PMC:1064873 / 11394-12083 JSON TXT

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LappsTest

{"project":"LappsTest","denotations":[{"id":"T9321","span":{"begin":505,"end":523},"obj":"Protein"},{"id":"T9320","span":{"begin":214,"end":264},"obj":"Protein"},{"id":"T9319","span":{"begin":38,"end":45},"obj":"Protein"}],"text":"Total cellular RNA was extracted from PB CD4+ T cells using an RNA isolation kit (RNeasy Mini kit; Qiagen, Valencia, CA, USA), according to the manufacturer's instructions. cDNA was synthesized from total RNA with Molony murine leukemia virus reverse transcriptase (US Biochemical, Cleveland, OH, USA) and oligo-(dT)15 primers (Promega, Madison, WI, USA). Real-time PCR was performed with the LightCycler Instrument (Roche Diagnostics, Penzberg, Germany) in glass capillaries. The reaction mix containing Taq DNA polymerase and DNA double-strand-specific SYBR Green I dye (Lightcycler FastStart DNA Master SYBR Green I; Roche Diagnostics) and specific primers were added to cDNA dilutions."}

biosemtest

pmc-enju-pas

bionlp-st-ge-2016-test-proteins

{"project":"bionlp-st-ge-2016-test-proteins","denotations":[{"id":"T9751","span":{"begin":505,"end":508},"obj":"Protein"},{"id":"T9750","span":{"begin":41,"end":44},"obj":"Protein"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"Total cellular RNA was extracted from PB CD4+ T cells using an RNA isolation kit (RNeasy Mini kit; Qiagen, Valencia, CA, USA), according to the manufacturer's instructions. cDNA was synthesized from total RNA with Molony murine leukemia virus reverse transcriptase (US Biochemical, Cleveland, OH, USA) and oligo-(dT)15 primers (Promega, Madison, WI, USA). Real-time PCR was performed with the LightCycler Instrument (Roche Diagnostics, Penzberg, Germany) in glass capillaries. The reaction mix containing Taq DNA polymerase and DNA double-strand-specific SYBR Green I dye (Lightcycler FastStart DNA Master SYBR Green I; Roche Diagnostics) and specific primers were added to cDNA dilutions."}

bionlp-st-ge-2016-uniprot

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T9791","span":{"begin":41,"end":44},"obj":"http://www.uniprot.org/uniprot/P01730"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"Total cellular RNA was extracted from PB CD4+ T cells using an RNA isolation kit (RNeasy Mini kit; Qiagen, Valencia, CA, USA), according to the manufacturer's instructions. cDNA was synthesized from total RNA with Molony murine leukemia virus reverse transcriptase (US Biochemical, Cleveland, OH, USA) and oligo-(dT)15 primers (Promega, Madison, WI, USA). Real-time PCR was performed with the LightCycler Instrument (Roche Diagnostics, Penzberg, Germany) in glass capillaries. The reaction mix containing Taq DNA polymerase and DNA double-strand-specific SYBR Green I dye (Lightcycler FastStart DNA Master SYBR Green I; Roche Diagnostics) and specific primers were added to cDNA dilutions."}

UBERON-AE

{"project":"UBERON-AE","denotations":[{"id":"T9355","span":{"begin":464,"end":475},"obj":"http://purl.obolibrary.org/obo/UBERON_0001982"}],"text":"Total cellular RNA was extracted from PB CD4+ T cells using an RNA isolation kit (RNeasy Mini kit; Qiagen, Valencia, CA, USA), according to the manufacturer's instructions. cDNA was synthesized from total RNA with Molony murine leukemia virus reverse transcriptase (US Biochemical, Cleveland, OH, USA) and oligo-(dT)15 primers (Promega, Madison, WI, USA). Real-time PCR was performed with the LightCycler Instrument (Roche Diagnostics, Penzberg, Germany) in glass capillaries. The reaction mix containing Taq DNA polymerase and DNA double-strand-specific SYBR Green I dye (Lightcycler FastStart DNA Master SYBR Green I; Roche Diagnostics) and specific primers were added to cDNA dilutions."}

