PubMed:9579471 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/9579471","sourcedb":"PubMed","sourceid":"9579471","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/9579471","text":"Expression of gelatinases A and B, and TIMPs 1 and 2 during corneal wound healing.\nPURPOSE: To determine the expression of gelatinases A and B and the localization of tissue inhibitors of metalloproteinase (TIMP)-1 and TIMP-2 during the early stages of corneal wound healing in rats. METHODS. One eye each of 30 adult Sprague-Dawley rats was treated with excimer laser keratectomy; the other eye was untreated. Frozen sections of corneas obtained 6, 18, and 24 hours and 3 and 7 days after wounding were used to perform immunoconfocal microscopy and in situ hybridization.\nRESULTS: Gelatinase B was immunolocalized to the basement membrane zone and superficial stroma, and its mRNA was exclusively localized to basal epithelial cells migrating across the wound at 18 and 24 hours and 3 days after wounding. Tissue inhibitor of metalloproteinase-1 was also immunolocalized to the basement membrane zone and superficial stroma at the same time points. Gelatinase A was immunolocalized to the epithelium and stroma of normal corneas and was predominant in the basal epithelium and superficial stroma at 3 and 7 days after wounding. In situ hybridization confirmed gelatinase A expression by the epithelial cells and the stromal keratocytes. Tissue inhibitor of metalloproteinase-2 was immunolocalized to the epithelium in normal and wounded corneas. Intense TIMP-2 labeling of the basement membrane zone was noted 3 days after wounding.\nCONCLUSIONS: Unlike gelatinase A, gelatinase B is expressed exclusively by migrating basal epithelial cells after wounding. The matrix metalloproteinase-tissue inhibitor of metalloproteinase (MMP)/TIMP systems may play an important role in the early stages of corneal wound healing after excimer laser keratectomy.","tracks":[{"project":"PubMed_Structured_Abstracts","denotations":[{"id":"T1","span":{"begin":92,"end":572},"obj":"OBJECTIVE"},{"id":"T2","span":{"begin":582,"end":1433},"obj":"RESULTS"},{"id":"T3","span":{"begin":1447,"end":1748},"obj":"CONCLUSIONS"}],"attributes":[{"subj":"T1","pred":"source","obj":"PubMed_Structured_Abstracts"},{"subj":"T2","pred":"source","obj":"PubMed_Structured_Abstracts"},{"subj":"T3","pred":"source","obj":"PubMed_Structured_Abstracts"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"PubMed_Structured_Abstracts","color":"#c5ec93","default":true}]}]}}