SDS-PAGE. Western blotting was carried out using the method of Towbin et al. [38]. Peroxidase-coupled secondary antibodies (Sigma) and the supersignal enhanced chemiluminescence system (Pierce, Rockford, IL, USA) were used.","divisions":[{"label":"title","span":{"begin":0,"end":16}}],"tracks":[{"project":"craft-sa-dev","denotations":[{"id":"T10896","span":{"begin":0,"end":7},"obj":"NNP"},{"id":"T10897","span":{"begin":8,"end":16},"obj":"NN"},{"id":"T10898","span":{"begin":16,"end":120},"obj":"sentence"},{"id":"T10899","span":{"begin":17,"end":22},"obj":"NNS"},{"id":"T10900","span":{"begin":50,"end":55},"obj":"VBN"},{"id":"T10901","span":{"begin":23,"end":25},"obj":"CC"},{"id":"T10902","span":{"begin":26,"end":34},"obj":"JJ"},{"id":"T10903","span":{"begin":35,"end":44},"obj":"NNS"},{"id":"T10904","span":{"begin":45,"end":49},"obj":"VBD"},{"id":"T10905","span":{"begin":56,"end":58},"obj":"IN"},{"id":"T10906","span":{"begin":59,"end":62},"obj":"NN"},{"id":"T10907","span":{"begin":70,"end":76},"obj":"NN"},{"id":"T10908","span":{"begin":63,"end":69},"obj":"NN"},{"id":"T10909","span":{"begin":77,"end":80},"obj":"CC"},{"id":"T10910","span":{"begin":81,"end":92},"obj":"NNS"},{"id":"T10911","span":{"begin":98,"end":107},"obj":"VBN"},{"id":"T10912","span":{"begin":93,"end":97},"obj":"VBD"},{"id":"T10913","span":{"begin":108,"end":110},"obj":"IN"},{"id":"T10914","span":{"begin":111,"end":114},"obj":"NN"},{"id":"T10915","span":{"begin":115,"end":119},"obj":"NN"},{"id":"T10916","span":{"begin":114,"end":115},"obj":"HYPH"},{"id":"T10917","span":{"begin":119,"end":120},"obj":"."},{"id":"T10918","span":{"begin":120,"end":193},"obj":"sentence"},{"id":"T10919","span":{"begin":121,"end":128},"obj":"NNP"},{"id":"T10920","span":{"begin":129,"end":137},"obj":"NN"},{"id":"T10921","span":{"begin":142,"end":149},"obj":"VBN"},{"id":"T10922","span":{"begin":138,"end":141},"obj":"VBD"},{"id":"T10923","span":{"begin":150,"end":153},"obj":"RP"},{"id":"T10924","span":{"begin":154,"end":159},"obj":"VBG"},{"id":"T10925","span":{"begin":160,"end":163},"obj":"DT"},{"id":"T10926","span":{"begin":164,"end":170},"obj":"NN"},{"id":"T10927","span":{"begin":171,"end":173},"obj":"IN"},{"id":"T10928","span":{"begin":174,"end":180},"obj":"NNP"},{"id":"T10929","span":{"begin":181,"end":183},"obj":"FW"},{"id":"T10930","span":{"begin":184,"end":187},"obj":"FW"},{"id":"T10931","span":{"begin":188,"end":189},"obj":"-LRB-"},{"id":"T10932","span":{"begin":189,"end":191},"obj":"CD"},{"id":"T10933","span":{"begin":191,"end":192},"obj":"-RRB-"},{"id":"T10934","span":{"begin":192,"end":193},"obj":"."},{"id":"T10935","span":{"begin":193,"end":334},"obj":"sentence"},{"id":"T10936","span":{"begin":194,"end":204},"obj":"NN"},{"id":"T10937","span":{"begin":205,"end":212},"obj":"VBN"},{"id":"T10938","span":{"begin":204,"end":205},"obj":"HYPH"},{"id":"T10939","span":{"begin":223,"end":233},"obj":"NNS"},{"id":"T10940","span":{"begin":213,"end":222},"obj":"JJ"},{"id":"T10941","span":{"begin":329,"end":333},"obj":"VBN"},{"id":"T10942","span":{"begin":234,"end":235},"obj":"-LRB-"},{"id":"T10943","span":{"begin":235,"end":240},"obj":"NNP"},{"id":"T10944","span":{"begin":240,"end":241},"obj":"-RRB-"},{"id":"T10945","span":{"begin":242,"end":245},"obj":"CC"},{"id":"T10946","span":{"begin":246,"end":249},"obj":"DT"},{"id":"T10947","span":{"begin":289,"end":295},"obj":"NN"},{"id":"T10948","span":{"begin":250,"end