GO-BP

{"project":"GO-BP","denotations":[{"id":"T9735","span":{"begin":251,"end":264},"obj":"http://purl.obolibrary.org/obo/GO_0034062"},{"id":"T9734","span":{"begin":251,"end":264},"obj":"http://purl.obolibrary.org/obo/GO_0003968"},{"id":"T9733","span":{"begin":251,"end":264},"obj":"http://purl.obolibrary.org/obo/GO_0003899"},{"id":"T9732","span":{"begin":117,"end":119},"obj":"http://purl.obolibrary.org/obo/GO_0033968"},{"id":"T9731","span":{"begin":6,"end":14},"obj":"http://purl.obolibrary.org/obo/GO_0007349"}],"text":"Total cellular RNA was extracted from PB CD4+ T cells using an RNA isolation kit (RNeasy Mini kit; Qiagen, Valencia, CA, USA), according to the manufacturer's instructions. cDNA was synthesized from total RNA with Molony murine leukemia virus reverse transcriptase (US Biochemical, Cleveland, OH, USA) and oligo-(dT)15 primers (Promega, Madison, WI, USA). Real-time PCR was performed with the LightCycler Instrument (Roche Diagnostics, Penzberg, Germany) in glass capillaries. The reaction mix containing Taq DNA polymerase and DNA double-strand-specific SYBR Green I dye (Lightcycler FastStart DNA Master SYBR Green I; Roche Diagnostics) and specific primers were added to cDNA dilutions."}

GO-CC

{"project":"GO-CC","denotations":[{"id":"T9765","span":{"begin":48,"end":53},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"Total cellular RNA was extracted from PB CD4+ T cells using an RNA isolation kit (RNeasy Mini kit; Qiagen, Valencia, CA, USA), according to the manufacturer's instructions. cDNA was synthesized from total RNA with Molony murine leukemia virus reverse transcriptase (US Biochemical, Cleveland, OH, USA) and oligo-(dT)15 primers (Promega, Madison, WI, USA). Real-time PCR was performed with the LightCycler Instrument (Roche Diagnostics, Penzberg, Germany) in glass capillaries. The reaction mix containing Taq DNA polymerase and DNA double-strand-specific SYBR Green I dye (Lightcycler FastStart DNA Master SYBR Green I; Roche Diagnostics) and specific primers were added to cDNA dilutions."}

GO-MF

{"project":"GO-MF","denotations":[{"id":"T9764","span":{"begin":251,"end":264},"obj":"http://purl.obolibrary.org/obo/GO_0034062"},{"id":"T9763","span":{"begin":251,"end":264},"obj":"http://purl.obolibrary.org/obo/GO_0003968"},{"id":"T9762","span":{"begin":251,"end":264},"obj":"http://purl.obolibrary.org/obo/GO_0003899"},{"id":"T9761","span":{"begin":117,"end":119},"obj":"http://purl.obolibrary.org/obo/GO_0033968"}],"text":"Total cellular RNA was extracted from PB CD4+ T cells using an RNA isolation kit (RNeasy Mini kit; Qiagen, Valencia, CA, USA), according to the manufacturer's instructions. cDNA was synthesized from total RNA with Molony murine leukemia virus reverse transcriptase (US Biochemical, Cleveland, OH, USA) and oligo-(dT)15 primers (Promega, Madison, WI, USA). Real-time PCR was performed with the LightCycler Instrument (Roche Diagnostics, Penzberg, Germany) in glass capillaries. The reaction mix containing Taq DNA polymerase and DNA double-strand-specific SYBR Green I dye (Lightcycler FastStart DNA Master SYBR Green I; Roche Diagnostics) and specific primers were added to cDNA dilutions."}

sentences

{"project":"sentences","denotations":[{"id":"T9726","span":{"begin":477,"end":689},"obj":"Sentence"},{"id":"T9725","span":{"begin":356,"end":476},"obj":"Sentence"},{"id":"T9724","span":{"begin":0,"end":355},"obj":"Sentence"},{"id":"T70","span":{"begin":0,"end":355},"obj":"Sentence"},{"id":"T71","span":{"begin":356,"end":476},"obj":"Sentence"},{"id":"T72","span":{"begin":477,"end":689},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Total cellular RNA was extracted from PB CD4+ T cells using an RNA isolation kit (RNeasy Mini kit; Qiagen, Valencia, CA, USA), according to the manufacturer's instructions. cDNA was synthesized from total RNA with Molony murine leukemia virus reverse transcriptase (US Biochemical, Cleveland, OH, USA) and oligo-(dT)15 primers (Promega, Madison, WI, USA). Real-time PCR was performed with the LightCycler Instrument (Roche Diagnostics, Penzberg, Germany) in glass capillaries. The reaction mix containing Taq DNA polymerase and DNA double-strand-specific SYBR Green I dye (Lightcycler FastStart DNA Master SYBR Green I; Roche Diagnostics) and specific primers were added to cDNA dilutions."}

