PMC:1064873 / 14998-17327 JSON TXT

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LappsTest

2_test

{"project":"2_test","denotations":[{"id":"15535835-10898505-4156888","span":{"begin":307,"end":309},"obj":"10898505"},{"id":"15535835-7306232-4156889","span":{"begin":1243,"end":1245},"obj":"7306232"}],"text":"Resistance to IL-10 inhibition of IFN-γ production in RA CD4+ T cells\nThe CD28 costimulatory pathway is crucial for effective antigen-specific T-cell cytokine production, and IL-10 can directly suppress this response by inhibiting CD28 tyrosine phosphorylation and binding of phosphatidylinositol 3-kinase [24]. To evaluate the responsiveness of RA CD4+ T cells to IL-10, purified PB CD4+ T cells from three patients with active RA and from three healthy controls were stimulated by immobilized anti-CD3 antibody and anti-CD28 antibody with or without diluted concentrations of IL-10 for 36 hours, and IFN-γ production was measured by ELISA. As shown in Fig. 1, IFN-γ production by activated normal CD4+ T cells was mostly inhibited at concentrations as low as 1 ng/ml IL-10. However, RA CD4+ T cells were able to produce significant amounts of IFN-γ in the presence of 1 ng/ml IL-10, and the maximal but not complete inhibition by IL-10 was obtained at 10–100 ng/ml.\nWe thus compared the levels of IFN-γ production by CD4+ T cells after CD3 and CD28 costimulation in the presence of 1 ng/ml IL-10 in RA patients with active disease (multiple inflammatory joints, CRP level ≥ 10 mg/l) and inactive disease (in remission, CRP level \u003c 10 mg/l) [26] and in healthy controls. There were no statistically significant differences in IFN-γ production without IL-10 among these three groups (Fig. 2a), but the inhibitory effect of IL-10 on IFN-γ production was significantly limited in the active RA group as compared with the inactive RA group and healthy controls (percentage decrease: active RA, 2.9 ± 14.4%; inactive RA, 45.6 ± 14.4%; controls, 65.8 ± 7.9%) (Fig. 2b). As a consequence, CD4+ T cells from active RA patients produced higher levels of IFN-γ in the presence of 1 ng/ml IL-10 than did normal CD4+ T cells (Fig. 2a).\nIn addition, we compared IL-2 production by CD4+ T cells after CD3 and CD28 costimulation in the presence of IL-10 in active RA patients and in healthy controls. Similarly, IL-2 production was not affected by 1 ng/ml IL-10 in RA patients (percentage decrease, -2.1 ± 13.8%), while it was significantly reduced in healthy controls (61.1 ± 13.7%; P \u003c 0.05). Taken together, these results indicate that RA CD4+ T cells become less susceptible to the immunoregulatory effect of IL-10 during the active phase."}

biosemtest

pmc-enju-pas

bionlp-st-ge-2016-test-proteins

bionlp-st-ge-2016-uniprot

GO-BP

GO-CC

{"project":"GO-CC","denotations":[{"id":"T12964","span":{"begin":527,"end":535},"obj":"http://purl.obolibrary.org/obo/GO_0042571"},{"id":"T12963","span":{"begin":504,"end":512},"obj":"http://purl.obolibrary.org/obo/GO_0042571"},{"id":"T12962","span":{"begin":527,"end":535},"obj":"http://purl.obolibrary.org/obo/GO_0019815"},{"id":"T12961","span":{"begin":504,"end":512},"obj":"http://purl.obolibrary.org/obo/GO_0019815"},{"id":"T12960","span":{"begin":2235,"end":2240},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T12959","span":{"begin":1876,"end":1881},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T12958","span":{"begin":1808,"end":1813},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T12957","span":{"begin":1690,"end":1695},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T12956","span":{"begin":1026,"end":1031},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T12955","span":{"begin":795,"end":800},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T12954","span":{"begin":706,"end":711},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T12953","span":{"begin":391,"end":396},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T12952","span":{"begin":356,"end":361},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T12951","span":{"begin":64,"end":69},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"Resistance to IL-10 inhibition of IFN-γ production in RA CD4+ T cells\nThe CD28 costimulatory pathway is crucial for effective antigen-specific T-cell cytokine production, and IL-10 can directly suppress this response by inhibiting CD28 tyrosine phosphorylation and binding of phosphatidylinositol 3-kinase [24]. To evaluate the responsiveness of RA CD4+ T cells to IL-10, purified PB CD4+ T cells from three patients with active RA and from three healthy controls were stimulated by immobilized anti-CD3 antibody and anti-CD28 antibody with or without diluted concentrations of IL-10 for 36 hours, and IFN-γ production was measured by ELISA. As shown in Fig. 1, IFN-γ production by activated normal CD4+ T cells was mostly inhibited at concentrations as low as 1 ng/ml IL-10. However, RA CD4+ T cells were able to produce significant amounts of IFN-γ in the presence of 1 ng/ml IL-10, and the maximal but not complete inhibition by IL-10 was obtained at 10–100 ng/ml.\nWe thus compared the levels of IFN-γ production by CD4+ T cells after CD3 and CD28 costimulation in the presence of 1 ng/ml IL-10 in RA patients with active disease (multiple inflammatory joints, CRP level ≥ 10 mg/l) and inactive disease (in remission, CRP level \u003c 10 mg/l) [26] and in healthy controls. There were no statistically significant differences in IFN-γ production without IL-10 among these three groups (Fig. 2a), but the inhibitory effect of IL-10 on IFN-γ production was significantly limited in the active RA group as compared with the inactive RA group and healthy controls (percentage decrease: active RA, 2.9 ± 14.4%; inactive RA, 45.6 ± 14.4%; controls, 65.8 ± 7.9%) (Fig. 2b). As a consequence, CD4+ T cells from active RA patients produced higher levels of IFN-γ in the presence of 1 ng/ml IL-10 than did normal CD4+ T cells (Fig. 2a).\nIn addition, we compared IL-2 production by CD4+ T cells after CD3 and CD28 costimulation in the presence of IL-10 in active RA patients and in healthy controls. Similarly, IL-2 production was not affected by 1 ng/ml IL-10 in RA patients (percentage decrease, -2.1 ± 13.8%), while it was significantly reduced in healthy controls (61.1 ± 13.7%; P \u003c 0.05). Taken together, these results indicate that RA CD4+ T cells become less susceptible to the immunoregulatory effect of IL-10 during the active phase."}

