CORD-19:824aab33809eed4758f574ccb069df25b6da76b4 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/CORD-19/sourceid/824aab33809eed4758f574ccb069df25b6da76b4","sourcedb":"CORD-19","sourceid":"824aab33809eed4758f574ccb069df25b6da76b4","text":"Pulmonary Complications of Hematopoietic Stem Cell Transplantation\n\nAbstract\npublicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.\nPulmonary complications after HSCT are common, with an incidence of 40% to 60% and with up to one third of recipients Section 15 SPECIAL CLINICAL CONDITIONS\n\nHematopoietic stem cell transplantation (HSCT) refers to the transplantation of stem cells from bone marrow, growth factorstimulated peripheral blood, and umbilical blood for the treatment of malignant and nonmalignant hematologic, autoimmune, and genetic diseases. Transplant recipients are at risk of serious complications as a result of pretransplant cytoreductive conditioning regimens, immunologic sequelae from engraftment of allogeneic lymphoid cells (which mediate graft-versus-host responses), the patient's immunosuppressed status, and infections secondary to immunosuppression. Autopsy studies show that pulmonary complications are responsible for more than 70% of deaths in HSCT recipients.\nHematopoietic stem cell transplantation is classified as autologous, allogeneic, or syngeneic. In autologous HSCT, patients receive their own hematopoietic stem cells. In allogeneic HSCT, patients receive hematopoietic stem cells from a nonidentical sibling or unrelated matched donor. In syngeneic HSCT, patients receive hematopoietic stem cells from a genetically identical twin. The source of the donor hematopoietic stem cells can be bone marrow, peripheral blood, or umbilical cord blood. Because autologous and syngeneic transplants involve stem cells that are immunologically identical to the recipient, reactions between graft and host are avoided. In allogeneic transplants, mismatch between donor and recipient human leukocyte antigens (HLAs) mediate graft-versus-host disease (GVHD), which may lead to allograft rejection.\nFactors that determine the type of transplant to be performed include the nature and stage of the underlying disease, the availability of a suitable donor, and the medical condition of the recipient. The advantages of allogeneic transplantation over autologous transplantation include a higher likelihood that the stem cell product is free of tumor contamination and graftversus-host activity.\nA survey from several countries found that the most common indications for HSCT are lymphoproliferative disorders (55%), leukemias (34%), solid tumors (6%), and nonmalignant disorders (5%). The lymphoproliferative disorders included plasma cell disorder, Hodgkin disease, and non-Hodgkin lymphoma. Autologous HSCT was slightly more common than allogeneic HSCT. For allogeneic HSCT, the most frequent malignant disease was acute myeloid leukemia (33%) and the most common nonmalignant disease, bone marrow failure syndrome (6%). For autologous HSCT, the most frequent indication was for a plasma cell disorder (41%); the most common nonmalignant indications included bone marrow failure, hemoglobinopathies, immune deficiencies, inherited diseases of metabolism, and autoimmune disorders.\nBefore undergoing HSCT, patients receive a conditioning regimen with the goals of reducing the tumor burden (with malignancy), ablating the bone marrow, and suppressing the recipient's immune system, thereby allowing engraftment of stem cells. The three regimen types are myeloablative conditioning, reduced-intensity conditioning, and nonmyeloablative conditioning. This classification is based on the duration of cytopenia and the requirement for stem cell support. Myeloablative conditioning causes irreversible cytopenia, for which stem cell support is always necessary, whereas nonmyeloablative conditioning causes minimal cytopenia, for which stem cell support may not be needed. Reduced-intensity conditioning causes cytopenia of variable duration, and stem cell support should be given.\nConventional myeloablative conditioning regimens include cyclophosphamide and total-body irradiation (TBI) or busulfan. The more recent nonmyeloablative or reduced-intensity conditioning regimens have used fludarabine and reduced-dose alkylating agents or TBI. Although these less intensive regimens do not provide a strong cytoreductive effect, they allow engraftment of the donor stem cells with a subsequent potentially beneficial graft-versus-malignancy effect. The nonmyeloablative or reduced-intensity conditioning regimens are associated with reduced transplant-associated morbidity and lower incidence of pulmonary complications after transplantation.\nProphylaxis after allogeneic transplant to prevent GVHD usually involves methotrexate, cyclosporin, corticosteroids, or in vitro T cell depletion of the graft before infusion. pulmonary complications. One study showed that elevated pretransplant TGF-β levels in patients with breast cancer undergoing autologous HSCT were associated with increased posttransplant risk of pulmonary toxicity and hepatic venoocclusive disease.