simple1

{"project":"simple1","denotations":[{"id":"T9799","span":{"begin":505,"end":508},"obj":"Protein"},{"id":"T9798","span":{"begin":41,"end":44},"obj":"Protein"}],"text":"Total cellular RNA was extracted from PB CD4+ T cells using an RNA isolation kit (RNeasy Mini kit; Qiagen, Valencia, CA, USA), according to the manufacturer's instructions. cDNA was synthesized from total RNA with Molony murine leukemia virus reverse transcriptase (US Biochemical, Cleveland, OH, USA) and oligo-(dT)15 primers (Promega, Madison, WI, USA). Real-time PCR was performed with the LightCycler Instrument (Roche Diagnostics, Penzberg, Germany) in glass capillaries. The reaction mix containing Taq DNA polymerase and DNA double-strand-specific SYBR Green I dye (Lightcycler FastStart DNA Master SYBR Green I; Roche Diagnostics) and specific primers were added to cDNA dilutions."}

BioNLP16_DUT

{"project":"BioNLP16_DUT","denotations":[{"id":"T10537","span":{"begin":505,"end":508},"obj":"Protein"},{"id":"T10536","span":{"begin":41,"end":44},"obj":"Protein"}],"text":"Total cellular RNA was extracted from PB CD4+ T cells using an RNA isolation kit (RNeasy Mini kit; Qiagen, Valencia, CA, USA), according to the manufacturer's instructions. cDNA was synthesized from total RNA with Molony murine leukemia virus reverse transcriptase (US Biochemical, Cleveland, OH, USA) and oligo-(dT)15 primers (Promega, Madison, WI, USA). Real-time PCR was performed with the LightCycler Instrument (Roche Diagnostics, Penzberg, Germany) in glass capillaries. The reaction mix containing Taq DNA polymerase and DNA double-strand-specific SYBR Green I dye (Lightcycler FastStart DNA Master SYBR Green I; Roche Diagnostics) and specific primers were added to cDNA dilutions."}

BioNLP16_Messiy

{"project":"BioNLP16_Messiy","denotations":[{"id":"T10114","span":{"begin":505,"end":508},"obj":"Protein"},{"id":"T10113","span":{"begin":41,"end":44},"obj":"Protein"}],"text":"Total cellular RNA was extracted from PB CD4+ T cells using an RNA isolation kit (RNeasy Mini kit; Qiagen, Valencia, CA, USA), according to the manufacturer's instructions. cDNA was synthesized from total RNA with Molony murine leukemia virus reverse transcriptase (US Biochemical, Cleveland, OH, USA) and oligo-(dT)15 primers (Promega, Madison, WI, USA). Real-time PCR was performed with the LightCycler Instrument (Roche Diagnostics, Penzberg, Germany) in glass capillaries. The reaction mix containing Taq DNA polymerase and DNA double-strand-specific SYBR Green I dye (Lightcycler FastStart DNA Master SYBR Green I; Roche Diagnostics) and specific primers were added to cDNA dilutions."}

DLUT931

{"project":"DLUT931","denotations":[{"id":"T10470","span":{"begin":505,"end":508},"obj":"Protein"},{"id":"T10469","span":{"begin":41,"end":44},"obj":"Protein"}],"text":"Total cellular RNA was extracted from PB CD4+ T cells using an RNA isolation kit (RNeasy Mini kit; Qiagen, Valencia, CA, USA), according to the manufacturer's instructions. cDNA was synthesized from total RNA with Molony murine leukemia virus reverse transcriptase (US Biochemical, Cleveland, OH, USA) and oligo-(dT)15 primers (Promega, Madison, WI, USA). Real-time PCR was performed with the LightCycler Instrument (Roche Diagnostics, Penzberg, Germany) in glass capillaries. The reaction mix containing Taq DNA polymerase and DNA double-strand-specific SYBR Green I dye (Lightcycler FastStart DNA Master SYBR Green I; Roche Diagnostics) and specific primers were added to cDNA dilutions."}

