| Id |
Subject |
Object |
Predicate |
Lexical cue |
| TextSentencer_T1 |
0-149 |
Sentence |
denotes |
Full-length human immunodeficiency virus type 1 genomes from subtype C-infected seroconverters in India, with evidence of intersubtype recombination. |
| TextSentencer_T2 |
150-352 |
Sentence |
denotes |
The development of an effective human immunodeficiency virus type 1 (HIV-1) vaccine is likely to depend on knowledge of circulating variants of genes other than the commonly sequenced gag and env genes. |
| TextSentencer_T3 |
353-500 |
Sentence |
denotes |
In addition, full-genome data are particularly limited for HIV-1 subtype C, currently the most commonly transmitted subtype in India and worldwide. |
| TextSentencer_T4 |
501-626 |
Sentence |
denotes |
Likewise, little is known about sequence variation of HIV-1 in India, the country facing the largest burden of HIV worldwide. |
| TextSentencer_T5 |
627-787 |
Sentence |
denotes |
Therefore, the objective of this study was to clone and characterize the complete genome of HIV-1 from seroconverters infected with subtype C variants in India. |
| TextSentencer_T6 |
788-968 |
Sentence |
denotes |
Cocultured HIV-1 isolates were obtained from six seroincident individuals from Pune, India, and virtually full-length HIV-1 genomes were amplified, cloned, and sequenced from each. |
| TextSentencer_T7 |
969-1234 |
Sentence |
denotes |
Sequence analysis revealed that five of the six genomes were of subtype C, while one was a mosaic of subtypes A and C, with multiple breakpoints in env, nef, and the 3' long terminal repeat as determined by both maximal chi2 analysis and phylogenetic bootstrapping. |
| TextSentencer_T8 |
1235-1323 |
Sentence |
denotes |
Sequences were compared for preservation of known cytotoxic T lymphocyte (CTL) epitopes. |
| TextSentencer_T9 |
1324-1418 |
Sentence |
denotes |
Compared with those of the HIV-1LAI sequence, 38% of well-defined CTL epitopes were identical. |
| TextSentencer_T10 |
1419-1558 |
Sentence |
denotes |
The proportion of nonconservative substitutions for Env, at 61%, was higher (P < 0.001) than those for Gag (24%), Pol (18%), and Nef (32%). |
| TextSentencer_T11 |
1559-1703 |
Sentence |
denotes |
Therefore, characterized CTL epitopes demonstrated substantial differences from subtype B laboratory strains, which were most pronounced in Env. |
| TextSentencer_T12 |
1704-1933 |
Sentence |
denotes |
Because these clones were obtained from Indian seroconverters, they are likely to facilitate vaccine-related efforts in India by providing potential antigens for vaccine candidates as well as for assays of vaccine responsiveness. |
| T1 |
0-149 |
Sentence |
denotes |
Full-length human immunodeficiency virus type 1 genomes from subtype C-infected seroconverters in India, with evidence of intersubtype recombination. |
| T2 |
150-352 |
Sentence |
denotes |
The development of an effective human immunodeficiency virus type 1 (HIV-1) vaccine is likely to depend on knowledge of circulating variants of genes other than the commonly sequenced gag and env genes. |
| T3 |
353-500 |
Sentence |
denotes |
In addition, full-genome data are particularly limited for HIV-1 subtype C, currently the most commonly transmitted subtype in India and worldwide. |
| T4 |
501-626 |
Sentence |
denotes |
Likewise, little is known about sequence variation of HIV-1 in India, the country facing the largest burden of HIV worldwide. |
| T5 |
627-787 |
Sentence |
denotes |
Therefore, the objective of this study was to clone and characterize the complete genome of HIV-1 from seroconverters infected with subtype C variants in India. |
| T6 |
788-968 |
Sentence |
denotes |
Cocultured HIV-1 isolates were obtained from six seroincident individuals from Pune, India, and virtually full-length HIV-1 genomes were amplified, cloned, and sequenced from each. |
| T7 |
969-1234 |
Sentence |
denotes |
Sequence analysis revealed that five of the six genomes were of subtype C, while one was a mosaic of subtypes A and C, with multiple breakpoints in env, nef, and the 3' long terminal repeat as determined by both maximal chi2 analysis and phylogenetic bootstrapping. |
| T8 |
1235-1323 |
Sentence |
denotes |
Sequences were compared for preservation of known cytotoxic T lymphocyte (CTL) epitopes. |
| T9 |
1324-1418 |
Sentence |
denotes |
Compared with those of the HIV-1LAI sequence, 38% of well-defined CTL epitopes were identical. |
| T10 |
1419-1558 |
Sentence |
denotes |
The proportion of nonconservative substitutions for Env, at 61%, was higher (P < 0.001) than those for Gag (24%), Pol (18%), and Nef (32%). |
| T11 |
1559-1703 |
Sentence |
denotes |
Therefore, characterized CTL epitopes demonstrated substantial differences from subtype B laboratory strains, which were most pronounced in Env. |
| T12 |
1704-1933 |
Sentence |
denotes |
Because these clones were obtained from Indian seroconverters, they are likely to facilitate vaccine-related efforts in India by providing potential antigens for vaccine candidates as well as for assays of vaccine responsiveness. |