CORD-19:bd51ebd1d7d5c9161290c1a89d4e3fb7434db6b5 / 16943-17178 JSONTXT

{"project":"CORD-19-Sentences","denotations":[{"id":"TextSentencer_T99","span":{"begin":0,"end":235},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"The column was washed with 5 column volumes of binding buffer (50mM HEPES, 200mM sodium chloride, 10 mM beta-mercaptoethanol at pH 7.0) and refolded protein was eluted in binding buffer supplemented with 250 mM imidazole and 1 mM EDTA."}

{"project":"CORD-19_Custom_license_subset","denotations":[{"id":"T99","span":{"begin":0,"end":235},"obj":"Sentence"}],"text":"The column was washed with 5 column volumes of binding buffer (50mM HEPES, 200mM sodium chloride, 10 mM beta-mercaptoethanol at pH 7.0) and refolded protein was eluted in binding buffer supplemented with 250 mM imidazole and 1 mM EDTA."}