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PubMed:9675218 JSONTXT

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Glycan-Motif

Id Subject Object Predicate Lexical cue
T1 763-769 https://glytoucan.org/Structures/Glycans/G81533KY denotes Neu5Ac
T2 1018-1025 https://glytoucan.org/Structures/Glycans/G00021MO denotes Heparin
T3 1018-1025 https://glytoucan.org/Structures/Glycans/G54161DR denotes Heparin
T4 1146-1153 https://glytoucan.org/Structures/Glycans/G00021MO denotes heparin
T5 1146-1153 https://glytoucan.org/Structures/Glycans/G54161DR denotes heparin
T6 1226-1233 https://glytoucan.org/Structures/Glycans/G00021MO denotes heparin
T7 1226-1233 https://glytoucan.org/Structures/Glycans/G54161DR denotes heparin
T8 1909-1916 https://glytoucan.org/Structures/Glycans/G00021MO denotes heparin
T9 1909-1916 https://glytoucan.org/Structures/Glycans/G54161DR denotes heparin

GlyCosmos6-Glycan-Motif-Image

Id Subject Object Predicate Lexical cue image
T1 763-769 Glycan_Motif denotes Neu5Ac https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81533KY
T2 1018-1025 Glycan_Motif denotes Heparin https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G54161DR|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00021MO
T4 1146-1153 Glycan_Motif denotes heparin https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G54161DR|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00021MO
T6 1226-1233 Glycan_Motif denotes heparin https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G54161DR|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00021MO
T8 1909-1916 Glycan_Motif denotes heparin https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G54161DR|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00021MO

