Id |
Subject |
Object |
Predicate |
Lexical cue |
T9710 |
0-3 |
DT |
denotes |
The |
T9711 |
22-30 |
NNS |
denotes |
sections |
T9712 |
4-12 |
NN |
denotes |
paraffin |
T9713 |
13-21 |
VBN |
denotes |
embedded |
T9714 |
12-13 |
HYPH |
denotes |
- |
T9715 |
42-49 |
VBN |
denotes |
treated |
T9716 |
31-35 |
VBD |
denotes |
were |
T9717 |
36-41 |
RB |
denotes |
first |
T9718 |
50-55 |
CD |
denotes |
three |
T9719 |
56-61 |
NNS |
denotes |
times |
T9720 |
62-65 |
IN |
denotes |
for |
T9721 |
66-67 |
CD |
denotes |
2 |
T9722 |
68-71 |
NN |
denotes |
min |
T9723 |
72-74 |
IN |
denotes |
in |
T9724 |
75-81 |
NN |
denotes |
xylene |
T9725 |
82-84 |
TO |
denotes |
to |
T9726 |
85-91 |
VB |
denotes |
remove |
T9727 |
92-100 |
NN |
denotes |
paraffin |
T9728 |
101-104 |
CC |
denotes |
and |
T9729 |
105-109 |
RB |
denotes |
then |
T9730 |
110-120 |
VBD |
denotes |
rehydrated |
T9731 |
121-125 |
NN |
denotes |
step |
T9732 |
126-128 |
IN |
denotes |
by |
T9733 |
129-133 |
NN |
denotes |
step |
T9734 |
134-138 |
IN |
denotes |
with |
T9735 |
139-149 |
VBG |
denotes |
descending |
T9736 |
150-164 |
NNS |
denotes |
concentrations |
T9737 |
165-167 |
IN |
denotes |
of |
T9738 |
168-175 |
NN |
denotes |
ethanol |
T9739 |
176-179 |
IN |
denotes |
for |
T9740 |
180-188 |
NN |
denotes |
antibody |
T9741 |
189-197 |
NN |
denotes |
staining |
T9742 |
198-199 |
-LRB- |
denotes |
[ |
T9743 |
199-201 |
CD |
denotes |
35 |
T9744 |
201-202 |
-RRB- |
denotes |
] |
T9745 |
202-203 |
. |
denotes |
. |
T9746 |
203-445 |
sentence |
denotes |
After three washings in PBS (137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.4), the tissue sections were incubated in PBS containing 0.1% trypsin for 10 min at room temperature to free formaldehyde-crosslinked epitopes [15-17]. |
T9747 |
204-209 |
IN |
denotes |
After |
T9748 |
323-332 |
VBN |
denotes |
incubated |
T9749 |
210-215 |
CD |
denotes |
three |
T9750 |
216-224 |
NNS |
denotes |
washings |
T9751 |
225-227 |
IN |
denotes |
in |
T9752 |
228-231 |
NN |
denotes |
PBS |
T9753 |
232-233 |
-LRB- |
denotes |
( |
T9754 |
240-244 |
NN |
denotes |
NaCl |
T9755 |
233-236 |
CD |
denotes |
137 |
T9756 |
237-239 |
NN |
denotes |
mM |
T9757 |
244-246 |
, |
denotes |
, |
T9758 |
246-249 |
CD |
denotes |
2.7 |
T9759 |
250-252 |
NN |
denotes |
mM |
T9760 |
253-256 |
NN |
denotes |
KCl |
T9761 |
256-258 |
, |
denotes |
, |
T9762 |
258-261 |
CD |
denotes |
8.1 |
T9763 |
262-264 |
NN |
denotes |
mM |
T9764 |
265-272 |
NN |
denotes |
Na2HPO4 |
T9765 |
272-274 |
, |
denotes |
, |
T9766 |
274-277 |
CD |
denotes |
1.5 |
T9767 |
278-280 |
NN |
denotes |
mM |
T9768 |
281-287 |
NN |
denotes |
KH2PO4 |
T9769 |
287-289 |
, |
denotes |
, |
T9770 |
289-291 |
NN |
denotes |
pH |
T9771 |
292-295 |
CD |
denotes |
7.4 |
T9772 |
295-296 |
-RRB- |
denotes |
) |
T9773 |
296-298 |
, |
denotes |
, |
T9774 |
298-301 |