\nRecipients of allogeneic transplantation have more infection complications than recipients of autografts, not only because of chronic immunosuppression, but also because GVHD itself causes an immunodeficient state by affecting the mucosal surfaces, the reticuloendothelial system, and bone marrow. These factors predispose allogeneic recipients to fatal viral pneumonias, multidrug-resistant bacteria, and invasive fungi. Similarly, bronchiolitis obliterans is almost exclusively seen after allogeneic HSCT.\nSome patients may be genetically predisposed to develop toxicity from the HSCT-related treatments. Indeed, recent studies requiring intensive care after transplantation. Respiratory failure is the most common cause of critical illness, and pneumonia is the leading infectious cause of death after HSCT (Figure 77-1) . Pulmonary complications can occur early or late in the posttransplant course, can have infectious and noninfectious etiologies, and can present with assorted radiographic findings. The pulmonary complications of HSCT also vary depending on the indication for, type of, and preparative regimen preceding HSCT.\nCellular interactions between graft and host cells are essentially eliminated with autologous transplantation, obviating the need for immunosuppression to prevent or treat GVHD. As such, autologous transplantation is associated with lower incidence of infection, particularly viral pneumonias or cytomegalovirus (CMV) pneumonitis, invasive fungal disease, and other opportunistic infections (e.g., toxoplasmosis), as well as late airflow obstruction defects.\nRelapse status at transplant and donor-recipient HLA mismatching or nonidentity are risk factors for pulmonary complications and mortality after HSCT (Box 77-1). Active phase of malignancy, age over 21 years, and receipt of HLAnonidentical donor marrow are risk factors for respiratory failure after HSCT.\nAbnormalities in pretransplant pulmonary function tests (PFTs) may be predictive of subsequent risk of pulmonary complications and mortality. Reduced diffusing capacity and increased alveolar-arterial oxygen gradient are independent risk factors for interstitial pneumonitis and also independently associated with increased early mortality after HSCT.\nPatients with elevated levels of transforming growth factor beta (TGF-β) in plasma, TGF-α in bronchoalveolar lavage (BAL) fluid, and granulocyte-macrophage colony-stimulating factor (GM-CSF) in BAL fluid seem to be at increased risk for \nThe overall risk of pulmonary infection in patients receiving HSCT depends on multiple risk factors, including chemotherapy and radiation-induced neutropenia, lung injury induced by the conditioning regimen, rejection in the form of GVHD, local disruption of host defenses, and intensity of pathogen exposure. In addition, HSCT recipients need to develop a functional immune system from donor-derived cells. Although the production of red blood cells, platelets, and granulocytes occurs soon after HSCT, production of lymphocytes, especially T cells, is considerably delayed. In the first 2 years after transplant, serious infections occur in 50% of otherwise uncomplicated transplants from histocompatible sibling donors and in 80% to 90% of matched unrelated donors or histocompatible recipients with GVHD. Supportive care in the posttransplant period has changed the microbiology of pneumonia. Prophylactic administration of trimethoprim-sulfamethoxazole (TMP-SMX), antivirals, antifungals, and fluoroquinolones has decreased the incidence of Pneumocystis jirovecii, CMV, herpes simplex, and Candida albicans infections (Box 77-2). Resistant gram-negative and grampositive bacteria, viruses, and other fungi remain important pathogens.\nBacterial pneumonia is a major cause of morbidity and mortality in patients receiving HSCT. The first month after transplant is notable for pneumonias caused by usual pathogens. Bacterial pneumonia is frequently caused by Staphylococcus and Streptococcus spp. (24% and 13%, respectively, in one series) and gram-negative organisms, including Pseudomonas, Klebsiella, Escherichia, Stenotrophomonas, Legionella, Acinetobacter, Serratia, Proteus, Enterobacter, and Citrobacter spp. Other organisms include Enterococcus and rare anaerobes such as Bacteroides and Fusobacterium spp. Community pathogens emerge after the immediate posttransplant period. Haemophilus influenzae is the most common isolate, followed by Streptococcus pneumoniae and Legionella spp.\nFungal disease should be considered in patients with persistent focal radiographic abnormalities that do not respond to empirical antibiotic therapy and in those with nodular opacities on chest imaging, prolonged neutropenia, corticosteroid use, or a history of prior fungal infections.