sentences

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-118 Sentence denotes Developmental expression and characterization of the alpha2,8-polysialyltransferase activity in embryonic chick brain.
TextSentencer_T2 119-293 Sentence denotes The alpha2,8-polysialyltransferases (polySTs) from embryonic chick brain catalyze the alpha2,8-specific polysialylation of endogenous neural cell adhesion molecules (N-CAMs).
TextSentencer_T3 294-383 Sentence denotes This posttranslation glycosylation decreases N-CAM-dependent cell adhesion and migration.
TextSentencer_T4 384-485 Sentence denotes The enzymatic properties of the membrane-bound form of the polyST activity was investigated in vitro.
TextSentencer_T5 486-633 Sentence denotes Our results show that the polyST activity was developmentally expressed with maximum specific activity appearing about 12 days after fertilization.
TextSentencer_T6 634-843 Sentence denotes This time shortly precedes maximal expression of the cognate polysialylated N-CAMs. Kinetic studies showed the KMand Vmaxfor CMP-Neu5Ac were 133 microM and 0.13 microM/h, respectively, at pH 6.1, 33 degrees C.
TextSentencer_T7 844-881 Sentence denotes CMP-Neu5Gc was not a donor substrate.
TextSentencer_T8 882-1017 Sentence denotes PolyST activity was increased 5- to 6-fold in the presence of 10 mM MnCl2,the preferred divalent cation, and 1 mM dithiothreitol (DTT).
TextSentencer_T9 1018-1105 Sentence denotes Heparin (3 kDa) was a noncompetitive inhibitor of polysialylation with a Kiof 9 microM.
TextSentencer_T10 1106-1342 Sentence denotes Based on the affinity of the enzyme for heparin, the polyST activity was partially purified ( approximately 30-fold) by heparin-Sepharose affinity chromatography, after differential solubilization with the zwitterionic detergent, CHAPS.
TextSentencer_T11 1343-1722 Sentence denotes DTT and chemical modification studies using the thiol-directed alkylating reagents, N-ethylmaleimide (NEM) and iodoacetamide (IAA), were used to show that at least one cysteinyl residue in the polyST was of critical importance for polysialylation, but of lesser importance for monosialylation, catalyzed by the alpha2,3-, alpha2,6-, and alpha2,8-monosialyltransferases (monoSTs).
TextSentencer_T12 1723-1778 Sentence denotes A sulfhydryl residue is implicated in chain initiation.
TextSentencer_T13 1779-1881 Sentence denotes Two important structural differences between the mono- and polySTs were revealed by sequence analyses.
TextSentencer_T14 1882-1999 Sentence denotes First, the polySTs contain heparin-like, positively charged amino acid clusters upstream of both sialylmotif L and S.
TextSentencer_T15 2000-2079 Sentence denotes Second, the polySTs contain a uniquely extended basic amino acid region (pI 11.
TextSentencer_T16 2080-2141 Sentence denotes 6-12.0) of 31 residues immediately upstream of sialylmotif S.
TextSentencer_T17 2142-2410 Sentence denotes This extended, positively charged region may function in the processive mechanism of polymerization by allowing nascent polySia chains to remain bound to the polyST during the repetitive addition of each new Sia residue to the nonreducing termini of the growing chain.
TextSentencer_T18 2411-2522 Sentence denotes The importance of these studies is that they provide new information on the enzymatic basis of polysialylation.
TextSentencer_T19 2523-2689 Sentence denotes They also reveal that sulfhydryl residues and extended basic amino acid domains are two structural features unique to polysialylation, in contrast to monosialylation.
TextSentencer_T20 2690-2862 Sentence denotes Both may be important distinguishing features between the classes of distributive (monoSTs) and processive polysialyltransferases, which have not been previously described.
T1 0-118 Sentence denotes Developmental expression and characterization of the alpha2,8-polysialyltransferase activity in embryonic chick brain.
T2 119-293 Sentence denotes The alpha2,8-polysialyltransferases (polySTs) from embryonic chick brain catalyze the alpha2,8-specific polysialylation of endogenous neural cell adhesion molecules (N-CAMs).
T3 294-383 Sentence denotes This posttranslation glycosylation decreases N-CAM-dependent cell adhesion and migration.
T4 384-485 Sentence denotes The enzymatic properties of the membrane-bound form of the polyST activity was investigated in vitro.
T5 486-633 Sentence denotes Our results show that the polyST activity was developmentally expressed with maximum specific activity appearing about 12 days after fertilization.
T6 634-881 Sentence denotes This time shortly precedes maximal expression of the cognate polysialylated N-CAMs. Kinetic studies showed the KMand Vmaxfor CMP-Neu5Ac were 133 microM and 0.13 microM/h, respectively, at pH 6.1, 33 degrees C. CMP-Neu5Gc was not a donor substrate.
T7 882-1017 Sentence denotes PolyST activity was increased 5- to 6-fold in the presence of 10 mM MnCl2,the preferred divalent cation, and 1 mM dithiothreitol (DTT).
T8 1018-1105 Sentence denotes Heparin (3 kDa) was a noncompetitive inhibitor of polysialylation with a Kiof 9 microM.
T9 1106-1342 Sentence denotes Based on the affinity of the enzyme for heparin, the polyST activity was partially purified ( approximately 30-fold) by heparin-Sepharose affinity chromatography, after differential solubilization with the zwitterionic detergent, CHAPS.
