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PMC:3317373 / 7860-9366 JSONTXT 23 Projects

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Id Subject Object Predicate Lexical cue
T3389 0-3 CD denotes 2.6
T3390 5-12 NNP denotes Western
T3391 13-17 NNP denotes Blot
T3392 18-20 TO denotes To
T3393 21-27 VB denotes obtain
T3394 28-33 JJ denotes total
T3395 34-41 NN denotes protein
T3396 42-49 NN denotes lysates
T3397 49-50 -COMMA- denotes ,
T3398 51-56 NN denotes cells
T3399 57-61 VB denotes were
T3400 62-73 VB denotes homogenized
T3401 74-76 IN denotes in
T3402 77-81 NN denotes RIPA
T3403 82-88 NN denotes buffer
T3404 89-90 -LRB- denotes (
T3405 90-91 CD denotes 1
T3406 91-92 NN denotes %
T3407 93-97 NN denotes NP40
T3408 97-98 -COMMA- denotes ,
T3409 99-102 CD denotes 0.5
T3410 102-103 NN denotes %
T3411 104-110 NN denotes sodium
T3412 111-123 NN denotes deoxycholate
T3413 123-124 -COMMA- denotes ,
T3414 125-128 CD denotes 0.1
T3415 128-129 NN denotes %
T3416 130-133 NN denotes SDS
T3417 133-134 -COMMA- denotes ,
T3418 135-136 CD denotes 1
T3419 137-139 NN denotes mM
T3420 140-144 NN denotes EDTA
T3421 144-145 -COMMA- denotes ,
T3422 146-147 CD denotes 1
T3423 148-150 NN denotes mM
T3424 151-155 NN denotes EGTA
T3425 155-156 -COMMA- denotes ,
T3426 157-158 CD denotes 1
T3427 159-161 NN denotes mM
T3428 162-168 NN denotes Na3VO4
T3429 168-169 -COMMA- denotes ,
T3430 170-172 CD denotes 20
T3431 173-175 NN denotes mM
T3432 176-179 NN denotes NaF
T3433 179-180 -COMMA- denotes ,
T3434 181-184 CD denotes 0.5
T3435 185-187 NN denotes mM
T3436 188-191 NN denotes DTT
T3437 191-192 -COMMA- denotes ,
T3438 193-194 CD denotes 1
T3439 195-197 NN denotes mM
T3440 198-202 NN denotes PMSF
T3441 202-203 -COMMA- denotes ,
T3442 204-207 CC denotes and
T3443 208-216 NN denotes protease
T3444 217-226 NN denotes inhibitor
T3445 227-235 NN denotes cocktail
T3446 236-238 IN denotes in
T3447 239-242 NNP denotes PBS
T3448 243-245 NN denotes pH
T3449 246-249 CD denotes 7.4
T3450 249-250 -RRB- denotes )
T3451 251-254 CC denotes and
T3452 255-266 VB denotes centrifuged
T3453 267-269 IN denotes at
T3454 270-276 CD denotes 12,000
T3455 277-278 NN denotes g
T3456 279-282 IN denotes for
T3457 283-285 CD denotes 15
T3458 286-289 NN denotes min
T3459 290-292 IN denotes at
T3460 293-296 NN denotes 4°C
T3461 298-305 NN denotes Protein
T3462 306-320 NN denotes concentrations
T3463 321-325 VB denotes were
T3464 326-335 VB denotes estimated
T3465 336-338 IN denotes by
T3466 339-348 NNP denotes Coomassie
T3467 349-353 NNP denotes Plus
T3468 354-361 NNP denotes Protein
T3469 362-367 NNP denotes Assay
T3470 368-375 NNP denotes Reagent
T3471 376-377 -LRB- denotes (
T3472 377-383 NNP denotes Pierce
T3473 383-384 -COMMA- denotes ,
T3474 385-387 NNP denotes IL
T3475 387-388 -COMMA- denotes ,
T3476 389-392 NNP denotes USA
T3477 392-393 -RRB- denotes )