DT |
denotes |
the |
T9775 |
309-317 |
NNS |
denotes |
sections |
T9776 |
302-308 |
NN |
denotes |
tissue |
T9777 |
318-322 |
VBD |
denotes |
were |
T9778 |
333-335 |
IN |
denotes |
in |
T9779 |
336-339 |
NN |
denotes |
PBS |
T9780 |
340-350 |
VBG |
denotes |
containing |
T9781 |
351-354 |
CD |
denotes |
0.1 |
T9782 |
354-355 |
NN |
denotes |
% |
T9783 |
356-363 |
NN |
denotes |
trypsin |
T9784 |
364-367 |
IN |
denotes |
for |
T9785 |
368-370 |
CD |
denotes |
10 |
T9786 |
371-374 |
NN |
denotes |
min |
T9787 |
375-377 |
IN |
denotes |
at |
T9788 |
378-382 |
NN |
denotes |
room |
T9789 |
383-394 |
NN |
denotes |
temperature |
T9790 |
395-397 |
TO |
denotes |
to |
T9791 |
398-402 |
VB |
denotes |
free |
T9792 |
403-415 |
NN |
denotes |
formaldehyde |
T9793 |
416-427 |
VBN |
denotes |
crosslinked |
T9794 |
415-416 |
HYPH |
denotes |
- |
T9795 |
428-436 |
NNS |
denotes |
epitopes |
T9796 |
437-438 |
-LRB- |
denotes |
[ |
T9797 |
438-440 |
CD |
denotes |
15 |
T9798 |
440-441 |
SYM |
denotes |
- |
T9799 |
441-443 |
CD |
denotes |
17 |
T9800 |
443-444 |
-RRB- |
denotes |
] |
T9801 |
444-445 |
. |
denotes |
. |
T9802 |
445-641 |
sentence |
denotes |
After additional three washes with PBS, non-specific binding sites were blocked by incubating the slides with PBG (0.5 % BSA, 0.045 % fish gelatine in PBS) in 10% normal goat serum (NGS) for 2 h. |
T9803 |
446-451 |
IN |
denotes |
After |
T9804 |
518-525 |
VBN |
denotes |
blocked |
T9805 |
452-462 |
JJ |
denotes |
additional |
T9806 |
469-475 |
NNS |
denotes |
washes |
T9807 |
463-468 |
CD |
denotes |
three |
T9808 |
476-480 |
IN |
denotes |
with |
T9809 |
481-484 |
NN |
denotes |
PBS |
T9810 |
484-486 |
, |
denotes |
, |
T9811 |
486-498 |
JJ |
denotes |
non-specific |
T9812 |
507-512 |
NNS |
denotes |
sites |
T9813 |
499-506 |
NN |
denotes |
binding |
T9814 |
513-517 |
VBD |
denotes |
were |
T9815 |
526-528 |
IN |
denotes |
by |
T9816 |
529-539 |
VBG |
denotes |
incubating |
T9817 |
540-543 |
DT |
denotes |
the |
T9818 |
544-550 |
NNS |
denotes |
slides |
T9819 |
551-555 |
IN |
denotes |
with |
T9820 |
556-559 |
NN |
denotes |
PBG |
T9821 |
560-561 |
-LRB- |
denotes |
( |
T9822 |
567-570 |
NN |
denotes |
BSA |
T9823 |
561-564 |
CD |
denotes |
0.5 |
T9824 |
565-566 |
NN |
denotes |
% |
T9825 |
570-572 |
, |
denotes |
, |
T9826 |
572-577 |
CD |
denotes |
0.045 |
T9827 |
578-579 |
NN |
denotes |
% |
T9828 |
585-593 |
NN |
denotes |
gelatine |
T9829 |
580-584 |
NN |
denotes |
fish |
T9830 |
594-596 |
IN |
denotes |
in |
T9831 |
597-600 |
NN |
denotes |
PBS |
T9832 |
600-601 |
-RRB- |
denotes |
) |
T9833 |
602-604 |
IN |
denotes |
in |
T9834 |
605-607 |
CD |
denotes |
10 |
T9835 |
607-608 |
NN |
denotes |
% |
T9836 |
609-615 |
JJ |
denotes |
normal |
T9837 |
621-626 |
NN |
denotes |
serum |
T9838 |
616-620 |
NN |
denotes |
goat |
T9839 |
627-628 |
-LRB- |
denotes |
( |
