SeeDev-binary@ldeleger:SeeDev-binary-16492731-1 JSONTXT

{"project":"bionlp-ost-19-SeeDev-bin-test","denotations":[{"id":"T1","span":{"begin":4,"end":21},"obj":"Protein_Family"},{"id":"T2","span":{"begin":22,"end":38},"obj":"Protein"},{"id":"T3","span":{"begin":40,"end":44},"obj":"Protein"},{"id":"T4","span":{"begin":81,"end":94},"obj":"Development_Phase"},{"id":"T5","span":{"begin":110,"end":126},"obj":"Development_Phase"},{"id":"T6","span":{"begin":156,"end":182},"obj":"Development_Phase"},{"id":"T7","span":{"begin":164,"end":170},"obj":"Tissue"},{"id":"T8","span":{"begin":186,"end":202},"obj":"Tissue"},{"id":"T9","span":{"begin":230,"end":234},"obj":"Protein"},{"id":"T10","span":{"begin":255,"end":331},"obj":"Regulatory_Network"},{"id":"T11","span":{"begin":295,"end":331},"obj":"Development_Phase"},{"id":"T12","span":{"begin":315,"end":319},"obj":"Tissue"},{"id":"T13","span":{"begin":333,"end":372},"obj":"Genotype"},{"id":"T14","span":{"begin":346,"end":350},"obj":"Protein"},{"id":"T15","span":{"begin":363,"end":372},"obj":"Tissue"},{"id":"T16","span":{"begin":402,"end":461},"obj":"RNA"},{"id":"T17","span":{"begin":445,"end":461},"obj":"Development_Phase"},{"id":"T18","span":{"begin":512,"end":532},"obj":"Regulatory_Network"},{"id":"T19-0","span":{"begin":544,"end":556},"obj":"_FRAGMENT"},{"id":"T19-1","span":{"begin":572,"end":580},"obj":"Protein_Family"},{"id":"T20","span":{"begin":544,"end":548},"obj":"Tissue"},{"id":"T21","span":{"begin":561,"end":580},"obj":"Protein_Family"},{"id":"T22","span":{"begin":619,"end":636},"obj":"RNA"},{"id":"T23","span":{"begin":619,"end":623},"obj":"Protein"},{"id":"T24","span":{"begin":680,"end":696},"obj":"Development_Phase"},{"id":"T25","span":{"begin":777,"end":796},"obj":"Promoter"},{"id":"T26","span":{"begin":797,"end":799},"obj":"Box"},{"id":"T27","span":{"begin":852,"end":883},"obj":"Protein_Family"},{"id":"T28","span":{"begin":914,"end":918},"obj":"Protein"},{"id":"T29","span":{"begin":938,"end":940},"obj":"Box"},{"id":"T30","span":{"begin":957,"end":999},"obj":"Promoter"},{"id":"T31","span":{"begin":980,"end":984},"obj":"Protein"},{"id":"T32","span":{"begin":1078,"end":1082},"obj":"Protein"},{"id":"T33","span":{"begin":1099,"end":1116},"obj":"RNA"},{"id":"T34","span":{"begin":1099,"end":1103},"obj":"Protein"},{"id":"T35","span":{"begin":1149,"end":1165},"obj":"Development_Phase"},{"id":"T36","span":{"begin":1200,"end":1205},"obj":"RNA"},{"id":"T37","span":{"begin":1200,"end":1205},"obj":"Gene"},{"id":"T38","span":{"begin":1210,"end":1215},"obj":"RNA"},{"id":"T39","span":{"begin":1210,"end":1215},"obj":"Gene"},{"id":"T40","span":{"begin":1231,"end":1255},"obj":"Tissue"},{"id":"T41","span":{"begin":1290,"end":1294},"obj":"Protein"},{"id":"T42","span":{"begin":1355,"end":1359},"obj":"Protein"},{"id":"T43","span":{"begin":1367,"end":1383},"obj":"Development_Phase"},{"id":"T44","span":{"begin":1408,"end":1429},"obj":"Development_Phase"}],"relations":[{"id":"C-T19-0","pred":"_lexicallyChainedTo","subj":"T19-1","obj":"T19-0"}],"text":"The B3 domain protein LEAFY COTYLEDON2 (LEC2) is required for several aspects of embryogenesis, including the maturation phase,\nand is sufficient to induce somatic embryo development in vegetative cells. Here, we demonstrate that LEC2 directly controls\na transcriptional program involved in the maturation phase of seed development. Induction of LEC2 activity in seedlings causes\nrapid accumulation of RNAs normally present primarily during the maturation phase. Several RNAs encode proteins with known\nroles in maturation processes, including seed-storage and lipid-body proteins. Clustering analyses identified other LEC2-induced\nRNAs not previously shown to be involved in the maturation phase. We show further that genes encoding these maturation RNAs\nall possess in their 5′ flanking regions RY motifs, DNA elements bound by other closely related B3 domain transcription factors.\nOur finding that recombinant LEC2 specifically binds RY motifs from the 5′ flanking regions of LEC2-induced genes provides\nstrong evidence that these genes represent transcriptional targets of LEC2. Although these LEC2-induced RNAs accumulate primarily\nduring the maturation phase, we show that a subset, including AGL15 and IAA30, accumulate in seeds containing zygotes. We discuss how identification of LEC2 target genes provides a potential link between\nthe roles of LEC2 in the maturation phase and in the induction of somatic embryogenesis.\n"}