PubMed:9933600
Annnotations
sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 0-81 | Sentence | denotes | A K319N/E325Q double mutant of the lactose permease cotransports H+ with lactose. |
| T2 | 82-142 | Sentence | denotes | Implications for a proposed mechanism of H+/lactose symport. |
| T3 | 143-401 | Sentence | denotes | In this study, we have examined the transport characteristics of the wild-type lactose permease, single mutants in which Lys-319 was changed to asparagine or alanine or Glu-325 was changed to glutamine or alanine, and the corresponding double mutant strains. |
| T4 | 402-590 | Sentence | denotes | The wild-type and Asn-319 mutant showed high levels of lactose uptake, with Km values of 0.42 and 1.30 mM and Vmax values of 102.6 and 48.3 nmol of lactose/min/mg of protein, respectively. |
| T5 | 591-707 | Sentence | denotes | The Asn-319/Gln-325 strain had a normal Km of 0.36 mM and a moderate Vmax of 18.5 nmol of lactose/min/mg of protein. |
| T6 | 708-841 | Sentence | denotes | By comparison, the single E325Q strain had a normal Km of 0.27 mM but a very defective Vmax of 1.3 nmol of lactose/min/mg of protein. |
| T7 | 842-985 | Sentence | denotes | A similar trend was observed among the alanine substitutions at these positions, although the Vmax values were lower for the Ala-319 mutations. |
| T8 | 986-1214 | Sentence | denotes | When comparing the Vmax values between the single position 325 mutants with those of the double mutants, these results indicate that neutral 319 mutations substantially alleviate a defect in Vmax caused by neutral 325 mutations. |
| T9 | 1215-1339 | Sentence | denotes | With regard to H+/lactose coupling, the wild-type permease is normally coupled and can transport lactose against a gradient. |
| T10 | 1340-1502 | Sentence | denotes | The position 325 single mutants showed no evidence of H+ transport with lactose or thiodigalactoside (TDG) and were unable to facilitate uphill lactose transport. |
| T11 | 1503-1625 | Sentence | denotes | The single Asn-319 mutant and double Asn-319/Gln-325 mutant were able to transport H+ upon the addition of lactose or TDG. |
| T12 | 1626-1747 | Sentence | denotes | In addition, both of these strains catalyzed a sugar-dependent H+ leak that inhibited cell growth in the presence of TDG. |
| T13 | 1748-1866 | Sentence | denotes | These two strains were also defective in uphill transport, which may be related to their sugar-dependent leak pathway. |
| T14 | 1867-2094 | Sentence | denotes | Based on these and other results in the literature, a model is presented that describes how the interactions among several ionizable residues within the lactose permease act in a concerted manner to control H+/lactose coupling. |
| T15 | 2095-2241 | Sentence | denotes | In this model, Lys-319 and Glu-325 play a central role in governing the ability of the lactose permease to couple the transport of H+ and lactose. |
GlyCosmos15-Glycan
| Id | Subject | Object | Predicate | Lexical cue | image |
|---|---|---|---|---|---|
| T1 | 35-42 | Glycan | denotes | lactose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE |
| T2 | 73-80 | Glycan | denotes | lactose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE |
| T3 | 126-133 | Glycan | denotes | lactose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE |
| T4 | 222-229 | Glycan | denotes | lactose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE |
| T5 | 457-464 | Glycan | denotes | lactose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE |
| T6 | 550-557 | Glycan | denotes | lactose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE |
| T7 | 681-688 | Glycan | denotes | lactose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE |
| T8 | 815-822 | Glycan | denotes | lactose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE |
| T9 | 1233-1240 | Glycan | denotes | lactose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE |
| T10 | 1312-1319 | Glycan | denotes | lactose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE |
| T11 | 1412-1419 | Glycan | denotes | lactose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE |
| T12 | 1484-1491 | Glycan | denotes | lactose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE |
| T13 | 1610-1617 | Glycan | denotes | lactose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE |
| T14 | 2020-2027 | Glycan | denotes | lactose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE |
| T15 | 2077-2084 | Glycan | denotes | lactose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE |
| T16 | 2182-2189 | Glycan | denotes | lactose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE |
| T17 | 2233-2240 | Glycan | denotes | lactose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE |
GlyCosmos15-Sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 0-81 | Sentence | denotes | A K319N/E325Q double mutant of the lactose permease cotransports H+ with lactose. |
| T2 | 82-142 | Sentence | denotes | Implications for a proposed mechanism of H+/lactose symport. |
| T3 | 143-401 | Sentence | denotes | In this study, we have examined the transport characteristics of the wild-type lactose permease, single mutants in which Lys-319 was changed to asparagine or alanine or Glu-325 was changed to glutamine or alanine, and the corresponding double mutant strains. |
| T4 | 402-590 | Sentence | denotes | The wild-type and Asn-319 mutant showed high levels of lactose uptake, with Km values of 0.42 and 1.30 mM and Vmax values of 102.6 and 48.3 nmol of lactose/min/mg of protein, respectively. |
| T5 | 591-707 | Sentence | denotes | The Asn-319/Gln-325 strain had a normal Km of 0.36 mM and a moderate Vmax of 18.5 nmol of lactose/min/mg of protein. |
| T6 | 708-841 | Sentence | denotes | By comparison, the single E325Q strain had a normal Km of 0.27 mM but a very defective Vmax of 1.3 nmol of lactose/min/mg of protein. |
| T7 | 842-985 | Sentence | denotes | A similar trend was observed among the alanine substitutions at these positions, although the Vmax values were lower for the Ala-319 mutations. |
| T8 | 986-1214 | Sentence | denotes | When comparing the Vmax values between the single position 325 mutants with those of the double mutants, these results indicate that neutral 319 mutations substantially alleviate a defect in Vmax caused by neutral 325 mutations. |
| T9 | 1215-1339 | Sentence | denotes | With regard to H+/lactose coupling, the wild-type permease is normally coupled and can transport lactose against a gradient. |
| T10 | 1340-1502 | Sentence | denotes | The position 325 single mutants showed no evidence of H+ transport with lactose or thiodigalactoside (TDG) and were unable to facilitate uphill lactose transport. |
| T11 | 1503-1625 | Sentence | denotes | The single Asn-319 mutant and double Asn-319/Gln-325 mutant were able to transport H+ upon the addition of lactose or TDG. |
| T12 | 1626-1747 | Sentence | denotes | In addition, both of these strains catalyzed a sugar-dependent H+ leak that inhibited cell growth in the presence of TDG. |
| T13 | 1748-1866 | Sentence | denotes | These two strains were also defective in uphill transport, which may be related to their sugar-dependent leak pathway. |
| T14 | 1867-2094 | Sentence | denotes | Based on these and other results in the literature, a model is presented that describes how the interactions among several ionizable residues within the lactose permease act in a concerted manner to control H+/lactose coupling. |
| T15 | 2095-2241 | Sentence | denotes | In this model, Lys-319 and Glu-325 play a central role in governing the ability of the lactose permease to couple the transport of H+ and lactose. |
Glycan-GlyCosmos
| Id | Subject | Object | Predicate | Lexical cue | image |
|---|---|---|---|---|---|
| T1 | 35-42 | Glycan | denotes | lactose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE |
| T2 | 73-80 | Glycan | denotes | lactose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE |
| T3 | 126-133 | Glycan | denotes | lactose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE |
| T4 | 222-229 | Glycan | denotes | lactose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE |
| T5 | 457-464 | Glycan | denotes | lactose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE |
| T6 | 550-557 | Glycan | denotes | lactose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE |
| T7 | 681-688 | Glycan | denotes | lactose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE |
| T8 | 815-822 | Glycan | denotes | lactose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE |
| T9 | 1233-1240 | Glycan | denotes | lactose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE |
| T10 | 1312-1319 | Glycan | denotes | lactose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE |
| T11 | 1412-1419 | Glycan | denotes | lactose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE |
| T12 | 1484-1491 | Glycan | denotes | lactose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE |
| T13 | 1610-1617 | Glycan | denotes | lactose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE |
| T14 | 2020-2027 | Glycan | denotes | lactose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE |
| T15 | 2077-2084 | Glycan | denotes | lactose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE |
| T16 | 2182-2189 | Glycan | denotes | lactose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE |
| T17 | 2233-2240 | Glycan | denotes | lactose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE |