PubMed:9751710 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/9751710","sourcedb":"PubMed","sourceid":"9751710","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/9751710","text":"Transcriptional repression by AML1 and LEF-1 is mediated by the TLE/Groucho corepressors.\nThe mammalian AML/CBFalpha runt domain (RD) transcription factors regulate hematopoiesis and osteoblast differentiation. Like their Drosophila counterparts, most mammalian RD proteins terminate in a common pentapeptide, VWRPY, which serves to recruit the corepressor Groucho (Gro). Using a yeast two-hybrid assay, in vitro association and pull-down experiments, we demonstrate that Gro and its mammalian homolog TLE1 specifically interact with AML1 and AML2. In addition to the VWRPY motif, other C-terminal sequences are required for these interactions with Gro/TLE1. TLE1 inhibits AML1-dependent transactivation of the T cell receptor (TCR) enhancers alpha and beta, which contain functional AML binding sites, in transfected Jurkat T cells. LEF-1 is an additional transcription factor that mediates transactivation of TCR enhancers. LEF-1 and its Drosophila homolog Pangolin (Pan) are involved in the Wnt/Wg signaling pathway through interactions with the coactivator beta-catenin and its highly conserved fly homolog Armadillo (Arm). We show that TLE/Gro interacts with LEF-1 and Pan, and inhibits LEF-1:beta-catenin-dependent transcription. These data indicate that, in addition to their activity as transcriptional activators, AML1 and LEF-1 can act, through recruitment of the corepressor TLE1, as transcriptional repressors in TCR regulation and Wnt/Wg signaling.","tracks":[{"project":"relna","denotations":[{"id":"T1","span":{"begin":30,"end":34},"obj":"DNA"},{"id":"T2","span":{"begin":39,"end":44},"obj":"DNA"},{"id":"T3","span":{"begin":502,"end":506},"obj":"Protein"},{"id":"T4","span":{"begin":534,"end":538},"obj":"DNA"},{"id":"T5","span":{"begin":543,"end":547},"obj":"DNA"},{"id":"T6","span":{"begin":653,"end":657},"obj":"Protein"},{"id":"T7","span":{"begin":659,"end":663},"obj":"Protein"},{"id":"T8","span":{"begin":673,"end":677},"obj":"DNA"},{"id":"T9","span":{"begin":834,"end":839},"obj":"DNA"},{"id":"T10","span":{"begin":926,"end":931},"obj":"DNA"},{"id":"T11","span":{"begin":959,"end":967},"obj":"DNA"},{"id":"T12","span":{"begin":969,"end":972},"obj":"DNA"},{"id":"T13","span":{"begin":1061,"end":1073},"obj":"Protein"},{"id":"T14","span":{"begin":1111,"end":1120},"obj":"Protein"},{"id":"T15","span":{"begin":1122,"end":1125},"obj":"Protein"},{"id":"T16","span":{"begin":1164,"end":1169},"obj":"DNA"},{"id":"T17","span":{"begin":1192,"end":1197},"obj":"DNA"},{"id":"T18","span":{"begin":1198,"end":1210},"obj":"Protein"},{"id":"T19","span":{"begin":1323,"end":1327},"obj":"DNA"},{"id":"T20","span":{"begin":1332,"end":1337},"obj":"DNA"},{"id":"T21","span":{"begin":1386,"end":1390},"obj":"Protein"}],"relations":[{"id":"R0","pred":"linked","subj":"T3","obj":"T4"},{"id":"R1","pred":"linked","subj":"T3","obj":"T5"},{"id":"R2","pred":"linked","subj":"T10","obj":"T13"},{"id":"R3","pred":"linked","subj":"T11","obj":"T14"},{"id":"R4","pred":"linked","subj":"T11","obj":"T15"},{"id":"R5","pred":"linked","subj":"T12","obj":"T14"},{"id":"R6","pred":"linked","subj":"T12","obj":"T15"}],"attributes":[{"subj":"T1","pred":"source","obj":"relna"},{"subj":"T2","pred":"source","obj":"relna"},{"subj":"T3","pred":"source","obj":"relna"},{"subj":"T4","pred":"source","obj":"relna"},{"subj":"T5","pred":"source","obj":"relna"},{"subj":"T6","pred":"source","obj":"relna"},{"subj":"T7","pred":"source","obj":"relna"},{"subj":"T8","pred":"source","obj":"relna"},{"subj":"T9","pred":"source","obj":"relna"},{"subj":"T10","pred":"source","obj":"relna"},{"subj":"T11","pred":"source","obj":"relna"},{"subj":"T12","pred":"source","obj":"relna"},{"subj":"T13","pred":"source","obj":"relna"},{"subj":"T14","pred":"source","obj":"relna"},{"subj":"T15","pred":"source","obj":"relna"},{"subj":"T16","pred":"source","obj":"relna"},{"subj":"T17","pred":"source","obj":"relna"},{"subj":"T18","pred":"source","obj":"relna"},{"subj":"T19","pred":"source","obj":"relna"},{"subj":"T20","pred":"source","obj":"relna"},{"subj":"T21","pred":"source","obj":"relna"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"relna","color":"#93ecb0","default":true}]}]}}