T9840 |
628-631 |
NN |
denotes |
NGS |
T9841 |
631-632 |
-RRB- |
denotes |
) |
T9842 |
633-636 |
IN |
denotes |
for |
T9843 |
637-638 |
CD |
denotes |
2 |
T9844 |
639-640 |
NN |
denotes |
h |
T9845 |
640-641 |
. |
denotes |
. |
T9846 |
641-753 |
sentence |
denotes |
Incubation with the primary antibodies was performed at 4°C overnight diluted in PBG supplemented with 1 % NGS. |
T9847 |
642-652 |
NN |
denotes |
Incubation |
T9848 |
712-719 |
VBN |
denotes |
diluted |
T9849 |
653-657 |
IN |
denotes |
with |
T9850 |
658-661 |
DT |
denotes |
the |
T9851 |
670-680 |
NNS |
denotes |
antibodies |
T9852 |
662-669 |
JJ |
denotes |
primary |
T9853 |
681-684 |
VBD |
denotes |
was |
T9854 |
685-694 |
VBN |
denotes |
performed |
T9855 |
695-697 |
IN |
denotes |
at |
T9856 |
698-699 |
CD |
denotes |
4 |
T9857 |
699-701 |
NN |
denotes |
°C |
T9858 |
702-711 |
RB |
denotes |
overnight |
T9859 |
720-722 |
IN |
denotes |
in |
T9860 |
723-726 |
NN |
denotes |
PBG |
T9861 |
727-739 |
VBN |
denotes |
supplemented |
T9862 |
740-744 |
IN |
denotes |
with |
T9863 |
745-746 |
CD |
denotes |
1 |
T9864 |
747-748 |
NN |
denotes |
% |
T9865 |
749-752 |
NN |
denotes |
NGS |
T9866 |
752-753 |
. |
denotes |
. |
T9867 |
753-986 |
sentence |
denotes |
After five washes in PBG for 5 min each, the slides were incubated for 1 h with the secondary antibody at room temperature, washed three times in PBG and three times in PBS, 5 min each, rinsed in water and embedded in gelvatol [36]. |
T9868 |
754-759 |
IN |
denotes |
After |
T9869 |
811-820 |
VBN |
denotes |
incubated |
T9870 |
760-764 |
CD |
denotes |
five |
T9871 |
765-771 |
NNS |
denotes |
washes |
T9872 |
772-774 |
IN |
denotes |
in |
T9873 |
775-778 |
NN |
denotes |
PBG |
T9874 |
779-782 |
IN |
denotes |
for |
T9875 |
783-784 |
CD |
denotes |
5 |
T9876 |
785-788 |
NN |
denotes |
min |
T9877 |
789-793 |
DT |
denotes |
each |
T9878 |
793-795 |
, |
denotes |
, |
T9879 |
795-798 |
DT |
denotes |
the |
T9880 |
799-805 |
NNS |
denotes |
slides |
T9881 |
806-810 |
VBD |
denotes |
were |
T9882 |
821-824 |
IN |
denotes |
for |
T9883 |
825-826 |
CD |
denotes |
1 |
T9884 |
827-828 |
NN |
denotes |
h |
T9885 |
829-833 |
IN |
denotes |
with |
T9886 |
834-837 |
DT |
denotes |
the |
T9887 |
848-856 |
NN |
denotes |
antibody |
T9888 |
838-847 |
JJ |
denotes |
secondary |
T9889 |
857-859 |
IN |
denotes |
at |
T9890 |
860-864 |
NN |
denotes |
room |
T9891 |
865-876 |
NN |
denotes |
temperature |
T9892 |
876-878 |
, |
denotes |
, |
T9893 |
878-884 |
VBD |
denotes |
washed |
T9894 |
885-890 |
CD |
denotes |
three |
T9895 |
891-896 |
NNS |
denotes |
times |
T9896 |
897-899 |
IN |
denotes |
in |
T9897 |
900-903 |
NN |
denotes |
PBG |
T9898 |
904-907 |
CC |
denotes |
and |
T9899 |
908-913 |
CD |
denotes |
three |
T9900 |
914-919 |
NNS |
denotes |
times |
T9901 |
920-922 |
IN |
denotes |
in |
T9902 |
923-926 |
