BB-norm@ldeleger:BB-norm-132046 JSON TXT

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Id	Subject	Object	Predicate	Lexical cue
T1	0-136	Title	denotes	[Chromosomal structures of Pseudomonas testosteroni. I. Isolation and characterization of the chromosomal complexes. (author's transl)].
T3	27-51	Microorganism	denotes	Pseudomonas testosteroni
T2	137-954	Paragraph	denotes	After lysis of Pseydomonas testosteroni with lysozyme and non-ionic detergents different DNA-protein complexes can be separated in 5-25% (w/v) neutral sucrose gradient. The protein to DNA ratio of these complexes varies between 0.5-4.5 to 1, whereby the faster sedimenting forms contain more protein than the slower sedimenting ones. Different initial rates of DNase digestion may indicate various degrees of DNA packing in these complexes. The chromosomal complexes of Pseudomonas testosteroni are relatively stable towards pronase. Treatment with RNase or sodium dodecylsulphate is accompanied by a dramatic increase in viscosity and decrease in relative density. It suggests that DNA in these complexes is maintained in its supercoiled form by RNA molecule(s) in a similar way as in isolated chromosome of E. coli.
T4	152-176	Microorganism	denotes	Pseydomonas testosteroni
T5	607-631	Microorganism	denotes	Pseudomonas testosteroni
T6	946-953	Microorganism	denotes	E. coli