\nsuggest that certain genetic polymorphisms appear to play a role in the pathogenesis of diverse post-HSCT pulmonary complications. For example, having a polymorphism in the gene for angiotensin-converting enzyme (ACE) increases the likelihood of developing noninfectious pulmonary dysfunction after allogeneic HSCT. Polymorphisms in genes that code for proteins involved in stress-induced oxidant and antioxidant balance may be associated with acute lung injury after HSCT. In some patients, genetic polymorphisms may also be the basis for an increased susceptibility to infection after HSCT. For example, polymorphisms in the mannose-binding lectin gene in both the donor and the recipient are associated with increased risk of major infection.\nAlthough still not routinely used in clinical practice, genetic screening may lead to a more refined prediction of risk for pulmonary complications in future patients undergoing HSCT.\nSpecific pulmonary complications associated with HSCT tend to occur in a relatively well-defined timeline. The timing and intensity of cytoreductive therapies and the resulting pattern of immune reconstitution influence the duration of these intervals.\nThe preengraftment phase (0-30 days after transplant) is characterized by prolonged neutropenia and breaks in the mucocutaneous barriers. Accordingly, infectious complications are expected and primarily caused by bacterial and fungal infections. However, most pulmonary complications during this phase are noninfectious and related to regimen-related toxicities.\nThe early postengraftment period spans days 30 to 100 after transplant and is characterized by persistent impairment in cellular and humoral immunity, in part determined by exogenous immunosuppression, GVHD, deficiency of immunoglobulins, and a loss of protective alveolar macrophages. During this period, neutropenia usually resolves, decreasing the risk of bacterial and fungal infections. The epidemiology of infectious etiologies thus changes to involve predominantly viral infections, especially CMV. With the routine use of antivirals, however, the incidence of posttransplant CMV pneumonia has decreased substantially. Noninfectious etiologies during this time include engraftment syndrome and delayed pulmonary toxicity syndrome.\nThe late postengraftment period begins at day 100 after transplant. During this time, immune recovery and function are variable and depend on the type of HSCT. Autologous recipients recover more rapidly than allogeneic recipients. T cell responses to alloantigens return to normal, but immunoglobulin levels frequently remain depressed. Viral pathogens cause infections because of poor lymphocyte function, whereas inadequate cellular immunity results in bacterial and fungal pathogens. Noninfectious etiologies are primarily responsible for the pulmonary complications seen during this time, including chronic GVHD, drug-related pulmonary toxicity, bronchiolitis obliterans, restrictive or fibrotic lung disease, and cryptogenic organizing pneumonia (formerly BOOP). presentation that includes fever, nonproductive cough, and hypoxia. Chest imaging demonstrates various abnormalities, most frequently bilateral interstitial opacities. A definitive diagnosis relies on demonstrating viral inclusion bodies in lung tissue (which can be difficult on transbronchial biopsy specimens) or detection of virus in BAL fluid by shell vial assay, polymerase chain reaction (PCR), or viral culture (in patients with a compatible clinical presentation). Mortality from CMV pneumonia in posttransplant patients exceeded 90% until the advent of combination therapy with ganciclovir and intravenous immune globulin (IVIG), which improved survival rates to as high as 70%. The use of prophylaxis has not only reduced the incidence but has also delayed the onset of disease, with more CMV infections now occurring 100 days after transplant.\nProphylactic regimens have taken two approaches: (1) universal prophylaxis to all high-risk patients for a defined period after engraftment and (2) preemptive treatment of patients only after detection of subclinical viremia by PCR assay. Both strategies reduce the risk of early CMV disease and are accepted practice guidelines. Seronegative patients who underwent allogeneic transplant from seropositive donors are at highest risk for CMV pneumonia.\nThe infection pattern of respiratory viruses in HSCT recipients is similar to that in the general population; however, HSCT recipients are more likely to develop lower respiratory tract infection, with mortality of 50% to 70%. Respiratory viruses classically recognized as causing disease in both immunocompetent and immunocompromised individuals include respiratory syncytial virus (RSV), parainfluenza, and influenza. New detection techniques have led to increasing identification of other respiratory viruses, such as human corona virus, human metapneumovirus, human bocavirus, and human rhinovirus.