T3478 395-400 CD denotes Fifty
T3479 401-411 NN denotes micrograms
T3480 412-414 IN denotes of
T3481 415-418 DT denotes the
T3482 419-428 VB denotes resulting
T3483 429-438 JJ denotes cytosolic
T3484 439-446 NN denotes protein
T3485 447-455 NN denotes extracts
T3486 456-460 VB denotes were
T3487 461-475 JJ denotes heat-denatured
T3488 476-478 IN denotes in
T3489 479-486 NNP denotes Laemmli
T3490 487-493 NN denotes sample
T3491 494-501 NN denotes loading
T3492 502-508 NN denotes buffer
T3493 508-509 -COMMA- denotes ,
T3494 510-519 VB denotes separated
T3495 520-522 IN denotes by
T3496 523-525 CD denotes 10
T3497 525-526 NN denotes %
T3498 527-533 NN denotes sodium
T3499 534-541 NN denotes dodecyl
T3500 542-549 NN denotes sulfate
T3501 550-564 NN denotes polyacrylamide
T3502 565-568 NN denotes gel
T3503 569-584 NN denotes electrophoresis
T3504 584-585 -COMMA- denotes ,
T3505 586-589 CC denotes and
T3506 590-604 VB denotes electroblotted
T3507 605-609 IN denotes onto
T3508 610-611 DT denotes a
T3509 612-626 NN denotes nitrocellulose
T3510 627-635 NN denotes membrane
T3511 637-640 IN denotes For
T3512 641-655 NN denotes immunoblotting
T3513 655-656 -COMMA- denotes ,
T3514 657-666 NN denotes membranes
T3515 667-671 VB denotes were
T3516 672-679 VB denotes blocked
T3517 680-684 IN denotes with
T3518 685-686 CD denotes 5
T3519 686-687 NN denotes %
T3520 688-694 JJ denotes nonfat
T3521 695-698 JJ denotes dry
T3522 699-703 NN denotes milk
T3523 704-706 IN denotes in
T3524 707-713 NN denotes saline
T3525 714-720 NN denotes buffer
T3526 721-730 RB denotes overnight
T3527 731-733 IN denotes at
T3528 734-737 NN denotes 4°C
T3529 737-738 -COMMA- denotes ,
T3530 739-742 CC denotes and
T3531 743-746 DT denotes the
T3532 747-756 VB denotes following
T3533 757-767 NN denotes antibodies
T3534 768-772 VB denotes were
T3535 773-777 VB denotes used
T3536 777-778 -COLON- denotes :
T3537 779-791 JJ denotes anti-β-actin
T3538 792-793 -LRB- denotes (
T3539 793-802 NN denotes sc-130657
T3540 802-803 -COMMA- denotes ,
T3541 804-809 NNP denotes Santa
T3542 810-814 NNP denotes Cruz
T3543 815-828 NNP denotes Biotechnology
T3544 828-829 -COMMA- denotes ,
T3545 830-832 NNP denotes CA
T3546 832-833 -COMMA- denotes ,
T3547 834-837 NNP denotes USA
T3548 837-838 -COMMA- denotes ,
T3549 839-841 CD denotes 43
T3550 842-845 NN denotes kDa
T3551 845-846 -RRB- denotes )
T3552 847-850 CC denotes and
T3553 851-862 JJ denotes anti-matrix
T3554 863-879 NN denotes metallopeptidase
T3555 880-881 CD denotes 9
T3556 882-883 -LRB- denotes (
T3557 883-887 NN denotes MMP9
T3558 887-888 -RRB- denotes )
T3559 889-890 -LRB- denotes (
T3560 890-897 NN denotes sc-6841
T3561 897-898 -COMMA- denotes ,
T3562 899-904 NNP denotes Santa
T3563 905-909 NNP denotes Cruz
T3564 910-923 NNP denotes Biotechnology
T3565 923-924 -COMMA- denotes ,
T3566 925-927 NNP denotes CA