T10 1343-1722 Sentence denotes DTT and chemical modification studies using the thiol-directed alkylating reagents, N-ethylmaleimide (NEM) and iodoacetamide (IAA), were used to show that at least one cysteinyl residue in the polyST was of critical importance for polysialylation, but of lesser importance for monosialylation, catalyzed by the alpha2,3-, alpha2,6-, and alpha2,8-monosialyltransferases (monoSTs).
T11 1723-1778 Sentence denotes A sulfhydryl residue is implicated in chain initiation.
T12 1779-1881 Sentence denotes Two important structural differences between the mono- and polySTs were revealed by sequence analyses.
T13 1882-2079 Sentence denotes First, the polySTs contain heparin-like, positively charged amino acid clusters upstream of both sialylmotif L and S. Second, the polySTs contain a uniquely extended basic amino acid region (pI 11.
T14 2080-2410 Sentence denotes 6-12.0) of 31 residues immediately upstream of sialylmotif S. This extended, positively charged region may function in the processive mechanism of polymerization by allowing nascent polySia chains to remain bound to the polyST during the repetitive addition of each new Sia residue to the nonreducing termini of the growing chain.
T15 2411-2522 Sentence denotes The importance of these studies is that they provide new information on the enzymatic basis of polysialylation.
T16 2523-2689 Sentence denotes They also reveal that sulfhydryl residues and extended basic amino acid domains are two structural features unique to polysialylation, in contrast to monosialylation.
T17 2690-2862 Sentence denotes Both may be important distinguishing features between the classes of distributive (monoSTs) and processive polysialyltransferases, which have not been previously described.
T1 0-118 Sentence denotes Developmental expression and characterization of the alpha2,8-polysialyltransferase activity in embryonic chick brain.
T2 119-293 Sentence denotes The alpha2,8-polysialyltransferases (polySTs) from embryonic chick brain catalyze the alpha2,8-specific polysialylation of endogenous neural cell adhesion molecules (N-CAMs).
T3 294-383 Sentence denotes This posttranslation glycosylation decreases N-CAM-dependent cell adhesion and migration.
T4 384-485 Sentence denotes The enzymatic properties of the membrane-bound form of the polyST activity was investigated in vitro.
T5 486-633 Sentence denotes Our results show that the polyST activity was developmentally expressed with maximum specific activity appearing about 12 days after fertilization.
T6 634-843 Sentence denotes This time shortly precedes maximal expression of the cognate polysialylated N-CAMs. Kinetic studies showed the KMand Vmaxfor CMP-Neu5Ac were 133 microM and 0.13 microM/h, respectively, at pH 6.1, 33 degrees C.
T7 844-881 Sentence denotes CMP-Neu5Gc was not a donor substrate.
T8 882-1017 Sentence denotes PolyST activity was increased 5- to 6-fold in the presence of 10 mM MnCl2,the preferred divalent cation, and 1 mM dithiothreitol (DTT).
T9 1018-1105 Sentence denotes Heparin (3 kDa) was a noncompetitive inhibitor of polysialylation with a Kiof 9 microM.
T10 1106-1342 Sentence denotes Based on the affinity of the enzyme for heparin, the polyST activity was partially purified ( approximately 30-fold) by heparin-Sepharose affinity chromatography, after differential solubilization with the zwitterionic detergent, CHAPS.
T11 1343-1722 Sentence denotes DTT and chemical modification studies using the thiol-directed alkylating reagents, N-ethylmaleimide (NEM) and iodoacetamide (IAA), were used to show that at least one cysteinyl residue in the polyST was of critical importance for polysialylation, but of lesser importance for monosialylation, catalyzed by the alpha2,3-, alpha2,6-, and alpha2,8-monosialyltransferases (monoSTs).
T12 1723-1778 Sentence denotes A sulfhydryl residue is implicated in chain initiation.
T13 1779-1881 Sentence denotes Two important structural differences between the mono- and polySTs were revealed by sequence analyses.
T14 1882-1999 Sentence denotes First, the polySTs contain heparin-like, positively charged amino acid clusters upstream of both sialylmotif L and S.
T15 2000-2079 Sentence denotes Second, the polySTs contain a uniquely extended basic amino acid region (pI 11.
T16 2080-2141 Sentence denotes 6-12.0) of 31 residues immediately upstream of sialylmotif S.
T17 2142-2410 Sentence denotes This extended, positively charged region may function in the processive mechanism of polymerization by allowing nascent polySia chains to remain bound to the polyST during the repetitive addition of each new Sia residue to the nonreducing termini of the growing chain.
T18 2411-2522 Sentence denotes The importance of these studies is that they provide new information on the enzymatic basis of polysialylation.
T19 2523-2689 Sentence denotes They also reveal that sulfhydryl residues and extended basic amino acid domains are two structural features unique to polysialylation, in contrast to monosialylation.
T20 2690-2862 Sentence denotes Both may be important distinguishing features between the classes of distributive (monoSTs) and processive polysialyltransferases, which have not been previously described.