\nIn a large prospective study, the 2-year incidence of a first episode of a viral respiratory infection was 29% in recipients of allogeneic HSCT and 15% in recipients of autologous HSCT. The most commonly isolated respiratory viruses were influenza A/B, human RSV, and human metapneumovirus.\nRespiratory syncytial virus usually causes an upper respiratory infection, which may progress to fatal pneumonia in the immunocompromised patient. Late airflow obstruction has also been associated with RSV infection. Untreated, RSV pneumonia has a poor prognosis, with mortality approaching 80%. Treatment includes aerosolized ribavirin; other agents may be added, although a beneficial effect of concomitant therapy is not well established. Concomitant therapy includes IVIG, RSVspecific immunoglobulin, or palivizumab. Early initiation of treatment appears to be fundamental for the success of antiviral therapy. The role of preemptive therapy in the presence of isolated upper respiratory tract RSV infection is not well defined.\nAdenovirus is an uncommon cause of pneumonia and can be isolated in 3% to 5% of patients after HSCT. The incidence is higher in children. It affects the upper and lower respiratory tracts, as well as the gastrointestinal and genitourinary systems. Infection usually develops within the first 3 months of transplantation, with a presentation that might include pharyngitis, tracheitis, bronchitis, pneumonitis, enteritis, hemorrhagic cystitis, or disseminated disease. Mortality with pulmonary involvement may exceed 50%. Cidofovir is used for preemptive (positive viremia despite absence of symptoms) and therapeutic treatment. Some centers have successfully used adenovirusspecific cytotoxic T cells for treatment.\nInvasive aspergillosis is the leading cause of infectious death in HSCT patients, with a mortality of 70% to 90% in allogeneic recipients, despite treatment. The incidence of invasive aspergillosis in allogeneic recipients is 10% to 15%, with a bimodal distribution of cases. During the early preengraftment period that is characterized by profound neutropenia, both allogeneic and autologous recipients are at increased risk for invasive aspergillosis. Allogeneic patients experience a second period of vulnerability, however, during the postengraftment phase, coincident with the development of chronic GVHD, because of the need for augmented immunosuppression. The vulnerability to invasive aspergillosis is higher during the second period than during neutropenia.\nMost cases of invasive aspergillosis are limited to the lungs, but sinus and central nervous system involvement can also be seen. Common presenting symptoms include cough and dyspnea, with fever absent in up to two thirds of patients. Concomitant pleuritic chest pain and hemoptysis are clues. Radiographic findings include single or multiple nodules, cavities, and consolidation. The infiltrates are usually focal. The air crescent sign describes a central nodule partially or fully surrounded by air, indicating a sequestrum of necrotic lung tissue that has separated from the surrounding parenchyma. The halo sign on computed tomography (CT) describes a rim of low attenuation representing edema or hemorrhage that surrounds a pulmonary nodule and is present in more than 90% of patients with neutropenia with invasive pulmonary aspergillosis. The diagnosis of invasive aspergillosis is confirmed by demonstration of fungal elements in diseased tissue and a positive culture result of a specimen from a normally sterile site. In clinical practice, however, biopsy is not usually feasible because of thrombocytopenia or coagulation deficits, and a presumptive diagnosis is often substantiated by high-resolution CT (HRCT) of chest, culture results, or indirect diagnostic tests, such as detection of galactomannan antigen in body fluids.\nA randomized clinical trial comparing voriconazole with amphotericin B found that voriconazole led to better survival and improved responses of initial therapy. Therefore, voriconazole is recommended as the primary treatment for invasive aspergillosis. The voriconazole loading dosage is 6 mg/kg intravenously (IV) every 12 hours for 1 day, followed by 4 mg/kg IV every 12 hours. Although not the first choice, liposomal amphotericin B may be used as an alternative primary therapy. Posaconazole, 200 mg every 8 hours, is the first-choice agent for prophylaxis against invasive aspergillosis in high-risk patients, including HSCT recipients with GVHD.\nCandida, Cryptococcus, and Zygomycetes (including Mucor and Rhizopus) are also important pathogens. The prevalence of invasive zygomycotic infection is 2% and bears a similar clinical course to that of Aspergillus. Zygomycetes are angioinvasive, leading to thrombosis, pulmonary infarction, and hemorrhage, with radiographs showing cavitation and halo sign. Mortality rates are high (60%-80%) despite treatment with amphotericin B and surgical resection.\nThe endemic fungi are encountered less frequently. Histoplasma and Coccidioides usually occur as reactivation of latent infection. Blastomyces usually represents primary disease. Other emerging fungal pathogens reported to cause pulmonary infections include Fusarium and Scedosporium spp.