bionlp-st-ge-2016-test-ihmc

{"project":"bionlp-st-ge-2016-test-ihmc","denotations":[{"id":"T10533","span":{"begin":312,"end":318},"obj":"Entity"},{"id":"T10532","span":{"begin":585,"end":594},"obj":"Entity"},{"id":"T10531","span":{"begin":505,"end":508},"obj":"Protein"},{"id":"T10530","span":{"begin":505,"end":523},"obj":"Protein"},{"id":"T10529","span":{"begin":199,"end":208},"obj":"Protein"},{"id":"T10528","span":{"begin":528,"end":531},"obj":"Entity"},{"id":"T10527","span":{"begin":595,"end":618},"obj":"Entity"},{"id":"T10524","span":{"begin":595,"end":618},"obj":"Entity"},{"id":"T10520","span":{"begin":63,"end":66},"obj":"Protein"},{"id":"T10519","span":{"begin":356,"end":369},"obj":"Protein"},{"id":"T10514","span":{"begin":38,"end":80},"obj":"Entity"},{"id":"T10511","span":{"begin":528,"end":567},"obj":"Entity"},{"id":"T10510","span":{"begin":214,"end":264},"obj":"Protein"},{"id":"T10509","span":{"begin":38,"end":40},"obj":"Protein"}],"text":"Total cellular RNA was extracted from PB CD4+ T cells using an RNA isolation kit (RNeasy Mini kit; Qiagen, Valencia, CA, USA), according to the manufacturer's instructions. cDNA was synthesized from total RNA with Molony murine leukemia virus reverse transcriptase (US Biochemical, Cleveland, OH, USA) and oligo-(dT)15 primers (Promega, Madison, WI, USA). Real-time PCR was performed with the LightCycler Instrument (Roche Diagnostics, Penzberg, Germany) in glass capillaries. The reaction mix containing Taq DNA polymerase and DNA double-strand-specific SYBR Green I dye (Lightcycler FastStart DNA Master SYBR Green I; Roche Diagnostics) and specific primers were added to cDNA dilutions."}

bionlp-st-ge-2016-spacy-parsed

bionlp-st-ge-2016-test-tees

{"project":"bionlp-st-ge-2016-test-tees","denotations":[{"id":"T9778","span":{"begin":505,"end":523},"obj":"Protein"},{"id":"T9777","span":{"begin":243,"end":264},"obj":"Protein"}],"text":"Total cellular RNA was extracted from PB CD4+ T cells using an RNA isolation kit (RNeasy Mini kit; Qiagen, Valencia, CA, USA), according to the manufacturer's instructions. cDNA was synthesized from total RNA with Molony murine leukemia virus reverse transcriptase (US Biochemical, Cleveland, OH, USA) and oligo-(dT)15 primers (Promega, Madison, WI, USA). Real-time PCR was performed with the LightCycler Instrument (Roche Diagnostics, Penzberg, Germany) in glass capillaries. The reaction mix containing Taq DNA polymerase and DNA double-strand-specific SYBR Green I dye (Lightcycler FastStart DNA Master SYBR Green I; Roche Diagnostics) and specific primers were added to cDNA dilutions."}

test3

{"project":"test3","denotations":[{"id":"T9343","span":{"begin":505,"end":508},"obj":"Protein"},{"id":"T9342","span":{"begin":41,"end":44},"obj":"Protein"},{"id":"T9332","span":{"begin":505,"end":508},"obj":"Protein"},{"id":"T9331","span":{"begin":41,"end":44},"obj":"Protein"}],"text":"Total cellular RNA was extracted from PB CD4+ T cells using an RNA isolation kit (RNeasy Mini kit; Qiagen, Valencia, CA, USA), according to the manufacturer's instructions. cDNA was synthesized from total RNA with Molony murine leukemia virus reverse transcriptase (US Biochemical, Cleveland, OH, USA) and oligo-(dT)15 primers (Promega, Madison, WI, USA). Real-time PCR was performed with the LightCycler Instrument (Roche Diagnostics, Penzberg, Germany) in glass capillaries. The reaction mix containing Taq DNA polymerase and DNA double-strand-specific SYBR Green I dye (Lightcycler FastStart DNA Master SYBR Green I; Roche Diagnostics) and specific primers were added to cDNA dilutions."}

testone

{"project":"testone","denotations":[{"id":"T9307","span":{"begin":505,"end":508},"obj":"Protein"},{"id":"T9306","span":{"begin":41,"end":44},"obj":"Protein"}],"text":"Total cellular RNA was extracted from PB CD4+ T cells using an RNA isolation kit (RNeasy Mini kit; Qiagen, Valencia, CA, USA), according to the manufacturer's instructions. cDNA was synthesized from total RNA with Molony murine leukemia virus reverse transcriptase (US Biochemical, Cleveland, OH, USA) and oligo-(dT)15 primers (Promega, Madison, WI, USA). Real-time PCR was performed with the LightCycler Instrument (Roche Diagnostics, Penzberg, Germany) in glass capillaries. The reaction mix containing Taq DNA polymerase and DNA double-strand-specific SYBR Green I dye (Lightcycler FastStart DNA Master SYBR Green I; Roche Diagnostics) and specific primers were added to cDNA dilutions."}

PMC:1064873 / 11394-12083 JSONTXT

Annnotations TAB JSON ListView MergeView

PMC:1064873 / 11394-12083 JSON TXT