GlyCosmos6-Glycan-Motif-Structure

Id Subject Object Predicate Lexical cue
T1 763-769 https://glytoucan.org/Structures/Glycans/G81533KY denotes Neu5Ac
T2 1018-1025 https://glytoucan.org/Structures/Glycans/G00021MO denotes Heparin
T3 1018-1025 https://glytoucan.org/Structures/Glycans/G54161DR denotes Heparin
T4 1146-1153 https://glytoucan.org/Structures/Glycans/G00021MO denotes heparin
T5 1146-1153 https://glytoucan.org/Structures/Glycans/G54161DR denotes heparin
T6 1226-1233 https://glytoucan.org/Structures/Glycans/G00021MO denotes heparin
T7 1226-1233 https://glytoucan.org/Structures/Glycans/G54161DR denotes heparin
T8 1909-1916 https://glytoucan.org/Structures/Glycans/G00021MO denotes heparin
T9 1909-1916 https://glytoucan.org/Structures/Glycans/G54161DR denotes heparin

Glycosmos6-MAT

Id Subject Object Predicate Lexical cue
T1 112-117 http://purl.obolibrary.org/obo/MAT_0000098 denotes brain
T2 186-191 http://purl.obolibrary.org/obo/MAT_0000098 denotes brain

GlycoBiology-FMA

Id Subject Object Predicate Lexical cue
_T1 112-117 FMAID:146514 denotes brain
_T2 112-117 FMAID:50801 denotes brain
_T3 186-191 FMAID:50801 denotes brain
_T4 186-191 FMAID:146514 denotes brain
_T5 253-283 FMAID:67856 denotes neural cell adhesion molecules
_T6 253-283 FMAID:165776 denotes neural cell adhesion molecules
_T7 260-283 FMAID:165233 denotes cell adhesion molecules
_T8 260-283 FMAID:67214 denotes cell adhesion molecules
_T9 260-283 FMAID:62914 denotes cell adhesion molecules
_T10 260-283 FMAID:167238 denotes cell adhesion molecules
_T11 260-283 FMAID:167237 denotes cell adhesion molecules
_T12 260-283 FMAID:62915 denotes cell adhesion molecules
_T13 285-291 FMAID:165778 denotes N-CAMs
_T14 287-291 FMAID:165234 denotes CAMs
_T15 339-344 FMAID:165778 denotes N-CAM
_T16 341-344 FMAID:165234 denotes CAM
_T17 710-716 FMAID:165778 denotes N-CAMs
_T18 712-716 FMAID:165234 denotes CAMs
_T19 779-785 FMAID:165145 denotes microM
_T20 795-801 FMAID:165145 denotes microM
_T21 1018-1025 FMAID:82839 denotes Heparin
_T22 1018-1025 FMAID:167420 denotes Heparin
_T23 1098-1104 FMAID:165145 denotes microM
_T24 1146-1153 FMAID:167420 denotes heparin
_T25 1146-1153 FMAID:82839 denotes heparin
_T26 1226-1233 FMAID:82839 denotes heparin
_T27 1226-1233 FMAID:167420 denotes heparin
_T28 1909-1916 FMAID:82839 denotes heparin
_T29 1909-1916 FMAID:167420 denotes heparin
_T30 1942-1952 FMAID:82739 denotes amino acid
_T31 1942-1952 FMAID:196728 denotes amino acid
_T32 2054-2064 FMAID:196728 denotes amino acid
_T33 2054-2064 FMAID:82739 denotes amino acid
_T34 2073-2075 FMAID:214745 denotes pI
_T35 2584-2594 FMAID:82739 denotes amino acid
_T36 2584-2594 FMAID:196728 denotes amino acid

uniprot-human

Id Subject Object Predicate Lexical cue
T1 260-283 http://www.uniprot.org/uniprot/P16422 denotes cell adhesion molecules
T2 260-283 http://www.uniprot.org/uniprot/P43121 denotes cell adhesion molecules
T3 260-283 http://www.uniprot.org/uniprot/Q14CZ8 denotes cell adhesion molecules
T4 260-283 http://www.uniprot.org/uniprot/Q8N126 denotes cell adhesion molecules
T5 260-283 http://www.uniprot.org/uniprot/Q8NFZ8 denotes cell adhesion molecules
T6 260-283 http://www.uniprot.org/uniprot/P50895 denotes cell adhesion molecules
T7 260-283 http://www.uniprot.org/uniprot/Q8N3J6 denotes cell adhesion molecules
T8 260-283 http://www.uniprot.org/uniprot/Q8N2F4 denotes cell adhesion molecules
T9 260-283 http://www.uniprot.org/uniprot/Q92823 denotes cell adhesion molecules
T10 341-344 http://www.uniprot.org/uniprot/Q96HK3 denotes CAM
T11 759-762 http://www.uniprot.org/uniprot/P21941 denotes CMP
T12 844-847 http://www.uniprot.org/uniprot/P21941 denotes CMP

GlycoBiology-NCBITAXON

Id Subject Object Predicate Lexical cue
T1 608-612 http://purl.bioontology.org/ontology/NCBITAXON/82391 denotes days
T2 970-978 http://purl.bioontology.org/ontology/NCBITAXON/433098 denotes divalent
T3 2214-2223 http://purl.bioontology.org/ontology/NCBITAXON/127244 denotes mechanism
T4 2350-2353 http://purl.bioontology.org/ontology/NCBITAXON/208766 denotes Sia
T5 2350-2353 http://purl.bioontology.org/ontology/NCBITAXON/172412 denotes Sia

GO-BP

Id Subject Object Predicate Lexical cue
T1 260-273 http://purl.obolibrary.org/obo/GO_0007155 denotes cell adhesion
T2 355-368 http://purl.obolibrary.org/obo/GO_0007155 denotes cell adhesion
T3 260-283 http://purl.obolibrary.org/obo/GO_0060352 denotes cell adhesion molecules
T4 260-283 http://purl.obolibrary.org/obo/GO_0007155 denotes cell adhesion molecules
T5 315-328 http://purl.obolibrary.org/obo/GO_0070085 denotes glycosylation
T6 619-632 http://purl.obolibrary.org/obo/GO_0009566 denotes fertilization

GO-CC

Id Subject Object Predicate Lexical cue
T1 260-264 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T2 355-359 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T3 416-424 http://purl.obolibrary.org/obo/GO_0016020 denotes membrane

UBERON-AE

Id Subject Object Predicate Lexical cue
T1 112-117 http://purl.obolibrary.org/obo/UBERON_0000955 denotes brain
T2 186-191 http://purl.obolibrary.org/obo/UBERON_0000955 denotes brain

GlycoBiology-MAT

Id Subject Object Predicate Lexical cue
T1 112-117 http://purl.obolibrary.org/obo/MAT_0000098 denotes brain
T2 186-191 http://purl.obolibrary.org/obo/MAT_0000098 denotes brain