":261},"obj":"NN"},{"id":"T10949","span":{"begin":262,"end":270},"obj":"VBN"},{"id":"T10950","span":{"begin":271,"end":288},"obj":"NN"},{"id":"T10951","span":{"begin":296,"end":297},"obj":"-LRB-"},{"id":"T10952","span":{"begin":297,"end":303},"obj":"NNP"},{"id":"T10953","span":{"begin":303,"end":305},"obj":","},{"id":"T10954","span":{"begin":305,"end":313},"obj":"NNP"},{"id":"T10955","span":{"begin":313,"end":315},"obj":","},{"id":"T10956","span":{"begin":315,"end":317},"obj":"NNP"},{"id":"T10957","span":{"begin":317,"end":319},"obj":","},{"id":"T10958","span":{"begin":319,"end":322},"obj":"NNP"},{"id":"T10959","span":{"begin":322,"end":323},"obj":"-RRB-"},{"id":"T10960","span":{"begin":324,"end":328},"obj":"VBD"},{"id":"T10961","span":{"begin":333,"end":334},"obj":"."}],"relations":[{"id":"R5377","pred":"compound","subj":"T10896","obj":"T10897"},{"id":"R5378","pred":"nsubjpass","subj":"T10899","obj":"T10900"},{"id":"R5379","pred":"cc","subj":"T10901","obj":"T10899"},{"id":"R5380","pred":"amod","subj":"T10902","obj":"T10903"},{"id":"R5381","pred":"conj","subj":"T10903","obj":"T10899"},{"id":"R5382","pred":"auxpass","subj":"T10904","obj":"T10900"},{"id":"R5383","pred":"prep","subj":"T10905","obj":"T10900"},{"id":"R5384","pred":"compound","subj":"T10906","obj":"T10907"},{"id":"R5385","pred":"pobj","subj":"T10907","obj":"T10905"},{"id":"R5386","pred":"compound","subj":"T10908","obj":"T10907"},{"id":"R5387","pred":"cc","subj":"T10909","obj":"T10900"},{"id":"R5388","pred":"nsubjpass","subj":"T10910","obj":"T10911"},{"id":"R5389","pred":"conj","subj":"T10911","obj":"T10900"},{"id":"R5390","pred":"auxpass","subj":"T10912","obj":"T10911"},{"id":"R5391","pred":"prep","subj":"T10913","obj":"T10911"},{"id":"R5392","pred":"compound","subj":"T10914","obj":"T10915"},{"id":"R5393","pred":"pobj","subj":"T10915","obj":"T10913"},{"id":"R5394","pred":"punct","subj":"T10916","obj":"T10915"},{"id":"R5395","pred":"punct","subj":"T10917","obj":"T10911"},{"id":"R5396","pred":"compound","subj":"T10919","obj":"T10920"},{"id":"R5397","pred":"nsubjpass","subj":"T10920","obj":"T10921"},{"id":"R5398","pred":"auxpass","subj":"T10922","obj":"T10921"},{"id":"R5399","pred":"prt","subj":"T10923","obj":"T10921"},{"id":"R5400","pred":"advcl","subj":"T10924","obj":"T10921"},{"id":"R5401","pred":"det","subj":"T10925","obj":"T10926"},{"id":"R5402","pred":"dobj","subj":"T10926","obj":"T10924"},{"id":"R5403","pred":"prep","subj":"T10927","obj":"T10926"},{"id":"R5404","pred":"pobj","subj":"T10928","obj":"T10927"},{"id":"R5405","pred":"advmod","subj":"T10929","obj":"T10930"},{"id":"R5406","pred":"advmod","subj":"T10930","obj":"T10928"},{"id":"R5407","pred":"punct","subj":"T10931","obj":"T10932"},{"id":"R5408","pred":"parataxis","subj":"T10932","obj":"T10921"},{"id":"R5409","pred":"punct","subj":"T10933","obj":"T10932"},{"id":"R5410","pred":"punct","subj":"T10934","obj":"T10921"},{"id":"R5411","pred":"npadvmod","subj":"T10936","obj":"T10937"},{"id":"R5412","pred":"amod","subj":"T10937","obj":"T10939"},{"id":"R5413","pred":"punct","subj":"T10938","obj":"T10937"},{"id":"R5414","pred":"nsubjpass","subj":"T10939","obj":"T10941"},{"id":"R5415","pred":"amod","subj":"T10940","obj":"T10939"},{"id":"R5416","pred":"punct","subj":"T10942","obj":"T10943"},{"id":"R5417","pred":"parataxis","subj":"T10943","obj":"T10939"},{"id":"R5418","pred":"punct","subj":"T10944","obj":"T10943"},{"id":"R5419","pred":"cc","subj":"T10945","obj":"T10939"},{"id":"R5420","pred":"det","subj":"T10946","obj":"T10947"},{"id":"R5421","pred":"conj","subj":"T10947","obj":"T10939"},{"id":"R5422","pred":"npadvmod","subj":"T10948","obj":"T10949"},{"id":"R5423","pred":"amod","subj":"T10949","obj":"T10947"},{"id":"R5424","pred":"compound","subj":"T10950","obj":"T10947"},{"id":"R5425","pred":"punct","subj":"T10951","obj":"T10952"},{"id":"R5426","pred":"parataxis","subj":"T10952","obj":"T10947"},{"id":"R5427","pred":"punct","subj":"T10953","obj":"T10952"},{"id":"R5428","pred":"npadvmod","subj":"T10954","obj":"T10952"},{"id":"R5429","pred":"punct","subj":"T10955","obj":"T10952"},{"id":"R5430","pred":"npadvmod","subj":"T10956","obj":"T10952"},{"id":"R5431","pred":"punct","subj":"T10957","obj":"T10952"},{"id":"R5432","pred":"npadvmod","subj":"T10958","obj":"T10952"},{"id":"R5433","pred":"punct","subj":"T10959","obj":"T10952"},{"id":"R5434","pred":"auxpass","subj":"T10960","obj":"T10941"},{"id":"R5435","pred":"punct","subj":"T10961","obj":"T10941"}],"attributes":[{"subj":"T10896","pred":"source","obj":"craft-sa-dev"},{"subj":"T10897","pred":"source","obj":"craft-sa-dev"},{"subj":"T10898","pred":"source","obj":"craft-sa-dev"},{"subj":"T10899","pred":"source","obj":"craft-sa-dev"},{"subj":"T10900","pred":"source","obj":"craft-sa-dev"},{"subj":"T10901","pred":"source","obj":"craft-sa-dev"},{"subj":"T10902","pred":"source","obj":"craft-sa-dev"},{"subj":"T10903","pred":"source","obj":"craft-sa-dev"},{"subj":"T10904","pred":"source","obj":"craft-sa-dev"},{"subj":"T10905","pred":"source","obj":"craft-sa-dev"},{"subj":"T10906","pred":"source","obj":"craft-sa-dev"},{"subj":"T10907","pred":"source","obj":"craft-sa-dev"},{"subj":"T10908","pred":"source","obj":"craft-sa-dev"},{"subj":"T10909","pred":"source","obj":"craft-sa-dev"},{"subj":"T10910","pred":"source","obj":"craft-sa-dev"},{"subj":"T10911","pred":"source","obj":"craft-sa-dev"},{"subj":"T10912","pred":"source","obj":"craft-sa-dev"},{"subj":"T10913","pred":"source","obj":"craft-sa-dev"},{"subj":"T10914","pred":"source","obj":"craft-sa-dev"},{"subj":"T10915","pred":"source","obj":"craft-sa-dev"},{"subj":"T10916","pred":"source","obj":"craft-sa-dev"},{"subj":"T10917","pred":"source","obj":"craft-sa-dev"},{"subj":"T10918","pred":"source","obj":"craft-sa-dev"},{"subj":"T10919","pred":"source","obj":"craft-sa-dev"},{"subj":"T10920","pred":"source","obj":"craft-sa-dev"},{"subj":"T10921","pred":"source","obj":"craft-sa-dev"},{"subj":"T10922","pred":"source","obj":"craft-sa-dev"},{"subj":"T10923","pred":"source","obj":"craft-sa-dev"},{"subj":"T10924","pred":"source","obj":"craft-sa-dev"},{"subj":"T10925","pred":"source","obj":"craft-sa-dev"},{"subj":"T10926","pred":"source","obj":"craft-sa-dev"},{"subj":"T10927","pred":"source","obj":"craft-sa-dev"},{"subj":"T10928","pred":"source","obj":"craft-sa-dev"},{"subj":"T10929","pred":"source","obj":"craft-sa-dev"},{"subj":"T10930","pred":"source","obj":"craft-sa-dev"},{"subj":"T10931","pred":"source","obj":"craft-sa-dev"},{"subj":"T10932","pred":"source","obj":"craft-sa-dev"},{"subj":"T10933","pred":"source","obj":"craft-sa-dev"},{"subj":"T10934","pred":"source","obj":"craft-sa-dev"},{"subj":"T10935","pred":"source","obj":"craft-sa-dev"},{"subj":"T10936","pred":"source","obj":"craft-sa-dev"},{"subj":"T10937","pred":"source","obj":"craft-sa-dev"},{"subj":"T10938","pred":"source","obj":"craft