PMC:1087847 / 37187-37457
Annnotations
craft-sa-dev
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For partial extraction of the nucleoplasm according to [37], nuclei were resuspended in buffer 2 (20 mM Hepes, pH 7.9, 1.5 mM MgCl2, 20 mM KCl, 0.2 mM EDTA, 25%v/v glycerine, 0.5 mM DTT, 0.5 mM PMSF; nuclei of 107cells/30 μl buffer) and incubated on ice for 30 minutes."}
craft-ca-core-ex-dev
{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T10510","span":{"begin":31,"end":42},"obj":"GO:0005654"},{"id":"T10511","span":{"begin":62,"end":68},"obj":"GO:0005634"},{"id":"T10512","span":{"begin":89,"end":95},"obj":"CHEBI_CHMO_EXT:buffer_solution"},{"id":"T10513","span":{"begin":105,"end":110},"obj":"CHEBI:46756"},{"id":"T10514","span":{"begin":127,"end":132},"obj":"CHEBI:6636"},{"id":"T10515","span":{"begin":140,"end":143},"obj":"CHEBI:32588"},{"id":"T10516","span":{"begin":152,"end":156},"obj":"CHEBI_EXT:EDTA"},{"id":"T10517","span":{"begin":165,"end":174},"obj":"CHEBI:17754"},{"id":"T10518","span":{"begin":183,"end":186},"obj":"CHEBI:18320"},{"id":"T10519","span":{"begin":195,"end":199},"obj":"CHEBI:8102"},{"id":"T10520","span":{"begin":201,"end":207},"obj":"GO:0005634"},{"id":"T10521","span":{"begin":226,"end":232},"obj":"CHEBI_CHMO_EXT:buffer_solution"}],"text":" For partial extraction of the nucleoplasm according to [37], nuclei were resuspended in buffer 2 (20 mM Hepes, pH 7.9, 1.5 mM MgCl2, 20 mM KCl, 0.2 mM EDTA, 25%v/v glycerine, 0.5 mM DTT, 0.5 mM PMSF; nuclei of 107cells/30 μl buffer) and incubated on ice for 30 minutes."}
biosemtest
{"project":"biosemtest","denotations":[{"id":"T10271","span":{"begin":114,"end":116},"obj":"UMLS/C0020283"},{"id":"T10279","span":{"begin":55,"end":57},"obj":"UMLS/C0319022"},{"id":"T10286","span":{"begin":254,"end":257},"obj":"UMLS/C0025611"},{"id":"T10298","span":{"begin":185,"end":188},"obj":"UMLS/C0012789"},{"id":"T10304","span":{"begin":241,"end":250},"obj":"UMLS/C1439852"},{"id":"T10314","span":{"begin":154,"end":158},"obj":"UMLS/C0013618"},{"id":"T10338","span":{"begin":91,"end":97},"obj":"UMLS/C0006353"},{"id":"T10339","span":{"begin":229,"end":235},"obj":"UMLS/C0006353"},{"id":"T10351","span":{"begin":7,"end":14},"obj":"UMLS/C0728938"},{"id":"T10352","span":{"begin":33,"end":44},"obj":"UMLS/C0682537"},{"id":"T10355","span":{"begin":45,"end":54},"obj":"UMLS/C0680240"},{"id":"T10362","span":{"begin":114,"end":116},"obj":"UMLS/C0450407"},{"id":"T10366","span":{"begin":114,"end":116},"obj":"UMLS/C0450408"},{"id":"T10369","span":{"begin":114,"end":116},"obj":"UMLS/C0450409"},{"id":"T10373","span":{"begin":114,"end":116},"obj":"UMLS/C2363150"},{"id":"T10377","span":{"begin":114,"end":116},"obj":"UMLS/C0031526"},{"id":"T10391","span":{"begin":15,"end":25},"obj":"UMLS/C0684295"},{"id":"T10401","span":{"begin":254,"end":257},"obj":"UMLS/C0349714"},{"id":"T10421","span":{"begin":15,"end":25},"obj":"UMLS/C0185115"},{"id":"T10422","span":{"begin":167,"end":176},"obj":"UMLS/C0017861"},{"id":"T10425","span":{"begin":154,"end":158},"obj":"UMLS/C0558745"},{"id":"T10428","span":{"begin":7,"end":14},"obj":"UMLS/C1550516"},{"id":"T10431","span":{"begin":129,"end":134},"obj":"UMLS/C0024472"},{"id":"T10437","span":{"begin":114,"end":116},"obj":"UMLS/C1304686"},{"id":"T10446","span":{"begin":254,"end":257},"obj":"UMLS/C0020746"},{"id":"T10455","span":{"begin":64,"end":70},"obj":"UMLS/C0007610"},{"id":"T10456","span":{"begin":203,"end":209},"obj":"UMLS/C0007610"}],"text":" For partial extraction of the nucleoplasm according to [37], nuclei were resuspended in buffer 2 (20 mM Hepes, pH 7.9, 1.5 mM MgCl2, 20 mM KCl, 0.2 mM EDTA, 25%v/v glycerine, 0.5 mM DTT, 0.5 mM PMSF; nuclei of 107cells/30 μl buffer) and incubated on ice for 30 minutes."}
craft-ca-core-dev
{"project":"craft-ca-core-dev","denotations":[{"id":"T10469","span":{"begin":31,"end":42},"obj":"GO:0005654"},{"id":"T10470","span":{"begin":62,"end":68},"obj":"GO:0005634"},{"id":"T10471","span":{"begin":105,"end":110},"obj":"CHEBI:46756"},{"id":"T10472","span":{"begin":127,"end":132},"obj":"CHEBI:6636"},{"id":"T10473","span":{"begin":140,"end":143},"obj":"CHEBI:32588"},{"id":"T10474","span":{"begin":165,"end":174},"obj":"CHEBI:17754"},{"id":"T10475","span":{"begin":183,"end":186},"obj":"CHEBI:18320"},{"id":"T10476","span":{"begin":195,"end":199},"obj":"CHEBI:8102"},{"id":"T10477","span":{"begin":201,"end":207},"obj":"GO:0005634"}],"text":" For partial extraction of the nucleoplasm according to [37], nuclei were resuspended in buffer 2 (20 mM Hepes, pH 7.9, 1.5 mM MgCl2, 20 mM KCl, 0.2 mM EDTA, 25%v/v glycerine, 0.5 mM DTT, 0.5 mM PMSF; nuclei of 107cells/30 μl buffer) and incubated on ice for 30 minutes."}