GlycoBiology-Motifs

Id Subject Object Predicate Lexical cue
T1 1146-1153 http://rdf.glycoinfo.org/glycan/G54161DR denotes heparin
T2 1226-1233 http://rdf.glycoinfo.org/glycan/G54161DR denotes heparin
T3 1909-1916 http://rdf.glycoinfo.org/glycan/G54161DR denotes heparin

GlyTouCan-IUPAC

Id Subject Object Predicate Lexical cue
GlycanIUPAC_T1 763-769 "http://rdf.glycoinfo.org/glycan/G76685HR" denotes Neu5Ac
GlycanIUPAC_T2 763-769 "http://rdf.glycoinfo.org/glycan/G65881BF" denotes Neu5Ac
GlycanIUPAC_T3 763-769 "http://rdf.glycoinfo.org/glycan/G82702MH" denotes Neu5Ac
GlycanIUPAC_T4 763-769 "http://rdf.glycoinfo.org/glycan/G51494MY" denotes Neu5Ac
GlycanIUPAC_T5 763-769 "http://rdf.glycoinfo.org/glycan/G37109XL" denotes Neu5Ac
GlycanIUPAC_T6 763-769 "http://rdf.glycoinfo.org/glycan/G47427MX" denotes Neu5Ac
GlycanIUPAC_T7 763-769 "http://rdf.glycoinfo.org/glycan/G89927NS" denotes Neu5Ac
GlycanIUPAC_T8 848-854 "http://rdf.glycoinfo.org/glycan/G37754MA" denotes Neu5Gc

performance-test

Id Subject Object Predicate Lexical cue
PD-UBERON-AE-B_T1 112-117 http://purl.obolibrary.org/obo/UBERON_0000955 denotes brain
PD-UBERON-AE-B_T2 186-191 http://purl.obolibrary.org/obo/UBERON_0000955 denotes brain

Anatomy-MAT

Id Subject Object Predicate Lexical cue mat_id
T1 112-117 Body_part denotes brain http://purl.obolibrary.org/obo/MAT_0000098
T2 186-191 Body_part denotes brain http://purl.obolibrary.org/obo/MAT_0000098

mondo_disease

Id Subject Object Predicate Lexical cue mondo_id
T1 287-291 Disease denotes CAMs http://purl.obolibrary.org/obo/MONDO_0015405
T2 712-716 Disease denotes CAMs http://purl.obolibrary.org/obo/MONDO_0015405

Glycan-GlyCosmos

Id Subject Object Predicate Lexical cue image
T1 763-769 Glycan denotes Neu5Ac https://api.glycosmos.org/wurcs2image/latest/png/binary/G76685HR

GlyCosmos15-MONDO

Id Subject Object Predicate Lexical cue mondo_id
T1 287-291 Disease denotes CAMs http://purl.obolibrary.org/obo/MONDO_0015405
T2 712-716 Disease denotes CAMs http://purl.obolibrary.org/obo/MONDO_0015405

GlyCosmos15-CL

Id Subject Object Predicate Lexical cue cl_id
T1 253-264 Cell denotes neural cell http://purl.obolibrary.org/obo/CL:0002319

GlyCosmos15-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 112-117 Body_part denotes brain http://purl.obolibrary.org/obo/UBERON_0000955|http://purl.obolibrary.org/obo/UBERON_6110636
T3 186-191 Body_part denotes brain http://purl.obolibrary.org/obo/UBERON_0000955|http://purl.obolibrary.org/obo/UBERON_6110636
T5 253-264 Body_part denotes neural cell http://purl.obolibrary.org/obo/CL_0002319
T6 416-424 Body_part denotes membrane http://purl.obolibrary.org/obo/GO_0016020|http://purl.obolibrary.org/obo/UBERON_0000094|http://purl.obolibrary.org/obo/UBERON_0000158

GlyCosmos15-MAT

Id Subject Object Predicate Lexical cue mat_id
T1 112-117 Body_part denotes brain http://purl.obolibrary.org/obo/MAT_0000098
T2 186-191 Body_part denotes brain http://purl.obolibrary.org/obo/MAT_0000098