T3567 927-928 -COMMA- denotes ,
T3568 929-932 NNP denotes USA
T3569 932-933 -COMMA- denotes ,
T3570 934-936 CD denotes 92
T3571 937-940 NN denotes kDa
T3572 940-941 -RRB- denotes )
T3573 943-947 DT denotes Each
T3574 948-955 JJ denotes primary
T3575 956-964 NN denotes antibody
T3576 965-968 VB denotes was
T3577 969-976 VB denotes diluted
T3578 977-990 RB denotes appropriately
T3579 991-993 IN denotes in
T3580 994-1002 VB denotes blocking
T3581 1003-1009 NN denotes buffer
T3582 1010-1013 CC denotes and
T3583 1014-1018 RB denotes then
T3584 1019-1024 VB denotes added
T3585 1025-1027 TO denotes to
T3586 1028-1031 DT denotes the
T3587 1032-1037 NN denotes blots
T3588 1038-1041 IN denotes for
T3589 1042-1043 CD denotes 1
T3590 1044-1045 NN denotes h
T3591 1046-1048 IN denotes at
T3592 1049-1053 NN denotes room
T3593 1054-1065 NN denotes temperature
T3594 1067-1070 DT denotes The
T3595 1071-1076 NN denotes blots
T3596 1077-1081 VB denotes were
T3597 1082-1088 VB denotes washed
T3598 1089-1094 CD denotes three
T3599 1095-1100 NN denotes times
T3600 1101-1103 IN denotes in
T3601 1104-1107 DT denotes the
T3602 1108-1115 VB denotes washing
T3603 1116-1122 NN denotes buffer
T3604 1123-1126 CC denotes and
T3605 1127-1134 VB denotes covered
T3606 1135-1139 IN denotes with
T3607 1140-1143 DT denotes the
T3608 1144-1155 JJ denotes horseradish
T3609 1156-1173 JJ denotes peroxidase-linked
T3610 1174-1183 JJ denotes secondary
T3611 1184-1192 NN denotes antibody
T3612 1193-1195 IN denotes at
T3613 1196-1197 DT denotes a
T3614 1198-1199 CD denotes 1
T3615 1200-1201 -COLON- denotes :
T3616 1202-1206 CD denotes 2000
T3617 1207-1215 NN denotes dilution
T3618 1216-1219 IN denotes for
T3619 1220-1221 CD denotes 1
T3620 1222-1223 NN denotes h
T3621 1225-1230 NN denotes Blots
T3622 1231-1235 VB denotes were
T3623 1236-1245 VB denotes incubated
T3624 1246-1250 IN denotes with
T3625 1251-1259 VB denotes enhanced
T3626 1260-1277 NN denotes chemiluminescence
T3627 1278-1279 -LRB- denotes (
T3628 1279-1282 NN denotes ECL
T3629 1282-1283 -RRB- denotes )
T3630 1284-1293 NN denotes detection
T3631 1294-1300 NN denotes system
T3632 1301-1302 -LRB- denotes (
T3633 1302-1310 NNP denotes Amersham
T3634 1311-1322 NNP denotes Biosciences
T3635 1322-1323 -COMMA- denotes ,
T3636 1324-1329 NNP denotes Bucks
T3637 1329-1330 -COMMA- denotes ,
T3638 1331-1333 NNP denotes UK
T3639 1333-1334 -RRB- denotes )
T3640 1335-1338 CC denotes and
T3641 1339-1346 VB denotes exposed
T3642 1347-1349 TO denotes to
T3643 1350-1362 JJ denotes radiographic
T3644 1363-1367 NN denotes film
T3645 1368-1369 -LRB- denotes (
T3646 1369-1373 NNP denotes Fuji
T3647 1374-1383 NNP denotes Hyperfilm
T3648 1383-1384 -COMMA- denotes ,
T3649 1385-1390 NNP denotes Tokyo
T3650 1390-1391 -COMMA- denotes ,
T3651 1392-1397 NNP denotes Japan
T3652 1397-1398 -RRB- denotes )
