PubMed:12914241 JSONTXT

[Deletion mutation of beta-amyloid precursor protein decreases the secretion of beta-amyloid in SH-SY5Y neuroblastoma cells]. OBJECTIVE: To explore the effect of A beta secretion lacking exons 2 and 3 of APP695 (delta APP695) on SH-SY5Y neuroblastoma cells. METHODS: APP695 cDNA and delta APP695 cDNA eukaryotic expression vectors were constructed by gene recombination. Immunoprecipitation Western blotting was used to analyse the A beta secretion and MTT assay to observe the cell viability's change after being stably transfected with the plasmids by lipofectamine method. RESULTS: Stable transfectants were selected in 0.6 g.L-1 G418. delta APP695 cDNA construct transfection decreased significantly the production of beta amyloid in the SH-SY5Y compared with APP695 cDNA construct. It did not affect the cell viability by MTT method. CONCLUSION: This result suggests that the alternative splicing of APP exons 2 and 3 modulates the secretion of A beta by influencing the processing of APP.