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Light microscope based analysis of three-dimensional structure: applications to the study of Drosophila salivary gland nuclei. II. Algorithms for model analysis.
In Drosophila melanogaster there are significant differences in the way the polytene chromosomes are arranged in different nuclei from the same salivary gland (Mathog et al., 1984). Visual inspection of the models of these nuclei was inadequate to delineate all of the features conserved in their structures. In order to bypass this limitation, models of these nuclei were constructed in a format compatible with computational manipulation (Mathog et al., 1985). By applying some simple algorithms to these models, quantitative comparisons were made which revealed otherwise cryptic features common to many nuclei. Given here are the details of several algorithms for describing and comparing the arrangement of the chromosomes within the nuclei.
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