T3653 1400-1406 NN denotes ImageJ
T3654 1407-1410 VB denotes was
T3655 1411-1415 VB denotes used
T3656 1416-1418 TO denotes to
T3657 1419-1426 VB denotes analyze
T3658 1427-1430 DT denotes the
T3659 1431-1440 NN denotes intensity
T3660 1441-1443 IN denotes of
T3661 1444-1447 DT denotes the
T3662 1448-1453 NN denotes blots
T3663 1455-1458 DT denotes The
T3664 1459-1464 NN denotes level
T3665 1465-1467 IN denotes of
T3666 1468-1475 NN denotes β-actin
T3667 1476-1479 VB denotes was
T3668 1480-1484 VB denotes used
T3669 1485-1487 IN denotes as
T3670 1488-1496 JJ denotes internal
T3671 1497-1505 NN denotes standard
R2899 T3391 T3390 arg1Of Blot,Western
R2900 T3393 T3392 arg1Of obtain,To
R2901 T3396 T3393 arg2Of lysates,obtain
R2902 T3396 T3394 arg1Of lysates,total
R2903 T3396 T3395 arg1Of lysates,protein
R2904 T3398 T3393 arg1Of cells,obtain
R2905 T3398 T3399 arg1Of cells,were
R2906 T3398 T3400 arg2Of cells,homogenized
R2907 T3398 T3452 arg2Of cells,centrifuged
R2908 T3400 T3401 arg1Of homogenized,in
R2909 T3400 T3451 arg1Of homogenized,and
R2910 T3403 T3401 arg2Of buffer,in
R2911 T3403 T3402 arg1Of buffer,RIPA
R2912 T3403 T3404 arg1Of buffer,(
R2913 T3405 T3406 arg1Of 1,%
R2914 T3407 T3405 arg1Of NP40,1
R2915 T3407 T3408 arg1Of NP40,","
R2916 T3408 T3413 arg1Of ",",","
R2917 T3409 T3410 arg1Of 0.5,%
R2918 T3412 T3408 arg2Of deoxycholate,","
R2919 T3412 T3409 arg1Of deoxycholate,0.5
R2920 T3412 T3411 arg1Of deoxycholate,sodium
R2921 T3413 T3417 arg1Of ",",","
R2922 T3414 T3415 arg1Of 0.1,%
R2923 T3416 T3413 arg2Of SDS,","
R2924 T3416 T3414 arg1Of SDS,0.1
R2925 T3417 T3421 arg1Of ",",","
R2926 T3418 T3419 arg1Of 1,mM
R2927 T3420 T3417 arg2Of EDTA,","
R2928 T3420 T3418 arg1Of EDTA,1
R2929 T3421 T3404 arg2Of ",",(
R2930 T3421 T3425 arg1Of ",",","
R2931 T3422 T3423 arg1Of 1,mM
R2932 T3424 T3421 arg2Of EGTA,","
R2933 T3424 T3422 arg1Of EGTA,1
R2934 T3426 T3427 arg1Of 1,mM
R2935 T3428 T3426 arg1Of Na3VO4,1
R2936 T3428 T3429 arg1Of Na3VO4,","
R2937 T3429 T3433 arg1Of ",",","
R2938 T3430 T3431 arg1Of 20,mM
R2939 T3432 T3429 arg2Of NaF,","
R2940 T3432 T3430 arg1Of NaF,20
R2941 T3433 T3437 arg1Of ",",","
R2942 T3434 T3435 arg1Of 0.5,mM
R2943 T3436 T3433 arg2Of DTT,","
R2944 T3436 T3434 arg1Of DTT,0.5
R2945 T3437 T3442 arg1Of ",",and
R2946 T3438 T3439 arg1Of 1,mM
R2947 T3440 T3437 arg2Of PMSF,","
R2948 T3440 T3438 arg1Of PMSF,1
R2949 T3442 T3425 arg2Of and,","
R2950 T3442 T3441 arg1Of and,","
R2951 T3445 T3442 arg2Of cocktail,and
R2952 T3445 T3443 arg1Of cocktail,protease
R2953 T3445 T3444 arg1Of cocktail,inhibitor
R2954 T3445 T3446 arg1Of cocktail,in
R2955 T3448 T3446 arg2Of pH,in
R2956 T3448 T3447 arg1Of pH,PBS
R2957 T3448 T3449 arg1Of pH,7.4
R2958 T3450 T3404 arg3Of ),(
R2959 T3451 T3392 modOf and,To
R2960 T3451 T3397 arg1Of and,","
R2961 T3451 T3399 arg2Of and,were
R2962 T3452 T3451 arg2Of centrifuged,and