GO-MF

sentences

{"project":"sentences","denotations":[{"id":"T12806","span":{"begin":2181,"end":2329},"obj":"Sentence"},{"id":"T12805","span":{"begin":1987,"end":2180},"obj":"Sentence"},{"id":"T12804","span":{"begin":1825,"end":1986},"obj":"Sentence"},{"id":"T12803","span":{"begin":1665,"end":1824},"obj":"Sentence"},{"id":"T12802","span":{"begin":1272,"end":1664},"obj":"Sentence"},{"id":"T12801","span":{"begin":968,"end":1271},"obj":"Sentence"},{"id":"T12800","span":{"begin":776,"end":967},"obj":"Sentence"},{"id":"T12799","span":{"begin":642,"end":775},"obj":"Sentence"},{"id":"T12798","span":{"begin":312,"end":641},"obj":"Sentence"},{"id":"T12797","span":{"begin":70,"end":311},"obj":"Sentence"},{"id":"T12796","span":{"begin":0,"end":69},"obj":"Sentence"},{"id":"T92","span":{"begin":0,"end":69},"obj":"Sentence"},{"id":"T93","span":{"begin":70,"end":311},"obj":"Sentence"},{"id":"T94","span":{"begin":312,"end":641},"obj":"Sentence"},{"id":"T95","span":{"begin":642,"end":775},"obj":"Sentence"},{"id":"T96","span":{"begin":776,"end":967},"obj":"Sentence"},{"id":"T97","span":{"begin":968,"end":1271},"obj":"Sentence"},{"id":"T98","span":{"begin":1272,"end":1664},"obj":"Sentence"},{"id":"T99","span":{"begin":1665,"end":1824},"obj":"Sentence"},{"id":"T100","span":{"begin":1825,"end":1986},"obj":"Sentence"},{"id":"T101","span":{"begin":1987,"end":2180},"obj":"Sentence"},{"id":"T102","span":{"begin":2181,"end":2329},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Resistance to IL-10 inhibition of IFN-γ production in RA CD4+ T cells\nThe CD28 costimulatory pathway is crucial for effective antigen-specific T-cell cytokine production, and IL-10 can directly suppress this response by inhibiting CD28 tyrosine phosphorylation and binding of phosphatidylinositol 3-kinase [24]. To evaluate the responsiveness of RA CD4+ T cells to IL-10, purified PB CD4+ T cells from three patients with active RA and from three healthy controls were stimulated by immobilized anti-CD3 antibody and anti-CD28 antibody with or without diluted concentrations of IL-10 for 36 hours, and IFN-γ production was measured by ELISA. As shown in Fig. 1, IFN-γ production by activated normal CD4+ T cells was mostly inhibited at concentrations as low as 1 ng/ml IL-10. However, RA CD4+ T cells were able to produce significant amounts of IFN-γ in the presence of 1 ng/ml IL-10, and the maximal but not complete inhibition by IL-10 was obtained at 10–100 ng/ml.\nWe thus compared the levels of IFN-γ production by CD4+ T cells after CD3 and CD28 costimulation in the presence of 1 ng/ml IL-10 in RA patients with active disease (multiple inflammatory joints, CRP level ≥ 10 mg/l) and inactive disease (in remission, CRP level \u003c 10 mg/l) [26] and in healthy controls. There were no statistically significant differences in IFN-γ production without IL-10 among these three groups (Fig. 2a), but the inhibitory effect of IL-10 on IFN-γ production was significantly limited in the active RA group as compared with the inactive RA group and healthy controls (percentage decrease: active RA, 2.9 ± 14.4%; inactive RA, 45.6 ± 14.4%; controls, 65.8 ± 7.9%) (Fig. 2b). As a consequence, CD4+ T cells from active RA patients produced higher levels of IFN-γ in the presence of 1 ng/ml IL-10 than did normal CD4+ T cells (Fig. 2a).\nIn addition, we compared IL-2 production by CD4+ T cells after CD3 and CD28 costimulation in the presence of IL-10 in active RA patients and in healthy controls. Similarly, IL-2 production was not affected by 1 ng/ml IL-10 in RA patients (percentage decrease, -2.1 ± 13.8%), while it was significantly reduced in healthy controls (61.1 ± 13.7%; P \u003c 0.05). Taken together, these results indicate that RA CD4+ T cells become less susceptible to the immunoregulatory effect of IL-10 during the active phase."}

simple1

BioNLP16_DUT

BioNLP16_Messiy

DLUT931

bionlp-st-ge-2016-test-ihmc

bionlp-st-ge-2016-spacy-parsed

bionlp-st-ge-2016-test-tees

test3

testone

PMC:1064873 / 14998-17327 JSONTXT

Annnotations TAB JSON ListView MergeView

PMC:1064873 / 14998-17327 JSON TXT