sentences

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-118 Sentence denotes Developmental expression and characterization of the alpha2,8-polysialyltransferase activity in embryonic chick brain.
TextSentencer_T2 119-293 Sentence denotes The alpha2,8-polysialyltransferases (polySTs) from embryonic chick brain catalyze the alpha2,8-specific polysialylation of endogenous neural cell adhesion molecules (N-CAMs).
TextSentencer_T3 294-383 Sentence denotes This posttranslation glycosylation decreases N-CAM-dependent cell adhesion and migration.
TextSentencer_T4 384-485 Sentence denotes The enzymatic properties of the membrane-bound form of the polyST activity was investigated in vitro.
TextSentencer_T5 486-633 Sentence denotes Our results show that the polyST activity was developmentally expressed with maximum specific activity appearing about 12 days after fertilization.
TextSentencer_T6 634-843 Sentence denotes This time shortly precedes maximal expression of the cognate polysialylated N-CAMs. Kinetic studies showed the KMand Vmaxfor CMP-Neu5Ac were 133 microM and 0.13 microM/h, respectively, at pH 6.1, 33 degrees C.
TextSentencer_T7 844-881 Sentence denotes CMP-Neu5Gc was not a donor substrate.
TextSentencer_T8 882-1017 Sentence denotes PolyST activity was increased 5- to 6-fold in the presence of 10 mM MnCl2,the preferred divalent cation, and 1 mM dithiothreitol (DTT).
TextSentencer_T9 1018-1105 Sentence denotes Heparin (3 kDa) was a noncompetitive inhibitor of polysialylation with a Kiof 9 microM.
TextSentencer_T10 1106-1342 Sentence denotes Based on the affinity of the enzyme for heparin, the polyST activity was partially purified ( approximately 30-fold) by heparin-Sepharose affinity chromatography, after differential solubilization with the zwitterionic detergent, CHAPS.
TextSentencer_T11 1343-1722 Sentence denotes DTT and chemical modification studies using the thiol-directed alkylating reagents, N-ethylmaleimide (NEM) and iodoacetamide (IAA), were used to show that at least one cysteinyl residue in the polyST was of critical importance for polysialylation, but of lesser importance for monosialylation, catalyzed by the alpha2,3-, alpha2,6-, and alpha2,8-monosialyltransferases (monoSTs).
TextSentencer_T12 1723-1778 Sentence denotes A sulfhydryl residue is implicated in chain initiation.
TextSentencer_T13 1779-1881 Sentence denotes Two important structural differences between the mono- and polySTs were revealed by sequence analyses.
TextSentencer_T14 1882-1999 Sentence denotes First, the polySTs contain heparin-like, positively charged amino acid clusters upstream of both sialylmotif L and S.
TextSentencer_T15 2000-2079 Sentence denotes Second, the polySTs contain a uniquely extended basic amino acid region (pI 11.
TextSentencer_T16 2080-2141 Sentence denotes 6-12.0) of 31 residues immediately upstream of sialylmotif S.
TextSentencer_T17 2142-2410 Sentence denotes This extended, positively charged region may function in the processive mechanism of polymerization by allowing nascent polySia chains to remain bound to the polyST during the repetitive addition of each new Sia residue to the nonreducing termini of the growing chain.
TextSentencer_T18 2411-2522 Sentence denotes The importance of these studies is that they provide new information on the enzymatic basis of polysialylation.
TextSentencer_T19 2523-2689 Sentence denotes They also reveal that sulfhydryl residues and extended basic amino acid domains are two structural features unique to polysialylation, in contrast to monosialylation.
TextSentencer_T20 2690-2862 Sentence denotes Both may be important distinguishing features between the classes of distributive (monoSTs) and processive polysialyltransferases, which have not been previously described.
T1 0-118 Sentence denotes Developmental expression and characterization of the alpha2,8-polysialyltransferase activity in embryonic chick brain.
T2 119-293 Sentence denotes The alpha2,8-polysialyltransferases (polySTs) from embryonic chick brain catalyze the alpha2,8-specific polysialylation of endogenous neural cell adhesion molecules (N-CAMs).
T3 294-383 Sentence denotes This posttranslation glycosylation decreases N-CAM-dependent cell adhesion and migration.
T4 384-485 Sentence denotes The enzymatic properties of the membrane-bound form of the polyST activity was investigated in vitro.
T5 486-633 Sentence denotes Our results show that the polyST activity was developmentally expressed with maximum specific activity appearing about 12 days after fertilization.
T6 634-881 Sentence denotes This time shortly precedes maximal expression of the cognate polysialylated N-CAMs. Kinetic studies showed the KMand Vmaxfor CMP-Neu5Ac were 133 microM and 0.13 microM/h, respectively, at pH 6.1, 33 degrees C. CMP-Neu5Gc was not a donor substrate.
T7 882-1017 Sentence denotes PolyST activity was increased 5- to 6-fold in the presence of 10 mM MnCl2,the preferred divalent cation, and 1 mM dithiothreitol (DTT).
T8 1018-1105 Sentence denotes Heparin (3 kDa) was a noncompetitive inhibitor of polysialylation with a Kiof 9 microM.
T9 1106-1342 Sentence denotes Based on the affinity of the enzyme for heparin, the polyST activity was partially purified ( approximately 30-fold) by heparin-Sepharose affinity chromatography, after differential solubilization with the zwitterionic detergent, CHAPS.