R2963 T3452 T3453 arg1Of centrifuged,at
R2964 T3452 T3456 arg1Of centrifuged,for
R2965 T3455 T3453 arg2Of g,at
R2966 T3455 T3454 arg1Of g,"12,000"
R2967 T3458 T3456 arg2Of min,for
R2968 T3458 T3457 arg1Of min,15
R2969 T3458 T3459 arg1Of min,at
R2970 T3460 T3459 arg2Of 4°C,at
R2971 T3462 T3461 arg1Of concentrations,Protein
R2972 T3462 T3463 arg1Of concentrations,were
R2973 T3462 T3464 arg2Of concentrations,estimated
R2974 T3464 T3463 arg2Of estimated,were
R2975 T3470 T3464 arg1Of Reagent,estimated
R2976 T3470 T3465 arg2Of Reagent,by
R2977 T3470 T3466 arg1Of Reagent,Coomassie
R2978 T3470 T3467 arg1Of Reagent,Plus
R2979 T3470 T3468 arg1Of Reagent,Protein
R2980 T3470 T3469 arg1Of Reagent,Assay
R2981 T3470 T3471 arg1Of Reagent,(
R2982 T3472 T3473 arg1Of Pierce,","
R2983 T3473 T3471 arg2Of ",",(
R2984 T3473 T3475 arg1Of ",",","
R2985 T3474 T3473 arg2Of IL,","
R2986 T3476 T3475 arg2Of USA,","
R2987 T3477 T3471 arg3Of ),(
R2988 T3479 T3478 arg1Of micrograms,Fifty
R2989 T3479 T3480 arg1Of micrograms,of
R2990 T3479 T3486 arg1Of micrograms,were
R2991 T3479 T3487 arg1Of micrograms,heat-denatured
R2992 T3479 T3506 arg1Of micrograms,electroblotted
R2993 T3485 T3480 arg2Of extracts,of
R2994 T3485 T3481 arg1Of extracts,the
R2995 T3485 T3482 arg1Of extracts,resulting
R2996 T3485 T3483 arg1Of extracts,cytosolic
R2997 T3485 T3484 arg1Of extracts,protein
R2998 T3486 T3488 arg1Of were,in
R2999 T3486 T3505 arg1Of were,and
R3000 T3487 T3486 arg2Of heat-denatured,were
R3001 T3492 T3488 arg2Of buffer,in
R3002 T3492 T3489 arg1Of buffer,Laemmli
R3003 T3492 T3490 arg1Of buffer,sample
R3004 T3492 T3491 arg1Of buffer,loading
R3005 T3492 T3493 arg1Of buffer,","
R3006 T3492 T3494 arg2Of buffer,separated
R3007 T3496 T3497 arg1Of 10,%
R3008 T3503 T3494 arg1Of electrophoresis,separated
R3009 T3503 T3495 arg2Of electrophoresis,by
R3010 T3503 T3496 arg1Of electrophoresis,10
R3011 T3503 T3498 arg1Of electrophoresis,sodium
R3012 T3503 T3499 arg1Of electrophoresis,dodecyl
R3013 T3503 T3500 arg1Of electrophoresis,sulfate
R3014 T3503 T3501 arg1Of electrophoresis,polyacrylamide
R3015 T3503 T3502 arg1Of electrophoresis,gel
R3016 T3505 T3504 arg1Of and,","
R3017 T3506 T3505 arg2Of electroblotted,and
R3018 T3506 T3507 arg1Of electroblotted,onto
R3019 T3510 T3507 arg2Of membrane,onto
R3020 T3510 T3508 arg1Of membrane,a
R3021 T3510 T3509 arg1Of membrane,nitrocellulose
R3022 T3512 T3511 arg2Of immunoblotting,For
R3023 T3514 T3515 arg1Of membranes,were
R3024 T3514 T3516 arg2Of membranes,blocked
R3025 T3516 T3511 arg1Of blocked,For
R3026 T3516 T3513 arg1Of blocked,","
R3027 T3516 T3515 arg2Of blocked,were
R3028 T3516 T3517 arg1Of blocked,with
R3029 T3516 T3526 arg1Of blocked,overnight
R3030 T3516 T3527 arg1Of blocked,at
R3031 T3516 T3530 arg1Of blocked,and
R3032 T3518 T3519 arg1Of 5,%
R3033 T3522 T3517 arg2Of milk,with
R3034 T3522 T3518 arg1Of milk,5
R3035 T3522 T3520 arg1Of milk,nonfat