\nViral infections, particularly CMV pneumonia, are an important cause of morbidity and mortality in the postengraftment period. Cytomegalovirus pneumonia has a nonspecific clinical The insight obtained from animal models has stimulated the classification of the entities under the term idiopathic pneumonia syndrome according to the primary anatomic site of cellular damage: interstitial tissue, vascular endothelium, or airway epithelium (see Fig. 77-1) . For example, in interstitial type of injury, there are increased numbers of cytotoxic T lymphocytes and cells expressing B7 family costimulatory ligands in the alveolar and interstitial spaces. In vascular endothelium type of injury, there are injured endothelial cells, and in airway epithelium type of injury, injured type II alveolar epithelial cells. This classification reflects complex interactive mechanisms with a pathogenic role attributed to several factors, including increased soluble inflammatory mediators (e.g., tumor necrosis factor alpha), increased host flora-derived lipopolysaccharide, enhanced oxidative stress, depletion of pulmonary surfactant, donor-derived T cell effectors, host antigen-presenting cells, donor accessory cells, and leukocyte recruitment to the lung.\nTreatment of the HSCT patient with IPS includes supportive care and, more recently, the combination of corticosteroids. The use of subcutaneous etanercept has been associated with a high clinical response rate in a recent series.\nIn the immediate posttransplant period, pulmonary toxicity from prior chemo/radiotherapy or the pretransplant conditioning regimen may manifest as fever, dyspnea, cough, hypoxemia, and patchy or diffuse mixed interstitial and alveolar infiltrates on chest radiography. No prospective studies document the efficacy of steroids in these patients, but in clinical practice, prednisone (1-2 mg/kg/day) is used to treat lung toxicity once infection has been excluded.\nAcute pulmonary edema is a complication that tends to occur early, typically in the second week after HSCT. Patients who develop pulmonary edema have fluid retention, as demonstrated by weight gain and a positive fluid balance in the first weeks after HSCT. The fluid retention correlates with left ventricular (LV) end-diastolic diameter measurements by echocardiography. These findings underline the importance of increased LV preload with subsequent elevation in LV enddiastolic pressure and increase in the capillary hydrostatic pressure in the pathophysiology of pulmonary edema after HSCT. Patients may also have underlying cardiac dysfunction secondary to previous treatment with chemotherapeutic agents and chest irradiation.\nNoncardiogenic pulmonary edema may result from conditions such as drug-induced pulmonary toxicity, blood product transfusion, and hepatic venooclusive disease.\nDiffuse alveolar damage is the histologic expression of acute lung injury and acute respiratory distress syndrome. It is therefore the end result of different disease processes, typically sepsis or drug toxicity. The histologic features vary according to the phase: exudative (first week) and organizing. In the exudative phase the histologic analysis reveals intraalveolar edema, interstitial widening, and hyaline membranes. In the organizing phase there is somewhat uniform fibrosis and prominent type 2 pneumocyte hyperplasia.\nDiffuse alveolar hemorrhage (DAH) occurs in approximately 5% of autologous and allogeneic recipients. Risk factors include Human metapneumovirus is an RNA paramyxovirus that causes infection in up to 5% of HSCT recipients. Patients usually present with upper respiratory tract symptoms but may also develop pneumonia. The role of antiviral therapy has not been defined in this infection.\nInfluenza types A and B can cause infection in both immunocompetent and immunocompromised patients, with type A being the most common. The 2009 influenza pandemic was caused by a novel H1N1 influenza A virus, a reassortment of classic swine H1N1, avian Eurasian swine H1N1, and North American human H2N2. Infection by novel H1N1 is characterized by severe disease in patients with specific risk factors, which include immunocompromised status. A cohort study from two medical centers found that HSCT recipients infected with novel H1N1 develop lower respiratory infection in 52% of the cases and have a 30-day mortality of 22%. Zanamivir and oseltamivir are neuraminidase inhibitors used to treat influenza types A and B. Amantadine and rimantadine are adamantanes, which are used only for influenza type A.\nOther viral infections include herpes simplex virus (HSV), varicella-zoster virus (VZV), and human herpesvirus (HHV) types 6 and 7. HSV was a common cause of infection in HSCT recipients but incidence has been greatly reduced by acyclovir prophylaxis. HHV-6 has been associated with the idiopathic pneumonia syndrome. Epstein-Barr virus (EBV) infections usually manifest as posttransplant lymphoproliferative disorder.