-sa-dev"},{"subj":"T10939","pred":"source","obj":"craft-sa-dev"},{"subj":"T10940","pred":"source","obj":"craft-sa-dev"},{"subj":"T10941","pred":"source","obj":"craft-sa-dev"},{"subj":"T10942","pred":"source","obj":"craft-sa-dev"},{"subj":"T10943","pred":"source","obj":"craft-sa-dev"},{"subj":"T10944","pred":"source","obj":"craft-sa-dev"},{"subj":"T10945","pred":"source","obj":"craft-sa-dev"},{"subj":"T10946","pred":"source","obj":"craft-sa-dev"},{"subj":"T10947","pred":"source","obj":"craft-sa-dev"},{"subj":"T10948","pred":"source","obj":"craft-sa-dev"},{"subj":"T10949","pred":"source","obj":"craft-sa-dev"},{"subj":"T10950","pred":"source","obj":"craft-sa-dev"},{"subj":"T10951","pred":"source","obj":"craft-sa-dev"},{"subj":"T10952","pred":"source","obj":"craft-sa-dev"},{"subj":"T10953","pred":"source","obj":"craft-sa-dev"},{"subj":"T10954","pred":"source","obj":"craft-sa-dev"},{"subj":"T10955","pred":"source","obj":"craft-sa-dev"},{"subj":"T10956","pred":"source","obj":"craft-sa-dev"},{"subj":"T10957","pred":"source","obj":"craft-sa-dev"},{"subj":"T10958","pred":"source","obj":"craft-sa-dev"},{"subj":"T10959","pred":"source","obj":"craft-sa-dev"},{"subj":"T10960","pred":"source","obj":"craft-sa-dev"},{"subj":"T10961","pred":"source","obj":"craft-sa-dev"}]},{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T10887","span":{"begin":17,"end":22},"obj":"CL_GO_EXT:cell"},{"id":"T10888","span":{"begin":26,"end":34},"obj":"CL_GO_EXT:cell"},{"id":"T10889","span":{"begin":50,"end":55},"obj":"GO:0019835"},{"id":"T10890","span":{"begin":59,"end":62},"obj":"CHEBI:8984"},{"id":"T10891","span":{"begin":70,"end":76},"obj":"CHEBI_CHMO_EXT:buffer_solution"},{"id":"T10892","span":{"begin":111,"end":114},"obj":"CHEBI:8984"},{"id":"T10893","span":{"begin":194,"end":204},"obj":"GO_EXT:0004601"},{"id":"T10894","span":{"begin":205,"end":212},"obj":"MOP:0000779"},{"id":"T10895","span":{"begin":223,"end":233},"obj":"GO:0042571"}],"attributes":[{"subj":"T10887","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T10888","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T10889","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T10890","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T10891","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T10892","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T10893","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T10894","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T10895","pred":"source","obj":"craft-ca-core-ex-dev"}]},{"project":"craft-ca-core-dev","denotations":[{"id":"T10882","span":{"begin":50,"end":55},"obj":"GO:0019835"},{"id":"T10883","span":{"begin":59,"end":62},"obj":"CHEBI:8984"},{"id":"T10884","span":{"begin":111,"end":114},"obj":"CHEBI:8984"},{"id":"T10885","span":{"begin":205,"end":212},"obj":"MOP:0000779"},{"id":"T10886","span":{"begin":223,"end":233},"obj":"GO:0042571"}],"attributes":[{"subj":"T10882","pred":"source","obj":"craft-ca-core-dev"},{"subj":"T10883","pred":"source","obj":"craft-ca-core-dev"},{"subj":"T10884","pred":"source","obj":"craft-ca-core-dev"},{"subj":"T10885","pred":"source","obj":"craft-ca-core-dev"},{"subj":"T10886","pred":"source","obj":"craft-ca-core-dev"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"craft-sa-dev","color":"#a193ec","default":true},{"id":"craft-ca-core-ex-dev","color":"#9eec93"},{"id":"craft-ca-core-dev","color":"#ec93b8"}]}]}}