CORD-19:bd51ebd1d7d5c9161290c1a89d4e3fb7434db6b5 / 16943-17178 JSON TXT

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Id	Subject	Object	Predicate	Lexical cue
TextSentencer_T99	0-235	Sentence	denotes	The column was washed with 5 column volumes of binding buffer (50mM HEPES, 200mM sodium chloride, 10 mM beta-mercaptoethanol at pH 7.0) and refolded protein was eluted in binding buffer supplemented with 250 mM imidazole and 1 mM EDTA.

Id	Subject	Object	Predicate	Lexical cue
T99	0-235	Sentence	denotes	The column was washed with 5 column volumes of binding buffer (50mM HEPES, 200mM sodium chloride, 10 mM beta-mercaptoethanol at pH 7.0) and refolded protein was eluted in binding buffer supplemented with 250 mM imidazole and 1 mM EDTA.