\nThe incidence of Pneumocystis jirovecii pneumonia (formerly P. carinii pneumonia, PCP) has been greatly reduced by TMP-SMX prophylaxis. Prophylaxis is recommended from time of engraftment to 6 months after transplant in all allogeneic recipients and indefinitely for those on augmented immunosuppressive therapy and those with chronic GVHD. P. jirovecii pneumonia presents approximately 60 days after transplantation with cough, dyspnea, fever, and bilateral interstitial and alveolar infiltrates on chest radiography. Diagnostic yield of BAL is almost 90%. Despite TMP-SMX treatment, mortality can be as high as 89% for P. jirovecii infections occurring within the first 6 months after transplant versus 40% for late-onset infections.\nIdiopathic pneumonia syndrome (IPS) refers to a form of severe lung injury in which infectious etiologies, cardiac dysfunction, acute kidney injury, and iatrogenic fluid overload have been excluded. The syndrome encompasses several entities with varying clinical presentation. The heterogeneous clinical manifestations reflect the many lung insults underlying the pathogenesis of IPS, including toxic effects of HSCT conditioning regimens, immunologic cell-mediated injury, and inflammatory cytokines.\nThe incidence of IPS varies between 2.2% and 15% after allogeneic HSCT; the lower incidence is observed with nonmyeloablative conditioning regimens. The median time of onset for IPS is 22 days after HSCT (range, 4-106 days). There are reports of IPS following autologous HSCT, but it is predominantly seen after allogeneic HSCT. Risk factors for IPS include conventional conditioning regimens, older age, diagnosis of acute leukemia or myelodysplastic syndrome, development of severe acute GVHD, and high-dose TBI. any time after transplantation, with many patients remaining asymptomatic. The pulmonary function changes seen in ILD are usually secondary to treatment with conditioning regimens, particularly those containing carmustine.\nBronchiolitis obliterans (obliterative bronchiolitis) is a complication that follows allogeneic HSCT. Rarely, it may also occur after autologous HSCT. The incidence of bronchiolitis obliterans in different reports has ranged from 0% to 48%. Risk factors for obliterative bronchiolitis include chronic GVHD, older age, presence of obstructive disturbance before HSCT, viral infections of the respiratory tract, and busulfan-based conditioning regimens. The close association with GVHD has led some experts to claim that bronchiolitis obliterans may indeed represent the lung manifestation of GVHD.\nThe activation of donor-derived T lymphocytes plays an important role in the pathogenesis of bronchiolitis obliterans. T cells target the recipient epithelial cells in the bronchioles, leading to an inflammatory reaction. Toll-like receptor-4 signaling in donor-derived hematopoietic cells also appears to be an important factor in causing alloimmunity. Obliterative bronchiolitis tends to occur late, typically more than 100 days after HSCT. The most common symptoms are dry cough, dyspnea, and wheezing. The physical examination may reveal decreased breath sounds, wheezing, and inspiratory squeaks. Furthermore, physical examination often reveals signs of GVHD outside the lung such as skin manifestations. In a minority of patients, there are no respiratory manifestations, and the diagnosis is established by PFTs. The chest radiograph is usually normal, although patients with advanced disease may have signs of hyperinflation, bronchiectasis, and areas of scarring. The HRCT scan is more sensitive for all the previously mentioned abnormalities and valuable in showing air trapping and mosaic attenuation during expiration. PFTs show an obstructive pattern, with FEV 1 /FVC ratio less than 0.7 and a decrease in FEV 1 greater than 20% from pretransplant value.\nTreatment of the HSCT patient with bronchiolitis obliterans involves corticosteroids, such as prednisone at 1 to 1.5 mg/kg daily, and intensification of immunosuppressive therapy.\nPulmonary venoocclusive disease (PVOD) is characterized by obliterative fibrotic vasculopathy, which affects mainly the small branches of the pulmonary venous circulation. PVOD may be idiopathic or secondary to another disease. Diseases and risk factors associated with development of PVOD include exposure to chemotherapeutic agents (e.g., carmustine, mitomycin C, bleomycin), autologous and allogeneic HSCT, solidorgan transplantation, radiotherapy, and autoimmune diseases. Patients are seen several months after transplantation with progressive dyspnea on exertion and fatigue. They may also have pleural effusions with right upper quadrant tenderness and ascites. Chest radiography reveals interstitial edema. Abnormalities on HRCT scan include ground-glass opacities with centrolobular distribution, septal thickening, and mediastinal lymphadenopathy.