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[Method of determination of biogenic amines in wines by high-performance liquid chromatography with fluorescence detector and ultraviolet detector].
OBJECTIVE: A pre-column derivation reversed-phase high-performance liquid chromatography method was developed for the determination of biogenic amines (tryptamine, phenylethyamine, putrescine, cadaverine, histamine, tyramine, spermidine, and spermine) in wines with fluorescecse detector (FLD) and ultraviolet detector (UVD).
METHODS: Liqiud-liqiud extraction (LLE) was used to clean up wine samples with chloroform/butanol prior to derivatization with dansyl chloride, biogenic amines were separated using gradient elution. Mobile phase A was methanol and mobile phase B was water with the flow rate 1.5 ml/min. The column used was a CAPCELL PAK C18 MG (4.6 mm I.D. x 150 mm, 5 microm) and gradient elution at constant column tempereture with 30 degrees C under fluorescence detector with Ex 350nm and Em 520nm, The method and ultraviolet detector with 254nm. 1,7-Diaminoheptane was used as the internal standard (IS).
RESULTS: was linear for the amines studied at concentration ranging from 0.05 to 25 mg/L, except for spermidine and spermine, which ranging from 0.05 to 15 mg/L. The average recoveries ranged from 79.2% to 127.5% for all amines, The RSDs were less than 10%. With this method, 5 wine samples were determined, the results were favorable.
CONCLUSION: method can be applied for determination of biogenic amines in wines.
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