R3036 T3522 T3521 arg1Of milk,dry
R3037 T3522 T3523 arg1Of milk,in
R3038 T3525 T3523 arg2Of buffer,in
R3039 T3525 T3524 arg1Of buffer,saline
R3040 T3528 T3527 arg2Of 4°C,at
R3041 T3530 T3529 arg1Of and,","
R3042 T3530 T3536 arg1Of and,:
R3043 T3530 T3537 arg1Of and,anti-β-actin
R3044 T3530 T3554 arg1Of and,metallopeptidase
R3045 T3533 T3531 arg1Of antibodies,the
R3046 T3533 T3532 arg1Of antibodies,following
R3047 T3533 T3534 arg1Of antibodies,were
R3048 T3533 T3535 arg2Of antibodies,used
R3049 T3535 T3530 arg2Of used,and
R3050 T3535 T3534 arg2Of used,were
R3051 T3537 T3538 arg1Of anti-β-actin,(
R3052 T3537 T3552 arg1Of anti-β-actin,and
R3053 T3539 T3540 arg1Of sc-130657,","
R3054 T3539 T3544 arg1Of sc-130657,","
R3055 T3543 T3540 arg2Of Biotechnology,","
R3056 T3543 T3541 arg1Of Biotechnology,Santa
R3057 T3543 T3542 arg1Of Biotechnology,Cruz
R3058 T3544 T3538 arg2Of ",",(
R3059 T3545 T3546 arg1Of CA,","
R3060 T3546 T3548 arg1Of ",",","
R3061 T3547 T3546 arg2Of USA,","
R3062 T3549 T3548 arg2Of 43,","
R3063 T3550 T3544 arg2Of kDa,","
R3064 T3550 T3545 arg1Of kDa,CA
R3065 T3550 T3547 arg1Of kDa,USA
R3066 T3550 T3549 arg1Of kDa,43
R3067 T3551 T3538 arg3Of ),(
R3068 T3552 T3559 arg1Of and,(
R3069 T3554 T3552 arg2Of metallopeptidase,and
R3070 T3554 T3553 arg1Of metallopeptidase,anti-matrix
R3071 T3554 T3555 arg1Of metallopeptidase,9
R3072 T3554 T3556 arg1Of metallopeptidase,(
R3073 T3557 T3556 arg2Of MMP9,(
R3074 T3558 T3556 arg3Of ),(
R3075 T3560 T3561 arg1Of sc-6841,","
R3076 T3560 T3565 arg1Of sc-6841,","
R3077 T3564 T3561 arg2Of Biotechnology,","
R3078 T3564 T3562 arg1Of Biotechnology,Santa
R3079 T3564 T3563 arg1Of Biotechnology,Cruz
R3080 T3565 T3559 arg2Of ",",(
R3081 T3566 T3567 arg1Of CA,","
R3082 T3567 T3569 arg1Of ",",","
R3083 T3568 T3567 arg2Of USA,","
R3084 T3570 T3569 arg2Of 92,","
R3085 T3571 T3565 arg2Of kDa,","
R3086 T3571 T3566 arg1Of kDa,CA
R3087 T3571 T3568 arg1Of kDa,USA
R3088 T3571 T3570 arg1Of kDa,92
R3089 T3572 T3559 arg3Of ),(
R3090 T3575 T3573 arg1Of antibody,Each
R3091 T3575 T3574 arg1Of antibody,primary
R3092 T3575 T3576 arg1Of antibody,was
R3093 T3575 T3577 arg2Of antibody,diluted
R3094 T3575 T3584 arg1Of antibody,added
R3095 T3577 T3576 arg2Of diluted,was
R3096 T3577 T3578 arg1Of diluted,appropriately
R3097 T3577 T3579 arg1Of diluted,in
R3098 T3577 T3582 arg1Of diluted,and
R3099 T3580 T3579 arg2Of blocking,in
R3100 T3581 T3580 arg2Of buffer,blocking
R3101 T3584 T3582 arg2Of added,and
R3102 T3584 T3583 arg1Of added,then
R3103 T3584 T3585 arg1Of added,to
R3104 T3584 T3588 arg1Of added,for
R3105 T3587 T3585 arg2Of blots,to
R3106 T3587 T3586 arg1Of blots,the
R3107 T3590 T3588 arg2Of h,for
R3108 T3590 T3589 arg1Of h,1
R3109 T3590 T3591 arg1Of h,at
R3110 T3593 T3591 arg2Of temperature,at
R3111 T3593 T3592 arg1Of temperature,room
R3112 T3595 T3594 arg1Of blots,The