\nThe diagnosis of PVOD is definitively established by histologic analysis of lung biopsy specimens, but lung biopsy is not indicated because of the high mortality risk. Histologic analysis reveals fibrosis of the venules and small veins in the interlobular septae of the pulmonary circulation, with resultant intraluminal thrombosis. The pulmonary arteries may display medial hypertrophy and eccentric intimal fibrosis. Right-sided cardiac high-dose chemotherapy received before transplant, irradiation, and advanced age. Rapid immune system reconstitution has also been theorized to contribute to DAH. Neutrophil influx into the lung may accentuate the injury and precipitate hemorrhage. The typical symptoms are dyspnea and hemoptysis. Cough and fever may also be present. Although highly suggestive of DAH in the appropriate clinical setting, hemoptysis is not always present. Patients usually are seen during the periengraftment period within the first month after transplantation, but up to 42% of patients with DAH are seen late, after day 30.\nDiagnostic criteria proposed to diagnose DAH include alveolar injury, evident as diffuse lung infiltrates or increased alveolararterial oxygen gradient; absence of pulmonary infection; BAL fluid with increasingly bloodier return from three separate subsegments; or hemosiderin-laden macrophages at 20% or higher. The radiographic findings of DAH are often nonspecific and include an interstitial or alveolar pattern, usually of bilateral distribution and sparing the peripheral lung areas.\nThe mainstay of treatment of the HSCT patient with DAH is systemic steroid therapy, which should be instituted promptly. A reported regimen is methylprednisolone, 125 to 250 mg every 6 hours for 5 days, followed by a slow taper over 2 to 4 weeks.\nEngraftment syndrome is a clinical entity that occurs more often after autologous HSCT but can also follow allogeneic HSCT. A characteristic feature is the development of the syndrome within 96 hours of engraftment. The etiology is unclear but may be attributed to increased cytokine release and neutrophil degranulation during engraftment. The main clinical manifestations of engraftment syndrome include fever, erythrodermal rash, and noncardiogenic pulmonary edema. Patients may also present with hepatic dysfunction, renal insufficiency, weight gain, and transient encephalopathy. Treatment includes supportive care and steroids. For mild cases, steroids may not be warranted.\nDelayed pulmonary toxicity syndrome refers to pulmonary toxicity caused by chemotherapy that occurs in patients who undergo autologous HSCT. This syndrome has been associated with regimens containing carmustine and cyclophosphamide. It most often presents between 6 weeks to 3 months after HSCT. It has distinct features that allow the differentiation from idiopathic pneumonia syndrome. For example, delayed pulmonary toxicity syndrome is characterized by high incidence, low mortality, and significant steroid responsiveness. Symptoms include dyspnea, nonproductive cough, and occasional fever. The radiographic abnormalities are nonspecific and do not always correlate with symptoms or worsening pulmonary function. The most common abnormalities on CT scan include ground-glass opacities and mixed linear-nodular opacities. PFTs show decreased diffusion capacity (DLCO), and forced expiratory volume in 1 second (FEV 1 ), forced vital capacity (FVC), and total lung capacity (TLC) may also be decreased but usually to a lesser extent. Lung biopsy shows alveolar septal thickening, interstitial fibrosis, and type 2 pneumocyte hyperplasia, all consistent with drug toxicity. Treatment consists of steroids.\nInterstitial pneumonitis or interstitial lung disease (ILD) manifests with diminished DLCO and TLC. It can occur at virtually\nThe term pulmonary cytolytic thrombi describes a vasculopathy that occurs in pediatric allogeneic HSCT recipients. It is characterized by an obliterative arteriopathy, occlusive vascular lesions, and hemorrhagic infarcts. This entity can resolve spontaneously without specific therapy. Pulmonary alveolar proteinosis has also been reported as a reversible cause of respiratory failure after allogeneic HSCT for acute leukemia.\nIn the diagnostic evaluation of the HSCT patient, information should be obtained on the duration of the pulmonary complication, the radiographic abnormalities, and individual patient factors, such as exposure to toxic drugs, a history of receiving chest radiotherapy, current and previous immunosuppressive regimens and infection prophylaxis, CMV status of donor and recipient, and history of previous opportunistic invasive fungal disease. Useful information may be obtained from PFTs, sputum examination, chest imaging, and serologic studies.