NN |
denotes |
PBS |
T9903 |
926-928 |
, |
denotes |
, |
T9904 |
928-929 |
CD |
denotes |
5 |
T9905 |
930-933 |
NNS |
denotes |
min |
T9906 |
934-938 |
DT |
denotes |
each |
T9907 |
938-940 |
, |
denotes |
, |
T9908 |
940-946 |
VBN |
denotes |
rinsed |
T9909 |
947-949 |
IN |
denotes |
in |
T9910 |
950-955 |
NN |
denotes |
water |
T9911 |
956-959 |
CC |
denotes |
and |
T9912 |
960-968 |
VBN |
denotes |
embedded |
T9913 |
969-971 |
IN |
denotes |
in |
T9914 |
972-980 |
NN |
denotes |
gelvatol |
T9915 |
981-982 |
-LRB- |
denotes |
[ |
T9916 |
982-984 |
CD |
denotes |
36 |
T9917 |
984-985 |
-RRB- |
denotes |
] |
T9918 |
985-986 |
. |
denotes |
. |
T9919 |
986-1127 |
sentence |
denotes |
For control, sections were incubated only with the secondary antibody or sections obtained from an annexin A7 knockout mouse were used [10]. |
T9920 |
987-990 |
IN |
denotes |
For |
T9921 |
1014-1023 |
VBN |
denotes |
incubated |
T9922 |
991-998 |
NN |
denotes |
control |
T9923 |
998-1000 |
, |
denotes |
, |
T9924 |
1000-1008 |
NNS |
denotes |
sections |
T9925 |
1009-1013 |
VBD |
denotes |
were |
T9926 |
1024-1028 |
RB |
denotes |
only |
T9927 |
1029-1033 |
IN |
denotes |
with |
T9928 |
1034-1037 |
DT |
denotes |
the |
T9929 |
1048-1056 |
NN |
denotes |
antibody |
T9930 |
1038-1047 |
JJ |
denotes |
secondary |
T9931 |
1057-1059 |
CC |
denotes |
or |
T9932 |
1060-1068 |
NNS |
denotes |
sections |
T9933 |
1117-1121 |
VBN |
denotes |
used |
T9934 |
1069-1077 |
VBN |
denotes |
obtained |
T9935 |
1078-1082 |
IN |
denotes |
from |
T9936 |
1083-1085 |
DT |
denotes |
an |
T9937 |
1106-1111 |
NN |
denotes |
mouse |
T9938 |
1086-1093 |
NN |
denotes |
annexin |
T9939 |
1094-1096 |
NN |
denotes |
A7 |
T9940 |
1097-1105 |
NN |
denotes |
knockout |
T9941 |
1112-1116 |
VBD |
denotes |
were |
T9942 |
1122-1123 |
-LRB- |
denotes |
[ |
T9943 |
1123-1125 |
CD |
denotes |
10 |
T9944 |
1125-1126 |
-RRB- |
denotes |
] |
T9945 |
1126-1127 |
. |
denotes |
. |
T9946 |
1127-1225 |
sentence |
denotes |
Samples were examined with a confocal laser scan microscope (Leica TCS SP; Leica, Bensheim, FRG). |
T9947 |
1128-1135 |
NNS |
denotes |
Samples |
T9948 |
1141-1149 |
VBN |
denotes |
examined |
T9949 |
1136-1140 |
VBD |
denotes |
were |
T9950 |
1150-1154 |
IN |
denotes |
with |
T9951 |
1155-1156 |
DT |
denotes |
a |
T9952 |
1177-1187 |
NN |
denotes |
microscope |
T9953 |
1157-1165 |
JJ |
denotes |
confocal |
T9954 |
1166-1171 |
NN |
denotes |
laser |
T9955 |
1172-1176 |
NN |
denotes |
scan |
T9956 |
1188-1189 |
-LRB- |
denotes |
( |
T9957 |
1199-1201 |
NN |
denotes |
SP |
T9958 |
1189-1194 |
NNP |
denotes |
Leica |
T9959 |
1195-1198 |
NN |
denotes |
TCS |
T9960 |
1201-1202 |
: |
denotes |
; |
T9961 |
1203-1208 |
NNP |
denotes |
Leica |
T9962 |
1208-1210 |
, |
denotes |
, |
T9963 |
1210-1218 |
NNP |
denotes |
Bensheim |
T9964 |
1218-1220 |
, |
