PubMed:9813075 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/9813075","sourcedb":"PubMed","sourceid":"9813075","source_url":"https://www.ncbi.nlm.nih.gov/pubmed/9813075","text":"Pathway of complex formation between DNA and three subunits of CBF/NF-Y. Photocross-linking analysis of DNA-protein interaction and characterization of equilibrium steps of subunit interaction and dna binding.\nIn this study, we used a photocross-linking method to identify specific contact of CCAAT-binding factor (CBF) subunits in a CBF-DNA complex. The analysis showed that all three subunits in the CBF-DNA complex were cross-linked to DNA and that CBF-B and CBF-C were cross-linked more strongly than CBF-A. None of the CBF-A and CBF-C subunits, which together formed a CBF-A/CBF-C heterodimer, were cross-linked without CBF-B; in contrast, CBF-B was cross-linked in the absence of CBF-A/CBF-C. No subunit of heterotrimeric CBF containing DNA-binding domain mutant of either CBF-B or CBF-C was cross-linked to DNA, and interestingly, cross-linking of CBF-B that occurred without CBF-A/CBF-C was inhibited in presence of mutant CBF-C/CBF-A heterodimer. Altogether, these results indicated that the specific DNA contact surface of each CBF subunit is generated as a result of interaction between CBF-B and CBF-A/CBF-C heterodimer and that the three CBF subunits interact interdependently with DNA to form a CBF-DNA complex. Equilibrium interactions among the three CBF subunits and between CBF subunits and DNA were studied by electrophoretic mobility shift assay. This showed that at equilibrium DNA-binding conditions, the CBF-A/CBF-C heterodimer is very stable, but association between CBF-B and CBF-A/CBF-C is very weak. The nature of the association of CBF-B with CBF-A/CBF-C was also revealed by studying the inhibition of CBF-DNA complex formation by the mutant CBF-B. This study indicated that the association between CBF-B and CBF-A/CBF-C is stabilized upon interaction with DNA, a process likely to favor formation of a high-affinity CBF-DNA complex.","tracks":[{"project":"sentences","denotations":[{"id":"T1","span":{"begin":0,"end":72},"obj":"Sentence"},{"id":"T2","span":{"begin":73,"end":209},"obj":"Sentence"},{"id":"T3","span":{"begin":210,"end":350},"obj":"Sentence"},{"id":"T4","span":{"begin":351,"end":511},"obj":"Sentence"},{"id":"T5","span":{"begin":512,"end":698},"obj":"Sentence"},{"id":"T6","span":{"begin":699,"end":955},"obj":"Sentence"},{"id":"T7","span":{"begin":956,"end":1225},"obj":"Sentence"},{"id":"T8","span":{"begin":1226,"end":1366},"obj":"Sentence"},{"id":"T9","span":{"begin":1367,"end":1526},"obj":"Sentence"},{"id":"T10","span":{"begin":1527,"end":1677},"obj":"Sentence"},{"id":"T11","span":{"begin":1678,"end":1862},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":72},"obj":"Sentence"},{"id":"T2","span":{"begin":73,"end":209},"obj":"Sentence"},{"id":"T3","span":{"begin":210,"end":350},"obj":"Sentence"},{"id":"T4","span":{"begin":351,"end":511},"obj":"Sentence"},{"id":"T5","span":{"begin":512,"end":698},"obj":"Sentence"},{"id":"T6","span":{"begin":699,"end":955},"obj":"Sentence"},{"id":"T7","span":{"begin":956,"end":1225},"obj":"Sentence"},{"id":"T8","span":{"begin":1226,"end":1366},"obj":"Sentence"},{"id":"T9","span":{"begin":1367,"end":1526},"obj":"Sentence"},{"id":"T10","span":{"begin":1527,"end":1677},"obj":"Sentence"},{"id":"T11","span":{"begin":1678,"end":1862},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"},{"subj":"T5","pred":"source","obj":"sentences"},{"subj":"T6","pred":"source","obj":"sentences"},{"subj":"T7","pred":"source","obj":"sentences"},{"subj":"T8","pred":"source","obj":"sentences"},{"subj":"T9","pred":"source","obj":"sentences"},{"subj":"T10","pred":"source","obj":"sentences"},{"subj":"T11","pred":"source","obj":"sentences"},{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"},{"subj":"T5","pred":"source","obj":"sentences"},{"subj":"T6","pred":"source","obj":"sentences"},{"subj":"T7","pred":"source","obj":"sentences"},{"subj":"T8","pred":"source","obj":"sentences"},{"subj":"T9","pred":"source","obj":"sentences"},{"subj":"T10","pred":"source","obj":"sentences"},{"subj":"T11","pred":"source","obj":"sentences"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"sentences","color":"#b7ec93","default":true}]}]}}