SeeDev-binary@ldeleger:SeeDev-binary-16387832-1 JSONTXT

{"project":"bionlp-ost-19-SeeDev-bin-test","denotations":[{"id":"T1","span":{"begin":0,"end":29},"obj":"Development_Phase"},{"id":"T2","span":{"begin":73,"end":89},"obj":"Tissue"},{"id":"T3-0","span":{"begin":93,"end":98},"obj":"_FRAGMENT"},{"id":"T3-1","span":{"begin":108,"end":117},"obj":"Tissue"},{"id":"T4","span":{"begin":103,"end":117},"obj":"Tissue"},{"id":"T5","span":{"begin":246,"end":267},"obj":"Regulatory_Network"},{"id":"T6","span":{"begin":285,"end":308},"obj":"Genotype"},{"id":"T7","span":{"begin":298,"end":301},"obj":"Genotype"},{"id":"T8","span":{"begin":325,"end":368},"obj":"Regulatory_Network"},{"id":"T9","span":{"begin":335,"end":368},"obj":"Development_Phase"},{"id":"T10","span":{"begin":354,"end":368},"obj":"Tissue"},{"id":"T11","span":{"begin":373,"end":406},"obj":"Regulatory_Network"},{"id":"T12","span":{"begin":396,"end":406},"obj":"Tissue"},{"id":"T13","span":{"begin":452,"end":455},"obj":"Genotype"},{"id":"T14","span":{"begin":493,"end":501},"obj":"Gene"},{"id":"T15","span":{"begin":503,"end":506},"obj":"Gene"},{"id":"T16","span":{"begin":554,"end":571},"obj":"Development_Phase"},{"id":"T17","span":{"begin":576,"end":592},"obj":"Development_Phase"},{"id":"T18","span":{"begin":613,"end":620},"obj":"Gene"},{"id":"T19","span":{"begin":622,"end":625},"obj":"Gene"},{"id":"T20","span":{"begin":631,"end":639},"obj":"Gene"},{"id":"T21","span":{"begin":641,"end":645},"obj":"Gene"},{"id":"T22","span":{"begin":669,"end":690},"obj":"Regulatory_Network"},{"id":"T23","span":{"begin":698,"end":707},"obj":"Genotype"},{"id":"T24","span":{"begin":727,"end":730},"obj":"Genotype"},{"id":"T25","span":{"begin":731,"end":743},"obj":"Tissue"},{"id":"T26","span":{"begin":776,"end":793},"obj":"Development_Phase"},{"id":"T27","span":{"begin":795,"end":803},"obj":"RNA"},{"id":"T28","span":{"begin":795,"end":798},"obj":"Gene"},{"id":"T29","span":{"begin":821,"end":849},"obj":"Tissue"},{"id":"T30","span":{"begin":890,"end":897},"obj":"Gene"},{"id":"T31","span":{"begin":923,"end":926},"obj":"Gene"},{"id":"T32","span":{"begin":931,"end":952},"obj":"Regulatory_Network"},{"id":"T33","span":{"begin":960,"end":975},"obj":"Tissue"},{"id":"T34","span":{"begin":1005,"end":1046},"obj":"Development_Phase"},{"id":"T35","span":{"begin":1032,"end":1046},"obj":"Tissue"},{"id":"T36","span":{"begin":1050,"end":1053},"obj":"Genotype"},{"id":"T37","span":{"begin":1081,"end":1102},"obj":"Regulatory_Network"},{"id":"T38","span":{"begin":1120,"end":1123},"obj":"Gene"},{"id":"T40","span":{"begin":1137,"end":1158},"obj":"Regulatory_Network"},{"id":"T39","span":{"begin":1137,"end":1158},"obj":"Regulatory_Network"},{"id":"T41","span":{"begin":1176,"end":1179},"obj":"Gene"},{"id":"T42","span":{"begin":1180,"end":1184},"obj":"Gene"}],"relations":[{"id":"C-T3-0","pred":"_lexicallyChainedTo","subj":"T3-1","obj":"T3-0"}],"text":"Postembryonic organ formation in higher plants relies on the activity of stem cell niches in shoot and root meristems where\ndifferentiation of the resident cells is repressed by signals from surrounding cells. We searched for mutations affecting\nstem cell maintenance and isolated the semidominant l28 mutant, which displays premature termination of the shoot meristem and differentiation of the stem cells. Allele competition\nexperiments suggest that l28 is a dominant-negative allele of the APETALA2 (AP2) gene, which previously has been implicated in floral patterning and seed development. Expression of both WUSCHEL (WUS) and CLAVATA3 (CLV3) genes, which regulate stem cell maintenance in the wild type, were disrupted in l28 shoot apices from early stages on. Unlike in floral patterning, AP2 mRNA is active in the center of the shoot meristem and acts via a mechanism independent of AGAMOUS, which is a repressor of WUS and stem cell maintenance in the floral meristem. Genetic analysis shows that termination of the primary shoot meristem in\nl28 mutants requires an active CLV signaling pathway, indicating that AP2 functions in stem cell maintenance by modifying the WUS-CLV3 feedback loop.\n"}