\nThe initial imaging evaluation involves a chest radiograph, which can be normal in 15% of symptomatic patients with proven infiltrative lung disease. On the more sensitive chest CT, different patterns may suggest specific disease processes; for example, nodular infiltrates are typical of fungal infections. Nocardia and Cryptococcus also may present as nodular masses. Focal infiltrates usually reflect infectious processes. Noninfectious processes presenting as focal infiltrates include pulmonary emboli, acute radiation pneumonitis, and carcinoma. Diffuse infiltrates are nonspecific but often represent pulmonary edema in the preengraftment phase and infections after engraftment. Pleural effusions suggest bacterial, mycobacterial, or nocardial infections as well as noninfectious conditions such as pulmonary edema, hepatic venoocclusive disease, pulmonary infarction, and malignancy.\nPulmonary function tests should be obtained before HSCT, after transplant in symptomatic patients, and at regularly scheduled intervals in high-risk individuals. Pretransplant abnormalities in DLCO and alveolar-arterial oxygen difference are independent risk factors for interstitial pneumonitis and death. After transplant, typical PFT abnormalities include declining lung volumes, deceasing DLCO, and worsening airflow limitations.\nThe presence of galactomannan, a cell wall component released by Aspergillus spp., in serum indicates invasive aspergillosis, as detected by enzyme-linked immunosorbent assay. Using a cutoff optical density of 0.5, the test has a sensitivity of 97.4%, specificity of 90.5%, positive predictive value of 66.1%, and negative predictive value of 99.4%. A galactomannan assay for the diagnosis of invasive aspergillosis has also been evaluated in BAL fluid. Using a cutoff optical density of 0.5, the test showed sensitivity of 86% and specificity of 89%.\nAn elevated level of (1,3)-β-D-glucan in serum is typically associated with invasive aspergillosis and invasive candidiasis but is not specific for a particular fungal infection. catheterization typically reveals a precapillary pattern of pulmonary hypertension, with an increase in the mean pulmonary artery pressure and normal-range pulmonary capillary wedge pressure. Pulmonary vasodilators can exacerbate the pulmonary edema. Anecdotal reports describe PVOD patient response to high-dose corticosteroids.\nPosttransplant lymphoproliferative disorder is a term used for different B cell hyperproliferative states that may have benign or malignant behavior. It may follow both solid-organ transplantation and allogeneic HSCT. The lymphoid proliferation is characterized by T cell dysfunction, which results from the conditioning regimen and presence of Epstein-Barr virus. The T cell dysfunction leads to a proliferation of EBV-infected B lymphocytes. Incidence varies from 0.6% to 10%. Risk factors for this disorder include HLA-mismatched donor, T cell depletion of the graft, anti-T cell agents, older age of donor, splenectomy, and mismatch in CMV or EBV status between recipient and donor.\nThe time from transplantation to development of posttransplant lymphoproliferative disorder is 70 to 90 days. Patients are usually seen within the first 6 months after transplant with lymph node, liver, and spleen involvement, often with relapse with the original malignancy (especially lymphoma) or with secondary lymphomas. Lung involvement is seen in 20% of patients. Treatment consists of reduction in immunosuppressive therapy. Prophylactic administration of rituximab during HSCT has been proposed to prevent posttransplant lymphoproliferative disorder in high-risk patients.\nCryptogenic organizing pneumonia (COP), formerly bronchiolitis obliterans organizing pneumonia (BOOP), is an inflammatory condition that follows allogeneic and autologous HSCT. The onset of COP varies but usually occurs about 100 days after HSCT. It can be the residue of treated CMV pneumonitis, related to chronic GVHD, or can be idiopathic. Patients present with dry cough, dyspnea, and fever. Physical examination may reveal crackles and inspiratory squeaks. Chest HRCT findings include patchy consolidation, ground-glass attenuation, and randomly distributed nodules. Treatment includes steroid therapy, such as oral prednisone at an initial dose of 1 mg/kg daily, then tapered over 3 to 6 months.\nRecipients of HSCT have an overall rate of intensive care unit admission of 15.7%, and the most common cause for ICU admission is respiratory failure followed by shock secondary to sepsis. Independent risk factors for the requirement of assisted mechanical ventilation include older age, active malignancy at transplantation, and donor-recipient HLA mismatch. A review demonstrated that the pooled mortality in HSCT recipients requiring invasive mechanical ventilation is 86.4%. Noninvasive positive-pressure ventilation in select patients with reversible acute respiratory failure is used because observational studies show a survival benefit in HSCT recipients and patients with cancer. In critically ill HSCT recipients, it is also important to comply with the current evidence-based precepts of critical care medicine. 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