T10 1343-1722 Sentence denotes DTT and chemical modification studies using the thiol-directed alkylating reagents, N-ethylmaleimide (NEM) and iodoacetamide (IAA), were used to show that at least one cysteinyl residue in the polyST was of critical importance for polysialylation, but of lesser importance for monosialylation, catalyzed by the alpha2,3-, alpha2,6-, and alpha2,8-monosialyltransferases (monoSTs).
T11 1723-1778 Sentence denotes A sulfhydryl residue is implicated in chain initiation.
T12 1779-1881 Sentence denotes Two important structural differences between the mono- and polySTs were revealed by sequence analyses.
T13 1882-2079 Sentence denotes First, the polySTs contain heparin-like, positively charged amino acid clusters upstream of both sialylmotif L and S. Second, the polySTs contain a uniquely extended basic amino acid region (pI 11.
T14 2080-2410 Sentence denotes 6-12.0) of 31 residues immediately upstream of sialylmotif S. This extended, positively charged region may function in the processive mechanism of polymerization by allowing nascent polySia chains to remain bound to the polyST during the repetitive addition of each new Sia residue to the nonreducing termini of the growing chain.
T15 2411-2522 Sentence denotes The importance of these studies is that they provide new information on the enzymatic basis of polysialylation.
T16 2523-2689 Sentence denotes They also reveal that sulfhydryl residues and extended basic amino acid domains are two structural features unique to polysialylation, in contrast to monosialylation.
T17 2690-2862 Sentence denotes Both may be important distinguishing features between the classes of distributive (monoSTs) and processive polysialyltransferases, which have not been previously described.
T1 0-118 Sentence denotes Developmental expression and characterization of the alpha2,8-polysialyltransferase activity in embryonic chick brain.
T2 119-293 Sentence denotes The alpha2,8-polysialyltransferases (polySTs) from embryonic chick brain catalyze the alpha2,8-specific polysialylation of endogenous neural cell adhesion molecules (N-CAMs).
T3 294-383 Sentence denotes This posttranslation glycosylation decreases N-CAM-dependent cell adhesion and migration.
T4 384-485 Sentence denotes The enzymatic properties of the membrane-bound form of the polyST activity was investigated in vitro.
T5 486-633 Sentence denotes Our results show that the polyST activity was developmentally expressed with maximum specific activity appearing about 12 days after fertilization.
T6 634-843 Sentence denotes This time shortly precedes maximal expression of the cognate polysialylated N-CAMs. Kinetic studies showed the KMand Vmaxfor CMP-Neu5Ac were 133 microM and 0.13 microM/h, respectively, at pH 6.1, 33 degrees C.
T7 844-881 Sentence denotes CMP-Neu5Gc was not a donor substrate.
T8 882-1017 Sentence denotes PolyST activity was increased 5- to 6-fold in the presence of 10 mM MnCl2,the preferred divalent cation, and 1 mM dithiothreitol (DTT).
T9 1018-1105 Sentence denotes Heparin (3 kDa) was a noncompetitive inhibitor of polysialylation with a Kiof 9 microM.
T10 1106-1342 Sentence denotes Based on the affinity of the enzyme for heparin, the polyST activity was partially purified ( approximately 30-fold) by heparin-Sepharose affinity chromatography, after differential solubilization with the zwitterionic detergent, CHAPS.
T11 1343-1722 Sentence denotes DTT and chemical modification studies using the thiol-directed alkylating reagents, N-ethylmaleimide (NEM) and iodoacetamide (IAA), were used to show that at least one cysteinyl residue in the polyST was of critical importance for polysialylation, but of lesser importance for monosialylation, catalyzed by the alpha2,3-, alpha2,6-, and alpha2,8-monosialyltransferases (monoSTs).
T12 1723-1778 Sentence denotes A sulfhydryl residue is implicated in chain initiation.
T13 1779-1881 Sentence denotes Two important structural differences between the mono- and polySTs were revealed by sequence analyses.
T14 1882-1999 Sentence denotes First, the polySTs contain heparin-like, positively charged amino acid clusters upstream of both sialylmotif L and S.
T15 2000-2079 Sentence denotes Second, the polySTs contain a uniquely extended basic amino acid region (pI 11.
T16 2080-2141 Sentence denotes 6-12.0) of 31 residues immediately upstream of sialylmotif S.
T17 2142-2410 Sentence denotes This extended, positively charged region may function in the processive mechanism of polymerization by allowing nascent polySia chains to remain bound to the polyST during the repetitive addition of each new Sia residue to the nonreducing termini of the growing chain.
T18 2411-2522 Sentence denotes The importance of these studies is that they provide new information on the enzymatic basis of polysialylation.
T19 2523-2689 Sentence denotes They also reveal that sulfhydryl residues and extended basic amino acid domains are two structural features unique to polysialylation, in contrast to monosialylation.
T20 2690-2862 Sentence denotes Both may be important distinguishing features between the classes of distributive (monoSTs) and processive polysialyltransferases, which have not been previously described.