denotes |
, |
T9965 |
1220-1223 |
NNP |
denotes |
FRG |
T9966 |
1223-1224 |
-RRB- |
denotes |
) |
T9967 |
1224-1225 |
. |
denotes |
. |
R4650 |
T9710 |
T9711 |
det |
The,sections |
R4651 |
T9711 |
T9715 |
nsubjpass |
sections,treated |
R4652 |
T9712 |
T9713 |
npadvmod |
paraffin,embedded |
R4653 |
T9713 |
T9711 |
amod |
embedded,sections |
R4654 |
T9714 |
T9713 |
punct |
-,embedded |
R4655 |
T9716 |
T9715 |
auxpass |
were,treated |
R4656 |
T9717 |
T9715 |
advmod |
first,treated |
R4657 |
T9718 |
T9719 |
nummod |
three,times |
R4658 |
T9719 |
T9715 |
npadvmod |
times,treated |
R4659 |
T9720 |
T9715 |
prep |
for,treated |
R4660 |
T9721 |
T9722 |
nummod |
2,min |
R4661 |
T9722 |
T9720 |
pobj |
min,for |
R4662 |
T9723 |
T9715 |
prep |
in,treated |
R4663 |
T9724 |
T9723 |
pobj |
xylene,in |
R4664 |
T9725 |
T9726 |
aux |
to,remove |
R4665 |
T9726 |
T9715 |
advcl |
remove,treated |
R4666 |
T9727 |
T9726 |
dobj |
paraffin,remove |
R4667 |
T9728 |
T9715 |
cc |
and,treated |
R4668 |
T9729 |
T9730 |
advmod |
then,rehydrated |
R4669 |
T9730 |
T9715 |
conj |
rehydrated,treated |
R4670 |
T9731 |
T9730 |
npadvmod |
step,rehydrated |
R4671 |
T9732 |
T9731 |
prep |
by,step |
R4672 |
T9733 |
T9732 |
pobj |
step,by |
R4673 |
T9734 |
T9730 |
prep |
with,rehydrated |
R4674 |
T9735 |
T9736 |
amod |
descending,concentrations |
R4675 |
T9736 |
T9734 |
pobj |
concentrations,with |
R4676 |
T9737 |
T9736 |
prep |
of,concentrations |
R4677 |
T9738 |
T9737 |
pobj |
ethanol,of |
R4678 |
T9739 |
T9730 |
prep |
for,rehydrated |
R4679 |
T9740 |
T9741 |
compound |
antibody,staining |
R4680 |
T9741 |
T9739 |
pobj |
staining,for |
R4681 |
T9742 |
T9743 |
punct |
[,35 |
R4682 |
T9743 |
T9730 |
parataxis |
35,rehydrated |
R4683 |
T9744 |
T9743 |
punct |
],35 |
R4684 |
T9745 |
T9715 |
punct |
.,treated |
R4685 |
T9747 |
T9748 |
prep |
After,incubated |
R4686 |
T9749 |
T9750 |
nummod |
three,washings |
R4687 |
T9750 |
T9747 |
pobj |
washings,After |
R4688 |
T9751 |
T9750 |
prep |
in,washings |
R4689 |
T9752 |
T9751 |
pobj |
PBS,in |
R4690 |
T9753 |
T9754 |
punct |
(,NaCl |
R4691 |
T9754 |
T9750 |
parataxis |
NaCl,washings |
R4692 |
T9755 |
T9756 |
nummod |
137,mM |
R4693 |
T9756 |
T9754 |
compound |
mM,NaCl |
R4694 |
T9757 |
T9754 |
punct |
", ",NaCl |
R4695 |
T9758 |
T9759 |
nummod |
2.7,mM |
R4696 |
T9759 |
T9760 |
compound |
mM,KCl |
R4697 |
T9760 |
T9754 |
appos |
KCl,NaCl |
R4698 |
T9761 |
T9754 |
punct |
", ",NaCl |
R4699 |
T9762 |
T9763 |
nummod |
8.1,mM |
R4700 |
T9763 |
T9764 |
compound |
mM,Na2HPO4 |
R4701 |
T9764 |
T9754 |
appos |
Na2HPO4,NaCl |
R4702 |
T9765 |
T9754 |
punct |
", ",NaCl |
R4703 |
T9766 |
T9767 |
nummod |
1.5,mM |
R4704 |
T9767 |
T9768 |
compound |
mM,KH2PO4 |
R4705 |
T9768 |
T9754 |
appos |
KH2PO4,NaCl |
R4706 |
T9769 |
T9754 |
punct |
", ",NaCl |
R4707 |
T9770 |
T9754 |
npadvmod |
pH,NaCl |
R4708 |
T9771 |
T9770 |
