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PubMed:9857077 JSONTXT

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sentences

Id Subject Object Predicate Lexical cue
T1 0-87 Sentence denotes Functional domains of the alpha1 catalytic subunit of the AMP-activated protein kinase.
T2 88-270 Sentence denotes The AMP-activated protein kinase is a heterotrimeric enzyme, important in cellular adaptation to the stress of nutrient starvation, hypoxia, increased ATP utilization, or heat shock.
T3 271-483 Sentence denotes This mammalian enzyme is composed of a catalytic alpha subunit and noncatalytic beta and gamma subunits and is a member of a larger protein kinase family that includes the SNF1 kinase of Saccharomyces cerevisiae.
T4 484-700 Sentence denotes In the present study, we have identified by truncation and site-directed mutagenesis several functional domains of the alpha1 catalytic subunit, which modulate its activity, subunit association, and protein turnover.
T5 701-887 Sentence denotes C-terminal truncation of the 548-amino acid (aa) wild-type alpha1 protein to aa 312 or 392 abolishes the binding of the beta/gamma subunits and dramatically increases protein expression.
T6 888-1044 Sentence denotes The full-length wild-type alpha1 subunit is only minimally active in the absence of co-expressed beta/gamma, and alpha1(1-392) likewise has little activity.
T7 1045-1373 Sentence denotes Further truncation to aa 312, however, is associated with a large increase in enzyme specific activity, thus revealing an autoinhibitory sequence between aa 313 and 392. alpha-1(1-312) still requires the phosphorylation of the activation loop Thr-172 for enzyme activity, yet is now independent of the allosteric activator, AMP.
T8 1374-1551 Sentence denotes The increased levels of protein expression on transient transfection of either truncated alpha subunit cDNA are because of a decrease in enzyme turnover by pulse-chase analysis.
T9 1552-1715 Sentence denotes Taken together, these data indicate that the alpha1 subunit of AMP-activated protein kinase contains several features that determine enzyme activity and stability.
T10 1716-1910 Sentence denotes A constitutively active form of the kinase that does not require participation by the noncatalytic subunits provides a unique reagent for exploring the functions of AMP-activated protein kinase.

HP-phenotype

Id Subject Object Predicate Lexical cue hp_id
T1 220-227 Phenotype denotes hypoxia HP:0012418
T2 264-269 Phenotype denotes shock HP:0031273

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 458-482 OrganismTaxon denotes Saccharomyces cerevisiae 4932