GlyCosmos15-Sentences

Id Subject Object Predicate Lexical cue
T1 0-118 Sentence denotes Developmental expression and characterization of the alpha2,8-polysialyltransferase activity in embryonic chick brain.
T2 119-293 Sentence denotes The alpha2,8-polysialyltransferases (polySTs) from embryonic chick brain catalyze the alpha2,8-specific polysialylation of endogenous neural cell adhesion molecules (N-CAMs).
T3 294-383 Sentence denotes This posttranslation glycosylation decreases N-CAM-dependent cell adhesion and migration.
T4 384-485 Sentence denotes The enzymatic properties of the membrane-bound form of the polyST activity was investigated in vitro.
T5 486-633 Sentence denotes Our results show that the polyST activity was developmentally expressed with maximum specific activity appearing about 12 days after fertilization.
T6 634-881 Sentence denotes This time shortly precedes maximal expression of the cognate polysialylated N-CAMs. Kinetic studies showed the KMand Vmaxfor CMP-Neu5Ac were 133 microM and 0.13 microM/h, respectively, at pH 6.1, 33 degrees C. CMP-Neu5Gc was not a donor substrate.
T7 882-1017 Sentence denotes PolyST activity was increased 5- to 6-fold in the presence of 10 mM MnCl2,the preferred divalent cation, and 1 mM dithiothreitol (DTT).
T8 1018-1105 Sentence denotes Heparin (3 kDa) was a noncompetitive inhibitor of polysialylation with a Kiof 9 microM.
T9 1106-1342 Sentence denotes Based on the affinity of the enzyme for heparin, the polyST activity was partially purified ( approximately 30-fold) by heparin-Sepharose affinity chromatography, after differential solubilization with the zwitterionic detergent, CHAPS.
T10 1343-1722 Sentence denotes DTT and chemical modification studies using the thiol-directed alkylating reagents, N-ethylmaleimide (NEM) and iodoacetamide (IAA), were used to show that at least one cysteinyl residue in the polyST was of critical importance for polysialylation, but of lesser importance for monosialylation, catalyzed by the alpha2,3-, alpha2,6-, and alpha2,8-monosialyltransferases (monoSTs).
T11 1723-1778 Sentence denotes A sulfhydryl residue is implicated in chain initiation.
T12 1779-1881 Sentence denotes Two important structural differences between the mono- and polySTs were revealed by sequence analyses.
T13 1882-2079 Sentence denotes First, the polySTs contain heparin-like, positively charged amino acid clusters upstream of both sialylmotif L and S. Second, the polySTs contain a uniquely extended basic amino acid region (pI 11.
T14 2080-2410 Sentence denotes 6-12.0) of 31 residues immediately upstream of sialylmotif S. This extended, positively charged region may function in the processive mechanism of polymerization by allowing nascent polySia chains to remain bound to the polyST during the repetitive addition of each new Sia residue to the nonreducing termini of the growing chain.
T15 2411-2522 Sentence denotes The importance of these studies is that they provide new information on the enzymatic basis of polysialylation.
T16 2523-2689 Sentence denotes They also reveal that sulfhydryl residues and extended basic amino acid domains are two structural features unique to polysialylation, in contrast to monosialylation.
T17 2690-2862 Sentence denotes Both may be important distinguishing features between the classes of distributive (monoSTs) and processive polysialyltransferases, which have not been previously described.

GlyCosmos15-Glycan

Id Subject Object Predicate Lexical cue image
T1 763-769 Glycan denotes Neu5Ac https://api.glycosmos.org/wurcs2image/latest/png/binary/G76685HR

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 112-117 Body_part denotes brain http://purl.obolibrary.org/obo/UBERON_0000955|http://purl.obolibrary.org/obo/UBERON_6110636
T3 186-191 Body_part denotes brain http://purl.obolibrary.org/obo/UBERON_0000955|http://purl.obolibrary.org/obo/UBERON_6110636
T5 253-264 Body_part denotes neural cell http://purl.obolibrary.org/obo/CL_0002319
T6 416-424 Body_part denotes membrane http://purl.obolibrary.org/obo/GO_0016020|http://purl.obolibrary.org/obo/UBERON_0000094|http://purl.obolibrary.org/obo/UBERON_0000158

CL-cell

Id Subject Object Predicate Lexical cue cl_id
T1 253-264 Cell denotes neural cell http://purl.obolibrary.org/obo/CL:0002319