nummod |
7.4,pH |
R4709 |
T9772 |
T9754 |
punct |
),NaCl |
R4710 |
T9773 |
T9748 |
punct |
", ",incubated |
R4711 |
T9774 |
T9775 |
det |
the,sections |
R4712 |
T9775 |
T9748 |
nsubjpass |
sections,incubated |
R4713 |
T9776 |
T9775 |
compound |
tissue,sections |
R4714 |
T9777 |
T9748 |
auxpass |
were,incubated |
R4715 |
T9778 |
T9748 |
prep |
in,incubated |
R4716 |
T9779 |
T9778 |
pobj |
PBS,in |
R4717 |
T9780 |
T9779 |
acl |
containing,PBS |
R4718 |
T9781 |
T9782 |
nummod |
0.1,% |
R4719 |
T9782 |
T9783 |
compound |
%,trypsin |
R4720 |
T9783 |
T9780 |
dobj |
trypsin,containing |
R4721 |
T9784 |
T9748 |
prep |
for,incubated |
R4722 |
T9785 |
T9786 |
nummod |
10,min |
R4723 |
T9786 |
T9784 |
pobj |
min,for |
R4724 |
T9787 |
T9748 |
prep |
at,incubated |
R4725 |
T9788 |
T9789 |
compound |
room,temperature |
R4726 |
T9789 |
T9787 |
pobj |
temperature,at |
R4727 |
T9790 |
T9791 |
aux |
to,free |
R4728 |
T9791 |
T9748 |
advcl |
free,incubated |
R4729 |
T9792 |
T9793 |
npadvmod |
formaldehyde,crosslinked |
R4730 |
T9793 |
T9795 |
amod |
crosslinked,epitopes |
R4731 |
T9794 |
T9793 |
punct |
-,crosslinked |
R4732 |
T9795 |
T9791 |
dobj |
epitopes,free |
R4733 |
T9796 |
T9797 |
punct |
[,15 |
R4734 |
T9797 |
T9748 |
parataxis |
15,incubated |
R4735 |
T9798 |
T9799 |
punct |
-,17 |
R4736 |
T9799 |
T9797 |
prep |
17,15 |
R4737 |
T9800 |
T9797 |
punct |
],15 |
R4738 |
T9801 |
T9748 |
punct |
.,incubated |
R4739 |
T9803 |
T9804 |
prep |
After,blocked |
R4740 |
T9805 |
T9806 |
amod |
additional,washes |
R4741 |
T9806 |
T9803 |
pobj |
washes,After |
R4742 |
T9807 |
T9806 |
nummod |
three,washes |
R4743 |
T9808 |
T9806 |
prep |
with,washes |
R4744 |
T9809 |
T9808 |
pobj |
PBS,with |
R4745 |
T9810 |
T9804 |
punct |
", ",blocked |
R4746 |
T9811 |
T9812 |
amod |
non-specific,sites |
R4747 |
T9812 |
T9804 |
nsubjpass |
sites,blocked |
R4748 |
T9813 |
T9812 |
compound |
binding,sites |
R4749 |
T9814 |
T9804 |
auxpass |
were,blocked |
R4750 |
T9815 |
T9804 |
prep |
by,blocked |
R4751 |
T9816 |
T9815 |
pcomp |
incubating,by |
R4752 |
T9817 |
T9818 |
det |
the,slides |
R4753 |
T9818 |
T9816 |
dobj |
slides,incubating |
R4754 |
T9819 |
T9816 |
prep |
with,incubating |
R4755 |
T9820 |
T9819 |
pobj |
PBG,with |
R4756 |
T9821 |
T9822 |
punct |
(,BSA |
R4757 |
T9822 |
T9820 |
parataxis |
BSA,PBG |
R4758 |
T9823 |
T9824 |
nummod |
0.5,% |
R4759 |
T9824 |
T9822 |
compound |
%,BSA |
R4760 |
T9825 |
T9822 |
punct |
", ",BSA |
R4761 |
T9826 |
T9827 |
nummod |
0.045,% |
R4762 |
T9827 |
T9828 |
compound |
%,gelatine |
R4763 |
T9828 |
T9822 |
appos |
gelatine,BSA |
R4764 |
T9829 |
T9828 |
compound |
fish,gelatine |
R4765 |
T9830 |
T9822 |
prep |
in,BSA |
R4766 |
T9831 |
T9830 |
pobj |
PBS,in |
R4767 |
T9832 |
T9822 |
punct |
),BSA |
R4768 |
T9833 |
T9816 |
prep |
in,incubating |
R4769 |
T9834 |
T9835 |
nummod |
10,% |
R4770 |
T9835 |
T9833 |
