PubMed:9827568 JSONTXT

{"project":"bionlp-st-gro-2013-development","denotations":[{"id":"T1","span":{"begin":26,"end":31},"obj":"Eukaryote"},{"id":"T2","span":{"begin":32,"end":47},"obj":"Gene"},{"id":"T3","span":{"begin":122,"end":134},"obj":"HMGTF"},{"id":"T4","span":{"begin":155,"end":158},"obj":"HMG"},{"id":"T5","span":{"begin":167,"end":185},"obj":"DNABindingDomainOfProtein"},{"id":"T7","span":{"begin":246,"end":253},"obj":"Eukaryote"},{"id":"T9","span":{"begin":276,"end":285},"obj":"Gene"},{"id":"T10","span":{"begin":310,"end":315},"obj":"Eukaryote"},{"id":"T12","span":{"begin":404,"end":407},"obj":"Gene"},{"id":"T13","span":{"begin":408,"end":415},"obj":"HMG"},{"id":"T14","span":{"begin":480,"end":483},"obj":"Gene"},{"id":"T15","span":{"begin":484,"end":493},"obj":"HMG"},{"id":"T16","span":{"begin":499,"end":504},"obj":"Eukaryote"},{"id":"T17","span":{"begin":513,"end":516},"obj":"DNA"},{"id":"T18","span":{"begin":557,"end":572},"obj":"Gene"},{"id":"T19","span":{"begin":416,"end":425},"obj":"Sequence"},{"id":"E1","span":{"begin":231,"end":242},"obj":"DevelopmentalProcess"},{"id":"E2","span":{"begin":255,"end":264},"obj":"Mutation"},{"id":"E3","span":{"begin":316,"end":324},"obj":"Disease"}],"relations":[{"id":"R1","pred":"fromSpecies","subj":"T2","obj":"T1"},{"id":"R2","pred":"hasPart","subj":"T3","obj":"T5"},{"id":"R3","pred":"fromSpecies","subj":"E1","obj":"T7"},{"id":"R4","pred":"fromSpecies","subj":"E3","obj":"T10"},{"id":"R6","pred":"hasPart","subj":"T14","obj":"T15"},{"id":"R7","pred":"fromSpecies","subj":"T17","obj":"T16"},{"id":"R5","pred":"hasPart","subj":"T12","obj":"T19"},{"id":"R7","pred":"hasPatient","subj":"T9","obj":"E2"}],"text":"Further complexity of the human SOX gene family revealed by the combined use of highly degenerate primers and nested PCR.\nSOX proteins contain a conserved HMG-related DNA-binding domain. They fulfill essential functions during the development of animals. Mutations in several SOX genes have been implicated in human diseases. We present here a new set of PCR primers designed to amplify a broad range of SOX HMG-box sequences. These primers facilitated the cloning of several new SOX HMG boxes from human genomic DNA, revealing unexpected complexity of the SOX gene family."}