R3113 T3595 T3596 arg1Of blots,were
R3114 T3595 T3597 arg2Of blots,washed
R3115 T3595 T3605 arg2Of blots,covered
R3116 T3597 T3599 arg1Of washed,times
R3117 T3597 T3600 arg1Of washed,in
R3118 T3597 T3604 arg1Of washed,and
R3119 T3599 T3598 arg1Of times,three
R3120 T3603 T3600 arg2Of buffer,in
R3121 T3603 T3601 arg1Of buffer,the
R3122 T3603 T3602 arg1Of buffer,washing
R3123 T3604 T3596 arg2Of and,were
R3124 T3605 T3604 arg2Of covered,and
R3125 T3605 T3606 arg1Of covered,with
R3126 T3605 T3612 arg1Of covered,at
R3127 T3611 T3606 arg2Of antibody,with
R3128 T3611 T3607 arg1Of antibody,the
R3129 T3611 T3608 arg1Of antibody,horseradish
R3130 T3611 T3609 arg1Of antibody,peroxidase-linked
R3131 T3611 T3610 arg1Of antibody,secondary
R3132 T3614 T3612 arg2Of 1,at
R3133 T3614 T3613 arg1Of 1,a
R3134 T3614 T3615 arg1Of 1,:
R3135 T3617 T3616 arg1Of dilution,2000
R3136 T3617 T3618 arg1Of dilution,for
R3137 T3620 T3618 arg2Of h,for
R3138 T3620 T3619 arg1Of h,1
R3139 T3621 T3622 arg1Of Blots,were
R3140 T3621 T3623 arg2Of Blots,incubated
R3141 T3621 T3641 arg2Of Blots,exposed
R3142 T3623 T3624 arg1Of incubated,with
R3143 T3623 T3640 arg1Of incubated,and
R3144 T3628 T3627 arg2Of ECL,(
R3145 T3629 T3627 arg3Of ),(
R3146 T3631 T3624 arg2Of system,with
R3147 T3631 T3625 arg2Of system,enhanced
R3148 T3631 T3626 arg1Of system,chemiluminescence
R3149 T3631 T3627 arg1Of system,(
R3150 T3631 T3630 arg1Of system,detection
R3151 T3631 T3632 arg1Of system,(
R3152 T3634 T3632 arg2Of Biosciences,(
R3153 T3634 T3633 arg1Of Biosciences,Amersham
R3154 T3634 T3635 arg1Of Biosciences,","
R3155 T3636 T3635 arg2Of Bucks,","
R3156 T3636 T3637 arg1Of Bucks,","
R3157 T3638 T3637 arg2Of UK,","
R3158 T3639 T3632 arg3Of ),(
R3159 T3640 T3622 arg2Of and,were
R3160 T3641 T3640 arg2Of exposed,and
R3161 T3641 T3642 arg1Of exposed,to
R3162 T3644 T3642 arg2Of film,to
R3163 T3644 T3643 arg1Of film,radiographic
R3164 T3644 T3645 arg1Of film,(
R3165 T3647 T3645 arg2Of Hyperfilm,(
R3166 T3647 T3646 arg1Of Hyperfilm,Fuji
R3167 T3647 T3648 arg1Of Hyperfilm,","
R3168 T3649 T3648 arg2Of Tokyo,","
R3169 T3649 T3650 arg1Of Tokyo,","
R3170 T3651 T3650 arg2Of Japan,","
R3171 T3652 T3645 arg3Of ),(
R3172 T3653 T3654 arg1Of ImageJ,was
R3173 T3653 T3655 arg2Of ImageJ,used
R3174 T3655 T3654 arg2Of used,was
R3175 T3657 T3655 arg3Of analyze,used
R3176 T3657 T3656 arg1Of analyze,to
R3177 T3659 T3657 arg2Of intensity,analyze
R3178 T3659 T3658 arg1Of intensity,the
R3179 T3659 T3660 arg1Of intensity,of
R3180 T3662 T3660 arg2Of blots,of
R3181 T3662 T3661 arg1Of blots,the
R3182 T3664 T3663 arg1Of level,The
R3183 T3664 T3665 arg1Of level,of
R3184 T3664 T3667 arg1Of level,was
R3185 T3664 T3668 arg2Of level,used
R3186 T3666 T3665 arg2Of β-actin,of
R3187 T3668 T3667 arg2Of used,was
R3188 T3668 T3669 arg1Of used,as
R3189 T3671 T3669 arg2Of standard,as
R3190 T3671 T3670 arg1Of standard,internal