pobj |
%,in |
R4771 |
T9836 |
T9837 |
amod |
normal,serum |
R4772 |
T9837 |
T9835 |
appos |
serum,% |
R4773 |
T9838 |
T9837 |
compound |
goat,serum |
R4774 |
T9839 |
T9837 |
punct |
(,serum |
R4775 |
T9840 |
T9837 |
appos |
NGS,serum |
R4776 |
T9841 |
T9835 |
punct |
),% |
R4777 |
T9842 |
T9816 |
prep |
for,incubating |
R4778 |
T9843 |
T9844 |
nummod |
2,h |
R4779 |
T9844 |
T9842 |
pobj |
h,for |
R4780 |
T9845 |
T9804 |
punct |
.,blocked |
R4781 |
T9847 |
T9848 |
nsubjpass |
Incubation,diluted |
R4782 |
T9849 |
T9847 |
prep |
with,Incubation |
R4783 |
T9850 |
T9851 |
det |
the,antibodies |
R4784 |
T9851 |
T9849 |
pobj |
antibodies,with |
R4785 |
T9852 |
T9851 |
amod |
primary,antibodies |
R4786 |
T9853 |
T9848 |
auxpass |
was,diluted |
R4787 |
T9854 |
T9848 |
aux |
performed,diluted |
R4788 |
T9855 |
T9848 |
prep |
at,diluted |
R4789 |
T9856 |
T9857 |
nummod |
4,°C |
R4790 |
T9857 |
T9855 |
pobj |
°C,at |
R4791 |
T9858 |
T9848 |
advmod |
overnight,diluted |
R4792 |
T9859 |
T9848 |
prep |
in,diluted |
R4793 |
T9860 |
T9859 |
pobj |
PBG,in |
R4794 |
T9861 |
T9860 |
acl |
supplemented,PBG |
R4795 |
T9862 |
T9861 |
prep |
with,supplemented |
R4796 |
T9863 |
T9864 |
nummod |
1,% |
R4797 |
T9864 |
T9865 |
compound |
%,NGS |
R4798 |
T9865 |
T9862 |
pobj |
NGS,with |
R4799 |
T9866 |
T9848 |
punct |
.,diluted |
R4800 |
T9868 |
T9869 |
prep |
After,incubated |
R4801 |
T9870 |
T9871 |
nummod |
five,washes |
R4802 |
T9871 |
T9868 |
pobj |
washes,After |
R4803 |
T9872 |
T9871 |
prep |
in,washes |
R4804 |
T9873 |
T9872 |
pobj |
PBG,in |
R4805 |
T9874 |
T9871 |
prep |
for,washes |
R4806 |
T9875 |
T9876 |
nummod |
5,min |
R4807 |
T9876 |
T9874 |
pobj |
min,for |
R4808 |
T9877 |
T9876 |
appos |
each,min |
R4809 |
T9878 |
T9869 |
punct |
", ",incubated |
R4810 |
T9879 |
T9880 |
det |
the,slides |
R4811 |
T9880 |
T9869 |
nsubjpass |
slides,incubated |
R4812 |
T9881 |
T9869 |
auxpass |
were,incubated |
R4813 |
T9882 |
T9869 |
prep |
for,incubated |
R4814 |
T9883 |
T9884 |
nummod |
1,h |
R4815 |
T9884 |
T9882 |
pobj |
h,for |
R4816 |
T9885 |
T9869 |
prep |
with,incubated |
R4817 |
T9886 |
T9887 |
det |
the,antibody |
R4818 |
T9887 |
T9885 |
pobj |
antibody,with |
R4819 |
T9888 |
T9887 |
amod |
secondary,antibody |
R4820 |
T9889 |
T9869 |
prep |
at,incubated |
R4821 |
T9890 |
T9891 |
compound |
room,temperature |
R4822 |
T9891 |
T9889 |
pobj |
temperature,at |
R4823 |
T9892 |
T9869 |
punct |
", ",incubated |
R4824 |
T9893 |
T9869 |
conj |
washed,incubated |
R4825 |
T9894 |
T9895 |
nummod |
three,times |
R4826 |
T9895 |
T9893 |
npadvmod |
times,washed |
R4827 |
T9896 |
T9893 |
prep |
in,washed |
R4828 |
T9897 |
T9896 |
pobj |
PBG,in |
R4829 |
T9898 |
T9893 |
cc |
and,washed |
R4830 |
T9899 |
T9900 |
nummod |
three,times |
R4831 |
T9900 |
T9893 |
conj |
times,washed |
R4832 |
T9901 |
T9900 |
prep |
in,times |
R4833 |
T9902 |
T9901 |
pobj |
PBS,in |
R4834 |
T9903 |
T9893 |
punct |
", ",washed |
R4835 |
T9904 |
T9905 |
nummod |
5,min |
R4836 |
T9905 |
T9893 |
npadvmod |
min,washed |
R4837 |
T9906 |
T9905 |
appos |
each,min |
R4838 |
T9907 |
T9893 |
punct |
", ",washed |
R4839 |
T9908 |
T9893 |
conj |
rinsed,washed |
R4840 |
T9909 |
T9908 |
prep |
in,rinsed |
R4841 |
T9910 |
T9909 |
pobj |
water,in |
R4842 |
T9911 |
T9908 |
cc |
and,rinsed |
R4843 |
T9912 |
T9908 |
conj |
embedded,rinsed |
R4844 |
T9913 |
T9912 |
prep |
in,embedded |
R4845 |
T9914 |
T9913 |
pobj |
gelvatol,in |
R4846 |
T9915 |
T9916 |
punct |
[,36 |
R4847 |
T9916 |
T9912 |
parataxis |
36,embedded |
R4848 |
T9917 |
T9916 |
punct |
],36 |
R4849 |
T9918 |
T9869 |
punct |
.,incubated |
R4850 |
T9920 |
T9921 |
prep |
For,incubated |
R4851 |
T9922 |
T9920 |
pobj |
control,For |
R4852 |
T9923 |
T9921 |
punct |
", ",incubated |
R4853 |
T9924 |
T9921 |
nsubjpass |
sections,incubated |
R4854 |
T9925 |
T9921 |
auxpass |
were,incubated |
R4855 |
T9926 |
T9927 |
advmod |
only,with |
R4856 |
T9927 |
T9921 |
prep |
with,incubated |
R4857 |
T9928 |
T9929 |
det |
the,antibody |
R4858 |
T9929 |
T9927 |
pobj |
antibody,with |
R4859 |
T9930 |
T9929 |
amod |
secondary,antibody |
R4860 |
T9931 |
T9921 |
cc |
or,incubated |
R4861 |
T9932 |
T9933 |
nsubjpass |
sections,used |
R4862 |
T9933 |
T9921 |
conj |
used,incubated |
R4863 |
T9934 |
T9932 |
acl |
obtained,sections |
R4864 |
T9935 |
T9934 |
prep |
from,obtained |
R4865 |
T9936 |
T9937 |
det |
an,mouse |
R4866 |
T9937 |
T9935 |
pobj |
mouse,from |
R4867 |
T9938 |
T9939 |
compound |
annexin,A7 |
R4868 |
T9939 |
T9937 |
compound |
A7,mouse |
R4869 |
T9940 |
T9937 |
compound |
knockout,mouse |
R4870 |
T9941 |
T9933 |
auxpass |
were,used |
R4871 |
T9942 |
T9943 |
punct |
[,10 |
R4872 |
T9943 |
T9933 |
parataxis |
10,used |
R4873 |
T9944 |
T9943 |
punct |
],10 |
R4874 |
T9945 |
T9921 |
punct |
.,incubated |
R4875 |
T9947 |
T9948 |
nsubjpass |
Samples,examined |
R4876 |
T9949 |
T9948 |
auxpass |
were,examined |
R4877 |
T9950 |
T9948 |
prep |
with,examined |
R4878 |
T9951 |
T9952 |
det |
a,microscope |
R4879 |
T9952 |
T9950 |
pobj |
microscope,with |
R4880 |
T9953 |
T9952 |
amod |
confocal,microscope |
R4881 |
T9954 |
T9952 |
compound |
laser,microscope |
R4882 |
T9955 |
T9952 |
compound |
scan,microscope |
R4883 |
T9956 |
T9957 |
punct |
(,SP |
R4884 |
T9957 |
T9952 |
parataxis |
SP,microscope |
R4885 |
T9958 |
T9957 |
compound |
Leica,SP |
R4886 |
T9959 |
T9957 |
compound |
TCS,SP |
R4887 |
T9960 |
T9957 |
punct |
;,SP |
R4888 |
T9961 |
T9957 |
appos |
Leica,SP |
R4889 |
T9962 |
T9961 |
punct |
", ",Leica |
R4890 |
T9963 |
T9961 |
npadvmod |
Bensheim,Leica |
R4891 |
T9964 |
T9961 |
punct |
", ",Leica |
R4892 |
T9965 |
T9961 |
npadvmod |
FRG,Leica |
R4893 |
T9966 |
T9957 |
punct |
),SP |
R4894 |
T9967 |
T9948 |
punct |
.,examined |